CN112957324A - Preparation method and application of pickering emulsion by using prolamin to load eucommia chlorogenic acid - Google Patents
Preparation method and application of pickering emulsion by using prolamin to load eucommia chlorogenic acid Download PDFInfo
- Publication number
- CN112957324A CN112957324A CN202110172386.4A CN202110172386A CN112957324A CN 112957324 A CN112957324 A CN 112957324A CN 202110172386 A CN202110172386 A CN 202110172386A CN 112957324 A CN112957324 A CN 112957324A
- Authority
- CN
- China
- Prior art keywords
- chlorogenic acid
- prolamin
- pickering emulsion
- load
- oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229940074393 chlorogenic acid Drugs 0.000 title claims abstract description 43
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- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 title claims abstract description 42
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 title claims abstract description 42
- 235000001368 chlorogenic acid Nutrition 0.000 title claims abstract description 42
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Abstract
The invention discloses a method for preparing pickering emulsion by using prolamin loaded eucommia ulmoides chlorogenic acid, which utilizes the unique dissolubility and self-assembly characteristic of the prolamin and adopts an anti-solvent method to prepare composite nano particles of the prolamin loaded chlorogenic acid, so as to further prepare pickering emulsion, and has the advantages of simple operation, less emulsifier dosage, lower cost, environmental friendliness, uniform particle size distribution of the prepared pickering emulsion, no surfactant, low toxicity and obvious bioactivity, and because the alcohol-soluble nano-particles form a compact interface layer at the oil-water interface, the alcohol-soluble nano-particles have higher stability and biocompatibility, and is degradable, and the polysaccharide-based particles have stronger spatial stability, can resist flocculation and coagulation phenomena, thereby further improving the stability of the Pickering emulsion, obviously improving the solubility and stability of the chlorogenic acid and improving the bioavailability of the chlorogenic acid.
Description
The technical field is as follows:
the invention relates to the technical field of food, medicine and cosmetics, and particularly relates to a preparation method and application of a pickering emulsion by using prolamin to load eucommia chlorogenic acid.
Background art:
eucommia Chlorogenic Acid (CA) is depside generated from caffeic acid and quinic acid, is a phenylpropanoid compound generated by a shikimic acid pathway in an aerobic respiration process of a plant body, has good biological activity, but has poor solubility and stability, so that the bioavailability of the eucommia Chlorogenic acid is greatly influenced. At present, the bioavailability of the eucommia chlorogenic acid can be improved by constructing a safe and stable embedding system through an emulsification technology. The emulsion prepared conventionally is an unstable system due to large oil-water interfacial area, and generally a small molecular surfactant or a high molecular polymer is added as an emulsifier or other stabilizer with small molecular weight, so that the interfacial tension can be reduced, the steric hindrance and the electrostatic repulsion between liquid drops can be increased, and a uniform system is formed, thereby achieving the stable effect. However, in practice, the conventional emulsifiers have poor biocompatibility, are not degradable, and may cause tissue inflammation or cell damage, so that an excessive amount of the emulsifiers must be removed from the sample, which also affects the application range of the emulsion. Pickering (Pickering) emulsions are emulsion systems stabilized by solid particles instead of surfactants. Compared to conventional emulsions, Pickering emulsions have advantages including: the use of a surfactant can be reduced or avoided, and the cost is reduced; the safety is high, and the harm to human bodies and the environment is reduced; the adhesion of solid particles on an oil-water interface is irreversible, so that liquid drops are effectively prevented from being aggregated, and the stability is high; the solid particles have stronger adhesion on the cell surface, can be used for embedding and transmitting functional factors, and have slow release and targeting effects; and the influence of the external environment is not easy to be caused, and the particle size of the particles is controllable. Therefore, the Pickering emulsion has important application value in the food field. The current use of solid particles for stabilizing Pickering emulsions is mainly focused on inorganic and synthetic particles. This also limits the use of Pickering in the food, pharmaceutical and cosmetic industries. Therefore, the development of environmentally friendly, renewable and degradable nanoparticle colloidal particles for stabilizing Pickering emulsion has become a research hotspot at present.
Zein (Zein) is a natural edible plant macromolecule, is a main storage protein existing in corn, accounts for about 80% of total protein of the corn, contains a large amount of hydrophobic amino acids in a molecular structure, has amphipathy, is good in biocompatibility, and has a unique self-assembly characteristic, so that the Zein is widely applied. Therefore, the zein can be used as a stabilizer of Pickering emulsion, is renewable and nontoxic, has good biodegradability, and can be widely applied to the industries of food, medicine and cosmetics. However, Pickering emulsions prepared from a single prolamin are unstable and exhibit a delamination phenomenon, so that the application of Pickering emulsions in complex food systems is also greatly limited. At present, research shows that after the prolamin is completely dissolved in the ethanol water solution, the prolamin forms nano-particles in the water phase by an anti-solvent precipitation method, and the nano-particles can be used for stabilizing Pickering emulsion. However, based on the particularity of the mixed system, the stability of the emulsion still needs to be further improved by adding the stabilizer dropwise into the composite system when the composite nano particles are prepared by the anti-solvent method. Therefore, the focus of attention has been on how to prepare pickering emulsions that are both safe and stable from natural plant materials.
The invention content is as follows:
the invention aims to provide a preparation method and application of pickering emulsion by using prolamin loaded eucommia ulmoides chlorogenic acid, which are characterized in that unique solubility and self-assembly characteristics of prolamin are utilized, a desolvation method is adopted to prepare composite nano particles of prolamin loaded chlorogenic acid, and pickering emulsion loaded with chlorogenic acid is further prepared.
The invention is realized by the following technical scheme:
a preparation method of Pickering emulsion by using prolamin to load eucommia chlorogenic acid comprises the following steps: dissolving alcohol soluble protein and chlorogenic acid into an ethanol solution together, wherein the mass ratio of the alcohol soluble protein to the chlorogenic acid is 10-50: 1, the material-liquid ratio of the alcohol soluble protein to the ethanol solution is 0.1-2.0 g: 20mL, adding polysaccharide-based particles as a stabilizer solution, the volume ratio of the stabilizer solution to the alcohol soluble protein is 2: 1-10: 1, adjusting the pH of the solution to 5.0 by 1.0-2.0mol/L NaOH, stirring at normal temperature for 0.5-3 h, removing ethanol by reduced pressure concentration, centrifuging to remove large particles, obtaining an alcohol soluble protein suspension loaded with the chlorogenic acid, adding an oil phase, the volume content of the oil phase is 20-80% (V/V), mixing uniformly, and shearing at high speed to obtain an alcohol soluble protein loaded chlorogenic acid pickering emulsion.
Preferably, the prolamin is zein or gliadin.
Preferably, the volume fraction of the ethanol solution is 60-80%.
Preferably, the polysaccharide-based particles are selected from gum arabic, pectin, chitosan, cellulose, guar gum, sodium alginate, carrageenan, and the like.
Preferably, the stirring speed is 1000-2000 rpm.
Preferably, the temperature of the reduced pressure concentration is 40-60 ℃, rotary evaporation is adopted, the rotating speed is 200-800 r/min, and the pressure is-0.1 MPa.
Preferably, the oil phase is edible oil or medium-chain fatty acid ester, including vegetable oil such as eucommia seed oil, corn oil, peanut oil, soybean oil, olive oil, etc.
Preferably, the shear rate is 8000-12000 rpm.
Preferably, the mass fraction of polysaccharide-based particles of the stabilizer solution is between 0.25% and 10%.
Compared with the prior art, the invention has the beneficial effects that:
(1) the unique solubility and self-assembly characteristics of the alcohol soluble protein are utilized, the composite nano-particles of the alcohol soluble protein loaded with the chlorogenic acid are prepared by an anti-solvent method, and the Pickering emulsion loaded with the chlorogenic acid is further prepared.
(2) The polysaccharide-based particles are selected from Arabic gum, pectin, chitosan, cellulose, guar gum, sodium alginate and carrageenan, and have better emulsifying property and surface activity which are easily adjusted by modification, stronger space stability and flocculation and agglomeration resistance, so the stability of the Pickering emulsion can be further improved. The preparation method not only enables the Pickering emulsion to have better stability, but also can effectively improve the stability and solubility of the chlorogenic acid as an active ingredient and improve the bioavailability of the chlorogenic acid.
(3) The raw materials used in the invention are all selected from natural plant sources, are healthy and safe, and have wide sources. Has wide application prospect in the food and health care product industry.
Description of the drawings:
FIG. 1 shows the particle size and dispersion coefficient of different composite nanoparticles.
Figure 2 is the bioavailability of different samples.
The specific implementation mode is as follows:
the following is a further description of the invention and is not intended to be limiting. Any changes, simplifications or improvements made in accordance with the description of the present invention shall fall within the protection scope of the present invention.
Example 1: the zein-chlorogenic acid composite particles are prepared by an anti-solvent method.
2.5g of zein is accurately weighed and dispersed into 100mL of 70% (v/v) ethanol solution, and the mixture is fully stirred for 2 hours at room temperature to ensure that the zein is fully dissolved. Weighing 100mg of chlorogenic acid, fully dissolving in 70% ethanol solution, and diluting to 50mL to obtain chlorogenic acid solution with concentration of 2.0 mg/mL. Adding a proper amount of zein solution into a chlorogenic acid solution according to a ratio, uniformly mixing, wherein the mass ratio of zein to chlorogenic acid is 10: 1, 20: 1, 30: 1, 40: 1 and 50: 1, stirring at 1500rpm for 1.0h at 25 ℃, adding a pectin (pectin) solution (1.0%) with pH of 4.0, the volume ratio of the zein to the solution is 3: 1, adjusting the pH of the solution to 5.0 by 1.0mol/L NaOH, continuously stirring for 1.0h, concentrating at 40 ℃ under reduced pressure, removing ethanol, centrifuging at 4000rpm for 15min, removing larger particle substances, and collecting supernatant to obtain the composite nanoparticle solution. And (3) mixing the composite nano-particle solution with eucommia seed oil (30%), stirring mechanically (10000r/min, 5min), and stirring uniformly to obtain a Pickering emulsion with prolamin-loaded chlorogenic acid, wherein the sample is stored at 4 ℃ for later use.
The pH was adjusted to 1.5 using 1.0mol/L HCl and 1.0mol/L NaOH solutions, and 1.0g pepsin was added to 100mL of the solution, which was filtered through a 0.22 μm filter to obtain simulated gastric fluid. Preheating simulated gastric juice to 37 ℃, weighing 20mg of different composite nanoparticle samples and chlorogenic acid samples, placing the samples in a test tube containing 10.0mL of simulated gastric juice, and oscillating in a water bath of 100r/min for 2.0h at 37 ℃ under the condition of keeping out of the sun to complete the simulated digestion process. After digestion with simulated gastric fluid, the pH was adjusted to 5.0 3.6mg/mL trypsin and 11.25mg/mL sodium cholate were dissolved in 0.1mol/LNa2CO3And (5) obtaining the simulated intestinal juice. The simulated intestinal fluid is preheated to 37 ℃, 5.0mL is added into the test tube, the pH is adjusted to 7.0, and the test tube is shaken in a water bath of 100r/min for 2.0h at 37 ℃ in a dark environment, so that the simulated digestion process is completed. After the whole digestion process is finished, 1.0mL of digestion dispersion is collected and centrifuged at 10000rpm for 15min at 4 ℃, and the supernatant is taken to detect the content of chlorogenic acid.
The above-described embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the person skilled in the art on the basis of the present invention is within the protection scope of the present invention. The protection scope of the invention is subject to the claims.
Claims (10)
1. A preparation method of Pickering emulsion by using prolamin to load eucommia chlorogenic acid is characterized by comprising the following steps: dissolving alcohol soluble protein and chlorogenic acid into an ethanol solution together, wherein the mass ratio of the alcohol soluble protein to the chlorogenic acid is 10-50: 1, the material-liquid ratio of the alcohol soluble protein to the ethanol solution is 0.1-2.0 g: 20mL, adding polysaccharide-based particles as a stabilizer solution, the volume ratio of the stabilizer solution to the alcohol soluble protein is 2: 1-10: 1, adjusting the pH of the solution to 5.0 by 1.0-2.0mol/L NaOH, stirring at normal temperature for 0.5-3 h, removing ethanol by concentration under reduced pressure, centrifuging to remove large particles, obtaining an alcohol soluble protein suspension loaded with the chlorogenic acid, adding an oil phase, wherein the volume content of the oil phase is 20-80%, mixing uniformly, and performing high-speed shearing to obtain an alcohol soluble protein loaded chlorogenic acid pickering emulsion.
2. The method of claim 1, wherein the prolamin is zein or gliadin.
3. The preparation method of a pickering emulsion using prolamin to load eucommia ulmoides chlorogenic acid according to claim 1 or 2, wherein the volume fraction of the ethanol solution is 60-80%.
4. A method of preparing a pickering emulsion using prolamin loaded eucommia ulmoides chlorogenic acid according to claim 1 or 2, wherein the polysaccharide-based particles are selected from any one of acacia gum, pectin, chitosan, cellulose, guar gum, sodium alginate and carrageenan.
5. The preparation method of the pickering emulsion using prolamin to load eucommia ulmoides chlorogenic acid according to claim 1 or 2, wherein the stirring speed is 1000-2000 rpm.
6. The preparation method of pickering emulsion using prolamin to load eucommia ulmoides chlorogenic acid according to claim 1 or 2, wherein the temperature of reduced pressure concentration is 40-60 ℃, rotary evaporation is adopted, the rotation speed is 200-800 r/min, and the pressure is-0.1 Mpa.
7. The preparation method of pickering emulsion using prolamin to load eucommia ulmoides chlorogenic acid according to claim 1 or 2, wherein the oil phase is edible oil or medium-chain fatty acid ester.
8. The method of claim 7, wherein the vegetable oil is selected from the group consisting of eucommia seed oil, corn oil, peanut oil, soybean oil, and olive oil.
9. The preparation method of a pickering emulsion using prolamin to load eucommia ulmoides chlorogenic acid according to claim 1 or 2, wherein the shear rate is 8000-12000 rpm.
10. A method for preparing a pickering emulsion using prolamin to load eucommia ulmoides chlorogenic acid according to claim 1 or 2, wherein the mass fraction of polysaccharide-based particles in the stabilizer solution is 0.25% -10%.
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