CN112877278A - High-reproduction-rate embryo in-vitro production method - Google Patents
High-reproduction-rate embryo in-vitro production method Download PDFInfo
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Abstract
The invention discloses an in vitro production method of embryos with high reproduction rate, which comprises the following steps: a. collecting: the collected animal ovaries are ready for meeting laboratories; b. pretreatment: washing the oocyte with a maturation solution; c. fertilization: embryo transfer into receptor fluid; d. culturing: transferring the fertilized eggs into a cell culture box to start culture; the cell culture box in the step d comprises a main body, a fixed rod, a feed inlet, a discharge outlet, a separation plate, a through opening, a support plate, a movable plate, an air pressure bin, an air pressure plate, a pressing block, a buckle spring, a spiral groove, a ring groove, a butt joint groove and a rotating mechanism; the invention can implement observation of the development process of the embryo by culturing in the mode, thereby ensuring the breeding rate of the culture; when the embryo is placed in the main body, the gravity of the embryo drives the supporting plate to move downwards and rotate; the embryos are positioned below the septal plate and isolated from other supporting plates; so that the environment in the main body is not changed when the embryo is stored, thereby ensuring the activity of the embryo.
Description
Technical Field
The invention belongs to the technical field of in vitro production of animal embryos, and particularly relates to an in vitro production method of embryos with high reproduction rate.
Background
With the development of modern reproduction biotechnology and the gradual improvement of embryo in vitro production technology, animal in vitro production technology becomes an important way for accelerating variety breeding and propagation speed of excellent population; although live offspring have been obtained by embryo in vitro production technology, the in vitro embryos obtained by in vitro fertilization generally have development blocking phenomenon in the culture process, so that the blastocyst development rate of the in vitro embryos is far lower than that of the in vivo embryos; the current nutrient environment for in vitro culture of animal embryos is one of the key reasons causing the problems, for example, when the embryos are checked and placed, the environment temperature in the incubator is disturbed because the incubator needs to be opened; thereby affecting the development of the embryo and reducing the breeding efficiency.
Disclosure of Invention
The invention provides an in vitro embryo production method with high reproduction rate to overcome the defects of the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme: the method comprises the following steps:
a. collecting: the collected animal ovaries are ready for meeting laboratories;
b. pretreatment: washing the oocyte with mature liquid for 3 times, and culturing for 27-28 h;
c. fertilization: transferring the embryo into a receiving semen, wherein the receiving semen contains the oocytes treated in the step b;
d. culturing: transferring the fertilized eggs in the step c into a granular cell culture box, changing culture solution every 48h, culturing for 144-168h, and collecting embryos
The cell culture box in the step d comprises a main body, a fixed rod arranged in the main body, a feed inlet arranged at the top of the main body, a discharge outlet arranged on the side wall of the main body, a separation plate arranged in the main body, a through hole arranged on the separation plate, a plurality of support plates arranged on the fixed rod, a movable plate arranged on the support plates, and a moving device arranged on the fixed rod; the moving device comprises an air pressure bin arranged in the fixed rod, an air pressure plate arranged on the air pressure bin, a pressing block arranged on the air pressure plate, a buckle arranged on the pressing block, a buckle spring arranged on the buckle, a spiral groove arranged on the pressing block, a ring groove arranged on the pressing block, a butt joint groove arranged on the supporting plate, an air pressure spring arranged on the air pressure plate and a rotating mechanism arranged in the air pressure bin; opening the feed inlet, directly placing the culture dish filled with the embryos into the feed inlet, and driving the support plate to move downwards by the movable plate under the action of the gravity of the culture dish; further driving the pressing block to move downwards, and then moving downwards together with the air pressure plate; then, air pressure is generated in the air pressure bin to drive the rotating mechanism to rotate; then the supporting plate is driven to start rotating, and the supporting plate with the embryo culture dish is positioned below the partition plate; meanwhile, the supporting plate without the embryo culture dish rotates to the position right below the feeding hole to prepare for receiving materials.
The survival rate of the embryo is improved by culturing in the mode, and the breeding effect of the animal embryo can be effectively improved; the embryo culture dish can be directly placed on the movable plate through the arrangement of the feeding hole, so that the phenomenon that the temperature in the main body is changed due to direct contact with the inside of the main body when a test tube is placed is prevented, the constant temperature in the main body is ensured, and the activity of an embryo is further improved; the single discharging of the embryo culture dish is realized through the arrangement of the discharging port, so that the influence on the vitality of the embryo caused by the change of the stability of the inner part of the main body during the discharging process is prevented; the separation of the embryos from other support frames without embryo culture dishes is realized by the arrangement of the partition plates after the embryos are placed in the main body, so that the stability of test tube refrigeration is improved; the activity of the embryo is further improved; the single inlet and outlet of the supporting plate can be ensured through the arrangement of the through hole, and the storage stability of the embryo culture dish is improved; the embryo culture dish can be driven to move downwards when being stored through the arrangement of the supporting plate, so that the embryo culture dish is isolated from other supporting plates without the embryo culture dish, and the stability of a storage environment is ensured; the air pressure plate can generate air pressure in the air pressure bin according to the gravity of the embryo culture dish so as to drive the rotation mechanism to start, so that the linkage of embryo storage is improved, and the storage is more stable; on the other hand, the device can adapt to the low-temperature environment in the main body in an air pressure mode, so that the driving stability is improved, and the embryo storage activity is further improved; the pressing block is arranged to realize that the air pressure plate can be driven to move by independently moving the supporting plate; the stability of driving is further improved; the butt joint groove is arranged to enable the supporting plate to be embedded with the buckle when moving downwards, so that the pressing block is driven to move; the downward movement and the reset of the supporting plate can be separated from the pressing block, so that the downward movement stability of the supporting plate is improved; through the arrangement of the spiral groove, the supporting plate is rotated again when reset is carried out after unloading, and the storage stability of the embryo culture dish is improved.
And in the step a, the collected animal ovaries are brought back to the laboratory within 1-3h by using normal saline at the temperature of 30-38 ℃, and the oocytes are obtained by a suction method and placed in a preservation solution.
By the method, the ovarian activity of the animals which are just collected can be ensured, so that the effect of embryo reproduction is improved.
In the step c, the animal semen is treated by an improved BO liquid floating method; then the embryo is moved into the receptor fluid; after fertilization for 16-18h, the cumulus cells are dissolved for 1-2min by 0.2% hyaluronidase, and then are removed by Voatex vortex oscillation.
The method can effectively improve the activity of the fertilized embryo and further improve the embryo propagation effect.
The rotating mechanism is arranged on a partition plate on the fixed rod, a plurality of air outlet holes are formed in the partition plate, a rotating disc is arranged on the fixed rod, a circular ring frame is arranged on the rotating disc, a fan plate is arranged in the circular ring frame, a limiting assembly is arranged on the rotating disc, an air storage bin is arranged in the fixed rod, and a first one-way valve and a second one-way valve are arranged on the fixed rod; when the air pressure plate generates air pressure in the air pressure bin, the generated air pressure blows to the fan plate from the air outlet hole, and then the rotating disc is driven to rotate.
The change of the air pressure direction is realized through the arrangement of the air outlet holes, so that the fan plate is driven to rotate; the air pressure blown through the fan plate can be stored through the arrangement of the air storage bin, so that the stability of the air pressure in the fixed rod is ensured; can drive the rotation of backup pad through atmospheric pressure through the mechanism, can adapt to microthermal operational environment in the main part to improve the stability that the seminal fluid was stored, further improved the vitality of embryo.
The limiting assembly comprises a ratchet ring arranged on the fixed rod, a ratchet wheel arranged on the rotating disc, a limiting groove arranged in the fixed rod, a magnet arranged in the limiting groove, a butt-joint block arranged on the rotating disc and a magnetic wheel arranged on the butt-joint block; when the rotating disc starts to select, the rotating disc is rotated singly under the action of the ratchet ring and the ratchet gear; then, when the magnetic wheel rotates, the magnetic wheel moves along the inner wall of the fixed rod; the further magnetic wheel is butted with the limit groove after rotating, and is butted against the magnet when being connected with the butt joint groove.
The limit groove is arranged, so that the limit of the butt joint block can be realized after the butt joint block rotates by 45 degrees, the support plate can be always in butt joint with the feed port, and the storage stability of the embryo culture dish is improved; the alignment of the butt joint block and the limiting groove can be improved through the arrangement of the magnet and the magnetic wheel, so that the butt joint stability is improved; further improving the stability of embryo storage, thereby improving the effect of embryo development.
The movable plate comprises a phase caulking groove arranged on the supporting plate, a movable plate arranged in the phase caulking groove, a movable spring arranged on the movable plate, a clamping block arranged on the inner wall of the phase caulking groove, a clamping block spring arranged on the clamping block, a supporting spring arranged on the supporting plate and a positioning mechanism arranged on the movable plate; after the embryo culture dish is placed in the feeding port, firstly, the mouth of the culture dish is clamped on the moving plate, and the moving plate is driven to move downwards; then the supporting plate is positioned through a positioning mechanism; then when unloading is needed, the culture dish is directly taken out; when the clamping block is taken out, the clamping block is unlocked under the action of the clamping block spring.
The embryo culture dish can be stably erected on the manufacturing plate through the arrangement of the embedding grooves, so that the development stability of the embryo is improved; the embryo culture dish can be limited by the arrangement of the clamping block, the supporting plate is prevented from falling off when rotating, the stability of the embryo culture dish is improved, and the safety of embryo development is further improved; the device can stably support the embryo culture dish on the supporting plate, thereby improving the stability of embryo preservation; further improves the activity of the embryo, thereby leading the breeding effect to be better.
The positioning mechanism comprises a positioning groove arranged on the rotating disc, a positioning ring arranged in the positioning groove, a positioning block arranged at one end of the supporting plate, a positioning frame arranged on the positioning block, positioning teeth arranged in the positioning frame, a positioning spring arranged on the positioning teeth and a driving block arranged at one end of the moving plate; when the embryo culture dish is erected on the moving plate, the moving plate is driven to move downwards; then the driving block is abutted against the positioning block, and the driving block is abutted against the positioning frame at the moment, so that the positioning frame is separated from the positioning block; the further embryo culture dish drives the supporting plate to move downwards, and then the positioning block enters the positioning groove; at the moment, the positioning teeth are buckled with the positioning ring.
The positioning groove is arranged, so that the supporting plate can be limited, and the embryo culture dish is more stable to place; on the other hand, the supporting plate can not be shaken after being locked, so that the activity of the embryo is improved; the positioning ring can be abutted against the positioning teeth, so that the supporting plate is locked and the shaking of the supporting plate is placed; the stability of embryo storage is improved, and the embryo can not be bumped; the activity of the embryo is further improved; the arrangement of the mechanism can limit the position of the supporting plate through the gravity of the embryo culture dish, so that the storage stability of the embryo culture dish is improved; further improves the stability of embryo storage, thereby leading the embryo activity to be better; improving the effect of embryo in vitro production.
In conclusion, the invention has the following advantages: the invention can implement observation of the development process of the embryo by culturing in the mode, thereby ensuring the breeding rate of the culture; when the embryo is placed in the main body, the gravity of the embryo drives the supporting plate to move downwards and rotate; the embryos are positioned below the septal plate and isolated from other supporting plates; so that the environment in the main body is not changed when the embryo is stored, thereby ensuring the activity of the embryo.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a front view of the present invention.
Fig. 3 is a cross-sectional perspective view along a-a of fig. 2 of the present invention.
Fig. 4 is a cross-sectional view along a-a of fig. 2 of the present invention.
Fig. 5 is a cross-sectional view taken along B-B of fig. 2 of the present invention.
Fig. 6 is an exploded view of the present invention.
Fig. 7 is an exploded view of the rotating disk of the present invention.
Fig. 8 is a partial view of fig. 3 taken at a in accordance with the present invention.
Fig. 9 is a partial view of the invention at B in fig. 8.
Fig. 10 is a partial view of the invention at D in fig. 5.
Fig. 11 is an exploded view of the movable plate of the present invention.
Detailed Description
Example one
As shown in FIGS. 1-11, a method for producing embryos in vitro with high reproduction rate comprises the following steps:
a. collecting: bringing the collected animal ovaries back to a laboratory by using normal saline at 30 ℃ within 1h, and obtaining oocytes by using a suction method and placing the oocytes in a preservation solution;
b. pretreatment: washing the oocyte with a mature solution for 3 times, and culturing for 27 h;
c. fertilization: treating animal semen by modified BO liquid floating method; then the embryo is moved into the receptor fluid; after fertilization for 16h, dissolving cumulus cells for 1min by using 0.2% hyaluronidase, and then carrying out Voatex vortex oscillation to remove the cumulus cells;
d. culturing: transferring the fertilized eggs in the step c into a granular cell culture box, changing culture solution every 48 hours, and collecting embryos after culturing for 144 hours;
the cell culture box in the step d comprises a main body 1, a fixed rod 2, a feeding hole 3, a discharging hole 31, a separation plate 32, a through hole 33, a support plate 4, a movable plate 5 and a moving device 6; the moving device 6 comprises an air pressure bin 61, an air pressure plate 62, a pressing block 63, a buckle 64, a buckle spring 65, a spiral groove 66, a ring groove 67, a butt joint groove 68, an air pressure spring 69 and a rotating mechanism 7; the main body 1 is a culture box directly purchased from the market; the cross section of the fixed rod 2 is circular and is fixedly arranged in the main body 1; the feed inlet 3 is arranged at the top of the main body 1, and the feed inlet 3 is directly connected with a movable plate 5; the discharge hole 31 is formed in the side edge of the main body 1; the partition plate 32 is arranged in the main body 1; the through hole 33 is formed in the partition plate 32, and the size of the through hole is the same as that of the support plate 4; the number of the support plates 4 is 8, and the support plates are uniformly arranged along the circumferential direction of the main body 1; the cross section of the supporting plate 4 is fan-shaped; the movable plate 5 is arranged in the support plate 4; the moving device 6 is arranged in the fixed rod 2; the air pressure bin 61 is arranged in the fixing rod 2, and normal pressure air exists in the air pressure bin 61; the air pressure plate 62 can be embedded in the air pressure chamber 61 in a vertically moving manner; the pressing blocks 63 are provided with 8 pressing blocks which are uniformly arranged along the circumferential direction of the fixing rod 2; the pressing block 63 is movably embedded on the side wall of the fixed rod 2; the longitudinal section of the buckle 64 is triangular, and the buckle is movably embedded on the pressing block 63; the buckle spring 65 is connected with the buckle 64 and the pressing block 63; the butt joint groove 68 is formed in one end of the support plate 4 and penetrates through the left end and the right end of the support plate 4; the butt-joint groove 68 can be abutted against the buckle 64; the spiral groove 66 is arranged on the pressing block 63 and can be pressed against one end of the supporting plate 4; the annular groove 67 is formed in the bottom of the fixing rod 2 and the pressing block 63 and communicated with the spiral groove 66; the pneumatic spring 69 is arranged on the pneumatic plate 62 and is connected with the movable plate 5 and the pneumatic plate 62; the rotating mechanism 7 is arranged in the air pressure chamber 61.
As shown in fig. 7-8, the rotating mechanism 7 comprises a partition plate 71, an air outlet hole 72, a rotating disk 73, a circular ring frame 74, a fan plate 75, a limiting component 8, an air storage bin 76, a first one-way valve 77 and a second one-way valve 78; the partition plate 71 is arranged on the fixed rod 2 and is positioned in the air pressure bin 61; the plurality of air outlet holes 72 are uniformly arranged along the circumferential direction of the partition plate 71; the air outlet 72 is communicated with the baffle plate 71, and the axis of the air outlet 72 is inclined by 45 degrees with the surface of the circular ring frame 74; the rotating disc 73 is T-shaped in longitudinal section and is rotatably embedded on the fixed rod 2; the circular frame 75 is arranged on the rotating disc 73, and the longitudinal section of the circular frame 74 is U-shaped; the fan plates 75 are uniformly arranged along the circumferential direction of the circular ring frame 74; the limiting assembly 8 is arranged on the rotating disc 73; the gas storage bin 76 is arranged in the fixing rod 2; a plurality of first check valves 77 are arranged and evenly distributed along the circumferential direction of the gas storage bin 76; the first one-way valve 77 is directly opposite to the gas storage bin 76 and is positioned below the partition plate 71; a plurality of second check valves 78 are arranged and evenly distributed along the circumferential direction of the gas storage bin 76; the second check valve 78 is located directly opposite the pneumatic chamber 61 and above the diaphragm 71.
As shown in fig. 7 and 10, the limiting component 8 includes a ratchet ring 81, a ratchet wheel 82, a limiting groove 83, a magnet 84, a docking block 85, and a magnetic wheel 86; the ratchet ring 81 is arranged on the fixed rod 2; the ratchet gear 82 is arranged on the rotating disc 73 and is meshed with the ratchet ring 81; the cross section of each limiting groove 83 is arc-shaped, and the limiting grooves are 8 and are uniformly arranged along the circumferential direction of the fixed rod 2; the magnets 84 are arranged in each limiting groove 83; the butt joint block 85 is arranged on the rotating disc 73 and can abut against the inner wall of the fixed rod 2; the magnetic wheel 86 is rotatably embedded in the butt block 85 and can abut against the magnet 84.
As shown in fig. 11, the movable plate 5 includes a mating groove 51, a movable plate 52, a movable spring 53, a latch 54, a latch spring 55, a support spring 56, and a positioning mechanism 9; the cross section of the embedding groove 51 is U-shaped and is arranged on the supporting plate 4; the moving plate 52 can be embedded in the supporting plate 4 in an up-and-down moving way; one end of the moving plate 52 is semicircular in cross section and is positioned in the embedded groove 51; the moving spring 53 connects the moving plate 52 and the support plate 4; the fixture blocks 54 are movably embedded at two ends of the embedding groove 51, and the cross sections of the fixture blocks 54 are arc-shaped; the fixture spring 55 is connected with the fixture 54 and the support plate 4; the support spring 56 connects the support plate 4 and the rotating disk 73; the positioning mechanism 9 is disposed on the moving plate 52.
As shown in fig. 8-9, the positioning mechanism 9 includes a positioning groove 91, a positioning ring 92, a positioning block 93, a positioning frame 94, a positioning tooth 95, a positioning spring 96, and a driving block 97; the positioning groove 91 is arranged on the rotating disc 73; the positioning ring 92 is triangular in longitudinal section and is annularly arranged in the positioning groove 91; the positioning frame 94 is movably embedded in the positioning block 93, and the longitudinal section of the positioning frame 94 is trapezoidal; the positioning frame 94 is connected with the positioning block 93 by a spring; the positioning block 93 is fixedly arranged at one end of the supporting plate 4, and the longitudinal section of the positioning block is U-shaped; the positioning teeth 95 are movably embedded in the positioning frame 94, and the cross section is trapezoidal; the positioning spring 96 is connected with the positioning teeth 95 and the positioning frame 94; the driving block 97 is disposed on the moving plate 52 and can abut against the positioning frame 94.
The specific implementation process is as follows: opening the feed inlet, directly placing the culture dish filled with embryos into the feed inlet 3, and driving the support plate to move downwards by the movable plate 5 under the gravity of the culture dish; further driving the pressing block 63 to move downwards, and at the same time, the pressing block and the air pressure plate 62 move downwards; then, air pressure is generated in the air pressure bin 61 to drive the rotating mechanism to rotate; then the support plate 4 is driven to rotate, and the support plate 4 with the embryo culture dish is positioned below the partition plate 32; meanwhile, the supporting plate 4 without the embryo culture dish rotates to the position right below the feeding hole 3 to prepare for receiving materials.
Example two
An in vitro production method of embryos with high reproduction rate comprises the following steps:
a. collecting: bringing the collected animal ovaries back to a laboratory by using normal saline at 38 ℃ within 3h, and obtaining oocytes by using a suction method and placing the oocytes in a preservation solution;
b. pretreatment: washing the oocyte with a mature solution for 3 times, and culturing for 28 h;
c. fertilization: treating animal semen by modified BO liquid floating method; then the embryo is moved into the receptor fluid; after fertilization for 18h, dissolving cumulus cells for 2min by using 0.2% hyaluronidase, and then carrying out Voatex vortex oscillation to remove the cumulus cells;
d. culturing: transferring the fertilized eggs in the step c into a granular cell culture box, changing culture solution every 48 hours, and collecting embryos after culturing for 168 hours;
EXAMPLE III
An in vitro production method of embryos with high reproduction rate comprises the following steps:
a. collecting: bringing the collected animal ovaries back to a laboratory at 34 ℃ by using normal saline within 2h, and obtaining oocytes by using a suction method and placing the oocytes in a preservation solution;
b. pretreatment: washing the oocyte with mature liquid for 3 times, and culturing for 27.5 h;
c. fertilization: treating animal semen by modified BO liquid floating method; then the embryo is moved into the receptor fluid; after fertilization for 17h, dissolving cumulus cells for 1.5min by using 0.2% hyaluronidase, and then carrying out Voatex vortex oscillation to remove the cumulus cells;
d. culturing: transferring the fertilized eggs in the step c into a granular cell culture box, changing culture solution every 48 hours, and collecting embryos after culturing for 150 hours;
while there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (7)
1. An in vitro embryo production method with high reproduction rate is characterized in that: the method comprises the following steps:
a. collecting: the collected animal ovaries are ready for meeting laboratories;
b. pretreatment: washing the oocyte with mature liquid for 3 times, and culturing for 27-28 h;
c. fertilization: transferring the embryo into a receiving semen, wherein the receiving semen contains the oocytes treated in the step b;
d. culturing: transferring the fertilized eggs in the step c into a granular cell culture box, changing culture solution every 48 hours, and collecting embryos after culturing for 144-168 hours;
the cell culture box in the step d comprises a main body (1), a fixed rod (2) arranged in the main body (1), a feeding hole (3) arranged at the top of the main body (1), a discharging hole (31) arranged on the side wall of the main body (1), a partition plate (32) arranged in the main body (1), a through hole (33) arranged on the partition plate (32), a plurality of support plates (4) arranged on the fixed rod (2), a movable plate (5) arranged on the support plates (4), and a moving device (6) arranged on the fixed rod (2); the moving device (6) comprises an air pressure bin (61) arranged in the fixed rod (2), an air pressure plate (62) arranged on the air pressure bin (61), a pressing block (63) arranged on the air pressure plate (62), a buckle (64) arranged on the pressing block (63), a buckle spring (65) arranged on the buckle (64), a spiral groove (66) arranged on the pressing block (63), a ring groove (67) arranged on the pressing block (63), a butt joint groove (68) arranged on the support plate (4), an air pressure spring (69) arranged on the air pressure plate (62) and a rotating mechanism (7) arranged in the air pressure bin (61); the feed inlet is opened, the culture dish filled with embryos is directly placed into the feed inlet (3), and then the movable plate (5) drives the support plate to move downwards under the action of the gravity of the culture dish; further driving the pressing block (63) to move downwards, and then moving downwards together with the air pressure plate (62); then, air pressure is generated in the air pressure bin (61) to drive the rotating mechanism to rotate; then the supporting plate (4) is driven to rotate, and the supporting plate (4) with the embryo culture dish is positioned below the partition plate (32); meanwhile, the supporting plate (4) without the embryo culture dish rotates to the position right below the feeding hole (3) to prepare for receiving materials.
2. The method for producing embryos in vitro with high reproduction rate according to claim 1, wherein: and in the step a, the collected animal ovaries are brought back to the laboratory within 1-3h by using normal saline at the temperature of 30-38 ℃, and the oocytes are obtained by a suction method and placed in a preservation solution.
3. The method for producing embryos in vitro with high reproduction rate according to claim 1, wherein: in the step c, the animal semen is treated by an improved BO liquid floating method; then the embryo is moved into the receptor fluid; after fertilization for 16-18h, the cumulus cells are dissolved for 1-2min by 0.2% hyaluronidase, and then are removed by Voatex vortex oscillation.
4. The method for producing embryos in vitro with high reproduction rate according to claim 3, wherein: the rotary mechanism (7) is arranged on a partition plate (71) on the fixing rod (2), a plurality of air outlet holes (72) are formed in the partition plate (71), a rotating disc (73) is arranged on the fixing rod (2), a circular frame (74) is arranged on the rotating disc (73), a fan plate (75) is arranged in the circular frame (74), a limiting assembly (8) is arranged on the rotating disc (73), an air storage bin (76) is arranged in the fixing rod (2), and a first check valve (77) and a second check valve (78) are arranged on the fixing rod (2); when the air pressure plate (62) generates air pressure in the air pressure bin (61), the generated air pressure is blown to the fan plate (75) from the air outlet hole (72), and then the rotating disc (73) is driven to start rotating.
5. The method for producing embryos in vitro with high reproduction rate according to claim 4, wherein: the limiting assembly (8) comprises a ratchet ring (81) arranged on the fixing rod (2), a ratchet wheel (82) arranged on the rotating disc (73), a limiting groove (83) arranged in the fixing rod (2), a magnet (84) arranged in the limiting groove (83), a butt joint block (85) arranged on the rotating disc (73), and a magnetic wheel (86) arranged on the butt joint block (85); when the rotating disc (73) starts to rotate, the rotating disc (73) rotates singly under the action of the ratchet (81) ring and the ratchet gear (82); then, when the magnetic wheel (86) rotates, the magnetic wheel moves along the inner wall of the fixed rod (2); the magnetic wheel rotates for 45 degrees and then is butted with the limit groove (83), and when the magnetic wheel is connected with the butt joint groove (83), the magnetic wheel is butted against the magnet (84).
6. The method for producing embryos in vitro with high reproduction rate according to claim 1, wherein: the movable plate (5) comprises a phase embedding groove (51) arranged on the support plate (4), a movable plate (52) arranged in the phase embedding groove (51), a movable spring (53) arranged on the movable plate (52), a clamping block (54) arranged on the inner wall of the phase embedding groove (51), a clamping block spring (55) arranged on the clamping block (54), a support spring (56) arranged on the support plate (4) and a positioning mechanism (9) arranged on the movable plate (52); after the embryo culture dish is placed in the feeding hole (3), firstly, the mouth of the culture dish is clamped on the moving plate (52), and the moving plate (52) is driven to move downwards; then the supporting plate (4) is positioned through a positioning mechanism (9); then when unloading is needed, the culture dish is directly taken out; when the clamping block (54) is taken out, the clamping block is unlocked under the action of the clamping block spring (55).
7. The method for producing embryos in vitro with high reproduction rate according to claim 6, wherein: the positioning mechanism (9) comprises a positioning groove (91) arranged on the rotating disc (73), a positioning ring (92) arranged in the positioning groove (91), a positioning block (93) arranged at one end of the supporting plate (4), a positioning frame (94) arranged on the positioning block (93), positioning teeth (95) arranged in the positioning frame (94), positioning springs (96) arranged on the positioning teeth (95) and a driving block (97) arranged at one end of the moving plate (52); when the embryo culture dish is erected on the moving plate (52), the moving plate (52) is driven to move downwards; then, the driving block (97) is abutted against the positioning block (93), and at the moment, the driving block (97) is abutted against the positioning frame (94), so that the positioning frame (94) is separated from the positioning block (93); the further embryo culture dish drives the support plate (4) to move downwards, and then the positioning block (93) enters the positioning groove (91); at the moment, the positioning teeth (95) are buckled with the positioning ring (92).
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