CN203947105U - A kind of cell dissociation equipment - Google Patents
A kind of cell dissociation equipment Download PDFInfo
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- CN203947105U CN203947105U CN201420332806.6U CN201420332806U CN203947105U CN 203947105 U CN203947105 U CN 203947105U CN 201420332806 U CN201420332806 U CN 201420332806U CN 203947105 U CN203947105 U CN 203947105U
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- tank body
- cell dissociation
- rotating shaft
- filter screen
- cell
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Abstract
The utility model discloses a kind of cell dissociation equipment in medical equipment field, comprise the first tank body and the second tank body, described the first tank body is connected with the top of described the second tank body, the built-in filter screen post of described the second tank body, the inwall of described filter screen post and described the second tank body is fixed, described filter screen column top opening, closed bottom; Also involving vibrations motor and rotor of described cell dissociation equipment; Described rotor comprises rotating shaft and is fixed on the stirring rake of described rotating shaft bottom, the top of described rotating shaft and described vibrating motor are fixed, described rotating shaft is stretched into described the first tank body from the radial center at described the first tank body top, and described rotor carries out circle swing under the driving of described vibrating motor in described the first tank body.Its technique effect is: it can effectively prevent that in cell dissociation process, the cell on microcarrier damages, and can prevent that in cell dissociation process, filter screen post is stopped up by microcarrier.
Description
Technical field
The utility model relates to a kind of cell dissociation equipment in medical equipment field.
Background technology
Since Van Wezel in 1967 uses microcarrier (micro-carrier) system to carry out animal cell large-scale cultivation, through the development of nearly 50 years, this technology has been gradually improved at present and is ripe, and be widely used in the cellular product that cell engineering field has important practical and commercial value to produce some, as vaccine, gene engineering product etc.
At present, the microcarrier of cell is cultivated and is mainly contained two kinds, and microcarrier perfusion is cultivated and the cultivation of microcarrier stream folder.The important employing microcarrier stream of production of vaccine folder is cultivated, and can fully improve like this production of vaccine titre.Along with the continuous increase of microcarrier concentration, cell density also increases thereupon, obtain highdensity cell, can suitably increase the concentration of microcarrier.In the time that microcarrier concentration exceedes 5g/L, just must take microcarrier perfusion to cultivate, to maintain the high-density growth of cell and to ensure the nutrition supply of cell, prevent the too fast consumption due to nutrient solution nutrition, and the excessive accumulation of meta-bolites, and the cell state variation causing.Adopt microcarrier perfusion to cultivate and must use cell dissociation equipment, cell is separated with microcarrier.
Current cell dissociation equipment two classes a: class is the centrifugal cage cell dissociation of screen membrane equipment: comprise tank body and rotary sieve post, with centrifugal force separate microcarrier and the cell of rotary sieve post, its advantage is that separation efficiency is high, but tank body volume is little, the sealing property variation of tank body, rotary sieve post is easily blocked, the shearing centrifugal force producing when rotary sieve post rotation simultaneously also easily injures cell, the same with artificial digestion isolated cell, can cause cell mortality high, unit productive rate is low.Gravity sedimentation type cell dissociation equipment, the gravity vertical sedimentation system that it utilizes outside tank body, completes sepn process in conjunction with recoiling device, and its shortcoming is in the time that perfusion flow is large, and the cell in microcarrier enters after gravity vertical sedimentation system easily anoxic and nutritive deficiency; Need the artificial back-purge system of opening that oxygen and nutrition are provided.
Utility model content
The purpose of this utility model is in order to overcome the deficiencies in the prior art, and a kind of cell dissociation equipment is provided, and it can effectively prevent that in cell dissociation process, the cell on microcarrier damages, and can prevent that in cell dissociation process, filter screen post is stopped up by microcarrier.
A kind of technical scheme that realizes above-mentioned purpose is: a kind of cell dissociation equipment, comprise the first tank body and the second tank body, described the first tank body is connected with the top of described the second tank body, the built-in filter screen post of described the second tank body, the inwall of described filter screen post and described the second tank body is fixed, described filter screen column top opening, closed bottom; Also involving vibrations motor and rotor of described cell dissociation equipment;
Described rotor comprises rotating shaft and is fixed on the stirring rake of described rotating shaft bottom, the top of described rotating shaft and described vibrating motor are fixed, described rotating shaft is stretched into described the first tank body from the radial center at described the first tank body top, and described rotor carries out circle swing under the driving of described vibrating motor in described the first tank body.
Further, between the rotating shaft of described rotor and the top of described the first tank body, be provided with rubber seal.
Further, described rubber seal is tetrafluoroethylene rubber seal.
Further, the aperture of described filter screen post is 100 μ m.
Further, the radial outside of described the second tank body, is socketed with chuck, and described chuck is provided with a water inlet pipe and a rising pipe.
Further, between the top of the bottom of the sidewall of described the first tank body and described the second tank body, be connected by feed-pipe, described feed-pipe is provided with the first admission valve and the first condensation trap, on described the first admission valve, be connected with the first vapour pipe, on described the first condensation trap, be connected with the first prolong, on described the first vapour pipe and described the first prolong, be all provided with a diaphragm valve.
Further, the bottom of described the second tank body is connected with discharge nozzle, described discharge nozzle is provided with the second admission valve and the second condensation trap, on described the second admission valve, be connected with the second vapour pipe, on described the second condensation trap, be connected with the second prolong, on described the second vapour pipe and described the second prolong, be all provided with a diaphragm valve.
Adopt the technical scheme of a kind of cell dissociation equipment of the present utility model, in the first tank body, rely on the circle swing of the rotor that vibrating motor drives to make microcarrier and cellular segregation, in the second tank body, rely on microcarrier and the cell gravity settling in filter screen post to extract the technical scheme of the cell dissociation equipment of cell.Its technique effect is: it can effectively prevent that in cell dissociation process, the cell on microcarrier damages, and can prevent that in cell dissociation process, filter screen post is stopped up by microcarrier.
Brief description of the drawings
Fig. 1 is the structural representation of a kind of cell dissociation equipment of the present utility model.
Embodiment
Refer to Fig. 1, contriver of the present utility model is in order to understand the technical solution of the utility model better, below by embodiment particularly, and is described in detail by reference to the accompanying drawings:
Refer to Fig. 1, a kind of cell dissociation equipment of the present utility model comprises the first tank body 1, the second tank body 2, filter screen post 3, vibrating motor 4 and rotor 5.
3 open tops of filter screen post, closed bottom.Filter screen post 3 is fixed with the inwall of the second tank body 2 in the second tank body 2, and the aperture of filter screen post 3 is 100 μ m.
Rotor 5 comprises rotating shaft 51 and is positioned at the stirring rake 52 of rotating shaft bottom.The top of rotating shaft 51 and vibrating motor 4 are fixing, and rotating shaft 51, from the radial center at the top of the first tank body 1, along the first tank body 1 central shaft, is stretched into the first tank body 1.Therefore at vibrating motor 4 during in holding state, the rotating shaft 51 of the first tank body 1, filter screen post 3 and rotor 5 is coaxial.Vibrating motor 4 starts, along with the vibration of vibrating motor 4, and under the drive of vibrating motor 4, rotor 5, i.e. rotating shaft 51, in the first tank body 1, is carried out circle swing around the central shaft of the first tank body 1, and rotor 5 revolves round the sun around the central shaft of the first tank body 1.Along with the circle swing of rotor 5, the first tank body 1 produces and rocks, and cell is separated with microcarrier.Because separating between microcarrier and cell is to be undertaken by rocking of the first tank body 1, therefore can not produce centrifugal shearing force, can not bring the problem of the cell injury that centrifugal shearing force brings.
In order to ensure that rotating shaft 51 carries out in circle swing process, the stopping property of the first tank body 1, is provided with rubber seal 11 between the first tank body 1 top and rotating shaft 51, and what rubber seal 11 adopted is tetrafluoroethylene rubber.Carry out in circle swing process in rotating shaft 51, rubber seal 11 produces high elastic deformation along with the swing of rotating shaft 51, the gnotobasis in stopping property and first tank body 1 of guarantee the first tank body 1.
Between first bottom of tank body 1 sidewall and the top of the second tank body 2, be connected with for delivery of being connected between feed-pipe 6, the first bottoms of tank body 1 sidewall of microcarrier and cell and the top of the second tank body 2 and be rigidly connected.Feed-pipe 6 is provided with the first admission valve 61 and the first condensation trap 62, the first admission valves 61, the first condensation trap 62 are diaphragm valve.On the first admission valve 61, be connected with on the first vapour pipe 63, the first condensation traps 62 and be connected with the first prolong 64, the first vapour pipes 63 for importing steam to feed-pipe 6, the first prolong 74 is for discharging the water of condensation in feed-pipe 6.The first vapour pipe 63 is provided with the first admission diaphragm valve 65, the first prolongs 64 and is provided with the first condensation diaphragm valve 66.The first admission valve 61, the first condensation trap 62, the first admission diaphragm valve 65 and the first condensation diaphragm valve 66 are all in the time of open mode, can be to feed-pipe 6, the first tank body 1 and the second tank body 2 be carried out to sterilizing, ensure to carry in microcarrier and cell processes, the first tank body 1, the second tank body 2 and feed-pipe 6 are all in aseptic state.In the process of conveying microcarrier and cell, the first admission valve 61, the first condensation trap 62, the first admission diaphragm valve 65 and the first condensation diaphragm valve 66 are all in closing condition, in the process that ensures cell to digest, whole cell dissociation equipment is in air-tight state.
Microcarrier, in the second tank body 2, along with gravity settling, is deposited on filter screen post 3, and cell sees through the bottom of filter screen post 3, deposits to the bottom of the second tank body 2.Meanwhile, because be to be rigidly connected between the first tank body 1 and the second tank body 2, therefore the second tank body 2 rocks all the time with filter screen post 3 together with the first tank body 1, and therefore rocking of filter screen post 3 makes it be difficult for being stopped up by microcarrier.
The bottom of the second tank body 2 is connected with for delivery of the discharge nozzle 7 that is deposited to the cell of the second tank body 2 bottoms after separating.Discharge nozzle 7 is provided with the second admission valve 71 and the second condensation trap 72, the second admission valves 71, the second condensation trap 72 are diaphragm valve.On the second admission valve 71, be connected with on the second vapour pipe 73, the second condensation traps 72 and be connected with the second prolong 74, the second vapour pipes 73 for importing steam to discharge nozzle 7, the second prolong 74 is for discharging the water of condensation in discharge nozzle 7.The second vapour pipe 73 is provided with the second admission diaphragm valve 75, the second prolongs 74 and is provided with the second condensation diaphragm valve 76.The second admission valve 71, the second condensation trap 72, the second admission diaphragm valve 75 and the second condensation diaphragm valve 76 are all in the time of open mode, can be to discharge nozzle 7 and the second tank body 2 be carried out to sterilizing, ensure that discharge nozzle 7 conveyings separate in the process of rear cell, the second tank body 1, the second tank body 2 and discharge nozzle 7 are all in aseptic state.Separate in the process of rear cell in conveying, the second gas outlet valve 71, the second condensation trap 72, the second admission diaphragm valve 75 and the second condensation diaphragm valve 76 are all in closing condition, in the process that ensures cell to digest, whole cell dissociation equipment is in air-tight state.
The radial outside of the second tank body 2, is socketed with chuck 21, and chuck 21 is provided with a water inlet pipe 211 and a rising pipe 212, and the water coolant in chuck 21 circulates by water inlet pipe 211 and rising pipe 212, ensures that the interior temperature of the second tank body 2 is suitable for the growth of cell.
Those of ordinary skill in the art will be appreciated that, above embodiment is only for the utility model is described, and be not used as restriction of the present utility model, as long as within the scope of connotation of the present utility model, variation, modification to the above embodiment all will drop within the scope of claims of the present utility model.
Claims (7)
1. a cell dissociation equipment, comprises the first tank body and the second tank body, and described the first tank body is connected with the top of described the second tank body, the built-in filter screen post of described the second tank body, the inwall of described filter screen post and described the second tank body is fixed, described filter screen column top opening, closed bottom; It is characterized in that:
Also involving vibrations motor and rotor of described cell dissociation equipment;
Described rotor comprises rotating shaft and is fixed on the stirring rake of described rotating shaft bottom, the top of described rotating shaft and described vibrating motor are fixed, described rotating shaft is stretched into described the first tank body from the radial center at described the first tank body top, and described rotor carries out circle swing under the driving of described vibrating motor in described the first tank body.
2. a kind of cell dissociation equipment according to claim 1, is characterized in that: between the rotating shaft of described rotor and the top of described the first tank body, be provided with rubber seal.
3. a kind of cell dissociation equipment according to claim 2, is characterized in that: described rubber seal is tetrafluoroethylene rubber seal.
4. a kind of cell dissociation equipment according to claim 1, is characterized in that: the aperture of described filter screen post is 100 μ m.
5. a kind of cell dissociation equipment according to claim 1, is characterized in that: the radial outside of described the second tank body, be socketed with chuck, and described chuck is provided with a water inlet pipe and a rising pipe.
6. a kind of cell dissociation equipment according to claim 1, it is characterized in that: between the bottom of the sidewall of described the first tank body and the top of described the second tank body, be connected by feed-pipe, described feed-pipe is provided with the first admission valve and the first condensation trap, on described the first admission valve, be connected with the first vapour pipe, on described the first condensation trap, be connected with the first prolong, on described the first vapour pipe and described the first prolong, be all provided with a diaphragm valve.
7. a kind of cell dissociation equipment according to claim 6, it is characterized in that: the bottom of described the second tank body is connected with discharge nozzle, described discharge nozzle is provided with the second admission valve and the second condensation trap, on described the second admission valve, be connected with the second vapour pipe, on described the second condensation trap, be connected with the second prolong, on described the second vapour pipe and described the second prolong, be all provided with a diaphragm valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420332806.6U CN203947105U (en) | 2014-06-20 | 2014-06-20 | A kind of cell dissociation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420332806.6U CN203947105U (en) | 2014-06-20 | 2014-06-20 | A kind of cell dissociation equipment |
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| Publication Number | Publication Date |
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| CN203947105U true CN203947105U (en) | 2014-11-19 |
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| CN201420332806.6U Active CN203947105U (en) | 2014-06-20 | 2014-06-20 | A kind of cell dissociation equipment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107849507A (en) * | 2015-05-29 | 2018-03-27 | 迈索布拉斯特国际有限公司 | Method and apparatus for cell to be separated with microcarrier |
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2014
- 2014-06-20 CN CN201420332806.6U patent/CN203947105U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107849507A (en) * | 2015-05-29 | 2018-03-27 | 迈索布拉斯特国际有限公司 | Method and apparatus for cell to be separated with microcarrier |
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