CN112970441A - High-standard sterile virus-free control method for clonal propagation greenhouse - Google Patents
High-standard sterile virus-free control method for clonal propagation greenhouse Download PDFInfo
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- CN112970441A CN112970441A CN202110147704.1A CN202110147704A CN112970441A CN 112970441 A CN112970441 A CN 112970441A CN 202110147704 A CN202110147704 A CN 202110147704A CN 112970441 A CN112970441 A CN 112970441A
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- 238000000034 method Methods 0.000 title claims abstract description 43
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 54
- 230000001954 sterilising effect Effects 0.000 claims abstract description 27
- 241000196324 Embryophyta Species 0.000 claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000004140 cleaning Methods 0.000 claims abstract description 11
- 230000008569 process Effects 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 7
- 241000233866 Fungi Species 0.000 claims abstract description 7
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 7
- 241000700605 Viruses Species 0.000 claims abstract description 7
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000000638 solvent extraction Methods 0.000 claims abstract description 4
- 238000005507 spraying Methods 0.000 claims abstract description 4
- 239000000645 desinfectant Substances 0.000 claims description 23
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 15
- 239000003621 irrigation water Substances 0.000 claims description 15
- 229960002233 benzalkonium bromide Drugs 0.000 claims description 11
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 9
- 239000002316 fumigant Substances 0.000 claims description 9
- 230000000249 desinfective effect Effects 0.000 claims description 8
- QXJKBPAVAHBARF-BETUJISGSA-N procymidone Chemical compound O=C([C@]1(C)C[C@@]1(C1=O)C)N1C1=CC(Cl)=CC(Cl)=C1 QXJKBPAVAHBARF-BETUJISGSA-N 0.000 claims description 6
- 230000000391 smoking effect Effects 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 3
- 239000005747 Chlorothalonil Substances 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 241000723873 Tobacco mosaic virus Species 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 230000003139 buffering effect Effects 0.000 claims description 3
- 239000000460 chlorine Substances 0.000 claims description 3
- 229910052801 chlorine Inorganic materials 0.000 claims description 3
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 claims description 3
- CEJLBZWIKQJOAT-UHFFFAOYSA-N dichloroisocyanuric acid Chemical compound ClN1C(=O)NC(=O)N(Cl)C1=O CEJLBZWIKQJOAT-UHFFFAOYSA-N 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 238000003958 fumigation Methods 0.000 claims description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 3
- 238000003973 irrigation Methods 0.000 claims description 3
- 230000002262 irrigation Effects 0.000 claims description 3
- 239000011707 mineral Substances 0.000 claims description 3
- 239000002574 poison Substances 0.000 claims description 3
- 231100000614 poison Toxicity 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 235000020681 well water Nutrition 0.000 claims description 3
- 239000002349 well water Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 4
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 240000002234 Allium sativum Species 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 235000004611 garlic Nutrition 0.000 description 2
- 241001672694 Citrus reticulata Species 0.000 description 1
- 235000011511 Diospyros Nutrition 0.000 description 1
- 241000723267 Diospyros Species 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 235000021015 bananas Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
- C02F1/32—Treatment of water, waste water, or sewage by irradiation with ultraviolet light
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/76—Treatment of water, waste water, or sewage by oxidation with halogens or compounds of halogens
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Abstract
The invention discloses a clonal propagation greenhouse high-standard aseptic virus-free control method. In the invention, the greenhouse function partitioning is firstly carried out: the clonal production greenhouse functional subarea is provided with a necessary production area and a disinfection area; the disinfection area is divided into two buffer rooms by three doors; firstly, cleaning the greenhouse: removing weeds in the greenhouse and thoroughly cleaning the sanitation in the greenhouse, including a walkway, a passageway, a corridor, a buffer room and the like above and below the seedbed and between the seedbed and the passageway; s4, seedbed disinfection: spraying 0.7% sodium hypochlorite 12% -15% water, sterilizing all seedbeds in the greenhouse, and performing the next step 2-3 days after sterilization; by using a disinfection booth: weeds in the greenhouse are removed, the seedbed, the ground and the periphery of the greenhouse are disinfected and smoked in a matching manner, the infection sources of fungi, bacteria and viruses are controlled from various channels, the quality of the female parent is ensured, good conditions are created for the production of high-quality female parents, and therefore the survival rate in the propagation expanding culture process is improved.
Description
Technical Field
The invention belongs to the technical field of asexual culture, and particularly relates to a high-standard sterile virus-free control method for clone propagation in a greenhouse.
Background
Some fruit trees can not form seeds, such as bananas, seedless grapes, seedless persimmons, seedless mandarins and the like, the fruits are convenient to eat, the quality of the fruits is good, but because of the absence of the seeds, asexual propagation is required; some plants are difficult to seed, such as garlic, potato, white potato, etc., and must be grown under special conditions. For example, garlic must bloom and seed under mountain climates. After the white potato grows up, the plants are frozen in winter in the north. Therefore, it must be grown in a region where freezing damage does not occur at the later stage of growth to flower and set seeds, and this kind of plant also needs to be developed by asexual propagation.
However, in the common propagation process, the condition of infectious microbe infection can occur in the culture process, thereby affecting the survival rate in the culture process.
Disclosure of Invention
The invention aims to: in order to solve the problems, a high-standard sterile virus-free control method for a clonal propagation greenhouse is provided.
The technical scheme adopted by the invention is as follows: a clone propagation greenhouse high-standard aseptic virus-free control method comprises the following steps:
s1, first, partitioning greenhouse functions: the clonal production greenhouse functional subarea is provided with a necessary production area and a disinfection area; the disinfection area is divided into two buffer rooms by three doors;
s2, selecting and maintaining greenhouse materials, and then adopting a high-quality greenhouse film to ensure that each connection point is tight and seamless;
s3, before the greenhouse is used, the disinfection is carried out according to the following strict procedures and standards:
firstly, cleaning the greenhouse: removing weeds in the greenhouse and thoroughly cleaning the sanitation in the greenhouse, including a walkway, a passageway, a corridor, a buffer room and the like above and below the seedbed and between the seedbed and the passageway;
s4, seedbed disinfection: spraying 0.7% sodium hypochlorite 12% -15% water, sterilizing all seedbeds in the greenhouse, and performing the next step 2-3 days after sterilization;
s5, sterilizing the ground and the periphery of the greenhouse: 200 times of a happy nursery and 200 times of disinfection powder (active ingredient: sodium dichloroisocyanurate) are adopted to spray and disinfect the ground in a greenhouse, the upper and lower passageways of a seedbed and the walls around (dead angles such as a fan wet curtain);
s6, smoking: the mixed fumigant fumigation greenhouse of chlorothalonil and procymidone is characterized in that the dosage of the fumigant is 0.2-0.3g per cubic meter of space, (500 g of 30% procymidone fumigant 300-;
s7, pot: selecting a new unused flowerpot, using a sterile substrate, selecting well water which is rich in mineral substances and relatively clean for irrigation, and performing multiple filtration and resin softening on the irrigation water;
s8, disinfection: step one, filtering: filtering by a 3-micron filter; step two, ultraviolet sterilization: treating the filtered irrigation water by an ultraviolet lamp 420J/cm 2; step three, chlorine disinfection: adding sodium hypochlorite into the irrigation water sterilized by ultraviolet rays to enable the chloride ion concentration in the water to finally reach 0.1-0.2 ppm;
s9, selecting a breeding female parent;
s10, the operator requires: workers involved in clonal product production are strictly prohibited from having a history of smoking to avoid bringing tobacco mosaic virus into greenhouse shoe sole sterilization: when entering the buffering room, the shoe soles are disinfected and replaced: storing the carried articles or redundant clothes into a storage cabinet, and changing shoes or wearing shoe covers;
s12, washing hands: entering a second buffer room, and thoroughly cleaning hands by using a hand sanitizer; replacing the working clothes: the work clothes must be replaced every day;
s13, sole disinfection: sterilizing the sole again before entering the production area; sterilizing in operation;
s14, sterilizing the used tools: tools are fixedly used in each area, and appliances such as disinfectant, blades, scissors and the like fixed on the seedbed variety baffle can only be used in the corresponding area, and are placed back to the disinfection tank after being used, so that the appliances cannot be brought to other areas; disinfecting the blade or scissors.
In a preferred embodiment, in the step S1, a shoe sole sterilizing chamber, a shoe rack, a storage cabinet, etc. are provided in the first buffer room; the second buffer room is provided with a hand washing sink, a work clothes receiving table and an operation table (hand disinfection) area.
In a preferred embodiment, in step S2, the locations where ventilation is needed, such as the periphery of the greenhouse and the skylight, are tightly connected by using a high-density 80-mesh insect-proof net, and the greenhouse is periodically checked for the presence or absence of damage to the greenhouse film and the insect-proof net.
In a preferred embodiment, in step S2, the locations where ventilation is needed, such as the periphery of the greenhouse and the skylight, are tightly connected by using a high-density 80-mesh insect-proof net, and the greenhouse is periodically checked for the presence or absence of damage to the greenhouse film and the insect-proof net.
In a preferred embodiment, in step S8, the excessive amount of chloride ions may poison plants, so the concentration of chloride ions should be strictly controlled during the addition, and the content of chloride ions in the irrigation water should be constantly detected, and in order to ensure that the chloride ions in the irrigation water do not harm plants, the disinfected water is preferably left for 1 day before use.
In a preferred embodiment, in step S9, the female parent is the source of all high quality products, so it is important to select a female parent with high quality, and if a female parent with bacteria, fungi or even viruses is used, it will have a serious impact on the quality of the cutting, so extra care is needed in selecting the female parent to ensure that all the female parents are clean.
In a preferred embodiment, in step S9, the female parent is the source of all high quality products, so it is important to select a female parent with high quality, and if a female parent with bacteria, fungi or even viruses is used, it will have a serious impact on the quality of the cutting, so extra care is needed in selecting the female parent to ensure that all the female parents are clean.
In a preferred embodiment, in step S14, benzalkonium bromide (benzalkonium bromide) is diluted 15 times and used, the prepared disinfecting solution is poured into a disinfecting tank fixed in each area, and the scissors and the blades are soaked in the disinfecting solution (1 time per season, and the benzalkonium bromide is recommended to be used alternately with 70% alcohol).
In a preferred embodiment, in step S13, after touching the plants in the greenhouse, the hands are disinfected by the carried disinfectant, and after each operation of a fixed number of plants, the workers must disinfect the hands by the disinfectant to be able to proceed to the next operation.
In a preferred embodiment, in step S13, after touching the plants in the greenhouse, the hands are disinfected by the carried disinfectant, and after each operation of a fixed number of plants, the workers must disinfect the hands by the disinfectant to be able to proceed to the next operation.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. in the invention, by using the disinfection greenhouse: weeds in the greenhouse are removed, the seedbed, the ground and the periphery of the greenhouse are disinfected and smoked in a matching manner, the infection sources of fungi, bacteria and viruses are controlled from various channels, the quality of the female parent is ensured, good conditions are created for the production of high-quality female parents, and therefore the survival rate in the propagation expanding culture process is improved.
2. In the invention, the female parent is the source of all high-quality products, so that the high-quality female parent is selected, and all the selected female parents are ensured to be clean, thereby improving the sterile environment for culturing on the female parent and ensuring the sequential culturing.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to 7 embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A clone propagation greenhouse high-standard aseptic virus-free control method comprises the following steps:
s1, first, partitioning greenhouse functions: the clonal production greenhouse functional subarea is provided with a necessary production area and a disinfection area; the disinfection area is divided into two buffer rooms by three doors; in the step S1, a sole disinfection pool, a shoe rack, a storage cabinet and the like are arranged in the first buffer room; the second buffer room is provided with a hand washing sink, a work clothes receiving table, an operation table (hand disinfection) and other areas;
s2, selecting and maintaining greenhouse materials, and then adopting a high-quality greenhouse film to ensure that each connection point is tight and seamless; in the step S2, positions needing ventilation, such as the periphery of the greenhouse and a skylight, are tightly connected by adopting an 80-mesh high-density insect-proof net, and the closed condition of the greenhouse is periodically checked, so that the greenhouse film and the insect-proof net are damaged or not;
s3, before the greenhouse is used, the disinfection is carried out according to the following strict procedures and standards:
firstly, cleaning the greenhouse: removing weeds in the greenhouse and thoroughly cleaning the sanitation in the greenhouse, including a walkway, a passageway, a corridor, a buffer room and the like above and below the seedbed and between the seedbed and the passageway;
s4, seedbed disinfection: spraying 0.7% sodium hypochlorite 12% -15% water, sterilizing all seedbeds in the greenhouse, and performing the next step 2-3 days after sterilization; in the step S6, all materials entering the greenhouse can enter the greenhouse after being sprayed with disinfectant;
s5, sterilizing the ground and the periphery of the greenhouse: 200 times of a happy nursery and 200 times of disinfection powder (active ingredient: sodium dichloroisocyanurate) are adopted to spray and disinfect the ground in a greenhouse, the upper and lower passageways of a seedbed and the walls around (dead angles such as a fan wet curtain);
s6, smoking: the mixed fumigant fumigation greenhouse of chlorothalonil and procymidone is characterized in that the dosage of the fumigant is 0.2-0.3g per cubic meter of space, (500 g of 30% procymidone fumigant 300-;
s7, pot: selecting a new unused flowerpot, using a sterile substrate, selecting well water which is rich in mineral substances and relatively clean for irrigation, and performing multiple filtration and resin softening on the irrigation water;
s8, disinfection: step one, filtering: filtering by a 3-micron filter; step two, ultraviolet sterilization: treating the filtered irrigation water by an ultraviolet lamp 420J/cm 2; step three, chlorine disinfection: adding sodium hypochlorite into the irrigation water sterilized by ultraviolet rays to enable the chloride ion concentration in the water to finally reach 0.1-0.2 ppm; in the step S8, excessive chloride ions can poison plants, so the concentration of the chloride ions is strictly controlled during addition, the content of the chloride ions in the irrigation water is often detected, and the disinfected water is preferably placed for 1 day and then used in order to ensure that the chloride ions in the irrigation water can not damage the plants;
s9, selecting a breeding female parent; in the step S9, the female parent is the source of all high-quality products, so that the selection of the high-quality female parent is particularly important, and if the female parent with bacteria, fungi or even viruses is adopted, the quality of the cutting is seriously affected, so that extra caution is needed in the selection of the female parent to ensure that all the selected female parents are clean;
s10, the operator requires: workers involved in clonal product production are strictly prohibited from having a history of smoking to avoid bringing tobacco mosaic virus into greenhouse shoe sole sterilization: when entering the buffering room, the shoe soles are disinfected and replaced: storing the carried articles or redundant clothes into a storage cabinet, and changing shoes or wearing shoe covers; in the step S10, a reasonable supervision system is required for all the processes and links of the aseptic operation, so as to ensure the normal operation of the series of systems;
s12, washing hands: entering a second buffer room, and thoroughly cleaning hands by using a hand sanitizer; replacing the working clothes: the work clothes must be replaced every day; in step S12, the used work clothes are collectively cleaned by the laundry; hand disinfection: disinfecting hands by using a disinfectant, and carrying a bottle filled with the disinfectant with the hand: new benzalkonium bromide for hand disinfectant: water 1: 15 configuration;
s13, sole disinfection: sterilizing the sole again before entering the production area; sterilizing in operation; in the step S13, after touching the plants in the greenhouse, the hands are disinfected by the carried disinfectant, and after the worker finishes the operation of a fixed number of plants, the hands must be disinfected by the disinfectant to perform the next operation;
s14, sterilizing the used tools: tools are fixedly used in each area, and appliances such as disinfectant, blades, scissors and the like fixed on the seedbed variety baffle can only be used in the corresponding area, and are placed back to the disinfection tank after being used, so that the appliances cannot be brought to other areas; disinfecting blades or scissors; in the step S14, the benzalkonium bromide (benzalkonium bromide) is diluted 15 times and used, the prepared disinfectant is poured into a disinfection tank fixed in each area, and then the scissors and the blades are soaked in the disinfectant, and the disinfection is performed 1 time per quarter, and the benzalkonium bromide is recommended to be alternately used with alcohol with the concentration of 70%; by using a disinfection booth: weeds in the greenhouse are removed, the seedbed, the ground and the periphery of the greenhouse are disinfected and are smoked in a matched mode, the infection sources of fungi, bacteria and viruses are controlled from various channels, the quality of the female parent is guaranteed, good conditions are created for production of high-quality female parents, and therefore the survival rate in the propagation expanding culture process is improved; the female parent is the source of all high-quality products, so that the high-quality female parent is selected, and all the selected female parents are ensured to be clean, thereby improving the sterile environment for cultivation on the female parent and ensuring the orderly proceeding of subsequent cultivation.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or 014, and any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A clone propagation greenhouse high-standard sterile virus-free control method is characterized in that: the clone propagation greenhouse high-standard sterile virus-free control system comprises the following steps:
s1, first, partitioning greenhouse functions: the clonal production greenhouse functional subarea is provided with a necessary production area and a disinfection area; the disinfection area is divided into two buffer rooms by three doors;
s2, selecting and maintaining greenhouse materials, and then adopting a high-quality greenhouse film to ensure that each connection point is tight and seamless;
s3, before the greenhouse is used, the disinfection is carried out according to the following strict procedures and standards:
firstly, cleaning the greenhouse: removing weeds in the greenhouse and thoroughly cleaning the sanitation in the greenhouse, including a walkway, a passageway, a corridor, a buffer room and the like above and below the seedbed and between the seedbed and the passageway;
s4, seedbed disinfection: spraying 0.7% sodium hypochlorite 12% -15% water, sterilizing all seedbeds in the greenhouse, and performing the next step 2-3 days after sterilization;
s5, sterilizing the ground and the periphery of the greenhouse: 200 times of a happy nursery and 200 times of disinfection powder (active ingredient: sodium dichloroisocyanurate) are adopted to spray and disinfect the ground in a greenhouse, the upper and lower passageways of a seedbed and the walls around (dead angles such as a fan wet curtain);
s6, smoking: the mixed fumigant fumigation greenhouse of chlorothalonil and procymidone is characterized in that the dosage of the fumigant is 0.2-0.3g per cubic meter of space, (500 g of 30% procymidone fumigant 300-;
s7, pot: selecting a new unused flowerpot, using a sterile substrate, selecting well water which is rich in mineral substances and relatively clean for irrigation, and performing multiple filtration and resin softening on the irrigation water;
s8, disinfection: step one, filtering: filtering by a 3-micron filter; step two, ultraviolet sterilization: treating the filtered irrigation water by an ultraviolet lamp 420J/cm 2; step three, chlorine disinfection: adding sodium hypochlorite into the irrigation water sterilized by ultraviolet rays to enable the chloride ion concentration in the water to finally reach 0.1-0.2 ppm;
s9, selecting a breeding female parent;
s10, the operator requires: workers involved in clonal product production are strictly prohibited from having a history of smoking to avoid bringing tobacco mosaic virus into greenhouse shoe sole sterilization: when entering the buffering room, the shoe soles are disinfected and replaced: storing the carried articles or redundant clothes into a storage cabinet, and changing shoes or wearing shoe covers;
s12, washing hands: entering a second buffer room, and thoroughly cleaning hands by using a hand sanitizer; replacing the working clothes: the work clothes must be replaced every day;
s13, sole disinfection: sterilizing the sole again before entering the production area; sterilizing in operation;
s14, sterilizing the used tools: tools are fixedly used in each area, and appliances such as disinfectant, blades, scissors and the like fixed on the seedbed variety baffle can only be used in the corresponding area, and are placed back to the disinfection tank after being used, so that the appliances cannot be brought to other areas; disinfecting the blade or scissors.
2. The method of claim 1, wherein the method comprises the following steps: in the step S1, a sole disinfection pool, a shoe rack, a storage cabinet and the like are arranged in the first buffer room; the second buffer room is provided with a hand washing sink, a work clothes receiving table, an operation table (hand disinfection) and other areas.
3. The method of claim 1, wherein the method comprises the following steps: in the step S2, the positions to be ventilated, such as the periphery of the greenhouse and the skylight, are tightly connected by using a high-density insect-proof net with 80 meshes, and the sealing condition of the greenhouse is periodically checked to see whether the greenhouse film and the insect-proof net are damaged or not.
4. The method of claim 1, wherein the method comprises the following steps: in step S6, all the materials entering the greenhouse must be sprayed with the disinfectant solution before entering the greenhouse.
5. The method of claim 1, wherein the method comprises the following steps: in step S8, excessive chloride ions poison plants, so the concentration of chloride ions should be strictly controlled during addition, and the content of chloride ions in the irrigation water should be frequently detected, so that the disinfected water is preferably left for 1 day before use in order to ensure that the chloride ions in the irrigation water do not harm plants.
6. The method of claim 1, wherein the method comprises the following steps: in the step S9, the female parent is the source of all high quality products, so it is important to select a high quality female parent, and if a female parent with bacteria, fungi or even viruses is used, it will have a serious impact on the quality of the cutting, so extra caution is needed in selecting the female parent to ensure that all the selected female parents are clean.
7. The method of claim 1, wherein the method comprises the following steps: in step S10, a reasonable supervision system is required for all the aseptic operation processes and links, so as to ensure the normal operation of the series of systems.
8. The method of claim 1, wherein the method comprises the following steps: in step S14, benzalkonium bromide (benzalkonium bromide) is diluted 15 times and used, the prepared disinfectant is poured into a disinfection tank fixed in each area, and then the scissors and the blades are soaked in the disinfectant, and disinfection is performed 1 time per quarter, and the benzalkonium bromide is recommended to be used alternately with alcohol with a concentration of 70%.
9. The method of claim 1, wherein the method comprises the following steps: in step S13, the hands are disinfected by the carried disinfectant after touching the plants in the greenhouse, and after the worker finishes the operation of a fixed number of plants, the hands must be disinfected by the disinfectant to perform the next operation.
10. The method of claim 1, wherein the method comprises the following steps: in step S12, the used work clothes are collectively cleaned by the laundry; hand disinfection: disinfecting hands by using a disinfectant, and carrying a bottle filled with the disinfectant with the hand: new benzalkonium bromide for hand disinfectant: water 1: 15 configuration.
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| CN202110147704.1A CN112970441A (en) | 2021-02-03 | 2021-02-03 | High-standard sterile virus-free control method for clonal propagation greenhouse |
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| CN107970473A (en) * | 2017-11-21 | 2018-05-01 | 张掖市农业科学研究院 | Vegetable base sterilization method and production base |
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| CN107970473A (en) * | 2017-11-21 | 2018-05-01 | 张掖市农业科学研究院 | Vegetable base sterilization method and production base |
| CN208372175U (en) * | 2017-11-21 | 2019-01-15 | 张掖市农业科学研究院 | Disinfection room and base greenhouse |
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