CN112963984A - Multichannel refrigerating device for microtube electrophoresis separation system and working method thereof - Google Patents
Multichannel refrigerating device for microtube electrophoresis separation system and working method thereof Download PDFInfo
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- CN112963984A CN112963984A CN202110204040.8A CN202110204040A CN112963984A CN 112963984 A CN112963984 A CN 112963984A CN 202110204040 A CN202110204040 A CN 202110204040A CN 112963984 A CN112963984 A CN 112963984A
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
- F25B23/00—Machines, plants or systems, with a single mode of operation not covered by groups F25B1/00 - F25B21/00, e.g. using selective radiation effect
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Abstract
The invention relates to a multi-channel refrigerating device for a microtube electrophoresis separation system and a working method thereof, wherein the multi-channel refrigerating device comprises a separation channel arranged on a substrate and a component collecting channel vertically communicated with the separation channel; a first binding post and a second binding post are respectively arranged at two ends of the separation channel, and a capillary tube for sample introduction is communicated with the separation channel between the component collection channel and the second binding post; and a third binding post and a fourth binding post are respectively arranged at two ends of the component collecting channel. During the use, connect different high pressure and maintenance voltage through first terminal, second terminal, third terminal and fourth terminal, in time spill the joule heat that produces in the separation channel, reduce the temperature and the conductivity of operation buffer solution in the separation channel, and then reduce the change that joule heat brought voltage and electric current, realize the separation of sample component, the device has the advantage that improves separation efficiency, steady current voltage.
Description
Technical Field
The invention relates to the technical field of electrophoretic separation, in particular to a multichannel refrigerating device for a microtubule electrophoretic separation system and a working method thereof.
Background
The charged particles move under the action of an electric field towards an electrode of opposite polarity, known as electrophoresis. A technique of achieving separation by utilizing a difference in moving speed of charged particles in an electric field is called an electrophoresis technique.
Capillary Electrophoresis (CE), also known as high-efficiency capillary electrophoresis, is a new technology for performing efficient and rapid separation of ions or charged particles in a capillary according to different mobility or distribution coefficients by using a high-voltage direct-current electric field as a driving force.
The prior capillary electrophoresis separation technology has the following problems: (1) the capillary tube is easy to generate Joule heat in the electrophoresis process, a built-in cooling system is usually adopted in the prior art to take away the Joule heat generated in the electrophoresis process, the Joule heat in the capillary tube is easy to generate in the prior cooling system, and is difficult to dissipate in time, the Joule heat is accumulated in the capillary tube, so that the temperature of running buffer solution in two ends of the capillary tube is increased, the conductivity is increased, and the voltage and the current deviate from the linear relation; (2) due to the limitation of the diameter of the capillary tube, the sample carrying amount of the sample is small, the electrophoretic separation efficiency is low, and the high-efficiency separation cannot be realized.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a multichannel refrigeration device for improving the microtube electrophoretic separation efficiency and stabilizing the current and voltage and a working method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
a multi-channel refrigerating device for a microtube electrophoresis separation system comprises a separation channel arranged on a substrate and a component collection channel vertically communicated with the separation channel;
a first binding post and a second binding post are respectively arranged at two ends of the separation channel, and a capillary tube for sample introduction is communicated with the separation channel between the component collection channel and the second binding post;
a third binding post and a fourth binding post are respectively arranged at two ends of the component collecting channel;
the separation channel is a millimeter-sized micro-tube, and the tube diameter of the micro-tube is larger than that of the capillary tube.
Further, the matrix is polydimethylsiloxane.
A working method of a multi-channel refrigerating device for a microtube electrophoresis separation system specifically comprises the following steps;
step 1: firstly, injecting a sample to be separated into a separation channel through a capillary, then switching on a second binding post to a high-voltage power supply, grounding a first binding post, and connecting a third binding post and a fourth binding post to a maintaining voltage power supply for separating sample components;
step 2: after the separation is finished, the first binding post is connected with a voltage maintaining power supply, and the third binding post is grounded and used for transferring and collecting a group of sample components after the components are separated; and connecting the third terminal with a maintaining voltage, and connecting the fourth terminal with the ground for transferring and collecting another group of sample components after the separated components are separated.
Compared with the prior art, the invention has the beneficial effects that:
1) according to the invention, the first binding post, the second binding post, the third binding post and the fourth binding post are connected with different high-voltage power supplies and voltage-maintaining power supplies, so that Joule heat generated in the separation channel is dissipated in time, the temperature and the conductivity of running buffer solutions at two ends of the separation channel are reduced, further the change of voltage and current caused by Joule heat is reduced, and the separation of sample components with stable current and voltage is realized.
2) The invention adopts the multi-channel high-voltage power supply as the driving force of the whole microtube electrophoresis system, reasonably regulates and controls the buffer solution in the two ends of the separation channel, realizes the shunt collection of sample components through the component collection channel and improves the separation efficiency of the sample components.
3) The invention creatively and organically combines a large tube containing a millimeter-scale separation channel with a high-resolution capillary tube to replace the capillary tube technology, so that the sample carrying amount is increased by dozens of times, and the efficiency is greatly improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
in the figure: 1-substrate, 2-separation channel, 3-first terminal, 4-second terminal, 5-capillary, 6-component collection channel, 7-third terminal, 8-fourth terminal.
Detailed Description
The present invention will be described in further detail with reference to the following examples, which are not intended to limit the invention thereto.
As shown in figure 1, the invention discloses a multichannel refrigerating device for a microtubule electrophoresis separation system, which comprises a substrate 1 made of polydimethylsiloxane, wherein a millimeter-scale microtubule separation channel 2 is arranged on the substrate 1, and a first binding post 3 and a second binding post 4 are respectively arranged at two ends of the separation channel 2.
The substrate 1 is also provided with a component collecting channel 6 vertically communicated with the separation channel 2, and both ends of the component collecting channel 6 are respectively provided with a third binding post 7 and a fourth binding post 8.
A capillary 5 for sample introduction is communicated on the separation channel 2 between the component collection channel 6 and the second binding post 4, and the pipe diameter of the capillary 5 is smaller than that of the separation channel 2.
The working method comprises the following steps: the method specifically comprises the following steps; step 1: firstly, a sample to be separated is injected into the separation channel 2 through the capillary 5, then the second binding post 4 is connected with a high-voltage power supply, the first binding post 3 is grounded, and the third binding post 7 and the fourth binding post 8 are both connected with a maintaining voltage power supply for separating sample components.
Step 2: and after the separation is finished, the first binding post 3 is connected with a voltage maintaining power supply, and the third binding post 7 is grounded and used for transferring and collecting a group of sample components after the components are separated. Terminal three 7 was connected to a sustaining voltage and terminal four 8 was connected to ground for transfer of another set of sample fractions after collection of the separated fractions.
In conclusion, the device adopts a multi-channel high-voltage power supply as the driving force of the whole microtube electrophoresis separation system, reasonably regulates and controls the buffer solution in the two ends of the separation channel 2, realizes component shunt collection through the component collection channel 6 and improves the separation efficiency. Further connect different high voltage power supply and maintenance voltage power supply through first terminal 3, second terminal 4, third terminal 7 and fourth terminal 8, in time spill the joule heat that produces in separation channel 2, reduce the temperature and the conductivity of operation buffer solution in the separation channel 2 both ends, and then reduce joule heat and bring the change of voltage and electric current, realize the sample component separation of stable current voltage.
Claims (3)
1. A multichannel refrigerating plant for microtube electrophoresis separation system which characterized in that: comprises a separation channel (2) arranged on a substrate (1) and a component collecting channel (6) vertically communicated with the separation channel (2);
a first binding post (3) and a second binding post (4) are respectively arranged at two ends of the separation channel (2), and a capillary (5) for sample introduction is communicated on the separation channel (2) between the component collection channel (6) and the second binding post (4);
a third binding post (7) and a fourth binding post (8) are respectively arranged at two ends of the component collecting channel (6);
the separation channel (2) is a millimeter-sized micro-tube, and the tube diameter of the micro-tube is larger than that of the capillary (5).
2. The multi-channel refrigeration device for the microtube electrophoretic separation system as claimed in claim 1, wherein: the substrate (1) is polydimethylsiloxane.
3. A method for operating a multi-channel refrigeration device for microtube electrophoresis separation system according to claim 1 or 2, wherein: the method specifically comprises the following steps;
step 1: firstly, a sample to be separated is injected into a separation channel (2) through a capillary tube (5), then a second wiring terminal (4) is connected with a high-voltage power supply, a first wiring terminal (3) is grounded, and a third wiring terminal (7) and a fourth wiring terminal (8) are both connected with a voltage maintaining power supply and used for separating sample components;
step 2: after the separation is finished, the first binding post (3) is connected with a voltage maintaining power supply, and the third binding post (7) is grounded and used for transferring and collecting a group of sample components after the components are separated; and connecting the terminal III (7) with a maintaining voltage, and connecting the terminal IV (8) with the ground for transferring and collecting another group of sample components after the separated components are collected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110204040.8A CN112963984A (en) | 2021-02-24 | 2021-02-24 | Multichannel refrigerating device for microtube electrophoresis separation system and working method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110204040.8A CN112963984A (en) | 2021-02-24 | 2021-02-24 | Multichannel refrigerating device for microtube electrophoresis separation system and working method thereof |
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| CN112963984A true CN112963984A (en) | 2021-06-15 |
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| CN202110204040.8A Pending CN112963984A (en) | 2021-02-24 | 2021-02-24 | Multichannel refrigerating device for microtube electrophoresis separation system and working method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW522229B (en) * | 2002-04-04 | 2003-03-01 | Ind Tech Res Inst | Electrophoresis apparatus and its used hand-held detector. |
| TWI247891B (en) * | 2001-09-28 | 2006-01-21 | Univ Nat Cheng Kung | Sample analysis system using micro fluid module |
| TWI274040B (en) * | 2005-12-23 | 2007-02-21 | Ind Tech Res Inst | Microfluidic device and method of manufacturing the same |
| TWI285264B (en) * | 2000-11-29 | 2007-08-11 | Japan Science & Tech Corp | Nucleic acid analyzing method |
| CN206121523U (en) * | 2016-09-07 | 2017-04-26 | 西京学院 | Multichannel plastics chip device |
-
2021
- 2021-02-24 CN CN202110204040.8A patent/CN112963984A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI285264B (en) * | 2000-11-29 | 2007-08-11 | Japan Science & Tech Corp | Nucleic acid analyzing method |
| TWI247891B (en) * | 2001-09-28 | 2006-01-21 | Univ Nat Cheng Kung | Sample analysis system using micro fluid module |
| TW522229B (en) * | 2002-04-04 | 2003-03-01 | Ind Tech Res Inst | Electrophoresis apparatus and its used hand-held detector. |
| TWI274040B (en) * | 2005-12-23 | 2007-02-21 | Ind Tech Res Inst | Microfluidic device and method of manufacturing the same |
| CN206121523U (en) * | 2016-09-07 | 2017-04-26 | 西京学院 | Multichannel plastics chip device |
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Application publication date: 20210615 |
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