CN112889844B - Method for preventing and treating culex pipiens pallens by using beauveria bassiana - Google Patents

Method for preventing and treating culex pipiens pallens by using beauveria bassiana Download PDF

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CN112889844B
CN112889844B CN202110137440.1A CN202110137440A CN112889844B CN 112889844 B CN112889844 B CN 112889844B CN 202110137440 A CN202110137440 A CN 202110137440A CN 112889844 B CN112889844 B CN 112889844B
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beauveria bassiana
polylactic acid
scenedesmus
mixture
mosquito
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CN112889844A (en
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刘丽娟
张本光
公茂庆
王海防
程鹏
刘宏美
郭秀霞
赵玉强
王怀位
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SHANDONG INSTITUTE OF PARASITIC DISEASES
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Abstract

The invention discloses a method for preventing and treating culex pipiens pallens by utilizing beauveria bassiana, which comprises the following steps of: 1) cultivating beauveria bassiana to obtain a beauveria bassiana culture; 2) centrifuging the beauveria bassiana culture, collecting precipitate, mixing the beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and drying at 30-40 deg.C to obtain beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing the beauveria bassiana mixture, the polylactic acid and the urea with different contents to prepare the film spraying liquid containing the urea with different contents; 3) preparing a pellet core by using expandable polystyrene beads; spraying a film on the pellet core for multiple times to obtain mosquito prevention and control particles; 4) and (3) scattering the mosquito prevention and control particles into a water body needing mosquito prevention and control, so as to realize mosquito prevention and control. The invention has the functions of trapping and collecting mosquito larvae and killing mosquitoes.

Description

Method for preventing and treating culex pipiens pallens by using beauveria bassiana
Technical Field
The invention belongs to the field of mosquito prevention and control. More particularly, the invention relates to a method for preventing and controlling culex pipiens pallens by utilizing beauveria bassiana.
Background
Mosquitoes belong to the order Diptera, the family of Mosquidae, also known as mosquitoes. The mosquito bites the human body not only have the reactions of red swelling, pruritus and the like, but also can cause the spread of diseases such as malaria, dengue fever, filariasis, Zika virus disease, epidemic encephalitis B and the like, thereby seriously harming the health of the human body. The life history of mosquitoes can be divided into four stages, namely eggs, larvae, pupae and adults. The first 3 stages of mosquitoes all live in water, adults live on land, and female mosquitoes need to lay eggs in accumulated water, and mosquito eggs must be incubated in water. The mosquito imagoes have strong wind-driving capability, the moving range is greatly increased, and the difficulty of mosquito prevention and control is increased. Therefore, the difficulty of controlling the culex pipiens pallens can be reduced by utilizing the fact that the mosquitoes live in water in the first 3 periods and are limited by the range of motion.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
It is still another object of the present invention to provide a method for controlling culex pipiens pallens using beauveria bassiana, which can induce mosquitoes to lay eggs in a specific water area and induce hatched mosquito larvae to perform a directional movement, thereby enhancing the control effect of the mosquito larvae.
To achieve these objects and other advantages of the present invention, there is provided a method for controlling culex pipiens pallens using beauveria bassiana, comprising the steps of:
1) crushing 10g of kelp into 40-50 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers of fishes and shrimps into 80-100 meshes to obtain powder of fishes and shrimps, putting 50mL of kelp filtrate, 1g of powder of fishes and shrimps, 1g of algae extract, 1g of yeast extract powder and 2g of activated carbon powder into 200mL of distilled water, uniformly mixing, inoculating 3-5% of beauveria bassiana, and performing shake culture at 25 ℃ for 5-7 days to obtain a beauveria bassiana culture;
2) centrifuging the beauveria bassiana culture, collecting precipitate, mixing 30-50g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder uniformly, and drying at 30-40 deg.C to obtain beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 30-50g of beauveria bassiana mixture, 1g of urea and 500mL of polylactic acid mixture to obtain a first membrane spraying solution; uniformly mixing 30-50g of beauveria bassiana mixture, 0.6g of urea and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 30-50g of beauveria bassiana mixture, 2g of cold-soluble polyvinyl alcohol powder, 0.3g of urea and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; putting 50-70g of pellet cores into a fluidized bed, spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles;
4) and (3) sprinkling the mosquito prevention and control particles into a water body needing mosquito prevention and control, so as to realize prevention and control of culex pipiens pallens.
According to the film forming property of polylactic acid, Beauveria bassiana and nutrient substances required by growth of the Beauveria bassiana are coated on the surface of a pellet core to form mosquito prevention and control particles with floating and slow release properties, when the mosquito prevention and control particles are thrown into accumulated water, the surface layers of the mosquito prevention and control particles are degraded to release substances such as lactic acid, ammonia and algae fishy smell, so that Culex pipiens pallidum is promoted to lay eggs near the mosquito prevention and control particles and larvae are close to the mosquito prevention and control particles, the Beauveria bassiana in the mosquito prevention and control particles grows, and infection of the Culex pipiens pallidum larvae and ova is realized through air flow and water flow, so that prevention and control of the mosquitoes are realized. The ammonia generated by urea hydrolysis has the effect of promoting polylactic acid hydrolysis to generate lactic acid, and both the ammonia and the urea are attractants for mosquitoes, so that the polylactic acid has the film-forming embedding effect and also has the effect of hydrolyzing the lactic acid to enhance the mosquito induction function. According to the invention, the decomposition speed of polylactic acid is regulated and controlled by regulating and controlling the urea content in the first film spraying liquid, the second film spraying liquid and the third film spraying liquid, so that the mosquito prevention and control particles prepared by the method have stronger attractant smell released later, and the mosquito attraction range is enhanced.
Preferably, the preparation method of the algae extract comprises the steps of dissolving chlorella, anabaena, custard crescenta, scenedesmus falcate, chrysotium dunnii, myristolochia multocida, scenedesmus bellatus and scenedesmus smith in 75% ethanol solution, alternately freezing and thawing for 2-3 times, centrifuging, collecting supernatant, and carrying out freeze drying to obtain the algae extract. The larvae of the culex pipiens pallens eat algae and bacteria, and the algae extract is added, so that the mosquito prevention and control particles can give off the fishy smell of the algae, thereby attracting the culex pipiens pallens to lay eggs in the water area, inducing the larvae to move to the mosquito prevention and control particles, and enhancing the prevention and control effect of the culex pipiens pallens.
Preferably, the mass ratio of chlorella to anabaena, cushing crescentella, scytalidium falciparum, chrysotium pernicioides, muliferous orbicularis, scenedesmus belladonna and smith scenedesmus is 1:1:1:1:1:1, and the sum of the masses of chlorella to anabaena, cushing crescentella, scytalidium falciparum, chrysotium pernici, muliferous orbicularis, scenedesmus belladonna and smith scenedesmus is 20-30% of the mass of the ethanol solution.
Preferably, 0.3g of urea is added into 500mL of polylactic acid solution to obtain polylactic acid urea solution, the mosquito prevention and control particles are placed in a fluidized bed, the polylactic acid urea solution is sprayed, and drying is carried out to obtain the coated mosquito prevention and control particles. A layer of polylactic acid film is formed on the surface layer of the mosquito prevention and control particles, and the effect of preventing the mosquito prevention and control particles from becoming damp and being degraded and invalid is achieved.
Preferably, 30g of pellet cores are placed in a fluidized bed, 100mL of first membrane spraying liquid is sprayed, 100mL of second membrane spraying liquid is sprayed after the first membrane spraying liquid is dried, and 100mL of third membrane spraying liquid is sprayed after the second membrane spraying liquid is dried, so that the mosquito prevention and control particles are obtained. Controlling the integral density of the mosquito prevention and control particles to make the density less than 1g/cm by controlling the dosage of the first film spraying liquid, the second film spraying liquid and the third film spraying liquid3Thereby leading the water surface to float, and realizing the attraction of imagoes and larvae in water above the water surface.
Preferably, 30-50g of beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture are uniformly mixed to obtain a first membrane spraying solution; uniformly mixing 30-50g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; and uniformly mixing 30-50g of beauveria bassiana mixture, 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution. The algae extracts are added into the first membrane spraying liquid, the second membrane spraying liquid and the third membrane spraying liquid, so that the insect attracting effect of the mosquito prevention and control particles can be enhanced.
Preferably, the preparation method of the expandable polystyrene beads comprises the steps of mechanically removing dust, removing impurities, washing with water, drying and crushing the recovered waste polystyrene foam plastics to obtain waste polystyrene foam; mixing new polystyrene, waste polystyrene foam, far infrared ceramic powder and magnesium peroxide according to a mass ratio of 5:2:0.05:0.02 to obtain a premix; directly adding the premix into a supercritical fluid extrusion foaming device to perform two-stage melt extrusion foaming, wherein the temperature range of an extruder at the first stage is 160-170 ℃, the rotating speed of a screw is 50r/min, and the gas injection amount of carbon dioxide is 10g/100g of raw material; the temperature range of the second-stage extruder is 150-160 ℃, the temperature of the neck ring mold is 100 ℃, the rotating speed of the screw is 10r/min, and expandable polystyrene beads with the particle size of 1mm are obtained by underwater granulating and circulating cooling water at the temperature of 30 ℃. By adding the far infrared ceramic powder, the mosquito prevention and control particles radiate more infrared rays, so that the insect trapping effect is further enhanced.
The invention at least comprises the following beneficial effects:
firstly, the beauveria bassiana is cultivated by kelp filtrate, fish and shrimp powder and yeast extract powder to induce the beauveria bassiana to generate more conidia, so that the beauveria bassiana has stronger resistance to adverse environment.
Secondly, according to the film forming property of polylactic acid, Beauveria bassiana and nutrient substances required by growth of the Beauveria bassiana are coated on the surface of a pellet core of the pellet to form mosquito prevention and control particles with floating and slow release properties, when the mosquito prevention and control particles are thrown into accumulated water, the surface layers of the mosquito prevention and control particles are degraded to release substances such as lactic acid, ammonia and fishy smell of algae, so that mosquitoes are promoted to lay eggs near the mosquito prevention and control particles and larvae are close to the mosquito prevention and control particles, the Beauveria bassiana in the mosquito prevention and control particles grows, and infection of the larvae, eggs and pupas of Culex pipiens pallens is realized through air flow and water flow, and therefore prevention and control of the mosquitoes are realized.
The ammonia generated by hydrolysis of the urea has the effect of promoting hydrolysis of the polylactic acid to generate lactic acid, and both the ammonia and the urea are attractants for mosquitoes, so that the polylactic acid has the film-forming embedding effect and also has the effect of hydrolyzing the lactic acid to enhance the mosquito induction function. According to the invention, the decomposition speed of polylactic acid is regulated and controlled by regulating and controlling the urea content in the first film spraying liquid, the second film spraying liquid and the third film spraying liquid, so that the mosquito prevention and control particles prepared by the method have stronger attractant smell released later, and the mosquito attraction range is enhanced.
By adding the algae extract, the mosquito prevention and control particles can emit fishy smell of algae, so that mosquitoes are attracted to lay eggs in the water area, larvae are induced to move to the mosquito prevention and control particles, and the mosquito prevention and control effect is enhanced.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
A method for preventing and treating Culex pipiens pallens by utilizing beauveria bassiana comprises the following steps:
1) crushing 10g of kelp into 40 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers into 80 meshes to obtain fish and shrimp powder, putting 50mL of kelp filtrate, 1g of fish and shrimp powder, 1g of algae extract, 1g of yeast extract powder and 2g of active carbon powder into 200mL of distilled water, uniformly mixing, inoculating 3-5% of beauveria bassiana, and performing shaking table culture at 25 ℃ for 5-7 days to obtain a beauveria bassiana culture;
2) centrifuging the beauveria bassiana culture, collecting precipitate, uniformly mixing 30g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and drying at 30 ℃ to obtain a beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 30g of beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture to obtain a first membrane spraying liquid; uniformly mixing 30g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 30g of beauveria bassiana mixture, 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; placing the pellet core into a fluidized bed and spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles;
4) and (3) scattering the mosquito prevention and control particles into a water body needing mosquito prevention and control, so as to realize mosquito prevention and control.
According to the film forming property of polylactic acid, Beauveria bassiana and nutrient substances required by growth of the Beauveria bassiana are coated on the surface of a pellet core to form mosquito prevention and control particles with floating and slow release properties, when the mosquito prevention and control particles are thrown into accumulated water, the surface layers of the mosquito prevention and control particles are degraded to release substances such as lactic acid, ammonia and algae fishy smell, so that mosquitoes are promoted to lay eggs near the mosquito prevention and control particles and larvae are close to the mosquito prevention and control particles, the Beauveria bassiana in the mosquito prevention and control particles grow, and infection of Culex pipiens pallens adults, Culex pipiens pallens eggs and larvae is realized through air flow and water flow, and accordingly, the prevention and control of Culex pipiens pallens is realized. The ammonia generated by urea hydrolysis has the effect of promoting polylactic acid hydrolysis to generate lactic acid, and both the ammonia and the urea are attractants for mosquitoes, so that the polylactic acid has the film-forming embedding effect and also has the effect of hydrolyzing the lactic acid to enhance the mosquito induction function. According to the invention, the decomposition speed of polylactic acid is regulated and controlled by regulating and controlling the urea content in the first film spraying liquid, the second film spraying liquid and the third film spraying liquid, so that the mosquito prevention and control particles prepared by the method have stronger attractant smell released later, and the mosquito attraction range is enhanced.
Example 2
A method for preventing and treating Culex pipiens pallens by utilizing beauveria bassiana comprises the following steps:
1) crushing 10g of kelp into 50 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers into 100 meshes to obtain powder of the fishes and the shrimps, putting 50mL of kelp filtrate, 1g of powder of the fishes and the shrimps, 1g of algae extract, 1g of yeast extract powder and 2g of active carbon powder into 200mL of distilled water, uniformly mixing, inoculating 3-5% of beauveria bassiana, and carrying out shake culture at 25 ℃ for 7 days to obtain a beauveria bassiana culture; the preparation method of the algae extract comprises the steps of dissolving chlorella, anabaena, custard crescent algae, scenedesmus falcate, chrysotile shield, myristolochia multocida, scenedesmus bellatus and scenedesmus smith in 75% ethanol solution, alternately freezing and thawing for 2 times, centrifuging, collecting supernatant, and freeze-drying to obtain the algae extract;
2) centrifuging the beauveria bassiana culture, collecting precipitate, uniformly mixing 40g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and drying at 40 ℃ to obtain a beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 50g of beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture to obtain a first membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; placing the pellet core into a fluidized bed and spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles;
4) and (3) scattering the mosquito prevention and control particles into a water body needing mosquito prevention and control, so as to realize mosquito prevention and control.
The larvae of the mosquitoes feed on algae and bacteria, and the algae extract is added to ensure that the mosquito prevention and control particles can emit fishy smell of the algae, so that the mosquitoes are attracted to the water area to lay eggs, the larvae are induced to move to the mosquito prevention and control particles, and the mosquito prevention and control effect is enhanced.
Example 3
A method for preventing and treating Culex pipiens pallens by utilizing beauveria bassiana comprises the following steps:
1) crushing 10g of kelp into 40 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers into 100 meshes to obtain powder of the fishes and the shrimps, putting 50mL of kelp filtrate, 1g of powder of the fishes and the shrimps, 1g of algae extract, 1g of yeast extract powder and 2g of active carbon powder into 200mL of distilled water, uniformly mixing, inoculating 3% of beauveria bassiana, and carrying out shake culture at 25 ℃ for 5 days to obtain a beauveria bassiana culture; the preparation method of the algae extract comprises the steps of dissolving chlorella, anabaena, custard crescent algae, scenedesmus falcate, chrysotile shield, myristolochia multocida, scenedesmus bellatus and scenedesmus smith in 75% ethanol solution, alternately freezing and thawing for 3 times, centrifuging, collecting supernatant, and freeze-drying to obtain the algae extract; the mass ratio of chlorella to anabaena, Coccomys kurotken, Scenedesmus falcatus, Scenedesmus dunnii, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 1:1:1:1:1:1, and the sum of the masses of chlorella, anabaena, Coccomys kutken, Scenedesmus falcatus, Scenedesmus jingensis, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 20% of the mass of the ethanol solution;
2) centrifuging the beauveria bassiana culture, collecting precipitate, uniformly mixing 50g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and drying at 30 ℃ to obtain a beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 40g of beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture to obtain a first membrane spraying liquid; uniformly mixing 40g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 40g of beauveria bassiana mixture, 2g of cold-soluble polyvinyl alcohol powder, 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; placing the pellet core into a fluidized bed and spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles;
4) and (3) scattering the mosquito prevention and control particles into a water body needing mosquito prevention and control, so as to realize mosquito prevention and control.
In this embodiment, the cold-soluble polyvinyl alcohol powder is added to the third membrane-spraying solution, so that the surface layer of the prepared mosquito prevention and control particles contains the cold-soluble polyvinyl alcohol which is easy to hydrolyze, when the mosquito prevention and control particles are put into water, the cold-soluble polyvinyl alcohol powder is hydrolyzed, a plurality of holes are formed on the surface layer of the particles, the specific surface area is increased, the growth of beauveria bassiana is facilitated, and conidia are easier to diffuse into the water body, so that the infection of the beauveria bassiana on mosquitoes is facilitated.
Example 4
A method for preventing and treating Culex pipiens pallens by utilizing beauveria bassiana comprises the following steps:
1) crushing 10g of kelp into 40 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers into 100 meshes to obtain powder of the fishes and the shrimps, putting 50mL of kelp filtrate, 1g of powder of the fishes and the shrimps, 1g of algae extract, 1g of yeast extract powder and 2g of active carbon powder into 200mL of distilled water, uniformly mixing, inoculating 3% of beauveria bassiana, and carrying out shake culture at 25 ℃ for 5 days to obtain a beauveria bassiana culture; the preparation method of the algae extract comprises the steps of dissolving chlorella, anabaena, custard crescent algae, scenedesmus falcate, chrysotile shield, myristolochia multocida, scenedesmus bellatus and Scenedesmus smithii in 75% ethanol solution, alternately freezing and thawing for 2-3 times, centrifuging, collecting supernate, and carrying out freeze drying to obtain the algae extract; the mass ratio of chlorella to anabaena, Coccomys kurotken, Scenedesmus falcatus, Scenedesmus dunnii, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 1:1:1:1:1:1, and the sum of the masses of chlorella, anabaena, Coccomys kutken, Scenedesmus falcatus, Scenedesmus kutken, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 20-30% of the mass of the ethanol solution;
2) centrifuging the beauveria bassiana culture, collecting precipitate, uniformly mixing 40g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and drying at 40 ℃ to obtain a beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 50g of beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture to obtain a first membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 2g of cold-soluble polyvinyl alcohol powder (PVA-1788), 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; putting 70g of pellet cores into a fluidized bed, spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles; adding 0.3g of urea into 500mL of polylactic acid solution to obtain polylactic acid urea solution, placing the mosquito prevention and control particles into a fluidized bed, spraying the polylactic acid urea solution, and drying to obtain coated mosquito prevention and control particles;
4) and (3) the coated mosquito prevention and control particles are scattered into a water body needing mosquito prevention and control, so that the prevention and control of the mosquitoes are realized.
A layer of polylactic acid film is formed on the surface layer of the mosquito prevention and control particles, and the effect of preventing the mosquito prevention and control particles from becoming damp and being degraded and invalid is achieved.
Example 5
A method for preventing and treating Culex pipiens pallens by utilizing beauveria bassiana comprises the following steps:
1) crushing 10g of kelp into 40 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers of fishes and shrimps into 90 meshes to obtain powder of fishes and shrimps, putting 50mL of kelp filtrate, 1g of powder of fishes and shrimps, 1g of algae extract, 1g of yeast extract powder and 2g of active carbon powder into 200mL of distilled water, uniformly mixing, inoculating 3% of beauveria bassiana, and carrying out shake culture at 25 ℃ for 7 days to obtain a beauveria bassiana culture; the preparation method of the algae extract comprises the steps of dissolving chlorella, anabaena, custard crescent algae, scenedesmus falcate, chrysotile shield, myristolochia multocida, scenedesmus bellatus and scenedesmus smith in 75% ethanol solution, alternately freezing and thawing for 3 times, centrifuging, collecting supernatant, and freeze-drying to obtain the algae extract; the mass ratio of chlorella to anabaena, Coccomys kurotken, Scenedesmus falcatus, Scenedesmus dunnii, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 1:1:1:1:1:1, and the sum of the masses of chlorella, anabaena, Coccomys kutken, Scenedesmus falcatus, Scenedesmus kutken, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 20-30% of the mass of the ethanol solution;
2) centrifuging the beauveria bassiana culture, collecting precipitate, uniformly mixing 50g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and drying at 40 ℃ to obtain a beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 50g of beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture to obtain a first membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 2g of cold-soluble polyvinyl alcohol powder (PVA-1788), 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; putting 65g of pellet cores into a fluidized bed, spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles; adding 0.3g of urea into 500mL of polylactic acid solution to obtain polylactic acid urea solution, placing the mosquito prevention and control particles into a fluidized bed, spraying the polylactic acid urea solution, and drying to obtain coated mosquito prevention and control particles; placing 30g of pellet cores in a fluidized bed, spraying 100mL of first membrane spraying liquid, spraying 100mL of second membrane spraying liquid after the first membrane spraying liquid is dried, and spraying 100mL of third membrane spraying liquid after the second membrane spraying liquid is dried to obtain the mosquito prevention and control particles;
4) and (3) the coated mosquito prevention and control particles are scattered into a water body needing mosquito prevention and control, so that the prevention and control of the mosquitoes are realized.
Controlling the integral density of the mosquito prevention and control particles to make the density less than 1g/cm by controlling the dosage of the first film spraying liquid, the second film spraying liquid and the third film spraying liquid3Thereby leading the water surface to float, and realizing the attraction of imagoes and larvae in water above the water surface. The density of the mosquito prevention and control particles prepared by the embodiment is 0.85g/cm3。
Example 6
A method for preventing and treating Culex pipiens pallens by utilizing beauveria bassiana comprises the following steps:
1) crushing 10g of kelp into 50 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers into 100 meshes to obtain powder of the fishes and the shrimps, putting 50mL of kelp filtrate, 1g of powder of the fishes and the shrimps, 1g of algae extract, 1g of yeast extract powder and 2g of active carbon powder into 200mL of distilled water, uniformly mixing, inoculating 5% of beauveria bassiana, and carrying out shake culture at 25 ℃ for 5 days to obtain a beauveria bassiana culture; the preparation method of the algae extract comprises the steps of dissolving chlorella, anabaena, custard crescent algae, scenedesmus falcate, chrysotile shield, myristolochia multocida, scenedesmus bellatus and scenedesmus smith in 75% ethanol solution, alternately freezing and thawing for 3 times, centrifuging, collecting supernatant, and freeze-drying to obtain the algae extract; the mass ratio of chlorella to anabaena, Coccomys kurotken, Scenedesmus falcatus, Scenedesmus dunnii, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 1:1:1:1:1:1, and the sum of the masses of chlorella, anabaena, Coccomys kutken, Scenedesmus falcatus, Scenedesmus jingensis, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 20% of the mass of the ethanol solution;
2) centrifuging the beauveria bassiana culture, collecting precipitate, uniformly mixing 50g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and drying at 40 ℃ to obtain a beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 50g of beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture to obtain a first membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 2g of cold-soluble polyvinyl alcohol powder (PVA-1788), 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; putting 60g of pellet cores into a fluidized bed, spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles; adding 0.3g of urea into 500mL of polylactic acid solution to obtain polylactic acid urea solution, placing the mosquito prevention and control particles into a fluidized bed, spraying the polylactic acid urea solution, and drying to obtain coated mosquito prevention and control particles; placing 30g of pellet cores in a fluidized bed, spraying 100mL of first membrane spraying liquid, spraying 100mL of second membrane spraying liquid after the first membrane spraying liquid is dried, and spraying 100mL of third membrane spraying liquid after the second membrane spraying liquid is dried to obtain the mosquito prevention and control particles;
4) and (3) the coated mosquito prevention and control particles are scattered into a water body needing mosquito prevention and control, so that the prevention and control of the mosquitoes are realized.
The algae extracts are added into the first membrane spraying liquid, the second membrane spraying liquid and the third membrane spraying liquid, so that the insect attracting effect of the mosquito prevention and control particles can be enhanced. The density of the coated mosquito prevention and control particles prepared by the embodiment is 0.90cm3/L。
Example 7
A method for preventing and treating Culex pipiens pallens by utilizing beauveria bassiana comprises the following steps:
1) crushing 10g of kelp into 40 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers into 80 meshes to obtain powder of the fishes and the shrimps, putting 50mL of kelp filtrate, 1g of powder of the fishes and the shrimps, 1g of algae extract, 1g of yeast extract powder and 2g of active carbon powder into 200mL of distilled water, uniformly mixing, inoculating 4% of beauveria bassiana, and carrying out shake culture at 25 ℃ for 6 days to obtain a beauveria bassiana culture; the preparation method of the algae extract comprises the steps of dissolving chlorella, anabaena, custard crescent algae, scenedesmus falcate, chrysotile shield, myristolochia multocida, scenedesmus bellatus and Scenedesmus smithii in 75% ethanol solution, alternately freezing and thawing for 2-3 times, centrifuging, collecting supernate, and carrying out freeze drying to obtain the algae extract; the mass ratio of chlorella to anabaena, Coccomys kurotken, Scenedesmus falcatus, Scenedesmus dunnii, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 1:1:1:1:1:1, and the sum of the masses of chlorella, anabaena, Coccomys kutken, Scenedesmus falcatus, Scenedesmus kutken, Mulberry haematococcus, Scenedesmus tridatus and Scenedesmus smithii is 20-30% of the mass of the ethanol solution;
2) centrifuging the beauveria bassiana culture, collecting precipitate, uniformly mixing 50g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and drying at 35 ℃ to obtain a beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 50g of beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture to obtain a first membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 50g of beauveria bassiana mixture, 2g of cold-soluble polyvinyl alcohol powder (PVA-1788), 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; putting 50g of pellet cores into a fluidized bed, spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles; adding 0.3g of urea into 500mL of polylactic acid solution to obtain polylactic acid urea solution, placing the mosquito prevention and control particles into a fluidized bed, spraying the polylactic acid urea solution, and drying to obtain coated mosquito prevention and control particles; placing 30g of pellet cores in a fluidized bed, spraying 100mL of first membrane spraying liquid, spraying 100mL of second membrane spraying liquid after the first membrane spraying liquid is dried, and spraying 100mL of third membrane spraying liquid after the second membrane spraying liquid is dried to obtain the mosquito prevention and control particles; the preparation method of the expandable polystyrene beads comprises the steps of mechanically removing dust from the recycled waste polystyrene foam plastics, removing impurities, washing with water, drying and crushing to obtain waste polystyrene foam; mixing new polystyrene, waste polystyrene foam, far infrared ceramic powder and magnesium peroxide according to a mass ratio of 5:2:0.05:0.02 to obtain a premix; directly adding the premix into a supercritical fluid extrusion foaming device to perform two-stage melt extrusion foaming, wherein the temperature range of an extruder at the first stage is 160-170 ℃, the rotating speed of a screw is 50r/min, and the gas injection amount of carbon dioxide is 10g/100g of raw material; the temperature range of the second-stage extruder is 150-160 ℃, the temperature of a neck ring mold is 100 ℃, the rotating speed of a screw is 10r/min, and expandable polystyrene beads with the particle size of 1mm are obtained by underwater granulating and circulating cooling water at the temperature of 30 ℃;
4) and (3) the coated mosquito prevention and control particles are scattered into a water body needing mosquito prevention and control, so that the prevention and control of the mosquitoes are realized.
By adding the far infrared ceramic powder, the mosquito prevention and control particles radiate more infrared rays, so that the insect trapping effect is further enhanced. By adding the magnesium peroxide, oxygen can be released to provide oxygen for the growth of beauveria bassiana. The density of the coated mosquito prevention and control particles prepared by the embodiment is 0.95cm3/L。
< test one > Effect of algae extract on egg-trapping of Culex pipiens pallens
20 culex pipiens pallens blood sucking mosquitoes are taken and placed into a mosquito net with the volume of 2.0m 1.8m, 2 spawning cups are placed in the mosquito net, 200mL of clean water is filled in the 2 spawning cups, 1 cup is not treated and serves as a control group, 0.1g of algae extract is added into the other cup and serves as an experimental group, each experimental group is taken out after 3 times of repeated spawning cups are placed in a mosquito cage for 24 hours, and the spawning number is recorded, and the results are shown in table 1.
TABLE 1 number of eggs laid
Average number of eggs (only)
Control group 15.7
Experimental group 4.3
As can be seen from the results of table 1, an average of 15.7 mosquitoes laid eggs in the water to which the algae extract was added, and only 4.3 mosquitoes laid eggs in the cups of the control group. It is demonstrated that the algae extract of the present invention has an effect of inducing mosquitoes to lay eggs in the water area to which they are added.
< test two > attraction test of algae extract to culex pipiens pallens larva
Taking 3 200mL cups, numbering A cup, B cup and C cup, then filling 3 cups with clear water, wherein the A cup is communicated with the B cup, the C cup is communicated with the B cup and is positioned on the opposite side of the A cup, placing 30 culex pipiens pallens larvae of 3 ages in the B cup, adding 0.1g of microalgae extract into the A cup in an experimental group, and not processing the C cup; in the control group, neither cup A nor cup C was treated, and the test was repeated 3 times, and the number of larvae in cup A, cup B and cup C was recorded, and the results are shown in Table 2.
TABLE 2 attraction effect of algae extract on Culex pipiens larvae
A cup (only) B cup (only) C cup (only)
Experimental group 18 7.7 4.3
Control group 8.7 12 9.3
As can be seen from the results in table 2, culex pipiens pallens larvae were significantly greater in the a cup than in the C cup in the experimental group after addition of the algae extract, while there was no significant difference in the larvae numbers in the a cup and the C cup without addition of the algae extract. The algae extract of the invention is proved to have the function of inducing the culex pipiens pallens larvae.
< third test > effect of attracting culex pipiens pallens larvae by far infrared ceramic powder
Taking 3 cups, numbering as cup A, cup B and cup C, then filling 3 cups with clear water, wherein the cup A is communicated with the cup B so that culex pipiens pallens larvae can freely move in the cup A and the cup B, the cup C is communicated with the cup B so that larvae can freely move in the cup C and the cup B, the cup A and the cup C are symmetrically arranged at two sides of the cup B, placing 30 larvae of 3-year-old larvae in the cup B, adding the mosquito prevention and control particles prepared in the embodiment 6 into the cup A in the experimental group 1, and keeping the cup B and the cup C from processing; in the experimental group 2, the mosquito control particles prepared in example 7 were added dropwise to cup a, and cup B and cup C were not treated; in the experimental group 3, the mosquito control particles prepared in example 7 were dropped into cup a, the mosquito control particles prepared in example 6 were dropped into cup C, and cup B was not treated; in the experimental group 4, the cup A, the cup B and the cup C are not treated, the addition amount of the mosquito prevention and control particles is 5% of the quality of the clear water, the experiment is repeated for 3 times, the number of mosquito larvae in the cup A, the cup B and the cup C is recorded, and the result is shown in table 3.
TABLE 3 attraction effect of prevention and control particles on Culex pipiens larvae of pale color
Figure BDA0002927555020000131
Figure BDA0002927555020000141
As can be seen from the results in table 3, the number of larvae in cup a in experimental group 1 is significantly greater than that in cup C, which indicates that the mosquito control particles prepared in example 6 have the function of trapping culex pipiens pallens larvae; the number of larvae in cup a in the experimental group 2 is significantly greater than that in cup C, which indicates that the mosquito control particles prepared in example 7 have trapping effect on larvae; the number of larvae in cup a in experimental group 2 was greater than that in cup a in example 1, and the number of larvae in cup a in experimental group 3 was greater than that in cup C, indicating that the mosquito control particles prepared in example 7 had better trapping effect on larvae than the mosquito control particles prepared in example 6.
< experiment four > mosquito prevention and control particles luring ovum to culex pipiens pallens
20 female blood-sucking culex pipiens pallens were placed in a 2.0m 1.8m mosquito net for each experiment, 2 spawning cups were placed in the mosquito net, 200mL of clear water was placed in the 2 spawning cups, the mosquito prevention and control particles prepared in examples 1 to 7 were added to cup a as an experimental group, cup B was a control group, the amount of mosquito prevention and control particles added was 5% of the mass of clear water, 3 replicates were set for each group of experiments, the spawning cups were placed in a mosquito cage for 24 hours, and the results of spawning number were recorded as shown in table 4.
TABLE 4 ovum inducing effect of different prevention and control granules
Figure BDA0002927555020000142
From the results in table 4, it can be seen that the mosquito control particles prepared by the present invention have the function of inducing mosquitoes to lay eggs in the water area.
Fifth experiment result of trapping and killing effect of mosquito prevention and control particles on culex pipiens pallens larvae
Trapping test for larvae
Taking 3 cups, numbering A cup, B cup and C cup, then filling 3 cups with clear water, wherein the A cup is communicated with the B cup to enable larvae to move freely in the A cup and the B cup, the C cup is communicated with the B cup to enable the larvae to move freely in the C cup and the B cup, the A cup and the C cup are symmetrically arranged on two sides of the B cup to form a test device, taking 8 groups of the test devices, placing 30 larvae of culex pipiens with 3 ages in the B cup, in the test group, dropwise adding the mosquito prevention and control particles prepared in the examples 1-7 into the A cup, and not processing the B cup and the C cup; in the control group, the cups A, B and C were not treated, the addition amount of the mosquito control particles was 5% of the clear water mass, the test was repeated 3 times, the number of larvae in the cups A, B and C was recorded for 24 hours, and the results are shown in Table 5.
TABLE 5 attraction effect of different prevention and control particles on culex pipiens pallens larvae
A cup (only) B cup (only) C cup (only)
Example 1 15 8.2 6.8
Example 2 17.5 7.9 4.6
Example 3 18.2 6.8 5
Example 4 16.4 7.5 6.9
Example 5 17.3 6.5 6.2
Example 6 17.1 6.9 6
Example 7 21.5 5.0 3.5
Control group 9.3 11.5 9.2
From the results in table 5, it can be seen that the mosquito control particles of the present invention have the effect of trapping culex pipiens pallens larvae.
Kill test for larvae
Taking 8 cups, adding 200mL of clear water into the cups, adding 30 larvae of culex pipiens pallens at the age of 3, adding the mosquito prevention and control particles prepared in the examples 1-7 into 7 of the cups respectively to serve as an experimental group, taking the other cup as a control group without treatment, wherein the adding amount of the mosquito prevention and control particles is 5% of the quality of the clear water, repeating the experiment for 3 times, and recording and counting the death rate of the larvae, wherein the results are shown in Table 6.
TABLE 6 mosquito larva mortality
Figure BDA0002927555020000161
From the results in table 6, it can be seen that the mosquito control particles prepared by the present invention have a good killing effect on culex pipiens pallens larvae.
< test six > prevention and control time test
The mosquito control particles prepared in examples 1 to 7 were immersed in water for 0, 15, 30, and 45 days, respectively, and then the mosquito control particles were taken out and placed in a cup containing 30 culex pipiens larvae in light color, the control group was not treated, the amount of the mosquito control particles added (before immersion) was 5% of the quality of the clear water, the test was repeated 3 times, and the number of deaths of 7d culex pipiens after treatment was recorded and counted, and the results are shown in table 7.
TABLE 7 mortality of larvae by different soaking time
Figure BDA0002927555020000162
From the results in table 7, it can be seen that the mosquito control particles prepared by the present invention have good killing effect on culex pipiens pallens larvae after being soaked in water for 45 days.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the uses set forth in the specification and examples. It can be applied to all kinds of fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. The invention is therefore not to be limited to the specific details described herein, without departing from the general concept as defined by the appended claims and their equivalents.

Claims (7)

1. A method for preventing and treating Culex pipiens pallens by utilizing beauveria bassiana is characterized by comprising the following steps of:
1) crushing 10g of kelp into 40-50 meshes, adding 100mL of water, alternately freezing and thawing for 2 times, extracting in 90 ℃ water for 6 hours, and performing vacuum filtration to obtain kelp filtrate; drying leftovers of fishes and shrimps, crushing the leftovers of fishes and shrimps into 80-100 meshes to obtain powder of fishes and shrimps, putting 50mL of kelp filtrate, 1g of powder of fishes and shrimps, 1g of algae extract, 1g of yeast extract powder and 2g of activated carbon powder into 200mL of distilled water, uniformly mixing, inoculating 3-5% of beauveria bassiana, and performing shake culture at 25 ℃ for 5-7 days to obtain a beauveria bassiana culture;
2) centrifuging the beauveria bassiana culture, collecting precipitate, mixing 30-50g of beauveria bassiana precipitate with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder uniformly, and drying at 30-40 deg.C to obtain beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing 30-50g of beauveria bassiana mixture, 1g of urea and 500mL of polylactic acid mixture to obtain a first membrane spraying solution; uniformly mixing 30-50g of beauveria bassiana mixture, 0.6g of urea and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; uniformly mixing 30-50g of beauveria bassiana mixture, 2g of cold-soluble polyvinyl alcohol powder, 0.3g of urea and 500mL of polylactic acid mixture to obtain a third membrane spraying solution;
3) placing expandable polystyrene beads into an intermittent pre-foaming machine, heating and softening the expandable polystyrene beads by steam, adjusting the pre-foaming temperature, the steam pressure and the feeding amount to ensure that the volume of volatile particles of a foaming agent is slowly expanded by 25 times, then enabling the expanded beads to freely fall into a curing bin made of mesh-shaped antistatic gauze for curing for 8 hours, entering a fluidized bed for drying, and screening powder to obtain pellet cores with the particle size of 1000-1200 um; putting 50-70g of pellet cores into a fluidized bed, spraying the first film spraying liquid, continuously spraying the second film spraying liquid after the first film spraying liquid is dried, continuously spraying the third film spraying liquid after the second film spraying liquid is dried, and drying the third film spraying liquid to obtain mosquito prevention and control particles;
4) and (3) sprinkling the mosquito prevention and control particles into a water body needing mosquito prevention and control, so as to realize prevention and control of culex pipiens pallens.
2. The method according to claim 1, wherein the algae extract is prepared by dissolving chlorella, anabaena, cudweed, scenedesmus falcatus, chrysotium brevifilis, sargassum morifolium, sargassum papyrii, sargassum papulosum morifolium, scenedesmus belladonna, and scenedesmus smithii in 75% ethanol solution, alternately freezing and thawing for 2-3 times, centrifuging, collecting supernatant, and freeze-drying to obtain the algae extract.
3. The method for controlling Culex pipiens pallens by using beauveria bassiana as claimed in claim 2, wherein the mass ratio of Chlorella, Anabaena, Cushing crescent moon, Scenedesmus falcatus, Chrysophytum esculentum, Morus alba, Scenedesmus protruiformis and Scenedesmus smith is 1:1:1:1:1:1:1:1, and the sum of the masses of Chlorella, Anabaena, Cushing crescent moon, Scenedesmus falcatus, Chrysophytum esculentum, Morus alba, Scenedesmus protruiformis and Scenedesmus smith is 20-30% of the mass of the ethanol solution.
4. The method for controlling culex pipiens pallens using beauveria bassiana as claimed in claim 1, wherein 0.3g of urea is added to 500mL of polylactic acid solution to obtain polylactic acid urea solution, the mosquito control particles are placed in a fluidized bed, the polylactic acid urea solution is sprayed, and the drying is performed to obtain the coated mosquito control particles.
5. The method for controlling culex pipiens pallens by using beauveria bassiana as claimed in claim 1, wherein the mosquito control particles are obtained by placing 30g pellet cores in a fluidized bed, spraying 100mL of the first film spraying liquid, spraying 100mL of the second film spraying liquid after the first film spraying liquid is dried, and spraying 100mL of the third film spraying liquid after the second film spraying liquid is dried.
6. The method for controlling Culex pipiens pallens by using Beauveria bassiana according to claim 1, wherein 30-50g of Beauveria bassiana mixture, 1g of urea, 1.5g of algae extract and 500mL of polylactic acid mixture are mixed uniformly to obtain a first membrane spraying solution; uniformly mixing 30-50g of beauveria bassiana mixture, 0.6g of urea, 1g of algae extract and 500mL of polylactic acid mixture to obtain a second membrane spraying solution; and uniformly mixing 30-50g of beauveria bassiana mixture, 0.3g of urea, 0.5g of algae extract and 500mL of polylactic acid mixture to obtain a third membrane spraying solution.
7. The method for controlling culex pipiens pallens by using beauveria bassiana according to claim 1, wherein the expandable polystyrene beads are prepared by subjecting the recovered waste polystyrene foam to mechanical dust removal, impurity removal, water washing, drying and crushing to obtain waste polystyrene foam; mixing new polystyrene, waste polystyrene foam, far infrared ceramic powder and magnesium peroxide according to a mass ratio of 5:2:0.05:0.02 to obtain a premix; directly adding the premix into a supercritical fluid extrusion foaming device to perform two-stage melt extrusion foaming, wherein the temperature range of an extruder at the first stage is 160-170 ℃, the rotating speed of a screw is 50r/min, and the gas injection amount of carbon dioxide is 10g/100g of raw material; the temperature range of the second-stage extruder is 150-160 ℃, the temperature of the neck ring mold is 100 ℃, the rotating speed of the screw is 10r/min, and expandable polystyrene beads with the particle size of 1mm are obtained by underwater granulating and circulating cooling water at the temperature of 30 ℃.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101027978A (en) * 2007-03-27 2007-09-05 中国科学院等离子体物理研究所 Floating microcapsule carrier under friendly environment and its production
CN101288647A (en) * 2007-04-18 2008-10-22 王雷波 Novel medicinal micropill core and preparation method thereof
CN110122442A (en) * 2019-05-06 2019-08-16 泰山医学院 A kind of mosquito control method suitable for pig farm

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100399892C (en) * 2004-07-14 2008-07-09 范小林 Water surface floated sustained-release medicament for preventing and treating blood fluke and method for preparing the same
CN1267479C (en) * 2004-12-28 2006-08-02 湖南大学 Nano particles of poly-lactic acid or polyethyllactide propyllactide for packing plant extract
CN100563640C (en) * 2006-10-20 2009-12-02 重庆大学 A kind of is the method for preparing microsphere of capsule material with the modified polylactic acid material

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101027978A (en) * 2007-03-27 2007-09-05 中国科学院等离子体物理研究所 Floating microcapsule carrier under friendly environment and its production
CN101288647A (en) * 2007-04-18 2008-10-22 王雷波 Novel medicinal micropill core and preparation method thereof
CN110122442A (en) * 2019-05-06 2019-08-16 泰山医学院 A kind of mosquito control method suitable for pig farm

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
球孢白僵菌对淡色库蚊的致病力研究;刘丽娟;《中国病原生物学杂志》;20121220;摘要，第848页第1栏，图1-3 *

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