CN112889710A - Treatment method for reducing death rate of postpartum sinocyclocheilus grahami - Google Patents
Treatment method for reducing death rate of postpartum sinocyclocheilus grahami Download PDFInfo
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- 241001275872 Sinocyclocheilus grahami Species 0.000 title claims abstract description 20
- 241000124815 Barbus barbus Species 0.000 claims abstract description 147
- 241000251468 Actinopterygii Species 0.000 claims abstract description 63
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 58
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- 238000002347 injection Methods 0.000 claims abstract description 44
- 239000007924 injection Substances 0.000 claims abstract description 44
- 238000012544 monitoring process Methods 0.000 claims abstract description 44
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 40
- 201000010099 disease Diseases 0.000 claims abstract description 39
- 239000003814 drug Substances 0.000 claims abstract description 27
- 208000024891 symptom Diseases 0.000 claims abstract description 22
- 229940079593 drug Drugs 0.000 claims abstract description 17
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- 238000003672 processing method Methods 0.000 claims abstract description 6
- 238000011534 incubation Methods 0.000 claims abstract description 4
- 230000008569 process Effects 0.000 claims description 23
- 229930182555 Penicillin Natural products 0.000 claims description 15
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 15
- 229940049954 penicillin Drugs 0.000 claims description 15
- LSQZJLSUYDQPKJ-NJBDSQKTSA-N amoxicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=C(O)C=C1 LSQZJLSUYDQPKJ-NJBDSQKTSA-N 0.000 claims description 12
- 229960003022 amoxicillin Drugs 0.000 claims description 12
- LSQZJLSUYDQPKJ-UHFFFAOYSA-N p-Hydroxyampicillin Natural products O=C1N2C(C(O)=O)C(C)(C)SC2C1NC(=O)C(N)C1=CC=C(O)C=C1 LSQZJLSUYDQPKJ-UHFFFAOYSA-N 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 239000003094 microcapsule Substances 0.000 claims description 11
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 10
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 10
- 235000019743 Choline chloride Nutrition 0.000 claims description 10
- 235000019733 Fish meal Nutrition 0.000 claims description 10
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 10
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- AYIRNRDRBQJXIF-NXEZZACHSA-N (-)-Florfenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CF)NC(=O)C(Cl)Cl)C=C1 AYIRNRDRBQJXIF-NXEZZACHSA-N 0.000 description 1
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- 206010027982 Morphoea Diseases 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/425—Thiazoles
- A61K31/429—Thiazoles condensed with heterocyclic ring systems
- A61K31/43—Compounds containing 4-thia-1-azabicyclo [3.2.0] heptane ring systems, i.e. compounds containing a ring system of the formula, e.g. penicillins, penems
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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Abstract
The invention relates to the technical field of fish culture, in particular to a processing method for reducing the death rate of postpartum sinocyclocheilus grahami. The invention provides a treatment method for reducing the death rate of postpartum sinocyclocheilus grahami, which comprises the following steps of: (1) preparing 2 breeding tanks including a monitoring tank and a breeding tank, soaking and disinfecting, and injecting water; (2) soaking and disinfecting the postpartum large-scale barbel fish; (3) classifying and feeding the large-scale barbel fish; (4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3); (5) and (3) checking and monitoring the growth state of the large-scale barbel fish with postpartum morbidity, transferring the large-scale barbel fish with postpartum morbidity symptom disappeared into an incubation trough for feeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the incubation trough, and repeating the steps (3) to (5) if the morbidity symptom appears. The method is simple and convenient, can reduce the disease rate and the death rate of the postpartum large-scale barbel fish, enables the postpartum large-scale barbel fish to quickly recover the physique and improves the immunity to pathogenic bacteria.
Description
Technical Field
The invention relates to the technical field of fish culture, in particular to a processing method for reducing the death rate of postpartum sinocyclocheilus grahami.
Background
Barbus capito belongs to Cyrtcheilus carpio, Barcheilus subfamily, and is also called fresh water pollock and fresh water pollock (in addition, seawater pollock) after entering China. Is a wide-temperature fish, and has the excellent characteristics of delicious meat, high growth speed, strong stress resistance, salt and alkali resistance and the like. The Amhese native to Uzbemustan is a famous large-scale economic fish, and is listed as a rare or endangered fish in China.
The stress resistance is strong, the salt and alkali resistance and other excellent properties are achieved, the culture technology is easy to master, the economic benefit is good, the water quality requirement is not high, and the method is a high-quality fish variety worthy of popularization. Compared with the prior art, the method has the advantages that the method is more cold-resistant than the prior art, and enriches the breeding varieties of the Chinese northern barbel.
The big scale ba cheilus grahami of perching habit perches in the well lower floor waters of pond water under artificial breeding condition, when the condition that the temperature is suitable, the environment is quiet does not have the disturbance under, also come up the surface of water occasionally, the cluster that opens during the time limit is around the pond around the trip, when it receives external environment disturbance, just sinks fast separately and flees. When the water temperature of the culture pond is reduced to below 15 ℃, the life style of the culture pond is mostly on the bottom layer of the pond water body, and the culture pond clusters at the deepest part of the pond bottom for overwintering. However, the induced spawning process needs to be performed manually under the condition of artificial breeding, the viscera of the parent fish are extruded by operations such as capturing, hastening spawning and extruding for many times in the artificial breeding process, and the phenomena of scale falling, skin injury, bleeding and the like occur, at the moment, the parent fish has large physical consumption, physical deficiency and serious injury, if the parent fish cannot be properly processed in time, bacterial and viral diseases such as fin rot, gill rot, mouth rot and tail rot are easily caused, and then saprolegniasis, white spot disease, bleeding disease and the like are caused until the death phenomenon of the parent fish occurs. In the actual production, the disease rate of the parent fish of the large-scale barbel is higher than 37%, the death rate is more than 10%, and the large-scale continuous breeding of the large-scale barbel is not facilitated.
Therefore, the problem that needs to be solved by the technical personnel in the field is how to provide a large-scale barbel fish postpartum treatment method which is simple to operate and can effectively reduce the disease rate and the death rate of the large-scale barbel fish.
Disclosure of Invention
In view of the above, the invention provides the large-scale barbel fish postpartum treatment method which is simple and convenient and can obviously reduce the disease rate and the death rate of the large-scale barbel fish, and the method can quickly restore the physique of the large-scale barbel fish and improve the immunity to pathogenic bacteria.
In order to achieve the purpose, the invention adopts the following technical scheme:
a treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks including a monitoring tank and a breeding tank, and injecting water;
(2) sterilizing the postpartum large-scale barbel fish body;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3);
(5) and (3) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 3-5 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears.
Preferably, the monitoring tank and the incubation tank in the step (1) are soaked and disinfected for 24-36h by 0.003% povidone iodine in advance, and then cleaned by clean water.
Preferably, the temperature of the water in the monitoring tank and the temperature of the water in the cultivating tank in the step (1) are both 20-25 ℃, and the flowing water of 0.5-0.7m/s in the tank is ensured.
Preferably, the medicine is veterinary penicillin or amoxicillin injection.
Preferably, the injection dosage of the medicament is as follows: amoxicillin injection: 1.5-1.8 micrograms/kg fish weight; penicillin for animals: 0.5-0.8 microgram/kg fish body weight.
Preferably, the postpartum large-scale barbel fish needs to be fed with a specific feed in the feeding process, and the feed comprises the following raw materials in parts by weight: 15-20 parts of fish meal, 1-2 parts of fish oil, 0.3-0.4 part of choline chloride, 0.1-0.2 part of fish multivitamin, 14-16 parts of bean pulp, 14-16 parts of earthworm powder and 6-8 parts of starch; and the sinking microcapsule feed is prepared by a microencapsulation process.
Preferably, in the step (2), the postpartum large-scale barbel fish is soaked and disinfected in a solution containing 1.4-1.8% of sodium chloride or 0.2-0.4% of formaldehyde for 35-45 min.
Preferably, the feed is fed in an amount of 3.5-4.5% by weight of the total fish body per day.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
1. the invention provides the large-scale barbel fish postpartum treatment method which is simple and convenient and can obviously reduce the disease rate and the death rate of the large-scale barbel fish, the method can lead the large-scale barbel fish to quickly recover the physique and improve the immunity to pathogenic bacteria;
2. the sodium chloride or formaldehyde solution can effectively sterilize the fish body of the postpartum large-scale barbel fish, and the veterinary penicillin or amoxicillin injection can effectively sterilize the fish body so as to reduce the disease rate or death rate of the postpartum large-scale barbel fish;
3. the large-scale barbel fish inhabits in the middle and lower water areas of the pond water body under the condition of artificial cultivation, belongs to omnivorous animals, and is mainly fed by low-lying animals or small fish and shrimps, so that the demand for protein is high. The special feed disclosed by the invention can provide sufficient nutrient components for the postpartum large-scale barbel fish, and the postpartum large-scale barbel fish can quickly pass the postpartum stage. The feed in the prior art is low in protein content and cannot meet the requirement of the large-scale barbel fish on protein after delivery, and the large-scale barbel fish needs to recover physical strength quickly, so that components capable of providing energy for the large-scale barbel fish quickly need to be added.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks of a monitoring tank and a breeding tank, soaking and disinfecting for 24 hours by using 0.003% povidone iodine in advance, then cleaning by using clear water, and then injecting water; the water temperatures in the monitoring tank and the cultivating tank are both 20 ℃, and the flowing water of 0.5m/s in the tanks is ensured;
(2) soaking and sterilizing the postpartum large-scale barbel fish in a sodium chloride solution containing 1.4 percent for 35 min;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3); the injection dose of the medicine is as follows: penicillin for animals: 0.5 microgram/kg fish weight;
(5) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 3 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears;
the method comprises the following steps that a specific feed needs to be fed to the postpartum large-scale barbel fish in the feeding process, and the feed comprises the following raw materials in parts by weight: 15 parts of fish meal, 1 part of fish oil, 0.3 part of choline chloride, 0.1 part of fish multivitamin, 14 parts of bean pulp, 14 parts of earthworm powder and 6 parts of starch; and preparing sinking microcapsule feed by a microencapsulation process; the feeding amount of the feed per day is 3.5% of the total weight of the fish body.
Example 2
A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks of a monitoring tank and a breeding tank, soaking and disinfecting for 36 hours by using 0.003% povidone iodine in advance, then cleaning by using clear water, and then injecting water; the water temperatures in the monitoring tank and the cultivating tank are both 25 ℃, and the flowing water of 0.7m/s in the tanks is ensured;
(2) soaking and sterilizing postpartum large-scale barbel fish in a 1.8% sodium chloride solution for 45 min;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3); the injection dose of the medicine is as follows: penicillin for animals: 0.8 microgram/kg fish weight;
(5) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 5 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears;
the method comprises the following steps that a specific feed needs to be fed to the postpartum large-scale barbel fish in the feeding process, and the feed comprises the following raw materials in parts by weight: 20 parts of fish meal, 2 parts of fish oil, 0.4 part of choline chloride, 0.2 part of fish multivitamin, 16 parts of bean pulp, 16 parts of earthworm powder and 8 parts of starch; and preparing sinking microcapsule feed by a microencapsulation process; the feeding amount of the feed per day is 4.5% of the total weight of the fish body.
Example 3
A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks of a monitoring tank and a breeding tank, soaking and disinfecting for 30 hours by using 0.003% povidone iodine in advance, then cleaning by using clear water, and then injecting water; the water temperature in the monitoring tank and the water temperature in the cultivating tank are both 22.5 ℃, and the flowing water of 0.6m/s in the tank is ensured;
(2) soaking and sterilizing the postpartum large-scale barbel fish in a 1.6% sodium chloride solution for 40 min;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3); the injection dose of the medicine is as follows: penicillin for animals: 0.65 micrograms/kg fish weight;
(5) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 4 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears;
the method comprises the following steps that a specific feed needs to be fed to the postpartum large-scale barbel fish in the feeding process, and the feed comprises the following raw materials in parts by weight: 17.5 parts of fish meal, 1.5 parts of fish oil, 0.35 part of choline chloride, 0.15 part of fish multivitamin, 15 parts of soybean meal, 15 parts of earthworm powder and 7 parts of starch; and preparing sinking microcapsule feed by a microencapsulation process; the feeding amount of the feed per day is 4% of the total weight of the fish body.
Example 4
A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks of a monitoring tank and a breeding tank, soaking and disinfecting for 30 hours by using 0.003% povidone iodine in advance, then cleaning by using clear water, and then injecting water; the water temperature in the monitoring tank and the water temperature in the cultivating tank are both 22.5 ℃, and the flowing water of 0.6m/s in the tank is ensured;
(2) soaking and sterilizing the postpartum large-scale barbel fish in a 0.3% formaldehyde solution for 40 min;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3); the injection dose of the medicine is as follows: amoxicillin injection: 1.65 micrograms/kg fish weight;
(5) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 4 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears;
the method comprises the following steps that a specific feed needs to be fed to the postpartum large-scale barbel fish in the feeding process, and the feed comprises the following raw materials in parts by weight: 17.5 parts of fish meal, 1.5 parts of fish oil, 0.35 part of choline chloride, 0.15 part of fish multivitamin, 15 parts of soybean meal, 15 parts of earthworm powder and 7 parts of starch; and preparing sinking microcapsule feed by a microencapsulation process; the feeding amount of the feed per day is 4% of the total weight of the fish body.
Example 5
A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks of a monitoring tank and a breeding tank, soaking and disinfecting for 30 hours by using 0.003% povidone iodine in advance, then cleaning by using clear water, and then injecting water; the water temperature in the monitoring tank and the water temperature in the cultivating tank are both 22.5 ℃, and the flowing water of 0.6m/s in the tank is ensured;
(2) soaking and sterilizing the postpartum large-scale barbel fish in a 0.2% formaldehyde solution for 40 min;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3); the injection dose of the medicine is as follows: amoxicillin injection: 1.5 micrograms per kilogram fish body weight;
(5) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 4 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears;
the method comprises the following steps that a specific feed needs to be fed to the postpartum large-scale barbel fish in the feeding process, and the feed comprises the following raw materials in parts by weight: 17.5 parts of fish meal, 1.5 parts of fish oil, 0.35 part of choline chloride, 0.15 part of fish multivitamin, 15 parts of soybean meal, 15 parts of earthworm powder and 7 parts of starch; and preparing sinking microcapsule feed by a microencapsulation process; the feeding amount of the feed per day is 4% of the total weight of the fish body.
Example 6
A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks of a monitoring tank and a breeding tank, soaking and disinfecting for 30 hours by using 0.003% povidone iodine in advance, then cleaning by using clear water, and then injecting water; the water temperature in the monitoring tank and the water temperature in the cultivating tank are both 22.5 ℃, and the flowing water of 0.6m/s in the tank is ensured;
(2) soaking and sterilizing the postpartum large-scale barbel fish in a 0.4% formaldehyde solution for 40 min;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3); the injection dose of the medicine is as follows: amoxicillin injection: 1.8 micrograms per kilogram fish body weight;
(5) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 4 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears;
the method comprises the following steps that a specific feed needs to be fed to the postpartum large-scale barbel fish in the feeding process, and the feed comprises the following raw materials in parts by weight: 17.5 parts of fish meal, 1.5 parts of fish oil, 0.35 part of choline chloride, 0.15 part of fish multivitamin, 15 parts of soybean meal, 15 parts of earthworm powder and 7 parts of starch; and preparing sinking microcapsule feed by a microencapsulation process; the feeding amount of the feed per day is 4% of the total weight of the fish body.
Example 7
A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks of a monitoring tank and a breeding tank, soaking and disinfecting for 30 hours by using 0.003% povidone iodine in advance, then cleaning by using clear water, and then injecting water; the water temperature in the monitoring tank and the water temperature in the cultivating tank are both 22.5 ℃, and the flowing water of 0.6m/s in the tank is ensured;
(2) soaking and sterilizing the postpartum large-scale barbel fish in a 1.6% sodium chloride solution for 40 min;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) injecting physiological saline into the postpartum sick large-scale barbel fish obtained in the step (3), wherein the injection amount is 0.65 micrograms/kg of fish weight;
(5) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 4 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears;
the method comprises the following steps that a specific feed needs to be fed to the postpartum large-scale barbel fish in the feeding process, and the feed comprises the following raw materials in parts by weight: 17.5 parts of fish meal, 1.5 parts of fish oil, 0.35 part of choline chloride, 0.15 part of fish multivitamin, 15 parts of soybean meal, 15 parts of earthworm powder and 7 parts of starch; and preparing sinking microcapsule feed by a microencapsulation process; the feeding amount of the feed per day is 4% of the total weight of the fish body.
Example 8
A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami comprises the following steps:
(1) preparing 2 breeding tanks of a monitoring tank and a breeding tank, soaking and disinfecting for 30 hours by using 0.003% povidone iodine in advance, then cleaning by using clear water, and then injecting water; the water temperature in the monitoring tank and the water temperature in the cultivating tank are both 22.5 ℃, and the flowing water of 0.6m/s in the tank is ensured;
(2) soaking postpartum large-scale barbel fish in sterile water for 40 min;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3); the injection dose of the medicine is as follows: penicillin for animals: 0.65 micrograms/kg fish weight;
(5) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 4 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears;
the method comprises the following steps that a specific feed needs to be fed to the postpartum large-scale barbel fish in the feeding process, and the feed comprises the following raw materials in parts by weight: 17.5 parts of fish meal, 1.5 parts of fish oil, 0.35 part of choline chloride, 0.15 part of fish multivitamin, 15 parts of soybean meal, 15 parts of earthworm powder and 7 parts of starch; and preparing sinking microcapsule feed by a microencapsulation process; the feeding amount of the feed per day is 4% of the total weight of the fish body.
Example 9
And (3) experimental verification: selecting 300 tails of female fishes and 300 tails of male fishes for artificial induced spawning, and 50 tails of female fishes and 50 tails of male fishes in each group.
Control group 1: a postpartum large-scale barbel fish without any treatment;
control group 2: the method of example 3 was followed except that the sterilization of the surface of the fish body in the step (2) was replaced with the sterilization method of the prior art (copper sulfate concentration of 8 mg/l, immersion washing for 20 minutes), and the other conditions were the same as in example 3;
control group 3: the method of example 3 is followed, but the drug injection is replaced by the method in the prior art (single injection per 1kg fish, 0.1-0.2 ml of 10% florfenicol injection), and other conditions are the same as in example 3;
example 7 is following the procedure of example 3 but replacing the drug injection with saline;
example 8 is a process according to example 3 but replacing the fish body surface sterilisation treatment in step (2) with a treatment with sterile water;
including example 3, 6 groups of postpartum large-scale barbel carps were recorded for disease rate and mortality, and the statistical results are shown in table 1.
The disease rate is equal to the number of the postpartum large-scale barbel fish with various diseases/the number of the postpartum large-scale barbel fish is multiplied by 100 percent;
the mortality rate is the number of the postpartum large-scale barbel fish died/the number of the postpartum large-scale barbel fish x 100%.
TABLE 1
| Example 3 | Example 7 | Example 8 | Control group 1 | Control group 2 | Control group 3 | |
| The disease rate% | 5 | 16 | 18 | 40 | 21 | 11 |
| The mortality rate is% | 2 | 9 | 10 | 18 | 13 | 8 |
The data in table 1 show that the disease rate and the mortality rate of the postpartum large-scale barbel can be obviously reduced by the treatment method, the disease rate is only 5%, the mortality rate is only 2%, and the mortality rate is obviously lower than that of other groups. The illness rate and death rate of the postpartum large-scale barbel fish without any treatment are the highest, and the comparison between the example 3 and the control group 3 shows that the medicine injection method is more suitable for the postpartum large-scale barbel fish; the comparison of example 3 with control 2 shows that the method of the present invention for sterilizing the surface of fish bodies is superior to the prior art.
Example 10
Experimental groups: a postpartum large barbel fish obtained according to the method of example 3;
control group: the treatment was carried out according to the method of example 3, except that the feed was replaced with conventional fish feed (rice bran 45%, bean cake 35%, silkworm chrysalis meal 10%, wheat middling 8%, bone meal 1.50%, salt 0.50%), and the resultant postpartum large-scale barbel fish was fed.
The average weight of the experimental group and the control group of the postpartum large-scale barbel fish is 2000g, the weight increase of the large-scale barbel fish is recorded through a single-factor experiment, the recovery condition of the large-scale barbel fish is observed, and the result is shown in table 2.
TABLE 2
As can be seen from table 2, the postpartum large-scale barbel fish fed by the feed of the invention not only has a fast postpartum recovery speed, but also has a significant weight gain effect on the postpartum large-scale barbel fish compared with the conventional fish feed, not only because the feed of the invention is a sinking microcapsule feed and is easy to feed the postpartum small-scale barbel fish with weak physique, but also the added feed components are more suitable for growth of the large-scale barbel fish.
Example 11
Experimental group 1: the postpartum large-scale barbel fish obtained was treated according to the method of example 3;
experimental group 2: a method for treating the Chinese barbel carps according to the example 3, wherein the penicillin for animals used in the example 3 is changed into amoxicillin injection (the injection amount is 1.65 micrograms/kilogram of fish body weight), and the obtained postpartum large barbel carps;
control group 1: the procedure of example 3 was followed except that the amount of veterinary penicillin injected in example 3 was changed to: 0.15 microgram/kg fish weight;
control group 2: the procedure of example 3 was followed except that the veterinary penicillin injection of example 3 was changed to amoxicillin injection and the injection amount was changed to: 1.15 micrograms/kg fish weight;
control group 3: the procedure of example 3 was followed except that the amount of veterinary penicillin injected in example 3 was changed to: 1.15 micrograms/kg fish weight;
control group 4: the procedure of example 3 was followed except that the veterinary penicillin injection of example 3 was changed to amoxicillin injection and the injection amount was changed to: 2.15 micrograms/kg fish weight;
and (3) carrying out single-factor experiments on 50 female fishes and 50 male fishes in each group, determining the disease rate and the death rate of the postpartum large-scale barbel fish, and observing the recovery condition of the postpartum large-scale barbel fish. The results are shown in Table 3.
TABLE 3
| The disease rate% | The mortality rate is% | Recovery situation | |
| Experimental group 1 | 5 | 3 | Good effect |
| Experimental group 2 | 5 | 2 | Good effect |
| Control group 1 | 21 | 13 | Is poor |
| Control group 2 | 25 | 15 | Is poor |
| Control group 3 | 4 | 17 | Difference (D) |
| Control group 4 | 4 | 16 | Difference (D) |
According to the data in table 3, it can be seen that the disease rate of the experimental groups 1 and 2 is significantly reduced relative to the control groups 1 and 2, and the mortality rate of the experimental groups 1 and 2 is significantly reduced relative to the control groups 1-4, which indicates that the disease rate and mortality rate of the postpartum large-scale barbel fish can be effectively reduced only by injecting the veterinary penicillin or amoxicillin injection solution in a proper proportion, so that the postpartum large-scale barbel fish can be better recovered, while the injection proportion of the drugs in the control groups 1 and 2 is too small, and a better bacteriostatic action cannot be achieved, while the disease rate of the control groups 3 and 4 is very low, but the injection proportion of the drugs is too large, and the mortality rate of the postpartum large-scale barbel fish is greatly improved due to drug reasons.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. A treatment method for reducing the death rate of postpartum sinocyclocheilus grahami is characterized by comprising the following steps:
(1) preparing 2 breeding tanks including a monitoring tank and a breeding tank, and injecting water;
(2) sterilizing the postpartum large-scale barbel fish body;
(3) classifying the large-scale barbel fish obtained in the step (2), and feeding the postpartum sick large-scale barbel fish into a monitoring trough; putting the postpartum healthy and disease-free and wound-free large-scale barbel fish into a cultivation tank for breeding;
(4) carrying out drug injection on the postpartum sick barbel carps obtained in the step (3);
(5) and (3) checking and monitoring the growth state of the postpartum diseased large-scale barbel fish in the cultivation tank every 3-5 days, transferring the large-scale barbel fish with the postpartum diseased symptom disappeared into the cultivation tank for breeding, paying attention to the health condition of the healthy, disease-free and wound-free large-scale barbel fish in the cultivation tank, and repeating the steps (3) - (5) if the diseased symptom appears.
2. The method for treating the postpartum sinocyclocheilus grahami mortality, according to claim 1, wherein the monitoring tank and the incubation tank in the step (1) are soaked and sterilized for 24-36 hours by using 0.003% povidone iodine in advance, and then are cleaned by using clear water.
3. The processing method for reducing the death rate of postpartum sinocyclocheilus grahami according to claim 1, wherein the water temperature in the monitoring tank and the water temperature in the cultivating tank in the step (1) are both 20-25 ℃, and the flowing water in the tank is ensured to be 0.5-0.7 m/s.
4. The processing method for reducing the mortality rate of postpartum sinocyclocheilus grahami according to claim 1, wherein the drug is selected from penicillin or amoxicillin injection for veterinary use.
5. The treatment method for reducing the mortality rate of postpartum sinocyclocheilus grahami according to claim 4, wherein the injection dosage of the drug is as follows: amoxicillin injection: 1.5-1.8 micrograms/kg fish weight; penicillin for animals: 0.5-0.8 microgram/kg fish body weight.
6. The processing method for reducing the mortality rate of the postpartum large-scale barbel according to claim 1, wherein the postpartum large-scale barbel needs to be fed with a specific feed in the feeding process, and the feed comprises the following raw materials in parts by weight: 15-20 parts of fish meal, 1-2 parts of fish oil, 0.3-0.4 part of choline chloride, 0.1-0.2 part of fish multivitamin, 14-16 parts of bean pulp, 14-16 parts of earthworm powder and 6-8 parts of starch; and the sinking microcapsule feed is prepared by a microencapsulation process.
7. The processing method for reducing the mortality rate of postpartum large-scale barbel according to claim 1, characterized in that in the step (2), the postpartum large-scale barbel fish is soaked and disinfected in a solution containing 1.4-1.8% of sodium chloride or 0.2-0.4% of formaldehyde for 35-45 min.
8. The method for reducing mortality of postpartum sinocyclocheilus grahami according to claim 6, wherein the daily feed is fed in an amount of 3.5-4.5% of the total weight of the fish.
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