CN112891257B

CN112891257B - Mosquito-repellent composition and application thereof

Info

Publication number: CN112891257B
Application number: CN202110109252.8A
Authority: CN
Inventors: 孙金媛; 赵崇江; 王俊林; 陈意平; 佘奕河
Original assignee: Runben Biotechnology Co ltd
Current assignee: Runben Biotechnology Co ltd
Priority date: 2021-01-26
Filing date: 2021-01-26
Publication date: 2022-11-15
Anticipated expiration: 2041-01-26
Also published as: CN112891257A

Abstract

The invention discloses a mosquito repellent composition, and belongs to the technical field of daily necessities. The mosquito repellent composition comprises mosquito repellent ester and balsam seed oil. The invention also discloses a mosquito repellent containing the mosquito repellent composition and a preparation method thereof, and the mosquito repellent can enhance the mosquito repellent effect and the itching relieving effect by mutually matching the components; meanwhile, no alcohol is added into the mosquito repellent, and the preparation process is simple; the system is uniform, transparent and clear, and the hydrolysis degree of the anophelifuge ester is low.

Description

Mosquito-repellent composition and application thereof

Technical Field

The invention relates to a mosquito repellent composition and application thereof, and belongs to the technical field of daily necessities.

Background

The mosquito repellent ester is chemically named as acetamido butyl ester, is partially dissolved in water, has high safety, and is commonly used as a mosquito repellent. The products containing the anophelifuge are mainly prepared into sprays, emulsions and the like, and the emulsions are mainly sprays due to the inconvenient use form. The anophelifuge ester has poor water solubility and high degradation rate in water, so most of the anophelifuge spray is wrapped by an alcohol system or a microcapsule.

Patent CN201911412675.6 provides a mosquito repellent ester long-acting repellent, which is composed of the following raw materials in percentage by weight: 5-20% of anophelifuge, 5-20% of polyvinylpyrrolidone, 15-40% of absolute ethyl alcohol, 5-20% of polyethylene glycol-400, 10-30% of alpha-pyrrolidone and the balance of deionized water; aims to solve the problems that the prior mosquito repellent is directly smeared on the surface of skin, wastes time and labor during application and is easy to cause allergy. Patent CN106137780B provides a microcapsule containing anophelifuge, mosquito repellent water and a preparation method thereof, in the invention, chitosan and polyglutamic acid are used for wrapping the anophelifuge, and the mosquito repellent water is separated from skin, so that irritation to the skin is reduced, slow release of the mosquito repellent is realized, and the mosquito repellent effect is durable.

The skin of infants is more fragile than adults, the skin is irritated by adding alcohol, the skin is dry and dehydrated after long-term use, and the mosquito repellent product containing alcohol brings the risk of alcohol allergy and is not suitable for long-term use. The microcapsule has larger grain diameter which is in micron level, is easy to generate precipitation for a spraying system with smaller viscosity, is not beneficial to the release of effective components, and simultaneously, the prepared product generally presents milky white and is not beautiful.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the mosquito repellent composition which does not contain alcohol and has good itching relieving effect and the application thereof.

In order to achieve the purpose, the invention adopts the technical scheme that: a mosquito repellent composition comprises 6-15 parts by weight of anophelifuge and 0.01-3 parts by weight of oleum Olivarum.

The mosquito repellent ester is also called deet and Yimeining, is colorless to light yellow transparent liquid at normal temperature, has no toxic or side effect on skin and mucosa, and has no allergy and permeability; the anophelifuge ester has stable chemical properties, can be used under different climatic conditions, and has long mosquito repelling effect time. The mosquito repellent ester is used for seeking a target according to the smell given out by a human body, such as exhaled gas and the smell given out by skin, through an olfactory system, and has the function of forming a barrier on the surface of the human body, so that the smell given out by the human body is isolated, the olfactory system of the mosquito is paralyzed, the smell induction of the mosquito is interfered, and the mosquito repellent effect is realized. The bitter oil tree seed oil is relatively viscous oil with fruity flavor, is golden yellow, is rich in various fatty acids, and also contains limonin compounds, sterols and tannins; the oleum Olivarum has effects of relieving itching, calming and relieving swelling. The anophelifuge ester and the balsam seed oil are compounded, so that the content of the anophelifuge ester is reduced, the synergistic effect is realized, and the mosquito repelling effect is achieved.

As a preferred embodiment of the mosquito repellent composition, the mosquito repellent composition comprises 6-10 parts by weight of mosquito repellent ester and 0.01-1 part by weight of oleum Olivarum. The anophelifuge ester and the balsam pear seed oil can be better matched with each other in the proportion, and the mosquito repelling effect is better.

As a preferred embodiment of the mosquito repellent composition, the mosquito repellent composition also comprises 0.02-0.1 part by weight of hydroxyphenylpropionamide benzoic acid.

Hydroxyphenylpropionamide benzoic acid, also known as dihydroavenanthramide D, is an active ingredient of oats. The avenanthramides have multifunctional anti-inflammatory activity, including inhibiting the degradation of keratinized nuclear factor NF-kB-alpha directly related to inflammation, and preventing the phosphorylation of p65 protein subunit on the nuclear factor NF-kB, thereby blocking the generation process of cell inflammation; reducing the occurrence of inflammatory immune response and the reaction of neurodermatitis in the skin. The avenanthramides can inhibit the activity of TNF-alpha induced NF-kB degrading enzyme, reduce inflammatory factors and cause the release of IL-8 in atopic dermatitis. At low concentrations of 30ppm, avenanthramides can greatly reduce the number of itching episodes in contact and neurodermatitis. The avenanthramides have potent anti-inflammatory and antipruritic properties and are potent non-steroidal anti-inflammatory antipruritic natural ingredients.

In addition, the invention also provides a mosquito repellent containing the mosquito repellent composition.

As a preferred embodiment of the mosquito repellent, the mosquito repellent comprises the following components in parts by weight: 6-15 parts of anophelifuge ester, 0.01-3 parts of oleum Olivarum, 1-10 parts of main surfactant, 3-20 parts of cosurfactant, 0.02-0.1 part of hydroxyphenylpropionamide benzoic acid, 0.3-1 part of preservative, 0.1-0.3pH buffer and the balance of water. The mosquito repellent prepared by the formula has good mosquito repelling effect and itching relieving effect.

As a preferred embodiment of the mosquito repellent, the mosquito repellent comprises the following components in parts by weight: 7 parts of anophelifuge ester, 0.2 part of balsam seed oil, 3 parts of main surfactant, 10 parts of cosurfactant, 0.05 part of hydroxyphenylpropionamide benzoic acid, 0.6 part of preservative, 0.2pH value buffer and the balance of water. When the proportion is adopted, the mosquito repellent has the best mosquito repellent effect and itching relieving effect.

In a preferred embodiment of the mosquito repellent according to the present invention, the primary surfactant is one of sodium cocoamidopropyl PG-dimethyl ammonium chloride phosphate, sodium lauroyl oat amino acid, sodium lauroyl glutamate, sodium lauroyl sarcosinate, sodium cocoyl glutamate, and sodium lauroyl lysine.

Cocamidopropyl-PG-dimethyl ammonium chloride phosphate (natural bionic phospholipid) is a derivative of phospholipid, has good affinity with skin, has a certain repairing function on damaged skin and hair, can supplement phospholipid and grease lost by the skin, and is an extremely mild amphoteric surfactant. Sodium lauroyl oat amino acid, sodium lauroyl glutamate, sodium lauroyl sarcosinate, sodium cocoyl glutamate and sodium lauroyl lysine belong to amino acid surfactants, and the amino acid surfactants not only have good degradability, antibacterial property and safety, but also have no allergy. And due to the special structure, the amphoteric surfactant has many excellent properties of good water solubility, strong salt resistance, pH responsiveness and the like.

In a preferred embodiment of the mosquito repellent according to the present invention, the co-surfactant is at least one of propylene glycol, methyl propylene glycol, butylene glycol, and pentylene glycol.

Propylene glycol is a transparent, colorless, viscous substance, and belongs to a small molecule moisture-retaining component, and can retain water in the stratum corneum. The butanediol is a mild, fresh and cool solution without viscosity, prevents the volatilization of effective components in the mosquito repellent liquid, has no harm to skin after being used, and can also destroy the living environment of bacteria. The pentanediol is an important intermediate of the bactericide propiconazole, and has excellent moisturizing performance and an antiseptic effect.

As a preferred embodiment of the mosquito repellent of the present invention, the preservative is at least one of p-hydroxyacetophenone, hexanediol, caprylyl glycol, and caprylyl hydroxamic acid.

The p-hydroxyacetophenone is mild, free of additive and free of irritant preservative, has the functions of resisting oxidation, tranquilizing and relieving, and has a synergistic effect when being compounded with other preservatives. Hexanediol is a diol, belongs to a rapidly volatile solvent, and is completely soluble in water. And can be used as antibacterial agent with antibacterial effect. The caprylyl glycol is a good humectant, has a bacteriostatic function, is a skin conditioner, can play a role in moisturizing and softening, and achieves the effects of mildness and corrosion prevention. The caprylyl hydroximic acid belongs to coconut oil derivatives, has high-efficiency and selective chelating capacity on ferrous ions and ferric ions, and has limited growth of mould in an environment with limited ferric ions; meanwhile, the optimal carbon chain length capable of promoting the degradation of the cell membrane structure is provided; therefore, has stronger antibacterial ability.

In a third aspect, the invention also provides a preparation method of the mosquito repellent, which comprises the following steps:

(1) Adding water and a pH buffering agent into a stirring pot, and adjusting the pH value of the solution to 6.5-7;

(2) Adding anophelifuge, oleum Olivarum, major surfactant, co-surfactant, and hydroxyphenylpropionamide benzoic acid into the stirring pot, and stirring to obtain mixture;

(3) And adding the preservative into the mixture and uniformly stirring to obtain the mosquito repellent.

The anophelifuge prepared by the method is transparent and clear, has a uniform system, and has low hydrolysis degree.

The invention has the beneficial effects that: the invention provides a mosquito repellent composition and application thereof, and the mosquito repellent composition can increase the mosquito repellent effect and the itching relieving effect; the mosquito repellent prepared from the mosquito repellent composition has no alcohol, and the preparation process is simple; the mosquito repellent is transparent and clear, has a uniform system and has a good mosquito repellent effect; and the hydrolysis degree of the anophelifuge in the anophelifuge is low.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.

The compositions of the mosquito repellent compositions and the mosquito repellent agents described in examples 1 to 4 and comparative examples 1 to 6 are shown in table 1.

The preparation method of the mosquito repellent described in the examples 1 to 4 and the comparative examples 1 to 6 comprises the following steps:

TABLE 1

Example 5

The heat storage stability of the mosquito repellents described in examples 1 to 4 and comparative examples 1 to 6 was measured.

The test method comprises the following steps: placing the mosquito repellent in a constant temperature box at (54 +/-2) DEG for 14 days, taking out, wiping the bottle body, and observing whether the liquid is layered/turbid or has other changes. The test results are shown in table 2.

TABLE 2

As can be seen from Table 2, the mosquito repellent of the present invention has good thermal stability without containing alcohol. However, when the surfactant is less than the given range, the stability of the mosquito repellent becomes poor and delamination occurs.

Example 6

The degradation rate of anophelifuge in the mosquito repellent described in examples 1 to 4, comparative examples 1 to 3, and comparative example 6 was measured.

The measurement method is as follows:

(1) Testing the content of anophelifuge: dissolving the anophelifuge with ethanol, taking n-octadecane as an internal standard substance, and performing gas chromatography separation and determination on the content of anophelifuge in the anophelifuge by using an HP-5 capillary column and a hydrogen flame ionization detector.

(2) And (3) testing the degradation rate: testing the content of anophelifuge in the anophelifuge according to the detection method, placing the anophelifuge in a thermostat at (54 + -2) deg.C for 14 days, taking out, wiping off the bottle, and testing the content of anophelifuge again.

The degradation rate M of the anophelifuge is calculated according to the following formula:

m- -anophelifuge degradation rate

W _{Front side} - -anophelifuge content of pre-heat storage sample

W _{Rear end} - -repellent ester content of samples after thermal storage

The test results are shown in table 3.

TABLE 3

As can be seen from Table 3, the degradation rates of the anophelifuge in the mosquito repellent are all less than 10%, and the mosquito repellent meets the requirements of pesticide registration certificates. When the surfactant is higher than the range given by the invention, the degradation rate of the anophelifuge is more than 10%.

Example 7

The mosquito repellent effects of the mosquito repellents described in examples 1 to 4, comparative examples 1 to 3, and comparative example 6 were measured.

The test method comprises the following steps: GB/T13917.9-2009 indoor pesticide efficacy test and evaluation for sanitary insecticides for pesticide registration certificate part 9: repellent

The test method comprises the following steps:

attack force test:

300 test insects are put into the mosquito cage, the back of the hand of a tester is exposed to the skin of 40mm multiplied by 40mm, and the rest part is tightly shielded. The hands are put into the mosquito cage for 2min, the mosquitoes are closely observed to stop falling, the arms are shaken to drive the mosquitoes away before the mouth organ of the mosquitoes penetrates into the skin, and the condition is recorded as that 1 test insect stops falling. More than 30 test persons and test insects stopping at 2min are qualified in attack power, and the persons and the mosquitoes in the cages can be subjected to repellent tests.

Repelling test:

selecting 4 persons with qualified offensive power (half of man and woman, and should not drink wine, tea or coffee before and during the test, and should not use essence-containing product), drawing skin area of 50mm × 50mm on the back of each hand, and pressing one hand to 1.5 μ L/cm ² The dosage of the mosquito repellent is uniformly smeared, 40mm multiplied by 40mm of skin is exposed, the rest part is closely shielded, and the other hand is a blank control. When the mosquito repellent is applied for 2h, the hands are put into a mosquito cage filled with mosquitoes with qualified attack power for 2min, and whether the mosquitoes come and stop and suck blood is observed. And then testing every 1h, and judging that the mosquito repellent fails as long as 1 mosquito sucks blood in front. The effective protection time (h) of the mosquito repellent was recorded. The contrast hand firstly tests each time, the tested insects with qualified attack force can continue to test, and the tested insects with unqualified attack force need to be replaced with qualified tested insects for testing.

The efficacy calculation method comprises the following steps:

the effective protection time of the mosquito repellent on 4 subjects is added, and the average value is taken as the effective protection time of the mosquito repellent. And performing pesticide effect evaluation on the effective protection time of testers according to the mosquito repellent, wherein the effective protection time of the A level is more than or equal to 6.0h, and the effective protection time of the B level is more than or equal to 4.0h.

The test results are shown in table 4.

TABLE 4

As can be seen from Table 4, the mosquito repellent of the present invention has an indoor repelling time of more than 6h and an A-grade drug effect.

Example 8

The irritation of the mosquito repellent to human skin is examined.

The test method is as follows: the subjects were 30 persons in total, with an age of 24 to 59 years, with 10 men and 20 women. Selecting qualified spot test equipment, placing 0.02-0.025 ml of mosquito repellent into the spot test equipment by using a closed spot test method, externally applying hypoallergenic adhesive tape to the back of a testee, removing the testee after 24 hours, observing skin reactions after 0.5, 24 and 48 hours after removal, and recording results according to skin reaction grading standards in technical Specification for safety of cosmetics (2015 edition), wherein the results are shown in Table 5.

TABLE 5

As can be seen from Table 5, the mosquito repellent of the present invention has no skin irritation.

Example 9

The antipruritic effects of the mosquito repellents described in examples 1 to 4 and comparative examples 1 to 3 were tested.

The test method is as follows: the mice are randomly divided into 9 groups, the mosquito repellent is smeared on the right hind paw of the mice according to the dose of 10g/kg, and the blank control group is smeared with the same amount of distilled water. After 10min interval, 0.05mL of histamine phosphate solution with the histamine phosphate mass percent of 0.01% is dripped on the wound surface of each mouse, and then 0.05mL is dripped on each wound surface of each mouse in a concentration increasing manner with the histamine phosphate mass percent of 0.01%, 0.02%, 0.03% and 0.04% in sequence every 3min interval until the mouse returns to lick the right hind paw, and the total amount of histamine given when the mouse returns to lick the right hind paw is taken as an itching relieving threshold, and the test results are shown in Table 6.

TABLE 6

	Number of experimental animals	Threshold of itching relieving (μ g)
			Example 1	4	420.5
Example 2	4	408.7
			Example 3	4	415.3
Example 4	4	410.5
			Comparative example 1	4	401.3
Comparative example 2	4	390.1
			Comparative example 3	4	380.6
Blank control group	4	36.1

As can be seen from Table 6, the mosquito repellent of the present invention has a high threshold of itching relief and a good effect of itching relief.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The mosquito repellent is characterized by comprising the following components in parts by weight: 6-15 parts of anophelifuge, 0.01-3 parts of balsam seed oil, 1-10 parts of main surfactant, 3-20 parts of cosurfactant, 0.02-0.1 part of hydroxyphenylpropionamide benzoic acid, 0.3-1 part of preservative, 0.1-0.3pH value buffer and the balance of water.

2. The mosquito repellent according to claim 1, wherein the weight portion of the mosquito repellent ester is 6-10, and the weight portion of the balsam seed oil is 0.01-1.

3. The mosquito repellent according to claim 1, wherein the mosquito repellent comprises the following components in parts by weight: 7 parts of anophelifuge ester, 0.2 part of balsam seed oil, 3 parts of main surfactant, 10 parts of cosurfactant, 0.05 part of hydroxyphenylpropionamide benzoic acid, 0.6 part of preservative, 0.2pH value buffer and the balance of water.

4. A mosquito repellent according to claim 1 wherein said primary surfactant is one of sodium cocoamidopropyl PG-dimethyl ammonium chloride phosphate, sodium lauroyl oat amino acid, sodium lauroyl glutamate, sodium lauroyl sarcosinate, sodium cocoyl glutamate, and sodium lauroyl lysine.

5. A mosquito repellent according to claim 1, wherein the co-surfactant is at least one of propylene glycol, methyl propylene glycol, butylene glycol and pentylene glycol.

6. A mosquito repellent according to claim 1, wherein said preservative is at least one of p-hydroxyacetophenone, hexylene glycol, caprylyl glycol, and caprylyl hydroxamic acid.

7. A process for the preparation of a mosquito repellent according to any one of claims 1 to 6, comprising the steps of:

(1) Adding water and a pH buffering agent into a stirring pot, and adjusting the pH value of the solution to 6.5 to 7;