CN112931123B - Method for relieving phytotoxicity of atrazine on soybeans by utilizing rhizobia inoculation - Google Patents
Method for relieving phytotoxicity of atrazine on soybeans by utilizing rhizobia inoculation Download PDFInfo
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- CN112931123B CN112931123B CN202110424106.4A CN202110424106A CN112931123B CN 112931123 B CN112931123 B CN 112931123B CN 202110424106 A CN202110424106 A CN 202110424106A CN 112931123 B CN112931123 B CN 112931123B
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- rhizobia
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- atrazine
- phytotoxicity
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- 244000068988 Glycine max Species 0.000 title claims abstract description 74
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 69
- MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000011081 inoculation Methods 0.000 title claims abstract description 21
- 231100000674 Phytotoxicity Toxicity 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 63
- 239000002689 soil Substances 0.000 claims abstract description 60
- 230000001580 bacterial effect Effects 0.000 claims abstract description 33
- 241000589180 Rhizobium Species 0.000 claims abstract description 15
- 238000012258 culturing Methods 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000009630 liquid culture Methods 0.000 claims description 4
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 4
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 3
- 230000036512 infertility Effects 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 14
- 230000012010 growth Effects 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000003213 activating effect Effects 0.000 abstract 1
- 238000002347 injection Methods 0.000 description 14
- 239000007924 injection Substances 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000024121 nodulation Effects 0.000 description 4
- 241000589174 Bradyrhizobium japonicum Species 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010063653 Leghemoglobin Proteins 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000003828 downregulation Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000008832 photodamage Effects 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004178 biological nitrogen fixation Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/40—Fabaceae, e.g. beans or peas
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for relieving phytotoxicity of atrazine on soybeans by utilizing rhizobia inoculation, and relates to the field of agricultural microorganism application. The invention aims to solve the technical problem that the existing black soil region has serious phytotoxicity on soybean caused by residual atrazine. The method comprises the following steps: activating and culturing rhizobium strains to prepare rhizobium bacterial liquid; planting soybean seeds, after the soybean seedlings grow out of the first pair of true leaves, injecting the rhizobia bacterial liquid in the step one around the roots of the soybean seedlings, and continuously culturing. After rhizobia is applied by the method, the stress effect of atrazine on soybean growth can be obviously relieved. The invention is used for relieving the phytotoxicity of atrazine on soybean.
Description
Technical Field
The invention relates to the field of agricultural microorganism application.
Background
Corn and soybean rotation is a sustainable planting mode widely applied in northeast China, and can effectively maintain soil fertility. However, in actual production, continuous cropping of corn throughout the year, excessive application of herbicide results in higher and higher residual atrazine in soil and serious damage to soybean in the next crop. Soybeans are crops which are sensitive to atrazine stress, and a nitrogen fixation system formed by leguminous plants and root nodules has great significance for the ecological system of the earth and agricultural production. The biological nitrogen fixation function of the soybean is fully exerted by inoculating rhizobia in a large area abroad, so that obvious economic benefit and ecological benefit are obtained. However, nitrogen fertilizer is commonly applied in soybean production in China, which not only reduces the fertilizer utilization rate, but also directly increases the production cost. Therefore, the method for popularizing rhizobia and optimizing the adding mode of rhizobia has wide prospect in the soybean production in black soil areas of China. However, a rhizobia application method aiming at relieving phytotoxicity of atrazine remained in black soil areas is not available at present.
Disclosure of Invention
The invention aims to solve the technical problem that the existing black soil region has serious phytotoxicity effect of residual atrazine on soybean, and provides a method for relieving the phytotoxicity effect of atrazine on soybean by utilizing rhizobia inoculation.
A method for alleviating the phytotoxicity of atrazine on soybeans by utilizing rhizobia inoculation is carried out according to the following steps:
1. transferring rhizobium strain to YMA plane culture medium for activation, then picking single colony, inoculating in YMA liquid culture medium for culture to obtain rhizobium bacterial liquid;
2. planting soybean seeds in the soil polluted by the altrette, injecting the rhizobia liquid in the step one around the root of the soybean seedling after the soybean seedling grows out of the first pair of true leaves, and continuously culturing to finish the method.
Further, the rhizobia in the first step is a strain Bradyrhizobium japonicum AC, which is purchased from Shanghai collection biotechnology center and has a collection number of SHBCC D11101.
The beneficial effects of the invention are as follows:
after rhizobia is applied by the method, the stress effect of atrazine on soybean growth is obviously relieved. The atrazine in the polluted soil has obvious inhibition effect on the growth and nodulation of soybean, and the strain AC20 (1.2X10) 7 CFU/mL～1.2×10 9 CFU/mL), the inhibiting effect of atrazine is obviously relieved, and when the adding amount is 1.2X10 8 ～1.2×10 9 CFU/mL mitigationThe effect is most obvious. In addition, the atrazine can also obviously inhibit the nodulation number of the soybean, and the soybean hemoglobin content is reduced from 9.1mg/g to 3.4mg/g in the aspects of root nodule number reduction, dry weight down regulation and the like; according to the invention, the rhizobia added with the AC20 can effectively relieve the inhibition effect of atrazine on the indexes, and the content of leghemoglobin is increased by 111.4% through verification, meanwhile, the photosynthetic pigment content and the nitrogen accumulation amount of soybean plants under the stress of atrazine can be effectively increased through rhizobia inoculation, the corresponding contents are respectively increased by 87.23% and 61.85% compared with a treatment group without adding strains, and the soybean photodamage and nutrient loss caused by the stress of atrazine are relieved.
The invention is used for relieving the phytotoxicity of atrazine on soybean.
Drawings
FIG. 1 is a comparative photograph of soybean plant growth in example, 1 represents uncontaminated soil, uninjected rhizobia liquid, 2 represents contaminated soil with atrazine concentration of 20mg.kg -1 3 represents that the concentration of atrazine in the polluted soil is 20mg.kg without being injected with rhizobia bacteria liquid -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 9 CFU/mL；
FIG. 2 is a graph showing the effect of rhizobia inoculation on soybean plant weight (dry weight) in example, wherein a represents uncontaminated soil, uncontaminated rhizobia liquid, and b represents uncontaminated soil, and the effective viable count in the perfused rhizobia liquid is 1.2X10 7 CFU/mL, c represents the uncontaminated soil, and the effective viable count of the injected rhizobia bacterial liquid is 1.2X10 8 CFU/mL, d represents the effective viable count of 1.2X10 in uncontaminated soil and injected rhizobia bacterial liquid 9 CFU/mL, e represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 F represents that the concentration of atrazine in polluted soil is 20mg.kg without being injected with rhizobia bacteria liquid -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 7 CFU/mL, g represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 8 CFU/mL, h represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 9 CFU/mL；
FIG. 3 is a graph showing the effect of rhizobia inoculation on soybean root weight (dry weight) in example, wherein a represents uncontaminated soil, uncontaminated rhizobia liquid, and b represents uncontaminated soil, and the effective viable count in the reinjection rhizobia liquid is 1.2X10 7 CFU/mL, c represents the uncontaminated soil, and the effective viable count of the injected rhizobia bacterial liquid is 1.2X10 8 CFU/mL, d represents the effective viable count of 1.2X10 in uncontaminated soil and injected rhizobia bacterial liquid 9 CFU/mL, e represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 F represents that the concentration of atrazine in polluted soil is 20mg.kg without being injected with rhizobia bacteria liquid -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 7 CFU/mL, g represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 8 CFU/mL, h represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 9 CFU/mL。
Detailed Description
The technical scheme of the invention is not limited to the specific embodiments listed below, but also includes any combination of the specific embodiments.
The first embodiment is as follows: the method for alleviating the phytotoxicity of atrazine on soybeans by utilizing rhizobia inoculation in the embodiment comprises the following steps:
1. transferring rhizobium strain to YMA plane culture medium for activation, then picking single colony, inoculating in YMA liquid culture medium for culture to obtain rhizobium bacterial liquid;
2. planting soybean seeds in the soil polluted by the altrette, injecting the rhizobia liquid in the step one around the root of the soybean seedling after the soybean seedling grows out of the first pair of true leaves, and continuously culturing to finish the method.
The second embodiment is as follows: the first difference between this embodiment and the specific embodiment is that: step one, the rhizobia is a strain Bradyrhizobium japonicum AC, and is purchased from Shanghai collection biotechnology center, and the collection number is SHBCC D11101. The other is the same as in the first embodiment.
And a third specific embodiment: this embodiment differs from the first or second embodiment in that: step one, adopting a dilution or concentration method to adjust the effective viable count in the rhizobium bacterial liquid to be 1.2 multiplied by 10 7 ～1.2×10 9 CFU/mL. The other is the same as the first or second embodiment.
The specific embodiment IV is as follows: this embodiment differs from one of the first to third embodiments in that: the effective viable count in the rhizobia bacterial liquid in the step one is 1.2 multiplied by 10 8 CFU/mL. The other is the same as in one of the first to third embodiments.
Fifth embodiment: this embodiment differs from one to four embodiments in that: and step two, the concentration of the altrette in the soil is lower than 20mg/kg. The others are the same as in one to one fourth embodiments.
Specific embodiment six: this embodiment differs from one of the first to fifth embodiments in that: and step two, performing sterilization treatment on the soybean seeds in advance, and then planting. The others are the same as in one of the first to fifth embodiments.
Seventh embodiment: this embodiment differs from one of the first to sixth embodiments in that: the sterilization treatment of soybean seeds specifically comprises: sterilizing soybean seeds in sodium hypochlorite solution with mass concentration of 3% for 3min, and washing with distilled water for 3 times to ensure surface sterility. The others are the same as in one of the first to sixth embodiments.
Eighth embodiment: this embodiment differs from one of the first to seventh embodiments in that: and step two, the volume of rhizobia liquid injected into each soybean seedling is 1mL. The other is the same as in one of the first to seventh embodiments.
Detailed description nine: this embodiment differs from one to eight of the embodiments in that: injecting rhizobium liquid into the rhizosphere soil surface of the seedling. The others are the same as in one to eight embodiments.
Detailed description ten: this embodiment differs from one of the embodiments one to nine in that: and step two, after the rhizobia bacterial liquid is injected, culturing is continued for 25 days. The others are the same as in one of the embodiments one to nine.
The following examples are used to verify the benefits of the present invention:
embodiment one:
the method for alleviating the phytotoxicity of atrazine on soybeans by utilizing rhizobia inoculation in the embodiment comprises the following steps:
1. transferring rhizobium strain to YMA plane culture medium for activation, then picking single colony, inoculating in YMA liquid culture medium for culture to obtain rhizobium bacterial liquid;
2. air-dried soil and vermiculite are fully mixed according to the mass ratio of 3:1, and atrazine is added and fully mixed to prepare polluted soil, wherein the initial concentration of atrazine in the polluted soil is 20mg.kg -1 (dry weight); loading the polluted soil into flowerpots (length x width x height=10 cm x 10cm x 8.5 cm) with three holes at the bottom, and pouring 200g of polluted soil into each flowerpot to 60% of the field water holding capacity by using distilled water for later use;
3. sterilizing soybean seeds in sodium hypochlorite (NaClO) solution with mass concentration of 3% for 3min, washing with distilled water for 3 times to ensure surface sterility, culturing sterilized soybean seeds in a constant temperature incubator with filter paper at 30deg.C for 24h in dark place, transferring germinated seeds into flowerpots filled with polluted soil, and transferring 9 soybean seeds into each flowerpots; adding a certain amount of deionized water, and culturing in an illumination culture room at 25 ℃ and 18 ℃ respectively; after the first pair of true leaves (about 10 d) of the soybean seedlings were grown, the rhizobia liquid (od600=0.6, 1.2x10 8 cfu/mL); the volume of the rhizobia liquid injected into each soybean strain is 1mL, and the soybean strain is continuously cultured for 25 days, and relevant indexes are sampled and measured.
Step one, the rhizobia is a strain Bradyrhizobium japonicum AC, and is purchased from Shanghai collection biotechnology center, and the collection number is SHBCC D11101.
The effective viable count in the rhizobia bacterial liquid in the step one is 1.2 multiplied by 10 8 CFU/mL。
Injecting rhizobium liquid into the rhizosphere soil surface of the seedling.
Embodiment two:
the first difference between this embodiment and the first embodiment is that: the effective viable count of rhizobia liquid injected into the periphery of root portion of soybean seedling is 1.2X10 7 CFU/mL。
Embodiment III:
the first difference between this embodiment and the first embodiment is that: the effective viable count of rhizobia liquid injected into the periphery of root portion of soybean seedling is 1.2X10 9 CFU/mL。
FIG. 1 is a comparative photograph of soybean plant growth in example, 1 represents uncontaminated soil, uninjected rhizobia liquid, 2 represents contaminated soil with atrazine concentration of 20mg.kg -1 3 represents that the concentration of atrazine in the polluted soil is 20mg.kg without being injected with rhizobia bacteria liquid -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 9 CFU/mL. FIG. 2 is a graph showing the effect of rhizobia inoculation on soybean plant weight (dry weight) in example, wherein a represents uncontaminated soil, uncontaminated rhizobia liquid, and b represents uncontaminated soil, and the effective viable count in the perfused rhizobia liquid is 1.2X10 7 CFU/mL, c represents the uncontaminated soil, and the effective viable count of the injected rhizobia bacterial liquid is 1.2X10 8 CFU/mL, d represents the effective viable count of 1.2X10 in uncontaminated soil and injected rhizobia bacterial liquid 9 CFU/mL, e represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 F represents that the concentration of atrazine in polluted soil is 20mg.kg without being injected with rhizobia bacteria liquid -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 7 CFU/mL, g represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 8 CFU/mL, h represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 9 CFU/mL。
FIG. 3 is a graph showing the effect of rhizobia inoculation on soybean root weight (dry weight) in example, wherein a represents uncontaminated soil, uncontaminated rhizobia liquid, and b represents uncontaminated soil, and the effective viable count in the reinjection rhizobia liquid is 1.2X10 7 CFU/mL, c represents the uncontaminated soil, and the effective viable count of the injected rhizobia bacterial liquid is 1.2X10 8 CFU/mL, d represents the effective viable count of 1.2X10 in uncontaminated soil and injected rhizobia bacterial liquid 9 CFU/mL, e represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 F represents that the concentration of atrazine in polluted soil is 20mg.kg without being injected with rhizobia bacteria liquid -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 7 CFU/mL, g represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 8 CFU/mL, h represents that the concentration of atrazine in the polluted soil is 20mg.kg -1 The effective viable count of the injection rhizobia bacterial liquid is 1.2X10 9 CFU/mL。
From the above figures, it is concluded that: atrazine (20 mg/kg) significantly inhibited the growth of soybean plants, strain AC20 (1.2X10) 7 CFU/mL～1.2×10 9 CFU/mL), the dry biomass of the soybeans is obviously increased, and the stress effect of atrazine on the growth of the soybeans is effectively relieved; the number of effective live bacteria is 1.2 multiplied by 10 9 At CFU/mL, plant dry matter content peaks.
The atrazine in the polluted soil has proved to have remarkable inhibition effect on the growth and nodulation of soybean, and rhizobia AC20 (the addition amount is 1.2X10) 7 CFU/mL～1.2×10 9 CFU/mL), the inhibition of atrazine is significantly reduced. In addition, the atrazine can also obviously inhibit the nodulation number of the soybean, and the soybean hemoglobin content is reduced from 9.1mg/g to 3.4mg/g in the aspects of root nodule number reduction, dry weight down regulation and the like; according to the embodiment, the rhizobia added with the AC20 can effectively relieve the inhibition effect of atrazine on the indexes, and through verification, the content of leghemoglobin is increased by 111.4%, meanwhile, the photosynthetic pigment content and the nitrogen accumulation amount of soybean plants under the stress of atrazine can be effectively increased through rhizobia inoculation, the corresponding contents are respectively increased by 87.23% and 61.85% compared with a treatment group without adding strains, and the soybean photodamage and nutrient loss caused by the stress of atrazine are relieved.
Claims (8)
1. A method for alleviating the phytotoxicity of atrazine on soybeans by utilizing rhizobia inoculation is characterized by comprising the following steps:
1. transferring rhizobium strain to YMA plane culture medium for activation, then picking single colony, inoculating in YMA liquid culture medium for culture to obtain rhizobium bacterial liquid;
2. planting soybean seeds in the soil polluted by the altrette, injecting rhizobia liquid in the step one around the roots of the soybean seedlings after the soybean seedlings grow out of a first pair of true leaves, and continuously culturing to finish the method;
step one, the rhizobium is a strainBradyrhizobium japonicum AC20, available from the Shanghai collection biotechnology center under the collection number SHBCC D11101;
step one, adopting a dilution or concentration method to adjust the effective viable count in the rhizobium bacterial liquid to be 1.2 multiplied by 10 7 ~1.2×10 9 CFU /mL。
2. The method for alleviating the phytotoxicity of atrazine on soybean by rhizobia inoculation according to claim 1, wherein the effective viable count in the rhizobia liquid in step one is 1.2X10 8 CFU /mL。
3. A method of alleviating the phytotoxicity of atrazine on soybean by means of rhizobia inoculation according to claim 1, wherein the concentration of atrazine in the soil in step two is less than 20mg/kg.
4. The method for alleviating the phytotoxicity of atrazine on soybean by rhizobia inoculation according to claim 1, wherein in step two, the soybean seeds are sterilized in advance and planted.
5. The method for alleviating the phytotoxicity of atrazine on soybean by rhizobia inoculation according to claim 4, wherein the sterilization treatment of the soybean seeds is specifically: sterilizing soybean seeds in sodium hypochlorite solution with mass concentration of 3% for 3min, and washing with distilled water for 3 times to ensure surface sterility.
6. The method for alleviating the phytotoxicity of atrazine on soybean by rhizobia inoculation according to claim 1, wherein the volume of rhizobia liquid injected into each soybean seedling in step two is 1mL.
7. The method for alleviating the phytotoxicity of atrazine on soybean by means of rhizobia inoculation according to claim 1, wherein the step of injecting the rhizobia liquid into the rhizosphere soil surface of the seedling.
8. The method for alleviating the phytotoxicity of atrazine on soybean by rhizobia inoculation according to claim 1, wherein the culturing is continued for a further 25 days after the step two of injecting the rhizobia liquid.
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|---|---|---|---|---|
| JP2005027536A (en) * | 2003-07-09 | 2005-02-03 | National Institute For Agro-Environmental Science | Method for degrading agrochemical with new agrochemical-degrading bacterium or compound microorganism system |
| CN105713857A (en) * | 2016-03-01 | 2016-06-29 | 同济大学 | Atrazine degrading bacterium and application thereof |
| CN110668876A (en) * | 2019-11-05 | 2020-01-10 | 内蒙古农业大学 | Composite mycorrhiza biological fertilizer and preparation method and application thereof |
| CN112189525A (en) * | 2020-10-09 | 2021-01-08 | 四川农业大学 | Method for promoting leguminous plant growth by using arbuscular mycorrhizal fungi and rhizobia |
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- 2021-04-20 CN CN202110424106.4A patent/CN112931123B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005027536A (en) * | 2003-07-09 | 2005-02-03 | National Institute For Agro-Environmental Science | Method for degrading agrochemical with new agrochemical-degrading bacterium or compound microorganism system |
| CN105713857A (en) * | 2016-03-01 | 2016-06-29 | 同济大学 | Atrazine degrading bacterium and application thereof |
| CN110668876A (en) * | 2019-11-05 | 2020-01-10 | 内蒙古农业大学 | Composite mycorrhiza biological fertilizer and preparation method and application thereof |
| CN112189525A (en) * | 2020-10-09 | 2021-01-08 | 四川农业大学 | Method for promoting leguminous plant growth by using arbuscular mycorrhizal fungi and rhizobia |
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| Title |
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