CN112961763B - Sampling device for periodic detection of microorganisms - Google Patents
Sampling device for periodic detection of microorganisms Download PDFInfo
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- CN112961763B CN112961763B CN202110161993.0A CN202110161993A CN112961763B CN 112961763 B CN112961763 B CN 112961763B CN 202110161993 A CN202110161993 A CN 202110161993A CN 112961763 B CN112961763 B CN 112961763B
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- bag
- push rod
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- 238000005070 sampling Methods 0.000 title claims abstract description 114
- 244000005700 microbiome Species 0.000 title claims abstract description 57
- 238000001514 detection method Methods 0.000 title claims abstract description 24
- 230000000737 periodic effect Effects 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 59
- 238000002347 injection Methods 0.000 claims abstract description 57
- 239000007924 injection Substances 0.000 claims abstract description 57
- 238000001125 extrusion Methods 0.000 claims abstract description 23
- 238000000429 assembly Methods 0.000 claims abstract description 8
- 230000000712 assembly Effects 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 8
- 238000003825 pressing Methods 0.000 claims description 19
- 238000000926 separation method Methods 0.000 claims description 11
- 238000003860 storage Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 10
- 238000005406 washing Methods 0.000 abstract description 4
- 238000009434 installation Methods 0.000 description 12
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- -1 cannot be reused Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of biological experimental equipment, and particularly relates to a sampling device for periodic detection of microorganisms. Comprising the following steps: the injection tube is prepared from washable materials; the needle head is communicated with the head end of the injection tube and can be detachably arranged at the head end of the injection tube; the push rod is arranged in the injection tube; the side wall of the injection tube is provided with a liquid separating hole close to the needle head, and the liquid separating hole is detachably sleeved with a sampling bag; an extrusion assembly comprising a platen, a handle, and a movable bar; the number of squeeze assemblies is at least two, which are evenly distributed around the outside of the syringe, and one of the squeeze assemblies' pressure plates is capable of contacting the sampling bag. The whole sampling process of the device does not need ear washing balls, the taken microorganism sample liquid is directly stored in the sampling bag, the transfer is not needed again, and the risk of microorganism sample liquid pollution is reduced.
Description
Technical Field
The invention belongs to the technical field of biological experimental equipment, and particularly relates to a sampling device for periodic detection of microorganisms.
Background
In the process of culturing the microorganism liquid, the microorganism liquid needs to be sampled periodically, and the concentration of the microorganism or target substances in the culture liquid under different culture time is detected. Since the microorganism is sampled and then cultured, the sampling process is required to be performed in a sterile environment near the flame of the alcohol burner as short as possible in order to prevent pollution. The sampling devices of the prior art are pipettes or syringes. When the liquid is sucked by the pipette, an operator needs to hold the ear ball by one hand, holds the pipette by one hand, and also needs to hold a test tube for microorganism sample liquid, in order to ensure that a culture bottle filled with microorganism culture liquid and the taken microorganism sample liquid are not polluted, all instruments should be close to the flame of the alcohol lamp, however, the flame range of the alcohol lamp is limited, if all instruments are close to the flame, the operation environment is crowded, a spherical air bag of the ear ball is a blind end, the only opening is a top tubular air nozzle, and residual secretion in the air bag and the tubular air nozzle is not easy to thoroughly clean and sterilize, so that the potential danger of polluting the microorganism sample is formed. When the injector absorbs liquid, the use amount of the injector is large, the recovery is difficult in the medical field without the help of the ear washing ball, and the injector is possibly infected due to different physique of different patients, so that the medical acceptance requirement is to adopt a disposable injector in order to prevent infectious diseases. However, the syringe devices used in the field of microbiological experiments are less expensive and less stringent than those used in the medical field, so that aspiration of microbiological samples using a recyclable instrument, such as the pipettes described above, is possible. However, the syringes in the prior art are all made of disposable plastic materials, cannot be reused, and waste of resources is caused.
For the above reasons, there is a need to develop a reusable and ear-ball-independent microbiological sampling apparatus.
Disclosure of Invention
In order to solve the technical problems, the invention provides the sampling device for periodic detection of microorganisms, ear washing balls are not needed in the whole sampling process, and the taken microorganism sample liquid is directly stored in the sampling bag without transferring again, so that the risk of microorganism sample liquid pollution is reduced.
The invention aims to provide a sampling device for periodic detection of microorganisms, which comprises:
the injection tube is prepared from washable materials;
the needle head is communicated with the head end of the injection tube and can be detachably arranged at the head end of the injection tube;
the push rod is arranged in the injection tube;
the side wall of the injection tube is provided with a liquid separating hole close to the needle head, and the liquid separating hole is detachably sleeved with the sampling bag;
the extrusion assembly comprises a pressing plate, a handle and a movable rod, one end of the pressing plate is rotationally connected with the outer wall of the injection tube, the connecting part is positioned between the sampling bag and the needle head, the other end of the pressing plate is connected with one end of the handle, the other end of the handle is rotationally connected with one end of the movable rod, and the other end of the movable rod is rotationally connected with the tail end of the push rod; the number of squeeze assemblies is at least two, distributed around the outside of the syringe, and one of the squeeze assemblies' pressure plates is capable of contacting the sampling bag.
Preferably, the sampling device for periodic detection of microorganisms further comprises a spring, wherein one end of the spring is connected with the pressing plate or the handle or the junction of the pressing plate and the handle, and the other end of the spring is connected with the injection tube; and in the natural extension state of the spring, the sampling bag is naturally extended.
Preferably, the sampling device for periodic detection of microorganisms further comprises a guide plate, wherein an included angle between the guide plate and a plane where the tail end of the push rod is located is 0-60 degrees.
Preferably, the sampling device for periodic detection of microorganisms is characterized in that the sampling bag is made of transparent or semitransparent elastic capsule material capable of autonomously recovering shape.
Preferably, the sampling device for periodic detection of microorganisms further comprises an inclined elastic interception surface arranged in the injection tube, the elastic interception surface divides the interior of the injection tube into a liquid storage area at the head end and a push rod active area at the tail end, the liquid separation hole is positioned in the liquid storage area, and the tail end edge of the elastic interception surface is connected with the edge of the liquid separation hole, which is close to the tail end of the injection tube.
Preferably, the sampling device for periodic detection of microorganisms further comprises:
a first magnetic member provided on the sampling bag at a position for contact with the pressure plate and the syringe;
and the second magnetic component is arranged on the pressing plate and the injection tube and is matched and attracted with the first magnetic component.
Preferably, the sampling device for periodic detection of microorganisms further comprises a connecting pipe, wherein the connecting pipe is arranged on the liquid separation hole of the injection pipe, the bag opening of the sampling bag is elastic, the diameter of the bag opening when the sampling bag is opened naturally is smaller than that of the connecting pipe, and the bag opening of the sampling bag is sleeved on the connecting pipe.
Preferably, in the sampling device for periodic detection of microorganisms, a sealing ring for preventing liquid leakage is further provided at the edge of the sampling bag.
Preferably, the sampling device for periodic detection of microorganisms further comprises:
the head end mounting sleeve is sleeved outside the injection tube head end, and one end of the pressing plate is rotationally connected to the head end mounting sleeve and is not mounted on the injection tube;
the tail end installation sleeve is sleeved at the tail end of the push rod, and the other end of the pressing plate is rotationally connected to the tail end installation sleeve but not installed on the push rod.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the extrusion assembly is arranged, the sampling bag is extruded when the push rod is pushed into the injection tube during extrusion, when the push rod is pulled out of the injection tube during reverse expansion, the microorganism sample liquid enters the injection tube and the sampling bag, then the injection tube is rotated to be positioned above the sampling bag, the microorganism sample liquid in the injection tube completely flows into the sampling bag, and after sampling is finished, the sampling bag is disassembled and collected. The rest parts of the sampling device can be recovered and cleaned to wait for reuse. The whole sampling process does not need ear washing balls, and the taken microorganism sample liquid is directly stored in the sampling bag without transferring again, so that the residence time of the microorganism sample liquid in the air is reduced, and the risk of microorganism sample liquid pollution is greatly reduced. The operator holds the culture article or the culture bottle that are equipped with microorganism sample liquid with one's hand, and another hand holds sampling device, alone can accomplish the sample operation.
2. According to the invention, by arranging the spring, the microorganism sampling time is prevented from being overlong, the discomfort of reverse force application of fingers of an operator is reduced, the working efficiency of the extrusion assembly is higher, and the overall sampling efficiency is improved.
3. According to the invention, the head end mounting sleeve and the tail end mounting sleeve are arranged, and the head end mounting sleeve, the tail end mounting sleeve and the sampling bag are removed after sampling is finished, and because the head end mounting sleeve, the tail end mounting sleeve and the extrusion assembly are not contacted with microorganism sample liquid in the sampling process, the head end mounting sleeve, the tail end mounting sleeve and the extrusion assembly can be matched with the next group of injection tubes and push rods for use under simple disinfection, an operator only needs to prepare a sufficient number of injection tubes and push rods according to experimental requirements, and only one group of head end mounting sleeve, one group of tail end mounting sleeve and one group of extrusion assembly are needed.
Drawings
FIG. 1 is a schematic view showing the structure of a squeeze assembly of a sampling device for periodic detection of microorganisms according to example 1 of the present invention when the squeeze assembly is opened;
FIG. 2 is a schematic view showing the structure of a sampling device for periodic microorganism detection according to example 1 of the present invention when the sampling device is pressed by a pressing member
Fig. 3 is an enlarged view of the portion a of fig. 1 in accordance with the present invention.
FIG. 4 is a schematic diagram showing the connection of the sampling bag and the syringe according to embodiment 1 of the present invention;
fig. 5 is a schematic structural diagram of embodiment 2 of the present invention.
Description of the embodiments
In order that those skilled in the art will better understand the technical scheme of the present invention, the present invention will be further described with reference to specific embodiments and drawings.
In the description of the present invention, it should be understood that terms such as "head", "tail", and the like, respectively, correspond to the "upper", "lower" orientation shown in fig. 1, and that the orientation or positional relationship indicated by the terms such as "inner", "outer", and the like, is based on the orientation or positional relationship shown in fig. 1, only for convenience in describing the present invention and simplifying the description, and does not indicate or imply that the apparatus or element in question must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature.
Examples
The embodiment provides a sampling device for periodic detection of microorganisms, including syringe 1, the head end intercommunication of syringe 1 is equipped with detachable syringe needle 2, syringe 1 adopts steel pipe, ceramic tube or transparent glass tube preparation, syringe 2 is used for with microorganism appearance liquid direct contact, be equipped with push rod 3 in the syringe 1, promote push rod 3 to syringe 2's direction, then discharge syringe 1 internal air, then to syringe 1 outside pull push rod 3, then microorganism appearance liquid gets into syringe 1 inside, liquid dividing hole 11 has been seted up to the 1-4 centimetres department of syringe 1 lateral wall near syringe needle 2 tail end, can dismantle on the liquid dividing hole 11 and cup joint sampling bag 4, sampling bag 4 is located syringe 1 outside, syringe 1's outside is provided with extrusion assembly. The main function of the extrusion component is that the extrusion component extrudes the sampling bag 4 when pushing the push rod 3 into the injection tube 1 during extrusion, and the microorganism sample liquid enters the injection tube 1 and the sampling bag 4 when extracting the push rod 3 from the outside of the injection tube 1 during reverse expansion. Then the position of the injection tube 1 is rotated to be positioned above the sampling bag 4, and the microorganism sample liquid in the injection tube 1 completely flows into the sampling bag 4, and after the sampling is finished, the sampling bag 4 is disassembled and collected. The rest parts of the sampling device can be recovered and cleaned to wait for reuse. The whole sampling process does not need to wash the ear ball, and this embodiment has set up sampling bag 4, and the microorganism sample liquid of taking out is direct to be deposited in sampling bag 4, need not to shift once more, reduces its time of stay in the air, greatly reduced the risk that microorganism sample liquid pollutes.
The structure of the extrusion assembly is as follows: including clamp plate 5, handle 6 and movable rod 7, clamp plate 5 one end is rotated through hinge or pivot with syringe 1 outer wall and is connected, and the junction is located between sample bag 4 and the syringe needle 2 for clamp plate 5 can be to being close to or keep away from the direction removal of syringe 1, the one end fixed connection of clamp plate 5 other end and handle 6, the other end of handle 6 is rotated through hinge or pivot and is connected with the one end of movable rod 7, the other end of movable rod 7 is rotated through hinge or pivot and is connected with the tail end of push rod 3. The number of squeeze assemblies is at least two, which are distributed around the outside of the syringe 1, preferably evenly, and the pressure plate 5 of one of the squeeze assemblies is capable of contacting the sampling bag 4.
In the following, the working principle is described by taking two extrusion components as examples, before sampling, referring to fig. 1, when the push rod 3 is not completely pushed into the injection tube 1, the sampling bag 4 is between the head end of the push rod 3 and the head end of the injection tube 1, the sampling bag 4 is in a natural unfolding state, air exists in the sampling bag, two pressing plates 5, two handles 6 and two movable rods 7 are respectively located at positions far away from the injection tube 1, and the movable rods 7 and the tail end of the push rod 3 are in a coaxial distribution state. Referring to fig. 2, before sampling, the operator holds the handles 6 by hand, can refer to the manner of holding scissors to hold the handles 6, then the fingers exert force towards the direction of the syringe 1, so that the positions of the two handles 6 are gradually close, the positions of the two pressing plates 5 are also gradually close, the sampling bag 4 is gradually extruded, and the internal air is discharged through the syringe 1 and the needle 2. Simultaneously, because this application has set up movable rod 7, when the in-process that two handles 6 are close to each other, the angle of movable rod 7 changes, then push rod 3 pushes gradually in syringe 1 in order to adapt to the angle change of movable rod 7, along with push rod 3 pushes into syringe 1 in, the intraductal air of syringe 1 is discharged through syringe needle 2, and the extrusion subassembly is in extrusion state this moment. Then, an operator holds a culture or a culture bottle filled with the microorganism sample liquid by one hand, holds a sampling device by the other hand, inserts the needle head 2 into the microorganism sample liquid, and makes fingers holding the sampling device exert force reversely to enable the two pressing plates 5 and the two handles 6 to be gradually far away from the injection tube 1, and the movable rod 7 is restored to a state of being coaxial with the tail end of the push rod 3, so that the extrusion assembly is in an unfolding state. The tail end of the handle 6 is also provided with a guide plate 61 which forms an angle of 0-60 degrees, preferably 0 degrees, with the plane of the tail end of the push rod 3, so as to prevent the movable rod 7 from rotating in disorder.
In order to prevent the microorganism from sampling too long, in order to reduce the discomfort of reverse exertion of the fingers of an operator, in order to make the working efficiency of the extrusion assembly higher, in order to improve the overall sampling efficiency, the extrusion assembly further comprises a spring 8, one end of the spring 8 is fixedly connected with the pressing plate 5, or one end of the spring 8 is fixedly connected with the handle 6, or the junction of the pressing plate 5 and the handle 6 at one end of the spring 8, and the other end of the spring is fixedly connected with the injection tube 1. In the natural extension state of the spring 8, the head end of the push rod 3 is positioned near the tail end of the injection tube 1, and the sampling bag 4 is naturally extended. The springs 8 are gradually compressed when the two pressure plates 5 are pressed towards each other, and when the sampling is finished, the hands of the operator are relaxed, the opposite force is not needed, and the assembly can be pressed to be separated by the elasticity of the springs 8.
In order to allow faster aspiration of the microbial sample into the sampling bag 4 and to increase the rate of aspiration of the microbial sample, the sampling bag 4 is made of a transparent or translucent elastomeric bladder material that is capable of self-recovery of shape. When the pressure plate 5 extrudes the sample bag, the air in the sample bag 4 is discharged, when the pressure plate 5 does not extrude the sample bag, the sample bag 4 automatically recovers, and the suction force can improve the speed of the microorganism sample liquid entering the sample bag during sampling because the automatic recovery state needs to suck air or the microorganism sample liquid. In order to make the sucked microorganism sample liquid quickly enter the sampling bag 4, an inclined elastic interception surface 12 is arranged in the injection tube 1, the elastic interception surface 12 is made of materials such as an elastic film and a latex film, the elastic interception surface 12 divides the interior of the injection tube 1 into a liquid storage area at the head end and a push rod movable area at the tail end, a liquid separation hole 11 is arranged in the liquid storage area, and the tail end edge of the elastic interception surface 12 is connected with the edge of the liquid separation hole 11, which is close to the tail end of the injection tube 1.
Referring to fig. 3, in order to improve the contact area and the contact stability between the pressure plate 5 and the sampling bag 4 and between the sampling bag 4 and the syringe 1, the positions of the sampling bag 4 for contacting the pressure plate 5 and the syringe 1 are provided with first magnetic members 41, and the positions of the pressure plate 5 and the syringe 1 are provided with second magnetic members 51 matching and attracting the first magnetic members 41. The first magnetic member 41 and the second magnetic member 51 are both magnets; alternatively, the first magnetic member 41 may be a magnet or an iron piece, and the second magnetic member 51 may be a magnet.
Referring to fig. 4, in order to improve the mounting stability of the sampling bag 4 on the syringe 1, a connecting pipe 13 is arranged on the liquid separation hole 11 of the syringe 1, the connecting pipe 13 and the liquid separation hole 11 are integrally formed, or the joint between the edge of the connecting pipe 13 and the edge of the liquid separation hole 11 is sealed. The mouth of the sampling bag 4 is sleeved on the connecting pipe 13. The opening of the sampling bag 4 has elasticity, and the diameter of the opening is smaller than that of the connecting pipe 13 when the sampling bag is opened naturally, so that the sealing performance of the connecting part of the sampling bag 4 and the connecting pipe 13 is strong after the sampling bag 4 is sleeved on the connecting pipe 13, and the edge of the sampling bag 4 is also provided with a sealing ring 42 for placing liquid leakage.
Examples
The sampling device for periodic detection of microorganisms is basically the same as that of embodiment 1, except that referring to fig. 5, in order to reduce the work of cleaning the instruments and improve the utilization rate of the instruments, the invention further comprises a head end mounting sleeve 9 and a tail end mounting sleeve 91, wherein the inner wall of the head end mounting sleeve 9 is sleeved, clamped or screwed outside the head end of the injection tube 1, one end of the pressure plate 5 is rotatably connected to the head end mounting sleeve 9 and is not mounted on the injection tube 1, the tail end mounting sleeve 91 is sleeved and clamped at the tail end of the push rod 3, and the other end of the pressure plate 5 is rotatably connected to the tail end mounting sleeve 91 and is not mounted on the push rod 3. Before sampling, the head end installation sleeve 9 and the tail end installation sleeve 91 are installed at corresponding positions according to the structure shown in fig. 5, then sampling is performed according to the working principle of embodiment 1, after sampling is finished, the head end installation sleeve 9, the tail end installation sleeve 91 and the sampling bag 4 are removed, and in the sampling process, the head end installation sleeve 9, the tail end installation sleeve 91 and the extrusion assembly are not contacted with microorganism sample liquid, so that the head end installation sleeve 9, the tail end installation sleeve 91 and the extrusion assembly can be matched with the next group of injection tubes 1 and push rods 3 for use under simple disinfection, and operators only need to prepare a sufficient number of injection tubes 1 and push rods 3 according to experimental requirements, and only need to use one group of head end installation sleeve 9, tail end installation sleeve 91 and extrusion assembly.
It should be noted that, the connection relationships of the components not specifically mentioned in the present invention are all default to the prior art, and the connection relationships of the structures are not described in detail because they do not relate to the invention points and are common applications of the prior art.
It should be noted that, when numerical ranges are referred to in the present invention, it should be understood that two endpoints of each numerical range and any numerical value between the two endpoints are optional, and because the adopted step method is the same as the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (6)
1. A sampling device for periodic detection of microorganisms, comprising:
an injection tube (1) which is prepared from a washable material;
the needle head (2) is communicated with and can be detachably arranged at the head end of the injection tube (1);
a push rod (3) arranged in the injection tube (1);
the sampling bag (4) is arranged on the side wall of the injection tube (1) close to the needle head (2), a liquid separating hole (11) is formed in the side wall of the injection tube (1), and the sampling bag (4) is detachably sleeved on the liquid separating hole (11); the sampling bag (4) is made of transparent or semitransparent elastic bag body material capable of automatically recovering the shape;
the extrusion assembly comprises a pressing plate (5), a handle (6) and a movable rod (7), one end of the pressing plate (5) is rotationally connected with the outer wall of the injection tube (1), a connecting part is positioned between the sampling bag (4) and the needle head (2), the other end of the pressing plate (5) is connected with one end of the handle (6), the other end of the handle (6) is rotationally connected with one end of the movable rod (7), and the other end of the movable rod (7) is rotationally connected with the tail end of the push rod (3); the number of said squeeze assemblies is at least two, distributed around the outside of said syringe (1), and wherein the pressure plate (5) of one of said squeeze assemblies is capable of contacting said sampling bag (4);
the elastic retaining surface (12) is arranged in the injection tube (1), the elastic retaining surface (12) divides the interior of the injection tube (1) into a liquid storage area at the head end and a push rod active area at the tail end, the liquid separation hole (11) is positioned in the liquid storage area, and the tail end edge of the elastic retaining surface (12) is connected with the edge of the liquid separation hole (11) close to the tail end of the injection tube (1);
a first magnetic member (41) provided on the sampling bag (4) at a position for contact with the pressure plate (5) and the syringe (1);
and a second magnetic member (51) provided on the pressure plate (5) and the injection tube (1) and attracted in a matching manner to the first magnetic member (41).
2. The sampling device for the periodic detection of microorganisms according to claim 1, characterized in that it further comprises a spring (8), one end of said spring (8) being connected to the junction of said pressure plate (5) or said handle (6) or both, the other end being connected to said syringe (1); in the natural extension state of the spring (8), the sampling bag (4) is naturally extended.
3. The sampling device for periodic detection of microorganisms according to claim 2, further comprising a guide plate (61) having an angle of 0-60 degrees with respect to the plane in which the trailing end of the push rod (3) is located.
4. The sampling device for periodic detection of microorganisms according to claim 1, further comprising a connecting tube (13) provided on the liquid separation hole (11) of the injection tube (1), wherein the opening of the sampling bag (4) has elasticity, the opening diameter of which is smaller than the diameter of the connecting tube (13) when naturally opened, and the opening of the sampling bag (4) is sleeved on the connecting tube (13).
5. The sampling device for periodic detection of microorganisms according to claim 4, wherein the edges of the sampling bag (4) are further provided with a sealing ring (42) for preventing leakage of liquid.
6. The sampling device for periodic detection of microorganisms according to claim 1, further comprising:
the head end mounting sleeve (9) is sleeved outside the head end of the injection tube (1), and one end of the pressing plate (5) is rotationally connected to the head end mounting sleeve (9) and is not mounted on the injection tube (1);
the tail end mounting sleeve (91) is sleeved at the tail end of the push rod (3), and the other end of the pressing plate (5) is rotationally connected to the tail end mounting sleeve (91) but not mounted on the push rod (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110161993.0A CN112961763B (en) | 2021-02-05 | 2021-02-05 | Sampling device for periodic detection of microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110161993.0A CN112961763B (en) | 2021-02-05 | 2021-02-05 | Sampling device for periodic detection of microorganisms |
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| Publication Number | Publication Date |
|---|---|
| CN112961763A CN112961763A (en) | 2021-06-15 |
| CN112961763B true CN112961763B (en) | 2024-01-09 |
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| CN202110161993.0A Active CN112961763B (en) | 2021-02-05 | 2021-02-05 | Sampling device for periodic detection of microorganisms |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3875012A (en) * | 1974-01-30 | 1975-04-01 | Wadley Res Inst & Blood Bank | Apparatus and method for the detection of microbial pathogens |
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