CN102373266A - Detection chip and detection method of children customized education genes - Google Patents
Detection chip and detection method of children customized education genes Download PDFInfo
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Abstract
The invention discloses a detection chip of children customized education genes. The detection chip comprises a solid phase carrier and a probe. The probe is hybridized with a nucleotide sequence of customized education related genes of a detected children and/or a complementary sequence thereof, wherein the customized education related genes of a detected children comprises CHRM2, COMT, TPH2, ABCG1, MAOA, GSTP, MYOC, BDNF, ACTN3, ALDH2, Leptin, EAAT2, apoE, HTR2A, SNAP-25, D4DR, ADRB2, SLC6A2,and mstn genes. The invention also discloses a method for detecting children customized education genes by using the chip. With the gene chip provided by the invention, children potential capacity problems can be thoroughly studied; possible problems during a children growing process can be predicted in advance; blind training upon children can be avoided; and life career planning can be carried out upon children with pertinence.
Description
Technical field
The present invention relates to a kind of gene chip, relate in particular to a kind of children personalized education gene detecting chip and detection method thereof.
Background technology
Show with the twin research of dizygotic twins through enzygotic twins relatively, human body grow and the development and perfection of brain each several part function receive inherited genetic factors and Effect of Environmental and restriction, wherein about 30-60% receives the influence of inherited genetic factors.
As the saying goes: it takes ten years to grow trees, it takes ten years to grow trees, but a hundred to rear people.The cultivation child of science how teaches students in accordance with their aptitude truly realizing, each head of a family's responsibility still is not the responsibility of entire society yet.Educating one's children is a secular process, and the best period of cultivating child is 0～18 years old, and is the period of supporting child's most critical 0～6 years old the time.So four key factors one that child of culture successful becomes a useful person are starting points, the 2nd, direction, the 3rd, environment, the 4th, hope.Each factor here, the head of a family and teacher want to have accomplished completely to hold, and all be unable to do without the measurement to the genetic dominance gene in fact, and Here it is to the understanding of children's talent genes (being children personalized education gene) detection realistic meaning.
Therefore; Need utilize a kind of gene chip that detects children personalized education gene of biochip technology exploitation; To understand children's potential ability problem in depth; Predict that ahead of time the problem that children possibly run into comprises psychological problem in growing up, avoid cultivating blindly child, targetedly child is carried out the life occupational planning.
Summary of the invention
The technical problem that the present invention will solve provides a kind of children personalized education gene detecting chip; Can understand children's potential ability problem in depth; Predict that ahead of time the problem that children possibly run into comprises psychological problem in growing up; Avoid cultivating blindly child, targetedly child is carried out the life occupational planning.For this reason, the present invention also will provide the method for using the children personalized education gene of this chip detection.
In order to solve the problems of the technologies described above, the present invention realizes through following technical scheme:
In one aspect of the invention, a kind of children personalized education gene detecting chip is provided, has comprised solid phase carrier and probe, nucleotide sequence and/or its complementary sequence of said probe and children personalized education genes involved to be measured are hybridized, and said children personalized education genes involved to be measured comprises CHRM2, COMT; TPH2, ABCG1, MAOA, GSTP, MYOC, BDNF; ACTN3, ALDH2, Leptin, EAAT2, apoE, HTR2A; SNAP-25, D4DR, ADRB2, SLC6A2, mstn gene.
Solid phase carrier described in the present invention can be selected the known carrier in field for use, as long as said carrier is compatible with said reactant, it is just passable can not influence detected result.Preferably, solid phase carrier selection according to the invention is a kind of in slide, silicon chip, nitrocellulose filter, nylon membrane and the macromolecular material or their arbitrary combination.
Said probe can be DNA, RNA, DNA-RNA mosaic, PNA or derivatives thereof.The length of said probe is restriction not, combines as long as can accomplish with purpose nucleotide sequence specificity.Because different probe length has different influences to hybridization efficiency, signal specificity; The length of said probe is 14 base pairs usually at least; The longlyest generally be no more than 30 base pairs, and purpose nucleotide sequence complementary length is between 15-25 the base pair being the best.Self complementary sequence of said probe is most preferably less than 6 base pairs, in order to avoid influence hybridization efficiency.
The probe of gene chip of the present invention is DNA, comprising:
(1) with sequence shown in (a) SEQ ID NO:1～SEQ ID NO:8 of CHRM2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ ID NO:1～SEQ ID NO:8 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:1～SEQ ID NO:8;
(2) with sequence shown in (a) SEQ ID NO:9～SEQ ID NO:10 of COMT gene recombination to be measured; (b) complementary strand of sequence shown in SEQ ID NO:9～SEQ ID NO:10 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:9～SEQ ID NO:10;
(3) with sequence shown in (a) SEQ ID NO:11～SEQ ID NO:14 of TPH2 gene recombination to be measured; (b) complementary strand of sequence shown in the sequence shown in SEQ IDNO:11～SEQ ID NO:14 (c) has the sequence of at least 70% homology with sequence shown in the sequence shown in SEQ ID NO:11～SEQ ID NO:14;
(4) with sequence shown in (a) SEQ ID NO:15～SEQ ID NO:18 of ABCG1 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:15～SEQ ID NO:18 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:15～SEQ ID NO:18;
(5) with sequence shown in (a) SEQ ID NO:19～SEQ ID NO:20 of MAOA gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:19～SEQ ID NO:20 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:19～SEQ ID NO:20;
(6) with sequence shown in (a) SEQ ID NO:21～SEQ ID NO:22 of GSTP gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:21～SEQ ID NO:22 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:21～SEQ ID NO:22;
(7) with sequence shown in (a) SEQ ID NO:23～SEQ ID NO:26 of MYOC gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:23～SEQ ID NO:26 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:23～SEQ ID NO:26;
(8) with sequence shown in (a) SEQ ID NO:27～SEQ ID NO:28 of BDNF gene recombination to be measured; (b) complementary strand of sequence 8 shown in SEQ IDNO:27～SEQ ID NO:28 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:27～SEQ ID NO:28;
(9) with sequence shown in (a) SEQ ID NO:29～SEQ ID NO:30 of ACTN3 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:29～SEQ ID NO:30 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:29～SEQ ID NO:30;
(10) with sequence shown in (a) SEQ ID NO:31～SEQ ID NO:32 of ALDH2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:31～SEQ ID NO:32 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:31～SEQ ID NO:32;
(11) with sequence shown in (a) SEQ ID NO:33～SEQ ID NO:34 of Leptin gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:33～SEQ ID NO:34 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:33～SEQ ID NO:34;
(12) with sequence shown in (a) SEQ ID NO:35～SEQ ID NO:36 of EAAT2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:35～SEQ ID NO:36 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:35～SEQ ID NO:36;
(13) with sequence shown in (a) SEQ ID NO:37～SEQ ID NO:38 of apoE gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:37～SEQ ID NO:38 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:37～SEQ ID NO:38;
(14) with sequence shown in (a) SEQ ID NO:39 of HTR2A gene recombination to be measured, (b) complementary strand of sequence shown in the SEQ ID NO:39 (c) has the sequence of at least 70% homology with sequence shown in the SEQ ID NO:39;
(15) with sequence shown in (a) SEQ ID NO:40～SEQ ID NO:41 of SNAP-25 gene recombination to be measured; (b) complementary strand of sequence shown in SEQID NO:40～SEQ ID NO:41 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:40～SEQ ID NO:41;
(16) with sequence shown in (a) SEQ ID NO:42～SEQ ID NO:43 of D4DR gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:42～SEQ ID NO:43 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:42～SEQ ID NO:43;
(17) with sequence shown in (a) SEQ ID NO:44～SEQ ID NO:45 of ADRB2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:44～SEQ ID NO:45 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:44～SEQ ID NO:45;
(18) with sequence shown in (a) SEQ ID NO:46～SEQ ID NO:47 of SLC6A2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:46～SEQ ID NO:47 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:46～SEQ ID NO:47;
(19) with sequence shown in (a) SEQ ID NO:48～SEQ ID NO:49 of mstn gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:48～SEQ ID NO:49 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:48～SEQ ID NO:49.
Preferably, the probe of gene chip of the present invention is selected from sequence shown in SEQ ID NO:1～SEQ ID NO:49.
Said probe can be fixed on the solid phase carrier through connecting arm.Connecting arm can provide one the space is sterically hindered to reduce freely for probe forms double-stranded part; Carrying out [the Afanassiev V that helps hybridization; HanemannV; Wolfl S.Preparation of DNA and protein micro arrays on glass slides coated with an agarose film.Nucleic Acids Res.2000,28:e66; USA Patent No.5556752].Connecting arm is long more, and hybridization efficiency is high more.Typical connecting arm comprises 15～30 functional group length.Connecting arm can be selected the functional group of appropriate form for use, like the mosaic of Poly T (A, C or G), C atom or polyethylene glycol and Poly T (A, C or G), gather ethanol, gather cruel, gather ammonia, gather cruel and its compsn of sulfuric acid.
Said probe or connecting arm are fixed on the solid phase carrier through link molecule.Probe stationary can realize that for example, voltalef is surperficial through the C-C key to carrier; Or better use siloxane bond (glass or silicon-dioxide use when making upholder).The siloxane bond bonding can be through upholder and link molecule radical reaction completion such as Trichloromonosilane base or trialkoxysilyl.Aminoalkyl group silane, light basic alkyl silane, 2 one light ethyl one aminopropyl triethoxysilanes, light ethyl one aminopropyl triethoxysilane or light propyl-triethoxysilicane all are surface adsorption groups of great use.
Said probe can be modified, and modifying method can be 5 '-NH
2Modification, 5 '-SH modify, 5 '-Poly T (A, C or G) modifies, 5 '-biotin modification, 3 '-NH
2Modification, 3 '-SH modification, 3 '-Poly T (A, C or G) modification and 3 '-biotin modification etc.
Said probe can have one or several, even is all through mark, and that said mark comprises is fluorescein-labelled, biotin labeling, radioelement mark, enzyme labelling and FRET mark.
In another aspect of this invention, a kind of detection method of children personalized education gene detecting chip is provided, has comprised the steps:
1) extracts person under inspection's sample DNA;
2) design multiple PCR primer and SNP specific probe, the PCR primer has sequence shown in the SEQ ID NO:50-SEQ ID NO:99, and the SNP specific probe has sequence shown in the SEQ ID NO:1-SEQ ID NO:49;
3) sample DNA is carried out pcr amplification, purifying, the fragmentation and fluorescein-labelled of goal gene, said goal gene comprises CHRM2, COMT, TPH2, ABCG1; MAOA, GSTP, MYOC, BDNF, ACTN3; ALDH2, Leptin, EAAT2, apoE, HTR2A; SNAP-25, D4DR, ADRB2, SLC6A2, mstn gene; The used primer of this pcr amplification has nucleotide chain or its complementary strand of sequence shown in the SEQID NO:50-SEQ ID NO:99;
4) fluorescein-labeled PCR product carries out gene chip hybridization, the SNP site of testing goal gene.
The primer of the CHRM2 gene in the said step 3) is the primer of sequence shown in SEQ ID NO:50～57; The primer of COMT gene is the primer of sequence shown in SEQ ID NO:58～59; The primer of TPH2 gene is the primer of sequence shown in SEQ ID NO:60～63; The primer of ABCG1 gene is the primer of sequence shown in SEQ ID NO:64～67; The primer of MAOA gene is the primer of sequence shown in SEQ IDNO:68～69, and the primer of GSTP gene is the primer of sequence shown in SEQ ID NO:70～71, and the primer of MYOC gene is the primer of sequence shown in SEQ ID NO:72～75; The primer of BDNF gene is the primer of sequence shown in SEQ ID NO:76～77; The primer of ACTN3 gene is the primer of sequence shown in SEQ ID NO:78～79, and the primer of ALDH2 gene is the primer of sequence shown in SEQ ID NO:80～81, and the primer of Leptin gene is the primer of sequence shown in SEQ ID NO:82～83; The primer of EAAT2 gene is the primer of sequence shown in SEQ ID NO:84～85; The primer of apoE gene is the primer of sequence shown in SEQ ID NO:86～87, and the primer of HTR2A gene is the primer of sequence shown in SEQ ID NO:88～89, and the primer of SNAP-25 gene is the primer of sequence shown in SEQ ID NO:90～91; The primer of D4DR gene is the primer of sequence shown in SEQ ID NO:92～93; The primer of ADRB2 gene is the primer of sequence shown in SEQ ID NO:94～95, and the primer of SLC6A2 gene is the primer of sequence shown in SEQID NO:96～97, and the primer of mstn gene is the primer of sequence shown in SEQ ID NO:98～99.
Fragmentation in the said step 3) be with the PCR product of purifying after measuring concentration, carry out fragmentation with DNase I; Said fluorescein-labelled be to carry out fluorescein-labelled at 3 ' end fragmentation PCR product utilization deoxynucleotidyl transferase.
The preparation of said step 4) gene chip is to be downloaded to the probe that designs and synthesizes in advance on the solid phase carrier sheet base of slide or silicon chip material through contact point sample or ink jet type point of sample; Wherein, probe is the dna probe of sequence shown in SEQ ID NO:1～SEQ ID NO:49.
The SNP site of said step 3) goal gene comprises: the rs324650-T of CHRM2 gene, rs324650-A, rs2350780-G, rs2350780-A, rs2061174-G, rs2061174-A, rs8191992-T, rs8191992-A site, the rs4680-G of COMT gene, rs4680-A site, the rs4570625-G of TPH2 gene, rs4570625-T, rs4341581-G, rs4341581-T site; The rs225374-C of ABCG1 gene, rs225374-G, rs914189-C, rs914189-G site; The rs6323-G of MAOA gene, rs6323-T site, the rs1695-A of GSTP gene, rs1695-G site, the rs2421853-G of MYOC gene, rs2421853-A, rs235858-G, rs235858-A site; The rs6265-C of BDNF gene, rs6265-T site; The rs1815739-C of ACTN3 gene, rs1815739-T site, the rs671-G of ALDH2 gene, rs671-A site, the LeptinA1 of Leptin gene, LeptinG1 site; The EAAT2 gene-180A ,-the 180C site; The 112Cys of apoE gene, 112Arg site, the rs927544 site of HTR2A gene, the rs363039 site of SNAP-25 gene; The rs1800955 site of D4DR gene; The Arg16Gly site of ADRB2 gene, the rs2242446 site of SLC6A2 gene, the rs3791786 site of mstn gene.
The theoretical foundation in tumor susceptibility gene among the present invention and SNP site:
People's personality can be divided into hunting for novelty property (Novelty-Seeking; NS), hide nocuity (Harm-Avoidance; HA) and award dependency (Reward-Dependence; RD), relevant with Dopamine HCL (DA) neurotransmitter, serotonin (5-HT) neurotransmitter respectively with sympathin (NA) neurotransmitter.The main lust with brain of Dopamine HCL with feel relevant, transmit excited and happy information, also be addicted relevant.The brain DOPAMINE CONTENT IN RABBIT is many, and the people can become and be rich in curiosity, likes to take a risk, and is proactive.Love is exactly the result who produces a large amount of Dopamine HCL effects in the brain in fact.So smoking can increase the secretion of Dopamine HCL with taking drugs, habit-forming person is felt happy and excitement.If Dopamine HCL more after a little while, just can cause indecision and cold and detached individual character, lack tripping force and enthusiasm.Serotonin can directly influence people's mental functioning and physiological function, such as people's happiness, anger, grief and joy, sleep, appetite etc.The serotonin level is low in the blood, and the higher people's temper of catecholamine concentration is irritable easily, and personality is firmer, stiff than ordinary person, when anything crops up impulsion well.Otherwise serotonin concentration is higher in the blood, and people's disposition that catecholamine concentration is on the low side is gentleer than ordinary person, and is more calm when anything crops up, is difficult for giving way to impatience.Influencing individual personality in varying degrees with the heritable variation of these neurotransmitter genes involveds.Brain is the basic substance of all psychological activities, and brain structure is grown difference and not only influenced individual IQ, also influences individual personality simultaneously.Participate in growing with cerebral hippocampus like the BDNF gene, the low active individuality of BDNF is littler by 11% than the volume of normal people's cerebral hippocampus, thereby influences the part memory function, and relevant with anxiety, depression etc.
MYOC is a kind of cytoskeletal protein, and the form of participating in cilium appearance neuro epithelium (like sight sensor) takes place, and is distributed in part tissue of eye and the outer muscle tissue of eye.The MYOC transgenation is relevant with glaucoma myopia.
ALDH2 is the key enzyme of alcohol metabolism intermediate product acetaldehyde.ALDH2 function deficient patients because effective metabolism acetaldehyde causes acetaldehyde to be piled up in vivo, make individual the generation palpitate quickly, blush and malaise symptoms such as vomiting, and the normal person of ALDH2 function does not have this phenomenon, and relatively kind the drink.There is very big difference in human body to the metabolic detoxification ability of these toxin, causes some carcinogenss to reach tens of times at the activity difference of Different Individual, and the tumour that therefore carcinogens is caused exists genetic predisposition.The metabolic enzyme gene handicapped owing to do not separate toxenzyme in the body, can not detoxify to carcinogenic substance effectively, and is therefore responsive to the carcinogenic substance of cigarette, is the excessive risk crowd of smoking.
ACTN3 genes encoding αFu Jidongdanbai (ACTN3), ACTN3 show that body produces the ability of fast strength.In investigation, find the Australian Institute of Sport international sportsman; 18% normal people lacks ACTN3; The sportsmen of endurance eventses such as 24% long-distance running lacks ACTN3, and in the sportsmen of explosive power projects such as dash and weight lifting, has only 6% sportsmen to lack ACTN3; Particularly among the female athlete of explosive power project, all contain ACTN3.In the explosive power project, if the sportsmen lacks ACTN3, under the identical situation of the training quality of accepting, they can be near best sports achievement, but can not reach best all the time.Therefore,, formulate more rational training project, help improving sports achievement according to athletic inherited genetic factors.
D4DR gene (Dopamine Receptors D4, dopamine receptor D4) is positioned on the o.11 karyomit(e).The product of its manufacturing is a neurotransmitter important in the brain---the D4 subunit of the acceptor of Dopamine HCL.This subunit is the action target spot of some treatment sacred disease (like parkinsonism) medicines.Simultaneously, the length of one section Tumor-necrosis factor glycoproteins and people's behavior performance is closely bound up in this gene.The people who carries this gene length Tumor-necrosis factor glycoproteins is compared to the general population, and it is strong to show curiosity, overacfivity, and how moving, attention is concentrated or the like inadequately.So the explanation of DRD4 gene is people's characteristic of the curious degree of things to external world.
(monoamine oxidase A MAOA), is positioned on people's the sex chromosome X MAOA gene.The main effect of this gene is synthetic MAOA---the enzyme of a kind of neurotransmitter of degrading of coding.Such as its material such as Dopamine HCL, sympathin and serotonin in the brain of degrading.In case sudden change has taken place this gene, the ability of then making MAOA will descend, and some unnecessary neurotransmitters can not get degraded, can pile up gradually, has had influence on people's thinking, personality and even behavior performance.Therefore, MAOA is one of gene with the personality related intimate.
Thrombotonin is a kind of very important hormone and a neurotransmitter in the human body.And the albumen that this gene of TPH2 (TPH 2, tryptophan hydroxylase 2) coding is made one of the catalysis synthetic enzyme of thrombotonin just, and mainly in brain, express.This gene is positioned on No. 12 karyomit(e), and its sudden change can cause the defective of product TPH 2, and then causes the manufacturing capacity of thrombotonin to descend, and makes the people be partial to and shows moods such as pessimism, depression.
The vagusstoff of one of neurotransmitter (acetylcholine) affects the neurocyte in the cns.Neurocyte that can react to vagusstoff is called cholinergic nerve cell (cholinergic neuron), and this type cell all plays an important role aspect regulation and control brain physiological activity numerous.CHRM2 (muscarinic 2 for muscarinic cholinergic receptor 2, cholinergicreceptor) gene is positioned on No. 7 karyomit(e), expresses a kind of acetylcholine receptor, has participated in processes such as cynapse signal transduction, neuronal excitability.The activation of CHRM2 acceptor also can change slow β ripple and the δ ripple in the brain, and these brain waves are relevant with cognitive function (like decision-making and attention).The single base polymorphisms in some site has had influence on the higher cognitive process of brain in the CHRM2 gene.Therefore, this gene is one of gene with people's IQ related intimate.
The COMT gene is positioned on No. 22 karyomit(e), and the effect of coding synthetic enzyme is the degraded catecholamine.Catecholamine is a kind of neural type material that contains catechol and amido, comprises sympathin (NAd), suprarenin (Ad) and Dopamine HCL (DA).In people's neural system, they all are very important neurotransmitters, are the tie and the bridges of exchange message between the neurocyte.Certainly; Some catecholamines neurotransmitters have just lost effect after having accomplished the mission of oneself; This just needs the product of COMT gene---and catechol-O-methyltransferase is degraded, and avoids piling up in vivo forming a large amount of useless rubbish, keeps the metabolic balance of substance in vivo.Because the existence of transgenation makes that the activity of catechol-O-methyltransferase is different between the different people, the regulation and control that nerve signal is transduceed just exist difference, and then have had influence on the elaborative faculty of brain.So, must consider the factor of COMT gene when on molecular level, detecting people's IQ.
Beneficial effect of the present invention is:
1, solved child's genetics characteristic problem from the biology angle; Being to understand people's potential ability problem from the gene aspect, rather than resting on observation aspect or presentation aspect, is very forward-looking and guiding acting on; The utilization usual way can not be substituted at present in planning life process; This is a just instrument of a kind of rare amount for the teacher, for the head of a family, is the beacon light how a small cup cultivates the following passage to success of child.
2, make much of child's various actions and performance from the biology angle, the problem of especially worrying very much In the view of the adult possibly be a kind of necessary speciality that child will become a useful person future on the contrary.Such as: child has a wild nature, and is adventurous, often does the thing that a little common children are not bold enough to do.Do you how correctly find and cultivate such child? Only need carry out multianalysis to child's gene; Especially combinatory analysis; It is needed just can to find that this behavior of child belongs to following which kind of job characteristics; Just can educate towards this direction, be that the problem of shortcoming just can change into advantage once originally it seems.
3, can predict ahead of time that from the biology angle child plants the problem that the problem that possibly run into comprises in the heart of growing up; Some problem just can avoid so sorry or tragedy to take place even occur once just might causing child's lifelong regret or tragedy without redemption to take place on one's body your child through the gene test of children's talent as far as possible.Such as: child has calf love to add the susceptible depressed gene that add; The head of a family and teacher just need get ready 12 years old the time child soon; Take necessary mode to intervene; Comprise modes such as nutrition, medicine and education, the influence that just can avoid child to receive health sexual hormoue in the body development phase causes study, family and interpersonal relation and even social concern.
4, avoid cultivating blindly child from the biology angle, cultivate the very big pressure that child only can cause the body and mind aspect to child blindly.After detecting child's talent, the method for cultivating forest of just can sampling is pruned branch, and trying one's best alleviates child's various learning burdens and physical and psychological pressure.Because people's energy is limited, and school work is endless, people is as long as obtaining maximum performance aspect his advantage, and he just might have distinguished conduct.As: child finds to have legs to add skill genetic dominance gene; He just can select like gymnastics, long-distance running, sports events such as ball from screwing up discipline for a short time and often encouraging child's contest that takes exercises, and the unnecessary child of forcing participates in other various training seminares etc.Only in this way, child is bound to aspect sports, move ahead.
5, from the biology angle child is carried out the life occupational planning, perform sufficient preparation, comprise the preparation of aspects such as knowledge, technical ability, thought, ability for child walks out the social choice occupation one day in future.Such as; If child has the biology advantage of going into politics; That must be to join the party, participate in civil servant examination, the people of aspects such as contact China's political ideology system and personnel system and the necessary preparation that thing is done many aspects in university just, lays the first stone for child has one day and goes into politics in advance.
Description of drawings
Fig. 1 is the figure as a result of the electrophoresis detection after the multiplex PCR amplification among the embodiment 5;
Fig. 2 is the figure as a result of the electrophoresis detection behind the PCR product fragmentation among the embodiment 5;
Fig. 3 is detection chip results of hybridization figure among the embodiment 5.
Embodiment
Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed explanation.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.The experimental technique of unreceipted actual conditions in the following example; Usually by normal condition, people such as Sambrook for example, molecular cloning: laboratory manual (New York:Cold Spring Harbor LaboratoryPress; 1989) condition described in, or the condition of advising by manufacturer.
The extraction of embodiment 1. sample DNAs:
(1) (19 goal gene are: CHRM2, COMT, TPH2, ABCG1, MAOA from person under inspection's sample (healthy children) anticoagulation cirumferential blood, to utilize Qiagen FlexiGene DNA Kit (genomic dna purification kit) (51206) (this test kit is available from Qiagen company) extracting genomic dna; GSTP, MYOC, BDNF, ACTN3, ALDH2; Leptin, EAAT2, apoE, HTR2A, SNAP-25; D4DR, ADRB2, SLC6A2, mstn gene).Concrete grammar is: in 300 μ l blood samples, add 750 μ l Buffer FG1, turning upside down makes its mixing 5 times.Follow 12 centrifugal 1min under the 000rpm rotating speed.Outwell supernatant liquid after centrifugal, add 150 μ l Buffer FG2 and 1.5 μ l protein enzyme solutions again, vibration immediately is until deposition dissolving fully.Next centrifugal 3～5s, 65 ℃ of water-bath 5min then.After solution becomes olive-green from redness, add 150 μ l, 100% Virahol, fully put upside down centrifuge tube up and down, make its mixing, separate out until DNA, be macroscopic wire or bulk.Then 12, centrifugal 3min under the 000rpm rotating speed.Outwell supernatant liquid after centrifugal, add 150 μ l, 70% ethanol again, and vibration 5s.Then again 12, centrifugal 3min under the 000rpm rotating speed outwells supernatant liquid after centrifugal, and the natural air drying deposition all evaporates until all liquid.In centrifuge tube, add 200 μ l Buffer FG3 at last, vibration 5s, 65 ℃ of water-bath 10min make the DNA dissolving then.
(2) use ND-1000 nucleic acid concentration analyser quantitative to the extractive DNA of institute.DNA working fluid concentration correction places-20 ℃ of refrigerators to preserve to 10ng/ μ l.
Embodiment 2. design PCR (polymerase chain reaction) the primer and probe sequences:
Utilize www.autoprimer.com design multiplex PCR (polymerase chain reaction) primer and SNP specific probe (seeing table 1) to the SNP site.
Table 1
Embodiment 3: chip hybridization detects the SNP site
1. the preparation of gene chip
(1) probe dissolving
Every probe of sequence probe shown in SEQ ID NO:1～SEQ ID NO:49 is used the TE solution dilution, and final concentration is 10mM.With concentration be the probe of 10mM and PBS solution that concentration is 200mM in the medium volume mixture of 384 orifice plates, seal 384 orifice plates with adhesive sheet, vibration is 2 minutes under the room temperature, and is centrifugal ,-20 ℃ of preservations are used in order to point sample.
(2) point sample
The probe that designs and synthesizes in advance is downloaded on the solid phase carrier sheet base of materials such as slide, silicon chip through contact point sample or ink jet type point of sample.The sheet base adopts Cell Associates CSS-100 aldehyde radical sheet base; The point sample instrument of Ominigrid 100 models of GeneMachine company, humidity: 65-75% (being as the criterion with FullMoon sheet base), temperature is a point sample under 25 ℃ the condition; After point sample finishes; After placing half a hour, chip is taken out, drying at room temperature is preserved.
2. the processing of testing sample and mark
(1) amplification of goal gene
The primer (SEQ ID NO:50-SEQ ID NO:99 see table 1) corresponding with 19 goal gene carries out pcr amplification to sample DNA.Pcr amplification carries out with 30 μ l reaction systems; Reaction system is concentration 0.16 μ M, the genomic dna 10ng of 0.3mM dNTP, 10mM Tris-HCl, 50mM KCl, 2mM MgCl2,20%Q solution (Qiagen), upstream and downstream primer, Taq enzyme 0.6U (Takara).Use Touch-down PCR response procedures: 94 ℃ of sex change 5min; 94 ℃ of sex change 40s, 64 ℃ of annealing 1min, each circulation reduces by 0.5 ℃, and 72 ℃ are extended 50s, totally 10 circulations; 94 ℃ of sex change 40s then, 59 ℃ of annealing 40s, 72 ℃ are extended 50s, totally 30 circulations; Last 72 ℃ are extended 5min.PCR finishes the back and detects amplification with 1.5% sepharose.
When carrying out multi-PRC reaction, the primer of sequence shown in SEQ ID NO:50～SEQ ID NO:99 to be put into a reaction system increase, system is 50 μ l.The reaction system of multiplex PCR is: every kind of dNTP 0.3 μ mol/L, Tricine-KOH (PH=8.7) 40mmol/L, KCl 16mmol/L, MgCl
23.5mmol/L, BSA 3.75 μ g/ml, every primer 2 μ mol/L, DNA 80ng and 2.2 * Titanium Taq archaeal dna polymerase (Clontech, USA).Multi-PRC reaction condition: 95 ℃ of sex change 3min; 95 ℃ of sex change 30s, 66 ℃ of annealing 2min, 68 ℃ are extended 4min, totally 40 circulations; Last 68 ℃ prolong 10min.Behind the pcr amplification, get 3 μ l PCR reaction product and do agarose gel electrophoresis, these PCR products can be used for following hybridization step after treatment.
(2) PCR product purification and fragmentation
All PCR products of each sample mix, with QIA quick PCR Purification Kit (Qiagen, Cat.No.28106) purifying.The PCR product of purifying carries out fragmentation with DNase I (deoxyribonuclease I) after measuring concentration.The reaction system of fragmentation comprises: 30 μ l purified pcr products (10 μ g), 10 * DNase I damping fluid of 4 μ l, the DNaseI of 0.12 μ l, the ddH of 5.88 μ l
2O.Reaction conditions is that 37 ℃ of temperature are bathed 5min, 95 ℃ of 15min then.Product behind the fragmentation runs 4% sepharose, guarantees that most fragments is in 30-200 base pair.
(3) fluorescein-labelled
Utilize deoxynucleotidyl transferase to carry out fluorescein-labelled at 3 ' end; 40 μ l reaction systems of mark comprise: 25 μ l fragmentation PCR products; 5 * deoxynucleotidyl transferase damping fluid of 8 μ l; The CY3-N6-ddCTP of 1 μ l (1mM), the deoxynucleotidyl transferase of 3 μ l (20U/ μ l), the ddH of 3 μ l
2O.Reaction conditions is that 37 ℃ of temperature are bathed 120min, then 95 ℃ of heating 15min.
3. hybridization, washing and result detect
95 ℃ of sex change 10min of fluorescently-labeled PCR product place on ice immediately, are used for hybridization; Hybridization 20 μ l systems comprise: fluorescein-labeled PCR product 15 μ l, 20 * SSPE, 1.2 μ l, 1%Triton 0.2 μ l; 10 * Denhandts, 0.9 μ l, methane amide 0.5 μ l, ddH
2O 2.2 μ l.Reaction conditions is that 48 ℃ of temperature are bathed 120min, use in succession then 1 * lavation buffer solution I (5 * SSC, 0.1%SDS), (2 * SSC, 0.1%SDS) (1 * SSC) respectively washs 10min at 42 ℃ to 1 * lavation buffer solution II, uses ddH at last with 1 * lavation buffer solution III
2O washs 0.5min.
Chip after the washing after drying, scans (laser scanner that also can use other) with GenePix 4000B confocal laser scanner.Chip after the scanning hybridization obtains results of hybridization, handles image with GenePix Pro again and obtains data file, the data file is just analyzed can be obtained detected result then.
Embodiment 4: children's talent potential is estimated
According to embodiment 3 methods, 1000 routine normal children are analyzed.Through interactions such as a plurality of sites and environmental factorss, analyze children's talent potential, statistics shows that different children carry tumor susceptibility gene quantity and have significant statistical significance.
In the present embodiment, tumor susceptibility gene is meant that the mrna fraction that increases children's talent potential is 1 minute, and non-tumor susceptibility gene (being meant the gene that does not increase children's talent potential) is 0 minute.。
According to The above results, to formulate children's talent potential standard of index, and made corresponding children's talent potential assessment software (Shanghai Qixin Gene Technology Development Co., Ltd., Shanghai, China), standard is following: each tumor susceptibility gene is+1 minute; Non-tumor susceptibility gene is 0 minute.For a certain inborn potential, mark is 0, shows that this talent potential is common level, and mark is high more, shows that this talent potential is strong more.
Embodiment 5: person under inspection's inborn potential assessment
Sample a: person under inspection (male sex, 4 years old)
Detecting operation flow process according to embodiment 1-3 detects, and wherein, the electrophoresis detection result after this person under inspection's goal gene multiplex PCR amplification sees Fig. 1, and the electrophoresis detection result behind the PCR product fragmentation sees Fig. 2, and the detection chip results of hybridization is seen Fig. 3.The gained detected result is imported children's talent potential assessment software, and the children's talent potential index that obtains the person under inspection in the present embodiment is 2 minutes.
Sequence table
< 110>Shanghai Qixin Gene Technology Development Co., Ltd.
< 120>a kind of children personalized education gene detecting chip and detection method thereof
<130>NP-10-14376
<160>99
<170>PatentIn?version?3.3
<210>1
<211>18
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
< 223>probe
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atttccacaa?tattacac 18
<210>2
<211>18
<212>DNA
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<220>
<221>misc_feature
< 223>probe
<400>2
atttccacta?tattacac 18
<210>3
<211>17
<212>DNA
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<220>
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ctgctggacg?tacaaga 17
<210>4
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<212>DNA
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<400>4
ctgctggatg?tacaaga 17
<210>5
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<212>DNA
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ctgatgcacg?agaatag 17
<210>6
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<212>DNA
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ctgatgcatg?agaatag 17
<210>7
<211>17
<212>DNA
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attgtagaat?cagaagc 17
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<212>DNA
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attgtagatt?cagaagc 17
<210>9
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<212>DNA
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gtccttcacg?ccagcga 17
<210>10
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gtccttcatg?ccagcga 17
<210>11
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gacatattct?aattttg 17
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gacatattat?aattttg 17
<210>13
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aacgcctgca?atatcat 17
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<212>DNA
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aacgcctgaa?atatcat 17
<210>15
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gtcctgcccg?ctcatcg 17
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gtcctgcccc?ctcatcg 17
<210>17
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ctgagtctgc?ctcttct 17
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<211>17
<212>DNA
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ctgagtctcc?ctcttct 17
<210>19
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cattggaagc?cgctgaa 17
<210>20
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cattggaaga?cgctgaa 17
<210>21
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gagggagatg?tatttgc 17
<210>22
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gagggagacg?tatttgc 17
<210>23
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ccccagggct?cagttta 17
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ccccagggtt?cagttta 17
<210>25
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<212>DNA
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gcagcagacg?aaataaag 18
<210>26
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<212>DNA
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gcagcagatg?aaataaag 18
<210>27
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tcgaacacgt?gatagaag 18
<210>28
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tcgaacacat?gatagaag 18
<210>29
<211>17
<212>DNA
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tcgctctcgg?tcagcct 17
<210>30
<211>17
<212>DNA
< 213>artificial sequence
<220>
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tcgctctcag?tcagcct 17
<210>31
<211>18
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tttcacttca?gtgtatgc 18
<210>32
<211>18
<212>DNA
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<220>
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<400>32
tttcacttta?gtgtatgc 18
<210>33
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<220>
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<400>33
tggacttttt?ggatgggc 18
<210>34
<211>18
<212>DNA
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<400>34
tggactttct?ggatgggc 18
<210>35
<211>15
<212>DNA
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<220>
<221>misc_feature
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<400>35
gtgcgcgcct?gggga 15
<210>36
<211>15
<212>DNA
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<220>
<221>misc_feature
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<400>36
gtgcgcgccg?gggga 15
<210>37
<211>17
<212>DNA
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<220>
<221>misc_feature
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<400>37
gcggccgcac?acgtcct 17
<210>38
<211>17
<212>DNA
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<220>
<221>misc_feature
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<400>38
gcggccgcgc?acgtcct 17
<210>39
<211>20
<212>DNA
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<220>
<221>misc_feature
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<400>39
ctatgatacc?gtatcttctg 20
<210>40
<211>20
<212>DNA
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<220>
<221>misc_feature
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gcttctaccc?tgtcttgtcc 20
<210>41
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<212>DNA
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<400>41
gcttctactc?tgtcttgtcc 20
<210>42
<211>20
<212>DNA
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gtggagggcg?cgcacgaggt 20
<210>43
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<400>43
gtggagggtg?cgcacgaggt 20
<210>44
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<400>44
gccagcgaaa?gccccgagcc 20
<210>45
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gccagcgaag?gccccgagcc 20
<210>46
<211>21
<212>DNA
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gacgcgcgcc?cttttctggg?a 21
<210>47
<211>21
<212>DNA
< 213>artificial sequence
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<221>misc_feature
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<400>47
gacgcgcgct?cttttctggg?a 21
<210>48
<211>21
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>48
tgcaaaaatc?aaatatctta?a 21
<210>49
<211>21
<212>DNA
< 213>artificial sequence
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<221>misc_feature
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<400>49
gcaaaaatta?aatatcttaa?t 21
<210>50
<211>22
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>50
tacatgtctt?ctctgtcact?gg 22
<210>51
<211>26
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
< 223>primer
<400>51
ctcacaatag?ccctgaaaag?aatgac 26
<210>52
<211>23
<212>DNA
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<220>
<221>misc_feature
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tggagatgtc?atgaaagatt?tgc 23
<210>53
<211>25
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>53
aatgctaaag?tattgaggtg?gtatt 25
<210>54
<211>24
<212>DNA
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<220>
<221>misc_feature
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agcctaggtt?cttaagttct?gcat 24
<210>55
<211>26
<212>DNA
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<220>
<221>misc_feature
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<400>55
acccaaatag?acatcttgct?ctgagt 26
<210>56
<211>21
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>56
acaaaacgtg?caattcagga?g 21
<210>57
<211>21
<212>DNA
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<220>
<221>misc_feature
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cagagactga?taaaattgta?g 21
<210>58
<211>20
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>58
tcaccatcga?gatcaacccc 20
<210>59
<211>19
<212>DNA
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<220>
<221>misc_feature
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<400>59
acaacgggtc?aggcatgca 19
<210>60
<211>24
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>60
tccatataac?tctgcataga?ggca 24
<210>61
<211>20
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
< 223>primer
<400>61
gatatccatt?gcctcaagca 20
<210>62
<211>21
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>62
aggattcaac?gaggctaaga?g 21
<210>63
<211>20
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
< 223>primer
<400>63
gtgaagttgc?cgtgtcactc 20
<210>64
<211>24
<212>DNA
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<220>
<221>misc_feature
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<400>64
atgcttcaca?gtcctgatct?catt 24
<210>65
<211>21
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>65
cttccagaca?cgtttcccat?t 21
<210>66
<211>25
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
< 223>primer
<400>66
agccccccaa?ctctactact?taagc 25
<210>67
<211>24
<212>DNA
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<220>
<221>misc_feature
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<400>67
ttttgctagc?ctgaaaaacg?ccac 24
<210>68
<211>24
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>68
tttttcacgc?ctgccacaaa?gact 24
<210>69
<211>25
<212>DNA
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<220>
<221>misc_feature
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<400>69
acatgaataa?tagcagccta?ccctt 25
<210>70
<211>24
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>70
agtctctcat?ccttccacgc?acat 24
<210>71
<211>23
<212>DNA
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<220>
<221>misc_feature
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<400>71
catgacccgt?tacttggctg?gtt 23
<210>72
<211>24
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>72
acctgctagt?ttgacatgcc?taat 24
<210>73
<211>24
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<220>
<221>misc_feature
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<400>73
cagtttcaac?cgattctcct?gcct 24
<210>74
<211>25
<212>DNA
< 213>artificial sequence
<220>
<221>misc_feature
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<400>74
agtgtagagt?ggttctgttg?tggag 25
<210>75
<211>24
<212>DNA
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<220>
<221>misc_feature
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<400>75
aagctttgac?tcctttctca?tagg 24
<210>76
<211>20
<212>DNA
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<220>
<221>misc_feature
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<400>76
gaggcttgac?atcattggct 20
<210>77
<211>20
<212>DNA
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<220>
<221>misc_feature
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<400>77
cgtgtacaag?tctgcgtcct 20
<210>78
<211>21
<212>DNA
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<220>
<221>misc_feature
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<400>78
ctgttgcctg?tggtaagtgg?g 21
<210>79
<211>21
<212>DNA
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<220>
<221>misc_feature
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<400>79
tggtcacagt?atgcaggagg?g 21
<210>80
<211>23
<212>DNA
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<220>
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<400>80
gtcaactgct?atgatgtgtt?tgg 23
<210>81
<211>19
<212>DNA
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<220>
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<400>81
ccaccagcag?accctcaag 19
<210>82
<211>30
<212>DNA
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<400>82
tcttttctat?gtccaagctg?tgcccctgca 30
<210>83
<211>30
<212>DNA
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<220>
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<400>83
ttctactttg?tccccccata?ctctccttac 30
<210>84
<211>20
<212>DNA
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<220>
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gagcggcggg?gcctcttttc 20
<210>85
<211>20
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<400>85
tgcagccgct?gccacctgtg 20
<210>86
<211>24
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<400>86
agaccatgaa?ggagttgaag?gcct 24
<210>87
<211>20
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<400>87
cacctgctcc?ttcacctcgt 20
<210>88
<211>18
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<400>88
agcatgctca?tttaggtc 18
<210>89
<211>21
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gtaaaggctg?acagaacacc?a 21
<210>90
<211>21
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actgcgagat?aagaaatgtg?t 21
<210>91
<211>21
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<400>91
gttacaagga?atatgcccag?a 21
<210>92
<211>19
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<400>92
gcgggatgag?ctaggcgtc 19
<210>93
<211>21
<212>DNA
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<400>93
aaagacctga?gctcaggctc?t 21
<210>94
<211>19
<212>DNA
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<400>94
gagcccggta?acctgtcgt 19
<210>95
<211>20
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<400>95
acagcacgtc?cactgaggtc 20
<210>96
<211>21
<212>DNA
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<400>96
ctcagcctcg?gtgagttcaa?t 21
<210>97
<211>20
<212>DNA
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<400>97
ggcacgccga?ggctctgctt 20
<210>98
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Claims (9)
1. a children personalized education gene detecting chip comprises solid phase carrier and probe, it is characterized in that, nucleotide sequence and/or its complementary sequence of said probe and children personalized education genes involved to be measured are hybridized, and said children personalized education genes involved to be measured comprises CHRM2, COMT; TPH2, ABCG1, MAOA, GSTP, MYOC, BDNF; ACTN3, ALDH2, Leptin, EAAT2, apoE, HTR2A; SNAP-25, D4DR, ADRB2, SLC6A2, mstn gene.
2. detection chip as claimed in claim 1 is characterized in that, said probe is DNA, RNA, DNA-RNA mosaic, PNA or derivatives thereof.
3. detection chip as claimed in claim 2 is characterized in that, said probe is DNA, comprising:
(1) with sequence shown in (a) SEQ ID NO:1～SEQ ID NO:8 of CHRM2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ ID NO:1～SEQ ID NO:8 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:1～SEQ ID NO:8;
(2) with sequence shown in (a) SEQ ID NO:9～SEQ ID NO:10 of COMT gene recombination to be measured; (b) complementary strand of sequence shown in SEQ ID NO:9～SEQ ID NO:10 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:9～SEQ ID NO:10;
(3) with sequence shown in (a) SEQ ID NO:11～SEQ ID NO:14 of TPH2 gene recombination to be measured; (b) complementary strand of sequence shown in the sequence shown in SEQ IDNO:11～SEQ ID NO:14 (c) has the sequence of at least 70% homology with sequence shown in the sequence shown in SEQ ID NO:11～SEQ ID NO:14;
(4) with sequence shown in (a) SEQ ID NO:15～SEQ ID NO:18 of ABCG1 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:15～SEQ ID NO:18 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:15～SEQ ID NO:18;
(5) with sequence shown in (a) SEQ ID NO:19～SEQ ID NO:20 of MAOA gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:19～SEQ ID NO:20 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:19～SEQ ID NO:20;
(6) with sequence shown in (a) SEQ ID NO:21～SEQ ID NO:22 of GSTP gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:21～SEQ ID NO:22 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:21～SEQ ID NO:22;
(7) with sequence shown in (a) SEQ ID NO:23～SEQ ID NO:26 of MYOC gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:23～SEQ ID NO:26 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:23～SEQ ID NO:26;
(8) with sequence shown in (a) SEQ ID NO:27～SEQ ID NO:28 of BDNF gene recombination to be measured; (b) complementary strand of sequence 8 shown in SEQ IDNO:27～SEQ ID NO:28 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:27～SEQ ID NO:28;
(9) with sequence shown in (a) SEQ ID NO:29～SEQ ID NO:30 of ACTN3 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:29～SEQ ID NO:30 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:29～SEQ ID NO:30;
(10) with sequence shown in (a) SEQ ID NO:31～SEQ ID NO:32 of ALDH2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:31～SEQ ID NO:32 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:31～SEQ ID NO:32;
(11) with sequence shown in (a) SEQ ID NO:33～SEQ ID NO:34 of Leptin gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:33～SEQ ID NO:34 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:33～SEQ ID NO:34;
(12) with sequence shown in (a) SEQ ID NO:35～SEQ ID NO:36 of EAAT2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:35～SEQ ID NO:36 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:35～SEQ ID NO:36;
(13) with sequence shown in (a) SEQ ID NO:37～SEQ ID NO:38 of apoE gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:37～SEQ ID NO:38 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:37～SEQ ID NO:38;
(14) with sequence shown in (a) SEQ ID NO:39 of HTR2A gene recombination to be measured, (b) complementary strand of sequence shown in the SEQ ID NO:39 (c) has the sequence of at least 70% homology with sequence shown in the SEQ ID NO:39;
(15) with sequence shown in (a) SEQ ID NO:40～SEQ ID NO:41 of SNAP-25 gene recombination to be measured; (b) complementary strand of sequence shown in SEQID NO:40～SEQ ID NO:41 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:40～SEQ ID NO:41;
(16) with sequence shown in (a) SEQ ID NO:42～SEQ ID NO:43 of D4DR gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:42～SEQ ID NO:43 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:42～SEQ ID NO:43;
(17) with sequence shown in (a) SEQ ID NO:44～SEQ ID NO:45 of ADRB2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:44～SEQ ID NO:45 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:44～SEQ ID NO:45;
(18) with sequence shown in (a) SEQ ID NO:46～SEQ ID NO:47 of SLC6A2 gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:46～SEQ ID NO:47 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:46～SEQ ID NO:47;
(19) with sequence shown in (a) SEQ ID NO:48～SEQ ID NO:49 of mstn gene recombination to be measured; (b) complementary strand of sequence shown in SEQ IDNO:48～SEQ ID NO:49 (c) has the sequence of at least 70% homology with sequence shown in SEQ ID NO:48～SEQ ID NO:49.
4. detection chip as claimed in claim 3 is characterized in that, said probe is selected from: sequence shown in SEQ ID NO:1～SEQ ID NO:49.
5. the detection method like each described children personalized education gene detecting chip of claim 1-4 is characterized in that, comprises the steps:
1) extracts person under inspection's sample DNA;
2) design multiple PCR primer and SNP specific probe, the PCR primer has sequence shown in the SEQ ID NO:50-SEQ ID NO:99, and the SNP specific probe has sequence shown in the SEQ ID NO:1-SEQ ID NO:49;
3) sample DNA is carried out pcr amplification, purifying, the fragmentation and fluorescein-labelled of goal gene, said goal gene comprises CHRM2, COMT, TPH2, ABCG1; MAOA, GSTP, MYOC, BDNF, ACTN3; ALDH2, Leptin, EAAT2, apoE, HTR2A; SNAP-25, D4DR, ADRB2, SLC6A2, mstn gene; The used primer of this pcr amplification has nucleotide chain or its complementary strand of sequence shown in the SEQID NO:50-SEQ ID NO:99;
4) fluorescein-labeled PCR product carries out gene chip hybridization, the SNP site of testing goal gene.
6. the detection method of children personalized education gene detecting chip as claimed in claim 5; It is characterized in that; The primer of the CHRM2 gene in the said step 3) is the primer of sequence shown in SEQ ID NO:50～57; The primer of COMT gene is the primer of sequence shown in SEQID NO:58～59; The primer of TPH2 gene is the primer of sequence shown in SEQ ID NO:60～63, and the primer of ABCG1 gene is the primer of sequence shown in SEQ ID NO:64～67, and the primer of MAOA gene is the primer of sequence shown in SEQ ID NO:68～69; The primer of GSTP gene is the primer of sequence shown in SEQ ID NO:70～71; The primer of MYOC gene is the primer of sequence shown in SEQ ID NO:72～75, and the primer of BDNF gene is the primer of sequence shown in SEQ ID NO:76～77, and the primer of ACTN3 gene is the primer of sequence shown in SEQ ID NO:78～79; The primer of ALDH2 gene is the primer of sequence shown in SEQ IDNO:80～81; The primer of Leptin gene is the primer of sequence shown in SEQ ID NO:82～83, and the primer of EAAT2 gene is the primer of sequence shown in SEQ ID NO:84～85, and the primer of apoE gene is the primer of sequence shown in SEQ ID NO:86～87; The primer of HTR2A gene is the primer of sequence shown in SEQ ID NO:88～89; The primer of SNAP-25 gene is the primer of sequence shown in SEQ ID NO:90～91, and the primer of D4DR gene is the primer of sequence shown in SEQ ID NO:92～93, and the primer of ADRB2 gene is the primer of sequence shown in SEQ ID NO:94～95; The primer of SLC6A2 gene is the primer of sequence shown in SEQ IDNO:96～97, and the primer of mstn gene is the primer of sequence shown in SEQ ID NO:98～99.
7. the detection method of children personalized education gene detecting chip as claimed in claim 5 is characterized in that: the fragmentation in the said step 3) be with the PCR product of purifying after measuring concentration, carry out fragmentation with DNase I; Said fluorescein-labelled be to carry out fluorescein-labelled at 3 ' end fragmentation PCR product utilization deoxynucleotidyl transferase.
8. the detection method of children personalized education gene detecting chip as claimed in claim 5; It is characterized in that: the preparation of said step 4) gene chip is to be downloaded to the probe that designs and synthesizes in advance on the solid phase carrier sheet base of slide or silicon chip material through contact point sample or ink jet type point of sample; Wherein, probe is the dna probe of sequence shown in SEQ ID NO:1～SEQ ID NO:49.
9. the detection method of children personalized education gene detecting chip as claimed in claim 5; It is characterized in that: the SNP site of said step 3) goal gene comprises: the rs324650-T of CHRM2 gene, rs324650-A, rs2350780-G, rs2350780-A, rs2061174-G, rs2061174-A, rs8191992-T, rs8191992-A site, the rs4680-G of COMT gene, rs4680-A site, the rs4570625-G of TPH2 gene, rs4570625-T, rs4341581-G, rs4341581-T site; The rs225374-C of ABCG1 gene, rs225374-G, rs914189-C, rs914189-G site; The rs6323-G of MAOA gene, rs6323-T site, the rs1695-A of GSTP gene, rs1695-G site, the rs2421853-G of MYOC gene, rs2421853-A, rs235858-G, rs235858-A site; The rs6265-C of BDNF gene, rs6265-T site; The rs1815739-C of ACTN3 gene, rs1815739-T site, the rs671-G of ALDH2 gene, rs671-A site, the LeptinA1 of Leptin gene, LeptinG1 site; The EAAT2 gene-180A ,-the 180C site; The 112Cys of apoE gene, 112Arg site, the rs927544 site of HTR2A gene, the rs363039 site of SNAP-25 gene; The rs1800955 site of D4DR gene; The Arg16Gly site of ADRB2 gene, the rs2242446 site of SLC6A2 gene, the rs3791786 site of mstn gene.
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| CN2010102482123A CN102373266A (en) | 2010-08-09 | 2010-08-09 | Detection chip and detection method of children customized education genes |
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| CN104017878A (en) * | 2014-06-12 | 2014-09-03 | 山西大学 | Probe and method for detecting asthma susceptibility gene ADRB2 mutation sites |
| CN106086179A (en) * | 2016-06-16 | 2016-11-09 | 北京东方亚美基因科技研究院有限公司 | A kind of gene tester assessing child's natural endowment ability |
| CN109086563A (en) * | 2017-06-14 | 2018-12-25 | 达易特基因科技股份有限公司 | Using the adaptive physical education analysis method of genetic test |
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| CN112921100A (en) * | 2019-12-06 | 2021-06-08 | 宁波海尔施基因科技有限公司 | Kit and method for detecting human pressure sensitivity genotype |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104017878A (en) * | 2014-06-12 | 2014-09-03 | 山西大学 | Probe and method for detecting asthma susceptibility gene ADRB2 mutation sites |
| CN104017878B (en) * | 2014-06-12 | 2016-03-30 | 山西大学 | Detect probe and the method in asthma susceptibility gene ADRB2 mutational site |
| CN106086179A (en) * | 2016-06-16 | 2016-11-09 | 北京东方亚美基因科技研究院有限公司 | A kind of gene tester assessing child's natural endowment ability |
| CN109086563A (en) * | 2017-06-14 | 2018-12-25 | 达易特基因科技股份有限公司 | Using the adaptive physical education analysis method of genetic test |
| CN109251974A (en) * | 2018-02-14 | 2019-01-22 | 重庆京因生物科技有限责任公司 | APOE2 and APOE4 genotype quick detection kit based on POCT mode |
| CN112921100A (en) * | 2019-12-06 | 2021-06-08 | 宁波海尔施基因科技有限公司 | Kit and method for detecting human pressure sensitivity genotype |
| CN112980965A (en) * | 2019-12-13 | 2021-06-18 | 宁波海尔施基因科技有限公司 | Kit and method for detecting human curiosity intensity genotype |
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Application publication date: 20120314 |