CN112913849A - 一种含有异硫氰酸酯类化合物的农药组合物及其在防治植物病害中的用途 - Google Patents
一种含有异硫氰酸酯类化合物的农药组合物及其在防治植物病害中的用途 Download PDFInfo
- Publication number
- CN112913849A CN112913849A CN202110102271.8A CN202110102271A CN112913849A CN 112913849 A CN112913849 A CN 112913849A CN 202110102271 A CN202110102271 A CN 202110102271A CN 112913849 A CN112913849 A CN 112913849A
- Authority
- CN
- China
- Prior art keywords
- essential oil
- active component
- isothiocyanatoethyl
- small molecular
- methoxybenzene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 28
- 201000010099 disease Diseases 0.000 title claims abstract description 17
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 17
- -1 isothiocyanate compounds Chemical class 0.000 title abstract description 14
- 239000000575 pesticide Substances 0.000 title description 7
- 230000002265 prevention Effects 0.000 title description 4
- 239000000341 volatile oil Substances 0.000 claims abstract description 45
- IZJDOKYDEWTZSO-UHFFFAOYSA-N phenethyl isothiocyanate Chemical compound S=C=NCCC1=CC=CC=C1 IZJDOKYDEWTZSO-UHFFFAOYSA-N 0.000 claims abstract description 34
- 241000196324 Embryophyta Species 0.000 claims abstract description 31
- JIZVGEJTLPEYLM-UHFFFAOYSA-N 1-(2-isothiocyanatoethyl)-4-methoxybenzene Chemical compound COC1=CC=C(CCN=C=S)C=C1 JIZVGEJTLPEYLM-UHFFFAOYSA-N 0.000 claims abstract description 26
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 25
- 241000123650 Botrytis cinerea Species 0.000 claims abstract description 12
- 239000003899 bactericide agent Substances 0.000 claims abstract description 12
- 241000813090 Rhizoctonia solani Species 0.000 claims abstract description 11
- 241000221696 Sclerotinia sclerotiorum Species 0.000 claims abstract description 10
- 229920000742 Cotton Polymers 0.000 claims abstract description 8
- 241001330975 Magnaporthe oryzae Species 0.000 claims abstract description 7
- 241000223195 Fusarium graminearum Species 0.000 claims abstract description 6
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims abstract description 6
- 244000223014 Syzygium aromaticum Species 0.000 claims abstract description 6
- 240000002657 Thymus vulgaris Species 0.000 claims abstract description 5
- 235000007303 Thymus vulgaris Nutrition 0.000 claims abstract description 5
- 239000001585 thymus vulgaris Substances 0.000 claims abstract description 5
- 244000223760 Cinnamomum zeylanicum Species 0.000 claims abstract description 4
- 235000017803 cinnamon Nutrition 0.000 claims abstract description 4
- 150000002989 phenols Chemical class 0.000 claims description 21
- 241000223221 Fusarium oxysporum Species 0.000 claims description 3
- 241000223218 Fusarium Species 0.000 abstract description 3
- 241000219146 Gossypium Species 0.000 abstract description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 15
- 230000003385 bacteriostatic effect Effects 0.000 description 12
- 239000001963 growth medium Substances 0.000 description 12
- 230000002195 synergetic effect Effects 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 244000000003 plant pathogen Species 0.000 description 11
- 244000000005 bacterial plant pathogen Species 0.000 description 9
- 229920001817 Agar Polymers 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 244000061456 Solanum tuberosum Species 0.000 description 8
- 235000002595 Solanum tuberosum Nutrition 0.000 description 8
- 239000008272 agar Substances 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- 230000000996 additive effect Effects 0.000 description 5
- 238000013329 compounding Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 235000013628 Lantana involucrata Nutrition 0.000 description 4
- 235000006677 Monarda citriodora ssp. austromontana Nutrition 0.000 description 4
- 240000007673 Origanum vulgare Species 0.000 description 4
- 230000003042 antagnostic effect Effects 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 description 4
- 230000001018 virulence Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 230000000845 anti-microbial effect Effects 0.000 description 3
- 150000002540 isothiocyanates Chemical class 0.000 description 3
- 231100000053 low toxicity Toxicity 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000011885 synergistic combination Substances 0.000 description 3
- SKIHGKNFJKJXPX-UHFFFAOYSA-N 4-isothiocyanato-1-butene Chemical compound C=CCCN=C=S SKIHGKNFJKJXPX-UHFFFAOYSA-N 0.000 description 2
- DBISBKDNOKIADM-UHFFFAOYSA-N 5-isothiocyanato-1-pentene Chemical compound C=CCCCN=C=S DBISBKDNOKIADM-UHFFFAOYSA-N 0.000 description 2
- 240000003291 Armoracia rusticana Species 0.000 description 2
- 235000011330 Armoracia rusticana Nutrition 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 description 2
- 239000005770 Eugenol Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 description 2
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 239000005844 Thymol Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- MDKCFLQDBWCQCV-UHFFFAOYSA-N benzyl isothiocyanate Chemical compound S=C=NCC1=CC=CC=C1 MDKCFLQDBWCQCV-UHFFFAOYSA-N 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- HHTWOMMSBMNRKP-UHFFFAOYSA-N carvacrol Natural products CC(=C)C1=CC=C(C)C(O)=C1 HHTWOMMSBMNRKP-UHFFFAOYSA-N 0.000 description 2
- RECUKUPTGUEGMW-UHFFFAOYSA-N carvacrol Chemical compound CC(C)C1=CC=C(C)C(O)=C1 RECUKUPTGUEGMW-UHFFFAOYSA-N 0.000 description 2
- 235000007746 carvacrol Nutrition 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 229960002217 eugenol Drugs 0.000 description 2
- 125000004383 glucosinolate group Chemical group 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- 229960000790 thymol Drugs 0.000 description 2
- 241000219198 Brassica Species 0.000 description 1
- 235000003351 Brassica cretica Nutrition 0.000 description 1
- 235000003343 Brassica rupestris Nutrition 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- ZOJBYZNEUISWFT-UHFFFAOYSA-N N-allyl isothiocyanate Natural products C=CCN=C=S ZOJBYZNEUISWFT-UHFFFAOYSA-N 0.000 description 1
- QAADZYUXQLUXFX-UHFFFAOYSA-N N-phenylmethylthioformamide Natural products S=CNCC1=CC=CC=C1 QAADZYUXQLUXFX-UHFFFAOYSA-N 0.000 description 1
- 241001529744 Origanum Species 0.000 description 1
- 235000011203 Origanum Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241001138501 Salmonella enterica Species 0.000 description 1
- 241000194021 Streptococcus suis Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 235000016720 allyl isothiocyanate Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000010460 mustard Nutrition 0.000 description 1
- 239000006272 natural pesticide Substances 0.000 description 1
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical compound ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 108010058651 thioglucosidase Proteins 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/46—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=C=S groups
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
技术领域
本发明涉及的杀菌组合物,是以2-苯乙基异硫氰酸酯或1-(2-异硫代氰酰基乙基)-4-甲氧基苯和小分子酚类化合物或植物源精油为有效成分,具体涉及其在防治由油菜菌核病菌、立枯丝核病菌、番茄灰霉病菌、小麦赤霉病菌、棉花枯萎病菌、稻瘟病菌引起的真菌病害上的用途。
背景技术
植物病害是影响农业生产的主要因素之一,根据联合国粮农组织估计,全世界的粮食和棉花生产因病害常年损失在10%以上。目前世界各国对植物病害的防治主要是使用化学药剂,然而,由于化学药剂的长期使用,引起残留、污染、有害生物产生抗药性、伤害非靶标生物甚至破坏生态平衡等一系列严重的问题,因此研究与开发能有效控制植物病原菌的高效、低毒、低残留的新型农药,已成为目前农药创制的首要目标。从植物中寻找抑菌、杀菌活性物质是目前研究开发绿色杀菌剂的重要途径之一。
异硫氰酸酯(isothiocyanates,ITCs)是指分子中含有-N=C=S共轭双键的一类化合物,这类化合物是硫代葡萄糖苷(Glucosinolates,GLS)在植物内源黑芥子酶作用下的水解产物,是辣根、芥末、山葵等十字花科植物中重要的化学组分,拥有辛辣刺激性的味道。它具有高度生物学活性,研究表明,ITCs能够有效的防止饮食中的多种致癌物,包括多环芳烃、杂环胺和亚硝胺所引起的DNA损伤和癌症。此外,异硫氰酸酯类化合物还具有优秀的抑菌活性,其烯丙基异硫氰酸酯能够在一定程度上抑制酵母菌、细菌和霉菌的生长。此外3-丁烯基异硫氰酸酯、4-戊烯基异硫氰酸酯、2-苯乙基异硫氰酸酯以及苄基异硫氰酸酯对四种革兰氏阳性菌2革兰氏阴性菌有较好的抑菌效果。
植物精油是从植物根、茎、叶、果实等部分提取的,具有强烈芳香味的油状混合物,主要成分以挥发性芳香物质(85-99%)为主,包括萜烯、萜类化合物,酚类等成分。本组合中使用的四种植物精油:牛至、百里香、肉桂、丁香精油关于抑菌活性方面也有相应报道。并且,丁香酚为丁香精油中的主要成分,百里酚是百里香精油中的主要成分,香芹酚在牛至精油中的占比较大。天然酚类化合物是低分子量天然存在的有机化合物,其含有一个或多个酚羟基。天然小分子酚因其结构上含有酚羟基而具有抗氧化、抗肿瘤、抑菌、抗炎等多种生物活性,对人类健康发挥着重要作用。目前小分子酚类化合物在抑菌方面的研究已成为热点之一。据吕世明等的研究表明丁香酚对金黄色葡萄球菌、猪霍乱沙门氏菌及猪链球菌等12种常见的细菌均有较好的抑菌效果;据报道,百里酚对于霉菌、细菌以及临床酚类的真菌均有较好的抑菌效果;谢强等食用香芹酚对革兰氏阴性菌大肠杆菌和革兰氏阳性菌金黄色葡萄球菌通过破坏其细胞膜而产生良好的抑菌活性。值得注意的是,植物精油与小分子酚类化合物尽管具有良好的抑菌活性,但其毒性却很低,具备天然源农药的优点,故其作为防治重要农作物病害的杀菌剂有良好的开发前景。
本发明通过大量活性测试发现,异硫氰酸酯类化合物与小分子酚类化合物以及植物精油的组合应用,在防治立枯丝核菌、油菜菌核、番茄灰霉、小麦赤霉、稻瘟和棉花枯萎等真菌引起的植物病害具有明显的协同增效作用。降低了主剂异硫氰酸酯类化合物的用量,从而在减少毒性的同时提高了防治效果。目前,尚无2-苯乙基异硫氰酸酯或1-(2-异硫代氰酰基乙基)-4-甲氧基苯和小分子酚类化合物以及植物源精油组合杀菌方面的报道。
发明内容
本发明的目的是提供一种含有2-苯乙基异硫氰酸酯或1-(2-异硫代氰酰基乙基)-4-甲氧基苯和小分子酚类化合物以及植物源精油的杀菌剂组合物,用于防治由油菜菌核病菌、立枯丝核病菌、小麦赤霉病菌、番茄灰霉病菌、稻瘟病菌、棉花枯萎病菌引起的病害中的应用。
为实现上述目的,本发明提供了如下技术方法:
一种含有2-苯乙基异硫氰酸酯或1-(2-异硫代氰酰基乙基)-4-甲氧基苯和小分子酚类化合物以及植物源精油的杀菌剂组合物,包括活性组分A与活性组分B,所述活性组分A为2-苯乙基异硫氰酸酯或1-(2-异硫代氰酰基乙基)-4-甲氧基苯,所述活性组分B为小分子酚类化合物或植物源精油。活性组分A与活性组分B的重量比为1:1,1:5。
组合物作为新型农用杀菌剂,具有以下优势:
1)小分子酚类化合物和精油是天然活性成分。其作为杀菌剂具有低毒、低残留、环境相容、不易产生抗性等特点,能够开发为用于生产绿色、无污染农产品的植物源农药。
2)异硫氰酸酯类化合物杀菌活性高。在一定配比范围内,杀菌组合物表现出明显的增效作用,组合物的防治效果与单剂相比有了明显提高。
3)本发明提供的杀菌组合物的有效成分作用机制不相同,有利于病害的综合防治。
为了更好地理解本发明,以下通过具体实施方式,对本发明的上述内容做进一步的详细说明。但不应将此理解为对本发明的限制。
具体实施方式
实施例1:异硫氰酸酯类化合物与小分子酚类化合物的杀菌组合物,包括活性组分A与活性组分B(如结构所示),所述活性组分A为2-苯乙基异硫氰酸酯(Y-1)或1-(2-异硫代氰酰基乙基)-4-甲氧基苯(Y-2),纯度>98%;所述活性组分B1为小分子酚类化合物,纯度>98%。
实施例2:2-苯乙基异硫氰酸酯和活性组分对六种植物病原菌室内抑菌活性测定及结果
本实验中所用的植物病原菌为实验室4℃保存的菌种,采用的培养基为马铃薯琼脂葡萄糖培养基(简称PDA)。PDA培养基配方:马铃薯(去皮)200g,葡萄糖20g,琼脂15g,蒸馏水1000mL,自然pH。
杀菌剂的混配增效作用依照Wadley(1967)公式计算不同药剂混合后的协同作用系数:
式中a,b代表2-苯乙基异硫氰酸酯(A),小分子酚类化合物(B1)两种药剂在混合物中所占比率EC50(A),EC50(B)分别表示实际观测EC50值,EC50(Exp)表示A,B两种药剂按a:b混合后的理论EC50值,EC50(Obs)为A,B两种药剂按a:b混合后的实际观测EC50值。SR>1.5为增效;SR<0.5为拮抗;SR介于0.5和1.5之间表示相加作用。
活性组分A与活性组分B单剂的室内抗菌活性
活性如表1所示
表1.活性组分A(Y-1)与活性组分B1的室内抗菌活性
EC50 | Y-1 | P1 | P2 | P3 |
立枯丝核 | 10.36 | 60.452 | 34.405 | 29.74 |
油菜菌核 | 6.24 | 118.125 | 63.96 | 18.83 |
番茄灰霉 | 24.5 | 86.544 | 33.41 | 14.75 |
小麦赤霉 | 3.95 | 87.816 | 45.246 | 29.703 |
稻瘟病 | 12.91 | 91.901 | 41.244 | 44.455 |
棉花枯萎 | 42.37 | 123.258 | 47.84 | 29.06 |
2-苯乙基异硫氰酸酯和活性组分B1复配对6种植物病原菌的毒力增效作用如表2所示
表2.Y-1和活性组分B1复配对6种植物病原菌的毒力增效作用(w/w=1:1)
对1:1(w/w)出现协同的组合进行1:5(w/w)扩比例测定
表3.Y-1和活性组分B1复配对6种植物病原菌的毒力增效作用(w/w=1:5)
分析表2&表3结果可以看出,2-苯乙基异硫氰酸酯(Y-1)与P3复配效果较好在1:1比例时4种病原菌均出现了增效,并在1:5比例时展示出了相加效果。而2-苯乙基异硫氰酸酯和P1在质量比1:5下,SR高达4.21,展示出了明显的增效效果。
实施例3:1-(2-异硫代氰酰基乙基)-4-甲氧基苯和活性组分B1对六种植物病原菌室内抑菌活性测定及结果
本实验中所用的植物病原菌为实验室4℃保存的菌种,采用的培养基为马铃薯琼脂葡萄糖培养基(简称PDA)。PDA培养基配方:马铃薯(去皮)200g,葡萄糖20g,琼脂15g,蒸馏水1000mL,自然pH。
杀菌剂的混配增效作用依照Wadley(1967)公式计算不同药剂混合后的协同作用系数:
式中a,b代表1-(2-异硫代氰酰基乙基)-4-甲氧基苯(A),小分子酚类化合物(B1)两种药剂在混合物中所占比率EC50(A),EC50(B)分别表示实际观测EC50值,EC50(Exp)表示A,B两种药剂按a:b混合后的理论EC50值,EC50(Obs)为A,B两种药剂按a:b混合后的实际观测EC50值。SR>1.5为增效;SR<0.5为拮抗;SR介于0.5和1.5之间表示相加作用。
活性组分A与活性组分B1单剂的室内抗菌活性
活性如表4所示
表4.活性组分A(Y-2)与活性组分B1的室内抗菌活性
EC50 | Y-2 | P1 | P2 | P3 |
立枯丝核 | 41.9 | 60.452 | 34.405 | 29.74 |
油菜菌核 | 32.14 | 118.125 | 63.96 | 18.83 |
番茄灰霉 | 237.17 | 86.544 | 33.41 | 14.75 |
小麦赤霉 | 5.27 | 87.816 | 45.246 | 29.703 |
稻瘟病 | 192.99 | 91.901 | 41.244 | 44.455 |
棉花枯萎 | 148.02 | 123.258 | 47.84 | 29.06 |
Y-2和活性组分B1复配对6种植物病原菌的毒力增效作用如表5所示
表5.Y-2和活性组分B1复配对6种植物病原菌的毒力增效作用(w/w=1:1)
对1:1出现协同的组合进行1:5扩比例测
表6.Y-2和活性组分B1复配对6种植物病原菌的毒力增效作用(w/w=1:5)
分析表5&表6结果可以看出,1-(2-异硫代氰酰基乙基)-4-甲氧基苯(Y-2)与三种小分子酚类化合物在重量比为1:1时,对立枯丝核菌,油菜菌核菌,番茄灰霉菌菌展现出明显的协同增效效果,尤其是重量比为1:5时,Y-2与P2对油菜菌核菌的效果显著,SR值达到4.02。
实施例4:异硫氰酸酯类化合物与植物精油的杀菌组合物,包括活性组分A与活性组分B2,所述活性组分A为2-苯乙基异硫氰酸酯(Y-1)或1-(2-异硫代氰酰基乙基)-4-甲氧基苯(Y-2),纯度>98%;所述活性组分B2为四种植物精油牛至精油,丁香精油,百里香精油和肉桂精油,纯度>98%。
实施例5:2-苯乙基异硫氰酸酯和活性组分B2对六种植物病原菌室内抑菌活性测定及结果
本实验中所用的植物病原菌为实验室4℃保存的菌种,采用的培养基为马铃薯琼脂葡萄糖培养基(简称PDA)。PDA培养基配方:马铃薯(去皮)200g,葡萄糖20g,琼脂15g,蒸馏水1000mL,自然pH。
杀菌剂的混配增效作用依照Wadley(1967)公式计算不同药剂混合后的协同作用系数:
式中a,b代表2-苯乙基异硫氰酸酯(A),植物精油(B2)两种药剂在混合物中所占比率EC50(A),EC50(B)分别表示实际观测EC50值,EC50(Exp)表示A,B两种药剂按a:b混合后的理论EC50值,EC50(Obs)为A,B两种药剂按a:b混合后的实际观测EC50值。SR>1.5为增效;SR<0.5为拮抗;SR介于0.5和1.5之间表示相加作用。
活性组分A与活性组分B2单剂的室内抗菌活性
活性如表7所示
表7.活性组分A(Y-1)与活性组分B2的室内抗菌活性
Y-1和活性组分B2复配对6种植物病原菌的毒力增效作用如表8所示
表8.Y-1和活性组分B2复配对6种植物病原菌的毒力增效作用(w/w=1:1)
对1:1出现协同的组合进行1:5扩比例测定
表9.Y-1和活性组分B2复配对6种植物病原菌的毒力增效作用(w/w=1:5)
表9结果可以看出,2-苯乙基异硫氰酸酯(Y-1)与牛至精油的组合效果较好,尤其是在重量比为1:5时,对棉花枯萎病菌的效果最好,SR值为4.64。
实施例6:1-(2-异硫代氰酰基乙基)-4-甲氧基苯和活性组分B2对六种植物病原菌室内抑菌活性测定及结果
本实验中所用的植物病原菌为实验室4℃保存的菌种,采用的培养基为马铃薯琼脂葡萄糖培养基(简称PDA)。PDA培养基配方:马铃薯(去皮)200g,葡萄糖20g,琼脂15g,蒸馏水1000mL,自然pH。
杀菌剂的混配增效作用依照Wadley(1967)公式计算不同药剂混合后的协同作用系数:
式中a,b代表1-(2-异硫代氰酰基乙基)-4-甲氧基苯(A),植物精油(B2)两种药剂在混合物中所占比率EC50(A),EC50(B)分别表示实际观测EC50值,EC50(Exp)表示A,B两种药剂按a:b混合后的理论EC50值,EC50(Obs)为A,B两种药剂按a:b混合后的实际观测EC50值。SR>1.5为增效;SR<0.5为拮抗;SR介于0.5和1.5之间表示相加作用。
活性组分A与活性组分B1单剂的室内抗菌活性
活性如表10所示
表10.活性组分A(Y-2)与活性组分B2的室内抗菌活性
Y-2和活性组分B2复配对6种植物病原菌的毒力增效作用如表11所示
表11.Y-2和活性组分B2复配对6种植物病原菌的毒力增效作用(w/w=1:1)
对1:1出现协同的组合进行1:5扩比例测定
表12.Y-2和活性组分B2复配对6种植物病原菌的毒力增效作用(w/w=1:5)
分析表11&表12结果可以看出,1-(2-异硫代氰酰基乙基)-4-甲氧基苯与四种精油的组合效果对油菜菌核菌,立枯丝核菌,番茄灰霉菌的效果都非常好,尤其是Y-2与丁香精油在重量比为1:5时,对番茄灰霉的效果最为显著,SR值达到8.58。
综上所述活性组分A:2-苯乙基异硫氰酸酯(Y-1)与P3和牛至精油展示出了较好的协同增效效果。活性组分A:1-(2-异硫代氰酰基乙基)-4-甲氧基苯(Y-2)与小分子酚类化合物和植物精油的组合均展示出了较为理想的协同增效效果,尤其是当重量比为1:5时,依然对油菜菌核菌,立枯丝核菌和番茄灰霉菌的SR值>1.5,展示出了很好的协同增效效果。
Claims (7)
2.根据权利要求2-苯乙基异硫氰酸酯及1-(2-异硫代氰酰基乙基)-4-甲氧基苯与三种小分子酚类化合物及植物精油在防治油菜菌核病菌引起的病害中的用途。
3.根据权利要求2-苯乙基异硫氰酸酯及1-(2-异硫代氰酰基乙基)-4-甲氧基苯与三种小分子酚类化合物及植物精油在防治立枯丝核菌病菌引起的病害中的用途。
4.根据权利要求2-苯乙基异硫氰酸酯及1-(2-异硫代氰酰基乙基)-4-甲氧基苯与三种小分子酚类化合物及植物精油在防治番茄灰霉菌引起的病害中的用途。
5.根据权利要求2-苯乙基异硫氰酸酯及1-(2-异硫代氰酰基乙基)-4-甲氧基苯与三种小分子酚类化合物及植物精油在防治小麦赤霉菌引起的病害中的用途。
6.根据权利要求2-苯乙基异硫氰酸酯及1-(2-异硫代氰酰基乙基)-4-甲氧基苯与三种小分子酚类化合物及植物精油在防治稻瘟病菌引起的病害中的用途。
7.根据权利要求2-苯乙基异硫氰酸酯及1-(2-异硫代氰酰基乙基)-4-甲氧基苯与三种小分子酚类化合物及植物精油在防治棉花枯萎病菌引起的病害中的用途。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110102271.8A CN112913849A (zh) | 2021-01-26 | 2021-01-26 | 一种含有异硫氰酸酯类化合物的农药组合物及其在防治植物病害中的用途 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110102271.8A CN112913849A (zh) | 2021-01-26 | 2021-01-26 | 一种含有异硫氰酸酯类化合物的农药组合物及其在防治植物病害中的用途 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112913849A true CN112913849A (zh) | 2021-06-08 |
Family
ID=76166121
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110102271.8A Pending CN112913849A (zh) | 2021-01-26 | 2021-01-26 | 一种含有异硫氰酸酯类化合物的农药组合物及其在防治植物病害中的用途 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112913849A (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114375969A (zh) * | 2022-01-06 | 2022-04-22 | 上海耕道农业科技有限公司 | 一种防治马铃薯黑痣病的组合物及其制备方法和应用 |
-
2021
- 2021-01-26 CN CN202110102271.8A patent/CN112913849A/zh active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114375969A (zh) * | 2022-01-06 | 2022-04-22 | 上海耕道农业科技有限公司 | 一种防治马铃薯黑痣病的组合物及其制备方法和应用 |
CN114375969B (zh) * | 2022-01-06 | 2023-08-04 | 上海耕道农业科技有限公司 | 一种防治马铃薯黑痣病的组合物及其制备方法和应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Gavanji et al. | Comparative effect of propolis of honey bee and some herbal extracts on Candida albicans | |
Ekpenyong et al. | Use of Cymbopogon citratus essential oil in food preservation: Recent advances and future perspectives | |
Rusenova et al. | Antimicrobial activities of twelve essential oils against microorganisms of veterinary importance. | |
Sukatta et al. | Antifungal activity of clove and cinnamon oil and their synergistic against postharvest decay fungi of grape in vitro | |
US20130045954A1 (en) | Antimicrobial composition | |
CN103957732A (zh) | 醛、有机酸和有机酸酯的抗微生物混合物 | |
CN107258895A (zh) | 一种基于山苍子油的防腐保鲜组合物 | |
Ikeura et al. | Antimicrobial and antifungal activity of volatile extracts of 10 herb species against Glomerella cingulata | |
CN112913849A (zh) | 一种含有异硫氰酸酯类化合物的农药组合物及其在防治植物病害中的用途 | |
Soni et al. | In-vitro Antibacterial and antifungal activity of select essential oils | |
Ramli et al. | Toxicity analysis of Syzygium polyanthum (Wight) Walp. leaves extract and its stability against different pH and temperature | |
CN112868653A (zh) | 一种含有桧木醇和植物精油及其主要成分的杀菌组合物在防治植物病害和霉菌中的用途 | |
US11510409B2 (en) | Fungicide composition for treating the banana and/or plantain tree | |
Nematollahi et al. | The antibacterial activity of cinnamon essential oil against foodborne bacteria: a mini-review | |
Aćimović et al. | Application of Cymbopogon citratus in Agro-Food Industry. | |
CN107897198B (zh) | 中生菌素和丁子香酚的复配组合物及其应用 | |
CN114304169B (zh) | 一种含有白叶藤碱类似物和小分子酚类化合物的杀菌组合物及其在防治植物病害中的应用 | |
Tayeb et al. | Botanical oils as eco-friendly alternatives for controlling the rice weevil Sitophilus oryzae | |
Al-Ansary et al. | Evaluation of antifungal activity of some natural essential oils against fungal pathogens associated with maize grains. | |
MX2013014626A (es) | Desinfectantes fitoquimicos para lechuga (lactuca sativa). | |
CHRAIBI et al. | Chemical characterization and antimicrobial activity of Moroccan Pelargonium asperum essential oil | |
KR101175693B1 (ko) | 백작약 추출물, 육계 추출물 및 오배자 추출물을 유효성분으로 함유하는 천연 식품 보존제 | |
CN106376642A (zh) | 一种用于猕猴桃果实采后贮藏的生物防腐剂 | |
Naik et al. | Evaluation of essential oil of Cymbopogon distans and Cinnamomum tamala against plant pathogenic fungi | |
KR101176944B1 (ko) | 감초 추출물, 목단피 추출물 및 황련 추출물을 유효성분으로 함유하는 천연 식품 보존제 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |