CN112956483A - Compound globispirin A for inhibiting growth of weed seeds - Google Patents
Compound globispirin A for inhibiting growth of weed seeds Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
Abstract
The invention relates to a compound globispirin A (guignardone A) for inhibiting the growth of weed seeds, belonging to the field of herbicidal active substances. The invention researches the influence of the globefield A solution on the growth of two weed seeds of monocotyledon ryegrass and dicotyledon spanishneedles herb by adopting a culture dish biological monitoring method, and the result shows that the globefield A solution shows an inhibition effect on the growth of the ryegrass and the spanishneedles herb seeds, and has wide application prospect in the aspect of controlling the growth of weeds such as ryegrass, spanishneedles herb and the like.
Description
Technical Field
The invention belongs to the field of weeding active substances, relates to a compound globocin A for inhibiting the growth of ryegrass and bidens bipinnata seeds, and also relates to an application of the compound globocin A in preparing products for inhibiting the growth of the ryegrass and bidens bipinnata seeds.
Background
The occurrence of weeds in farmlands seriously threatens the yield of crops, and causes huge economic loss. Since the 70 s in the 20 th century, chemical herbicides have rapidly become the main treatment mode of agricultural field weeds due to the advantages of higher efficiency, labor saving, time saving and the like compared with artificial weeding modes. The single use of a large amount of chemical herbicides causes the change of field weed communities, the drug resistance of weeds develops rapidly, the amount of intractable populations is increased, and then the excessive use of the herbicides is serious, thereby causing vicious circle, endangering ecological balance and polluting the environment. Meanwhile, chemical herbicide residues have become an important factor affecting human health. With the deep promotion of the action of 'zero increase of pesticide usage' developed in China, how to scientifically and safely use pesticides and novel substitutes to achieve the purposes of reducing pesticides, improving quality and increasing efficiency is a goal of the research researchers. Based on the concern on the weed resistance problem and the ecological environment safety, the natural product herbicide is an important development direction of novel environment-friendly herbicides due to the advantages of various active ingredients, unique action mechanism, difficult occurrence of drug resistance of target pests, safety of non-target organisms such as human and livestock and the like. The development of natural herbicides can replace chemical herbicides, reduce the generation of weed resistance and reduce the residual quantity of the natural herbicides in crops or soil environments, and becomes a hot research field for solving the problem of agricultural sustainable development. Therefore, it is of great significance to search for safe and reliable effective substances for inhibiting the growth of weed seeds from natural products.
In recent years, weeds which are common in the fields of ryegrass and bidens are flooded in the fields to cause disasters, and the weeds compete for nutrient and moisture with crops to influence the growth of the crops. The inventor searches for a compound for inhibiting the growth of ryegrass and bidens bipinnata seed roots from natural products with small toxic and side effects and discovers the compound globocin A. Globefish A is a hybrid terpenoid secondary metabolite produced in the growth process of microorganisms of the genus Globius, was originally isolated from Iregypus mangiferus in 2010, and the total synthesis of the compound was achieved in 2020 (Eur. J. org. chem., 2010, 33, 6348-19-6353; org. Lett., 2020, 22, 1644-1647). The research shows that the globocin A has the activities of resisting bacteria, tumors and the like. Through the search of documents in the prior art, no document report that the coccidiostatin A inhibits the growth of ryegrass and sticktight seeds exists, and no herbicide preparation related to the coccidiostatin A exists in the market.
Disclosure of Invention
The inventor searches for a compound for inhibiting the growth of ryegrass and bidens bipinnata seeds from natural products with small toxic and side effects, and finds that the compound of the globispin A has a remarkable inhibiting effect on the growth of the ryegrass and bidens bipinnata seeds, thereby completing the invention.
The invention aims to overcome the defects of the prior art and provide a compound of the globispirin A for inhibiting the growth of ryegrass and bidens bipinnata seeds, and the invention also aims to provide the application of the compound of the globispin A in preparing products for inhibiting the growth of the ryegrass and bidens bipinnata seeds.
In order to achieve the above purpose, the invention adopts the technical scheme that:
the compound of the invention is the globocin A for inhibiting the growth of weed seeds, the globocin A is a compound with the following structural formula,。
the compound globisphin A disclosed by the invention is applied to preparation of products for inhibiting growth of ryegrass seeds.
The compound of the invention, the globispirin A, is applied to the preparation of products for inhibiting the growth of bidens bipinnata seeds.
Further, the application of the compound globisphin A in preparing products for inhibiting growth of ryegrass seeds is that the solution concentration of the compound globisphin A is 100-1000-μM。
Further, the application of the compound globisphin A in preparing products for inhibiting the growth of bidens bipinnata seeds is that the solution concentration of the compound globisphin A is 100-1000-μM。
Preferably, the compound of the invention, the concentration of the compound of the globisphin A in the solution is 1000μM。
Preferably, the compound of the invention, the concentration of the solution of the compound of the globisphin A is 1000μM。
The invention adopts the culture dish biological monitoring method to researchThe influence of the globocin A solution on the growth of monocotyledonous ryegrass and dicotyledonous plant sticktight is examined by taking the length of the seedling embryo root as an index. The result shows that the globinclysin A solution has an inhibition effect on the growth of radicle of ryegrass seeds, the inhibition effect is enhanced along with the increase of the concentration of the globinclysin A solution, and the concentration of the globinclysin A solution is 10μM、33 μM、100μM、333 μM and 1000μThe inhibition rate of M on the radicle growth of the ryegrass seed is 11.63%, 16.90%, 24.92%, 46.83% and 70.46%, respectively. The coccidiostatin A solution has inhibitory effect on growth of embryo root of Bidens bipinnata seed, and the inhibitory effect is increased with increase of the concentration of the coccidiostatin A solution, wherein the concentration of the coccidiostatin A solution is 10μM、33 μM、100μM、333 μM and 1000μThe inhibition rate of M on the growth of the radicle of the bidens bipinnata seed is 11.64 percent, 12.86 percent, 21.81 percent, 32.53 percent and 72.10 percent respectively. The results show that the compound globefield A is applied to the preparation of products for inhibiting the growth of ryegrass and sticktight, in particular the solution concentration of the compound globefield A is 1000μThe application of M in preparing products for inhibiting the growth of ryegrass and sticktight. The compound of the globispirin A can also be applied to the preparation of products for inhibiting the growth of ryegrass and sticktight.
The compound globisphin A for inhibiting the growth of the ryegrass seeds has the advantages of no toxicity to people and livestock, no harm to ecological balance and no environmental pollution.
The compound of the geldanamycin A for inhibiting the growth of the bidens bipinnata seeds has the advantages of no toxicity to people and livestock, no harm to ecological balance and no environmental pollution.
The invention also provides a product prepared by using the compound of the invention for inhibiting the growth of ryegrass and bidens bipinnata seeds and the acceptable carrier or excipient. These products are selected from the following dosage forms: wettable powder, granules, water aqua, soluble powder and tablets.
The product containing the compound of the invention for inhibiting the growth of the ryegrass and the bidens bipinnata seeds can be added with an acceptable carrier when the product is prepared, and the acceptable carrier of the product is from the following components: antioxidant, chelating agent, surfactant, filler, disintegrant, wetting agent, solvent, pH regulator, etc., and commonly used carriers such as: mannitol, dextran, lactose, glucose, sorbitol, xylitol, water, silicon derivatives, cellulose derivatives, gelatin, polyvinylpyrrolidone, glycerol, tween 80, agar, calcium carbonate, polyethylene glycol, cyclodextrin, phospholipid material, talc, magnesium stearate, calcium stearate, etc.
The present invention is further described below with reference to specific examples, but the present invention is not limited thereto, and embodiments of the present invention are not limited to the specific examples according to the prior art known in the art.
Detailed Description
Example 1
Effect of the Compound globocin A on seed growth of ryegrass
Preparation of different concentrations of globocin A solution A is prepared by weighing appropriate amount of globocin A, dissolving with distilled water, and preparing into 0μM (distilled water, blank control), 1μM、3 μM、10μM、33 μM、100 μM、333 μM、1000 μM in globocin A.
Selecting ryegrass seeds with full seeds, uniform size and no plant diseases and insect pests, sterilizing with 10% sodium hypochlorite for 10 minutes, washing with distilled water for 5 times, placing on filter paper, and volatilizing in a sterile operating platform for later use.
Ryegrass seed growth assay
The petri dish filter paper method is adopted. Double-layer filter paper is filled on the bottom of the culture dish, and after high-temperature sterilization, the double-layer filter paper is respectively added with the concentration of 1μM、3 μM、10μM、33 μM、100 μM、333 μM、1000 μM aqueous solution of coccidiostatin A (6 ml, 0)μSetting 30 germinated ryegrass seeds in each culture dish as a control group for M concentration group and experimental groups for the rest concentration groups, setting three parallel groups for different concentration groups, and culturing the culture dishes in an intelligent artificial incubator (illumination intensity of 3000 lx, illumination time of 12 h, temperature of 25 +/-1 ℃ and humidity of 80% RH) for 5 daysAnd measuring the length of the embryonic root and the length of the embryonic axis and calculating the inhibition rate. The results are shown in Table 1.
TABLE 1 Effect of the Compound globispin A on seed growth of ryegrass
P <0.5 compared to control, P <0.05 compared to control.
Example 2
Effect of the Compound globispirin A on seed growth of Bidens bipinnata
Preparation of different concentrations of globocin A solution A is prepared by weighing appropriate amount of globocin A, dissolving with distilled water, and preparing into 0μM (distilled water, blank control), 1μM、3 μM、10μM、33 μM、100 μM、333 μM、1000 μM in globocin A.
Selecting the bidens bipinnata seeds with plump seeds, uniform size and no plant diseases and insect pests, sterilizing with 10% sodium hypochlorite for 10 min, washing with distilled water for 5 times, placing on filter paper, and volatilizing in a sterile operating platform for later use.
Bidens pilosa seed growth assay
The petri dish filter paper method is adopted. Double-layer filter paper is filled on the bottom of the culture dish, and after high-temperature sterilization, the double-layer filter paper is respectively added with the concentration of 1μM、3 μM、10μM、33 μM、100 μM、333 μM、1000 μM aqueous solution of coccidiostatin A (6 ml, 0)μThe M concentration group is a control group, the other concentration groups are experimental groups, 30 germinated barnyard grass seeds are placed in each culture dish respectively, three groups with different concentrations are arranged in parallel respectively, the culture dishes are placed in an intelligent artificial incubator (the illumination intensity is 3000 lx, the illumination time is 12 h, the temperature is 25 +/-1 ℃, the humidity is 80% RH) to be cultured for 4 days, the length of the radicle and the length of the axis of the embryo are measured, and the inhibition rate is calculated. The results are shown in Table 2.
TABLE 2 Effect of the Compound globisparin A on the growth of Bidens pilosa seeds
P <0.5 compared to control, P <0.05 compared to control.
Example 3
A product prepared from the compound of the globulina for inhibiting the growth of the ryegrass seeds and an acceptable carrier or excipient. These products are selected from the following dosage forms: wettable powder, granules, water aqua, soluble powder and tablets. An acceptable carrier may be added in the preparation of the product, said product acceptable carrier being derived from: antioxidant, chelating agent, surfactant, filler, disintegrant, wetting agent, solvent, pH regulator, etc., and commonly used carriers such as: mannitol, dextran, lactose, glucose, sorbitol, xylitol, water, silicon derivatives, cellulose derivatives, gelatin, polyvinylpyrrolidone, glycerol, tween 80, agar, calcium carbonate, polyethylene glycol, cyclodextrin, phospholipid material, talc, magnesium stearate, calcium stearate, etc. The preparation processes are all conventional operations in the field, and are not described herein.
Example 4
A product prepared from the compound of the gelonin A for inhibiting the growth of the seeds of the bidens bipinnata and an acceptable carrier or excipient. These products are selected from the following dosage forms: wettable powder, granules, water aqua, soluble powder and tablets. An acceptable carrier may be added in the preparation of the product, said product acceptable carrier being derived from: antioxidant, chelating agent, surfactant, filler, disintegrant, wetting agent, solvent, pH regulator, etc., and commonly used carriers such as: mannitol, dextran, lactose, glucose, sorbitol, xylitol, water, silicon derivatives, cellulose derivatives, gelatin, polyvinylpyrrolidone, glycerol, tween 80, agar, calcium carbonate, polyethylene glycol, cyclodextrin, phospholipid material, talc, magnesium stearate, calcium stearate, etc. The preparation processes are all conventional operations in the field, and are not described herein.
Example 5
The product prepared by taking the compound of the globinclusin A for inhibiting the growth of the ryegrass seeds as a raw material not only comprises the product of the compound of the globinclusin A for inhibiting the growth of the ryegrass seeds which is used independently, but also comprises the product of the compound of the globinclusin A for inhibiting the growth of the ryegrass seeds which is added as an active ingredient.
Example 6
The product prepared by taking the compound of the globisprin A for inhibiting the growth of the bidens bipinnata seeds as a raw material not only comprises the product of the compound of the globisprin A for inhibiting the growth of the secale grass seeds by single use, but also comprises the product of the compound of the globisprin A for inhibiting the growth of the secale grass seeds as an active ingredient.
Claims (9)
2. use of the compound globisphin A according to claim 1 for the preparation of a product for inhibiting the growth of rye grass seeds.
3. Use of the compound globisphin A according to claim 1 for the preparation of a product for inhibiting the growth of Bidens bipinnata seeds.
4. The use as claimed in claim 1 or 2, wherein the compound globinclusin A is used in the preparation of a product for inhibiting the growth of rye grass seeds, and the solution concentration of the compound globinclusin A is 100-1000 μ M.
5. Use according to claim 1 or 2, wherein the compound globinclycin A is used in the preparation of a product for inhibiting the growth of rye grass seeds, and the concentration of the compound globinclycin A in a solution is 1000 μ M.
6. The use as claimed in claim 1 or 3, wherein the compound globinclusin A is used in the preparation of a product for inhibiting the growth of Bidens pilosa seeds, and the concentration of the solution of the compound globinclusin A is 100 μ M and 1000 μ M.
7. Use according to claim 1 or 3, wherein the compound globisphin A is used in the preparation of a product for inhibiting the growth of Bidens bipinnata seeds, and the concentration of the solution of the compound globisphin A is 1000. mu.M.
8. Use of a composition comprising the compound globisphin a of claim 1 in the preparation of a product for inhibiting the growth of rye grass seeds.
9. Use of a composition comprising the compound geotrichin a of claim 1 for the preparation of a product for inhibiting the growth of seeds of Bidens bipinnata.
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CN115011487A (en) * | 2022-05-12 | 2022-09-06 | 宁波大学 | Sponge epiphytic fungus and application thereof in preparation of terpenoid compounds |
CN115011487B (en) * | 2022-05-12 | 2023-08-18 | 宁波大学 | Sponge coaptation fungus and application thereof in preparation of hetero-terpenoid |
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