CN112924419A - Light source component and specific protein analysis system - Google Patents

Abstract

The invention provides a light source component applied to a specific protein analysis system, which comprises a laser, an aperture diaphragm and a collimating lens, wherein the aperture diaphragm is arranged between the laser and the collimating lens, the aperture diaphragm is provided with a first light through hole which penetrates through the aperture diaphragm, laser emitted by the laser passes through the first light through hole, is collimated by the collimating lens and is emitted backwards, and the axis of the first light through hole is not coincident with the optical axis of the collimating lens so as to prevent the laser reflected by the collimating lens from reflecting back to the laser. According to the light source assembly, the light axis is arranged, so that the influence of reflected light on the detection light source emitted by the laser can be limited, and the quality of the detection light source is improved. The invention also relates to a specific protein analysis system also for reducing the influence of reflected light on a detection light source.

Description

Light source component and specific protein analysis system

Technical Field

The invention relates to the technical field of medical detection, in particular to a light source assembly and a specific protein analysis system.

Background

Markers such as the number of leukocytes, erythrocyte sedimentation rate, acute phase proteins, etc. can be used to observe signs of inflammation and the degree of inflammation in humans. Among them, C-reactive protein (CRP), which is one of acute-phase proteins, rapidly increases in blood concentration after being stimulated by bacterial infection, inflammation, or surgery. Therefore, the method has wider application in clinic. The detection range of the common C-reactive protein kit is generally 3-200mg/L, the lowest detection limit is generally 3-5mg/L, but the defect of low sensitivity exists. Especially for pediatric interference diseases, as the content of C-reactive protein of a common newborn is low, the conventional detection reaction can not produce tiny change; in the aspect of cardiovascular disease diagnosis, CRP <1mg/L is low risk, CRP 1-3mg/L is medium risk, and CRP >3mg/L is high risk. Therefore, a CRP concentration detection method with lower detection line and higher detection precision is urgently needed clinically.

The common high-precision CRP detection method at present is a whole blood CRP detection method. The detection method is based on a latex scattering method, after a sample is hemolyzed, when an antigen in the sample meets latex particles adsorbed with antibodies, the antigen and the antibodies are combined to generate latex agglutination. The size of the individual latex particles is within the wavelength of the incident light and allows light to pass through. When two or more latex particles are aggregated, light transmission is hindered, transmitted light is reduced, and scattered light is increased. The degree of change in transmitted light or scattered light is proportional to the degree of latex agglutination and also proportional to the amount of antigen in the sample, and the amount of antigen in the sample can be determined by measuring the absorbance or scattered light of the sample.

However, the main defects of the coherent light turbidimetric detection system in the market at present are as follows: components such as a collimating lens and the like in the detection system can reflect light rays, and if the reflected light enters the laser, an external field interference effect of the laser can be triggered, so that the output power and the output wavelength of the laser are unstable, and the system measurement accuracy of the detection device is influenced.

Disclosure of Invention

Accordingly, the present invention provides a light source assembly for a specific protein analysis system to reduce the influence of reflected light on the detection light source. The invention also relates to a specific protein analysis system, which is also used for reducing the influence of reflected light on a detection light source.

In a first aspect, the light source assembly applied to a specific protein analysis system provided by the invention comprises a laser, an aperture diaphragm and a collimating lens, wherein the aperture diaphragm is arranged between the laser and the collimating lens, the aperture diaphragm is provided with a first light through hole penetrating through the aperture diaphragm, laser emitted by the laser passes through the first light through hole, is collimated by the collimating lens and is emitted backwards, and an axis of the first light through hole is not coincident with an optical axis of the collimating lens, so that the laser reflected by the collimating lens is prevented from being reflected back to the laser.

Wherein, the axis of the first light through hole intersects with the optical axis of the collimating lens to form a first included angle.

Wherein the first included angle α satisfies: alpha is more than or equal to 3 degrees and less than or equal to 25 degrees.

The collimating lens comprises a light incident surface close to the small-hole diaphragm, the light incident surface is a curved surface, and the axis of the first light passing hole is parallel to the optical axis of the collimating lens and forms a specific distance.

Wherein the specific distance d satisfies: d is not less than D1/4 and not more than D1/100, wherein D1 is the diameter of the collimating lens.

The aperture diaphragm is made of light absorption materials, or the aperture diaphragm is provided with a light absorption film layer.

An isolator is arranged between the small aperture diaphragm and the collimating lens along the axis of the first light through hole, and is used for blocking laser reflected by the collimating lens.

The collimating lens comprises a light incident surface close to the aperture diaphragm, and an antireflection film for reducing the light reflectivity of the light incident surface is arranged on the light incident surface.

The collimating lens further comprises a light emergent surface opposite to the light incident surface, and an antireflection film for reducing the light reflectivity of the light incident surface is also arranged on the light emergent surface.

The light source assembly further comprises a light spot limiting diaphragm used for controlling the size of the light spot, the light spot limiting diaphragm is arranged on one side, away from the small hole diaphragm, of the collimating lens along the light axis, a second light through hole is formed in the light spot limiting diaphragm, and the axis of the second light through hole is parallel to the light axis.

Wherein the diameter D of the second light through hole satisfies: d is more than or equal to 1.5mm and less than or equal to 3.2mm, and preferably is 2.0 mm.

Wherein, the thickness H of the facula limiting diaphragm along the self axis direction satisfies: h is more than or equal to 5 mm.

Wherein the laser wavelength λ emitted by the laser satisfies: λ is 400nm or more and 1000nm or less, preferably 600nm or more and 700nm or less.

The light source assembly further comprises a depolarizing device, the depolarizing device is arranged between the collimating lens and the aperture stop along the optical axis, and the depolarizing device is used for eliminating the polarization of the laser reflected by the collimating lens so as to avoid the reflected laser and the laser from generating a self-coherent effect.

In a second aspect, the invention provides a specific protein analysis system, which includes a light source assembly, a colorimetric pool and a signal acquisition device, wherein the colorimetric pool is disposed between the light source assembly and the signal acquisition device, the light source assembly emits laser toward the colorimetric pool along a first light path, the laser is received by the signal acquisition device after passing through the colorimetric pool, the colorimetric pool includes a light incident surface facing the light source assembly, and the light incident surface forms a non-perpendicular second included angle with respect to the first light path, so as to avoid interference of the laser reflected by the light incident surface with the laser of the first light path.

Wherein, the second included angle β of the light incident surface of the colorimetric pool relative to the first light path satisfies: beta is more than or equal to 75 degrees and less than or equal to 87 degrees, preferably 83 degrees.

And the light incident surface of the colorimetric pool is provided with an antireflection film for reducing the light reflectivity.

Wherein the specific protein analysis system is a transmission specific protein analysis system.

The light source assembly may be the light source assembly described above.

In a third aspect, the specific protein analysis system provided by the present invention includes a light source assembly, a colorimetric pool, and a signal acquisition device, where the colorimetric pool is disposed between the light source assembly and the signal acquisition device, the signal acquisition device includes a light-sensing surface disposed toward the colorimetric pool, the light source assembly emits laser toward the colorimetric pool, the laser is emitted along a first light path after passing through the colorimetric pool and is received by the light-sensing surface, and the light-sensing surface forms a non-perpendicular third included angle with respect to the first light path, so as to avoid interference of the laser reflected by the light-sensing surface with the laser of the first light path.

Wherein the third included angle γ of the photosensitive surface with respect to the first optical path satisfies: gamma is between 78 DEG and 89 DEG, preferably 87 deg.

The specific protein analysis system further comprises a depolarizing device, the depolarizing device is arranged between the signal acquisition device and the colorimetric pool along the first light path, and the depolarizing device is used for eliminating the polarization of laser reflected by the signal acquisition device so as to avoid the reflected laser and the light source assembly from generating a self-coherent effect.

In a first aspect of the invention, a light source assembly for use in a particular protein analysis system includes a laser, an aperture stop, and a collimating lens. The laser emitted by the laser can penetrate through the small-hole diaphragm and is projected onto the collimating lens, and the laser is emitted out after being collimated by the collimating lens, so that the light emitted by the light source component to the rear end is parallel light suitable for detection. And the axis of the first light through hole of the small-hole diaphragm is not coincident with the optical axis of the collimating lens, so that reflected light formed by the collimating lens can be controlled, the reflected light is prevented from reflecting back towards the laser, the light emitted by the laser is prevented from being influenced by an external field interference effect, the power of the light output by the laser and the stability of the output wavelength are improved, and the quality of a detection light source is improved.

In the second aspect and the third aspect of the present invention, the colorimetric pool and the signal acquisition device are respectively arranged relative to the laser light path, so as to avoid the influence of the light reflected by the colorimetric pool or the signal acquisition device on the detection light source. And the arrangement of the light source component in the first aspect of the invention is combined, so that the precision of the specific protein analysis system is improved, and the reliability and effectiveness of detection data are ensured.

Drawings

FIG. 1 is a schematic view of a light source assembly according to the present invention;

FIG. 2 is a schematic view of another embodiment of a light source module according to the present invention;

FIG. 3 is a schematic view of another embodiment of a light source module according to the present invention;

FIG. 4 is a schematic view of another embodiment of a light source module according to the present invention;

FIG. 5 is a schematic view of another embodiment of a light source module according to the present invention;

FIG. 6 is a schematic view of another embodiment of a light source module according to the present invention;

FIG. 7 is a schematic view of another embodiment of a light source module according to the present invention;

FIG. 8 is a schematic diagram of a specific protein analysis system of the present invention;

FIG. 9 is a schematic view of another embodiment of a specific protein analysis system of the present invention;

FIG. 10 is a schematic representation of another specific protein analysis system of the present invention;

FIG. 11 is a schematic representation of another specific protein analysis system of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" as used herein includes both direct and indirect connections (couplings), unless otherwise specified. In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

Please refer to fig. 1 illustrating a light source module 100 according to the present invention. The light source assembly 100 is applied to a specific protein analysis system 200 for providing a laser light source for detection of detection items such as C-reactive protein. The light source assembly 100 of the present invention includes a laser 10, an aperture stop 20 and a collimating lens 30, wherein the aperture stop 20 is disposed between the laser 10 and the collimating lens 30. A first light passing hole 21 is formed in the aperture stop 20, the first light passing hole 21 is used for allowing a part of the laser light emitted by the laser 10 to pass through and project onto the collimating lens 30, and the rest of the laser light cannot irradiate onto the collimating lens 30 due to the obstruction of the aperture stop 20. The arrangement of the first light passing hole 21 can keep the divergence angle of the laser light projected onto the collimating lens 30 within a certain range, so that the situation that a large phase difference exists inside a detection light source emitted by the light source assembly 100 and the quality of the light source to be detected is influenced is avoided. The laser beam passing through the first light passing hole 21 forms a parallel beam after being collimated by the collimating lens 30 and then is emitted to the rear end, so that the detection work of related items can be realized.

The first light passing hole 21 is a through hole structure extending linearly along the axis 211. It is understood that the axis 211 of the first light passing hole 21 is preferably parallel to the arrangement direction between the laser 10 and the collimating lens 30, so as to reduce the refraction of the laser light during passing through the first light passing hole 21. The collimating lens 30 has an optical axis 31, and the parallel light beams collimated by the collimating lens 30 are emitted toward the rear end in a direction generally parallel to the optical axis 31. In the light source assembly 100 of the present invention, the axis 211 of the first light passing hole 21 is not aligned with the optical axis 31 of the collimating lens 30, so as to prevent the laser light reflected by the collimating lens 30 after being projected onto the collimating lens 30 from being reflected back into the laser 10, which may interfere with the laser 10.

Please refer to fig. 2 illustrating a light source assembly 100 according to the present invention. Specifically, the collimating lens 30 includes an incident surface 301 close to the aperture stop 20, and the incident surface 301 may be a plane or an arbitrary curved surface. After the laser light is projected onto the light incident surface 301, a part of the laser light is reflected by the light incident surface 301. Referring to FIG. 2, when the light incident surface 301 is planar, the light incident surface 301 is disposed generally perpendicular to the light axis 31. In this case, it is necessary to set the axis 211 of the first light passing hole 21 and the optical axis 31 of the collimating lens 30 to intersect, and the intersecting axis 211 forms a first included angle α with the optical axis 31. An included angle between the reflected light ray L2 reflected by the light incident surface 301 and the incident light ray L1 projected to the light incident surface 301 is 2 α. In the illustration of fig. 2, an incident light L1 emitted from the laser 10 is projected onto the light incident surface 301 along the horizontal direction, and an included angle between the reflected light L2 and the horizontal direction is 2 α. Further, in order to avoid the reflected light L2 from entering the laser 10, it is necessary to control the reflected light L2 reflected by the light incident surface 301 not to pass through the first through hole 21. That is, by setting the first included angle α, the reflected light L2 is not incident on the first light passing hole 21, or the reflected light L2 incident on the first light passing hole 21 can only be irradiated onto the inner wall 212 of the first light passing hole 21, and cannot be reflected back to the laser 10 through the first light passing hole 21. In the illustration of fig. 2, the reflected light L2 is most far reflected only to the side of the inner wall 212 of the first light passing hole 21 close to the laser 10.

It will be appreciated that the first light passing aperture 21 serves as the only light path between the laser 10 and the collimating lens 30, and may also cause the laser light reflected by the collimating lens 30 to be reflected back onto the laser 10 while allowing the light emitted by the laser 10 to pass through and impinge on the collimating lens 30. In the embodiment of fig. 2, since the light incident surface 301 of the collimating lens 30 is a plane, the misalignment between the axis 211 of the first light passing hole 21 and the optical axis 31 of the collimating lens 30 is set to form a first included angle α by intersecting the axis 211 and the optical axis 31, and the angle of the first included angle α is further controlled, so that the reflected light L2 can be prevented from passing through the first light passing hole 21 and being reflected back to the laser 10. The value of the first included angle α is correlated with the distance between the laser 10 and the collimating lens 30, the distance between the aperture stop 20 and the laser 10, the length of the first light passing hole 21, and other dimensions. Theoretically, if the distance between the laser 10 and the collimating lens 30 is close enough, the first included angle α required to be set is large, and the value can reach 25 ° or more; if the distance between the laser 10 and the collimating lens 30 is relatively long, the first included angle α required to be set is relatively small, and may be 3 ° or less. In the embodiment of the present invention, the first included angle α may be defined to satisfy: alpha is more than or equal to 3 degrees and less than or equal to 25 degrees, and the light-emitting requirement of the light source assembly 100 under most use scenes can be met.

It should be noted that, for the embodiment in which the axis 211 forms the first included angle α with the optical axis 31, that is, the collimating lens 30 is disposed obliquely with respect to the axis 211 of the first light passing hole 21, the same applies to the case where the light incident surface 301 is a curved surface. Referring to fig. 3, when the light incident surface 301 is a convex surface, the included angle between the reflected light L2 and the axis 211 is smaller than 2 α, but the effect of controlling the reflected light L2 not to be reflected back to the laser 10 can still be achieved.

On the other hand, the collimator lens 30 further includes a light exit surface 302 opposite to the light entrance surface 301. When the light exits the collimating lens 30 through the light exit surface 302, the light is also reflected by the light exit surface 302. In order to prevent the light reflected by the light-emitting surface 302 from reflecting back to the laser 10, the curvature of the light-emitting surface 302 needs to be set by the collimating lens 30 of the present invention. Generally, since the optical axis 31 of the collimating lens 30 is disposed obliquely with respect to the axis 211 of the first through hole 21, the light reflected by the light-incident surface 301 is prevented from being reflected back into the laser 10, and the light reflected by the light-emitting surface 302 is also prevented from being reflected back into the laser 10.

The light source assembly 100 of the present invention is disposed at an angle between the optical axis 31 of the collimating lens 30 and the axis 211 of the first through hole 21, and the inclination direction of the collimating lens 30 relative to the first through hole 21 is not limited. Collimating lens 30 may be tilted in a vertical direction such that optical axis 31 forms an angle with axis 211, and collimating lens 30 may also be tilted in a horizontal direction such that optical axis 31 forms an angle with axis 211. It will be appreciated that the collimating lens 30 can also be tilted in any direction to form an angle between the optical axis 31 and the axis 211, without affecting the practice of the inventive arrangements.

Referring to fig. 4, in an embodiment corresponding to the embodiment in which the light incident surface 301 is a curved surface, the collimating lens 30 may be disposed off-axis (off-axis) to prevent the reflected light L2 from being reflected back to the laser 10. Specifically, the optical axis 31 of the collimating lens 30 is disposed parallel to the axis 211 of the first light passing hole 21, and a specific distance d is formed between the optical axis 31 and the axis 211. That is, the optical axis 31 of the collimator lens 30 is disposed off-axis (off-axis) with respect to the axis 211 of the first light passing hole 21 by a certain distance d.

As can be seen from fig. 4, since the light incident surface 301 of the collimating lens 30 is a curved surface, after the light axis 31 is disposed off-axis (off-axis) with respect to the axis 211 of the first light passing hole 21, the incident light L1 transmitted from the first light passing hole 21 directly irradiates the curved light incident surface 301, so that the reflected light L2 also forms a specific included angle with respect to the axis 211. When the curvature of the light incident surface 301 is constant, the reflected light ray L2 can be controlled not to be reflected back into the laser 10 by defining the specific distance d.

Similarly, if the curvature of the light incident surface 301 is large, the reflection angle formed by the incident light L1 is correspondingly enlarged, and the value of the specific distance d may be relatively small, that is, the specific distance d from the axis 211 of the first light passing hole 21 to the optical axis 31 may be small; on the contrary, if the curvature of the light incident surface 301 is small, the reflection angle formed by the incident light L1 is correspondingly small, and the value of the specific distance d may be relatively large. Meanwhile, in combination with the size of the diameter D1 of the collimating lens 30, in order to prevent most of incident light L1 from irradiating the edge of the collimating lens 30 to affect the collimating effect, the value of the specific distance D can be limited to satisfy: d is not less than D1/4 and not more than D1/100, so that the condition that the reflected light ray L2 in most use scenes retroreflects to the laser 10 can be avoided on the premise that the collimation effect of the collimating lens 30 on the incident light ray L1 is met.

Therefore, in the embodiment of the present invention, the optical axis 31 of the collimating lens 30 is disposed obliquely or off-axis (off-axis) with respect to the axis 211 of the first light passing hole 21, so that the reflected light L2 is not directly reflected back to the laser 10, and the defect that the power and wavelength of the light output by the laser 10 are unstable due to triggering the external field interference effect of the laser 10 when the light reflected by the collimating lens 30 is reflected back to the laser 10 is effectively avoided. By any arrangement of the above manners, the precision and quality of the laser emitted by the light source assembly 100 of the present invention can be ensured, so as to meet the requirements of high sensitivity and high precision detection.

It should be noted that the non-coincidence between the optical axis 31 and the axis 211 may also be provided in a tilted and off-axis (off-axis) configuration. That is, the light axis 31 is inclined relative to the axis 211 to form the first included angle α, and the light axis 31 is offset relative to the axis 211 by a specific distance d, so that the reflected light L2 reflected by the light incident surface 301 is ensured not to be reflected back into the laser 10 by the cooperation of the two. The two structures can further reduce the volume of the light source assembly 100 of the present invention, so that the collimating lens 30 provides a more accurate detection light source to the rear end under the premise of a smaller inclination angle or offset distance relative to the first light passing hole 21.

In the case of the aperture stop 20, when the laser light emitted by the laser 10 is projected onto the aperture stop 20 or the laser light reflected by the collimating lens 30 is reflected back onto the aperture stop 20, in order to avoid the aperture stop 20 from forming secondary reflection to the light to further interfere with the detection light source, it is preferable to provide the aperture stop 20 with a light-absorbing material (see fig. 2), or to coat or plate a light-absorbing film layer 22 on the surface of the aperture stop 20 (see fig. 3). It is understood that the aperture stop 20 made of light absorbing material, or the aperture stop 20 provided with the light absorbing film layer 22, can absorb the light projected or reflected onto the aperture stop 20, so as to avoid the secondary reflection phenomenon of the light. Referring to fig. 2, for the aperture stop 20 made of a light absorbing material, the inner wall 212 of the first light passing hole 21 is also made of a light absorbing material, and when the laser is projected or reflected to the inner wall 212 of the first light passing hole 21, the light absorbing material can prevent the laser from being repeatedly refracted in the first light passing hole 21. For the embodiment of fig. 3, when the light absorbing film layer 22 is disposed, the light absorbing film layer 22 is preferably disposed on the inner wall 212 of the first light passing hole 21, so as to avoid repeated refraction of the laser in the first light passing hole 21.

Referring to fig. 4, an antireflection film 32 may be further disposed at the light incident surface 301 of the collimating lens 30. The antireflection film 32 is used to reduce the reflectivity of the incident surface 301 to the incident light L1, and further reduce the influence of the reflected light L2 on the laser 10. Preferably, the antireflection film 32 controls the reflectivity at the light incident surface 301 to be less than 2.5 ‰, so that even if part of the reflected light L2 is reflected back to the laser 10 through different paths, the interference of the reflected light L2 to the laser 10 can be reduced.

In one embodiment, the antireflection film 32 is also disposed at the light-emitting surface 302 for reducing the reflectivity of the light-emitting surface 302 to the incident light. In this case, the antireflection films 32 are disposed on two opposite sides of the outer surface of the collimating lens 30, so that the influence of the collimating lens 30 on the light emitted by the laser 10 is further eliminated. It can be understood that the reflectivity of the antireflection film 32 at the light emitting surface 302 is also controlled to be less than 2.5 ‰.

Referring to fig. 5, an isolator 80 aligned with the first light passing hole 21 is further disposed between the aperture stop 20 and the collimating lens 30 along the axis 211 of the first light passing hole 21. The isolator 80 is disposed toward the collimating lens 30 and is configured to block the laser light reflected by the light incident surface 301 of the collimating lens 30. The isolator 80 is a functional device commonly used in the optical field, which allows light to pass through in one direction and blocks light reflected back in the opposite direction. Therefore, when the isolator 80 is disposed along the axis 211 of the first light passing hole 21 toward the collimating lens 30, the laser light propagating along the first light passing hole 21 can be projected onto the light incident surface 301, and the laser light reflected by the light incident surface 301 can be blocked.

Referring to the embodiment of fig. 6, the light source assembly 100 further includes a spot limiting diaphragm 40. The light spot limiting diaphragm 40 is arranged on a side of the collimating lens 30 away from the aperture stop 20, i.e. the collimating lens 30 is arranged between the aperture stop 20 and the light spot limiting diaphragm 40. And because the parallel light beams collimated by the collimating lens 30 are emitted along the optical axis 31 thereof, the flare limiting diaphragm 40 is preferably disposed along the optical axis 31. The spot limiting diaphragm 40 is used to control the spot size formed by the parallel beam. Specifically, the spot limiting diaphragm 40 is provided with a second light passing hole 41, and an axis 411 of the second light passing hole 41 is parallel to the optical axis 31 of the collimator lens 30. Preferably, for the embodiment in which the optical axis 31 of the collimating lens 30 is disposed obliquely with respect to the axis 211 of the first light passing hole 21, the axis 411 of the second light passing hole 41 and the optical axis 31 of the collimating lens 30 may also be disposed coincidentally (see fig. 7), so that the parallel light beams emitted from the collimating lens 30 can pass through the second light passing hole 41 in parallel. Since the spot limiting diaphragm 40 has a certain thickness H along the axis 411 of the second light passing hole 41 and a certain diameter D matching the second light passing hole 41, the size of the spot formed by the parallel light beam can be controlled by setting the length (i.e., the thickness H of the spot limiting diaphragm 40) and the diameter D of the second light passing hole 41. I.e., the spot size of the detection light source emitted by the light source assembly 100.

In the embodiment of the present invention, the diameter D of the second light passing hole 41 satisfies the condition: d is 1.5mm ≦ D ≦ 3.2mm, and D is preferably 2.0 mm. Accordingly, the thickness H of the limiting-spot limiting diaphragm 40 in the direction of the own axis 41 satisfies the condition: h is more than or equal to 5 mm. The arrangement of the spot limiting diaphragm 40 along the thickness H in the direction of the axis 41 can also eliminate the aperture diffraction effect formed by the parallel light beams after passing through the second light passing hole 41. If the length of the second light through hole 41, that is, the thickness H of the light spot limiting diaphragm 40 is too small and close to the wavelength of the parallel light beam, the parallel light beam is prone to generate a diffraction effect in the process of passing through the second light through hole 41, so that the detection light source emitted by the light source assembly 100 forms a light spot shape which is annularly diffused and propagates backwards with the axis 411 of the second light through hole 41 as the center. Therefore, the thickness H of the spot limiting diaphragm 40 is limited to be not less than 5mm at least, so that the length of the second light passing hole 41 is obviously different from the wavelength of the laser light emitted by the laser 10, and the aperture diffraction effect is avoided.

For one embodiment, for the laser 10 of the light source assembly 100 of the present invention, the laser wavelength λ emitted by the laser needs to satisfy the following condition: λ is 400nm or more and 1000nm or less, preferably 600nm or more and 700nm or less. Laser light in this wavelength range is suitable for turbidimetric detection of the specific protein analysis system 200.

Referring to fig. 7, the light source assembly 100 further includes a depolarizing device 50. The depolarizing means 50 may be arranged between the collimator lens 30 and the aperture stop 20 in the direction of the optical axis 31 of the collimator lens 30. The depolarizing means 50 is aligned with the collimating lens 30 for depolarizing the laser light reflected by the collimating lens 30, i.e. for depolarizing the polarization state of the reflected light L2, so as to avoid that the laser light reflected by the collimating lens 30 forms an auto-coherence effect with the laser light emitted by the laser 10.

As mentioned above, the collimating lens 30 has an optical axis 31, and the parallel light beams collimated by the collimating lens 30 are emitted toward the rear end in a direction generally parallel to the optical axis 31 (as shown in fig. 6 and 7). Of course, there are also embodiments as shown in fig. 5, which can also make the parallel light beam exit to the rear end in a direction oblique to the optical axis 31 by providing a curved surface to the collimator lens 30. The inclined angle is the same as the angle between the axis 211 of the first light passing hole 21 and the optical axis 31, so that the light rays are emitted toward the rear end in a direction parallel to the axis 211 of the first light passing hole 21 after passing through the obliquely arranged collimating lens 30. Such a light path design can arrange the light source module 100 and the rear device on the same line, which is beneficial to reduce the volume of the whole specific protein analysis system.

Please refer to fig. 8 showing a specific protein analysis system 200 according to the present invention. The specific protein analysis system 200 includes a light source module 100, a cuvette 60 and a signal acquisition device 70. Wherein colorimetric pool 60 sets up between light source subassembly 100 and signal acquisition device 70, and light source subassembly 100 sends the laser as detecting light source towards colorimetric pool 60 along first light path 101, and the laser is received by signal acquisition device 70 behind colorimetric pool 60. The signal acquisition device 70 can measure and calculate the absorbance and the scattering degree of the sample in the colorimetric pool 60 to the laser light by comparing the brightness and the intensity of the received laser with the brightness and the intensity of the laser emitted by the light source assembly 100, and further calculate the antigen content in the sample, so as to achieve the purpose of carrying out turbidimetric detection on the sample in the colorimetric pool 60.

In the specific protein analysis system 200 of the present invention, the cuvette 60 also includes an incident surface, and the incident surface of the cuvette 60 includes a first incident surface 61 facing the light source assembly 100 and a second incident surface 62 opposite to the first incident surface 61. The first light incident surface 61 is a surface of the outer wall of the color cell 60 close to the light source assembly 100 along the first light path 101, and the second light incident surface 62 is a surface of the inner wall of the color cell far away from the light source assembly 100 along the first light path 101. The laser enters the cuvette 60 from the first light incident surface 61 along the first light path 101, is absorbed and scattered by the sample in the cuvette 60, then enters the second light incident surface 62, and finally exits from the cuvette 60. The cuvette 60 serves as a container for testing, and when the laser light is projected to the first light incident surface 61 along the first light path 101, the first light incident surface 61 reflects the laser light. It is understood that the laser light reflected by the first light incident surface 61 may interfere with the laser light emitted by the light source assembly 100 if the laser light is reflected back into the light source assembly 100. For this reason, in the specific protein analysis system 200 of the present invention, the angle between the first light incident surface 61 and the first light path 101 is also set accordingly, so as to prevent the laser light reflected by the first light incident surface 61 from being reflected back to the light source assembly 100 along the first light path 101.

Generally, the light source assembly 100 projects the laser light into the color cell 60 in a normal incidence manner, i.e., the first light path 101 is vertically disposed between the first light incident surface 61. However, the first light incident surface 61 and the first light path 101 perpendicular to each other cause the laser light reflected by the first light incident surface 61 to be reflected back to the light source assembly 100 along the first light path 101. Therefore, in the specific protein analysis system 200 of the present invention, it is necessary to set the first light incident surface 61 and the first light path 101 to form a non-perpendicular second included angle β, so as to prevent the laser light reflected by the first light incident surface 61 from being reflected back to the light source assembly 100 along the first light path 101 to interfere with the laser light emitted from the light source assembly 100.

Similar to the structure of the first included angle α, in the schematic diagram of fig. 8, after the laser light L3 incident from the light source assembly 100 is projected to the first light incident surface 61 along the first light path 101, because the first light incident surface 61 forms a second included angle β which is not perpendicular to the first light path 101, the incident angle of the incident laser light L3 with respect to the first light incident surface 61 is "90 ° - β". Accordingly, the angle between the laser light L4 reflected by the first light incident surface 61 and the first light path 101 is "2 × (90 ° - β)". It is understood that the value of the second included angle β in the specific protein analysis system 200 can be derived based on the distance between the light source assembly 100 and the cuvette 60, or as described based on the distance between the light source assembly 100 and the first light incident surface 61. In general, the second included angle β of the first light incident surface 61 with respect to the first light path 101 may satisfy the condition: beta is more than or equal to 75 degrees and less than or equal to 87 degrees, preferably 83 degrees. The situation that most of the laser light L4 reflected by the first light incident surface 61 is reflected back to the light source assembly 100 in the usage scene can be satisfied.

It should be noted that the first optical path 101 is an optical path through which the laser emitted from the light source assembly 100 toward the first incident surface 61 of the cuvette 60 is emitted to the signal acquisition device 70 through the cuvette 60. The non-perpendicular arrangement between the first light incident surface 61 and the first light path 101 may be realized by inclining the cuvette 60, or may be realized by arranging the first light incident surface 61 as an inclined side surface on the cuvette 60. Because the cuvette 60 serves to hold the sample and allow the passage of laser light, the specific shape of the cuvette 60 is not critical in the present embodiment. As long as the first light incident surface 61 of the cuvette 60 disposed in the specific protein analysis system 200 and the first light path 101 form the second included angle β, the situation that the laser L4 reflected by the first light incident surface is reflected back to the light source assembly 100 can be avoided.

The particular protein colorimetric system 200 of the present invention is also not particularly limited with respect to the tilt orientation of the cuvette 60 relative to the light source assembly 100. That is, the cuvette 60 may be tilted with respect to the light source assembly 100 in a vertical direction, or may be tilted with respect to the light source assembly 100 in a horizontal direction or any direction to form an offset of the first light-entering surface 61 and/or the second light-entering surface 62 with respect to the first light path 101, without affecting the implementation of the particular protein colorimetric system 200 of the present invention, and with the same advantages obtained.

On the other hand, the cuvette 60 further has a second light incident surface 62. After entering the cell 60 through the first entrance surface 61, the light rays exit the cell 60 through the second entrance surface 62. In the cuvette 60, the first light incident surface 61 and the second light incident surface 62 are generally parallel to each other. Therefore, by arranging the first light incident surface 61 in an inclined manner, the second light incident surface 62 is also inclined in a synchronous manner, so that the laser light reflected by the second surface is not reflected back to the light source assembly 100. Of course, for the structure of the cuvette 60 in which part of the first light incident surface 61 and the second light incident surface 62 are not arranged in parallel, when the first light incident surface 61 is arranged obliquely, it is also required to ensure that the included angle between the second light incident surface 62 and the first light path 101 is not vertical, so as to prevent the light reflected by the second light incident surface 62 from being reflected back to the light source assembly 100. That is, the light incident surface of the cuvette 60 forms a non-perpendicular second included angle β with respect to the first light path 101, so as to avoid interference of the laser light reflected by the light incident surface with the laser light of the first light path 101.

Referring to fig. 9, the light source assembly 100 of the particular protein analysis system 200 of the present invention may also be the light source assembly 100 described above with reference to any one of the embodiments shown in fig. 1-7. In the embodiment of fig. 9, light source assembly 100 includes laser 10, aperture stop 20, collimating lens 30, and spot limiting stop 40, which are arranged in sequence. To limit the reflection of the laser light L4 reflected by the first light incident surface 61 back to the light source assembly 100 along the first light path 101 to avoid the interference of the reflected light L4 with the light source assembly 100, it can be understood that the laser light L4 reflected by the first light incident surface 61 is limited from reflecting back to the laser 10 to form interference with the laser light emitted by the laser 10. That is, it is necessary to limit the laser light reflected by the first light incident surface 61 to only irradiate the inner wall 212 of the first light passing hole 21, and not to be reflected back to the laser 10 through the first light passing hole 21.

In one embodiment, the antireflection film 32 for reducing the light reflectivity may also be disposed on the first light incident surface 61 and/or the second light incident surface 62. Preferably, the anti-reflection film 32 controls the reflectivity of the first light incident surface 61 and/or the second light incident surface 62 to be less than 2.5 ‰, so that even though part of the laser light L4 reflected by the first light incident surface 61 and/or the second light incident surface 62 is reflected back to the laser 10 through different paths, the interference of the laser light L4 reflected by the first light incident surface 61 and/or the second light incident surface 62 to the laser 10 can be reduced.

In one embodiment, protein specific analysis system 200 is a transmission method protein specific analysis system. That is, the signal acquisition device 70 is disposed at the rear end of the cuvette 60 along the first light path 101, and the signal acquisition device 70 is configured to receive the laser signal emitted by the light source assembly 100 and projected through the cuvette 60, and perform turbidimetry detection on the sample in the cuvette 60.

Another specific protein analysis system 300 of the present invention is shown in FIG. 10. The specific protein analysis system 300 also includes a light source assembly 100, a cuvette 60, and a signal acquisition device 70. Wherein colorimetric pool 60 sets up between light source subassembly 100 and signal acquisition device 70, and light source subassembly 100 sends the laser as detecting light source towards colorimetric pool 60 along first light path 101, and the laser jets out and is received by signal acquisition device 70 along first light path 101 behind colorimetric pool 60. The signal acquisition device 70 includes a photosensitive surface 71 disposed toward the cuvette 60. In this embodiment, it is also necessary to set the light-sensing surface 71 to form a non-perpendicular third angle γ with respect to the first optical path 101, considering that the light-sensing surface 71 may reflect the laser light projected along the first optical path 101.

In the present embodiment, the first optical path 101 is an optical path of the laser light emitted from the cuvette 60 after transmission. In general, in order to facilitate the reception of the laser light by the signal acquisition device 70, the light sensing surface 71 is disposed perpendicular to the first optical path 101 for receiving the optical signal. However, for similar reasons as those described above with respect to the collimating lens 30 and the cuvette 60, the present invention also needs to set the light-sensing surface 71 and the first light path 101 at a non-perpendicular third angle γ, so as to avoid interference of the laser light reflected by the light-sensing surface 71 and reflected back into the light source assembly 100 along the first light path 101.

In the schematic of fig. 10, after the laser light L5 transmitted from the cuvette 60 is projected to the light-sensing surface 71 along the first optical path 101, the incident angle of the transmitted laser light L5 with respect to the light-sensing surface 71 is "90 ° - γ" because the light-sensing surface 71 forms a third non-perpendicular angle γ with the first optical path 101. Accordingly, the angle of the laser light L6 reflected by the light-sensing surface 71 relative to the first optical path 101 is "2 x (90 ° - γ)". The third angle γ of the photosensitive surface 71 with respect to the first optical path 101 is set to satisfy the condition: 78 DEG-gamma-89 DEG, preferably 87 DEG, satisfies that most of the laser light L6 reflected by the light-sensing surface 71 in the scene is reflected back to the light source assembly 100.

It is understood that the light source assembly 100 of a particular protein analysis system 300 may also be configured as the light source assembly 100 of any of the embodiments described above. That is, the laser light L6 reflected by the light-receiving surface 71 is restricted from being reflected back to the light source module 100, and the laser light L6 reflected by the light-receiving surface 71 is also restricted from being reflected back to the laser 10 to interfere with the emission of the laser 10.

Referring to fig. 11, the specific protein analysis system 300 further includes a depolarizing device 50, the depolarizing device 50 is disposed between the signal acquisition device 70 and the cuvette 60 along the first optical path 101, and the depolarizing device 50 is configured to eliminate polarization of the laser light reflected by the photosensitive surface 71 of the signal acquisition device 70, i.e., eliminate a polarization state of the laser light L6 reflected by the photosensitive surface 71 (see fig. 10), so as to avoid a self-coherence effect between the reflected laser light and the light source assembly 100. It can be understood that the depolarizing device 50 can also be disposed between the light source assembly 100 and the cuvette 60, and specifically, the depolarizing device 50 is disposed between the light source assembly 100 and the cuvette 60 along the first light path 101 formed by the transmission of the laser emitted by the light source assembly 100, so as to eliminate the polarization state of the laser reflected by the cuvette 60, so as to prevent the reflected laser and the light source assembly 100 from generating a self-coherent effect.

It should be noted that, for the non-perpendicular arrangement of the light incident surface of the cuvette 60 and the first light path 101 in the specific protein analysis system 200, and the non-perpendicular arrangement of the light sensing surface 71 of the signal acquisition device 70 and the first light path 101 in the specific protein analysis system 300, they can also be applied to the same specific protein analysis system 200/300. As shown in fig. 11, in the specific protein analysis system 200/300, by simultaneously comparing the arrangement of the color cell 60 and the signal acquisition device 70 with respect to the corresponding first light path 101, the interference of the laser light reflected by the first light incident surface 61 with the light source assembly 100 and the interference of the laser light reflected by the light sensing surface 71 with the light source assembly 100 can be avoided. By combining the related arrangement of the light source assembly 100 itself to avoid reflected light interference, the detection result error caused by the related light interference can be eliminated in the turbidimetric detection process of the whole specific protein analysis system 200/300, and the detection accuracy of the specific protein analysis system 200/300 of the present invention is improved.

It is understood that in the embodiment where the isolator 80 is provided for the light source assembly 100, the isolator 80 may also be provided in the specific protein analysis system 200/300 to eliminate the interference of the reflected laser light with the detection light source. In the illustration of fig. 11, the isolator 80 is disposed between the cuvette 60 and the light source assembly 100 along the first light path 101, and is used for blocking the laser light reflected by the first light incident surface 61 from returning to the light source assembly 100. It is understood that the isolator 80 may also be disposed between the cuvette 60 and the signal acquisition device 70 along the first optical path 101 for blocking the laser light reflected by the photosensitive surface 71 from returning to the light source assembly 100.

The above-described embodiments do not limit the scope of the present invention. Any modification, equivalent replacement, and improvement made within the spirit and principle of the above-described embodiments should be included in the protection scope of the technical solution.

Claims (20)

1. The light source assembly is applied to a specific protein analysis system and is characterized by comprising a laser, an aperture diaphragm and a collimating lens, wherein the aperture diaphragm is arranged between the laser and the collimating lens, the aperture diaphragm is provided with a first light through hole which penetrates through the aperture diaphragm, laser emitted by the laser penetrates through the first light through hole and then is collimated and emitted backwards by the collimating lens, and the axis of the first light through hole is not coincident with the optical axis of the collimating lens so as to prevent the laser reflected by the collimating lens from reflecting back to the laser.

2. The light source assembly of claim 1, wherein an axis of the first light passing hole intersects the optical axis of the collimating lens to form a first included angle.

3. The light source assembly according to claim 2, wherein the first included angle α satisfies: alpha is more than or equal to 3 degrees and less than or equal to 25 degrees.

4. The light source assembly of claim 1, wherein the collimating lens includes a light incident surface near the aperture stop, the light incident surface is a curved surface, and an axis of the first light passing hole is parallel to and forms a specific distance with the optical axis of the collimating lens.

5. The light source assembly according to claim 4, wherein the specific distance d satisfies: d is not less than D1/4 and not more than D1/100, wherein D1 is the diameter of the collimating lens.

6. The light source assembly according to any of the claims 1-5, wherein the aperture stop is made of a light absorbing material or is provided with a light absorbing film layer.

7. The light source assembly according to any one of claims 1 to 5, wherein an isolator is arranged between the aperture stop and the collimating lens along the axis of the first light passing hole, and is used for blocking the laser light reflected back by the collimating lens.

8. The light source assembly according to any one of claims 1 to 5, wherein the collimating lens includes an entrance surface adjacent to the aperture stop, and an antireflection film is disposed on the entrance surface for reducing the light reflectance of the entrance surface.

9. The light source module as claimed in claim 8, wherein the collimating lens further comprises a light exit surface opposite the light entrance surface, and the light exit surface is also provided with an antireflection film for reducing the light reflectivity of the light entrance surface.

10. The light source assembly according to any one of claims 1 to 5, further comprising a spot limiting diaphragm for controlling the size of the light spot, the spot limiting diaphragm being disposed along the optical axis on a side of the collimating lens away from the aperture diaphragm, the spot limiting diaphragm being provided with a second light passing hole, and an axis of the second light passing hole being parallel to the optical axis.

11. The light source assembly of claim 9, wherein the diameter D of the second light aperture satisfies: d is more than or equal to 1.5mm and less than or equal to 3.2mm, and preferably is 2.0 mm.

12. The light source assembly according to claim 9, wherein the thickness H of the spot limiting diaphragm in the direction of the axis thereof satisfies: h is more than or equal to 5 mm.

13. The light source assembly according to any one of claims 1 to 5, wherein the laser emits a laser wavelength λ satisfying: λ is 400nm or more and 1000nm or less, preferably 600nm or more and 700nm or less.

14. The utility model provides a specific protein analysis system, its characterized in that, includes light source subassembly, colorimetric pool and signal acquisition device, the colorimetric pool set up in the light source subassembly with between the signal acquisition device, the light source subassembly is followed first light path orientation the colorimetric pool sends laser, laser warp behind the colorimetric pool by signal acquisition device receives, the colorimetric pool includes the orientation the income plain noodles of light source subassembly, the income plain noodles for first light path forms non-vertically second contained angle, in order to avoid by the laser of income plain noodles reflection is right the laser formation of first light path disturbs.

15. The specific protein analysis system of claim 14, wherein the second included angle β between the light incident surface of the cuvette and the first light path satisfies: beta is more than or equal to 75 degrees and less than or equal to 87 degrees, preferably 83 degrees.

16. The specific protein analysis system of claim 14, wherein the light incident surface of the cuvette is provided with an antireflection film for reducing light reflectance.

17. The specific protein assay system of any one of claims 14-16, wherein the specific protein assay system is a transmission specific protein assay system.

18. The specific protein analysis system of claim 14, wherein the light source assembly is the light source assembly of any one of claims 1-13.

19. The utility model provides a specific protein analysis system, its characterized in that, includes light source subassembly, colorimetric pool and signal acquisition device, the colorimetric pool set up in the light source subassembly with between the signal acquisition device, the signal acquisition device is including the orientation the photosurface that the colorimetric pool set up, the light source subassembly orientation the colorimetric pool sends laser, laser warp the colorimetric pool back is jetted out and quilt along first light path the plain noodles is received, the photosurface for first light path forms non-vertically third contained angle, in order to avoid by the laser of photosurface reflection is right the laser of first light path forms the interference.

20. The specific protein analysis system according to claim 19, wherein the third included angle γ of the photosensitive surface with respect to the first optical path satisfies: gamma is between 78 DEG and 89 DEG, preferably 87 deg.

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