CN110025002A - A kind of preparation method and application of alcohol soluble protein-polysaccharide composite particle - Google Patents
A kind of preparation method and application of alcohol soluble protein-polysaccharide composite particle Download PDFInfo
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- CN110025002A CN110025002A CN201910334243.1A CN201910334243A CN110025002A CN 110025002 A CN110025002 A CN 110025002A CN 201910334243 A CN201910334243 A CN 201910334243A CN 110025002 A CN110025002 A CN 110025002A
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- soluble protein
- zeins
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- composite particle
- alcohol soluble
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 79
- 239000011246 composite particle Substances 0.000 title claims abstract description 72
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 38
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 239000000770 propane-1,2-diol alginate Substances 0.000 claims abstract description 53
- 229920002494 Zein Polymers 0.000 claims abstract description 43
- 108010055615 Zein Proteins 0.000 claims abstract description 43
- 239000006210 lotion Substances 0.000 claims abstract description 41
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000006185 dispersion Substances 0.000 claims abstract description 27
- HDSBZMRLPLPFLQ-UHFFFAOYSA-N Propylene glycol alginate Chemical compound OC1C(O)C(OC)OC(C(O)=O)C1OC1C(O)C(O)C(C)C(C(=O)OCC(C)O)O1 HDSBZMRLPLPFLQ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 235000010409 propane-1,2-diol alginate Nutrition 0.000 claims abstract description 25
- 239000007787 solid Substances 0.000 claims abstract description 10
- 239000011259 mixed solution Substances 0.000 claims abstract description 9
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 7
- 238000005119 centrifugation Methods 0.000 claims abstract description 5
- 235000019441 ethanol Nutrition 0.000 claims description 55
- 239000003921 oil Substances 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 23
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 8
- 230000006837 decompression Effects 0.000 claims description 6
- 238000004945 emulsification Methods 0.000 claims description 6
- 229920000615 alginic acid Polymers 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- -1 Propylene glycol ester Chemical class 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 239000000783 alginic acid Substances 0.000 claims description 2
- 229960001126 alginic acid Drugs 0.000 claims description 2
- 150000004781 alginic acids Chemical class 0.000 claims description 2
- 239000008157 edible vegetable oil Substances 0.000 claims description 2
- 238000007710 freezing Methods 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 2
- 150000002711 medium chain fatty acid esters Chemical class 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 235000013376 functional food Nutrition 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 239000012071 phase Substances 0.000 description 31
- 235000019198 oils Nutrition 0.000 description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 25
- 239000002245 particle Substances 0.000 description 15
- 238000003756 stirring Methods 0.000 description 10
- 150000004804 polysaccharides Chemical class 0.000 description 6
- 238000010008 shearing Methods 0.000 description 5
- 239000003381 stabilizer Substances 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 238000013019 agitation Methods 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 238000001704 evaporation Methods 0.000 description 4
- 230000008020 evaporation Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 229940072056 alginate Drugs 0.000 description 3
- 125000005456 glyceride group Chemical group 0.000 description 3
- 238000003475 lamination Methods 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000012296 anti-solvent Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000001218 confocal laser scanning microscopy Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000001016 Ostwald ripening Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000000227 bioadhesive Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Cosmetics (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
The invention discloses a kind of alcohol soluble protein-polysaccharide composite particle preparation method and application for belonging to new material and technical field of functional food.Zeins ethanol water, propylene glycol alginate ethanol water are mixed to get mixed solution by the present invention in equal volume under stiring, the mixed solution obtains alcohol soluble protein-polysaccharide composite particle dispersion through vacuum rotary steam, centrifugation, and alcohol soluble protein-polysaccharide composite particle is made after dry；Preparation method is simple and easy to control；Obtained alcohol soluble protein-polysaccharide composite particle is used to prepare Pickering lotion as solid emulsifier, and the Pickering lotion of preparation has preferable thermal stability and storage-stable.
Description
Technical field
The invention belongs to new material and technical field of functional food, in particular to a kind of alcohol soluble protein-polysaccharide composite particle
Preparation method and application.
Background technique
Conventional emulsion is usually to reduce by two kinds of mutual not phases using Small molecular surfactant or high molecular polymer as emulsifier
The interfacial tension of solution body is widely used in the fields such as food, drug, petrochemical industry to form uniform system.
Pickering (pik woods) lotion is to replace surfactant and stable emulsion system by solid particle.With conventional emulsion phase
Than Pickering lotion has the advantage that the use for avoiding or reducing surfactant, reduces costs；Toxicity
It is small, it is highly-safe, reduce the harm to human body and environment；Adhesive attraction of the solid particle on oil-water interfaces is irreversible, tool
Have compared with stiff stability, not the influence vulnerable to external environment (pH, temperature, ionic strength etc.), grain diameter has controllability.Cause
This, Pickering lotion has important application value in field of food.But at present for stablizing Pickering lotion
The research of solid particle is concentrated mainly on inorganic particulate or compound particle, limits Pickering lotion in the food industry
Application.Therefore, environmentally friendly, renewable and biodegradable food-grade colloidal solid is developed for stablizing
Pickering lotion has become current research hotspot.
Zeins is major storage protein matter present in corn, contains a large amount of hydrophobicity ammonia in molecular structure
Base acid, makes it have amphipathic, has a unique self assembly characteristic, and have good biocompatibility and bioadhesive.
Therefore, zeins particle is used as the stabilizer of Pickering lotion.But single zeins preparation
Pickering lotion it is unstable, be in obvious lamination, be difficult to apply to food system.Existing research the result shows that, alcohol is molten
Proteolytic forms colloidal solid after ethanol water, using the anti-solvent precipitation method in water phase, then by non-covalent
Interaction generates composite colloid particle for stablizing Pickering lotion with hy-drophilic polysaccharide.But anti-solvent precipitates legal system
Zeins ethanol water need to be injected or be added dropwise to polysaccharide water by standby zeins-polysaccharide composite particle
In solution, procedure parameter is difficult to control, and concentration process time consumption and energy consumption.The current research master in relation to zeins and polysaccharide
Hy-drophilic polysaccharide is concentrated on, the research with amphiphilic polysaccharide interaction still belongs to blank.
Summary of the invention
The purpose of the present invention is to provide a kind of alcohol soluble protein-polysaccharide composite particle preparation method and application, specific skills
Art scheme is as follows:
A kind of preparation method of alcohol soluble protein-polysaccharide composite particle, which is characterized in that zeins ethyl alcohol is water-soluble
Liquid, propylene glycol alginate ethanol water are mixed to get mixed solution in equal volume under stiring, and the mixed solution is revolved through decompression
It steams, centrifugation obtains alcohol soluble protein-polysaccharide composite particle dispersion, obtained alcohol soluble protein-polysaccharide composite particle after dry；
The alcohol soluble protein-polysaccharide composite particle prepares Pickering lotion as solid emulsifier.
The zeins, propylene glycol alginate raw material are food-grade.
The zeins ethanol water is mixed to get with zeins and ethanol water, the alginic acid
Propylene glycol ester ethanol water is mixed to get with propylene glycol alginate and ethanol water；Every 1mL zeins ethanol water
Contain 0.1~50mg of zeins in solution.
The volume fraction of ethyl alcohol is 60%~90% in the ethanol water.
Zeins and propylene glycol alginate mass ratio are 40:1~1:2 in the mixed solution；The mixing is molten
The mixing time of liquid is 1~8h.
The temperature of the vacuum rotary steam is 35~65 DEG C, and rotation speed is 200~800r/min, and pressure is -0.1MPa；Institute
Stating centrifugal speed is 1500~6500rad/min, and centrifugation time is 10~60min.
Composite particles concentration is 0.25~10% in the alcohol soluble protein-polysaccharide composite particle dispersion.
The mode of the drying is vacuum freeze drying, and pre-freeze handles 1~12h, and pre-freezing temperature is -20~-50 DEG C, dry
Time is 1~12h.
Alcohol soluble protein-application of the polysaccharide composite particle in Pickering lotion of the preparation method preparation, the alcohol
Molten protein-PS composite particles prepare Pickering lotion as solid emulsifier, specifically: by the alcohol soluble protein-polysaccharide
Composite particles dispersion liquid is mixed with oil, and Pickering lotion is obtained after emulsification pretreatment.
The alcohol soluble protein-polysaccharide composite particle aqueous dispersion is that alcohol soluble protein-polysaccharide of preparation method preparation is multiple
Close particle dispersion, or the dispersion being dispersed in water for alcohol soluble protein-polysaccharide composite particle prepared by the preparation method
Liquid.
The oil is mutually edible oil and/or medium-chain fatty glyceride.
The volume ratio that the oil is mutually mixed with alcohol soluble protein-polysaccharide composite particle dispersion is 1:9~4:1；The shearing
Speed is 7000~18000rpm, and the time is 2~10min.
Composite particles concentration is 0.25~10% in the Pickering lotion.
The invention has the benefit that
(1) zeins and propylene glycol alginate are formed again by electrostatic, hydrophobic and hydrogen bond action in the present invention
Particle is closed, grain graininess uniformly in 200~600nm, has preferable wetability, and three-phase (water phase-oil phase-solid phase) contact angle exists
Between 80~95 degree；
(2) it is prepared using zeins of the present invention-propylene glycol alginate composite particles as solid emulsifier
Pickering lotion, zeins-propylene glycol alginate composite particles can form fine and close package in oil droplets
Layer, oil droplet size effectively prevent aggregation and the Ostwald ripening of oil droplet, the Pickering lotion tool of preparation uniformly at 10~120 μm
There are preferable thermal stability and storage-stable, and there is edibility, avoids harm of the conventional emulsifier to human health；
(3) zeins prepared by the present invention-propylene glycol alginate composite particles, raw material are food-grade, and having can
Feeding habits and from a wealth of sources, preparation method is simple and easy to control, and ethyl alcohol used is can be recycled in producing, green non-pollution, less energy consumption,
Processing cost is low, is suitble to industrialized production.
Detailed description of the invention
Attached drawing 1 is 1 zeins of embodiment-propylene glycol alginate composite particles SEM figure；
Attached drawing 2 is 2 zeins of embodiment-propylene glycol alginate three-phase contact angle figure；
Attached drawing 3 is 2 zeins of embodiment-propylene glycol alginate Pickering lotion CLSM figure.
Specific embodiment
The present invention provides a kind of alcohol soluble protein-polysaccharide composite particle preparation method and application, with reference to the accompanying drawing and
The present invention is described further for embodiment.
Embodiment 1
Alcohol soluble protein-polysaccharide composite particle is prepared as steps described below:
(1) 0.1g zeins is added in 50mL ethanol water (volume fraction of ethyl alcohol is 60%), room temperature
Stirring obtains zeins ethanol water to abundant dissolution；
(2) 0.005g propylene glycol alginate (zeins and propylene glycol alginate mass ratio be 20:1) is dispersed in
In 50mL ethanol water (volume fraction of ethyl alcohol is 60%), magnetic agitation obtains propylene glycol alginate second to being completely dissolved
Alcohol solution；
(3) zeins ethanol water obtained by step (1) is added to obtained by step (2) under stirring conditions
2h, which is stirred, in propylene glycol alginate ethanol water, after mixing obtains zeins-alginate propylene glycol ester admixture ethyl alcohol
Aqueous solution；
(4) composite particles are prepared using emulsification evaporation: using decompression Rotary Evaporators at 45 DEG C, 300r/min, pressure
For vacuum rotary steam under the conditions of -0.1MPa, ethyl alcohol and part water are removed；Then it is centrifuged 30min under 2000rpm revolving speed, to remove
The molten egg of corn alcohol is made after dry to get zeins-propylene glycol alginate composite particles dispersion liquid is arrived in larger particles
White-propylene glycol alginate composite particles.
Through in zeins obtained by the above method-propylene glycol alginate composite particles dispersion liquid composite particles it is dense
Degree is 1.5%, and the three-phase contact angle of particle is 85 °, grain diameter 200-300nm；The SEM figure of composite particles is specifically such as Fig. 1
It is shown, it will be seen from figure 1 that grain diameter is uniform, soilless sticking phenomenon.
Pickering lotion is prepared by stabilizer of gained zeins-propylene glycol alginate composite particles: by institute
Obtaining the dispersion liquid that zeins-propylene glycol alginate composite particles are dispersed in water will walk as water phase, or directly
Suddenly zeins-propylene glycol alginate composite particles dispersion liquid obtained by (4) is water phase；It is oil with medium-chain fatty glyceride
Oil is mutually added in water phase by phase, and oil phase volume score is 40% (v/v), and Pickering is made in shearing 3min under 8000rpm
Lotion.The oil droplet size of gained Pickering lotion is 20~60 μm, after 70 DEG C of heat treatment 30min, emulsion oil droplets partial size
Maintain 20~60 μm；After placing 30 days, do not occur lamination, there is preferable thermal stability and storage-stable.
Embodiment 2
Alcohol soluble protein-polysaccharide composite particle is prepared as steps described below:
(1) 0.3g zeins is added in 50mL ethanol water (volume fraction of ethyl alcohol is 70%), often
Temperature stirring obtains zeins ethanol water to being completely dispersed；
(2) 0.03g propylene glycol alginate (zeins and propylene glycol alginate mass ratio be 10:1) is added to
In 50mL ethanol water (volume fraction of ethyl alcohol is 70%), magnetic agitation obtains propylene glycol alginate second to being completely dissolved
Alcohol solution；
(3) zeins ethanol water obtained by step (1) is added to obtained by step (2) under stirring conditions
Propylene glycol alginate ethanol water stirs 3h and obtains zeins-alginate propylene glycol ester admixture ethanol water after mixing
Solution；
(4) composite particles are prepared using emulsification evaporation: using decompression Rotary Evaporators at 50 DEG C, 400r/min, pressure
For vacuum rotary steam under the conditions of -0.1MPa, ethyl alcohol and part water are removed；Then it is larger that 20min removing is centrifuged under 3000rpm revolving speed
Zeins-algae is made after dry to get zeins-propylene glycol alginate composite particles dispersion liquid is arrived in particle
Acid propylene glycol ester composite particles.
Concentration through composite particles in zeins obtained by the above method-propylene glycol alginate dispersion liquid is
1.0%, the three-phase contact angle of composite particles is 90 °, grain diameter 200-400nm；The three-phase contact angle figure of composite particles is such as
Shown in Fig. 2.
Pickering lotion is prepared by stabilizer of gained zeins-propylene glycol alginate composite particles: by institute
Obtaining the dispersion liquid that zeins-propylene glycol alginate composite particles are dispersed in water will walk as water phase, or directly
Suddenly zeins-propylene glycol alginate composite particles dispersion liquid obtained by (4) is water phase；It is oil with medium-chain fatty glyceride
Oil is mutually added in water phase by phase, and oil phase volume score is 50% (v/v), and Pickering is made in shearing 3min under 10000rpm
Lotion.The oil droplet size of gained Pickering lotion is 20~30 μm, and after 80 DEG C of heat treatment 30min, lotion is not divided
Layer and condensate oil phenomenon, oil droplet size maintain 20~30 μm；After placing 30 days, lotion does not occur lamination and condensate oil phenomenon, tool
There are preferable thermal stability and storage-stable；Gained Pickering lotion CLSM figure is as shown in Figure 3.
Embodiment 3
Alcohol soluble protein-polysaccharide composite particle is prepared as steps described below:
(1) 0.5g zeins is added in 50mL ethanol water (volume fraction of ethyl alcohol is 75%), often
Temperature stirring obtains zeins ethanol water to being completely dispersed；
(2) by 0.1g propylene glycol alginate, (zeins and propylene glycol alginate mass ratio add for 5:1) and exist
In 50mL ethanol water (volume fraction of ethyl alcohol is 75%), magnetic agitation obtains propylene glycol alginate second to being completely dissolved
Alcohol solution；
(3) zeins ethanol water obtained by step (1) is added to obtained by step (2) under stirring conditions
3.5h, which is stirred, in propylene glycol alginate ethanol water, after mixing obtains zeins-alginate propylene glycol ester admixture second
Alcohol solution；
(4) composite particles are prepared using emulsification evaporation: using decompression Rotary Evaporators at 55 DEG C, 600r/min, pressure
For vacuum rotary steam under the conditions of -0.1MPa, ethyl alcohol and part water are removed；Then it is larger that 25min removal is centrifuged under 3500rpm revolving speed
Zeins-algae is made after dry to get zeins-propylene glycol alginate composite particles dispersion liquid is arrived in particle
Acid propylene glycol ester composite particles.
Concentration through composite particles in zeins obtained by the above method-propylene glycol alginate dispersion liquid is
1.5%, 93 ° of the three-phase contact angle of particle, grain diameter 300-400nm.
Pickering lotion is prepared by stabilizer of gained zeins-propylene glycol alginate composite particles, by institute
Obtaining the dispersion liquid that zeins-propylene glycol alginate composite particles are dispersed in water will walk as water phase, or directly
Suddenly zeins-propylene glycol alginate composite particles dispersion liquid obtained by (4) is water phase；It is oily phase with edible corn oil, it will
Oil is mutually added in water phase, and oil phase volume score is 60% (v/v), and Pickering lotion is made in shearing 4min under 12000rpm.
The oil droplet size of gained Pickering lotion be 20~40 μm, after 85 DEG C of heat treatment 30min, lotion do not occur layering with
Condensate oil phenomenon, oil droplet size maintain 20~40 μm；After placing 30 days, lotion does not occur layering and condensate oil phenomenon, has preferable
Thermal stability and storage-stable.
Embodiment 4
Alcohol soluble protein-polysaccharide composite particle is prepared as steps described below:
(1) 0.6g zeins is added in 50mL ethanol water (volume fraction of ethyl alcohol is 85%), often
Temperature stirring obtains zeins ethanol water to being completely dispersed；
(2) 0.3g propylene glycol alginate (zeins and propylene glycol alginate mass ratio be 2:1) is added to
In 50mL ethanol water (volume fraction of ethyl alcohol is 85%), magnetic agitation obtains propylene glycol alginate second to being completely dissolved
Alcohol solution；
(3) under stirring conditions, zeins ethanol water obtained by step (1) is added to step (2) institute
It obtains in propylene glycol alginate ethanol water, it is water-soluble to obtain zeins-propylene glycol alginate ethyl alcohol by stirring 4h after mixing
Liquid；
(4) composite particles are prepared using emulsification evaporation: using decompression Rotary Evaporators at 60 DEG C, 800r/min, pressure
For vacuum rotary steam under the conditions of -0.1MPa, ethyl alcohol and part water are removed；Then it is larger that 25min removing is centrifuged under 3500rpm revolving speed
Zeins-algae is made after dry to get zeins-propylene glycol alginate composite particles dispersion liquid is arrived in particle
Acid propylene glycol ester composite particles.
The concentration of composite particles is 2.5% in gained zeins-propylene glycol alginate dispersion liquid, the three-phase of particle
92 ° of contact angle, grain diameter 500-600nm；
Pickering lotion is prepared by stabilizer of gained zeins-propylene glycol alginate composite particles: by institute
Obtaining the dispersion liquid that zeins-propylene glycol alginate composite particles are dispersed in water will walk as water phase, or directly
Suddenly zeins-propylene glycol alginate composite particles dispersion liquid obtained by (4) is water phase；It is oily phase with edible soybean oil, it will
Oil is mutually added in water phase, and oil phase volume score is 75% (v/v), and Pickering lotion is made in shearing 5min under 15000rpm.
The oil droplet size of phase Pickering lotion is 40~80 μm in gained height, and after 95 DEG C of heat treatment 30min, lotion does not occur
Layering and condensate oil phenomenon, oil droplet size maintain 40~80 μm；After placing 30 days, lotion does not occur layering and condensate oil phenomenon, has
Preferable thermal stability and storage-stable.
Claims (10)
1. a kind of alcohol soluble protein-polysaccharide composite particle preparation method, which is characterized in that zeins ethyl alcohol is water-soluble
Liquid, propylene glycol alginate ethanol water are mixed to get mixed solution in equal volume under stiring, and the mixed solution is revolved through decompression
It steams, centrifugation obtains alcohol soluble protein-polysaccharide composite particle dispersion, obtained alcohol soluble protein-polysaccharide composite particle after dry；
The alcohol soluble protein-polysaccharide composite particle prepares Pickering lotion as solid emulsifier.
2. preparation method according to claim 1, which is characterized in that the zeins ethanol water corn
Alcohol soluble protein and ethanol water are mixed to get, the propylene glycol alginate ethanol water propylene glycol alginate and ethanol water
Solution is mixed to get；Contain 0.1~50mg of zeins in every 1mL zeins ethanol water.
3. preparation method according to claim 2, which is characterized in that the volume fraction of ethyl alcohol is in the ethanol water
60%~90%.
4. preparation method according to claim 1, which is characterized in that zeins and alginic acid in the mixed solution
Propylene glycol ester mass ratio is 40:1~1:2；The mixing time of the mixed solution is 1~8h.
5. preparation method according to claim 1, which is characterized in that the temperature of the vacuum rotary steam is 35~65 DEG C, rotation
Rotary speed is 200~800r/min, and pressure is -0.1MPa；The centrifugal speed is 1500~6500rad/min, centrifugation time
For 10~60min.
6. preparation method according to claim 1, which is characterized in that the alcohol soluble protein-polysaccharide composite particle dispersion
Middle composite particles concentration is 0.25~10%.
7. method according to claim 1, which is characterized in that the mode of the drying is vacuum freeze drying, pre-freeze
1~12h is handled, pre-freezing temperature is -20~-50 DEG C, and drying time is 1~12h.
8. a kind of alcohol soluble protein-polysaccharide composite particle of any one of claim 1-7 preparation method preparation is in Pickering
Application in lotion, which is characterized in that mix the alcohol soluble protein-polysaccharide composite particle dispersion with oil, emulsification pretreatment
After obtain Pickering lotion.
9. application according to claim 8, which is characterized in that the oil is mutually edible oil and/or medium chain fatty acid
Ester.
10. application according to claim 8, which is characterized in that the oil mutually disperses with alcohol soluble protein-polysaccharide composite particle
The volume ratio of liquid mixing is 1:9~4:1；The shear velocity is 7000~18000rpm, and the time is 2~10min.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110547442A (en) * | 2019-09-20 | 2019-12-10 | 武汉轻工大学 | Whey protein isolate-tannic acid nanoparticle stabilizer, preparation method thereof and oil phase Pickering emulsion |
| CN110917137A (en) * | 2019-11-26 | 2020-03-27 | 江南大学 | Preparation method of ultrastable pickering emulsion with synergistic stability of prolamin nanoparticles and starch nanoparticles |
| CN111228420A (en) * | 2020-02-27 | 2020-06-05 | 佛山科学技术学院 | Preparation method of coix seed oil nano composite particles |
| CN111887424A (en) * | 2020-07-20 | 2020-11-06 | 武汉轻工大学 | High-stability Pickering emulsion and preparation method thereof |
| CN112120212A (en) * | 2020-09-23 | 2020-12-25 | 合肥工业大学 | Hyaluronic acid-based modified gliadin nanoparticle and preparation method and application thereof |
| CN112425660A (en) * | 2020-11-25 | 2021-03-02 | 安徽省含山县油脂有限公司 | Preparation method for improving stability of emulsified oil |
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| CN113455647A (en) * | 2021-07-08 | 2021-10-01 | 大连工业大学 | Preparation method and application of wheat alcohol-soluble protein/propylene glycol alginate Pickering emulsion |
| CN114957676A (en) * | 2022-06-22 | 2022-08-30 | 安徽工程大学 | Method for preparing Maillard reaction product by quickly regulating and controlling water activity through anti-solvent deposition |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110547442A (en) * | 2019-09-20 | 2019-12-10 | 武汉轻工大学 | Whey protein isolate-tannic acid nanoparticle stabilizer, preparation method thereof and oil phase Pickering emulsion |
| CN110917137A (en) * | 2019-11-26 | 2020-03-27 | 江南大学 | Preparation method of ultrastable pickering emulsion with synergistic stability of prolamin nanoparticles and starch nanoparticles |
| CN110917137B (en) * | 2019-11-26 | 2021-08-10 | 江南大学 | Preparation method of Pickering emulsion with synergistic and stable prolamin nanoparticles and starch nanoparticles |
| CN111228420A (en) * | 2020-02-27 | 2020-06-05 | 佛山科学技术学院 | Preparation method of coix seed oil nano composite particles |
| CN111887424A (en) * | 2020-07-20 | 2020-11-06 | 武汉轻工大学 | High-stability Pickering emulsion and preparation method thereof |
| CN111887424B (en) * | 2020-07-20 | 2023-02-03 | 武汉轻工大学 | High-stability Pickering emulsion and preparation method thereof |
| CN112120212B (en) * | 2020-09-23 | 2022-05-31 | 合肥工业大学 | Hyaluronic acid-based modified gliadin nanoparticle and preparation method and application thereof |
| CN112120212A (en) * | 2020-09-23 | 2020-12-25 | 合肥工业大学 | Hyaluronic acid-based modified gliadin nanoparticle and preparation method and application thereof |
| CN112425660A (en) * | 2020-11-25 | 2021-03-02 | 安徽省含山县油脂有限公司 | Preparation method for improving stability of emulsified oil |
| CN112704215A (en) * | 2020-12-28 | 2021-04-27 | 吉林农业大学 | Zein-gallic acid composite colloidal particle and preparation method of emulsion thereof |
| CN112957324A (en) * | 2021-02-08 | 2021-06-15 | 广东省科学院测试分析研究所(中国广州分析测试中心) | Preparation method and application of pickering emulsion by using prolamin to load eucommia chlorogenic acid |
| CN113455647A (en) * | 2021-07-08 | 2021-10-01 | 大连工业大学 | Preparation method and application of wheat alcohol-soluble protein/propylene glycol alginate Pickering emulsion |
| CN114957676A (en) * | 2022-06-22 | 2022-08-30 | 安徽工程大学 | Method for preparing Maillard reaction product by quickly regulating and controlling water activity through anti-solvent deposition |
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