CN112899213A - Nutrition promoter and application thereof in hericium erinaceus fermentation culture - Google Patents
Nutrition promoter and application thereof in hericium erinaceus fermentation culture Download PDFInfo
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- CN112899213A CN112899213A CN202110280876.6A CN202110280876A CN112899213A CN 112899213 A CN112899213 A CN 112899213A CN 202110280876 A CN202110280876 A CN 202110280876A CN 112899213 A CN112899213 A CN 112899213A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a nutrition promoter and application thereof in hericium erinaceus fermentation culture. The nutrition promoter comprises the following components: inulin, hericium erinaceus powder, triacontanol, nicotinic acid, ethephon, rhubarb extract and water. The nutrition promoter can be used in liquid fermentation of Hericium erinaceus, and can improve mycelium content and Hericium erinaceus polysaccharide content in mycelium. The nutrition promoter is added in the hericium erinaceus liquid fermentation culture method, the content of mycelium polysaccharide obtained by separation under an optimal scheme can reach 18.56%, and the yield is 6-10 times higher than that of the mycelium polysaccharide obtained by traditional liquid fermentation of hericium erinaceus.
Description
Technical Field
The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a nutrition promoter and application thereof in hericium erinaceus fermentation culture.
Background
Hericium erinaceus is rich in minerals, vitamins, unsaturated fatty acids and polysaccharides. The record of the compendium of materia medica states that the hericium erinaceus has mild nature and sweet taste, benefits five internal organs, helps digestion, and has the effects of tonifying spleen, assisting stomach and treating physical weakness. Modern pharmacological studies show that the hericium erinaceus can treat gastric mucosal injury and chronic atrophic gastritis and can remarkably improve the eradication rate of helicobacter pylori and the ulcer healing rate. The active component with most nutritive value is hericium erinaceus polysaccharide which is separated from mycelium and fungus sporocarp and is a high-molecular polymer formed by connecting more than 10 monosaccharides by glycosidic bonds, can regulate cell growth, differentiation and aging, and has close relation with regulating immunity, tonifying spleen and nourishing stomach.
The production sources of the hericium erinaceus include artificial cultivation, solid fermentation and liquid fermentation, wherein the liquid fermentation has the advantages of short period, high production efficiency and the like and is adopted by more and more people. However, liquid fermentation has high requirements on fermentation equipment, harsh fermentation conditions, complex fermentation process and low mycelium production, and the existence of the factors restricts the comprehensive popularization and application of liquid fermentation. Chinese patent CN201010109627.2 discloses a liquid fermentation culture method of Hericium erinaceus, which improves the biological yield of liquid fermentation mycelium of Hericium erinaceus, and the biological content of the obtained mycelium reaches 12g/L, which is 2 times of the biomass of the mycelium obtained by traditional liquid fermentation. However, the content of the polysaccharide in the hericium erinaceus in the mycelium obtained by the method is only 1.65%. Chinese patent CN201210332844.7 discloses a liquid fermentation culture method of medicinal Hericium erinaceus mycelium, and the obtained Hericium erinaceus mycelium contains various anticancer substances, vitamins, amino acids and active cellulase compared with common edible Hericium erinaceus, and has high medicinal value. Although this patent provides a method for increasing the production of various active substances, it does not provide a concept capable of promoting the increase of the content of secondary metabolites of hericium erinaceus.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a novel nutrition promoter. The nutrition promoter can be used in liquid fermentation of Hericium erinaceus, and can improve mycelium content and Hericium erinaceus polysaccharide content in mycelium.
The invention also aims to provide the application of the nutrition promoter in liquid fermentation culture of hericium erinaceus.
The invention also aims to provide a hericium erinaceus liquid fermentation culture method.
The invention also aims to provide a preparation method of the hericium erinaceus crude polysaccharide.
The above object of the present invention is achieved by the following technical solutions:
a nutrition promoter comprises the following components: inulin, hericium erinaceus powder, triacontanol, nicotinic acid, ethephon, rhubarb extract and water.
Inulin, as a fructosyl biomass from jerusalem artichoke, is a soluble dietary fiber, is easily absorbed and utilized by mycelium, has the function of regulating the activity of sugar metabolism enzyme, and can promote the conversion of a carbon source in a culture medium. The Hericium erinaceus powder also contains multiple active ingredients, and has good physiological effects. Triacontanol is a commonly used plant growth regulator, ethephon is a plant hormone, and the inventor finds that the triacontanol can accelerate the growth of hericium erinaceus to a certain extent and promote the generation of various products. The rhubarb extract can participate in the metabolism of microorganisms, so that the hericium erinaceus polysaccharide can be produced in a large scale. The inventor finds that the combination can be used as a nutrition promoter for regulating edible fungi, and has the effects of promoting the growth of hericium erinaceus and accelerating the generation of metabolites (such as hericium erinaceus polysaccharide).
More specifically, the nutrition promoter comprises the following components in percentage by weight: 0.5-2% of inulin, 0.5-2% of hericium erinaceus powder, 0.0001-0.002% of triacontanol, 0.0001-0.002% of nicotinic acid, 0.0001-0.001% of ethephon, 0.0001-0.001% of rhubarb extract and the balance of water.
Preferably, the nutrition promoter comprises the following components in percentage by weight: 1.5-2% of inulin, 1.5-2% of hericium erinaceus powder, 0.001-0.002% of triacontanol, 0.001-0.002% of nicotinic acid, 0.0005-0.001% of ethephon, 0.0005-0.001% of rhubarb extract and the balance of water.
The preparation method of the nutrition promoter comprises the steps of mixing the components except water, adding water and uniformly mixing to obtain the nutrition promoter.
The nutrient promoter is applied to serving as a nutrient supplement for liquid fermentation culture of hericium erinaceus.
The experiment of the inventor proves that the nutrition promoter has the most obvious effect when being added in the liquid fermentation culture stage of the liquid fermentation culture of the hericium erinaceus.
More specifically, the submerged liquid fermentation culture method of the hericium erinaceus comprises the following steps:
s1, activating hericium erinaceus strains;
s2, liquid fermentation of hericium erinaceus: performing liquid fermentation on the activated strain to obtain hericium erinaceus mycelium; s2, adding the nutrition promoter which accounts for 1-5% of the volume of a culture medium used for liquid fermentation when liquid fermentation is carried out.
By adding the nutrition promoter disclosed by the invention into S2, the biomass of the mycelium can be increased, and the polysaccharide content in the mycelium is also increased.
The strain activation and the strain liquid fermentation culture can be carried out according to the conventional method. The aim is to culture the strains in the preservation state step by step to obtain pure and strong cultures, namely to obtain cultures with vigorous activity and enough inoculation quantity, and to prepare for inoculation.
In the present invention, the strain activation method may be performed as follows:
carrying out subculture on the hericium erinaceus strain for 2-3 generations, wherein the subculture temperature is 24-28 ℃, and the culture time is 10-15 d; the activation culture medium is a comprehensive potato culture medium.
Preferably, the composition of the activation medium is preferably as follows: the potato beverage comprises, by weight, 15-30% of a potato extract, 1-3% of glucose and KH2PO4 0.1～0.3％，MgSO4·7H20.1-0.2% of O, 0-0.01% of vitamin B10.005, 1-3% of agar and the balance of water.
More specifically, the preparation method of the potato extract can be as follows: taking 150-300 g of peeled potatoes, cutting into small pieces, adding 1.0L of water, boiling for 15-20 min, filtering out potato pieces, and supplementing the filtrate to 1.0L.
In the present invention, the liquid fermentation culture method may be performed as follows:
and taking a plurality of activated hericium erinaceus strains, inoculating the hericium erinaceus strains into a liquid culture medium, and culturing at the temperature of 24-28 ℃ for 7-14 days at the rotating speed of 130-150 r/min to obtain the liquid fermentation mycelia of the hericium erinaceus.
In the present invention, the nitrogen source of the medium may be a single nitrogen source or a combination of nitrogen sources. The selection of the nitrogen source can be made in accordance with the prior art, and preferably, the nitrogen source can be selected from soybean meal powder. The soybean meal powder can obviously promote the generation amount of polysaccharide, and simultaneously contains certain soybean polypeptide, wherein the soybean polypeptide is soybean hydrolyzed fibrin, and is easier to be absorbed and utilized by hericium erinaceus cells in the growth environment of the hericium erinaceus, so that the growth and metabolism of the hericium erinaceus are promoted.
The inventor further proves that the culture medium compounded with the nitrogen source can more obviously promote the enrichment of the hericium erinaceus polysaccharide through experiments. Therefore, preferably, the nitrogen source of the liquid medium is a built-in nitrogen source. More preferably, the nitrogen source of the liquid culture medium is the compound of soybean meal and yeast extract.
Both soybean meal and yeast extract belong to organic nitrogen sources, wherein nitrogen source substances in the yeast extract mainly exist in the form of easily absorbed protein degradation products, and the degradation products, particularly amino acid, can be directly utilized by organisms through transamination, so that the yeast extract is favorable for thallus growth and is a quick-acting nitrogen source; the nitrogen in the soybean meal exists mainly in the form of macromolecular protein, and is absorbed and utilized by microorganisms after being further degraded into micromolecular peptide and amino acid, and the utilization speed is slow, so that the formation of metabolites is facilitated, and the nitrogen source is a slow-acting nitrogen source. Therefore, the nitrogen source used by the invention is the compound of the soybean meal and the yeast extract, and can control and coordinate the growth period of the thallus and the formation period of the metabolite, thereby achieving the purpose of improving the yield.
Preferably, the composition of the liquid medium is preferably as follows: 1-3% of glucose, 1-2% of starch, 1-2% of soybean meal powder, 1-2% of yeast extract, 0.1-0.3% of phosphate and MgSO (MgSO)4·7H20.1-0.2% of O, 0-0.01% of vitamin B10.005 and the balance of water.
A preparation method of crude polysaccharide of Hericium erinaceus mycelium comprises obtaining Hericium erinaceus mycelium by the liquid fermentation culture method of Hericium erinaceus, drying, grinding, extracting with water, and precipitating with ethanol to obtain crude polysaccharide of Hericium erinaceus mycelium.
In the invention, the source of the hericium erinaceus strain is China Industrial microbial strain preservation management center, and the strain number is CICC 14026.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a novel nutrition promoter, which can improve the biomass of mycelia and simultaneously promote the enrichment of polysaccharide content in hericium erinaceus mycelia to improve the polysaccharide content in the mycelia when being applied to liquid fermentation culture of hericium erinaceus. The content of mycelium polysaccharide obtained by separating mycelium cultured by the nutrition promoter can reach 18.56 percent, and the yield is 6 to 10 times higher than that of the mycelium polysaccharide of the traditional liquid fermented hericium erinaceus.
Drawings
FIG. 1 comparative examples and examples Hericium erinaceus biomass.
FIG. 2 shows the yield of Hericium erinaceus polysaccharide in the comparative example and example.
Detailed Description
The present invention will be further described with reference to specific embodiments, but the present invention is not limited to the examples in any way. The starting reagents employed in the examples of the present invention are, unless otherwise specified, those that are conventionally purchased.
Comparative example 1
A hericium erinaceus strain (strain number: CICC 14026; source: China center for culture Collection of Industrial microorganisms) is prepared from a single nitrogen source and no nutrition promoter. The liquid fermentation culture method comprises the following steps:
s1, strain activation
A strain is subjected to subculture for 3 generations to realize strain activation. The subculture temperature is 26 ℃, and the whole inclined plane can grow over after the culture time is 14 days. The activation culture medium is a comprehensive potato culture medium, and comprises the following components: according to the weight percentage, the potato extract is 20 percent,2% glucose, KH2PO4 0.2％，MgSO4·7H20.15 percent of O, trace vitamin B1, 1.5 percent of agar and the balance of water, and the pH is natural.
Potato extract: removing peel of potato 200g, cutting into small pieces, adding water 1.0L, boiling for 20min, filtering to remove potato pieces, and adding filtrate to 1.0L.
S2, liquid state fermentation culture:
taking a plurality of blocks of hericium erinaceus strains on the activated inclined plane by aseptic operation, inoculating the blocks into a liquid culture medium, and culturing at 26 ℃ at a rotating speed of 130r/min for 10 days to obtain the liquid fermentation mycelium of the hericium erinaceus. The liquid culture medium comprises the following components in percentage by weight: according to weight percentage, 2 percent of glucose, 1 percent of starch, 4 percent of soybean meal powder, 0.2 percent of phosphate and MgSO4·7H20.15% of O, 10.005% of vitamin B and the balance of water. The prepared liquid seed medium was sterilized at 121 ℃ for 15min before use.
After the culture is finished, centrifuging the culture solution to obtain wet mycelium, drying at 55 ℃ to obtain dry mycelium, and weighing to calculate the biomass. Grinding dry Hericium erinaceus mycelium into powder, adding 20 times of water, extracting in 80 deg.C water bath for 3h, centrifuging at 4000r/min for 10min, collecting supernatant, concentrating under reduced pressure at 60 deg.C to 15mL, filtering, centrifuging filtrate, collecting supernatant, adding 4 times of anhydrous ethanol, and standing overnight. And (4) carrying out suction filtration, washing the precipitate with absolute ethyl alcohol, and obtaining the hericium erinaceus mycelium crude polysaccharide. And (3) measuring the content of the crude polysaccharide of the hericium erinaceus mycelium by using a phenol-sulfuric acid method.
In comparative example 1, 7.66g/L biomass was obtained, and the polysaccharide yield was 2.22%.
Wherein, the calculation formula of the polysaccharide yield is as follows:
comparative example 2
Comparative example 2 is a strain of Hericium erinaceus (strain number: CICC 14026; source: China center for Industrial microbial culture Collection) with a compound nitrogen source but without the addition of a nutrient promoter. The liquid fermentation culture method comprises the following steps:
s1, strain activation
A strain is subjected to subculture for 3 generations to realize strain activation. The subculture temperature is 26 ℃, and the whole inclined plane can grow over after the culture time is 14 days. The activation culture medium is a comprehensive potato culture medium, and comprises the following components: according to the weight percentage, the potato extract is 20 percent, the glucose is 2 percent, and the KH is2PO4 0.2％，MgSO4·7H20.15 percent of O, trace vitamin B1, 1.5 percent of agar and the balance of water, and the pH is natural.
Potato extract: removing peel of potato 200g, cutting into small pieces, adding water 1.0L, boiling for 20min, filtering to remove potato pieces, and adding filtrate to 1.0L.
S2, liquid state fermentation culture:
taking a plurality of blocks of hericium erinaceus strains on the activated inclined plane by aseptic operation, inoculating the blocks into a liquid culture medium, and culturing at 26 ℃ at a rotating speed of 130r/min for 10 days to obtain the liquid fermentation mycelium of the hericium erinaceus. The liquid culture medium comprises the following components in percentage by weight: according to weight percentage, 2 percent of glucose, 1 percent of starch, 2 percent of soybean meal, 2 percent of yeast extract, 0.2 percent of phosphate and MgSO4·7H2O: 0.15%, vitamin B1: 0.005% and the balance of water. The prepared seed liquid medium was sterilized at 121 ℃ for 15 min.
After the culture is finished, centrifuging the culture solution to obtain wet mycelium, drying at 55 ℃ to obtain dry mycelium, and weighing to calculate the biomass. Grinding dry Hericium erinaceus mycelium into powder, adding 20 times of water, extracting in 80 deg.C water bath for 3h, centrifuging at 4000r/min for 10min, collecting supernatant, concentrating under reduced pressure at 60 deg.C to 15mL, filtering, centrifuging filtrate, collecting supernatant, adding 4 times of anhydrous ethanol, and standing overnight. And (4) carrying out suction filtration, washing the precipitate with absolute ethyl alcohol, and obtaining the hericium erinaceus mycelium crude polysaccharide. And (3) measuring the content of the crude polysaccharide of the hericium erinaceus mycelium by using a phenol-sulfuric acid method.
In comparative example 2, 10.22g/L biomass was obtained, and the polysaccharide yield was 2.80%. It can be seen that under the condition of not adding a nutrition promoter, the culture medium adopts a compound nitrogen source to obtain more hericium erinaceus mycelium and more polysaccharide.
Example 1
The liquid fermentation culture method, procedure and process conditions of example 1 were the same as those of comparative example 1, i.e., a single nitrogen source was used in the culture medium, except that 1% by volume of a nutrient promoter, which was formulated as follows, was added to the liquid fermentation culture (S2.) relative to the volume of the liquid fermentation culture medium: the composition comprises (by weight) inulin 0.5%, Hericium erinaceus powder 0.5%, triacontanol 0.0001%, nicotinic acid 0.0001%, ethephon 0.0001%, radix et rhizoma Rhei extract 0.0001%, and water in balance.
Through measurement, the biomass of 28.40g/L is obtained in example 1, and the polysaccharide yield is 11.20%. The biomass and polysaccharide yield are both obviously improved. It can be seen that more hericium erinaceus mycelia and more polysaccharides can be obtained with the addition of the nutritional supplement of the present invention.
Example 2
The liquid fermentation culture method, procedure and process conditions of example 2 were the same as those of comparative example 1, i.e., a single nitrogen source was used in the culture medium, except that 3% by volume of a nutrient promoter, which was formulated as follows, was added to the liquid fermentation culture (S2.) relative to the volume of the liquid fermentation culture medium: the traditional Chinese medicine composition comprises, by weight, 1.5% of inulin, 1.5% of hericium erinaceus powder, 0.001% of triacontanol, 0.001% of nicotinic acid, 0.0005% of ethephon, 0.0005% of rheum officinale extract and the balance of water.
According to the determination, 31.30g/L biomass is obtained in example 2, and the yield of polysaccharide is 13.5%. The biomass and polysaccharide yield are both obviously improved. It can be seen that more hericium erinaceus mycelia and more polysaccharides can be obtained with the addition of the nutritional supplement of the present invention.
Example 3
The liquid fermentation culture method, procedure and process conditions of example 3 were the same as those of comparative example 1, i.e., a single nitrogen source was used in the culture medium, except that 5% by volume of a nutrient promoter, which was formulated as follows, was added to the liquid fermentation culture (S2.) relative to the volume of the liquid fermentation culture medium: the traditional Chinese medicine composition comprises, by weight, 2% of inulin, 2% of hericium erinaceus powder, 0.002% of triacontanol, 0.002% of nicotinic acid, 0.001% of ethephon, 0.001% of rhubarb extract and the balance of water.
Through measurement, 35.50g/L biomass is obtained in example 3, and the polysaccharide yield is 14.10%. The biomass and polysaccharide yield are both obviously improved. It can be seen that more hericium erinaceus mycelia and more polysaccharides can be obtained with the addition of the nutritional supplement of the present invention.
Example 4
The liquid fermentation culture method, procedure and process conditions of example 4 were the same as those of comparative example 2, namely, a complex nitrogen source was used in the culture medium, except that a 1% by volume of a nutrient promoter, which was formulated as follows, was added to the liquid fermentation culture (S2.) relative to the volume of the liquid fermentation culture medium: the composition comprises (by weight) inulin 0.5%, Hericium erinaceus powder 0.5%, triacontanol 0.0001%, nicotinic acid 0.0001%, ethephon 0.0001%, radix et rhizoma Rhei extract 0.0001%, and water in balance.
Through measurement, the biomass of 40.10g/L is obtained in example 4, and the polysaccharide yield is 15.00%. The biomass and polysaccharide yield are both obviously improved. It can be seen that more hericium erinaceus mycelia and more polysaccharides can be obtained with the addition of the nutritional supplement of the present invention.
Example 5
The liquid fermentation culture method, procedure and process conditions of example 5 were the same as those of comparative example 2, i.e., a complex nitrogen source was used in the culture medium, except that 3% by volume of a nutrient promoter, based on the volume of the liquid fermentation culture medium, was added to the liquid fermentation culture (S2.), and the nutrient promoter was formulated as follows: the traditional Chinese medicine composition comprises, by weight, 1.5% of inulin, 1.5% of hericium erinaceus powder, 0.001% of triacontanol, 0.001% of nicotinic acid, 0.0005% of ethephon, 0.0005% of rheum officinale extract and the balance of water.
By measurement, 43.50g/L biomass was obtained in example 5, and the polysaccharide yield was 16.50%. The biomass and polysaccharide yield are both obviously improved. It can be seen that more hericium erinaceus mycelia and more polysaccharides can be obtained with the addition of the nutritional supplement of the present invention.
Example 6
The liquid fermentation culture method, procedure and process conditions of example 6 were the same as those of comparative example 2, i.e., a complex nitrogen source was used in the culture medium, except that a nutrient supplement was added to the liquid fermentation culture (S2.) in an amount of 5% by volume relative to the volume of the liquid fermentation culture medium, and the nutrient supplement was formulated as follows: the traditional Chinese medicine composition comprises, by weight, 2% of inulin, 2% of hericium erinaceus powder, 0.002% of triacontanol, 0.002% of nicotinic acid, 0.001% of ethephon, 0.001% of rhubarb extract and the balance of water.
Through measurement, 47.60g/L biomass is obtained in example 6, and the polysaccharide yield is 18.56%. The biomass and polysaccharide yield are both obviously improved. It can be seen that more hericium erinaceus mycelia and more polysaccharides can be obtained with the addition of the nutritional supplement of the present invention.
Comparative example 3
The liquid fermentation culture method, procedure and process conditions of comparative example 3 were the same as those of example 5, i.e., a complex nitrogen source was used in the culture medium, except that the formulation of the nutrient supplement was:
the composition comprises, by weight, 1.5% of inulin, 1.5% of hericium erinaceus powder, 0.001% of triacontanol, 0.0001% of nicotinic acid, 0.0005% of ethephon and the balance of water.
As a result of measurement, comparative example 3 obtained 24.68g/L biomass, and the polysaccharide yield was 9.23%. As can be seen from comparative example 3, although the biomass and polysaccharide yield of the Hericium erinaceus mycelia were slightly improved by adding a part of the components of the nutrition enhancer, the improvement effect was inferior to that of the foregoing embodiment.
Comparative example 4
The liquid fermentation culture method, procedure and process conditions of comparative example 4 were the same as those of example 5 except that the formulation of the nutrient supplement was:
the inulin comprises, by weight, 1.5% of inulin, 0.001% of triacontanol, 0.001% of nicotinic acid, 0.0005% of ethephon, 0.0005% of rhubarb extract and the balance of water.
Through determination, the biomass of the comparative example 4 is 22.86g/L, and the polysaccharide yield is 7.47%. As can be seen from comparative example 4, although the biomass and polysaccharide yield of the Hericium erinaceus mycelia were slightly improved by adding a part of the components of the nutrition enhancer, the improvement effect was inferior to that of the foregoing embodiment.
Comparative example 5
The liquid fermentation culture method, procedure and process conditions of comparative example 5 were the same as those of example 5 except that the formulation of the nutrient supplement was: the hericium erinaceus beverage comprises, by weight, 1.5% of hericium erinaceus powder, 0.001% of triacontanol, 0.001% of nicotinic acid, 0.0005% of ethephon, 0.0005% of rheum officinale extract and the balance of water.
Through measurement, the biomass of the comparative example 5 is 21.52g/L, and the polysaccharide yield is 8.78%. As can be seen from comparative example 5, although the biomass and polysaccharide yield of the mycelia of Hericium erinaceus were slightly improved by adding a part of the components of the nutrient supplement, the improvement effect was inferior to that of the previous embodiment.
Comparative example 6
The liquid fermentation culture method, procedure and process conditions of comparative example 6 were the same as those of example 5 except that the formulation of the nutrient supplement was: the weight percentage of the inulin is 1.5 percent, the hericium erinaceus powder is 2 percent, the nicotinic acid is 0.001 percent, the ethephon is 0.0005 percent, the rhubarb extract is 0.0005 percent, and the balance is water.
Through determination, the biomass of the comparative example 6 is 24.92g/L, and the polysaccharide yield is 9.61%. As can be seen from comparative example 6, although the biomass and polysaccharide yield of the mycelia of Hericium erinaceus were slightly improved by adding a part of the components of the nutrient supplement, the improvement effect was inferior to that of the previous embodiment.
Comparative example 7
The liquid fermentation culture method, procedure and process conditions of comparative example 7 were the same as those of example 5 except that the formulation of the nutrient supplement was: the traditional Chinese medicine composition comprises, by weight, 1.5% of inulin, 1.5% of hericium erinaceus powder, 0.001% of triacontanol, 0.0005% of ethephon, 0.0005% of rheum officinale extract and the balance of water.
Through determination, the biomass of the comparative example 7 is 22.98g/L, and the polysaccharide yield is 7.86%. As can be seen from comparative example 7, although the biomass and polysaccharide yield of the mycelia of Hericium erinaceus were slightly improved by adding a part of the components of the nutrient supplement, the improvement effect was inferior to that of the previous embodiment.
Comparative example 8
The liquid fermentation culture method, procedure and process conditions of comparative example 8 were the same as those of example 5 except that the formulation of the nutrient supplement was: the traditional Chinese medicine composition comprises, by weight, 1.5% of inulin, 1.5% of hericium erinaceus powder, 0.001% of triacontanol, 0.001% of nicotinic acid, 0.0005% of rhubarb extract and the balance of water.
Through determination, the biomass of the comparative example 8 is 25.26g/L, and the polysaccharide yield is 8.12%. As can be seen from comparative example 8, although the biomass and polysaccharide yield of the mycelia of Hericium erinaceus were slightly improved by adding a part of the components of the nutrient supplement, the improvement effect was inferior to that of the previous embodiment.
As can be seen from comparison of comparative examples 3-8 and comparative example 2, the components of the nutrition promoter have promotion effects on the biomass of the hericium erinaceus mycelium and the yield of polysaccharide, but the biomass is below 26g/L, and the yield of polysaccharide is below 10%. Under the same conditions, when the nutrition promoter is used, taking example 5 as an example, the biomass is increased from 20-26 g/L to more than 40g/L, the polysaccharide yield is increased from 9% to 16%, and the increasing effect is obvious.
As can be seen from comparison between the comparative example 1 and the examples 1-3, even if a culture medium with a single nitrogen source is adopted, the biomass of the hericium erinaceus mycelium and the yield of polysaccharide are remarkably improved when the nutrition promoter is added.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. A nutritional promoter is characterized by comprising the following components: inulin, hericium erinaceus powder, triacontanol, nicotinic acid, ethephon, rhubarb extract and water.
2. The nutrition enhancer is characterized by comprising the following components in percentage by weight: 0.5-2% of inulin, 0.5-2% of hericium erinaceus powder, 0.0001-0.002% of triacontanol, 0.0001-0.002% of nicotinic acid, 0.0001-0.001% of ethephon, 0.0001-0.001% of rhubarb extract and the balance of water.
3. A nutritional supplement according to claim 1 or 2, comprising the following ingredients in weight percent: 1.5-2% of inulin, 1.5-2% of hericium erinaceus powder, 0.001-0.002% of triacontanol, 0.001-0.002% of nicotinic acid, 0.0005-0.001% of ethephon, 0.0005-0.001% of rhubarb extract and the balance of water.
4. Use of the nutrient supplement of any one of claims 1 to 3 as a liquid fermentation culture nutrient supplement for hericium erinaceus.
5. A hericium erinaceus liquid fermentation culture method is characterized by comprising the following steps:
s1, activating hericium erinaceus strains;
s2, liquid state fermentation culture: performing liquid fermentation on the activated strain to obtain a hericium erinaceus liquid fermentation mycelium;
s2, when liquid fermentation is carried out, the nutrition promoter as claimed in any one of claims 1 to 3 is added in an amount of 1 to 5% by volume relative to the volume of a medium used for the liquid fermentation.
6. The liquid fermentation culture method of hericium erinaceus according to claim 5, wherein in S1, the hericium erinaceus strain activation is performed as follows: carrying out subculture on the hericium erinaceus strain for 2-3 generations, wherein the subculture temperature is 24-28 ℃, and the culture time is 10-15 d; the activation culture medium is potato culture medium.
7. The liquid fermentation culture method for hericium erinaceus according to claim 6, wherein the activation medium comprises the following components in percentage by weight: potato extract15-30%, glucose 1-3%, KH2PO4 0.1～0.3％，MgSO4·7H20.1-0.2% of O, 0-0.01% of vitamin B10.005, 1-3% of agar and the balance of water.
8. The hericium erinaceus liquid fermentation culture method according to claim 5, wherein in S2, liquid fermentation is performed according to the following method: and taking a plurality of activated hericium erinaceus strains, inoculating the hericium erinaceus strains into a liquid culture medium, and culturing at the temperature of 24-28 ℃ for 7-14 days at the rotating speed of 130-150 r/min to obtain the liquid fermentation mycelia of the hericium erinaceus.
9. The liquid fermentation culture method of hericium erinaceus according to claim 8, wherein the nitrogen source of the liquid culture medium is a compound nitrogen source.
10. A preparation method of crude polysaccharide of Hericium erinaceus mycelium is characterized in that the crude polysaccharide of Hericium erinaceus mycelium is obtained by adopting the liquid fermentation culture method of Hericium erinaceus as claimed in any one of claims 5 to 9, and then is dried, ground, extracted with water and precipitated with alcohol to obtain the crude polysaccharide of Hericium erinaceus mycelium.
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| CN110122188A (en) * | 2019-06-28 | 2019-08-16 | 广西壮族自治区农业科学院微生物研究所 | Edible mushroom cultivation nutrition promotor and its application |
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