CN112980965A - Kit and method for detecting human curiosity intensity genotype - Google Patents
Kit and method for detecting human curiosity intensity genotype Download PDFInfo
- Publication number
- CN112980965A CN112980965A CN201911284466.8A CN201911284466A CN112980965A CN 112980965 A CN112980965 A CN 112980965A CN 201911284466 A CN201911284466 A CN 201911284466A CN 112980965 A CN112980965 A CN 112980965A
- Authority
- CN
- China
- Prior art keywords
- kit
- curiosity
- genotype
- intensity
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kit and a method for detecting human curiosity intensity genotype. The invention adopts a multiple PCR amplification and electrophoresis method to analyze and identify the allelic polymorphism (SNP) of 3 genes: NRXN1, PCDH18 and TCERG1L, comprising the steps of: a) collecting the cells dropped off from the oral cavity of a testee, storing the cells in a collection card or extracting DNA nucleic acid from a blood sample; b) adding a saliva acquisition card sample or an extracted DNA sample of a testee into a reaction system for PCR amplification; c) running an amplification program; d) and carrying out electrophoretic analysis on the amplification products, and judging according to a peak pattern. The invention can synchronously detect the SNP of a plurality of genes related to a testee, and realizes the simplicity, high efficiency and specificity of detection. The intensity of curiosity of the subject is referenced by this analysis.
Description
Technical Field
The invention relates to the field of gene detection, in particular to a kit and a method for detecting human curiosity intensity genotype.
Background
Curiosity refers to the psychological tendency of an individual to pay attention, operate and ask questions when the individual encounters a novelty or is in a new external condition, and mainly represents the possibility that the individual likes novelty information. Curiosity as one of the intrinsic motivations for an individual to learn is not only the motivation of the individual to seek knowledge, but also an important feature of creative talents.
In the modern society, people have fully recognized the importance of curiosity on creation, innovation, invention and the like. Almost all scholars who study around creativity (including creativity, creative thinking, creative skills, personality traits of creators, etc.) have curiosity as the basic motivation for creativity, and have curiosity (and features related thereto, such as liking of complications, confusion, etc.) as important personality traits of high creativity. The american scholars hick-sumahi, in talking about the importance of curiosity, a factor of creative talents, also explicitly proposed that "the first step to creativity is curiosity and interest cultivation".
According to the Talent 5 five-occupational character evaluation developed based on the quintessence theory, the character traits of a person can be divided into five dimensions: aggressiveness, extroversion, accountability, pleasure and emotionality. Generally, people with higher extroversion scores have a stronger curiosity. They are more sensitive to changes in things and are more enthusiastic to learn new ideas and things. In operation, it may be more desirable to start the project with the head, accepting the challenge. People with strong curiosity can always find various opportunities for themselves, and to some extent, your curiosity determines your future.
The world is the integration of individual differences, and each human body has about 40 to 60 trillion cells, and each cell nucleus has a complete genome. The double helix structure is related to the characterization of appearance, character, capability and the like, and even lives, ages and deaths are all controlled by the double helix structure. More than 80% of gene polymorphisms in the human genome are Single Nucleotide Polymorphisms (SNPs), which refer to DNA sequence polymorphisms at the genome level caused by variations of a Single Nucleotide, including base transitions, transversions, deletions, and insertions. The human gene detection can diagnose diseases, predict disease risks, guide healthy life style, reduce unnecessary harm to human bodies, help people deeply interpret own unique genes, understand own personality and capability deviation, explore more possibilities and actively control human life. As a scientific, rapid and effective mode, gene detection can provide a lot of information for helping people to grow rapidly, and gene detection products become effective tools for exploring self and assisting decision. According to the talent theory, everyone has distinctive talent advantages, some talents are from genes, some talents are from acquired habits, the largest growth space of everyone lies in the field of fully exerting the strongest advantages of the talent, and gene detection helps the people to find unknown self and know the undetected advantages of the self. The method can help people to know the curiosity degree of the people by detecting the SNP locus change of the gene related to the curiosity, and actively and effectively measures are taken to stimulate the creative thinking of the people, improve the creativity and help people to open the infinite possibility of life.
In 2012, researchers in the united states developed a 11000-person research project that explored the relationship between human curiosity and loci. After a project researcher analyzes more than 120 million gene loci, 3 gene loci capable of influencing curiosity, such as NRXN1, PCDH18 and TCERG1L, are finally discovered, and the researcher discovers that the locus rs6754640 in the gene NRXN1 has more curiosity than A by analyzing the relation between the SNP loci and the ability; the rs987360 polymorphic T allele in the locus of the gene PCDH18 has more strange heart than C; the rs11018023 polymorphic A allele at site in TCERG1L has more petite heart than G. Studies have shown that people with great curiosity can to a large extent be interested in fresh things or problems encountered, and can be continuously concerned and explored in behavior without being liable to regress, even if they encounter difficulties. The analysis method can provide reference for the curiosity intensity of the testee.
Disclosure of Invention
The invention aims to provide a kit and a method for detecting the human curiosity intensity genotype, wherein the kit can detect related genes of a testee and give an analysis.
Specifically, the invention discloses a kit and a method for detecting the human curiosity intensity genotype, which comprises the following steps:
a) collecting the cells dropped off from the oral cavity of a testee, storing the cells in a collection card or extracting DNA nucleic acid from a blood sample;
b) adding a saliva acquisition card sample or an extracted DNA sample of a testee into a reaction system for PCR amplification;
c) running an amplification program;
d) carrying out electrophoretic analysis on the amplification product, and judging according to a peak pattern;
the kit comprises the following components: primer Mix, PCR reaction solution and DNA/RNA-free water.
Wherein the Primer Mix comprises a Primer group for amplifying 3 gene SNP polymorphic sites and a Primer group for 3 human genome DNA internal references and reaction internal references pcDNA, which are detailed in Table 1.
The kit can carry out synchronous multiplex PCR amplification on a detected sample, and can accurately detect the genotypes of detected genes NRXN1, PCDH18 and TCERG1L through the design of SNP primers: heterozygotes or homozygotes; the pcDNA is used for detecting a reaction system, monitoring whether the reaction system is effective or not and whether amplification is normal or not; 3 human genome DNA is internally referred to for detecting human samples, so that the human samples are guaranteed to be effective.
Wherein the PCR reaction solution comprises the following components: 2 XPCR buffer, DNA polymerase, dNTPs, potassium chloride, magnesium chloride, etc.
The PCR amplification reaction conditions of the kit are shown in Table 2.
TABLE 2 PCR amplification reaction conditions of the present invention
The amplification product of the kit is analyzed by electrophoresis; the preferred electrophoresis is capillary electrophoresis.
The kit can simultaneously amplify a plurality of loci, realizes the simplicity, high efficiency and specificity of detection, provides reference for the curiosity intensity of a testee by analyzing an amplification map, and is shown in table 3.
TABLE 3 detection site-character reference information of the present invention
Drawings
FIG. 1 is a negative control DNA/RNA-free water amplification map of the kit. Only characteristic peaks of pcDNA appear.
FIG. 2 is an amplification profile of a sample of exfoliated cells from the oral cavity of subject 1. The TCERG1L genotype is GA, PCDH18 is CT, NRXN1 is G, and pcDNA peak and characteristic peaks of human genome DNA (huDNA) internal reference-1, human genome DNA (huDNA) internal reference-3 and human genome DNA (huDNA) internal-8 appear.
Fig. 3 is an amplification map of a sample of exfoliated cells from the oral cavity of subject 2. The TCERG1L has genotype G, PCDH1 as CT and NRXN1 as G; the pcDNA peak and characteristic peaks of human genome DNA (huDNA) internal reference-1, human genome DNA (huDNA) internal reference-3 and human genome DNA (huDNA) internal-8 appear.
FIG. 4 is an amplification map of a DNA sample extracted from blood of subject 2, as shown in FIG. 3.
The specific implementation method comprises the following steps:
for a better understanding of the present invention, reference is made to the following detailed description and accompanying drawings. It is to be understood that these examples are for further illustration of the invention and are not intended to limit the scope of the invention. Moreover, it should be understood that the invention is not limited to the above-described embodiments, but is capable of various modifications and changes within the scope of the invention.
Detect whether to have the detect reagent box of curiosity intensity with birth, including box body and the reagent of depositing in the box body, including the following step:
a) collecting the cells dropped off from the oral cavity of a testee, storing the cells in a collection card or extracting DNA nucleic acid from a blood sample;
b) adding a saliva acquisition card sample or an extracted DNA sample of a testee into a reaction system for PCR amplification;
c) running an amplification program;
d) carrying out electrophoretic analysis on the amplification product, and judging according to a peak pattern;
the kit comprises the following components: primer Mix including Primer set, PCR reaction solution and DNA/RNA-free water.
The PCR reaction system Primer Mix comprises a Primer group for amplifying 5 SNP polymorphic sites, 3 personal genome references and pcDNA reaction references, and the details are shown in Table 1.
Wherein the PCR reaction solution comprises the following components: 2 XPCR buffer, DNA polymerase, dNTPs, potassium chloride, magnesium chloride, etc.
In the embodiment, the samples are DNA/RNA-free water, an oral cavity wall cast-off cell collecting card of the testee 1, an oral cavity wall cast-off cell collecting card of the testee 2 and a blood DNA extracting sample of the testee 2.
In the embodiment, the electrophoresis is to carry out electrophoresis on the amplification product through capillary electrophoresis, and an amplification map is given and analysis is given by combining analysis software, so as to judge the curiosity intensity of the testee.
Example one
(1) The selected samples were: DNA/RNA-free water.
(2) Architecture configuration
According to the specification, preparing a reaction system by the Primer Mix and the PCR reaction solution on ice according to the proportion of the specification, carrying out vortex mixing, centrifuging by a centrifuge, mixing uniformly by a gun head, and subpackaging.
(3) Adding the sample
According to the instructions, using a pipette to take a corresponding volume of DNA/RNA-free water, and adding into the reaction system which is divided.
(4) Amplification procedure
The amplification procedure on the PCR instrument is as in table 2.
(5) Detection of amplification product on 3500DX genetic analyzer
A sample mixture consisting of deionized formamide and an internal molecular weight standard in the system (Size-500) { (1. mu.L Size-500+ 12. mu.L deionized formamide) × (number of samples) }. Mix 9. mu.L of the sample mixture with 1. mu.L of the amplification product to avoid the formation of bubbles and to perform electrophoresis as soon as possible. Detection and analysis are carried out by an ABI 3500 genetic analyzer (purchased from ABI company of America), and specific analysis parameters are sample injection voltage: 1.2kv, sample injection time: 15s, electrophoresis time 1210-. The results of the detection are shown in FIG. 1.
(6) Analyzing data
The experiment uses DNA/RNA-free water, and the electrophoresis pattern should not have target peaks of each gene locus, and only has characteristic peaks of the pcDNA gene, as follows: the peak appearance corresponding to the pcDNA site is: singlet, showing pcDNA in the map;
as shown in FIG. 1, the electrophoretically analyzed DNA/RNA-free water-amplified product showed only the peak of the internal reference pcDNA of the reaction.
The experiment can effectively verify the amplification effectiveness of the reaction reagent and has no external pollution source.
Example two
(1) The selected samples were: samples of cells exfoliated from the oral wall of subjects 1 and 2.
(2) Sample collection
The collection type is as follows: cells are shed from the oral wall.
The acquisition method comprises the following steps: the saliva collecting rod is adopted to wipe the inner wall of the oral cavity back and forth for 4 times, the reverse side of the saliva collecting rod is used to wipe the inner wall of the oral cavity back and forth for 4 times, the saliva collecting rod is taken out, the saliva collecting rod is repeatedly pressed on a saliva sample collecting card, cells on the inner wall of the saliva are transferred to the saliva sample collecting card, and the collected saliva sample collecting card is dried in a pollution-free area.
Valid samples: the area of the saliva sample acquisition card with the pink area changed into light pink or white is the effective saliva sample area.
The sample selecting method comprises the following steps: manual punch sampling was performed using a dabber plastic punch (1.0 mm).
(3) Architecture configuration
According to the specification, preparing a reaction system by the Primer Mix and the PCR reaction solution on ice according to the proportion of the specification, carrying out vortex mixing, centrifuging by a centrifuge, mixing uniformly by a gun head, and subpackaging.
(4) Adding a sample: and taking 1-2 effective samples in the saliva card by using a puncher, and adding the effective samples into the prepared reaction system.
(5) Amplification procedure
The amplification procedure on the PCR instrument is as in table 2.
(6) Detection of amplification product on 3500DX genetic analyzer
A sample mixture consisting of deionized formamide and an internal molecular weight standard in the system (Size-500) { (1. mu.L Size-500+ 12. mu.L deionized formamide) × (number of samples) }. Mix 9. mu.L of the sample mixture with 1. mu.L of the amplification product to avoid the formation of bubbles and to perform electrophoresis as soon as possible. Detection and analysis are carried out by an ABI 3500 genetic analyzer (purchased from ABI company of America), and specific analysis parameters are sample injection voltage: 1.2kv, sample injection time: 15s, electrophoresis time 1210-. The detection results are shown in fig. 2 and fig. 3, respectively.
(7) Analyzing data
The experiment uses a sample of a testee, and the genotype of the testee is determined according to the position of each target peak in the map, so that the curiosity intensity of the testee is analyzed.
Fig. 2 is an analysis pattern of a saliva sample of the subject 1, and fig. 3 is an analysis pattern of a saliva sample of the subject 2.
The analysis according to FIG. 2 is as follows:
the electrophoresis pattern of the amplification product of the testee 1 shows the following characteristic peaks: the TCERG1L genotype is GA, PCDH18 site is CT, NRXN1 site is G, pcDNA peak and characteristic peak of ginseng-1 of human genome DNA (huDNA), ginseng-3 of human genome DNA (huDNA) and ginseng-8 of human genome DNA (huDNA).
Wherein locus G of the TCERG1L gene is shown as TCERG1L G, and locus A is shown as TCERG1L A; the PCDH18 gene, site C, is shown as PCDH18C, and site T is shown as PCDH 18T; NRXN1 gene site G is shown as NRXN 1G; the pcDNA locus is unimodal; human genomic DNA (huDNA) reference-1 is a single peak, and B1 is shown in the map; human genomic DNA (huDNA) reference-3 is a single peak, and B3 is shown in the map; the human genome DNA (huDNA) has a single peak at the reference-8 locus, and B8 is shown in the map.
Referring to table 3, test subject No. 1 had a heterozygous at TCERG1L site, PCDH18 site, a homozygous at NRXN1 site, and a genotype GG possessing NRXN1 site representing curiosity strength, and thus may possess a stronger curiosity.
Fig. 3 is an analytical profile of a saliva sample from subject 2.
The electrophoresis pattern of the amplification product of the testee 2 shows the following characteristic peaks: the TCERG1L has genotype G, PCDH1 as CT and NRXN1 as G; pcDNA peak, human genome DNA (huDNA) reference-1, human genome DNA (huDNA) reference-3, and human genome DNA (huDNA) reference-8 characteristic peak.
Wherein locus G of the TCERG1L gene is shown as TCERG1L G; the PCDH18 gene, site C, is shown as PCDH18C, and site T is shown as PCDH 18T; NRXN1 gene site G is shown as NRXN 1G; the pcDNA locus is unimodal; human genomic DNA (huDNA) reference-1 is a single peak, and B1 is shown in the map; human genomic DNA (huDNA) reference-3 is a single peak, and B3 is shown in the map; the human genome DNA (huDNA) has a single peak at the reference-8 locus, and B8 is shown in the map.
Referring to table 3, subject 2 is homozygous at TCERG1L and NRXN1, heterozygous at PCDH18, and deficient at TCERG1L a, indicating that subject 2 may have less curiosity than subject 1, and thus subject 2 may be less curious than subject 1.
EXAMPLE III
(1) The selected samples were: blood sample of subject 2.
(2) Sample type: a blood sample.
(3) And (3) extracting DNA: and (3) carrying out DNA extraction on the blood sample by using a nucleic acid extractor.
(4) Architecture configuration
According to the specification, preparing a reaction system by the Primer Mix and the PCR reaction solution on ice according to the proportion of the specification, carrying out vortex mixing, centrifuging by a centrifuge, mixing uniformly by a gun head, and subpackaging.
(5) Adding a sample: according to the instruction, a certain amount of the extracted DNA sample is taken by a pipette and added to the reaction system.
(6) Amplification procedure
The amplification procedure on the PCR instrument is as in table 2.
(7) Detection of amplification product on 3500DX genetic analyzer
A sample mixture consisting of deionized formamide and an internal molecular weight standard in the system (Size-500) { (1. mu.L Size-500+ 12. mu.L deionized formamide) × (number of samples) }. Mix 9. mu.L of the sample mixture with 1. mu.L of the amplification product to avoid the formation of bubbles and to perform electrophoresis as soon as possible. Detection and analysis are carried out by an ABI 3500 genetic analyzer (purchased from ABI company of America), and specific analysis parameters are sample injection voltage: 1.2kv, sample injection time: 15s, electrophoresis time 1210-. The results of the measurements are shown in FIG. 4.
(8) Analyzing data
FIG. 4 is an analysis chart of a blood extracted DNA sample of a subject 2, which lacks a TCERG1L G peak, a COX 10T peak and a NRXN 1A peak, and the other peaks are all present, and is consistent with FIG. 3, which shows that the detection kit is also applicable to the blood extracted DNA sample.
Claims (7)
1. A kit and a method for detecting the strength genotype of curiosity of human beings comprise the following steps:
a) collecting the cells dropped off from the oral cavity of a testee, storing the cells in a collection card or extracting DNA nucleic acid from a blood sample;
b) adding a saliva acquisition card sample or an extracted DNA sample of a testee into a reaction system for PCR amplification;
c) running an amplification program;
d) and carrying out electrophoretic fragment analysis on the amplified product, and judging according to a peak pattern.
The PCR reaction system comprises primer groups for amplifying 3 gene SNP polymorphic sites, 3 human genome DNA internal references and one PCR reaction internal reference, and the primer groups are shown in table 1, and the following primer sequence directions are all from 5 'end to 3' end.
TABLE 1 SNP detection sites, primer sequences and PCR amplification fragment lengths of the kit
2. The kit and method for detecting the human curiosity intensity genotype of claim 1, wherein the oral exfoliated cells are exfoliated cells on the inner wall of the oral cavity, are preserved on a cell collection card and can be directly used for PCR amplification.
3. The kit and method for detecting the human curiosity intensity genotype of claim 1, wherein the blood sample is a blood sample of a subject or a blood sample collected on a blood storage card, and the extracted DNA can be directly used for PCR amplification.
4. The kit and method for detecting the human curiosity intensity genotype as claimed in claim 1, wherein the reaction system comprises Primer Mix including Primer set, PCR reaction solution and DNA/RNA-free water.
5. The kit and method for detecting the human curiosity intensity genotype as claimed in claim 1, wherein the Primer Mix in the reaction system comprises primers for detecting all genotypes of SNPs of NRXN1, PCDH18 and TCERG1L, and comprises pcDNA and 3 human genomic DNA internal reference primers.
6. The kit and method for detecting the human curiosity intensity genotype as claimed in claim 1, wherein the PCR reaction solution in the reaction system comprises 2 XPCR buffer solution, DNA polymerase, dNTPs, potassium chloride, magnesium chloride, etc.
7. The kit and method for detecting the human curiosity intensity genotype of claim 1, wherein the amplification products are analyzed by electrophoresis; preferably the electrophoresis is capillary electrophoresis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911284466.8A CN112980965A (en) | 2019-12-13 | 2019-12-13 | Kit and method for detecting human curiosity intensity genotype |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911284466.8A CN112980965A (en) | 2019-12-13 | 2019-12-13 | Kit and method for detecting human curiosity intensity genotype |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112980965A true CN112980965A (en) | 2021-06-18 |
Family
ID=76341854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911284466.8A Withdrawn CN112980965A (en) | 2019-12-13 | 2019-12-13 | Kit and method for detecting human curiosity intensity genotype |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112980965A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113774168A (en) * | 2021-09-18 | 2021-12-10 | 北京艾瑞克阳医疗科技有限公司 | 2019 novel coronavirus, Deltay and lambda variant strain typing nucleic acid detection kit and detection method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373266A (en) * | 2010-08-09 | 2012-03-14 | 上海奇芯基因科技发展有限公司 | Detection chip and detection method of children customized education genes |
| CN109207579A (en) * | 2018-09-06 | 2019-01-15 | 宁波海尔施基因科技有限公司 | A kind of Multiple detection kit and application thereof detecting malignant fever tumor susceptibility gene |
-
2019
- 2019-12-13 CN CN201911284466.8A patent/CN112980965A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373266A (en) * | 2010-08-09 | 2012-03-14 | 上海奇芯基因科技发展有限公司 | Detection chip and detection method of children customized education genes |
| CN109207579A (en) * | 2018-09-06 | 2019-01-15 | 宁波海尔施基因科技有限公司 | A kind of Multiple detection kit and application thereof detecting malignant fever tumor susceptibility gene |
Non-Patent Citations (3)
| Title |
|---|
| "rs1101802", 《UCSC》 * |
| "rs6754640", 《UCSC》 * |
| "rs987360", 《UCSC》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113774168A (en) * | 2021-09-18 | 2021-12-10 | 北京艾瑞克阳医疗科技有限公司 | 2019 novel coronavirus, Deltay and lambda variant strain typing nucleic acid detection kit and detection method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104250663B (en) | The high-flux sequence detection method on methylated CpG island | |
| CN110747278B (en) | Composition, kit and method for detecting human ethanol metabolism gene polymorphism | |
| CN111088329B (en) | Fluorescence composite amplification system, kit and application thereof | |
| CN106939350A (en) | Detection kit, detection method and its application of thalassemia associated gene mutation | |
| CN113265476B (en) | Gene chip, molecular probe combination, kit and application for analyzing milk production performance of sheep | |
| CN114686597A (en) | SNP molecular marker for sex identification of salangid and application thereof | |
| CN108060237B (en) | Forensic medicine composite detection kit based on 55Y chromosome SNP genetic markers | |
| TW202022123A (en) | Cell-free dna damage analysis and its clinical applications | |
| CN103773859A (en) | SNP marker of mitochondria DNA related to asthenospermia with unknown clinical causes and application thereof | |
| CN113265475B (en) | Gene chip for analyzing sheep fat tail, molecular probe combination, kit and application | |
| CN112980965A (en) | Kit and method for detecting human curiosity intensity genotype | |
| CN101955996A (en) | Method for detecting single base Indel mutation | |
| CN107988385B (en) | Method for detecting marker of PLAG1 gene Indel of beef cattle and special kit thereof | |
| CN112980931A (en) | Kit and method for detecting human music epigenotype | |
| CN104342489A (en) | Method for detecting genotype of chicken beard | |
| CN112921099A (en) | Kit and method for detecting human scientific gas potential genotype | |
| CN106755525A (en) | A kind of probe and its application and kit for detecting mthfr gene mutation | |
| CN112941192A (en) | Kit and method for detecting potential genotype of human best partner | |
| CN112941194A (en) | Kit and method for detecting genotype of human body-building potential | |
| CN112941195A (en) | Kit and method for detecting human strongest brain potential genotype | |
| CN106434642B (en) | Primer and molecular biological method for identifying coilia ectenes and coilia brachypomus | |
| CN109486957A (en) | Pig multiple groups integrate accurate breeding method | |
| NL2030094B1 (en) | Snp molecular marker applicable to gender identification of silver arowana and application thereof | |
| CN112980964A (en) | Multiple gene detection kit for detecting human suntan skin and use method thereof | |
| CN112921100A (en) | Kit and method for detecting human pressure sensitivity genotype |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210618 |
|
| WW01 | Invention patent application withdrawn after publication |