CN112877202A - Automatic monitoring control system of edible and medicinal fungus liquid culture fermentation system - Google Patents
Automatic monitoring control system of edible and medicinal fungus liquid culture fermentation system Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention discloses an automatic monitoring and controlling system of an edible and medicinal fungus liquid culture fermentation system, which comprises a fermentation tank, wherein a foaming-defoaming combined device is arranged in the fermentation tank, the foaming-defoaming combined device comprises an outer pipe body and an inner pipe body, a plurality of partition bodies are arranged in a cavity between the outer pipe body and the inner pipe body, the partition bodies divide the cavity between the outer pipe body and the inner pipe body into a plurality of mutually independent cavities, a plurality of outer microporous regions are arranged on the outer pipe body at intervals, and a plurality of inner microporous regions are arranged on the inner pipe body at intervals; one end of the inner tube body is connected with an upper tube body with a porous structure through a switch body; the device can improve the homogeneity of dissolved oxygen distribution in the zymotic fluid, in time eliminates the top foam of zymotic fluid, improves the homogeneity of injecting acidizing fluid or alkali lye in the zymotic fluid, more accurate, the even pH of adjusting in the zymotic fluid, extracts the tail gas of zymotic fluid top, cooperates the tail gas monitoring module to carry out the analysis to the carbon dioxide in the tail gas.
Description
Technical Field
The invention relates to the technical field of edible and medicinal fungus strain culture equipment and control systems, in particular to an automatic monitoring and control system of an edible and medicinal fungus liquid culture fermentation system and a using method thereof.
Background
The industrial fermentation technology can rapidly produce a large amount of edible and medicinal fungi strains and other metabolites in a short time, solves the problems of long period and high cost of the traditional process, and brings great benefits for the production of edible and medicinal fungi, food processing and biological agents. Taking edible and medicinal fungi as an example, liquid strains are combined with popularization and application of an industrial fermentation technology, so that the production cost is greatly reduced, the production time is shortened, and important support is provided for industrial production of the strains and the fungus bags. However, the bottleneck restricting the development of the field is that the culture state and the result of the cultured strains are poor in controllability and testability in the culture process, and the development of the industry is seriously influenced. On one hand, when the dissolved oxygen in the fermentation tank is controlled, in the process of injecting air into the fermentation tank through the air distributor, the injected oxygen is unevenly distributed due to the influence of the position of the air distributor and the viscosity of the culture solution in the fermentation tank, so that the growth of strains is not facilitated; secondly, mechanical defoaming, chemical reagent defoaming or combination defoaming are mostly adopted in the existing fermentation tank, a special mechanical defoaming mechanism increases the complexity and the manufacturing cost in the fermentation tank, and the chemical reagent defoaming easily influences the quality of products; thirdly, when adjusting the pH in the fermentation cylinder, have the uneven problem of pH reagent distribution equally when adding the pH reagent through the peristaltic pump, solve the uneven problem of pH reagent and oxygen distribution and need use agitating unit for a long time to stir, complex operation, increase cost and influence production efficiency. Therefore, a high-efficiency automatic fermentation control device is needed to solve the problems that oxygen injection and pH reagent distribution in the traditional fermentation tank are uneven, defoaming machinery is complex, and chemical defoaming reagent influences product quality.
Disclosure of Invention
The invention aims to provide an automatic monitoring control system of a liquid culture fermentation system of edible and medicinal fungi and a using method thereof, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
an automatic monitoring control system of a liquid culture fermentation system for edible and medicinal fungi comprises a fermentation tank, wherein the fermentation tank is connected with an intelligent temperature control module, a pH monitoring module, a dissolved oxygen monitoring module, a tail gas monitoring module and the system control module; the intelligent temperature control module, the pH monitoring module, the dissolved oxygen monitoring module and the tail gas monitoring module are respectively in communication connection with the system control module;
preferably, the intelligent temperature control module comprises a temperature measuring device, the temperature measuring device measures the temperature in the fermentation tank in real time and transmits the temperature to a PID temperature control algorithm module in the system control module, the system control module analyzes that a temperature value received by the PID temperature control algorithm module exceeds a preset temperature range, and the system control module starts the intelligent temperature control module to linearly adjust the temperature in the fermentation tank to the preset temperature range; the pH monitoring module monitors the pH in the fermentation tank in real time and transmits the pH to the system control module, and when the pH exceeds a preset range, the system control module starts the pH adjusting module to adjust the pH in the fermentation tank to the preset range; the dissolved oxygen monitoring module comprises a dissolved oxygen measuring module, an oxygenation module and a pressure monitoring module; the system comprises a system control module, a dissolved oxygen measuring module, an oxygenation module, a pressure monitoring module and a gas releasing valve, wherein the dissolved oxygen measuring module measures the dissolved oxygen value in the fermentation tank in real time and transmits the value to the system control module; the tail gas monitoring module collects tail gas in the fermentation tank, analyzes the content of carbon dioxide in the tail gas and transmits the content value of the carbon dioxide to the system control module;
preferably, a foaming-defoaming combined device is arranged in the fermentation tank, the foaming-defoaming combined device comprises an outer pipe body, an inner pipe body is nested in the outer pipe body, a plurality of partition bodies are arranged in a cavity between the outer pipe body and the inner pipe body, the partition bodies divide the cavity between the outer pipe body and the inner pipe body into a plurality of mutually independent cavities, a plurality of outer microporous regions are arranged on the outer pipe body at intervals, and a plurality of inner microporous regions are arranged on the inner pipe body at intervals; one end of the inner tube body is connected with an upper tube body with a porous structure through a switch body; rotating the outer tube and/or the inner tube, wherein the inner micropore area at the lower part of the inner tube is communicated with the outer micropore area at the lower part of the outer tube through the chamber, and the inner micropore area at the upper part of the inner tube is not communicated with the outer micropore area at the upper part of the outer tube; rotating the outer pipe body and/or the inner pipe body again, wherein the inner micropore area at the upper part of the inner pipe body is communicated with the outer micropore area at the upper part of the outer pipe body through the cavity, and the inner micropore area at the lower part of the inner pipe body is not communicated with the outer micropore area at the lower part of the outer pipe body; and rotating the outer pipe body and/or the inner pipe body again, wherein the inner micropore areas at the upper part and the lower part of the inner pipe body are not communicated with the outer micropore areas at the upper part and the lower part of the outer pipe body, the switch body is opened, and the inner pipe body is communicated with the upper pipe body.
Preferably, the inner tube body is connected with an air pump through a first branch tube, and the air pump blows pulsed disinfection and purification air into the inner tube body through the first branch tube; the inner pipe body is connected with a peristaltic pump of the pH adjusting module through a second branch pipe.
Preferably, the inner pipe body is connected with the tail gas monitoring module through a third branch pipe and a gas pump.
Preferably, a defoaming electrode is arranged above the inside of the fermentation tank, and the height of the bottommost part of the defoaming electrode is not greater than the height of the topmost part of the upper pipe body.
Preferably, the number of the foaming-defoaming combined devices is not less than two, and the foaming-defoaming combined devices are arranged in a circular matrix in the fermentation tank.
Preferably, the inner wall and the outer wall of the outer tube and the inner tube are both provided with a reed capable of freely vibrating, and the reed is provided with a plurality of micropores.
Preferably, the outer tube is movably connected with the body of the fermentation tank, and the outer tube is rotated to adjust the position of the outer micropore area above the outer tube in the fermentation tank.
Preferably, the density of micropores in the outer microporous region below the outer tube is greater than the density of micropores in the outer microporous region above the outer tube.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention realizes the structure that the lower outer micropore area of the foaming-defoaming combined device is communicated with the lower inner micropore area and the upper outer micropore area is not communicated with the upper inner micropore area by arranging the structures of the foaming-defoaming combined device, the outer pipe body, the inner pipe body, the partition body, the chamber, the outer micropore area, the inner micropore area and the like and by the relative rotation of the outer pipe body and the inner pipe body, and at the moment, air can be injected into the lower part of fermentation liquor through the outer pipe body, the inner pipe body, the outer micropore area and the inner micropore area to improve the dissolved oxygen content of the lower part; meanwhile, the upper outer micropore area of the foaming-defoaming combined device is communicated with the upper inner micropore area, the lower outer micropore area is not communicated with the lower inner micropore area structure, and air can be injected into the upper part of the fermentation liquor through the outer pipe body, the inner pipe body, the outer micropore area and the inner micropore area, so that the dissolved oxygen in the upper part is increased; the two are combined, and the air is injected into the fermentation liquor in a segmented manner, so that the distribution uniformity of air bubbles can be improved when the air bubbles are injected into the fermentation liquor, the uniformity of the dissolved oxygen in the fermentation liquor is further ensured, and the product quality is improved.
2. The invention realizes that the upper and lower outer microporous areas of the foaming-defoaming combined device are not communicated with the upper and lower inner microporous area structures through the arrangement of the foaming-defoaming combined device, the outer pipe body, the inner pipe body, the partition body, the cavity, the outer microporous area, the inner microporous area, the switch body, the upper pipe body and other structures through the relative rotation of the outer pipe body and the inner pipe body, and at the moment, high-pressure air can be blown to the upper part of fermentation liquor through the outer pipe body and the upper pipe body, so that the foam above the fermentation liquor is dispersed and broken, the defoaming effect is achieved, the complicated defoaming mechanism in the fermentation tank is simplified, the use amount of chemical defoaming reagents is avoided or reduced, and the product quality is improved.
3. The foaming-defoaming combined device, the outer tube body, the inner tube body, the partition body, the chamber, the outer micropore area, the inner micropore area and other structures are arranged, and through the relative rotation of the outer tube body and the inner tube body, the structure that the lower outer micropore area of the foaming-defoaming combined device is communicated with the lower inner micropore area and the upper outer micropore area and is not communicated with the upper inner micropore area is realized, the structure that the upper outer micropore area is communicated with the upper inner micropore area and the lower outer micropore area and is not communicated with the lower inner micropore area is realized, at the moment, acid liquor or alkali liquor is mixed with high-pressure gas through a peristaltic pump, and the acid liquor or alkali liquor is uniformly sprayed into fermentation liquor on the upper part and the lower part through the outer micropore area, the inner micropore area, the outer tube body and the inner tube body, so that the distribution uniformity of the acid liquor or alkali liquor in the fermentation liquor.
4. The device is provided with a foaming-defoaming combined device, an outer pipe body, an inner pipe body, a partition body, a cavity, an outer micropore area, an inner micropore area and other structures, the upper and lower outer micropore areas of the foaming-defoaming combined device are not communicated with the upper and lower inner micropore area structures through the relative rotation of the outer pipe body and the inner pipe body, and at the moment, tail gas above fermentation liquor can be extracted outwards through the outer micropore area, the inner pipe body and the upper pipe body to be detected, the content of carbon dioxide in the tail gas is analyzed, and guidance is provided for mastering the fermentation condition of strains in the fermentation liquor.
5. Through setting up intelligent temperature control module, pH monitoring module, dissolved oxygen monitoring module, tail gas monitoring module and system control module, at the bacterial of cultivateing in-process, can guarantee that bacterial culture state and result are controllable testable, degree of automation is high, great reduction manufacturing cost, shortened the production time, provide important support for the industrial production of bacterial and fungus package.
Drawings
FIG. 1 is a schematic cross-sectional view of the overall structure of the present invention;
FIG. 2 is an enlarged schematic view of the structure at A in the present invention;
FIG. 3 is a schematic structural diagram of a lower outer microporous region communicating with a lower inner microporous region and an upper outer microporous region not communicating with an upper inner microporous region of the combined foaming and defoaming apparatus of the present invention;
FIG. 4 is a schematic structural diagram of the foaming-defoaming combination unit of the present invention, in which the upper outer microporous region is communicated with the upper inner microporous region and the lower outer microporous region is not communicated with the lower inner microporous region;
FIG. 5 is a schematic structural view of the foaming-defoaming combination unit of the present invention, in which the upper and lower outer microporous regions are not communicated with the upper and lower inner microporous regions;
FIG. 6 is a flow chart of a system control module of the fermenter according to the present invention;
FIG. 7 is a flow chart of the control system for controlling the amount of dissolved oxygen in the fermenter according to the present invention.
In the figure: 1. a fermentation tank; 2. an intelligent temperature control module; 3. a pH monitoring module; 4. a dissolved oxygen monitoring module; 5. an exhaust gas monitoring module; 6. a system control module; 7. foaming-defoaming combined equipment; 701. an outer tubular body; 702. an inner tube body; 703. a separator; 704. a chamber; 705. an outer microporous region; 706. an inner microporous region; 707. a switch body; 708. a pipe body is arranged; 709. a reed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 6-7, an automatic monitoring and control system for a liquid culture fermentation system of edible and medicinal fungi comprises a fermentation tank 1, wherein the fermentation tank 1 is connected with an intelligent temperature control module 2, a pH monitoring module 3, a dissolved oxygen monitoring module 4, a tail gas monitoring module 5 and a system control module 6; the intelligent temperature control module 2, the pH monitoring module 3, the dissolved oxygen monitoring module 4 and the tail gas monitoring module 5 are respectively in communication connection with the system control module 6, so that the data of each module can be transmitted to the system control module 6, and the whole process flow of the fermentation system can be automatically and unmanned controlled; the fermentation tank 1 comprises an inner layer and an outer interlayer, wherein the inner layer is used for storing fermentation liquor to complete the fermentation process. The inner layer of the fermentation tank 1 is provided with a stirring device, wherein the stirring device is provided with two stages, the first-stage stirring device adopts an inclined-blade stirring paddle, the second-stage stirring device adopts a straight-blade stirring paddle, the combination of the first-stage stirring device and the second-stage stirring device can improve the level and the up-down flow of the fermentation liquor, and promote the uniform distribution of nutrient substances, dissolved oxygen, pH reagents and the like in the fermentation liquor.
The intelligent temperature control module 2 comprises a temperature measuring device, the temperature measuring device of the intelligent temperature control module 2 is an epoxy sealing structure temperature sensor and has high stability and accuracy, the temperature measuring device measures the temperature in the fermentation tank 1 in real time and transmits the temperature to the PID temperature control algorithm module in the system control module 6, the system control module 6 analyzes that the temperature value received by the PID temperature control algorithm module exceeds a preset temperature range, and the system control module 6 starts the intelligent temperature control module 2 to linearly regulate the temperature in the fermentation tank 1 to be within the preset temperature range; in the process of fermentation in the fermentation tank 1, the steam pipeline for adjusting the internal temperature of the fermentation tank 1 is connected in the outer interlayer of the fermentation tank 1, and the intelligent temperature control module 1 further comprises a pneumatic control valve arranged on the steam pipeline. When the temperature sensor detects the temperature in the fermentation tank 1 and transmits the temperature to the PID temperature control algorithm module in the system control module 6, when the temperature in the fermentation tank 1 is higher than or lower than a preset range, the system control module 6 opens a pneumatic regulating valve on a steam pipeline, hot water or cold water enters an outer interlayer of the fermentation tank 1 and flows in the outer interlayer and then flows out, heat or cold is transmitted to fermentation liquor in the fermentation tank 1, and simultaneously, under the stirring of a stirring device, the temperature of the fermentation liquor in the fermentation tank 1 is linearly increased or decreased so as to ensure the stability of the fermentation liquor; the temperature sensor, the water inlet and outlet temperature and the stirring device are controlled, so that the precision of the temperature in the fermentation tank is controlled to be +/-0.5 ℃, and a stable fermentation environment is provided for the fermentation process of the fermentation liquid.
The original culture medium of the edible and medicinal fungi has stable pH after being prepared, whether the culture medium meets the standard or not can be monitored by a pH monitoring system, and the pH is an important parameter index for whether the production is finished or not. The pH monitoring module 3 monitors the pH value in the fermentation tank 1 in real time and transmits the pH value to the system control module 6, and when the pH value exceeds a preset range, the system control module 6 starts the pH adjusting module to adjust the pH value in the fermentation tank to the preset range; the pH monitoring module comprises a special pH electrode for measuring the pH of the fermentation liquor, and the pH electrode adopts a Switzerland pH electrode, so that the pH monitoring module has higher stability and accuracy; the pH adjusting module comprises a peristaltic pump and an acid and alkali adjusting container which correspond to the acid and alkali solutions, the inner pipe body 702 is connected with the peristaltic pump of the pH adjusting module through the second branch pipe, when the pH of the fermentation liquor needs to be adjusted, the peristaltic pump for adding the acid solution or the peristaltic pump for adding the alkali solution pumps the acid solution or the alkali solution into the second branch pipe, and the foaming-defoaming combined device 7 is used for completing the addition of the acid solution or the alkali solution, so that the uniformity of the acid solution or the alkali solution in the fermentation liquor can be improved.
Along with the growth of the strains, when the biomass is large, the original ventilation quantity cannot meet the requirement of oxygen consumption of the strains. The pressure of the tank body can rise when the ventilation quantity is increased, and safety and operation risks exist, so the invention is provided with a combined air inflow-pressure-dissolved oxygen monitoring system. On the one hand, the air input of the fermentation tank 1 is accurately controlled, and the pressure in the fermentation tank 1 is measured, so that the pressure in the fermentation tank 1 is ensured on the basis of ensuring that enough dissolved oxygen is provided for fermentation liquor, and the safety production coefficient is improved. The dissolved oxygen monitoring module 4 comprises a dissolved oxygen measuring module, an oxygenation module and a pressure monitoring module; the dissolved oxygen measuring module comprises a DO electrode for detecting dissolved oxygen in the fermentation liquor, and the DO electrode adopts a Switzerland Mettler DO electrode, so that the stability and the accuracy are high, and the maintenance amount is small; the oxygenation module comprises an air pump, a filtering sterilizer and part of components in the foaming-defoaming combined device 7, the air pump pumps the air outside the fermentation tank 1 into the fermentation tank for pressurization, the pressurized air enters the fermentation tank through the part of components in the foaming-defoaming combined device 7 after being filtered and sterilized by the filtering sterilizer, enough oxygen is supplied to the fermentation liquid, and the normal growth of strains in the fermentation liquid is ensured. In automatic production process, dissolved oxygen measuring module measures the interior dissolved oxygen value of fermentation cylinder in real time and transmits to system control module 6, when the dissolved oxygen numerical value in the fermentation cylinder is less than the predetermined scope, system control module 6 starts the oxygenation module, let in the air after the disinfection filters in 1 to the fermentation cylinder, pressure monitoring module real-time measurement fermentation cylinder 1 in simultaneously gives system control module 6 pressure and transmission, when the big setting value of pressure in 1 fermentation cylinder, system control module 6 reports to the police and opens the bleed valve, prevent that 1 internal gas pressure of fermentation cylinder is too big, cause safety risk.
The tail gas monitoring module 5 collects tail gas in the fermentation tank and analyzes the content of carbon dioxide in the tail gas, then the carbon dioxide content value is transmitted to the system control module 6, in the specific production process, the device can utilize a foaming-defoaming combined device 7 in the fermentation tank 1 to absorb gas above fermentation liquid in the fermentation tank 1 and input the gas into the tail gas monitoring module 5, the content of carbon dioxide in the tail gas is measured and calculated through the tail gas monitoring module 5 to obtain the metabolic rate of edible and medicinal fungus strains in the fermentation tank 1 at present, so that the growth cycle of the current state of the strains can be judged, and accurate guidance is provided for production.
The system control module 6 adopts a PLC lower computer control system, selects an industrial touch control integrated machine, a programming control system, a liquid crystal touch screen and a full Chinese menu configuration. The operator directly clicks the screen with a hand, executes the operation, and inputs the setting. Providing and programming a human-machine interface friendly. The system control module 6 collects and records the temperature, the dissolved oxygen, the pH, the tail gas carbon dioxide and the gas flow in the fermentation tank 1, sets temperature control parameters, displays digital display data in real time, records reports in real time and draws curves of all parameters in real time. The system control module 6 is provided with an upper limit value and a lower limit value for each parameter in the fermentation tank, and the control system can generate buzzing alarm when abnormality occurs. The system control module 6 is provided with a multi-level password control system, and different levels of authorities can prevent parameters from being changed by mistake due to misoperation of operators.
After the culture medium is poured into the fermentation tank, the morphological parameters in the fermentation tank 1 are obtained through the system control module 6 and the pH monitoring system determines that the culture medium meets the standard. Set parameters are input through the system control module 6, a sterilization program is started in a one-key mode, and the fermentation tank 1 is automatically cooled after sterilization. The on-site operator can call out the history records and curves of temperature, pressure, pH value, etc. through the system control module 6 to confirm that the whole sterilization process is correct. After inoculation, the system control module 6 monitors the relevant parameters according to the set parameters. The temperature control system adjusts the culture temperature, if parameters are abnormal, the lower computer control system buzzes to give an alarm, and an operator processes the alarm. In the culture process of the fermentation tank 1, an operator can observe real-time parameters, historical/real-time reports, historical/real-time curves and the like of each system of the tank body through the liquid crystal display screen at any time to judge the fermentation condition and state, and can also roughly judge quantitative parameters such as nutrient consumption and the like through an air input curve and a tail gas carbon dioxide curve. After the culture is finished, the operator can store the related data for later traceability.
As shown in fig. 1-5, a combined foaming and defoaming device 7 is disposed in the fermentation tank 1, and the combined foaming and defoaming device 7 has different structural compositions according to its internal components, and has different functions, and in this embodiment, is mainly used for: (1) the foaming function is that the air introduced from the outside of the fermentation tank 1 forms tiny bubbles and is uniformly distributed in the fermentation liquor at the upper layer and the lower layer, so that the uniformity of dissolved oxygen in the fermentation liquor is improved; (2) the defoaming function, when need not annotate oxygen to fermentation cylinder 1 in, the device can blow highly-compressed air to the foam collecting region of fermentation liquid top in fermentation cylinder 1, makes the foam break, reaches the effect of defoaming.
The number of the foaming-defoaming combined devices 7 is not less than two, and the devices are arranged in the fermentation tank 1 in an annular matrix, so that the uniformity of the device in oxygen injection into fermentation liquor can be improved, and the effect of eliminating foam above the fermentation liquor can also be improved.
The foaming-defoaming combined device 7 comprises an outer tube body 701, the outer tube body 701 is soaked in fermentation liquor, and air is sprayed to the fermentation liquor through an outer micropore area 705 above the outer tube body 701, so that the dissolved oxygen in the fermentation liquor is increased; an inner pipe body 702 is nested in the outer pipe body 701, the inner pipe body 702 is connected with an air pump through a first branch pipe, the air pump blows pulsed disinfection and purification air into the inner pipe body 702 through the first branch pipe, on one hand, large bubbles formed when the pulsed air passes through an inner micropore area 706 on the inner pipe body 702 and an outer micropore area 705 on the outer pipe body 701 become small bubbles, and then the stirring effect of a stirring device is combined to enable the small bubbles containing oxygen to be uniformly distributed in fermentation liquor, so that the uniformity of dissolved oxygen in the fermentation liquor is improved; on the other hand, the pulsed air flow collides with the inner microporous region 706, the outer microporous region 705 and the reed 709, so that larger air bubbles can be gradually reduced, the micro bubbles are uniformly distributed in the fermentation broth, and the absorption of strains in the fermentation broth to oxygen is facilitated; the secondary pulsating air flow impacts the inner microporous region 706, the outer microporous region 705 and the reed 709, so that the microporous region 706, the outer microporous region 705 and the reed 709 can vibrate, and the fermentation liquid is prevented from blocking the microporous region 706 and the outer microporous region 705 after a long time, and the ventilation functions of the two are not influenced. The upper ends of the outer tube 701 and the inner tube 702 are sealed to prevent air leakage at the ends during ventilation.
A plurality of partition bodies 703 are arranged in a cavity between the outer pipe body 701 and the inner pipe body 702, the cavity between the outer pipe body 701 and the inner pipe body 702 is divided into a plurality of mutually independent cavities 704 by the partition bodies 703, and the partition bodies 703 have strong elasticity, can have good sealing performance and good wear resistance, and are not easily damaged in the long-time relative movement process of the outer pipe body 701 and the inner pipe body 702. A plurality of outer micropore areas 705 are arranged on the outer tube body 701 at intervals, the outer micropore areas 705 on the upper part and the lower part of the outer tube body 701 are asymmetrically distributed along the axial direction, and the outer micropore areas 705-no outer micropore areas 705 are distributed on the outer tube body 701 on the upper part or the lower part; a plurality of inner micropore areas 706 are arranged on the inner pipe body 702 at intervals, the inner micropore areas 706 on the upper part and the lower part of the inner pipe body 702 are asymmetrically distributed along the axial direction, and the inner micropore area 706-without inner micropore area 706-with inner micropore area 706-without inner micropore area 706 are distributed on the inner pipe body 702 on the upper part or the lower part; by separating the body 703 and the different chambers 704 formed by the body, and combining the relative rotation of the outer body 701 and the inner body 702, the corresponding upper part and lower part of the outer body 701 and the inner body 702 respectively realize the combination of the outer microporous region 705-inner microporous region 706 communicated with each other and the outer microporous region 705-inner microporous region 706 not communicated with each other, when the outer microporous region 705-inner microporous region 706 are communicated with each other, the fermentation liquor at the lower part can be injected with oxygen and acid and alkali liquid, or the fermentation liquor at the upper part can be injected with oxygen and acid and alkali liquid, and the combination of the outer microporous region 705-inner microporous region 706 not communicated with each other can defoam the foam above the fermentation liquor.
The inner walls and the outer walls of the outer pipe body 701 and the inner pipe body 702 are respectively provided with a reed 709 which can freely vibrate, the reed 709 is provided with a plurality of micropores, the design of the reed 709 and the micropores on the reed 709 is that when pulsating sterilizing and purifying air is blown into the inner pipe body 702 through the first branch pipe by the air pump, the pulsating air can enable the reed 709 to vibrate freely, on one hand, the free vibration of the reed 709 is combined with the micropore structure of the reed, so that large air bubbles can be changed into small air bubbles, and the small air bubbles are uniformly distributed in fermentation liquor, so that oxygen can be absorbed by strains; and then the free vibration of the reed 709 can drive the micropore area 706 and the outer micropore area 705 to vibrate, so that the fermentation liquor is prevented from blocking the micropore area 706 and the outer micropore area 705 after a long time, and the ventilation functions of the two areas are prevented from being influenced.
When oxygen is injected into the fermentation liquor in the fermentation tank 1 by using the foaming-defoaming combined device 7, when the fermentation liquor is viscous, and small air bubbles cannot smoothly float to the upper part of the fermentation liquor after being blown from the lower part of the fermentation liquor, so that the whole distribution of the bubbles in the fermentation liquor is uneven, the fermentation liquor can be divided into an upper part and a lower part, and air is respectively supplemented.
When oxygen is supplemented to the fermentation liquor at the lower part, the outer pipe body 701 and/or the inner pipe body 702 are/is rotated, the inner micropore area 706 at the lower part of the inner pipe body 702 is communicated with the outer micropore area 705 at the lower part of the outer pipe body 701 through the chamber 704, the inner micropore area 706 at the upper part of the inner pipe body 702 is not communicated with the outer micropore area 705 at the upper part of the outer pipe body 701, the switch body 707 is closed, at the moment, pulsed disinfection and purification air is blown into the inner pipe body 702 through the first branch pipe and the air pump, and the air uniformly enters the fermentation liquor at the lower part through the inner micropore area 706 at the lower part, the chamber 704 and the outer micropore area 705 at the lower part, so that the air is independently.
When oxygen is supplemented to the fermentation liquor at the upper part, the outer pipe body 701 and/or the inner pipe body 702 are/is rotated again, the inner micropore area 706 at the upper part of the inner pipe body 702 is communicated with the outer micropore area 705 at the upper part of the outer pipe body 701 through the cavity 704, and the inner micropore area 706 at the lower part of the inner pipe body 702 is not communicated with the outer micropore area 705 at the lower part of the outer pipe body 701; when the switch body 707 is closed, the pulsating sterilization and purification air is blown into the inner tube body 702 through the first branch tube and the air pump, and the air uniformly enters the upper fermentation liquid through the upper inner microporous region 706, the chamber 704 and the upper outer microporous region 705, so that the air is independently injected into the upper fermentation liquid, and the uniformity of dissolved oxygen in the upper fermentation liquid is improved.
When air bubbles are injected into the lower fermentation liquid independently, a part of the bubbles in the lower fermentation liquid can float up to the upper fermentation liquid under the action of buoyancy according to different viscosities of the fermentation liquids, and the density of micropores on the outer micropore area 705 below the outer tube body 701 is larger than that of micropores on the outer micropore area 705 above the outer tube body, so that when air is blown into the lower fermentation liquid and the upper fermentation liquid respectively, the air amount entering the upper fermentation liquid is smaller than that entering the lower fermentation liquid under the condition that the air blowing time into the inner tube body 702 is the same. Because a part of bubbles in the lower layer fermentation liquor can float upwards to the upper layer fermentation liquor under the action of buoyancy force and are mixed with air bubbles in the upper layer fermentation liquor, the density of micropores on the outer micropore area 705 below the outer pipe body 701 is greater than that of micropores on the outer micropore area 705 above, the air quantity introduced into the upper layer fermentation liquor and the lower layer fermentation liquor can be ensured to be equal or similar to the maximum degree, and simultaneously, the dissolved oxygen quantity in the upper layer fermentation liquor and the lower layer fermentation liquor is equal or similar to each other under the stirring action of the stirring device, so that the quality difference of the fermentation liquor products is reduced to the maximum degree, and the stability of the product quality is ensured.
The outer tube 701 is movably connected with the body of the fermentation tank 1, and the outer tube 701 is rotated to adjust the position of the upper outer microporous region 705 in the fermentation tank 1. The rotation of the outer pipe 701 and/or the inner pipe 702 can change the positions of the outer microporous region 705 and the inner microporous region 706, and further adjust the positions and regions of the air bubbles or the acid-base solution sprayed from the outer microporous region 705, so as to promote the uniformity of the distribution of the air bubbles or the acid-base solution. The outer pipe 701 and the inner pipe 702 are driven by different stepping motors respectively, the rotating angles of the outer pipe 701 and the inner pipe 702 can be controlled, different combination modes of an outer micropore area 705 on the outer pipe 701 and an inner micropore area 706 on the inner pipe 702 are realized, and different functions are further realized.
When the foaming-defoaming combined device 7 is used for eliminating foam accumulated above fermentation liquor in the fermentation tank 1, one end of the inner pipe body 702 is connected with an upper pipe body 708 with a porous structure through a switch body 707, and the end of the upper pipe body 708 is in a blocking state; the switch body 707 is an electromagnetic valve, which can realize automatic control of the switch body 707. When the outer pipe 701 and/or the inner pipe 702 are/is rotated again, the inner microporous region 706 at the upper part and the lower part of the inner pipe 702 are not communicated with the outer microporous region 705 at the upper part and the lower part of the outer pipe 701, and air can not enter the fermentation liquid through the inner pipe 702 and the outer pipe 701. At this time, the switch 707 is opened, the inner tube 702 is communicated with the upper tube 708, the upper tube 708 is located above the fermentation liquid, the high pressure air introduced into the inner tube 702 enters the upper tube 708 and is ejected out through the porous structure on the upper tube 708, and the bubbles collected above the fermentation liquid are impacted and scattered to be broken, so that the defoaming effect is achieved.
The defoaming electrode is arranged at the upper part in the fermentation tank 1, and the height of the bottommost part of the defoaming electrode is not more than the height of the topmost part of the upper pipe body 708. When the bubbles above the fermentation liquor are accumulated to the height of the defoaming electrode, the foaming-defoaming combined device 7 is started to perform defoaming work, and simultaneously the defoaming electrode and the height of the upper pipe body 708 are controlled, so that the bubbles can be timely defoamed, and the accumulation of the bubbles is prevented.
Example 2
The inner pipe 702 is connected with the peristaltic pump of the pH adjusting module through a second branch pipe, when the pH of the fermentation liquid needs to be adjusted, the peristaltic pump for adding acid liquid or the peristaltic pump for adding alkali liquid pumps the acid liquid or the alkali liquid into the second branch pipe, and the foaming-defoaming combined device 7 can be used for respectively adding the acid liquid or the alkali liquid into the fermentation liquid below and the fermentation liquid above.
When acid liquor or alkali liquor is supplemented to the fermentation liquor at the lower part to adjust the pH value of the fermentation liquor at the lower part, the outer pipe body 701 and/or the inner pipe body 702 are/is rotated, the inner micropore area 706 at the lower part of the inner pipe body 702 is communicated with the outer micropore area 705 at the lower part of the outer pipe body 701 through the chamber 704, the inner micropore area 706 at the upper part of the inner pipe body 702 is not communicated with the outer micropore area 705 at the upper part of the outer pipe body 701, the switch body 707 is closed, and at the moment, pulsating sterilization and purification gas is blown into the inner pipe body, simultaneously, acid liquor or alkali liquor is pumped into the inner tube body 702 through the peristaltic pump and the second branch tube, and the sterilized and purified gas with pressure is mixed with the acid liquor or the alkali liquor, uniformly sprayed into the lower fermentation broth through the lower inner microporous region 706, the chamber 704 and the lower outer microporous region 705, the lower fermentation liquor is independently injected with the acid liquor or the alkali liquor, and the uniformity of the acid liquor or the alkali liquor in the lower fermentation liquor is improved.
When acid liquor or alkali liquor is supplemented to the fermentation liquor at the upper part to adjust the pH value of the fermentation liquor at the lower part, the outer pipe body 701 and/or the inner pipe body 702 are/is rotated, the inner micropore area 706 at the upper part of the inner pipe body 702 is communicated with the outer micropore area 705 at the upper part of the outer pipe body 701 through the chamber 704, the inner micropore area 706 at the lower part of the inner pipe body 702 is not communicated with the outer micropore area 705 at the lower part of the outer pipe body 701, the switch body 707 is closed, and at the moment, pulsating sterilization and purification gas is blown into the inner pipe body, simultaneously, acid liquor or alkali liquor is pumped into the inner tube body 702 through the peristaltic pump and the second branch tube, and the sterilized and purified gas with pressure is mixed with the acid liquor or the alkali liquor, uniformly sprayed into the upper fermentation broth through the upper inner microporous region 706, the chamber 704 and the upper outer microporous region 705, the upper fermentation liquor is independently injected with the acid liquor or the alkali liquor, and the uniformity of the acid liquor or the alkali liquor in the lower fermentation liquor is improved. When high-pressure gas is used for mixing the acid liquid or the alkali liquid, inert gas which does not influence the quality of the fermentation liquid can be selected in order to prevent the gas from entering the fermentation liquid along with the acid liquid or the alkali liquid to influence the fermentation liquid.
Example 3
The inner pipe body 702 is connected with the tail gas monitoring module 5 through a third branch pipe and a gas pump, and can utilize a foaming-defoaming combined device 7 to complete the sampling work of tail gas above fermentation liquor.
When the tail gas above the fermentation liquid is sampled, the outer pipe body 701 and/or the inner pipe body 702 are/is rotated, the inner microporous region 706 at the upper part and the lower part of the inner pipe body 702 are not communicated with the outer microporous region 705 at the upper part and the lower part of the outer pipe body 701, the switch body 707 is opened at the moment, the inner pipe body 702 is communicated with the upper pipe body 708, the upper pipe body 708 is positioned above the fermentation liquid, the air pump pumps outwards to remove the fermentation liquid accumulated in the outer pipe body 701 and the inner pipe body 702 firstly, then the tail gas above the fermentation liquid is pumped into the tail gas monitoring module 5, the tail gas monitoring module 5 analyzes and calculates the content of carbon dioxide in the tail gas, and the tail gas collection work in the fermentation tank 1 is completed by using the. In summary, the outer pipe 701 and the inner pipe 702 of the combined foaming-defoaming device 7 are rotated to make the outer microporous region 705 and the inner microporous region 706 in different combined structures, so that the following functions can be realized: (1) air is injected into the fermentation liquor at the lower part and the fermentation liquor at the upper part respectively, so that the uniformity of air bubbles entering and distributing in the fermentation liquor is improved, and the uniformity of dissolved oxygen distribution in the fermentation liquor is further improved; (2) aerating the foam above the fermentation liquor at high pressure to eliminate the foam above the fermentation liquor; (3) respectively injecting acid liquor or alkali liquor into the lower part of fermentation liquor and the upper part of fermentation liquor, so that the uniformity of the acid liquor or the alkali liquor in the fermentation liquor is improved, and the pH value in the fermentation liquor is more accurately and uniformly adjusted; (4) and extracting the tail gas above the fermentation liquor, and analyzing the carbon dioxide in the tail gas by matching with a tail gas monitoring module. The foaming-defoaming combined device 7 is formed by a simple structure, has rich functions, greatly reduces the complexity of the whole device, reduces the manufacturing cost of equipment, and has obvious technical effect and economic benefit.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (10)
1. An automatic monitoring control system of an edible and medicinal fungus liquid culture fermentation system comprises a fermentation tank (1), and is characterized in that the culture fermentation tank (1) is connected with an intelligent temperature control module (2), a pH monitoring module (3), a dissolved oxygen monitoring module (4), a tail gas monitoring module (5) and a system control module (6); the intelligent temperature control module (2), the pH monitoring module (3), the dissolved oxygen monitoring module (4) and the tail gas monitoring module (5) are respectively in communication connection with the system control module (6).
2. The automatic monitoring and control system for the liquid culture fermentation system of edible and medicinal fungi as claimed in claim 1, wherein: the intelligent temperature control module (2) comprises a temperature measuring device, the temperature measuring device measures the temperature in the fermentation tank (1) in real time and transmits the temperature to a PID temperature control algorithm module in the system control module (6), the system control module (6) analyzes that the temperature value received by the PID temperature control algorithm module exceeds a preset temperature range, and the system control module (6) starts the intelligent temperature control module (2) to linearly adjust the temperature in the fermentation tank (1) to be within the preset temperature range; the pH monitoring module (3) monitors the pH in the fermentation tank (1) in real time and transmits the pH to the system control module (6), and when the pH exceeds a preset range, the system control module (6) starts the pH adjusting module to adjust the pH in the fermentation tank (1) to be within the preset range; the dissolved oxygen monitoring module (4) comprises a dissolved oxygen measuring module, an oxygenation module and a pressure monitoring module; the dissolved oxygen measuring module measures a dissolved oxygen value in the fermentation tank (1) in real time and transmits the dissolved oxygen value to the system control module (6), when the dissolved oxygen value in the fermentation tank (1) is lower than a preset range, the system control module (6) starts the oxygenation module, air after disinfection and filtration is introduced into the fermentation tank (1), meanwhile, the pressure monitoring module measures the pressure in the fermentation tank (1) in real time and transmits the pressure to the system control module (6), and when the pressure in the fermentation tank (1) is larger than a set value, the system control module (6) alarms and opens a gas release valve; the tail gas monitoring module (5) collects tail gas in the fermentation tank, analyzes the content of carbon dioxide in the tail gas and transmits the carbon dioxide content value to the system control module (6).
3. The automatic monitoring and control system for the liquid culture fermentation system of edible and medicinal fungi as claimed in claim 1, wherein: a foaming-defoaming combined device (7) is arranged in the fermentation tank (1), the foaming-defoaming combined device (7) comprises an outer pipe body (701), an inner pipe body (702) is nested in the outer pipe body (701), a plurality of partition bodies (703) are arranged in a cavity between the outer pipe body (701) and the inner pipe body (702), the partition bodies (703) divide the cavity between the outer pipe body (701) and the inner pipe body (702) into a plurality of mutually independent chambers (704), a plurality of outer microporous regions (705) are arranged on the outer pipe body (701) at intervals, and a plurality of inner microporous regions (706) are arranged on the inner pipe body (702) at intervals; one end of the inner tube body (702) is connected with an upper tube body (708) with a porous structure through a switch body (707);
rotating the outer pipe body (701) and/or the inner pipe body (702), wherein an inner microporous region (706) at the lower part of the inner pipe body (702) is communicated with an outer microporous region (705) at the lower part of the outer pipe body (701) through a chamber (704), and the inner microporous region (706) at the upper part of the inner pipe body (702) is not communicated with the outer microporous region (705) at the upper part of the outer pipe body (701); rotating the outer pipe body (701) and/or the inner pipe body (702) again, wherein an inner microporous region (706) at the upper part of the inner pipe body (702) is communicated with an outer microporous region (705) at the upper part of the outer pipe body (701) through a chamber (704), and the inner microporous region (706) at the lower part of the inner pipe body (702) is not communicated with the outer microporous region (705) at the lower part of the outer pipe body (701); and the outer pipe body (701) and/or the inner pipe body (702) are/is rotated again, the inner microporous area (706) at the upper part and the lower part of the inner pipe body (702) are not communicated with the outer microporous area (705) at the upper part and the lower part of the outer pipe body (701), the switch body (707) is opened, and the inner pipe body (702) is communicated with the upper pipe body (708).
4. The automatic monitoring and control system for the liquid culture fermentation system of edible and medicinal fungi as claimed in claim 1, wherein: the inner pipe body (702) is connected with an air pump through a first branch pipe, and the air pump blows pulsed disinfection and purification air into the inner pipe body (702) through the first branch pipe; the inner pipe body (702) is connected with the peristaltic pump of the pH adjusting module through a second branch pipe.
5. The automatic monitoring and control system for liquid culture fermentation system of edible and medicinal fungi as claimed in claims 1 and 4, wherein: the inner pipe body (702) is connected with the tail gas monitoring module (5) through a third branch pipe and a gas pump.
6. The automatic monitoring and control system for the liquid culture fermentation system of edible and medicinal fungi as claimed in claim 1, wherein: and a defoaming electrode is arranged above the inside of the fermentation tank (1), and the height of the bottommost part of the defoaming electrode is not more than the height of the topmost part of the upper pipe body (708).
7. The automatic monitoring and control system for the liquid culture fermentation system of edible and medicinal fungi as claimed in claim 1, wherein: the number of the foaming-defoaming combined devices (7) is not less than two and the foaming-defoaming combined devices are arranged in the fermentation tank (1) in a ring-shaped matrix.
8. The automatic monitoring and control system for liquid culture fermentation system of edible and medicinal fungi as claimed in claims 1 and 7, characterized in that: the inner wall and the outer wall of the outer pipe body (701) and the inner pipe body (702) are respectively provided with a reed (709) capable of freely vibrating, and the reed (709) is provided with a plurality of micropores.
9. The automatic monitoring and control system for liquid culture fermentation system of edible and medicinal fungi as claimed in claims 1 and 7, characterized in that: the outer pipe body (701) is movably connected with the body of the fermentation tank (1), and the outer pipe body (701) is rotated to adjust the position of the outer micropore area (705) above the outer pipe body in the fermentation tank (1).
10. The automatic monitoring and control system for the liquid culture fermentation system of edible and medicinal fungi as claimed in claim 1, wherein: the density of micropores on the outer micropore area (705) below the outer tube body (701) is greater than that of micropores on the outer micropore area (705) above the outer tube body.
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