CN112881127A - Sample slide preparation system in microscopic image analyzer and use method - Google Patents
Sample slide preparation system in microscopic image analyzer and use method Download PDFInfo
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- CN112881127A CN112881127A CN202110103574.1A CN202110103574A CN112881127A CN 112881127 A CN112881127 A CN 112881127A CN 202110103574 A CN202110103574 A CN 202110103574A CN 112881127 A CN112881127 A CN 112881127A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
Abstract
A system and a method for preparing a sample slide in a microscopic image analyzer are used for preparing the sample slide required by microscopic image analysis, the sample slide preparation system comprises an X-axis unit, a working shaft unit, a sucker unit, a power unit and a control unit, the X-axis unit comprises an X-axis stepping motor, a gear and an X-axis rack, the gear and the X-axis rack form gear-rack transmission, the working shaft unit is perpendicular to the X-axis unit and comprises a sampling shaft working module and a sucking disc shaft working module which are arranged in parallel, and can move up and down along the Z axis under the driving of respective motors, Tips is arranged at the lower end of the sampling shaft working module, the lower end of the sucker shaft working module is connected with a sucker unit, and the sucker unit is powered by the power unit to perform suction and discharge actions. The working shaft unit integrates the sampling shaft and the sucker shaft, and valuable space in the instrument is saved.
Description
Technical Field
The invention relates to the field of microscopic image analysis, in particular to a sample slide preparation system in a microscopic image analyzer and a using method thereof.
Background
When microscopic image analysis is performed, a microscope beam microscope and an electron microscope are required. The optical microscope has low cost and convenient use, and the magnification generally can meet the working requirement, so the optical microscope is mostly adopted when microscopic image analysis is carried out.
Optical microscopes require the preparation of a sample slide prior to the observation recording. The preparation of the sample slide requires first placing the sample on a slide and then covering the slide with a cover slip. During the operation, the operation of moving a cover glass, a slide glass, a liquid sample and the like needs to be carried out, and high precision is needed. Generally, the higher the precision, the more complex the system, and the larger the volume, the limited space in the instrument will be occupied, which is not favorable for the miniaturization of the instrument. Therefore, the size of the microscope image analyzer in the current market is large, and the cover glass moving module inside the microscope image analyzer in the current market is simple and crude, so that bubbles are often left in the sample slide, and the microscopic examination result is affected.
Disclosure of Invention
To solve the above problems, the present invention provides a system for preparing a sample slide in a lithography analyzer and a method for using the same. The system comprises an X-axis unit, a working shaft unit, a sucker unit and a power unit, wherein the working shaft unit simultaneously comprises a sampling shaft and a sucker shaft, so that precious space in an instrument is saved. The double-sucker design and the inclined plane design of the sucker unit enable the cover glass to be released at a certain angle, and air bubbles in the sample glass are effectively prevented.
The technical scheme of the invention is as follows:
a sample slide preparation system in a microscopic image analyzer is used for preparing a sample slide required by microscopic image analysis, the sample slide preparation system comprises an X-axis unit, a working shaft unit, a sucker unit, a power unit and a control unit, the X-axis unit comprises an X-axis stepping motor, a gear and an X-axis rack, the output end of the stepping motor is connected with the gear, the gear and the X-axis rack form gear rack transmission, the working shaft unit is arranged perpendicular to the X-axis unit, the working shaft unit comprises a sampling shaft working module and a sucking disc shaft working module which are arranged in parallel, and can move up and down along the Z axis under the driving of respective motors, Tips is arranged at the lower end of the sampling shaft working module, the lower end of the sucker shaft working module is connected with a sucker unit, and the sucker unit is powered by the power unit to perform suction and discharge actions. The working shaft unit integrates the sampling shaft and the sucker shaft, and valuable space in the instrument is saved.
Furthermore, the control unit is connected with the X-axis unit, the working shaft unit, the sucker unit and the power unit, receives data fed back by each working unit, sends an instruction to each working unit according to the fed-back data, and performs the next operation
As above a sample slide preparation system in apparent micro image analysis appearance, sample axle work module includes sample axle, Tips, miniature step motor, sample axle gear and sample axle rack, the sample axle sets up with the laminating of sample axle rack, the upper end of sample axle is provided with sample axle hose nipple, with the hose connection who connects plunger pump or sampling mechanism, provides the required power of sample of absorption by plunger pump or sampling mechanism, sample axle work module is by miniature step motor, sample axle gear and sample axle rack cooperation drive, and control setting is at the sample axle lower extreme Tips reciprocates the absorption and the drip of accomplishing the sample. The sample shaft is attached to the sampling shaft rack, so that the occupied space is reduced, the Z-axis of the sample shaft is driven to move without strong power, and the occupied space can be reduced as much as possible by using the micro stepping motor on the premise of meeting the power requirement.
As mentioned above sample slide preparation system in a apparent micro image analysis appearance, sucking disc axle work module includes sucking disc axle, brake step motor and sucking disc axle gear, the power pack is connected to the upper end of sucking disc axle, sucking disc axle one side is the rack structure to with sucking disc axle gear engagement, sample axle work module is by brake step motor and sucking disc axle gear drive sucking disc axle up-and-down motion.
Further, the sucking disc axle includes first sucking disc axle and second sucking disc axle, second sucking disc axle one side is fixed with sucking disc axle rack, first sucking disc axle and second sucking disc axle parallel arrangement, both ends are fixed through last locking piece and lower locking piece respectively, first sucking disc axle and second sucking disc axle top are provided with first sucking disc hose coupling and second sucking disc hose coupling respectively, first sucking disc hose coupling and second sucking disc hose coupling pass through the hose and are connected with power unit. The second sucking disc axle sets up with the laminating of sucking disc axle rack, has reduced the space and has occupy, chooses for use brake step motor drive sucking disc axle because sucking disc axle load is heavier, and brake step motor can effectively avoid the Z axle that the outage in the working process arouses to fall the equipment damage that causes.
Furthermore, the upper locking block is formed by symmetrically connecting two same M-shaped fixing blocks, and the shape and size of the lower locking block are the same as those of the upper locking block.
According to the sample slide preparation system in the microscopic image analyzer, the working shaft unit comprises the sampling sucker Z-shaft common adapter block, the common adapter block is provided with three vertically arranged through holes, the three through holes can respectively accommodate the sampling shaft, the first sucker shaft and the second sucker shaft to pass through, and two ends of the through holes through which the sampling shaft and the second sucker shaft pass through are provided with copper sleeves. The copper bush has good sliding property and self-lubricating property, and because the microscopic image analyzer belongs to precision machinery, lubricating means such as a lubricant and the like which easily cause part corrosion cannot be used, the copper bush is selected for lubrication, and in addition, the copper bush can play a role in limiting the working positions of the sampling shaft and the second sucker shaft.
Further, the inside of the through hole that the sampling shaft and the second sucker shaft pass is respectively connected with a first motor hole and a second motor hole which transversely penetrate, a sampling shaft gear is installed in the first motor hole, a sucker shaft gear is installed in the second motor hole, and the sampling shaft gear and the sucker shaft gear are respectively connected with the miniature stepping motor and the brake stepping motor. The miniature stepping motor and the brake stepping motor are respectively arranged on the miniature stepping motor connecting plate and the brake stepping motor connecting plate, and then the miniature stepping motor connecting plate and the brake stepping motor connecting plate are fixed on one side of the common transfer block.
Preferably, the lower end of the public adapter block is provided with a groove, a second limit switch is arranged in the groove, a photoelectric sensing piece is fixedly arranged on the lower locking block of the sucker shaft, a gap is formed in the second limit switch, and the photoelectric sensing piece can be inserted into the gap in the second limit switch. When the sucker shaft moves upwards to the limit under the driving of the brake stepping motor, the photoelectric sensing piece is inserted into a gap of the second limit switch, so that the second limit switch sends a signal to the control center, the brake stepping motor is stopped, and the sucker shaft is prevented from continuously moving to cause system damage.
According to the sample slide preparation system in the microscopic image analyzer, the X-axis unit comprises a connecting plate, a sliding block and a guide rail, the connecting plate is Z-shaped, the bottom end of the connecting plate is connected to the top end of the public switching block, the top end of the connecting plate is provided with the X-axis stepping motor with the output shaft facing downwards vertically, the sliding block and the guide rail are arranged in the middle of the connecting plate facing to the X-axis stepping motor, an X-axis rack is fixed on one face of the guide rail facing to the X-axis stepping motor, the sliding block is clamped on the guide rail and can slide along the transverse direction of the guide rail, an X-axis gear is arranged on the output shaft of the X-axis stepping. The connecting part of the sliding block and the guide rail is provided with a guide rail groove, the shape of the guide rail groove is matched with the shape of the cross section of the sliding block, and the sliding block cannot be separated from the guide rail groove after penetrating into the guide rail groove.
As above a sample slide preparation system in apparent micro image analysis appearance, the bottom of sucking disc axle is connected with the sucking disc unit, the sucking disc unit includes sucking disc subassembly and spacing seat, the sucking disc subassembly includes two sucking discs, spacing seat bottom is provided with and transversely runs through the decurrent sucking disc groove of opening, spacing seat bottom one side is provided with the inclined plane, the inclined plane is perpendicular with sucking disc subassembly array direction place face, the sucking disc subassembly sets up in the sucking disc groove, the sucking disc open end down, sucking disc tank bottom is provided with first limit switch, spacing seat top is provided with the sucking disc fixing base, be provided with the sucking disc hole that runs through in the middle of the sucking disc fixing base, sucking disc hole upper end is connected with the sample hub connection, and the lower extreme is connected with the sucking disc. The double-sucker design and the inclined plane design of the sucker unit enable the cover glass to be released at a certain angle, and air bubbles in the sample glass slide are effectively prevented.
As above sample slide preparation system in apparent micro image analysis appearance, power pack includes fixed plate, two vacuum diaphragm pumps and two three way solenoid valves are fixed to be set up on the fixed plate board, the fixed plate is fixed to be set up in public switching piece one side, two vacuum diaphragm pumps pass through the hose and are connected with two three way solenoid valves respectively, two three way solenoid valves pass through the hose and are connected with first sucking disc hose coupling and second sucking disc hose coupling respectively. The vacuum diaphragm pump is fixed on the fixing plate through the damping foot pad made of rubber, and the influence of motor vibration on the instrument is reduced.
The specific use method of the sample slide preparation system comprises the following steps:
1) the control unit sends out an instruction, and the sucker unit is driven by the working shaft unit and the X-axis unit to move to the position above the slide glass storage position;
2) the control unit sends out an instruction to drive the working shaft unit to descend, and the sucker unit is contacted with the glass slide and sucks the glass slide up under the action of the power unit;
3) the sucking disc unit for adsorbing the glass slide comes above the working area under the driving of the working shaft unit and the X-axis unit, and the control unit controls the sucking disc unit to move downwards to release the glass slide to the working area;
4) the control center sends out an instruction to control the sucker unit to move upwards, and then controls the working shaft unit and the X-axis unit to move above the sample;
5) the control center controls the sampling shaft working module to descend, and the descent is stopped when Tips contact the sample;
6) tips absorb a proper amount of samples;
7) the sampling shaft working module moves upwards, the Tips with samples are moved to the upper part of the glass slide under the driving of the working shaft unit and the X-axis unit, and the control center controls the sampling shaft working module to move downwards to the upper part of the glass slide and extrudes and releases the samples in the Tips onto the glass slide;
8) the control center controls the sampling shaft working module to ascend and descend, and the sucker unit is driven by the working shaft unit and the X-axis unit to move to the position above the cover glass storage position;
9) the control unit sends out an instruction to drive the working shaft unit to descend, and the sucker unit is contacted with the cover glass and sucks up the cover glass under the action of the power unit;
10) the sucker unit adsorbed with the cover glass is driven by the working shaft unit and the X-axis unit to move to the upper part of the working area, and the control unit controls the sucker unit to move downwards to release the cover glass onto a glass slide with a sample, and the sucker unit is in contact with the sample to complete the preparation of the sample glass slide.
The invention has the beneficial effects that:
1. according to the sample slide preparation system in the micro-image analyzer, the working shaft unit integrates the sampling shaft and the sucker shaft, so that precious space in the analyzer is saved.
2. This send sample slide preparation system in apparent lithography image analyzer, the copper sheathing has good slidability and self-lubricity, because apparent lithography image analyzer belongs to inseparable machinery, can't use lubricating means that lubricant etc. easily caused the part to corrode, consequently chooses for use the copper sheathing to lubricate for use, in addition, the copper sheathing can play the effect of injecing sample spindle and second sucking disc axle operating position.
3. This sample slide preparation system in the obvious lithography image analysis appearance, the design of the double suction cups of sucking disc unit and inclined plane make the cover glass can become certain angle release, effectively prevent to appear the bubble in the sample slide.
4. This send obvious sample slide preparation system in micro image analyzer, the sample shaft sets up with the laminating of sample shaft rack, has reduced the space and has occupy, and drive sample shaft Z axle removes and does not need too strong power, uses miniature step motor can reduce the space as far as and occupy under the prerequisite that satisfies the power demand.
5. This send obvious sample slide preparation system in the lithography image analyzer, second sucking disc axle sets up with sucking disc axle rack laminating, has reduced the space and has occupy, chooses for use brake step motor drive sucking disc axle because sucking disc axle load is heavier, and brake step motor can effectively avoid the equipment damage that the Z axle that the outage in the working process arouses falls and causes.
Drawings
The aspects and advantages of the present application will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention.
In the drawings:
FIG. 1 is a schematic perspective view of a specimen slide preparation system in a lithography analyzer according to example 1;
FIG. 2 is a schematic perspective view of another embodiment of a specimen slide preparation system in the lithography analyzer according to example 1;
FIG. 3 is a disassembled perspective view of the suction cup unit of embodiment 1;
FIG. 4 is a schematic perspective view of a working unit according to embodiment 1;
FIG. 5 is a sectional view of the working unit of example 1;
fig. 6 is a schematic perspective view of a common adapter block according to embodiment 1;
FIG. 7 is a side view of the X-axis unit and the common adapter block of embodiment 1;
FIG. 8 is a schematic perspective view of the X-axis unit of example 1;
FIG. 9 is a front view of the power unit of embodiment 1;
FIG. 10 is a schematic view showing suction of a cover glass by the suction cup unit of example 1;
FIG. 11 is a schematic view showing the position of the photoelectric sensor chip according to embodiment 2;
fig. 12 is a schematic perspective view of a common adapter block according to embodiment 2;
FIG. 13 is a schematic perspective view of a specimen slide preparation system in the lithography analyzer according to example 2;
the components represented by the reference numerals in the figures are:
1. x-axis unit, 11, X-axis stepping motor, 12, X-axis rack, 13, slider, 131, guide groove, 14, guide, 15, connection plate, 151, sampling double Z-axis guide connection plate, 152, sampling Z motor connection plate, 153, sampling rack motor connection plate, 16, X-axis gear, 2, working axis unit, 21, sampling axis working module, 211, sampling axis, 212, Tips, 213, micro stepping motor, 214, sampling axis gear, 215, sampling axis rack, 216, sampling axis hose connector, 22, suction axis working module, 221, suction axis, 2211, first suction axis, 2212, second suction axis, 2213, first suction axis hose, 2214, second suction hose connector, 222, brake hose connector stepping motor, 223, suction axis gear, 224, suction axis rack, 225, upper locking block, 226, lower locking block, 227, common adapter block, 2271, through hole, 2272. copper sheathing, 2273, first motor hole, 2274, second motor hole, 2275, second limit switch, 3, sucking disc unit, 31, sucking disc subassembly, 311, sucking disc, 32, spacing seat, 321, inclined plane, 322, sucking disc groove, 33, sucking disc fixing base, 331, sucking disc hole, 34, first limit switch, 35, photoelectric sensing piece, 4, power pack, 41, fixed plate, 42, vacuum diaphragm pump, 43, two three-way solenoid valve.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. It should be noted that these embodiments are provided so that this disclosure can be more completely understood and fully conveyed to those skilled in the art, and the present disclosure may be implemented in various forms without being limited to the embodiments set forth herein.
Example 1
Referring to fig. 1, a sample slide preparation system in a microscopic image analyzer is used for preparing a sample slide required by microscopic image analysis, the sample slide preparation system comprises an X-axis unit 1, a working axis unit 2, a sucker unit 3, a power unit 4 and a control unit, the X-axis unit 1 comprises an X-axis stepping motor 11, a gear and an X-axis rack 12, the output end of the stepping motor 11 is connected with the gear, the gear and the X-axis rack 12 form a rack-and-pinion transmission, the working axis unit 2 is arranged perpendicular to the X-axis unit 1, the working axis unit 2 comprises a sampling axis working module 21 and a sucker axis working module 22, the sampling axis working module 21 and the sucker axis working module 22 are arranged in parallel and can move up and down along the Z axis under the driving of respective motors, Tips212 is arranged at the lower end of the sampling axis working module 21, the sucker axis working module 22 is connected with the sucker unit 3 at the lower end, the sucking disc unit 3 is powered by the power unit 4 to perform sucking and releasing actions. The working shaft unit 2 integrates the sampling shaft working module 21 and the suction cup shaft working module 22, and valuable space in the instrument is saved.
Furthermore, the control unit is connected with the X-axis unit 1, the working axis unit 2, the sucker unit 3 and the power unit 4, receives data fed back by each working unit, and sends an instruction to each working unit according to the fed-back data to perform the next operation.
Referring to fig. 2, the bottom of sucking disc axle 221 is connected with sucking disc unit 3, sucking disc unit 3 includes sucking disc subassembly 31 and spacing seat 32, sucking disc subassembly 31 includes two sucking discs, spacing seat 32 bottom is provided with transversely runs through the decurrent sucking disc groove 322 of opening, spacing seat 32 bottom one side is provided with inclined plane 321, inclined plane 321 is perpendicular with sucking disc subassembly 31 range orientation place face, sucking disc subassembly 31 sets up in sucking disc groove 322, the sucking disc open end is down, sucking disc groove 322 bottom is provided with first limit switch 34, spacing seat 32 top is provided with sucking disc fixing base 33, be provided with the sucking disc hole 331 that runs through in the middle of sucking disc fixing base 33, sucking disc hole 331 upper end is connected with sample spindle 211, and the lower extreme is connected with the sucking disc. The double-sucker 311 design and the inclined plane 321 design of the sucker unit 3 enable the cover glass to be released at a certain angle, and air bubbles in the sample glass are effectively prevented.
Referring to fig. 3, the sampling shaft working module 21 includes a sampling shaft 211, Tips212, a micro stepping motor 213, a sampling shaft gear 214 and a sampling shaft rack 215, the sampling shaft 211 and the sampling shaft rack 215 are attached to each other, a sampling shaft hose joint 216 is disposed at the upper end of the sampling shaft 211 and connected to a hose connected to a plunger pump or a sampling mechanism, the plunger pump or the sampling mechanism provides power required for sucking a sample, and the sampling shaft working module 21 is driven by the micro stepping motor 213, the sampling shaft gear 214 and the sampling shaft rack 215 in a matching manner to control the Tips212 disposed at the lower end of the sampling shaft 211 to move up and down to complete sucking and dripping of the sample. The sample shaft 211 is attached to the sample shaft rack 215, so that the occupied space is reduced, the Z-axis movement of the sample shaft 211 is driven without strong power, and the occupied space can be reduced as much as possible by using the micro stepping motor 213 on the premise of meeting the power requirement.
Further, the sucker shaft working module 22 comprises a sucker shaft 221, a brake stepping motor 222 and a sucker shaft gear 223, the upper end of the sucker shaft 221 is connected with the power unit 4, one side of the sucker shaft 221 is of a rack structure and is meshed with the sucker shaft gear 223, and the sucker shaft working module 22 is driven by the brake stepping motor 222 and the sucker shaft gear 223 to move up and down.
Further, the sucker shaft 221 comprises a first sucker shaft 2211 and a second sucker shaft 2212, a sucker shaft rack 224 is fixed on one side of the second sucker shaft 2212, the first sucker shaft 2211 and the second sucker shaft 2212 are arranged in parallel, two ends of the first sucker shaft 2211 and the second sucker shaft 2212 are fixed through an upper locking block 225 and a lower locking block 226 respectively, a first sucker hose connector 2213 and a second sucker hose connector 2214 are arranged at the top ends of the first sucker shaft 2211 and the second sucker shaft 2212 respectively, and the first sucker hose connector 2213 and the second sucker hose connector 2214 are connected with the power unit 4 through hoses.
Further, the upper locking block 225 is formed by symmetrically connecting two identical M-shaped fixing blocks, and the shape and size of the lower locking block 226 are identical to those of the upper locking block. Second sucking disc axle 2212 sets up with the laminating of sucking disc axle rack 224, has reduced the space and has occupied, chooses for use brake step motor 222 drive sucking disc axle 221 because sucking disc axle 221 load is heavier, and brake step motor 222 can effectively avoid the equipment damage that the Z axle that the outage in the working process arouses falls and causes.
Referring to fig. 4-6, the working shaft unit 2 includes a sampling sucker Z-axis common adapter block 227, the common adapter block 227 is provided with three vertically arranged through holes 2271, the three through holes 2271 can accommodate the sampling shaft 211, the first sucker shaft 2211 and the second sucker shaft 2212 to pass through, and two ends of the through hole 2271 through which the sampling shaft 211 and the second sucker shaft 2212 pass through are provided with copper sleeves 2272. The copper sleeve 2272 has good sliding property and self-lubricating property, and since the microscopic image analyzer belongs to a precision machine, lubricating means such as a lubricant which is easy to cause part corrosion cannot be used, the copper sleeve 2272 is selected for lubrication, and in addition, the copper sleeve 2272 can play a role in limiting the working positions of the sampling shaft 211 and the second sucker shaft 2212.
Further, through hole 2271 inside that sample shaft 211 and second sucking disc axle 2212 passed is connected with first motor hole 2273 and the second motor hole 2274 that transversely runs through respectively, install sample shaft gear 214 in the first motor hole 2273, install sucking disc shaft gear 223 in the second motor hole 2274, sample shaft gear 214 and sucking disc shaft gear 223 are connected with miniature step motor 213 and brake step motor 222 respectively. The micro stepping motor 213 and the brake stepping motor 222 are respectively arranged on the connecting plate 15 of the micro stepping motor 213 and the connecting plate 15 of the brake stepping motor 222, and then the connecting plate 15 of the micro stepping motor 213 and the connecting plate 15 of the brake stepping motor 222 are fixed on one side of the common junction block 227.
Referring to fig. 7 and 8, the X-axis unit 1 includes a connecting plate 15, a sliding block 13 and a guide rail 14, the connecting plate 15 is Z-shaped, the connecting plate 15 includes a bottom L-shaped sampling double Z-axis guide rail connecting plate 151, a top planar sampling rack motor connecting plate 153, a middle inverted L-shaped sampling Z-axis motor connecting plate 152, the bottom sampling double Z-axis guide rail connecting plate 151 is connected to the top of a common adapter 227, the top sampling rack motor connecting plate 153 is provided with an X-axis stepping motor 11 with an output shaft vertically downward, the middle sampling Z-axis motor connecting plate 152 is provided with a sliding block 13 and a guide rail 14 facing the X-axis stepping motor 11, one side of the guide rail 14 facing the X-axis stepping motor 11 is fixed with an X-axis rack 12, the sliding block 13 is clamped on the guide rail 14 and can slide along the transverse direction of the guide rail 14, the output shaft of the X, the X-axis gear 16 is meshed with the X-axis rack 12. The connecting part of the slider 13 and the guide rail 14 is provided with a guide rail groove 131, the shape of the guide rail groove 131 is matched with the cross section shape of the guide rail 14, and the guide rail 14 cannot be separated from the guide rail groove 131 after penetrating into the guide rail groove 131.
Referring to fig. 9, the power unit 4 includes a fixing plate 41, two vacuum diaphragm pumps 42 and two-position three-way electromagnetic valves 43, the two vacuum diaphragm pumps 42 and the two-position three-way electromagnetic valves 43 are fixedly disposed on the fixing plate 41, the fixing plate 41 is fixedly disposed on one side of a common junction block 227, the two vacuum diaphragm pumps 42 are respectively connected to the two-position three-way electromagnetic valves 43 through hoses, and the two-position three-way electromagnetic valves 43 are respectively connected to a first suction cup hose connector 2213 and a second suction cup hose connector 2214 through hoses. The vacuum diaphragm pump 42 is fixed on the fixing plate 41 through a shock absorption foot pad made of rubber, so that the influence of motor vibration on the instrument is reduced.
The invention also provides a using method of the sample slide preparation system, which comprises the following steps:
1) the control unit sends out an instruction, the vacuum diaphragm pump 42 starts to work, the two-position three-way electromagnetic valve 43 is in a closed state, and the sucker unit 3 is driven by the working shaft unit 2 and the X-axis unit 1 to move to the position above the slide glass storage position.
2) The brake stepping motor 222 receives an instruction sent by the control unit, drives the sampling shaft 211 to descend, the suction cup 311 contacts with the slide, the first limit switch 34 is triggered, the two-position three-way electromagnetic valve 43 is opened, and the suction cup 311 generates suction force to suck up the slide.
3) The sucking disc unit 3 for adsorbing the glass slide comes above the working area under the driving of the working shaft unit 2 and the X-axis unit 1, the two-position three-way electromagnetic valve 43 is closed, and the control unit controls the sucking disc unit 3 to move downwards to release the glass slide to the working area;
4) the control unit sends out an instruction to control the sucker unit 3 to move upwards, the X-axis stepping motor 11 starts to rotate, and the working shaft unit 2 is driven by the X-axis unit 1 to move to the position above the sample.
5) The micro-stepper motor 213 drives the sample shaft 211 to descend, and the descent stops when the Tips212 contacts the sample.
6) The control unit sends a command to the plunger pump, the plunger pump starts to work to generate negative pressure, and the plunger pump stops working after the Tips212 sucks a certain amount of samples.
7) The sampling shaft working module 21 moves upwards, Tips212 which absorb samples are driven by the working shaft unit 2 and the X-axis unit 1 to move to the upper side of the glass slide, the control unit controls the sampling shaft working module 21 to move downwards to the upper side of the glass slide and sends signals to the plunger pump, the plunger pump starts to work to generate positive pressure, and the samples in the Tips212 are extruded and released onto the glass slide.
8) The control unit controls the sampling shaft working module 21 to ascend and descend, and the sucker unit 3 is driven by the working shaft unit 2 and the X-axis unit 1 to move to the position above the cover glass storage position.
9) The control unit sends out an instruction to drive the working shaft unit 2 to descend, the sucking disc 311 contacts with the cover glass, the first limit switch 34 is triggered, the two-position three-way electromagnetic valve 43 is opened, and the sucking disc 311 generates suction to suck up the cover glass.
10) The sucking disc unit 3 adsorbed with the cover glass is driven by the working shaft unit 2 and the X-axis unit 1 to come to the upper part of a working area, the two-position three-way electromagnetic valve 43 is closed, the control unit controls the sucking disc unit 3 to move downwards to release the cover glass onto a glass slide with a sample, see figure 10, the double sucking discs 311 and the inclined plane 321 of the sucking disc unit 3 are designed to enable the cover glass to fall at a certain angle when the cover glass is released, the angle between the cover glass and the glass slide is slowly reduced, and the cover glass and the glass slide are finally contacted with the sample to complete the preparation of the sample glass.
Example 2
Referring to fig. 11-13, the system additionally provides a solution of a safety protection device, a groove is provided at the lower end of the common adapter block 227, a second limit switch 2275 is provided in the groove, the lower locking block 226 of the sucker shaft 221 is fixedly provided with a photoelectric sensing piece 35, a gap is provided on the limit switch, and the photoelectric sensing piece 35 can be inserted into the gap on the limit switch. When the sucker shaft 221 moves upwards to the limit under the driving of the brake stepping motor 222, the photoelectric sensing piece 35 is inserted into the gap of the limit switch, so that the limit switch sends a signal to the controller, the brake stepping motor 222 is stopped, and the sucker shaft 221 is prevented from moving continuously to cause system damage.
Other features not mentioned in this embodiment can be the same as those in embodiment 1, and are not described again.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or additions or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. The utility model provides a sample slide preparation system in microscopic image analysis appearance for prepare the required sample slide of microscopic image analysis, its characterized in that, sample slide preparation system includes X axle unit (1), working shaft unit (2), sucking disc unit (3), power pack (4) and the control unit, X axle unit (1) includes X axle step motor (11), gear and X axle rack (12), step motor (11) output connection gear, the gear forms rack and pinion transmission with X axle rack (12), working shaft unit (2) perpendicular to X axle unit (1) sets up, working shaft unit (2) include sampling shaft work module (21), sucking disc axle work module (22), sampling shaft work module (21) and sucking disc axle work module (22) set up side by side, and can reciprocate along the Z axle under the drive of motor separately, the sample shaft working module (21) lower extreme is provided with Tips (212), sucking disc axle working module (22) lower extreme is connected with sucking disc unit (3), sucking disc unit (3) are provided power by power pack (4) and are inhaled the action of putting.
2. The specimen slide preparation system in a lithography analyzer according to claim 1, characterized in that the sampling shaft working module (21) comprises a sampling shaft (211), Tips (212), a micro stepping motor (213), a sampling shaft gear (214) and a sampling shaft rack (215), the sampling shaft (211) is attached to the sampling shaft rack (215), the upper end of the sampling shaft (211) is provided with a sampling shaft hose joint (216), is connected with a hose connected with a plunger pump or a sampling mechanism, the plunger pump or the sampling mechanism provides power required for sucking the sample, the sampling shaft working module (21) is driven by a micro stepping motor (213), a sampling shaft gear (214) and a sampling shaft rack (215) in a matching way, and controls Tips (212) arranged at the lower end of the sampling shaft (211) to move up and down to finish the suction and the dripping of a sample.
3. The system for preparing a sample slide in a lithography analyzer according to claim 1, wherein the chuck shaft working module (22) comprises a chuck shaft (221), a brake stepping motor (222) and a chuck shaft gear (223), the upper end of the chuck shaft (221) is connected with the power unit (4), one side of the chuck shaft (221) is of a rack structure and is engaged with the chuck shaft gear (223), and the chuck shaft (221) is driven to move up and down by the brake stepping motor (222) and the chuck shaft gear (223) by the sampling shaft working module (22).
4. A specimen slide preparation system in a microscopy image analyzer according to claim 3, wherein the sucker shaft (221) comprises a first sucker shaft (2211) and a second sucker shaft (2212), a sucker shaft rack (224) is fixed on one side of the second sucker shaft (2212), the first sucker shaft (2211) and the second sucker shaft (2212) are arranged in parallel, two ends of the first sucker shaft (2211) and the second sucker shaft (2212) are respectively fixed through an upper locking block (225) and a lower locking block (226), a first sucker hose connector (2213) and a second sucker hose connector (2214) are respectively arranged on the top ends of the first sucker shaft (2211) and the second sucker shaft (2212), and the first sucker hose connector (2213) and the second sucker hose connector (2214) are connected with the power unit (4) through hoses.
5. The system for preparing a specimen slide in a lithography analyzer according to claim 4, wherein said upper locking block (225) is formed by two identical M-shaped holding blocks symmetrically connected, and said lower locking block (226) is identical in shape and size to said upper locking block.
6. A sample slide preparation system in a microscopy image analyzer according to claim 4 or 5, wherein the working shaft unit (2) comprises a sampling sucker Z-axis common adapter block (227), the common adapter block (227) is provided with three vertically arranged through holes (2271), the three through holes (2271) can respectively accommodate a sampling shaft (211), a first sucker shaft (2211) and a second sucker shaft (2212) to pass through, and both ends of the through holes (2271) through which the sampling shaft (211) and the second sucker shaft (2212) pass through are provided with copper sleeves (2272).
7. The system for preparing a sample slide in a microscope image analyzer according to claim 6, wherein the through hole (2271) through which the sampling shaft (211) and the second sucker shaft (2212) pass is internally connected with a first motor hole (2273) and a second motor hole (2274) which transversely penetrate respectively, the first motor hole (2273) is internally provided with a sampling shaft gear (214), the second motor hole (2274) is internally provided with a sucker shaft gear (223), and the sampling shaft gear (214) and the sucker shaft gear (223) are respectively connected with the micro stepper motor (213) and the brake stepper motor (222).
8. The specimen slide preparation system in a lithography analyzer according to claim 1, characterized in that the X-axis unit (1) comprises a connecting plate (15), a slide block (13) and a guide rail (14), the connecting plate (15) is Z-shaped, the bottom end of the connecting plate is connected with the top end of a common adapter block (227), the top end of the connecting plate is provided with an X-axis stepping motor (11) with an output shaft vertically downward, the middle part of the connecting plate is provided with a sliding block (13) and a guide rail (14) facing to the X-axis stepping motor (11), an X-axis rack (12) is fixed on one surface of the guide rail (14) facing the X-axis stepping motor (11), the sliding block (13) is clamped on the guide rail (14), the X-axis stepping motor can slide along the transverse direction of the guide rail (14), an X-axis gear (16) is arranged on an output shaft of the X-axis stepping motor (11), and the X-axis gear (16) is meshed and connected with the X-axis rack (12).
9. The system for preparing a sample slide in a microscopic image analyzer according to claim 1, wherein a suction cup unit (3) is connected to the bottom end of the suction cup shaft (221), the suction cup unit (3) comprises a suction cup assembly (31) and a limiting seat (32), the suction cup assembly (31) comprises two suction cups (311), a suction cup groove (322) with a downward transverse through opening is formed in the bottom of the limiting seat (32), an inclined plane (321) is arranged on one side of the bottom of the limiting seat (32), the inclined plane (321) is perpendicular to the plane where the suction cup assembly (31) is arranged, the suction cup assembly (31) is arranged in the suction cup groove (322), the open end of the suction cup (311) faces downward, a first limiting switch (34) is arranged at the bottom of the suction cup groove (322), a suction cup fixing seat (33) is arranged above the limiting seat (32), the middle of the sucker fixing seat (33) is provided with a through sucker hole (331), the upper end of the sucker hole (331) is connected with the sampling shaft (211), and the lower end of the sucker hole (331) is connected with the sucker (311).
10. A method of using a specimen slide preparation system in a lithographic analyzer according to claim 1, comprising the steps of:
1) the control unit sends out an instruction, and the sucker unit (3) is driven by the working shaft unit (2) and the X-axis unit (1) to move to the position above the slide glass storage position;
2) the control unit sends out an instruction to drive the working shaft unit (2) to descend, the sucker unit (3) contacts with the glass slide and sucks the glass slide up under the action of the power unit (4);
3) the sucking disc unit (3) for adsorbing the glass slide is driven by the working shaft unit (2) and the X-axis unit (1) to move to the upper part of the working area, and the control unit controls the sucking disc unit (3) to move downwards to release the glass slide to the working area;
4) the control center sends out an instruction to control the sucker unit (3) to move upwards, and then controls the working shaft unit (2) and the X-axis unit (1) to move above the sample;
5) the control center controls the sampling shaft working module (21) to descend, and the descent stops when the Tips (212) contacts the sample;
6) tips (212) aspirate appropriate amount of sample;
7) the sampling shaft working module (21) moves upwards, the Tips (212) which absorb the samples move to the upper part of the glass slide under the driving of the working shaft unit (2) and the X-axis unit (1), and the control center controls the sampling shaft working module (21) to move downwards to the upper part of the glass slide and extrudes and releases the samples in the Tips (212) onto the glass slide;
8) the control center controls the sampling shaft working module (21) to ascend and descend, and the sucker unit (3) is driven by the working shaft unit (2) and the X-axis unit (1) to move to the position above the cover glass storage position;
9) the control unit sends out an instruction to drive the working shaft unit (2) to descend, the sucker unit (3) contacts the cover glass, and the cover glass is sucked up under the action of the power unit (4);
10) the sucker unit (3) adsorbed with the cover glass is driven by the working shaft unit (2) and the X-axis unit (1) to come to the upper part of the working area, and the control unit controls the sucker unit (3) to move downwards to release the cover glass onto a glass slide with a sample, and the cover glass is contacted with the sample to complete the preparation of the sample glass slide.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110103574.1A CN112881127B (en) | 2021-01-26 | 2021-01-26 | Sample slide preparation system in microscopic image analyzer and use method |
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| CN202110103574.1A CN112881127B (en) | 2021-01-26 | 2021-01-26 | Sample slide preparation system in microscopic image analyzer and use method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09325105A (en) * | 1996-06-04 | 1997-12-16 | Chiyoda Manufacturing Co Ltd | Method for taking out and fitting cover glass of microscope sample |
| US6302985B1 (en) * | 1996-12-18 | 2001-10-16 | Kabushiki Kaisha Tiyoda Seisakusho | Method of adhering cover glass and cover glass adhering device |
| JP2001290084A (en) * | 2000-04-10 | 2001-10-19 | Chiyoda Manufacturing Co Ltd | Cover glass sticking device for microscopic sample |
| US20160011221A1 (en) * | 2013-02-28 | 2016-01-14 | 3Dhistech Kft. | Automated Integrated Slide-Processing System |
| CN106537223A (en) * | 2014-07-09 | 2017-03-22 | 文塔纳医疗系统公司 | Automated coverslipper and methods of use |
| CN207751772U (en) * | 2018-01-12 | 2018-08-21 | 达科为（深圳）医疗设备有限公司 | A kind of mounting device |
-
2021
- 2021-01-26 CN CN202110103574.1A patent/CN112881127B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09325105A (en) * | 1996-06-04 | 1997-12-16 | Chiyoda Manufacturing Co Ltd | Method for taking out and fitting cover glass of microscope sample |
| US6302985B1 (en) * | 1996-12-18 | 2001-10-16 | Kabushiki Kaisha Tiyoda Seisakusho | Method of adhering cover glass and cover glass adhering device |
| JP2001290084A (en) * | 2000-04-10 | 2001-10-19 | Chiyoda Manufacturing Co Ltd | Cover glass sticking device for microscopic sample |
| US20160011221A1 (en) * | 2013-02-28 | 2016-01-14 | 3Dhistech Kft. | Automated Integrated Slide-Processing System |
| CN106537223A (en) * | 2014-07-09 | 2017-03-22 | 文塔纳医疗系统公司 | Automated coverslipper and methods of use |
| CN207751772U (en) * | 2018-01-12 | 2018-08-21 | 达科为（深圳）医疗设备有限公司 | A kind of mounting device |
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| Publication number | Publication date |
|---|---|
| CN112881127B (en) | 2022-12-20 |
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