CN112898342A - Benfotiamine derivative, preparation method and pharmaceutical composition thereof - Google Patents
Benfotiamine derivative, preparation method and pharmaceutical composition thereof Download PDFInfo
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- CN112898342A CN112898342A CN202110120995.5A CN202110120995A CN112898342A CN 112898342 A CN112898342 A CN 112898342A CN 202110120995 A CN202110120995 A CN 202110120995A CN 112898342 A CN112898342 A CN 112898342A
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- BTNNPSLJPBRMLZ-LGMDPLHJSA-N benfotiamine Chemical class C=1C=CC=CC=1C(=O)SC(/CCOP(O)(O)=O)=C(/C)N(C=O)CC1=CN=C(C)N=C1N BTNNPSLJPBRMLZ-LGMDPLHJSA-N 0.000 title claims abstract description 31
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims abstract description 15
- 125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 claims abstract description 8
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 46
- 208000024827 Alzheimer disease Diseases 0.000 claims description 11
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical group [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 5
- 230000032683 aging Effects 0.000 claims description 3
- 230000002265 prevention Effects 0.000 claims description 2
- 125000005843 halogen group Chemical group 0.000 abstract description 28
- 125000001309 chloro group Chemical group Cl* 0.000 abstract description 10
- 125000001153 fluoro group Chemical group F* 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 5
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 abstract description 5
- 125000001246 bromo group Chemical group Br* 0.000 abstract description 4
- 150000001875 compounds Chemical class 0.000 abstract description 4
- 230000005764 inhibitory process Effects 0.000 abstract description 4
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 abstract description 4
- -1 2-methyl-4-amino-5-pyrimidinyl Chemical group 0.000 description 143
- 239000000047 product Substances 0.000 description 71
- 238000012360 testing method Methods 0.000 description 60
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 48
- 238000005160 1H NMR spectroscopy Methods 0.000 description 47
- 238000001819 mass spectrum Methods 0.000 description 26
- 238000006467 substitution reaction Methods 0.000 description 25
- RUQIUASLAXJZIE-UHFFFAOYSA-N 3-methoxybenzoyl chloride Chemical compound COC1=CC=CC(C(Cl)=O)=C1 RUQIUASLAXJZIE-UHFFFAOYSA-N 0.000 description 23
- PVWNFAGYFUUDRC-UHFFFAOYSA-N 4-amino-5-formamidomethyl-2-methylpyrimidine Chemical compound CC1=NC=C(CNC=O)C(N)=N1 PVWNFAGYFUUDRC-UHFFFAOYSA-N 0.000 description 23
- 125000004093 cyano group Chemical group *C#N 0.000 description 23
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 23
- 238000004949 mass spectrometry Methods 0.000 description 23
- 239000007787 solid Substances 0.000 description 23
- 230000015572 biosynthetic process Effects 0.000 description 22
- 125000002252 acyl group Chemical group 0.000 description 21
- 239000011550 stock solution Substances 0.000 description 21
- 125000000217 alkyl group Chemical group 0.000 description 20
- 125000005245 nitryl group Chemical group [N+](=O)([O-])* 0.000 description 20
- 239000007858 starting material Substances 0.000 description 20
- 238000003786 synthesis reaction Methods 0.000 description 20
- 125000003545 alkoxy group Chemical group 0.000 description 19
- 125000005415 substituted alkoxy group Chemical group 0.000 description 19
- 125000000547 substituted alkyl group Chemical group 0.000 description 19
- 238000004166 bioassay Methods 0.000 description 18
- 229960002873 benfotiamine Drugs 0.000 description 13
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229910052801 chlorine Inorganic materials 0.000 description 6
- 229910052731 fluorine Inorganic materials 0.000 description 6
- 125000003277 amino group Chemical group 0.000 description 5
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 5
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Natural products O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 125000003396 thiol group Chemical group [H]S* 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 108010064397 amyloid beta-protein (1-40) Proteins 0.000 description 4
- 108010064539 amyloid beta-protein (1-42) Proteins 0.000 description 4
- PASDCCFISLVPSO-UHFFFAOYSA-N benzoyl chloride Chemical compound ClC(=O)C1=CC=CC=C1 PASDCCFISLVPSO-UHFFFAOYSA-N 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000012091 fetal bovine serum Substances 0.000 description 4
- 125000001183 hydrocarbyl group Chemical group 0.000 description 4
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 238000005481 NMR spectroscopy Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- HZSAJDVWZRBGIF-UHFFFAOYSA-M thiamine(1+) monophosphate(2-) Chemical compound CC1=C(CCOP([O-])([O-])=O)SC=[N+]1CC1=CN=C(C)N=C1N HZSAJDVWZRBGIF-UHFFFAOYSA-M 0.000 description 3
- QYNVGXKRERVLCV-UHFFFAOYSA-N 2-fluorobenzenecarbothioic s-acid Chemical compound OC(=S)C1=CC=CC=C1F QYNVGXKRERVLCV-UHFFFAOYSA-N 0.000 description 2
- BHJWUUSAMQADIT-UHFFFAOYSA-N 2-methylbenzenecarbothioic s-acid Chemical compound CC1=CC=CC=C1C(O)=S BHJWUUSAMQADIT-UHFFFAOYSA-N 0.000 description 2
- RCHAKLCYTZRINN-UHFFFAOYSA-N 2-nitrobenzenecarbothioic s-acid Chemical compound OC(=S)C1=CC=CC=C1[N+]([O-])=O RCHAKLCYTZRINN-UHFFFAOYSA-N 0.000 description 2
- CBZUINSNIXJMHO-UHFFFAOYSA-N 3-chlorobenzenecarbothioic s-acid Chemical compound SC(=O)C1=CC=CC(Cl)=C1 CBZUINSNIXJMHO-UHFFFAOYSA-N 0.000 description 2
- QASTUZSTAJITJN-UHFFFAOYSA-N 3-fluorobenzenecarbothioic s-acid Chemical compound OC(=S)C1=CC=CC(F)=C1 QASTUZSTAJITJN-UHFFFAOYSA-N 0.000 description 2
- LIPOOEDNQBASFM-UHFFFAOYSA-N 3-methoxybenzenecarbothioic s-acid Chemical compound COC1=CC=CC(C(S)=O)=C1 LIPOOEDNQBASFM-UHFFFAOYSA-N 0.000 description 2
- KUUBHOLGHXMYGR-UHFFFAOYSA-N 3-methylbenzenecarbothioic s-acid Chemical compound CC1=CC=CC(C(O)=S)=C1 KUUBHOLGHXMYGR-UHFFFAOYSA-N 0.000 description 2
- JGAVFTBPHJHVBD-UHFFFAOYSA-N 3-nitrobenzenecarbothioic s-acid Chemical compound [O-][N+](=O)C1=CC=CC(C(S)=O)=C1 JGAVFTBPHJHVBD-UHFFFAOYSA-N 0.000 description 2
- LBVWMBBKFFQMRX-UHFFFAOYSA-N 4-chlorobenzenecarbothioic s-acid Chemical compound SC(=O)C1=CC=C(Cl)C=C1 LBVWMBBKFFQMRX-UHFFFAOYSA-N 0.000 description 2
- PEQZEAWOMNXGCD-UHFFFAOYSA-N 4-fluorobenzenecarbothioic s-acid Chemical compound FC1=CC=C(C(S)=O)C=C1 PEQZEAWOMNXGCD-UHFFFAOYSA-N 0.000 description 2
- YGAPOICUMNPIPF-UHFFFAOYSA-N 4-methoxybenzenecarbothioic s-acid Chemical compound COC1=CC=C(C(S)=O)C=C1 YGAPOICUMNPIPF-UHFFFAOYSA-N 0.000 description 2
- FPRSKHDBFYFDHU-UHFFFAOYSA-N 4-nitrobenzenecarbothioic s-acid Chemical compound [O-][N+](=O)C1=CC=C(C(S)=O)C=C1 FPRSKHDBFYFDHU-UHFFFAOYSA-N 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 2
- OKKJLVBELUTLKV-MZCSYVLQSA-N Deuterated methanol Chemical compound [2H]OC([2H])([2H])[2H] OKKJLVBELUTLKV-MZCSYVLQSA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 206010039966 Senile dementia Diseases 0.000 description 2
- 229930003451 Vitamin B1 Natural products 0.000 description 2
- 150000001335 aliphatic alkanes Chemical group 0.000 description 2
- 125000002511 behenyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
- 125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000001149 cognitive effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000012065 filter cake Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- GRVDJDISBSALJP-UHFFFAOYSA-N methyloxidanyl Chemical group [O]C GRVDJDISBSALJP-UHFFFAOYSA-N 0.000 description 2
- 230000004770 neurodegeneration Effects 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 239000011691 vitamin B1 Substances 0.000 description 2
- 235000010374 vitamin B1 Nutrition 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 125000006218 1-ethylbutyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000001088 1-naphthoyl group Chemical group C1(=CC=CC2=CC=CC=C12)C(=O)* 0.000 description 1
- 125000001637 1-naphthyl group Chemical group [H]C1=C([H])C([H])=C2C(*)=C([H])C([H])=C([H])C2=C1[H] 0.000 description 1
- QRHUZEVERIHEPT-UHFFFAOYSA-N 2,6-difluorobenzoyl chloride Chemical group FC1=CC=CC(F)=C1C(Cl)=O QRHUZEVERIHEPT-UHFFFAOYSA-N 0.000 description 1
- RJLSQWUWBMUMPX-UHFFFAOYSA-N 2,6-dimethylbenzenecarbothioic S-acid Chemical compound CC1=C(C(=S)O)C(=CC=C1)C RJLSQWUWBMUMPX-UHFFFAOYSA-N 0.000 description 1
- CFLAYISSADVCJH-UHFFFAOYSA-N 2,6-dimethylbenzoyl chloride Chemical group CC1=CC=CC(C)=C1C(Cl)=O CFLAYISSADVCJH-UHFFFAOYSA-N 0.000 description 1
- OFQUPAIEWXOKTE-UHFFFAOYSA-N 2-bromobenzenecarbothioic s-acid Chemical compound SC(=O)C1=CC=CC=C1Br OFQUPAIEWXOKTE-UHFFFAOYSA-N 0.000 description 1
- NZCKTGCKFJDGFD-UHFFFAOYSA-N 2-bromobenzoyl chloride Chemical group ClC(=O)C1=CC=CC=C1Br NZCKTGCKFJDGFD-UHFFFAOYSA-N 0.000 description 1
- MDKAAWDKKBFSTK-UHFFFAOYSA-N 2-ethoxybenzoyl chloride Chemical group CCOC1=CC=CC=C1C(Cl)=O MDKAAWDKKBFSTK-UHFFFAOYSA-N 0.000 description 1
- RAAGZOYMEQDCTD-UHFFFAOYSA-N 2-fluorobenzoyl chloride Chemical group FC1=CC=CC=C1C(Cl)=O RAAGZOYMEQDCTD-UHFFFAOYSA-N 0.000 description 1
- PQJHUOAGYZQONV-UHFFFAOYSA-N 2-iodobenzenecarbothioic s-acid Chemical compound OC(=S)C1=CC=CC=C1I PQJHUOAGYZQONV-UHFFFAOYSA-N 0.000 description 1
- MVIVDSWUOGNODP-UHFFFAOYSA-N 2-iodobenzoyl chloride Chemical group ClC(=O)C1=CC=CC=C1I MVIVDSWUOGNODP-UHFFFAOYSA-N 0.000 description 1
- GPZXFICWCMCQPF-UHFFFAOYSA-N 2-methylbenzoyl chloride Chemical group CC1=CC=CC=C1C(Cl)=O GPZXFICWCMCQPF-UHFFFAOYSA-N 0.000 description 1
- 125000001216 2-naphthoyl group Chemical group C1=C(C=CC2=CC=CC=C12)C(=O)* 0.000 description 1
- 125000001622 2-naphthyl group Chemical group [H]C1=C([H])C([H])=C2C([H])=C(*)C([H])=C([H])C2=C1[H] 0.000 description 1
- BWWHTIHDQBHTHP-UHFFFAOYSA-N 2-nitrobenzoyl chloride Chemical group [O-][N+](=O)C1=CC=CC=C1C(Cl)=O BWWHTIHDQBHTHP-UHFFFAOYSA-N 0.000 description 1
- 125000000094 2-phenylethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])([H])* 0.000 description 1
- BLFSJRRKPANUBS-UHFFFAOYSA-N 3-bromobenzenecarbothioic S-acid Chemical compound SC(=O)C1=CC=CC(Br)=C1 BLFSJRRKPANUBS-UHFFFAOYSA-N 0.000 description 1
- PBOOZQFGWNZNQE-UHFFFAOYSA-N 3-bromobenzoyl chloride Chemical group ClC(=O)C1=CC=CC(Br)=C1 PBOOZQFGWNZNQE-UHFFFAOYSA-N 0.000 description 1
- WHIHIKVIWVIIER-UHFFFAOYSA-N 3-chlorobenzoyl chloride Chemical group ClC(=O)C1=CC=CC(Cl)=C1 WHIHIKVIWVIIER-UHFFFAOYSA-N 0.000 description 1
- AAOVIYJFIFTBPL-UHFFFAOYSA-N 3-ethenylbenzenecarbothioic S-acid Chemical compound SC(=O)C1=CC=CC(C=C)=C1 AAOVIYJFIFTBPL-UHFFFAOYSA-N 0.000 description 1
- ASAANMJKRKZQQI-UHFFFAOYSA-N 3-ethenylbenzoyl chloride Chemical group ClC(=O)C1=CC=CC(C=C)=C1 ASAANMJKRKZQQI-UHFFFAOYSA-N 0.000 description 1
- SYVNVEGIRVXRQH-UHFFFAOYSA-N 3-fluorobenzoyl chloride Chemical group FC1=CC=CC(C(Cl)=O)=C1 SYVNVEGIRVXRQH-UHFFFAOYSA-N 0.000 description 1
- YHOYYHYBFSYOSQ-UHFFFAOYSA-N 3-methylbenzoyl chloride Chemical group CC1=CC=CC(C(Cl)=O)=C1 YHOYYHYBFSYOSQ-UHFFFAOYSA-N 0.000 description 1
- NXTNASSYJUXJDV-UHFFFAOYSA-N 3-nitrobenzoyl chloride Chemical group [O-][N+](=O)C1=CC=CC(C(Cl)=O)=C1 NXTNASSYJUXJDV-UHFFFAOYSA-N 0.000 description 1
- AHKOLAZRIZPPJJ-UHFFFAOYSA-N 4-bromobenzenecarbothioic s-acid Chemical compound SC(=O)C1=CC=C(Br)C=C1 AHKOLAZRIZPPJJ-UHFFFAOYSA-N 0.000 description 1
- DENKGPBHLYFNGK-UHFFFAOYSA-N 4-bromobenzoyl chloride Chemical group ClC(=O)C1=CC=C(Br)C=C1 DENKGPBHLYFNGK-UHFFFAOYSA-N 0.000 description 1
- RKIDDEGICSMIJA-UHFFFAOYSA-N 4-chlorobenzoyl chloride Chemical group ClC(=O)C1=CC=C(Cl)C=C1 RKIDDEGICSMIJA-UHFFFAOYSA-N 0.000 description 1
- OPUDMVWSHKGPCH-UHFFFAOYSA-N 4-ethenylbenzenecarbothioic s-acid Chemical compound SC(=O)C1=CC=C(C=C)C=C1 OPUDMVWSHKGPCH-UHFFFAOYSA-N 0.000 description 1
- DGZXVEAMYQEFHB-UHFFFAOYSA-N 4-ethenylbenzoyl chloride Chemical group ClC(=O)C1=CC=C(C=C)C=C1 DGZXVEAMYQEFHB-UHFFFAOYSA-N 0.000 description 1
- XLWQUESMILVIPR-UHFFFAOYSA-N 4-ethoxybenzoyl chloride Chemical group CCOC1=CC=C(C(Cl)=O)C=C1 XLWQUESMILVIPR-UHFFFAOYSA-N 0.000 description 1
- CZKLEJHVLCMVQR-UHFFFAOYSA-N 4-fluorobenzoyl chloride Chemical group FC1=CC=C(C(Cl)=O)C=C1 CZKLEJHVLCMVQR-UHFFFAOYSA-N 0.000 description 1
- CLTLFKPWCCGIAX-UHFFFAOYSA-N 4-hydroxybenzenecarbothioic s-acid Chemical compound OC(=S)C1=CC=C(O)C=C1 CLTLFKPWCCGIAX-UHFFFAOYSA-N 0.000 description 1
- YIPHYCQSJTXLFM-UHFFFAOYSA-N 4-hydroxybenzoyl chloride Chemical group OC1=CC=C(C(Cl)=O)C=C1 YIPHYCQSJTXLFM-UHFFFAOYSA-N 0.000 description 1
- SKDHHIUENRGTHK-UHFFFAOYSA-N 4-nitrobenzoyl chloride Chemical group [O-][N+](=O)C1=CC=C(C(Cl)=O)C=C1 SKDHHIUENRGTHK-UHFFFAOYSA-N 0.000 description 1
- 208000037259 Amyloid Plaque Diseases 0.000 description 1
- 108010090849 Amyloid beta-Peptides Proteins 0.000 description 1
- 102000013455 Amyloid beta-Peptides Human genes 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- OSEVUZSYFBUMOR-UHFFFAOYSA-N C(C)OC1=CC=C(C(=S)O)C=C1 Chemical compound C(C)OC1=CC=C(C(=S)O)C=C1 OSEVUZSYFBUMOR-UHFFFAOYSA-N 0.000 description 1
- KNWDOHAPGMVYLN-UHFFFAOYSA-N CCOC1=CC=CC(C(O)=S)=C1 Chemical compound CCOC1=CC=CC(C(O)=S)=C1 KNWDOHAPGMVYLN-UHFFFAOYSA-N 0.000 description 1
- IAZDPXIOMUYVGZ-WFGJKAKNSA-N Dimethyl sulfoxide Chemical compound [2H]C([2H])([2H])S(=O)C([2H])([2H])[2H] IAZDPXIOMUYVGZ-WFGJKAKNSA-N 0.000 description 1
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N Heavy water Chemical compound [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- HRKDQZNYQYFGDD-UHFFFAOYSA-N OC(C(C1)(F)SC2=C1C=CC(F)=C2)=O Chemical compound OC(C(C1)(F)SC2=C1C=CC(F)=C2)=O HRKDQZNYQYFGDD-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
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- 238000002835 absorbance Methods 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 125000002009 alkene group Chemical group 0.000 description 1
- 125000002355 alkine group Chemical group 0.000 description 1
- MXMOTZIXVICDSD-UHFFFAOYSA-N anisoyl chloride Chemical group COC1=CC=C(C(Cl)=O)C=C1 MXMOTZIXVICDSD-UHFFFAOYSA-N 0.000 description 1
- 125000001124 arachidoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000001204 arachidyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000003710 aryl alkyl group Chemical group 0.000 description 1
- 125000002102 aryl alkyloxo group Chemical group 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 125000003910 behenoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
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- 159000000007 calcium salts Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 125000000753 cycloalkyl group Chemical group 0.000 description 1
- 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
- 125000002933 cyclohexyloxy group Chemical group C1(CCCCC1)O* 0.000 description 1
- 125000001511 cyclopentyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
- 125000001887 cyclopentyloxy group Chemical group C1(CCCC1)O* 0.000 description 1
- 125000003074 decanoyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C(*)=O 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 125000002704 decyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
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- 125000005448 ethoxyethyl group Chemical group [H]C([H])([H])C([H])([H])OC([H])([H])C([H])([H])* 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 125000002485 formyl group Chemical group [H]C(*)=O 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 125000000755 henicosyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000003104 hexanoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000003707 hexyloxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 1
- 230000006951 hyperphosphorylation Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 125000002510 isobutoxy group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])O* 0.000 description 1
- 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 1
- 125000003253 isopropoxy group Chemical group [H]C([H])([H])C([H])(O*)C([H])([H])[H] 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 125000000400 lauroyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 229910003002 lithium salt Inorganic materials 0.000 description 1
- 159000000002 lithium salts Chemical class 0.000 description 1
- 125000000040 m-tolyl group Chemical group [H]C1=C([H])C(*)=C([H])C(=C1[H])C([H])([H])[H] 0.000 description 1
- 125000000628 margaroyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000002960 margaryl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
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- 230000003340 mental effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 125000001419 myristoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000001421 myristyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000006606 n-butoxy group Chemical group 0.000 description 1
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000003506 n-propoxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 210000002682 neurofibrillary tangle Anatomy 0.000 description 1
- 125000001196 nonadecyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000001402 nonanoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000001400 nonyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000006611 nonyloxy group Chemical group 0.000 description 1
- 230000005311 nuclear magnetism Effects 0.000 description 1
- 125000003261 o-tolyl group Chemical group [H]C1=C([H])C(*)=C(C([H])=C1[H])C([H])([H])[H] 0.000 description 1
- 125000002801 octanoyl group Chemical group C(CCCCCCC)(=O)* 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000005447 octyloxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 1
- 125000001037 p-tolyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)C([H])([H])[H] 0.000 description 1
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000001991 pathophysiological effect Effects 0.000 description 1
- 125000002958 pentadecyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000006340 pentafluoro ethyl group Chemical group FC(F)(F)C(F)(F)* 0.000 description 1
- 125000003538 pentan-3-yl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000004115 pentoxy group Chemical group [*]OC([H])([H])C([H])([H])C([H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- 125000000951 phenoxy group Chemical group [H]C1=C([H])C([H])=C(O*)C([H])=C1[H] 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 125000004368 propenyl group Chemical group C(=CC)* 0.000 description 1
- 239000006215 rectal suppository Substances 0.000 description 1
- 229940100618 rectal suppository Drugs 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 125000005920 sec-butoxy group Chemical group 0.000 description 1
- 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 125000003548 sec-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 125000003696 stearoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000007470 synaptic degeneration Effects 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 102000013498 tau Proteins Human genes 0.000 description 1
- 108010026424 tau Proteins Proteins 0.000 description 1
- 125000004213 tert-butoxy group Chemical group [H]C([H])([H])C(O*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- CZDYPVPMEAXLPK-UHFFFAOYSA-N tetramethylsilane Chemical group C[Si](C)(C)C CZDYPVPMEAXLPK-UHFFFAOYSA-N 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 125000002469 tricosyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000002889 tridecyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000000876 trifluoromethoxy group Chemical group FC(F)(F)O* 0.000 description 1
- 125000002023 trifluoromethyl group Chemical group FC(F)(F)* 0.000 description 1
- 125000000297 undecanoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000002948 undecyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000003774 valeryl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/645—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having two nitrogen atoms as the only ring hetero atoms
- C07F9/6509—Six-membered rings
- C07F9/6512—Six-membered rings having the nitrogen atoms in positions 1 and 3
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Neurosurgery (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Neurology (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Hospice & Palliative Care (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Psychiatry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a series of benfotiamine derivatives, a preparation method and a pharmaceutical composition thereof, when a benzene ring is only ortho-substituted by halogen atoms or ethoxy groups, or only meta-substituted by bromine atoms, chlorine atoms, fluorine atoms or nitro groups, or only para-substituted by chlorine atoms, methoxy groups or nitro groups, the compounds have obvious inhibition effect on A beta 40 and A beta 42, and further, when the benzene ring is only ortho-substituted by fluorine atoms or bromine atoms, the compounds have outstanding inhibition effect on the A beta 40 and the A beta 42.
Description
The application of the invention is a divisional application of an invention application with the application date of 2017, 6 and 26, and the application number of 201710494135.1, and the invention name of the invention is 'benfotiamine derivative, a preparation method and a pharmaceutical composition thereof'.
Technical Field
The invention belongs to the field of medical chemistry, and particularly relates to a benfotiamine derivative, a preparation method and a pharmaceutical composition thereof.
Background
Alzheimer's Disease (AD), commonly known as senile dementia, is a progressive neurodegenerative disease with cognitive and behavioral disorders as the main clinical manifestations, and is the most common senile dementia, mainly manifested as impaired recognition ability and rapid decline of memory function. The main pathophysiological features are the formation of senile plaques by β -amyloid (a β) deposits in the brain, the formation of neurofibrillary tangles by hyperphosphorylation of tau proteins, disturbances of brain glucose metabolism and neuronal/synaptic loss. Due to long course of disease and poor self-care ability of life of patients, serious mental and economic burden is brought to families and society. However, there is no drug that can prevent or delay the development of the disease in the world, and the drugs for treating AD that are currently marketed are only symptomatic drugs, and can only control or improve cognitive and functional symptoms for a while, and cannot prevent or delay the progression of the disease.
Benfotiamine, chemical name S-2- [ [ (2-methyl-4-amino-5-pyrimidinyl) methyl ] formamido ] -5-phosphonoxy-2, 3-pentene-3-thiol benzoate, molecular formula C19H23N4O6PS, can improve the defect of low bioavailability of water-soluble vitamin B1, and improve the concentration of vitamin B1 in blood and tissues, thereby improving the curative effect. The existing research on benfotiamine mainly focuses on the synthesis method and crystal form of benfotiamine and the research on the application of benfotiamine in medicines. Although recent studies show that benfotiamine can be used for preparing a pharmaceutical composition for treating alzheimer's disease, such as patent No. cn200710041571.x discloses that benfotiamine is contained in the pharmaceutical composition for treating alzheimer's disease, no benfotiamine derivative and its pharmaceutical use, particularly the related research report of the benfotiamine derivative for alzheimer's disease, have been discovered for a while.
Disclosure of Invention
The invention aims to provide the following benfotiamine derivatives, a preparation method and a technical scheme of a pharmaceutical composition thereof:
benfotiamine derivatives having the following structure (1),
wherein R is1Is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R2is a hydrogen atom,Halogen atom, nitro group, cyano group, sulfonic group, amino group, carboxyl group, hydroxyl group, mercapto group, alkyl group, substituted alkyl group, alkoxy group, substituted alkoxy group, or acyl group;
R3is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R4is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R5is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
and, R1、R2、R3、R4And R5At least one of which is not a hydrogen atom.
Preferably, said R is1、R2、R3、R4And R5Only one of them is not a hydrogen atom, and the others are all hydrogen atoms.
Preferably, said R is2、R3、R4And R5Is a hydrogen atom, R1Is a halogen atom or an ethoxy group.
Preferably, said R is2、R3、R4And R5Is a hydrogen atom, R1Is fluorine atom or bromine atom.
Preferably, said R is1、R3、R4And R5Is a hydrogen atom, R2Is bromine atom, chlorine atom, fluorine atom or nitro.
Preferably, said R is1、R2、R4And R5 is a hydrogen atom, R3Is chlorine atom, methoxyl or nitryl.
The preparation method of the benfotiamine derivative is characterized in that the benfotiamine phosphate derivative is prepared by reacting the benfotiamine phosphate shown in the formula (1a) with the benzoyl chloride shown in the formula (1 b);
wherein R is1Is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R2is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R3is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R4is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R5is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
and, R1、R2、R3、R4And R5At least one of which is not a hydrogen atom.
Preferably, said R is1、R2、R3、R4And R5Only one of them is not a hydrogen atom, and the others are all hydrogen atoms.
Preferably, said R is2、R3、R4And R5 is a hydrogen atom, R1Is a halogen atom or an ethoxy group.
Preferably, said R is2、R3、R4And R5 is a hydrogen atom, R1Is fluorine atom or bromine atom.
Preferably, said R is1、R3、R4And R5Is a hydrogen atom, R2Is a bromine atom,Chlorine atom, fluorine atom or nitro group.
Preferably, said R is1、R2、R4And R5 is a hydrogen atom, R3Is chlorine atom, methoxyl or nitryl.
A pharmaceutical composition comprising any one of the benfotiamine derivatives or salts thereof.
Preferably, the pharmaceutical composition is used for preparing a medicament for preventing and treating alzheimer disease or aging.
Compared with the prior art, the invention provides a series of benfotiamine derivatives, further, when the benzene ring is only substituted by halogen atoms or ethoxy groups at ortho positions, or only substituted by bromine atoms, chlorine atoms, fluorine atoms or nitro groups at meta positions to be substituted by chlorine atoms, methoxy groups or nitro groups at para positions, the compounds have obvious inhibition effect on A beta 40 and A beta 42, and further, when the benzene ring is substituted by fluorine atoms or bromine atoms at ortho positions, the compounds have outstanding inhibition effect on the A beta 40 and the A beta 42.
Detailed Description
The benfotiamine derivative has the following structure (1),
wherein R is1Is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R2is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R3is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R4is hydrogen atom, halogen atom, nitro group, cyano group, sulfonic group, amino group, carboxyl group, hydroxyl group, mercapto group, alkyl group, or a derivative thereofSubstituted hydrocarbyl, hydrocarbyloxy, substituted hydrocarbyloxy, or acyl;
R5is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
and, R1、R2、R3、R4And R5At least one of which is not a hydrogen atom.
In the present invention, the hydrocarbon group includes a straight-chain, branched or cyclic hydrocarbon group, which may be an alkane group, an alkene group or an alkyne group, but is preferably an alkane group, specifically, for example, methyl, ethyl, vinyl, propenyl, n-propyl, isopropyl, n-butyl, sec-butyl, tert-butyl, isobutyl, pentyl, 1-ethylpropyl, 1-methylbutyl, cyclopentyl, hexyl, 1-methylpentyl, 1-ethylbutyl, cyclohexyl, 2-heptyl, octyl, nonyl, decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, aralkyl, eicosyl, heneicosyl, docosyl, tricosyl, phenyl, 2-methylphenyl, 3-methylphenyl, etc, 4-methylphenyl, 1-naphthyl, 2-naphthyl, benzyl, 2-phenylethyl or the like.
In the present invention, the substituted hydrocarbon group includes halogen atom substitution, nitro group substitution, cyano group substitution, sulfonic group substitution, amino group substitution, carboxyl group substitution, hydroxyl group substitution, mercapto group substitution, and the like of the above-mentioned hydrocarbon group, and specifically includes, for example, methoxyethyl group, ethoxyethyl group, butoxyethyl group, trifluoromethyl group, pentafluoroethyl group, and the like.
In the present invention, the hydrocarbyloxy group includes a straight-chain, branched-chain or cyclic hydrocarbyloxy group, and specifically, for example, methoxy group, ethoxy group, n-propoxy group, isopropoxy group, n-butoxy group, sec-butoxy group, tert-butoxy group, isobutoxy group, pentyloxy group, 1-ethylpropoxy group, 1-methylbutoxy group, cyclopentoxy group, hexyloxy group, 1-methylpentyloxy group, 1-ethylbutoxy group, cyclohexyloxy group, 2-heptyloxy group, octyloxy group, nonyloxy group, decyloxy group, undecyloxy group, dodecyloxy group, tridecyloxy group, tetradecyloxy group, pentadecyloxy group, hexadecyloxy group, heptadecyloxy group, octadecyloxy group, nonadecyloxy group, aralkyloxy group, eicosyloxy group, heneicosyloxy group, docosyl group, tricosyloxy group, phenoxy group, 2-, 4-methylphenoxy, 1-naphthyloxy, 2-naphthyloxy, benzyloxy, 2-phenethyloxy, etc.
In the present invention, the substituted hydrocarbyloxy group includes halogen atom substitution, nitro group substitution, cyano group substitution, sulfonic group substitution, amino group substitution, carboxyl group substitution, hydroxyl group substitution, mercapto group substitution, and the like of the above hydrocarbyloxy group, and specifically includes methoxyethoxy group, ethoxyethoxy group, butoxyethoxy group, trifluoromethoxy group, pentafluoroethoxy group, and the like.
In the present invention, the acyl group includes various kinds of hydrocarbon acyl groups or various kinds of substituted hydrocarbon acyl groups, and the substitution includes halogen atom substitution, nitro group substitution, cyano group substitution, sulfonic group substitution, amino group substitution, carboxyl group substitution, hydroxyl group substitution or mercapto group substitution, etc., specifically, for example, formyl group, acetyl group, n-propionyl group, isopropionyl group, n-butyryl group, sec-butyryl group, tert-butyryl group, isobutyryl group, valeryl group, 1-ethylpropionyl group, 1-methylbutyryl group, cyclopentoyl group, hexanoyl group, 1-methylpentanoyl group, 1-ethylbutyryl group, cyclohexanoyl group, 2-heptanoyl group, octanoyl group, nonanoyl group, decanoyl group, undecanoyl group, dodecanoyl group, tridecanoyl group, tetradecanoyl group, pentadecanoyl group, hexadecanoyl group, heptadecanoyl group, octadecanoyl group, nonadecanoyl group, aralkanoyl group, etc, Eicosanoyl, heneicosanoyl, docosanoyl, tricosanoyl, benzoyl, 2-methylbenzoyl, 3-methylbenzoyl, 4-methylbenzoyl, 1-naphthoyl, 2-naphthoyl, benzoyl, 2-phenylacetyl, methoxyacetyl, ethoxyacetyl, butoxyacetyl, trifluoroformyl, or pentafluoroacetyl, and the like.
In view of the inhibitory effect of the benfotiamine derivative on A β 40 and A β 42, the R is preferably selected1、R2、R3、R4And R5Wherein only one of the groups is not a hydrogen atom and the others are all hydrogen atoms, and more preferably R is as defined above2、R3、R4And R5Is a hydrogen atom, R1Is a halogen atom or an ethoxy group, or the R1、R3、R4And R5Is a hydrogen atom, R2Is a bromine atom, a chlorine atom, a fluorine atom or a nitro group, or said R1、R2、R4And R5 is a hydrogen atom, R3Is a chlorine atom, a methoxy group or a nitro group, more preferably the R2、R3、R4And R5 is a hydrogen atom, R1Is fluorine atom or bromine atom.
The invention also provides a preparation method of the benfotiamine derivative, which is prepared by reacting the benfotiamine phosphate shown in the formula (1a) with the benzoyl chloride shown in the formula (1 b);
wherein the content of the first and second substances,
R1is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R2is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R3is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R4is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
R5is hydrogen atom, halogen atom, nitryl, cyano, sulfonic group, amino, carboxyl, hydroxyl, sulfydryl, alkyl, substituted alkyl, alkoxy, substituted alkoxy or acyl;
and, R1、R2、R3、R4And R5At least one of which is not a hydrogen atom.
The preparation method of the benfotiamine derivative can refer to the method for preparing benfotiamine through the reaction of thiamine phosphate and benzoyl chloride in the prior art, for example, the benfotiamine phosphate derivative is prepared through the experimental condition method disclosed in EP 2918593A 1, the thiamine phosphate shown in the formula (1a) is dissolved in water, the temperature is cooled to 0-5 ℃, a 30% sodium hydroxide solution is dropwise added, the pH value is adjusted to 11-12, and the stirring is carried out for 1-2 hours. Dripping benzoyl chloride shown in the formula (1b) at 0-5 ℃, and controlling and adjusting the pH value to be 11-12 in the dripping process. And reacting for 1-3 hours at 5-10 ℃ after the dropwise addition is finished. Dropwise adding concentrated hydrochloric acid to adjust the pH value to be 3-4, adding ethyl acetate, stirring, filtering, and drying a filter cake to obtain a product.
The preparation method of benfotiamine derivative of the invention is shown in the specification, p1、R2、R3、R4And R5The specific limitations of (A) are as described above.
Further, the present invention also provides a pharmaceutical composition comprising the aforementioned benfotiamine derivative or a salt thereof, preferably a pharmaceutical composition for the preparation of a medicament for the prevention and treatment of alzheimer's disease or aging. The salt is pharmaceutically acceptable salt, such as lithium salt, sodium salt, potassium salt or calcium salt. The composition can be made into tablet, powder, spray, injection, powder for injection, rectal suppository or skin patch (transdermal administration) by conventional method.
Examples
Description of the tests of the invention:
nuclear magnetism (1H NMR): NMR shifts (. delta.) are given in units of 10-6 (ppm). NMR was measured using a Bruker AVANCE-500 NMR spectrometer using deuterated dimethyl sulfoxide (DMSO-d6) and deuterated methanol (CD)3OD), deuterated water (D)2O), and the internal standard is Tetramethylsilane (TMS).
Mass Spectrum (MS): MS was determined using an Agilent (ESI) mass spectrometer (manufacturer: Agilent, model: Agilent 6110).
1. Biological assay
Test materials and methods
(1) The BCA protein concentration determination kit is purchased from Biyun, the A beta 40 and A beta 42 detection kits are purchased from wako, the cell culture related reagents except Fetal Bovine Serum (FBS) are purchased from Gibico, and the FBS is purchased from Shanghai Prolon Biotechnology research and development Limited.
(2) HEK293APP/sw overexpressing cell culture: the cells were cultured in 48-well plates in DMEM (containing 10% FBS, 100ug/ml G418(Geneticin, Geneticin) and double antibody), and at 70% cell density, 4mM stock solution of the test sample (prepared by dissolving the test sample in DMEM) was diluted to 400. mu.M in DMEM, and 500. mu.L of the test sample was added to each well, and the cells were cultured for 24 hours.
(3) Adding BCA reagent into the culture solution supernatant, incubating for 30min at room temperature, measuring the absorbance of each well at OD570nm of the microplate reader, and calculating the total protein concentration according to the protein standard curve. Taking supernatant to measure the concentration of Abeta 40 and Abeta 42, adding the supernatant into a coated 96-well plate 4, incubating overnight, removing and washing reagent, adding HRP (horse radish oxidase) labeled antibody 4, incubating for 2h, removing and washing reagent, adding TMB color solution, incubating at room temperature for 30min, adding stop solution to stop reaction, measuring the light absorption value of each well at an microplate reader OD450nm, respectively calculating the concentration of Abeta 40 and Abeta 42 according to the standard curve of Abeta 40 and Abeta 42, and finally adjusting the concentration of Abeta 40 and Abeta 42 by using the total protein concentration to obtain the final concentration.
Example 1
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphonoxy) pen-2-en-3-yl) 3-methoxybenzothioate, (Z) -S- (2- (N- - ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 3-methoxybenzenethioate 1-1 Synthesis:
dissolving thiamine phosphate 1a (38g, 0.09 mol) in water (103g, 5.7mol), cooling to 0-5 ℃, dropwise adding 30% sodium hydroxide solution (87.3g, 0.65mol), adjusting the pH value to 11-12, stirring for 1.5 hours, dropwise adding 3-methoxybenzoyl chloride (20.4g, 0.12mol) at 0-5 ℃, controlling the pH value to 11-12 in the dropwise adding process, reacting for 2 hours at 5-10 ℃ after the dropwise adding is finished, dropwise adding concentrated hydrochloric acid (34g, 0.33mol) to adjust the pH value to 3-4, adding 50mL ethyl acetate, stirring for 16 hours, filtering, and drying a filter cake to obtain a product 1-1(11g, white-like solid).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the product 1-1, and the results are shown below, and a sample stock solution prepared from the product 1-1 was subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：497.1[M+1]
1H NMR(DMSO-d6)δ7.85(d，1H)，7.78(d，1H)，7.40(t，1H)，7.30(d，1H)，7.25(d，1H)，7.13(d，1H)，4.45-4.25(m，2H)，3.88-3.75(m，5H)，2.75-2.65(m，2H)，2.25(s，3H)，2.15(s，3H)。
Example 2
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphonoxy) pen-2-en-3-yl) 4-ethoxybenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 4-ethoxybenzenethiol ester 1-2 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 4-ethoxybenzoyl chloride to give the product 1-2(1.1g, off-white solid).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1-2, and the results were as follows, and a test stock solution prepared from the products 1-2 was subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：511.1[M+1]
1H NMR(DMSO-d6)δ7.85(d，1H)，7.78(d，1H)，7.65(d，2H)，7.00(d，2H)，4.50-4.35(m，2H)，4.20-4.15(m，2H)，3.80-3.70(m，2H)，2.75-2.65(m，2H)，2.25(s，3H)，2.15(s，3H)，1.30(t，3H)。
Example 3
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphonooxy) pen-2-en-3-yl) 4-hydroxybenezothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 4-hydroxybenzenethioate 1-3 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 4-hydroxybenzoyl chloride to give the product 1-3(0.2g, off-white solid).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 3, and the results were as follows, and the results of biological tests were performed on test stock solutions prepared from the products 1 to 3, and are shown in Table 1.
MS m/z(ESI)：483.1[M+1]
1H NMR(DMSO-d6)δ7.90(s，1H)，7.85(s，1H)，7.61(d，2H)，6.85(d，2H)，4.53-4.31(m，2H)，3.87-3.78(m，2H)，2.76-2.64(m，2H)，2.30(s，3H)，2.17(s，3H)。
Example 4
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphonooxy) pen-2-en-3-yl) 2, 6-difluorobenzothiophenate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl)2, 6-difluorobenzenethiol ester 1-4:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 2, 6-difluorobenzoyl chloride to give the products 1-4(2.2g, off-white solid).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 4, and the results were as follows, and the results of biological tests were performed on test stock solutions prepared from the products 1 to 4, and are shown in Table 1.
MS m/z(ESI)：503.0[M+1]
1H NMR(DMSO-d6)δ7.85(d，2H)，7.65(d，1H)，7.25(d，2H)，4.65-4.35(m，2H)，3.80-3.70(m，2H)，2.75-2.65(m，2H)，2.25(s，3H)，2.15(s，3H)。
Example 5
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphonooxy) pen-2-en-3-yl) 3-bromobenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 3-bromobenzothiolate 1-5 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 3-bromobenzoyl chloride to give the products 1-5(1.7g, as off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 5, and the results were as follows, and the results of bioassay using the test stock solutions prepared from the products 1 to 5 are shown in Table 1.
MS m/z(ESI)：545.0[M+1]
1H NMR(DMSO-d6)δ7.93-7.89(m，3H)，7.76-7.72(d，2H)，7.48(d，1H)，4.65-4.35(m，2H)，3.87(d，2H)，2.70(d，2H)，2.28(s，3H)，2.20(s，3H)。
Example 6
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphonooxy) pen-2-en-3-yl) 3-nitrobenzothioate, (Z) -S- (2- (N- - ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 3-nitrobenzenethioate 1-6 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 3-nitrobenzoyl chloride to give the products 1-6(0.2g, as off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1-6, and the results were as follows, and the results of bioassay using the test stock solutions prepared from the products 1-6 are shown in Table 1.
MS m/z(ESI)：512.2[M+1]
1H NMR(DMSO-d6)δ8.50(d，1H)，8.35(d，1H)，8.15(d，1H)，7.90(d，1H)，7.87(d，1H)7.75(t，1H)，4.50-4.35(m，2H)，3.87-3.75(d，2H)，2.70(d，2H)，2.20(s，6H)。
Example 7
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 4-fluorobenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 4-fluorobenzenethioate 1-7 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 4-fluorobenzoyl chloride to give the products 1-7(11g, as off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 7, and the results were as follows, and the results of bioassay using the test stock solutions prepared from the products 1 to 7 are shown in Table 1.
MS m/z(ESI)：483.1[M+1]
1H NMR(DMSO-d6)δ7.93(s，1H)，7.90(s，1H)，7.79(d，2H)，7.40(d，2H)，4.54-4.37(m，2H)，3.86(d，2H)，2.70(d，2H)，2.25(s，3H)，2.20(s，3H)。
Example 8
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 3-fluorobenzothioate, (Z) -S- (2- (N- - ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 3-fluorobenzenethioate 1-8 Synthesis:
using the synthetic route of example 1, the starting 3-methoxybenzoyl chloride was replaced with 3-fluorobenzoyl chloride to give the products 1-8(11g, off-white solid).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 8, and the results were as follows, and the test stock solutions prepared from the products 1 to 8 were subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：485.1[M+1]
1H NMR(DMSO-d6)δ7.88(s，1H)，7.84(s，1H)，7.58(d，3H)，7.41(d，1H)，4.51-4.35(m，2H)，3.84(d，2H)，2.71(d，2H)，2.23(s，3H)，2.19(s，3H)。
Example 9
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 2-fluorobenzothioate, (Z) -S- (2- (N- - ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 2-fluorobenzenethioate 1-9 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 2-fluorobenzoyl chloride to give the products 1-9(11g, as off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 9, and the results were as follows, and the results of bioassay using the test stock solutions prepared from the products 1 to 9 are shown in Table 1.
MS m/z(ESI)：485.1[M+1]
1H NMR(DMSO-d6)δ7.94(s，1H)，7.89(s，1H)，7.67(d，2H)，7.37(d，2H)，4.55-4.36(m，2H)，3.85(d，2H)，2.71(d，2H)，2.26(s，3H)，2.20(s，3H)。
Example 10
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 2-methylbenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 2-methylbenzenethioate 1-10 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 2-methylbenzoyl chloride to give the products 1-10(11g, off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 10, and the results were as follows, and the results of biological tests were performed by formulating test stock solutions with the products 1 to 10, and are shown in Table 1.
MS m/z(ESI)：481.1[M+1]
1H NMR(DMSO-d6)δ7.89(s，1H)，7.87(s，1H)，7.56(d，1H)，7.48(d，1H)，7.33(d，2H)，4.55-4.36(m，2H)，3.85(d，2H)，2.78(d，2H)，2.31(s，3H)，2.27(s，3H)，2.19(s，3H)。
Example 11
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 4-nitrobenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 4-nitrobenzenethioate 1-11 Synthesis:
using the synthetic route of example 1, the starting material, 3-methoxybenzoyl chloride, was replaced with 4-nitrobenzoyl chloride to afford the products 1-11(10g, off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 11, and the results were as follows, and the results of biological tests were performed on test stock solutions prepared from the products 1 to 11, and are shown in Table 1.
MS m/z(ESI)：512.2[M+1]
1H NMR(DMSO-d6)δ8.35(s，1H)，8.33(s，1H)，7.94(d，1H)，7.92(d，2H)，7.87(s，1H)，4.56-4.34(m，2H)，3.89-3.75(m，2H)，2.74(d，2H)，2.21(s，6H)。
Example 12
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 4-methoxybenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 4-methoxybenzenethioate 1-12 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 4-methoxybenzoyl chloride to give the products 1-12(10g, off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 12, and the results were as follows, and test stocks prepared from the products 1 to 12 were subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：497.1[M+1]
1H NMR(DMSO-d6)δ7.91(s，1H)，7.85(s，1H)，7.71(d，2H)，7.06(d，2H)，4.56-4.34(m，2H)，3.87-3.75(m，5H)，2.70(s，2H)，2.30(s，3H)，2.18(s，3H)。
Example 13
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphoroxy) pen-2-en-3-yl) 3-chlorobenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 3-chlorobenzenethioate 1-13 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 3-chlorobenzoyl chloride to give the products 1-13(10g, off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 13, and the results were as follows, and the test stock solutions prepared from the products 1 to 13 were subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：501.1[M+1]
1H NMR(DMSO-d6)δ7.91(s，2H)，7.78(d，1H)，7.69(d，1H)，7.63(s，1H)，7.57(d，1H)，7.45-7.20(m，2H)，4.53-4.35(m，2H)，3.87-3.75(m，2H)，2.71(d，2H)，2.25(s，3H)，2.20(s，3H)。
Example 14
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 3-methylbenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 3-methylbenzenethioate 1-14 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 3-methylbenzoyl chloride to give the products 1-14(11g, off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 14, and the results were as follows, and test stocks prepared from the products 1 to 14 were subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：481.1[M+1]
1H NMR(DMSO-d6)δ7.90(s，1H)，7.87(s，1H)，7.52-7.49(m，3H)，7.43(d，1H)，4.55-4.36(m，2H)，3.86(d，2H)，2.71(s，2H)，2.38(s，3H)，2.28(s，3H)，2.18(s，3H)。
Example 15
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphoroxy) pen-2-en-3-yl) 4-chlorobenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 4-chlorobenzenethioate 1-15 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 4-chlorobenzoyl chloride to give the products 1-15(0.3g, off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1-15, and the results are shown below, and the results of biological tests were performed on test stock solutions prepared from the products 1-15, and are shown in Table 1.
MS m/z(ESI)：501.0[M+1]
1H NMR(DMSO-d6)δ7.88(s，1H)，7.84(s，1H)，7.71(d，2H)，7.61(d，2H)，4.50-4.35(m，2H)，3.87(d，2H)，2.71(d，2H)，2.22(s，3H)，2.19(s，3H)。
Example 16
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphoroxy) pen-2-en-3-yl) 3-ethoxybenzothioate, (Z) -S- (2- (N- - ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 3-ethoxybenzenethiol ester 1-16:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 2-ethoxybenzoyl chloride to give the products 1-16(0.2g, off-white solid).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1-16, and the results were as follows, and test stocks prepared from the products 1-16 were subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：511.1[M+1]
1H NMR(DMSO-d6)δ8.06(s，1H)，7.92(s，1H)，7.56(d，2H)，7.17(d，1H)，7.03(t，1H)，4.58-4.38(m，2H)，4.18(q，2H)，3.93-3.80(m，2H)，2.68(s，2H)，2.19(s，6H)，1.35(t，3H)。
Example 17
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 4-bromobenzothioate, (Z) -S- (2- (N- - ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl)4-bromobenzothioate 1-17 Synthesis:
using the synthetic route of example 1, the starting 3-methoxybenzoyl chloride was replaced with 4-bromobenzoyl chloride to give the products 1-17(3.5g, as off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 17, and the results were as follows, and the test stock solutions prepared from the products 1 to 17 were subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：54/.0[M+1]
1H NMR(DMSO-d6)δ8.08(d，1H)，7.90(s，1H)，7.86(d，1H)，7.76(d，1H)，7.73(d，1H)7.63(d，1H)，4.50-4.35(m，2H)，3.87-3.75(m，2H)，2.70(t，2H)，2.24(s，3H)，2.19(s，3H)。
Example 18
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 2-bromobenzothioate, (Z) -S- (2- (N- - ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl)2-bromobenzothioate 1-19 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 2-bromobenzoyl chloride to give the products 1-18(3.5g, as off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 18, and the results were as follows, and test stocks prepared from the products 1 to 18 were subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：593.0[M+1]
1H NMR(DMSO-d6)δ7.98-7.93(m，3H)，7.51(t，1H)，7.42(d，1H)，7.32(d，1H)，4.64-4.32(m，2H)，3.87-3.75(m，2H)，2.78(s，2H)，2.20(s，3H)，2.30(s，3H)
Example 19
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphoroxy) pen-2-en-3-yl) 2-iodobenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 2-iodobenzenethiol ester 1-20 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 2-iodobenzoyl chloride to give the products 1-19(0.06g, as off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1-19, and the results are shown below, in which test stock solutions were prepared from the products 1-19 and subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：547.0[M+1]
1H NMR(DMSO-d6)δ7.96(s，1H)，7.93(s，1H)，7.74(s，1H)，7.50(s，3H)，4.60-4.35(m，2H)，3.87-3.75(m，2H)，2.70(d，2H)，2.31(s，3H)，2.20(s，3H)。
Example 20
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 3-vinylbenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 3-vinylbenzenethiol ester 1-20 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 3-vinylbenzoyl chloride to give the products 1-20(0.8g, off-white solids).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1 to 20, and the results were as follows, and the results of biological tests were performed on test stock solutions prepared from the products 1 to 20, and are shown in Table 1.
MS m/z(ESI)：492.12[M+1]
1H NMR(DMSO-d6)δ7.93(s，1H)，7.89(s，1H)，7.83(d，1H)，7.72(s，1H)，7.63(d，1H)，7.53(t，1H)，6.80(q，1H)，5.93(d，1H)，5.38(d，1H)，4.48(br，2H)，3.87(d，2H)，2.72(s，2H)，2.27(s，3H)，2.20(s，3H)。
Example 21
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 4-vinylbenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl) 4-vinylbenzenethiol ester 1-21 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 4-vinylbenzoyl chloride to give the products 1-21(2g, off-white solids).
The products 1-21 were subjected to nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests, and the results were as follows,
the products 1-21 were used to formulate test stock solutions for biological testing, the results of which are shown in Table 1.
MS m/z(ESI)：492.12[M+1]
1H NMR(DMSO-d6)δ7.88-7.87(m，2H)，7.70(d，2H)，7.62(d，2H)，7.13(br，2H)，6.85(q，1H)，6.04(d，1H)，5.45(d，1H)，4.43(br，2H)，3.82(d，2H)，2.71(s，2H)，2.23(s，3H)，2.18(s，3H)
Example 22
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 2-nitrobenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) carboxamide) -5- (phosphonooxy) pent-2-en-3-yl) 2-nitrobenzenethioate 1-22 Synthesis:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 2-nitrobenzoyl chloride to give the products 1-22(50mg, off-white solid).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1-22, and the results were as follows, and test stocks prepared from the products 1-22 were subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：512.2[M+1]
1H NMR(DMSO-d6)δ8.12(d，1H)，7.93(s，1H)，7.92(s，1H)，7.87-7.83(m，2H)，7.63(d，1H)，7.29(br，1H)，4.49(br，2H)，3.89(d，2H)，2.74(s，2H)，2.25(s，3H)，2.20(s，3H)
Example 23
(Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamido) -5- (phosphooxy) pen-2-en-3-yl) 2, 6-dimethylbenzothioate, (Z) -S- (2- (N- ((4-amino-2-methylpyrimidin-5-yl) methyl) formamide) -5- (phosphonooxy) pent-2-en-3-yl)2, 6-dimethylbenzenethioate 1-23:
using the synthetic route of example 1, the starting material 3-methoxybenzoyl chloride was replaced with 2, 6-dimethylbenzoyl chloride to afford the products 1-23(18mg, off-white solid).
Nuclear magnetic (1H NMR) and Mass Spectrometry (MS) tests were performed on the products 1-23, and the results are shown below, in which test stock solutions were prepared from the products 1-23 and subjected to bioassay, and the results are shown in Table 1.
MS m/z(ESI)：495.1[M+1]
1H NMR(DMSO-d6)δ7.97(d，2H)，7.26(s，1H)，7.08(s，2H)，4.85(br，2H)，3.86(s，2H)，2.76(s，2H)，2.32(s，3H)，2.19(s，3H)
Comparative example 1
The bioassay was carried out without adding the stock solutions of the test products and with the medium as a blank, and the results are shown in Table 1.
Comparative example 2
The result of the biological test using the benfotiamine to prepare the stock solution of the test article is shown in Table 1.
Table 1 shows the content of A beta 40 and A beta 42 protein secreted by APP/293 cells treated by benfotiamine derivatives
Although the present invention is disclosed above, the present invention is not limited thereto. Various changes and modifications may be effected therein by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (3)
1. Benfotiamine derivative with the structural formula as shown in the formula (I)
Wherein R2, R3, R4 and R5 are hydrogen atoms, and R1 is an ethoxy group; alternatively, the first and second electrodes may be,
and R1, R3, R4 and R5 are hydrogen atoms, and R2 is a bromine atom or a nitro group.
2. A pharmaceutical composition comprising a benfotiamine derivative having a structure of the following formula (1) or a salt thereof,
wherein R2, R3, R4 and R5 are hydrogen atoms, and R1 is an ethoxy group; alternatively, the first and second electrodes may be,
and R1, R3, R4 and R5 are hydrogen atoms, and R2 is a bromine atom or a nitro group.
3. The pharmaceutical composition according to claim 2, characterized in that it is used for the preparation of a medicament for the prevention and treatment of alzheimer's disease or of aging.
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