CN112941095A - Recombinant vector of panda rotavirus CH-1 strain VP7 protein, genetic engineering bacteria and application thereof - Google Patents

Recombinant vector of panda rotavirus CH-1 strain VP7 protein, genetic engineering bacteria and application thereof Download PDF

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CN112941095A
CN112941095A CN202110278115.7A CN202110278115A CN112941095A CN 112941095 A CN112941095 A CN 112941095A CN 202110278115 A CN202110278115 A CN 202110278115A CN 112941095 A CN112941095 A CN 112941095A
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protein
rotavirus
strain
panda
recombinant vector
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陈翠翠
梁焕坤
昝洁
赖宏锐
何莹
郭桂铃
李来庆
宁波
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Guangzhou Youdi Biotechnology Co ltd
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    • C12N2720/12311—Rotavirus, e.g. rotavirus A
    • C12N2720/12322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
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    • G01N2333/14—Reoviridae, e.g. rotavirus, bluetongue virus, Colorado tick fever virus

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Abstract

The invention relates to the field of genetic engineering, and particularly discloses a recombinant vector of panda rotavirus CH-1 strain VP7 protein, a genetically engineered bacterium and application thereof. The recombinant vector contains the coding gene of the protein VP7 of the panda rotavirus CH-1 strain. The protein VP7 of the panda rotavirus CH-1 strain expressed by the engineering bacteria containing the recombinant vector has good biological activity and immunogenicity, the protein expression amount is large, the production cost is greatly reduced, and a foundation is laid for the development of a detection kit of a panda rotavirus (GPRV) CH-1 strain.

Description

Recombinant vector of panda rotavirus CH-1 strain VP7 protein, genetic engineering bacteria and application thereof
Technical Field
The invention relates to the field of genetic engineering, in particular to a recombinant vector of panda rotavirus CH-1 strain VP7 protein, a genetically engineered bacterium and application thereof.
Background
Panda Rotavirus (GPRV) CH-1 strain was first detected and isolated from panda diarrhea feces in the year 2009 from wangdong, et al. The strain can cause infectious and refractory diarrhea of young pandas (5-11 months old), so that multiple organ failure and death are caused, and a certain influence is generated on the expansion of panda populations. At present, no effective vaccine or specific medicine is available for preventing and treating the GPRV infection, which poses serious threat to the health of pandas.
Rotavirus belongs to the reoviridae, Rotavirus genus, and its genome contains 11 segments of dsRNA. The virus is in a wheel shape without a capsule membrane, and consists of three layers of protein capsids, namely VP7, VP4 and VP6 proteins. VP7 is a glycoprotein encoded by gene 9 (or 7, 8) and is an important protective antigen of rotavirus. The VP7 protein can independently induce the body to produce neutralizing antibody, which determines the G serotype of the virus. However, at present, no in vitro rapid detection kit for the CH-1 strain of Giant Panda Rotavirus (GPRV) exists, so that the establishment of an in vitro expression system of the VP7 protein of the CH-1 strain of the Giant Panda Rotavirus (GPRV) is very important for the development of a rapid detection method for the CH-1 strain of the Giant Panda Rotavirus (GPRV).
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a recombinant vector of the protein VP7 of the CH-1 strain of panda rotavirus, a genetically engineered bacterium and application thereof, wherein the protein VP7 of the CH-1 strain of panda rotavirus, which is expressed by the genetically engineered bacterium containing the recombinant vector, has good biological activity and immunogenicity.
In the first aspect of the invention, the invention provides a recombinant vector, which contains a coding gene of a giant panda rotavirus CH-1 strain VP7 protein.
As a preferred embodiment of the recombinant vector, the recombinant vector is obtained by inserting the coding gene of the protein VP7 of the CH-1 strain of the panda rotavirus into an original vector. The starting vector may be a vector conventional in the art, such as a commercially available plasmid, phage or viral vector.
As a preferred embodiment of the recombinant vector of the present invention, the starting vector is a plasmid. The plasmid is preferably pCold II.
The second aspect of the invention provides a genetic engineering bacterium for expressing the protein VP7 of the panda rotavirus CH-1 strain, wherein the genetic engineering bacterium contains the coding gene of the protein VP7 of the panda rotavirus CH-1 strain.
As a preferred embodiment of the gene engineering bacteria, the gene engineering bacteria are obtained by introducing a recombinant vector containing a giant panda rotavirus CH-1 strain VP7 protein coding gene into a host bacterium.
As a preferred embodiment of the genetically engineered bacterium of the present invention, the host bacterium is Escherichia coli. The host bacterium is preferably competent for Escherichia coli pTf16/BL21(DE 3).
As a preferred embodiment of the gene engineering bacteria, the coding gene of the protein VP7 of the panda rotavirus CH-1 strain is a gene with a nucleotide sequence shown as SEQ ID NO. 1.
As a preferred embodiment of the gene engineering bacteria, the protein of the giant panda rotavirus CH-1 strain VP7 is a protein with an amino acid sequence shown as SEQ ID NO. 2, or a protein with the activity of the giant panda rotavirus CH-1 strain VP7 protein after the SEQ ID NO. 2 is substituted, deleted or added by one or more amino acid residues.
The third aspect of the invention provides a preparation method of a genetic engineering bacterium for expressing a panda rotavirus CH-1 strain VP7 protein, which is characterized by comprising the following steps: the above recombinant vector is introduced into a host bacterium.
The fourth aspect of the invention provides a preparation method of giant panda rotavirus CH-1 strain VP7 protein, which is characterized in that the preparation method comprises the steps of utilizing the genetic engineering bacteria to induce expression, collecting and crushing thallus, collecting inclusion bodies, and then dissolving and renaturing the obtained inclusion bodies to obtain the giant panda rotavirus CH-1 strain VP7 protein.
The fifth aspect of the invention provides an application of giant panda rotavirus CH-1 strain VP7 protein in preparing a detection kit.
Compared with the prior art, the invention has the following beneficial effects:
1) the invention adopts an escherichia coli expression system to express the protein VP7 of the CH-1 strain of panda rotavirus (GPRV), has large protein expression amount and simple purification method, greatly reduces the production cost and is beneficial to promoting mass production;
2) the invention utilizes an escherichia coli prokaryotic expression system to express the protein VP7 of the CH-1 strain of Giant Panda Rotavirus (GPRV) in the form of an inclusion body, the protein has better biological activity and immunogenicity, the production cost is greatly reduced, and a foundation is laid for the development of a detection kit of the CH-1 strain of the Giant Panda Rotavirus (GPRV).
Drawings
FIG. 1 is a SDS-PAGE gel staining diagram of protein purification of Giant Panda Rotavirus (GPRV) CH-1 strain VP 7.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to the accompanying drawings and specific embodiments.
The experimental procedures used in the following examples are conventional unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The nucleotide sequence of the coding gene of the protein VP7 of the panda rotavirus CH-1 strain in the embodiment of the invention is shown in SEQ ID NO. 1.
The amino acid sequence of the protein VP7 of the panda rotavirus CH-1 strain is shown as SEQ ID NO. 2.
Example 1 construction of genetically engineered bacterium expression vector for expressing protein VP7 of panda rotavirus CH-1 strain
1. Synthesis of the Gene of interest
The Guangzhou Asia biotechnology limited company is entrusted to synthesize the coding gene sequence (shown as SEQ ID NO: 1) of the protein VP7 of the CH-1 strain of panda rotavirus, and the XhoI enzyme digestion sequence (CTCGAG) is added at the 5 'end and the EcoRI enzyme digestion sequence (GAATTC) is added at the 3' end of the coding gene sequence of the protein VP7 of the CH-1 strain of panda rotavirus. Then, the pCold II plasmid and EcoRI enzyme were digested with XhoI enzyme (TaKaRa) and EcoRI enzyme (TaKaRa), respectively, and the double digestion products were recovered with PCR product purification recovery kit, respectively. After recovery, ligation was performed overnight at 16 ℃ with T4 DNA ligase, and the ligation product was transformed into competent E.coli DH5 a. After the extracted plasmid is sequenced and identified correctly, a recombinant vector for expressing the protein VP7 of the CH-1 strain of panda rotavirus (GPRV) is obtained, and the recombinant plasmid is named as pCold II-GPRV-CH-1-VP 7.
2. Screening of genetically engineered bacteria
Transforming the plasmid pCold II-GPRV-CH-1-VP 7 obtained in the step (1) into escherichia coli competence pTf16/BL21(DE 3); obtaining a genetic engineering bacterium for expressing a Giant Panda Rotavirus (GPRV) CH-1 strain VP7 protein, and naming the genetic engineering bacterium as E.coli-GPRV-CH-1-VP 7;
3. inducing expression by inclusion bodies: culturing the genetic engineering bacteria E.coli-GPRV-CH-1-VP7 obtained in the step 2, and inducing expression protein by using IPTG;
4. and (3) inclusion body purification: centrifugally collecting the genetic engineering bacteria obtained in the step 3, ultrasonically crushing the bacteria, centrifugally removing supernatant, scraping bacterial sludge on the upper layer of sediment, and collecting inclusion bodies;
5. denaturation and renaturation of inclusion bodies: dissolving the inclusion body by using an inclusion body dissolving solution, slowly adding the dissolved inclusion body into an inclusion body renaturation solution for dilution renaturation, and stirring for 48 hours at 4 ℃; the formula of the renaturation liquid is as follows: 50mM Tris-HCl, 400mM L-arginine, pH adjusted to 8.0;
6. concentration and purification: slowly adding the replacement solution into the inclusion body renaturation solution, and concentrating at 4 ℃ to finally obtain concentrated panda rotavirus (GPRV) CH-1 strain VP7 protein; wherein, the formula of the replacement liquid is as follows: 20mM Tris-HCl, 50mM NaCl, pH adjusted to 8.0.
Wherein, the SDS-PAGE gel staining pattern of the protein of the CH-1 strain VP7 of Giant Panda Rotavirus (GPRV) is shown in figure 1.
Example 2 animal experiments
Mixing 80 mu g of Giant Panda Rotavirus (GPRV) CH-1 strain VP7 protein with an equal amount of Freund's complete adjuvant, completely emulsifying, injecting Balb/C mice by a back subcutaneous four-point injection method, mixing 100 mu g of Giant Panda Rotavirus (GPRV) CH-1 strain VP7 protein with Freund's incomplete adjuvant for a second immunization after three weeks, immunizing once every two weeks, and obtaining a serum titer of 1: 640000 above.
Mixing 600 mu g of panda rotavirus (giant panda rotavirus, GPRV) CH-1 strain VP7 protein and an equal amount of Freund's complete adjuvant, completely emulsifying, injecting a albino rabbit by using four points below the back and two points of popliteal lymph nodes, after three weeks, mixing 800 mu g of panda rotavirus (giant panda rotavirus, GPRV) CH-1 strain VP7 protein and Freund's incomplete adjuvant, carrying out second immunization by the same method, and then immunizing once every two weeks, wherein the total immunization is 5 times, and the 5-immune serum titer reaches 1: 1280000 above.
Animal experiments prove that high-level antibodies can be generated by immunizing mice and rabbits with the CH-1 strain VP7 protein of the panda rotavirus (GPRV) prepared by the invention, and a foundation is laid for establishing a detection method.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
SEQUENCE LISTING
<110> Guangzhou Youdi Biotechnology Ltd
<120> recombinant vector of giant panda rotavirus CH-1 strain VP7 protein, genetically engineered bacterium and application thereof
<130> 2020.12.29
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 1042
<212> DNA
<213> coding gene of giant panda rotavirus CH-1 strain VP7 protein
<400> 1
ccgtctggct agcggttagc tccttttaat gtatggtatt gaatatacca caattctaat 60
ctttctgata tcaatcattc tattcaatta tatattaaag tcagtaactc gaacgatgga 120
ctatattatt tacagatttc tattaataac ggcagcacta cttgcattta caaaagcaca 180
gaattacgga attaatctac ctataacagg atcaatggat actgcatacg ctaattcaac 240
tcaagaagaa acattcttga catctacatt atgcttatat tatccgactg aagcgagtaa 300
tcaaataaac gatggtgaat ggaaagacac gttatctcaa atgtttctta caaaaggatg 360
gccgacagga tcagtttatt ttaaagaata ctcaagtatc gtagacttct cagtcgatcc 420
acaattatac tgtgattaca atttggtact aatgaaatat gatcaaaatc ttgaattaga 480
tatgtcagag ttagctgatt taatactgaa tgaatggcta tgtaatccaa tggacataac 540
tttatattat tatcaacaga caggagaatc aaataaatgg atatcaatgg gatcatcatg 600
tactattaaa gtttgtccac tgaatacaca gacactagga ataggctgtc aaacgacgca 660
tgtagactca tttgagatcg ttgctgaaaa tgaaaaacta gctatagtgg atgtcgttga 720
tggcatagat cataaaataa atttaacaac tactacatgc acaattcgaa attgtaagaa 780
gctgggacct agagaaaacg tagctgtaat acaggttgga ggctctgata tattagatat 840
tacagcagat ccaacgacca atccacagac tgagagaatg atgagagtga attggaaaaa 900
gtggtggcaa gttttttata caatagttga ttatattaat caaattgtgc aagtaatgtc 960
caaaagatca cgatcgctaa acgcagcagc cttctattat agagtataga tatatcttag 1020
attagaattg tatgatgtga cc 1042
<210> 2
<211> 326
<212> PRT
<213> panda rotavirus CH-1 strain VP7 protein
<400> 2
Met Tyr Gly Ile Glu Tyr Thr Thr Ile Leu Ile Phe Leu Ile Ser Ile
1 5 10 15
Ile Leu Phe Asn Tyr Ile Leu Lys Ser Val Thr Arg Thr Met Asp Tyr
20 25 30
Ile Ile Tyr Arg Phe Leu Leu Ile Thr Ala Ala Leu Leu Ala Phe Thr
35 40 45
Lys Ala Gln Asn Tyr Gly Ile Asn Leu Pro Ile Thr Gly Ser Met Asp
50 55 60
Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu Thr Phe Leu Thr Ser Thr
65 70 75 80
Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ser Asn Gln Ile Asn Asp Gly
85 90 95
Glu Trp Lys Asp Thr Leu Ser Gln Met Phe Leu Thr Lys Gly Trp Pro
100 105 110
Thr Gly Ser Val Tyr Phe Lys Glu Tyr Ser Ser Ile Val Asp Phe Ser
115 120 125
Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn Leu Val Leu Met Lys Tyr
130 135 140
Asp Gln Asn Leu Glu Leu Asp Met Ser Glu Leu Ala Asp Leu Ile Leu
145 150 155 160
Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln
165 170 175
Gln Thr Gly Glu Ser Asn Lys Trp Ile Ser Met Gly Ser Ser Cys Thr
180 185 190
Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys Gln
195 200 205
Thr Thr His Val Asp Ser Phe Glu Ile Val Ala Glu Asn Glu Lys Leu
210 215 220
Ala Ile Val Asp Val Val Asp Gly Ile Asp His Lys Ile Asn Leu Thr
225 230 235 240
Thr Thr Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu
245 250 255
Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp Ile Leu Asp Ile Thr
260 265 270
Ala Asp Pro Thr Thr Asn Pro Gln Thr Glu Arg Met Met Arg Val Asn
275 280 285
Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Ile Val Asp Tyr Ile Asn
290 295 300
Gln Ile Val Gln Val Met Ser Lys Arg Ser Arg Ser Leu Asn Ala Ala
305 310 315 320
Ala Phe Tyr Tyr Arg Val
325

Claims (10)

1. A recombinant vector is characterized by containing a coding gene of a giant panda rotavirus CH-1 strain VP7 protein.
2. The recombinant vector according to claim 1, wherein the recombinant vector is obtained by inserting a coding gene of the protein VP7 of the CH-1 strain of panda rotavirus into an original vector.
3. The recombinant vector of claim 2, wherein the starting vector is a plasmid.
4. The recombinant vector of claim 3, wherein the plasmid is pCold II.
5. A gene engineering bacterium for expressing the protein VP7 of the CH-1 strain of panda rotavirus is characterized by containing the coding gene of the protein VP7 of the CH-1 strain of panda rotavirus.
6. The genetically engineered bacterium of claim 5, wherein the genetically engineered bacterium is obtained by introducing a recombinant vector containing a panda rotavirus CH-1 strain VP7 protein encoding gene into a host bacterium.
7. The genetically engineered bacterium of claim 5, wherein the coding gene of the protein VP7 of the CH-1 strain of panda rotavirus is a gene with a nucleotide sequence shown as SEQ ID NO. 1.
8. The genetically engineered bacterium of claim 5, wherein the protein of the panda rotavirus CH-1 strain VP7 is a protein with an amino acid sequence shown as SEQ ID NO. 2, or a protein with the panda rotavirus CH-1 strain VP7 protein activity after the amino acid residue of the SEQ ID NO. 2 is substituted, deleted or added.
9. A preparation method of a genetic engineering bacterium for expressing a panda rotavirus CH-1 strain VP7 protein is characterized by comprising the following steps: introducing the recombinant vector of any one of claims 1-4 into a host bacterium.
10. A preparation method of giant panda rotavirus CH-1 strain VP7 protein is characterized in that the giant panda rotavirus CH-1 strain VP7 protein is obtained by utilizing the genetic engineering bacteria of any one of claims 5 to 8 to induce expression, collecting and crushing the bacteria, collecting inclusion bodies, and then dissolving and renaturing the obtained inclusion bodies.
CN202110278115.7A 2021-03-15 2021-03-15 Recombinant vector of panda rotavirus CH-1 strain VP7 protein, genetic engineering bacteria and application thereof Pending CN112941095A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113433315A (en) * 2021-06-24 2021-09-24 广州优迪生物科技股份有限公司 Reagent strip for detecting panda rotavirus CH-1 strain antigen and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111635910A (en) * 2020-06-09 2020-09-08 成都大熊猫繁育研究基地 Panda rotavirus CH-1 strain VP7 positive plasmid and construction method and application thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111635910A (en) * 2020-06-09 2020-09-08 成都大熊猫繁育研究基地 Panda rotavirus CH-1 strain VP7 positive plasmid and construction method and application thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GUO,L. ET AL.: "ACCESSION NO:GU188284,Giant panda rotavirus A strain CH-1 glycosylated outer capsid protein gene,complete cds", 《GENBANK》 *
文继锋 等: "三种融合标签对大熊猫轮状病毒结构蛋白VP6 -VP7可溶性表达的影响", 《浙江农业学报》 *
王杭军 等: "草鱼呼肠孤病毒GCRV-GD108株VP5蛋白的原核表达", 《华中农业大学学报》 *
贡成良 等: "《生物化学与分子生物学实验指导》", 30 June 2010 *
颜其贵 等: "大熊猫轮状病毒CH-1株外衣壳蛋白（VP7）基因的克隆和生物信息学分析", 《畜牧兽医学报》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113433315A (en) * 2021-06-24 2021-09-24 广州优迪生物科技股份有限公司 Reagent strip for detecting panda rotavirus CH-1 strain antigen and preparation method thereof

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