CN113527516A - A-type seneca virus genetic engineering composite epitope protein, vaccine and application thereof - Google Patents

A-type seneca virus genetic engineering composite epitope protein, vaccine and application thereof Download PDF

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CN113527516A
CN113527516A CN202110801177.1A CN202110801177A CN113527516A CN 113527516 A CN113527516 A CN 113527516A CN 202110801177 A CN202110801177 A CN 202110801177A CN 113527516 A CN113527516 A CN 113527516A
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张中旺
潘丽
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Lanzhou Veterinary Research Institute of CAAS
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Abstract

The invention provides a type-A seneca virus genetic engineering composite epitope protein, a vaccine and application thereof, belonging to the technical field of veterinary biological products. The invention provides an amino acid sequence of a type A seneca virus genetic engineering composite epitope protein as shown in SEQ ID NO: as shown at 14. After the recombinant composite epitope protein is obtained through in vitro recombinant expression, the vaccine prepared by mixing the recombinant composite epitope protein with an adjuvant can detect the generation of specific antibodies and neutralizing antibodies when immunizing healthy and susceptible piglets for 28 days, and the composite epitope protein rP2 protected by the application has the virus attack protection rate of more than 80 percent and has higher virus attack protection effect compared with a control composite epitope protein. The prepared novel A-type seneca virus genetic engineering composite epitope protein vaccine has wide application prospect in the aspect of preventing and controlling A-type seneca virus.

Description

A-type seneca virus genetic engineering composite epitope protein, vaccine and application thereof
Technical Field
The invention belongs to the technical field of veterinary biological products, and particularly relates to a type A seneca virus genetic engineering composite epitope protein, a vaccine and application thereof.
Background
The epicaivirus disease is a viral infectious disease mainly infecting pigs caused by a type a epicai virus a (SVA). The clinical symptoms are very similar to foot-and-mouth disease, swine vesicular disease and vesicular stomatitis, and are characterized in that blister, ulceration and even hoof shell shedding occur at the parts of a pig nose and mouth mucosa, hoof crowns and the like, diarrhea symptoms are occasionally seen, the disease condition is rapid, and the death rate of newborn piglets (within 4 days of age) is up to 30-70%. The disease has great potential threat to the production and economic benefit of the pig industry, so the prevention and control of the disease should be emphasized. In 2018, 6 and 20 months, the agricultural rural department issues a notice about the completion of work on preventing and treating the Seneca virus disease, and the concern of the industry on the Seneca virus disease is caused.
Senecavirus type a belongs to the picornaviridae family in classification, the genus senecavirus, of which senecavirus type a is the only member. SVA has typical structural features of small RNA viruses. There are many irregular circular structures on the structural proteins VP1 and VP2 that are associated with viral adsorption, invasion, protective immune response, and serotype specificity. Research shows that SVA can induce body to generate B cell and T cell immune response, and VP1 protein contains multiple neutralizing structural domains and is the most important immunogenic protein.
Vaccination is a reliable and effective means for specifically preventing and controlling various epidemic diseases, but at present, only a few studies evaluate the immune response of a host to SVA infection, the study on SVA vaccine is in an exploration stage, only individual researchers such as yang and the like carry out preliminary study on SVA inactivated vaccine, no commercial vaccine is available, and no specific treatment method is available. The epitope vaccine is a novel vaccine which is safer and more reliable and is convenient for large-scale production, and has wide and successful application in the prevention and treatment process of epidemic diseases such as foot-and-mouth disease, malaria, hepatitis and the like. At present, the research on the SVA epitope vaccine is not reported in documents, and the research and development of the safe and efficient SVA epitope vaccine have important scientific and application values.
Disclosure of Invention
In view of the above, the invention aims to provide a composite epitope protein of a type-A seneca virus genetic engineering, a vaccine and an application thereof, wherein the composite epitope protein can generate a good immune protection effect, and provides a basis for preparing a safe and efficient novel genetic engineering vaccine of the seneca virus.
The invention provides a gene engineering composite epitope protein of an A-type seneca virus, which comprises a B cell epitope and a T cell epitope;
the B cell epitope comprises VP1 protein, VP2 protein and VP3 protein of the type A seneca virus; the VP1 protein comprises an amino acid sequence shown as SEQ ID NO:1 to SEQ ID NO: 4, fragment 1 to fragment 4;
the VP2 protein comprises an amino acid sequence shown as SEQ ID NO:5 to SEQ ID NO: 9, segment 5 to segment 9;
the VP3 protein comprises an amino acid sequence shown as SEQ ID NO: 10, segment 10;
the T cell epitope comprises PADRE and invasin;
the amino acid sequence of the PADRE is shown as SEQ ID NO:11 is shown in the figure;
the amino acid sequence of the invasin is shown as SEQ ID NO: shown at 12.
Preferably, the composite epitope protein is PADRE-fragment 5-fragment 6-fragment 7-fragment 8-fragment 9-fragment 10-fragment 1-fragment 2-fragment 3-fragment 4-invasin.
Preferably, the complex epitope protein further comprises a connecting peptide;
the B cell epitopes are connected by a connecting peptide GGSSGG; the B cell epitope and the T cell epitope are connected by a connecting peptide GGC.
Preferably, the amino acid sequence of the complex epitope protein is as shown in SEQ ID NO: as shown at 14.
The invention provides a gene for coding the A-type Seneca virus genetic engineering composite epitope protein, and the nucleotide sequence of the gene is shown as SEQ ID NO: shown at 15.
The invention provides a type-A seneca virus genetic engineering composite epitope protein vaccine, which comprises the type-A seneca virus genetic engineering composite epitope protein and an adjuvant.
Preferably, the concentration of the A-type Selcarinovirus genetic engineering composite epitope protein is 250 mug/mL.
The invention provides a preparation method of the A-type seneca virus genetic engineering composite epitope protein vaccine, which comprises the following steps:
dissolving the A-type seneca virus genetic engineering composite epitope protein by using a PBS buffer solution, mixing with an adjuvant, and emulsifying to obtain the vaccine.
The invention provides a neutralizing antibody of A-type seneca virus, which is obtained by immunizing piglets with the A-type seneca virus genetic engineering composite epitope protein vaccine.
The invention provides application of the A-type Seneca virus genetic engineering composite epitope protein or the neutralizing antibody in preparation of a medicament for preventing and/or controlling porcine A-type Seneca virus diseases or a reagent or a kit for diagnosing the A-type Seneca virus diseases.
The A-type seneca virus genetic engineering composite epitope protein provided by the invention comprises a B cell epitope and a T cell epitope. B cell epitopes of A-type Selenecar virus VP1, VP2 and VP3 proteins are screened, the B cell epitopes of different epitope fragments are matched with one or two universal T cell epitopes to form 3 kinds of composite epitope proteins (rP1, rP2 and rP3), immune piglets are expressed through in-vitro recombination, and remarkable specific antibodies and neutralizing antibodies with different levels can be detected in 28 days; meanwhile, 3 composite epitope proteins (rP1, rP2 and rP3) show different virus attack protection capabilities, wherein the virus attack protection rate of the composite epitope protein rP2 protected by the invention is more than 80%, the virus attack protection rate of the composite epitope protein rP1 is only 40%, and the virus attack protection rate of the composite epitope protein rP3 is only 60%, which shows that the composite epitope protein rP2 protected by the invention has higher virus attack protection effect, is a novel A-type Seneca virus genetic engineering vaccine, and has wide application prospect.
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FIG. 1 is SDS-PAGE analysis of recombinant bacteria expression products; FIG. 1A: recombinant antigen expression following IPTG induction, M: protein molecular weight, 1: non-induced recombinant bacteria control, 2: inducing the recombinant bacteria for 5 hours to obtain a product; FIG. 1B: recombinant antigen expression solubility assay, M: protein molecular weight, 3: recombinant bacteria expression supernatant, 4: expressing and precipitating the recombinant bacteria;
FIG. 2 is SDS-PAGE analysis of recombinant bacteria expressed protein after purification;
FIG. 3 is a Western Blotting detection of recombinant epitope protein; m: protein molecular weight, fig. 3A: the primary antibody is rabbit anti-SVA VP1 polyclonal antibody (diluted by 1:10000), and the secondary antibody is goat anti-rabbit IgG (diluted by 1: 20000); FIG. 3B: the primary antibody is rabbit anti-SVA VP2 polyclonal antibody (diluted by 1:10000), and the secondary antibody is goat anti-rabbit IgG (diluted by 1: 20000); FIG. 3C shows that the primary antibody is porcine SVA positive serum (diluted 1:2000) and the secondary antibody is goat anti-porcine IgG (diluted 1: 10000);
FIG. 4 shows the detection of specific antibody levels in immunized pigs by indirect ELISA.
Detailed Description
The invention provides a gene engineering composite epitope protein of an A-type seneca virus, which comprises a B cell epitope and a T cell epitope.
In the present invention, the B cell epitopes include VP1 protein, VP2 protein and VP3 protein of seneca virus type a.
Wherein fragment 1 in the VP1 protein is 7-26aa on VP1, and the amino acid sequence is shown as SEQ ID NO:1 (TGVIEAGNTDTDFSGELAAP).
Fragment 2 in the VP1 protein is located on VP1 for 48-74aa, and the amino acid sequence is shown as SEQ ID NO:2 (VLEKDAVFPRPFPTATGAQQDDGYFCL).
Fragment 3 in the VP1 protein is positioned on VP1 from 92 aa to 109aa, and the amino acid sequence is shown as SEQ ID NO: 3 (YVNPSDSGVLANTSLDFN).
Fragment 4 in the VP1 protein is located on 183-213 aa of VP1, and the amino acid sequence is shown as SEQ ID NO: 4 (PWNSVSSVLPVRWGGASKLSSATRGLPAHAD).
The VP2 protein comprises a fragment 5-fragment 9.
Wherein, fragment 5 in the VP2 protein is located on VP2 at 12-18aa, and the amino acid sequence is shown as SEQ ID NO:5 (DRVITQT).
The fragment 6 in the VP2 protein is positioned on VP2 from 38 aa to 57aa, and the amino acid sequence is shown as SEQ ID NO: and 6 (EDPTKSDPPSSSTDQPTTTF).
Fragment 7 in the VP2 protein is located at 137-172 aa of VP2, and the amino acid sequence is shown as SEQ ID NO: 7 (PETTLDVKPDGKAKSLQELNEEQWVEMSDDYRTGKNM).
Fragment 8 in the VP2 protein is located at 193-208aa of VP2, and the amino acid sequence is shown as SEQ ID NO: shown at 8 (FINPYQVTVFPHQILN).
Fragment 9 in the VP2 protein is located 249-284aa of VP2, and the amino acid sequence is shown as SEQ ID NO: 9 (KEGATTDPEITFSVRPTSPYFNGLRNRFTTGTDEEQ).
The VP3 protein has an amino acid sequence as shown in SEQ ID NO: 10(AFGRVSEPEPASDAYVPYV), located 55-73 aa above VP 3;
the T cell epitope includes PADRE and invasin. The amino acid sequence of the PADRE is shown as SEQ ID NO:11 (AKFVAAWTLKAAA). The amino acid sequence of the invasin is shown as SEQ ID NO:12 (TAKSKKFPSYTATYQF).
In the present invention, the complex epitope protein preferably links selected B cell epitopes in the order of SVA capsid proteins VP2-VP3-VP1, while linking the polypeptides of T cell epitopes to both ends. The specific connection sequence of the composite epitope protein is preferably PADRE-fragment 5-fragment 6-fragment 7-fragment 8-fragment 9-fragment 10-fragment 1-fragment 2-fragment 3-fragment 4-invasin.
In the present invention, the complex epitope protein preferably further comprises a linker peptide. The B and T cell epitopes are linked by GGC to enhance the correct folding and structural stability of the epitope. The B-B cell epitopes are linked by GGSSGG (SEQ ID NO:13) to ensure that epitope independence is not interfered with. In the embodiment of the invention, the amino acid sequence of the compound epitope protein is shown as SEQ ID NO: as shown at 14.
The preparation method of the A-type Seneca virus genetic engineering composite epitope protein is preferably prepared by an in vitro recombination method. The in vitro recombinant method is not particularly limited in the present invention, and the in vitro recombinant method well known in the art may be used.
The invention provides a gene for coding the A-type Seneca virus genetic engineering composite epitope protein, and the nucleotide sequence of the gene is shown as SEQ ID NO: 15 (gctaaatttgtagcggcatggacactaaaggctgctgctggcggctgcgatcgcgtgatcacccagaccggcggctcgtcaggaggtgaagatccgaccaagagtgacccaccgagcagcagcacggatcagccgacaaccacgtttggcggaagctcgggcggccctgagaccactctggatgtaaaaccggatggcaaggcgaagagcctgcaagaactgaacgaggaacaatgggttgaaatgagcgacgactatcgtacgggcaagaacatgggtggttcgtccggtggttttatcaacccgtaccaagtgaccgtttttccgcaccagattttaaacggtggcagctccggcggcaaagagggtgccacgactgacccggaaattacctttagtgtgcgcccaaccagcccgtactttaacggtctgcgcaatagattcaccaccggtactgacgaagagcaaggtggctcctctggcggggcgttcggtcgtgtcagcgagccggagccggcaagcgacgcgtacgttccgtatgttggtggtagctctggtggcaccggcgttatcgaggcaggtaataccgacaccgacttcagcggtgagctggccgccccgggtggtagctccggtggcgttttggaaaaagatgcggtgttcccgcgtccgtttccgaccgcgacgggtgcgcagcaggatgacggttacttctgcctgggtggctcctcgggcggctacgtgaatccgtccgacaacggcgtcctcgctaataccagcctggatttcaacgggggttcttctgggggtccatggaacagcgtcagctctgtgttgccggttcgttggggcggtgcgagcaaactgagcagcgcaacccgtggtcttccggcgcatgcagatggcgggtgtaccgcgaagtcaaagaaattcccgagctataccgcgacctatcagttc).
The invention provides a type-A seneca virus genetic engineering composite epitope protein vaccine, which comprises the type-A seneca virus genetic engineering composite epitope protein and an adjuvant. The volume ratio of the A-type seneca virus genetic engineering composite epitope protein to the adjuvant is preferably 1: 0.8-1.2, more preferably 1: 1. the concentration of the A-type Selcarinovirus genetic engineering composite epitope protein is preferably 250-300 mug/mL, and more preferably 250 mug/mL. The present invention is not particularly limited in the kind of the adjuvant, and any kind of adjuvants known in the art may be used. In the embodiment of the invention, the adjuvant is preferably Montanide ISA-201 oil adjuvant.
The invention provides a preparation method of the A-type seneca virus genetic engineering composite epitope protein vaccine, which comprises the following steps:
dissolving the A-type seneca virus genetic engineering composite epitope protein by using a PBS buffer solution, mixing with an adjuvant, and emulsifying to obtain the vaccine.
In the invention, after the dissolving, the concentration of the A-type Seneca virus genetic engineering composite epitope protein solution is 500-600 mug/mL, and more preferably 500 mug/mL. The volume ratio of the A-type Selcarinovirus genetic engineering composite epitope protein solution to the adjuvant is preferably 1: 1. The method of emulsification in the present invention is not particularly limited, and emulsification methods known in the art may be used.
The invention also provides a neutralizing antibody of the A-type seneca virus, which is obtained by immunizing piglets by the A-type seneca virus gene engineering composite epitope protein vaccine.
The invention provides application of the A-type Seneca virus genetic engineering composite epitope protein or the neutralizing antibody in preparation of a medicament for preventing and/or controlling porcine A-type Seneca virus.
The dosage form of the drug is not particularly limited in the present invention, and a drug dosage form well known in the art may be used.
The invention provides application of the A-type Seneca virus genetic engineering composite epitope protein or the neutralizing antibody in preparation of a reagent or a kit for diagnosing A-type Seneca virus.
The invention has no special limitation to the types of prepared reagents or kits, and the reagents or kits for detecting the type A epikaavirus disease based on the principle of immunoassay technology, which is well known in the art, can be adopted, such as an enzyme linked immunosorbent assay kit or a colloidal gold assay kit.
The genetically engineered complex epitope protein of Selenecar virus type A, the vaccine and the use thereof provided by the present invention will be described in detail with reference to the following examples, which should not be construed as limiting the scope of the present invention.
Example 1
1. Design of SVA composite epitope gene
Epitopes are the basis of protein antigenicity and are the basic structural and functional units in antigenic molecules that elicit specific immune responses. The epitope which is accurate in positioning and short in amino acid sequence is utilized, the epitope can be effectively identified and presented by an immune system, an organism can be induced to generate specific humoral and cellular immune responses, and the epitope vaccine is used as a genetic engineering vaccine, is strong in specificity, safe, reliable, convenient for large-scale production and has a wide application prospect.
According to literature reports, the SVA VP1 protein has 264 amino acids in length, and comprises 1 BC loop, 1 CD loop and 1 GH loop; the SVA VP2 protein has 284 amino acids in total length, and comprises 1 EF loop, 1 key motif LDV located at 141-143 of VP2 protein and a key motif DGK located at 146-148; the SVA VP3 protein is 239 amino acids in length and contains 1 knob. These structures are associated with viral adsorption, invasion, serotype specificity and protective immune responses, and the antigenic epitopes are mostly located on the loop structures of these capsid proteins. The specific amino acid sequence and position are shown in Table 1.
TABLE 1 position of loop structure on SVA capsid protein and its sequence
Figure BDA0003164615160000071
And as for the screening result of the B cell epitopes of the SVA VP1 protein and the VP2 protein, 3 dominant B cell epitopes are screened from the VP1 protein, which are respectively:
7-26aa：TGVIEAGNTDTDFSGELAAP(SEQ ID NO:1)；
48-74aa：VLEKDAVFPRPFPTATGAQQDDGYFCL(SEQ ID NO:2)；
92-109aa：YVNPSDNGVLANTSLDFN(SEQ ID NO:3)；
7B cell epitopes are screened from the VP2 protein, and are respectively:
1-16aa：DHNTEEMENSADRVIT(SEQ ID NO:20)；
17-32aa：QTAGNTAINTQSSLGV(SEQ ID NO:21；
38-57aa：EDPTKSDPPSSSTDQPTTTF(SEQ ID NO:6)；
137-160aa：PETTLDVKPDGKAKSLQELNEEQW(SEQ ID NO:22)；
154-172aa：ELNEEQWVEMSDDYRTGKN(SEQ ID NO:23)
193-208aa：FINPYQVTVFPHQILN(SEQ ID NO:8)；
249-264aa：EGATTDPEITFSVRP(SEQ ID NO:24)；
265-284aa：TSPYFNGLRNRFTTGTDEEQ(SEQ ID NO:25)。
wherein 38-57aa, 154-172aa, 249-264aa and 265-284aa are dominant B cell epitopes.
According to the screening result of the T cell epitope of the SVA VP1 protein, a T cell epitope may exist in 193-208aa of the VP1 protein. In addition, PADRE (AKFVAAWTLKAAA, SEQ ID NO:11) and invasin (TAKSKKFPSYTATYQF, SEQ ID NO:12) are two general T cell epitopes, and have been widely applied to the design of various epitope vaccines and play a role in enhancing T cell immune response.
Furthermore, according to literature reports, Hui Fan et al co-screen 6B-cell epitopes located on SVA VP1 and VP2 proteins using monoclonal antibody screening, VP 1: 21-26aa, VP2: 12-18aa (SEQ ID NO:5), VP2: 71-76aa, VP2:98-103aa, VP2: 150-156aa and VP2: 248 and 253 aa.
The invention carries out reasonable combination, collocation and connection according to the determined epitope information, and connects the selected B cell epitopes according to the arrangement sequence of the SVA capsid proteins VP4-VP2-VP3-VP1 so as to simulate the original structure of the SVA capsid proteins as much as possible; two lysines (KK) or GGC are connected between the epitopes of the B-T cells to enhance the immunogenicity of the epitopes and maintain the structural stability. The B cell epitopes are linked by GGSSGG, ensuring that epitope independence is not disturbed. 3 groups of SVA composite epitope genes are designed according to the above thought:
rP 1: PADRE-KK-VP2(38-57aa) -VP2(141-148aa) -VP2(154-172aa) -VP2(249-284aa) -VP1(7-26aa) -VP1(48-74aa) -VP1(92-109 aa); the amino acid sequence of the polypeptide is shown as SEQ ID NO. 26:
AKFVAAWTLKAAAKKEDPTKSDPPSSSTDQPTTTFGGSSGGLDVKPDGKGGSSGGELNEEQWVEMSDDYRTGKNGGSSGGKEGATTDPEITFSVRPTSPYFNGLRNRFTTGTDEEQGGSSGGTGVIEAGNTDTDFSGELAAPGGSSGGVLEKDAVFPRPFPTATGAQQDDGYFCLGGSSGGYVNPSDNGVLANTSLDFN；
rP 2: PADRE-GGC-VP2(12-18) -VP2(38-57) -VP2 (137-; the coded amino acid sequence is shown as SEQ ID NO: 14, in the following:
AKFVAAWTLKAAAGGCDRVITQTGGSSGGEDPTKSDPPSSSTDQPTTTFGGSSGGPETTLDVKPDGKAKSLQELNEEQWVEMSDDYRTGKNMGGSSGGFINPYQVTVFPHQILNGGSSGGKEGATTDPEITFSVRPTSPYFNGLRNRFTTGTDEEQGGSSGGAFGRVSEPEPASDAYVPYVGGSSGGTGVIEAGNTDTDFSGELAAPGGSSGGVLEKDAVFPRPFPTATGAQQDDGYFCLGGSSGGYVNPSDNGVLANTSLDFNGGSSGGPWNSVSSVLPVRWGGASKLSSATRGLPAHADGGCTAKSKKFPSYTATYQF。
rP 3: PADRE-KK-VP2(38-57aa) -VP2(141-148aa) -VP2(154-172aa) -VP2(249-284aa) -VP2(249-284aa) -VP2(249-284aa) -VP1(7-26aa) -VP1(48-74aa) -VP1(48-74aa) -VP1(48-74aa) -VP1(92-109aa) -VP1(92-109aa) -VP1(92-109 aa); the amino acid sequence of the polypeptide is shown as SEQ ID NO. 27:
AKFVAAWTLKAAAKKEDPTKSDPPSSSTDQPTTTFGGSSGGLDVKPDGKGGSSGGELNEEQWVEMSDDYRTGKNGGSSGGKEGATTDPEITFSVRPTSPYFNGLRNRFTTGTDEEQGGSSGGKEGATTDPEITFSVRPTSPYFNGLRNRYTTGTDEEQGGSSGGKEGATTDPEITFSVRPTSPYFNGLRNRYKTGTDEEQGGSSGGTGVIEAGNTDTDFSGELAAPGGSSGGVLEKDAVFPRPFPTATGAQQDDGYFCLGGSSGGVLEKDAVFPRPLPTATGAQQDDGYFCLGGSSGGVLEKDAVFPRPFPTATGTQQDDGYFCLGGSSGGYVNPSDSGVLANTSLDFNGGSSGGYVSPSDSGVLANTSLDFNGGSSGGYVSPSDNGVLANTSLDFN。
and (3) carrying out bioinformatics verification analysis on different combined epitope genes to ensure that the target fragment has higher hydrophilicity, antigen index and surface possibility.
Example 2
Expression, purification and identification method of SVA composite epitope protein
Small expression and identification of SVA complex epitope protein: the designed 3 groups of SVA multi-epitope genes are entrusted to Nanjing Kingsry biotech GmbH for codon optimization and synthesis, Nde I enzyme cutting sites and Xho I enzyme cutting sites are respectively added at two ends of the SVA multi-epitope genes, a synthetic sequence is connected to a pET30(a) vector through the Nde I/Xho I enzyme cutting sites for expression of the composite epitope protein, and the recombinant plasmids are named as pET30(a) -rP1, pET30(a) -rP2 and pET30(a) -rP 3. The recombinant plasmid was transformed into BL21(DE3) competent cells for small expression and identification, i.e.: selecting a single colony containing the recombinant plasmid to 5mL of LB liquid medium (kana resistance), culturing overnight at 37 ℃, and preserving the strain at-20 ℃; then selecting single colony containing recombinant plasmid to 5mL LB liquid medium (kana resistance), shake culturing at 37 deg.C to OD600About 0.6; taking part of the bacterial liquid as a control group, adding IPTG inducer (final concentration is 1mM) into the rest bacterial liquid, and performing shake culture at 37 ℃ for 5 hours; two groups of bacteria liquid 0 are respectively taken.15mL, 12000 Xg centrifugation for 2min, bacterial precipitation with 40 u L1X loading buffer heavy suspension lysis, 10ul SDS-PAGE detection. The result is shown in FIG. 1A, the size of the expressed rP1 protein is between 35 kD and 40kD, the size of the expressed rP2 protein is between 40kD and 55kD, and the size of the expressed rP3 protein is between 55kD and 70kD, and the expressed protein is identified as the target protein by mass spectrometry.
Performing large-scale expression and bacterium breaking detection on SVA composite epitope protein: inoculating the overnight culture transformed bacteria identified as positive into 2000mL LB liquid medium at a ratio of 1:1000 until OD is reached600When the value is about 0.6, adding 1mM IPTG (isopropyl thiogalactoside) in final concentration, and performing shake culture at 37 ℃ for 5 hours to obtain recombinant protein after induction expression; centrifuging at 5000rpm for 10min, collecting thallus, washing with precooled PBS twice, and centrifuging at 8000rpm for 10min at 4 deg.C each time. Finally, the thalli is resuspended in PBS with 1/20 volumes, the thalli is ultrasonically crushed on ice, the thalli is centrifuged for 10min at 10000rpm and 4 ℃, and supernatant and sediment are separated; 10ul of the supernatant and the precipitate were each subjected to SDS-PAGE, and the remaining supernatant and the precipitate were kept at 4 ℃ for further use. As shown in FIG. 1B, the 3-histone was mainly expressed in the form of soluble protein in the supernatant.
Purification and concentration determination of SVA complex epitope protein: shaking the collected expression protein supernatant and nickel column packing (Ni-NTA Sefine Resin, BBI) at low speed for 1-2 h at room temperature to make the two generate complete specific combination; after loading the column, the flow-through solution was drained off, and unbound hetero-protein was washed away with 5 column volumes of Binding Buffer. Non-specifically bound heteroproteins were eluted with an Elution buffer containing 40mM imidazole, and the protein of interest was eluted with an Elution buffer containing 500mM imidazole, 5 column volumes per Elution. The collected purified protein was dialyzed, changed to PBS, and then concentrated by ultrafiltration, and the concentration of the purified recombinant protein was measured by using Bradford protein quantitation kit (Biyunshi Co.), and the concentration of the obtained purified rP1 protein was 1.2mg/mL, the concentration of rP2 protein was 1.4mg/mL, and the concentration of rP3 protein was 1.3 mg/mL. The SDS-PAGE analysis of the partially purified rP1, rP2 and rP3 protein solutions shows that the target protein is successfully purified as shown in FIG. 2.
And (3) detecting the antigenicity of the SVA compound epitope protein: the reactivity of the prepared recombinant protein with VP1, VP2 rabbit polyclonal antibody and pig SVA positive serum is detected by a Western blotting method. Carrying out SDS-PAGE electrophoresis on purified recombinant rP1, rP2 and rP3 proteins, transferring the proteins onto an NC membrane, sealing the proteins with a PBST solution containing 5% of milk powder at room temperature for 2h, adding a rabbit anti-SVA-VP 1 polyclonal antibody or a rabbit anti-SVA-VP 2 polyclonal antibody or a pig SVA positive serum (1:2000) diluted by 1:10000, incubating the mixture overnight at 4 ℃, washing the membrane, adding an HRP-labeled goat anti-rabbit IgG or goat anti-pig IgG (1:10000) diluted by 1:20000, incubating the mixture for 1h at room temperature, washing the membrane, adding an ECL color solution for reaction in a dark place, and scanning the NC membrane by a Laser Jet Pro M227fdw MFP imaging system. As a result, the prepared recombinant rP1, rP2 and rP3 proteins showed significant positive reactions with rabbit anti-SVA VP1, VP2 polyclonal antibody and porcine SVA positive serum (see FIG. 3).
Example 3
Vaccine preparation method
The recombinant rP1, rP2 and rP3 proteins prepared in the above example 2 were diluted with PBS to a concentration of 500. mu.g/ml, mixed with an oil adjuvant (Montanide ISA 201 oil adjuvant, Seppic, France) of equal volume and sucked into a 10ml Syringe, and then connected to another Syringe with a double female luer connector (dispersible syline Valve) and injected into each other about 20 times, so that the aqueous phase and the oil phase were mixed well and emulsified into a two-way oil emulsion (W/O/W) vaccine, wherein the final concentrations of rP1, rP2 and rP3 proteins were all 250. mu.g/ml.
Example 4
Animal immunization method
18 healthy and susceptible pigs (pig Seneca virus and foot-and-mouth disease virus antigen negative, and pig Seneca virus serum neutralizing antibody titer is not higher than 1:4) with the same source and variety and the same size of 2 months are taken and randomly divided into 4 groups, 3 negative control groups and 5 rP1, rP2 and rP3 compound epitope protein vaccine immunization groups. Injecting neck muscle, wherein the immunization dose of each pig is 2 ml; the negative control group was injected with an equal amount of PBS. Immunizations were given 1 time on day 0. Jugular vein blood was collected at 0d, 14d, 21d and 28 d. Placing the collected blood at 37 deg.C for 30min, and at 4 deg.C for 2 hr to fully separate out serum; centrifuging at 4000r/min for 10min, carefully aspirating the serum, and detecting SVA-specific antibody, S/P, using a Seika virus indirect ELISA antibody detection kit (purchased from Lanzhou veterinary research institute of Chinese academy of agricultural sciences)The value is more than or equal to 0.4, and the result is judged to be positive; the neutralizing antibody titer of the immune pig serum is detected by a cell neutralization test method. On day 28 post immunization, intramuscular injection (3.0ml, 10) through the neck7.3TCID50) And intranasal inoculation (3.0ml, 1.5ml per nostril, 10)7.3TCID50) The two methods are combined to challenge the virus (SVA/ZJ/2015 strain), and the virus is continuously observed for 10 days after challenge, and the disease condition is recorded in detail. The control pig should have blister on at least 1 hoof, and the immune pig should have blister on any part of the lips and four hooves, i.e. it is judged to be unprotected.
Test results show that 3 groups of recombinant epitope vaccine immunized pigs can detect remarkable SVA specific antibodies on day 14, the S/P value is larger than 0.4, the antibody titer is slowly increased along with the inoculation time, the antibody titer is highest on day 28 of immunization, and the SVA specific antibody titer of an rP3 immunized group is remarkably higher than that of other two groups. Whereas SVA-specific antibodies in PBS-immunized pigs were consistently negative with S/P values <0.4 (see FIG. 4). The detection result of the neutralizing antibody of the test pig serum after 28 days shows that the neutralizing antibody titer of only 1 pig in the rP1 and rP3 immune groups is more than 1:32, the neutralizing antibody titer of the Seneca virus in the rP2 immune group 3/5 pig is more than 1:32, and the neutralizing antibody titer of the PBS control group pig is less than 1: 8; 10 days after challenge, 4 pigs in the rP2 vaccine immunized group resisted SVA challenge and did not exhibit any clinical symptoms. However, only 2 pigs in the rP1 immune group resisted SVA attack, 3 pigs in the rP3 immune group resisted SVA attack, and 3 pigs in the control group (PBS immune group) all attacked within 10 days after virus attack (the results are shown in Table 2). The rP2 composite epitope protein is a good immunogen, and the rP2 composite epitope protein vaccine is a novel, safe and effective Seneca virus vaccine.
TABLE 2 pig immunopotency assay
Figure BDA0003164615160000121
Figure BDA0003164615160000131
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> Lanzhou veterinary research institute of Chinese academy of agricultural sciences
<120> A-type Seneca virus genetic engineering composite epitope protein, vaccine and application thereof
<160> 27
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Thr Gly Val Ile Glu Ala Gly Asn Thr Asp Thr Asp Phe Ser Gly Glu
1 5 10 15
Leu Ala Ala Pro
20
<210> 2
<211> 27
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr Ala Thr
1 5 10 15
Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu
20 25
<210> 3
<211> 18
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Tyr Val Asn Pro Ser Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp
1 5 10 15
Phe Asn
<210> 4
<211> 31
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Pro Trp Asn Ser Val Ser Ser Val Leu Pro Val Arg Trp Gly Gly Ala
1 5 10 15
Ser Lys Leu Ser Ser Ala Thr Arg Gly Leu Pro Ala His Ala Asp
20 25 30
<210> 5
<211> 7
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<213> Artificial Sequence (Artificial Sequence)
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Asp Arg Val Ile Thr Gln Thr
1 5
<210> 6
<211> 20
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Glu Asp Pro Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro
1 5 10 15
Thr Thr Thr Phe
20
<210> 7
<211> 37
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Pro Glu Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu
1 5 10 15
Gln Glu Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg
20 25 30
Thr Gly Lys Asn Met
35
<210> 8
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<213> Artificial Sequence (Artificial Sequence)
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Phe Ile Asn Pro Tyr Gln Val Thr Val Phe Pro His Gln Ile Leu Asn
1 5 10 15
<210> 9
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<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro
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Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr
20 25 30
Asp Glu Glu Gln
35
<210> 10
<211> 19
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<213> Artificial Sequence (Artificial Sequence)
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Ala Phe Gly Arg Val Ser Glu Pro Glu Pro Ala Ser Asp Ala Tyr Val
1 5 10 15
Pro Tyr Val
<210> 11
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<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala
1 5 10
<210> 12
<211> 16
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Thr Ala Lys Ser Lys Lys Phe Pro Ser Tyr Thr Ala Thr Tyr Gln Phe
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<210> 13
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<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Gly Gly Ser Ser Gly Gly
1 5
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<211> 320
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 14
Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Gly Gly Cys
1 5 10 15
Asp Arg Val Ile Thr Gln Thr Gly Gly Ser Ser Gly Gly Glu Asp Pro
20 25 30
Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr
35 40 45
Phe Gly Gly Ser Ser Gly Gly Pro Glu Thr Thr Leu Asp Val Lys Pro
50 55 60
Asp Gly Lys Ala Lys Ser Leu Gln Glu Leu Asn Glu Glu Gln Trp Val
65 70 75 80
Glu Met Ser Asp Asp Tyr Arg Thr Gly Lys Asn Met Gly Gly Ser Ser
85 90 95
Gly Gly Phe Ile Asn Pro Tyr Gln Val Thr Val Phe Pro His Gln Ile
100 105 110
Leu Asn Gly Gly Ser Ser Gly Gly Lys Glu Gly Ala Thr Thr Asp Pro
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Glu Ile Thr Phe Ser Val Arg Pro Thr Ser Pro Tyr Phe Asn Gly Leu
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Arg Asn Arg Phe Thr Thr Gly Thr Asp Glu Glu Gln Gly Gly Ser Ser
145 150 155 160
Gly Gly Ala Phe Gly Arg Val Ser Glu Pro Glu Pro Ala Ser Asp Ala
165 170 175
Tyr Val Pro Tyr Val Gly Gly Ser Ser Gly Gly Thr Gly Val Ile Glu
180 185 190
Ala Gly Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly
195 200 205
Gly Ser Ser Gly Gly Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro
210 215 220
Phe Pro Thr Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu
225 230 235 240
Gly Gly Ser Ser Gly Gly Tyr Val Asn Pro Ser Asp Asn Gly Val Leu
245 250 255
Ala Asn Thr Ser Leu Asp Phe Asn Gly Gly Ser Ser Gly Gly Pro Trp
260 265 270
Asn Ser Val Ser Ser Val Leu Pro Val Arg Trp Gly Gly Ala Ser Lys
275 280 285
Leu Ser Ser Ala Thr Arg Gly Leu Pro Ala His Ala Asp Gly Gly Cys
290 295 300
Thr Ala Lys Ser Lys Lys Phe Pro Ser Tyr Thr Ala Thr Tyr Gln Phe
305 310 315 320
<210> 15
<211> 960
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
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gctaaatttg tagcggcatg gacactaaag gctgctgctg gcggctgcga tcgcgtgatc 60
acccagaccg gcggctcgtc aggaggtgaa gatccgacca agagtgaccc accgagcagc 120
agcacggatc agccgacaac cacgtttggc ggaagctcgg gcggccctga gaccactctg 180
gatgtaaaac cggatggcaa ggcgaagagc ctgcaagaac tgaacgagga acaatgggtt 240
gaaatgagcg acgactatcg tacgggcaag aacatgggtg gttcgtccgg tggttttatc 300
aacccgtacc aagtgaccgt ttttccgcac cagattttaa acggtggcag ctccggcggc 360
aaagagggtg ccacgactga cccggaaatt acctttagtg tgcgcccaac cagcccgtac 420
tttaacggtc tgcgcaatag attcaccacc ggtactgacg aagagcaagg tggctcctct 480
ggcggggcgt tcggtcgtgt cagcgagccg gagccggcaa gcgacgcgta cgttccgtat 540
gttggtggta gctctggtgg caccggcgtt atcgaggcag gtaataccga caccgacttc 600
agcggtgagc tggccgcccc gggtggtagc tccggtggcg ttttggaaaa agatgcggtg 660
ttcccgcgtc cgtttccgac cgcgacgggt gcgcagcagg atgacggtta cttctgcctg 720
ggtggctcct cgggcggcta cgtgaatccg tccgacaacg gcgtcctcgc taataccagc 780
ctggatttca acgggggttc ttctgggggt ccatggaaca gcgtcagctc tgtgttgccg 840
gttcgttggg gcggtgcgag caaactgagc agcgcaaccc gtggtcttcc ggcgcatgca 900
gatggcgggt gtaccgcgaa gtcaaagaaa ttcccgagct ataccgcgac ctatcagttc 960
<210> 16
<211> 25
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr Ala Thr
1 5 10 15
Gly Ala Gln Gln Asp Asp Gly Tyr Phe
20 25
<210> 17
<211> 16
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 17
Tyr Val Asn Pro Ser Asp Asn Gly Val Leu Ala Asn Thr Ser Leu Asp
1 5 10 15
<210> 18
<211> 29
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Gly Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro Pro
1 5 10 15
Asn Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln
20 25
<210> 19
<211> 17
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
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Ala Phe Gly Arg Val Ser Glu Pro Glu Pro Ala Ser Asp Ala Tyr Val
1 5 10 15
Pro
<210> 20
<211> 16
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 20
Asp His Asn Thr Glu Glu Met Glu Asn Ser Ala Asp Arg Val Ile Thr
1 5 10 15
<210> 21
<211> 16
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 21
Gln Thr Ala Gly Asn Thr Ala Ile Asn Thr Gln Ser Ser Leu Gly Val
1 5 10 15
<210> 22
<211> 24
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 22
Pro Glu Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu
1 5 10 15
Gln Glu Leu Asn Glu Glu Gln Trp
20
<210> 23
<211> 19
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 23
Glu Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg Thr
1 5 10 15
Gly Lys Asn
<210> 24
<211> 15
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 24
Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro
1 5 10 15
<210> 25
<211> 20
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 25
Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr
1 5 10 15
Asp Glu Glu Gln
20
<210> 26
<211> 199
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 26
Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Lys Lys Glu
1 5 10 15
Asp Pro Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr
20 25 30
Thr Thr Phe Gly Gly Ser Ser Gly Gly Leu Asp Val Lys Pro Asp Gly
35 40 45
Lys Gly Gly Ser Ser Gly Gly Glu Leu Asn Glu Glu Gln Trp Val Glu
50 55 60
Met Ser Asp Asp Tyr Arg Thr Gly Lys Asn Gly Gly Ser Ser Gly Gly
65 70 75 80
Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro
85 90 95
Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr
100 105 110
Asp Glu Glu Gln Gly Gly Ser Ser Gly Gly Thr Gly Val Ile Glu Ala
115 120 125
Gly Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Gly
130 135 140
Ser Ser Gly Gly Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe
145 150 155 160
Pro Thr Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Gly
165 170 175
Gly Ser Ser Gly Gly Tyr Val Asn Pro Ser Asp Asn Gly Val Leu Ala
180 185 190
Asn Thr Ser Leu Asp Phe Asn
195
<210> 27
<211> 397
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 27
Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Lys Lys Glu
1 5 10 15
Asp Pro Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr
20 25 30
Thr Thr Phe Gly Gly Ser Ser Gly Gly Leu Asp Val Lys Pro Asp Gly
35 40 45
Lys Gly Gly Ser Ser Gly Gly Glu Leu Asn Glu Glu Gln Trp Val Glu
50 55 60
Met Ser Asp Asp Tyr Arg Thr Gly Lys Asn Gly Gly Ser Ser Gly Gly
65 70 75 80
Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro
85 90 95
Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr
100 105 110
Asp Glu Glu Gln Gly Gly Ser Ser Gly Gly Lys Glu Gly Ala Thr Thr
115 120 125
Asp Pro Glu Ile Thr Phe Ser Val Arg Pro Thr Ser Pro Tyr Phe Asn
130 135 140
Gly Leu Arg Asn Arg Tyr Thr Thr Gly Thr Asp Glu Glu Gln Gly Gly
145 150 155 160
Ser Ser Gly Gly Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe
165 170 175
Ser Val Arg Pro Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Tyr
180 185 190
Lys Thr Gly Thr Asp Glu Glu Gln Gly Gly Ser Ser Gly Gly Thr Gly
195 200 205
Val Ile Glu Ala Gly Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala
210 215 220
Ala Pro Gly Gly Ser Ser Gly Gly Val Leu Glu Lys Asp Ala Val Phe
225 230 235 240
Pro Arg Pro Phe Pro Thr Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr
245 250 255
Phe Cys Leu Gly Gly Ser Ser Gly Gly Val Leu Glu Lys Asp Ala Val
260 265 270
Phe Pro Arg Pro Leu Pro Thr Ala Thr Gly Ala Gln Gln Asp Asp Gly
275 280 285
Tyr Phe Cys Leu Gly Gly Ser Ser Gly Gly Val Leu Glu Lys Asp Ala
290 295 300
Val Phe Pro Arg Pro Phe Pro Thr Ala Thr Gly Thr Gln Gln Asp Asp
305 310 315 320
Gly Tyr Phe Cys Leu Gly Gly Ser Ser Gly Gly Tyr Val Asn Pro Ser
325 330 335
Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn Gly Gly Ser
340 345 350
Ser Gly Gly Tyr Val Ser Pro Ser Asp Ser Gly Val Leu Ala Asn Thr
355 360 365
Ser Leu Asp Phe Asn Gly Gly Ser Ser Gly Gly Tyr Val Ser Pro Ser
370 375 380
Asp Asn Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn
385 390 395

Claims (10)

1. A type Seleneka virus genetic engineering composite epitope protein is characterized by comprising a B cell epitope and a T cell epitope;
the B cell epitope comprises VP1 protein, VP2 protein and VP3 protein of the type A seneca virus; the VP1 protein comprises an amino acid sequence shown as SEQ ID NO:1 to SEQ ID NO: 4, fragment 1 to fragment 4;
the VP2 protein comprises an amino acid sequence shown as SEQ ID NO:5 to SEQ ID NO: 9, segment 5 to segment 9;
the VP3 protein comprises an amino acid sequence shown as SEQ ID NO: 10, segment 10;
the T cell epitope comprises PADRE and invasin;
the amino acid sequence of the PADRE is shown as SEQ ID NO:11 is shown in the figure;
the amino acid sequence of the invasin is shown as SEQ ID NO: shown at 12.
2. The A-type Selenecar virus genetic engineering composite epitope protein according to claim 1, wherein the composite epitope protein is PADRE-fragment 5-fragment 6-fragment 7-fragment 8-fragment 9-fragment 10-fragment 1-fragment 2-fragment 3-fragment 4-invasin.
3. The type a seneca virus genetically engineered composite epitope protein of claim 1, further comprising a linker peptide;
the B cell epitopes are connected by a connecting peptide GGSSGG; the B cell epitope and the T cell epitope are connected by a connecting peptide GGC.
4. The A-type Seneca virus genetically engineered composite epitope protein of claim 3, wherein the amino acid sequence of said composite epitope protein is as set forth in SEQ ID NO: as shown at 14.
5. A gene for coding the A-type Seneca virus genetic engineering composite epitope protein of any one of claims 1 to 4, characterized in that the nucleotide sequence of the gene is shown as SEQ ID NO: shown at 15.
6. A type Selenecar virus genetic engineering composite epitope protein vaccine, which is characterized by comprising the A type Selenecar virus genetic engineering composite epitope protein of any one of claims 1-4 and an adjuvant.
7. The A-type Selenecar virus genetically engineered composite epitope protein vaccine of claim 6, wherein the concentration of the A-type Selenecar virus genetically engineered composite epitope protein is 250 μ g/mL.
8. The method for preparing the A-type seneca virus genetic engineering composite epitope protein vaccine of claim 6 or 7, which is characterized by comprising the following steps:
dissolving the A-type seneca virus genetic engineering composite epitope protein by using a PBS buffer solution, mixing with an adjuvant, and emulsifying to obtain the vaccine.
9. A neutralizing antibody of the A-type Selenecar virus, which is obtained by immunizing piglets with the A-type Selenecar virus genetic engineering composite epitope protein vaccine of claim 6 or 7.
10. Use of the A-type Seneca virus genetically engineered complex epitope protein of any one of claims 1 to 4 or the neutralizing antibody of claim 9 in the preparation of a medicament for preventing and/or controlling porcine A-type Seneca virus disease or in the preparation of a reagent or kit for diagnosing A-type Seneca virus disease.
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