CN112858966B - Spin confocal magnetic detection system and method - Google Patents
Spin confocal magnetic detection system and method Download PDFInfo
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- CN112858966B CN112858966B CN202110067232.9A CN202110067232A CN112858966B CN 112858966 B CN112858966 B CN 112858966B CN 202110067232 A CN202110067232 A CN 202110067232A CN 112858966 B CN112858966 B CN 112858966B
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Abstract
The invention provides a spin-focusing magnetic detection system and a spin-focusing magnetic detection method, wherein an emission source of the detection system emits an initial light source signal, the initial light source signal is converted into a regulating light signal through a wave selection amplifying unit and a polarization regulating unit, a beam splitter divides the regulating light signal into a first light signal and a second light signal, the first light signal is focused on a magnetic sample through a first focusing unit, a sample container provides a non-uniform magnetic field, the magnetic sample generates an excitation light signal and a fluorescence signal, the excitation light signal and the fluorescence signal return to the beam splitter and are overlapped with the transmission direction of the second light signal, the excitation light signal, the fluorescence signal and the second light signal are focused and filtered through a second focusing unit, the polarization type conversion is carried out through a conversion unit, and the atomic reaction mechanism of the sample to be detected is obtained through a preprocessing unit. The invention can distinguish photons carrying sample information from noise scattered photons with low momentum, obtain the reaction mechanism of the sample to be detected, effectively reduce the noise interference of a non-focal plane, and improve the spatial resolution and the imaging quality.
Description
Technical Field
The invention relates to the technical field of spin confocal detection magnetism, in particular to a spin confocal detection magnetic sample and a method.
Background
In the field of microscopic detection, increasing the detection thickness of a sample, improving resolution and imaging quality have been targets pursued by scientists. The confocal microscope forms a point light source by scanning light beams through a grating pinhole, scans point by point on a focal plane of a fluorescence-labeled specimen, collects optical signals of points to a receiver through a detection pinhole, and forms an image on a computer monitoring screen after signal processing. Light emitted at the focal point of the objective focal plane can be well converged at the pinhole, and can be received by the detector through the pinhole. Light emitted from the upper and lower positions of the focal plane can generate light spots with large diameters at the positions of the pinholes, and only a small part of light can penetrate through the pinholes and be received by the detector by comparing the diameters of the pinholes. And as the distance from the focal plane of the objective lens is larger, the stray light generated by the sample has larger diffuse spot at the pinhole, and the energy capable of penetrating the pinhole is smaller (from 10% to 1%, slowly approaching 0%), so that the signal generated on the detector is smaller, and the influence is smaller. Just because confocal microscopy only images the focal plane of the sample, interference of diffracted light and scattered light is effectively avoided, so that the confocal microscope has higher resolution than a common microscope.
The conventional confocal microscope is used for optical signal imaging, a receiver is difficult to distinguish photons carrying important information and scattered noise photons for the detection of a magnetic sample, the two photons are identical in momentum space, a specific device for spin magnetic moment split-column energy level imaging confocal detection does not exist in the prior art, and the magnetic sample imaging confocal detection and the research on a target system mechanism and quantum nonlinear characteristics cannot be realized based on the specific device.
Disclosure of Invention
In order to solve the above problems, the present invention provides a magnetic system and method for spin confocal detection, which can transmit and distinguish noise scattered photons with low momentum compared with a conventional microscope, effectively reduce the interference of non-focal plane noise, and improve spatial resolution and imaging quality.
In order to achieve the purpose, the invention provides the following scheme:
a magnetic spin confocal detection system for detecting a magnetic sample to be detected, the magnetic spin confocal detection system comprising:
an emission source for emitting an initial light source signal;
the wave selection amplifying unit is arranged on a transmission light path of the initial light source signal and is used for obtaining a wave selection amplifying light signal according to the initial light source signal;
the polarization adjusting unit is arranged on a transmission light path of the wave selection amplifying optical signal and is used for adjusting the wave selection amplifying optical signal and focusing the wave selection amplifying optical signal into a beam of light to obtain an adjusting optical signal;
the beam splitter is arranged on the transmission optical path of the adjusting optical signal and is used for splitting the adjusting optical signal into a first optical signal transmitted along the transmission optical path of the adjusting optical signal and a second optical signal vertical to the transmission direction of the first optical signal;
the first focusing unit is arranged on a transmission light path of the first optical signal and used for focusing the first optical signal on the magnetic sample to be detected; the first optical signal irradiates the magnetic sample to be detected, and the magnetic sample to be detected is excited by the first optical signal to generate an excitation light signal and a fluorescence signal which carry sample information; the excitation light signal and the fluorescence signal return to the beam splitter in a direction opposite to a transmission optical path of the first light signal; the beam splitter is further used for deflecting the excitation light signals and the fluorescence signals returned to the beam splitter by 90 degrees so that the transmission directions of the excitation light signals and the fluorescence signals coincide with the transmission direction of the second light signals;
the magnetic sample container is used for containing a magnetic sample to be detected and providing a non-uniform magnetic field for the magnetic sample to be detected;
the second focusing unit is arranged on a transmission light path of the second optical signal and is used for obtaining a first filtered optical signal according to the exciting light signal at the beam splitter, obtaining a filtered fluorescent signal according to the fluorescent signal at the beam splitter and obtaining a second filtered optical signal according to the second optical signal;
the conversion unit is arranged on a transmission light path of the first filtered light signal, the filtered fluorescence signal and the second filtered light signal, and is used for converting and analyzing the polarized light type in the first filtered light signal to obtain a first detection light signal, converting and analyzing the polarized light type in the filtered fluorescence signal to obtain a detection fluorescence signal, and converting and analyzing the polarized light type in the second filtered light signal to obtain a second detection light signal;
and the preprocessing unit is arranged on a transmission light path of the first detection light signal, the detection fluorescent signal and the second detection light signal and is used for obtaining an atomic reaction mechanism of the magnetic sample to be detected according to the first detection light signal, the detection fluorescent signal and the second detection light signal.
Optionally, the wave selection amplifying unit includes:
the first filter is positioned on a transmission light path of the initial light source signal and is used for filtering the initial light source signal to obtain a filtered light signal;
the first half-wave plate is positioned on a transmission light path of the filtering light signal and used for polarizing the filtering light signal to obtain a polarized light signal;
the electro-optical modulator is positioned on a transmission light path of the polarized light signal and is used for carrying out pulse modulation on the polarized light signal to obtain a pulse light signal;
the first beam expander and the second beam expander are positioned on a transmission light path of the pulse light signals and used for collimating and expanding the pulse light signals to obtain wave-selecting amplified light signals.
Optionally, the polarization adjustment unit includes:
the spatial polaroid is positioned on a transmission light path of the wave selection amplified optical signal and is used for screening the wave selection amplified optical signal with set wavelength to obtain a screened optical signal;
the polarization analyzer is positioned on a transmission light path of the screening light signal and is used for monitoring and adjusting the screening light signal according to a set angle, intensity, phase and pulse width to obtain an adjusted light signal;
and the cylindrical mirror is positioned on the transmission light path of the adjusting light signal and is used for carrying out line focusing on the adjusting light signal to obtain an adjusting light signal.
Optionally, the first focusing unit further comprises:
the first lens is positioned on a transmission light path of the first optical signal and used for carrying out beam collimation and focusing on the first optical signal;
the first excitation galvanometer is positioned on a transmission light path of the first optical signal and used for deflecting the first optical signal by 90 degrees to obtain a deflected first optical signal;
the scanning mirror is positioned on a transmission light path of the deflected first optical signal and is used for performing rapid line scanning on the deflected first optical signal to obtain a scanned first optical signal;
the tube mirror is positioned on a transmission light path of the scanned first optical signal;
and the objective lens is positioned on the transmission optical path of the scanned first optical signal.
Optionally, the second focusing unit further comprises:
the second lens is positioned on the transmission optical path of the second optical signal;
a third lens, located on the transmission optical path of the second optical signal, for enlarging the beam diameter in combination with the second lens;
the slit is positioned on a transmission light path of the second optical signal and is used for carrying out stray light filtration on the exciting light signal to obtain a first filtered optical signal, carrying out stray light filtration on the fluorescent signal to obtain the filtered fluorescent signal and carrying out stray light filtration on the second optical signal to obtain a second filtered optical signal;
a fourth lens, located on a transmission light path of the first filtered light signal, the filtered fluorescent signal, and the second filtered light signal, for collimating and focusing the first filtered light signal, the filtered fluorescent signal, and the second filtered light signal;
the second excitation galvanometer is positioned on a transmission light path of the first filtered light signal, the filtered fluorescent signal and the second filtered light signal and is used for deflecting the first filtered light signal by 90 degrees to obtain a deflected first filtered light signal, deflecting the filtered fluorescent signal by 90 degrees to obtain a deflected filtered fluorescent signal and deflecting the second filtered light signal by 90 degrees to obtain a deflected second filtered light signal;
and the fifth lens is positioned on a transmission light path of the deflected first filtered light signal, the deflected filtered fluorescent signal and the deflected second filtered light signal and is used for collimating and focusing the deflected first filtered light signal, the deflected filtered fluorescent signal and the deflected second filtered light signal.
Optionally, the second focusing unit further comprises:
and the second filter is positioned between the fourth lens and the second excitation galvanometer and is used for filtering the first filtered light signal, the filtered fluorescent signal and the second filtered light signal.
Optionally, the conversion unit includes:
the quarter-wave plate is positioned on the light path of the deflected first filtered light signal, the deflected filtered fluorescent signal and the second filtered light signal and positioned between the fifth lens and the preprocessing unit, and is used for converting the polarized light type in the first filtered light signal to obtain a first converted light signal, converting the polarized light type in the deflected filtered fluorescent signal to obtain a converted fluorescent signal and converting the polarized light type in the second filtered light signal to obtain a second converted light signal;
and the second half-wave plate is positioned on the light paths of the first conversion light signal, the conversion fluorescent signal and the second conversion light signal and positioned between the quarter-wave plate and the preprocessing unit, and the second half-wave plate is used for analyzing the first conversion light signal to obtain a first detection light signal, analyzing the conversion fluorescent signal to obtain a detection fluorescent signal and analyzing the second conversion light signal to obtain a second detection light signal.
Optionally, the pre-processing unit comprises:
and the spectrometer is used for obtaining a composition spectrogram of the magnetic sample to be detected according to the first detection optical signal, the detection fluorescent signal and the second detection optical signal, and the composition spectrogram is used for representing an atomic reaction mechanism of the magnetic sample to be detected.
Optionally, the pre-processing unit comprises:
and the detector is used for obtaining a particle trajectory diagram of the magnetic sample to be detected according to the first detection light signal, the detection fluorescence signal and the second detection light signal, and the particle trajectory diagram is used for representing an atomic reaction mechanism of the magnetic sample to be detected.
In order to achieve the above purpose, the invention also provides the following scheme:
a magnetic method for magnetic detection by spin confocal imaging is applied to the magnetic system for magnetic detection by spin confocal imaging, and comprises the following steps:
transmitting an initial light source signal through a transmission source;
receiving the initial light source signal through a wave selection amplifying unit, and obtaining a wave selection amplifying signal according to the initial light source signal;
adjusting the wave selection amplification signal through a polarization adjusting unit, and focusing the wave selection amplification signal into a beam of light to obtain an adjusted light signal;
dividing the adjusting optical signal into a first optical signal transmitted along a transmission optical path of the adjusting optical signal and a second optical signal vertical to the transmission direction of the first optical signal by a beam splitter;
focusing the first optical signal on the magnetic sample to be detected through a first focusing unit; the first optical signal irradiates the magnetic sample to be detected, and the magnetic sample to be detected is excited by the first optical signal to generate an excitation optical signal and a fluorescence signal which carry sample information; the excitation light signal and the fluorescence signal return to the beam splitter along the direction opposite to the transmission light path of the first light signal; the beam splitter is further used for deflecting the excitation light signals and the fluorescence signals returned to the beam splitter by 90 degrees so that the transmission directions of the excitation light signals and the fluorescence signals coincide with the transmission direction of the second light signals;
the method comprises the following steps of (1) containing a magnetic sample to be detected through a magnetic sample container and providing a non-uniform magnetic field for the magnetic sample to be detected;
obtaining a first filtered light signal according to the excitation light signal at the beam splitter, obtaining a filtered fluorescence signal according to the fluorescence signal, and obtaining a second filtered light signal according to the second light signal by a second focusing unit;
converting the polarized light type in the first filtered light signal and analyzing the polarized light type to obtain a first detection light signal, converting the polarized light type in the filtered fluorescence signal and analyzing the polarized light type to obtain a detection fluorescence signal, and converting the polarized light type in the second filtered light signal and analyzing the polarized light type to obtain a second detection light signal by a conversion unit;
and obtaining the atomic reaction mechanism of the magnetic sample to be detected through a preprocessing unit according to the first detection optical signal, the detection fluorescent signal and the second detection optical signal.
According to the specific embodiment provided by the invention, the invention discloses the following technical effects:
the invention relates to a spin confocal magnetic detection system and a method, which are used for detecting a magnetic sample to be detected; the emission source emits an initial light source signal, the initial light source signal forms an adjusting light signal after passing through the wave selection amplifying unit and the polarization adjusting unit, the beam splitter divides the adjusting light signal into a first light signal and a second light signal which is vertical to the transmission direction of the first light signal, the first focusing unit focuses the first light signal on a magnetic sample to be detected, the magnetic sample to be detected is excited by the first light signal to generate an excitation light signal and a fluorescence signal which carry sample information, the excitation light signal and the fluorescence signal return to the beam splitter along the direction opposite to the transmission light path of the first light signal, the beam splitter enables the transmission directions of the excitation light signal and the fluorescence signal to coincide with the transmission direction of the second light signal, the second focusing unit obtains a first filtering light signal according to the excitation light signal at the beam splitter, obtains a filtering fluorescence signal according to the fluorescence signal and obtains a second filtering light signal according to the second light signal, the conversion unit converts and analyzes the polarized light type in the first filtered light signal to obtain a first detection light signal, converts and analyzes the polarized light type in the filtered fluorescence signal to obtain a detection fluorescence signal, converts and analyzes the polarized light type in the second filtered light signal to obtain a second detection light signal, and the preprocessing unit obtains the atomic reaction mechanism of the magnetic sample to be detected according to the first detection light signal, the detection fluorescence signal and the second detection light signal. The invention can directly detect the photons carrying the sample information and the focusing depth tested in the sample to obtain the reaction mechanism of the sample to be tested, and can distinguish the photons carrying the sample information from noise scattered photons with low momentum; the degenerate energy levels of atoms and molecules are effectively eliminated through the non-uniform magnetic field, the interference of non-focal plane noise is reduced through a confocal system, and the spatial resolution and the imaging quality are improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a schematic diagram of a spin-confocal magnetic detection system according to the present invention;
FIG. 2 is a schematic diagram of a sample container of the magnetic spin-confocal detection system according to the present invention;
FIG. 3 is a flow chart of the method for magnetic detection by spin-confocal measurement according to the present invention.
Description of the symbols:
1-a source of emission; 2-a wave selection amplifying unit, 21-a first filter, 22-a first half wave plate, 23-an electro-optic modulator, 24-a first beam expander and 25-a second beam expander;
3-polarization adjusting unit, 31-space polaroid, 32-polarization analyzer, 33-cylindrical mirror;
4-a beam splitter; 5-a first focusing unit, 51-a first lens, 52-a first excitation galvanometer, 53-a scanning mirror, 54-a tube mirror, 55-an objective lens;
6-magnetic sample container; 7-second focusing unit, 71-second lens, 72-third lens, 73-slit, 74-fourth lens, 75-second filter, 76-second excitation galvanometer, 77-fifth lens;
8-conversion unit, 81-quarter wave plate, 82-second half wave plate, 9-preprocessing unit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide a spin-focusing confocal magnetic detection system, which directly detects photons carrying sample information and the focusing depth tested on a magnetic sample, obtains the reaction mechanism of the magnetic sample to be tested, distinguishes the photons carrying the sample information from noise scattered photons, effectively reduces the interference of non-focal plane noise, and improves the spatial resolution and the imaging quality.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
As shown in fig. 1, the magnetic system for spin confocal detection of the present invention includes: the device comprises an emission source 1, a wave selection amplifying unit 2, a polarization adjusting unit 3, a beam splitter 4, a first focusing unit 5, a magnetic sample container 6, a second focusing unit 7, a conversion unit 8 and a preprocessing unit 9.
In particular, the emission source 1 is used to emit an initial light source signal.
The wave selection amplifying unit 2 is arranged on a transmission light path of the initial light source signal and is used for obtaining a wave selection amplifying light signal according to the initial light source signal.
The polarization adjusting unit 3 is arranged on a transmission light path of the wave selection amplifying optical signal and is used for adjusting the wave selection amplifying optical signal and focusing the wave selection amplifying optical signal into a beam of light to obtain an adjusting optical signal.
The beam splitter 4 is disposed on the transmission optical path of the conditioning optical signal, and is configured to split the conditioning optical signal into a first optical signal transmitted along the transmission optical path of the conditioning optical signal and a second optical signal perpendicular to the transmission direction of the first optical signal.
The first focusing unit 5 is disposed on a transmission optical path of the first optical signal, and is configured to focus the first optical signal on the magnetic sample to be measured. The first optical signal irradiates the magnetic sample to be detected, and the magnetic sample to be detected is excited by the first optical signal to generate an excitation optical signal and a fluorescence signal which carry sample information. The excitation light signal and the fluorescence signal are returned to the beam splitter 4 in the opposite direction to the transmission optical path of the first light signal. The beam splitter 4 is further configured to deflect the excitation light signal and the fluorescence signal returned to the beam splitter 4 by 90 degrees so that the transmission directions of the excitation light signal and the fluorescence signal coincide with the transmission direction of the second light signal.
The magnetic sample container 6 is used for containing a magnetic sample to be detected and providing a non-uniform magnetic field for the magnetic sample to be detected.
The second focusing unit 7 is disposed on a transmission light path of the second optical signal, and is configured to obtain a first filtered optical signal according to the excitation optical signal at the beam splitter, obtain a filtered fluorescence signal according to the fluorescence signal, and obtain a second filtered optical signal according to the second optical signal.
The conversion unit 8 is disposed on a transmission light path of the first filtered light signal, the filtered fluorescence signal, and the second filtered light signal, and configured to convert and analyze a polarized light type in the first filtered light signal to obtain a first detection light signal, convert and analyze a polarized light type in the filtered fluorescence signal to obtain a detection fluorescence signal, and convert and analyze a polarized light type in the second filtered light signal to obtain a second detection light signal.
The preprocessing unit 9 is disposed on a transmission light path of the first detection light signal, the detection fluorescence signal, and the second detection light signal, and configured to obtain an atomic reaction mechanism of the magnetic sample to be detected according to the first detection light signal, the detection fluorescence signal, and the second detection light signal.
Preferably, the emission source 1 may be a laser transmitter with good coherence of light sources, such as a free electron laser, a higher harmonic generator, and an optical comb trigger.
Further, as shown in fig. 1, the wave selection amplifying unit 2 includes: a first filter 21, a half-wave plate 22, an electro-optical modulator 23, a first beam expander lens 24 and a second beam expander lens 25.
The first filter 21 is located on a transmission optical path of the initial light source signal, and is configured to filter the initial light source signal to obtain a filtered light signal.
The first half-wave plate 22 is located on a transmission light path of the filtered light signal, and is configured to polarize the filtered light signal to obtain a polarized light signal.
The electro-optical modulator 23 is located on a transmission optical path of the polarized light signal, and is configured to perform pulse modulation on the polarized light signal to obtain a pulse light signal.
The first beam expander 24 and the second beam expander 25 are located on a transmission light path of the pulse light signals, and are used for collimating and expanding the pulse light signals to obtain wave-selecting amplified light signals.
Further, the polarization adjustment unit 3 includes: a spatial polarizer 31, a polarization analyzer 32, and a cylindrical mirror 33.
The spatial polarizer 31 is located on a transmission light path of the wave-selective amplified optical signal, and is configured to screen the wave-selective amplified optical signal with a set wavelength to obtain a screened optical signal.
The polarization analyzer 32 is located on the transmission light path of the screening light signal, and is configured to monitor and adjust the screening light signal according to a set angle, intensity, phase, and pulse width to obtain an adjusted light signal.
The cylindrical mirror 33 is located on the transmission optical path of the adjusting optical signal, and is used for performing line focusing on the adjusting optical signal to obtain an adjusting optical signal.
Further, the first focusing unit 5 includes: a first lens 51, a first excitation galvanometer 52, a scanning mirror 53, a tube mirror 54, and an objective lens 55.
The first lens 51 is located on a transmission optical path of the first optical signal, and is configured to perform beam collimation and focusing on the first optical signal.
The first excitation galvanometer 52 is located on a transmission optical path of the first optical signal and is configured to deflect the first optical signal by 90 degrees to obtain a deflected first optical signal.
The scanning mirror 53 is located on a transmission optical path of the deflected first optical signal, and is configured to perform fast line scanning on the deflected first optical signal to obtain a scanned first optical signal.
The tube mirror 54 is located on the transmission optical path of the scanned first optical signal.
The objective lens 55 is located on the transmission optical path of the scanned first optical signal.
In order to obtain more accurate detection results, the second focusing unit 7 further includes: a second lens 71, a third lens 72, a slit 73, a fourth lens 74, a second galvanometer 76, and a fifth lens 77.
The second lens 71 is located on a transmission optical path of the second optical signal.
The third lens 72 is located on a transmission optical path of the second optical signal, and is combined with the second lens 71 for enlarging a beam diameter.
The slit 73 is located on a transmission light path of the second optical signal, and is configured to perform stray light filtering on the excitation optical signal to obtain a first filtered optical signal, perform stray light filtering on the fluorescence signal to obtain the filtered fluorescence signal, and perform stray light filtering on the second optical signal to obtain a second filtered optical signal. The size of the slit 73 determines the depth, resolution and imaging quality with which the magnetic sample to be examined can be detected.
The fourth lens 74 is disposed on the transmission light path of the first filtered light signal, the filtered fluorescent signal, and the second filtered light signal, and is configured to collimate and focus the first filtered light signal, the filtered fluorescent signal, and the second filtered light signal.
The second excitation galvanometer 76 is located on a transmission light path of the first filtered light signal, the filtered fluorescent signal and the second filtered light signal, and is configured to perform 90-degree deflection on the first filtered light signal to obtain a deflected first filtered light signal, perform 90-degree deflection on the filtered fluorescent signal to obtain a deflected filtered fluorescent signal, and perform 90-degree deflection on the second filtered light signal to obtain a deflected second filtered light signal.
The fifth lens 77 is located on a transmission light path of the deflected first filtered light signal, the deflected filtered fluorescent signal, and the deflected second filtered light signal, and is configured to collimate and focus the deflected first filtered light signal, the deflected filtered fluorescent signal, and the deflected second filtered light signal.
In order to further optimize the above system, the second focusing unit 7 further comprises: a second filter 75.
The second filter 75 is disposed between the fourth lens 74 and the second excitation galvanometer 76, and is used for filtering the first filtered light signal, the filtered fluorescence signal, and the second filtered light signal.
In order to improve the accuracy of the detection result, the conversion unit 8 includes: a quarter-wave plate 81 and a second half-wave plate 82.
The quarter-wave plate 81 is located on the light path of the deflected first filtered light signal, the deflected filtered fluorescent signal and the second filtered light signal and located between the fifth lens 77 and the preprocessing unit 9, and the quarter-wave plate 81 is configured to convert the polarized light type in the first filtered light signal to obtain a first converted light signal, convert the polarized light type in the deflected filtered fluorescent signal to obtain a converted fluorescent signal and convert the polarized light type in the second filtered light signal to obtain a second converted light signal. Taking the first filtered optical signal as an example, after the first filtered optical signal passes through the quarter-wave plate 81, linearly polarized light contained in the first filtered optical signal is converted into circularly polarized light, circularly polarized light is converted into linearly polarized light, and finally, a first converted signal is obtained. The purpose of switching the type of polarized light is to distinguish the energy level transitions of the atoms and thus whether the atoms are left-handed or right-handed.
The second half-wave plate 82 is located on the light path of the first converted light signal, the converted fluorescent signal and the second converted light signal and located between the quarter-wave plate 81 and the preprocessing unit 9, and the second half-wave plate 82 is used for performing polarization detection on the first converted light signal to obtain a first detected light signal, performing polarization detection on the converted fluorescent signal to obtain a detected fluorescent signal and performing polarization detection on the second converted light signal to obtain a second detected light signal.
Example 1:
in order to obtain a spectrogram of an optical signal, in embodiment 1 of the present invention, the preprocessing unit includes: spectrometer (not shown).
The spectrometer is used for obtaining a composition spectrogram of the sample to be detected according to the first detection optical signal, the detection fluorescent signal and the second detection optical signal, and the composition spectrogram is used for representing an atomic reaction mechanism of the sample to be detected. The second detection light signal contains photon information of the initial light source signal and can be used as reference light.
Example 2:
in order to obtain a traveling trace pattern of particles, in embodiment 2 of the present invention, the preprocessing unit includes: a detector (not shown).
The detector is used for obtaining a particle trajectory graph of the sample to be detected according to the first detection optical signal, the detection fluorescent signal and the second detection optical signal, and the particle trajectory graph is used for representing an atomic reaction mechanism of the sample to be detected. Wherein the second detection light signal can be used as reference light.
As shown in fig. 3, to achieve the above object, the present invention further provides the following solutions:
a magnetic method for spin confocal magnetic detection is applied to the magnetic system for spin confocal magnetic detection, and comprises the following steps:
an initial source signal is emitted by a source.
And receiving the initial light source signal through a wave selection amplifying unit, and obtaining a wave selection amplifying signal according to the initial light source signal.
And adjusting the wave selection amplification signal through a polarization adjusting unit, and focusing the wave selection amplification signal into a beam of light to obtain an adjusted light signal.
The modulation optical signal is split by a beam splitter into a first optical signal transmitted along a transmission optical path of the modulation optical signal and a second optical signal perpendicular to a transmission direction of the first optical signal.
And focusing the first optical signal on the magnetic sample to be detected through a first focusing unit. The first optical signal irradiates the magnetic sample to be detected, and the magnetic sample to be detected is excited by the first optical signal to generate an excitation light signal and a fluorescence signal which carry sample information. The excitation light signal and the fluorescence signal return to the beam splitter along a direction opposite to a transmission optical path of the first light signal. The beam splitter is further used for deflecting the excitation light signals and the fluorescence signals returned to the beam splitter by 90 degrees so that the transmission directions of the excitation light signals and the fluorescence signals coincide with the transmission direction of the second light signals.
The magnetic sample container is used for containing a magnetic sample to be detected and providing a non-uniform magnetic field for the magnetic sample to be detected.
And obtaining a first filtered light signal according to the exciting light signal at the beam splitter through a second focusing unit, obtaining a filtered fluorescence signal according to the fluorescence signal, and obtaining a second filtered light signal according to the second light signal.
The conversion unit converts the polarized light type in the first filtered light signal and performs polarization detection to obtain a first detection light signal, converts the polarized light type in the filtered fluorescence signal and performs polarization detection to obtain a detection fluorescence signal, and converts the polarized light type in the second filtered light signal and performs polarization detection to obtain a second detection light signal.
And obtaining the atomic reaction mechanism of the magnetic sample to be detected according to the first detection optical signal, the detection fluorescent signal and the second detection optical signal by a preprocessing unit.
The principle of the invention is as follows: the inside of the magnetic sample container is a non-uniform magnetic field, the non-uniform magnetic field causes the electron spin in the sample atom molecules to be selectively oriented and the interaction of the spin and the orbit splits the energy level, the energy level splitting interval is larger and the splitting interval is related to the magnetic field intensity,
Δ＝±S z μ B b (r), Delta is the energy level splitting width of the electron under the action of external magnetic field, S z Is spin, μ B Is a Bohr magneton, B is a non-uniform magnetic induction, B is a function of a spatial coordinate r.
The energy level splitting can see different internal structures of atoms, and the reaction mechanism of atoms is obtained by detection so as to see the information of the internal structures.
As shown in fig. 2, an embodiment of the present invention provides a schematic view of a magnetic sample container. Wherein, inhomogeneous magnetic field generator 1601 is a magnet or an ac coil, inhomogeneous magnetic field generator 1603 is a magnet or an ac coil, and magnetic sample container console 1605 is used to adjust the size of inhomogeneous magnetic field in magnetic sample container power 1604 and sample stage 1602 space.
Magnetic field gradient
f (r) is the modulation function.
When the modulation function f (r) is 0, the applied magnetic field is a uniform magnetic field, and it is difficult to distinguish degenerate levels, and states cannot be distinguished.
When the modulation function f (r) is 0, the external magnetic field is a non-uniform magnetic field, so that degenerate energy levels are completely separated, the layers are clear, and accurate spatial positioning can be realized.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The method disclosed by the embodiment corresponds to the system disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the description of the method part.
The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and the core concept of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (9)
1. A confocal spin detection magnetic system for detecting a magnetic sample to be detected, the confocal spin detection magnetic system comprising:
an emission source for emitting an initial light source signal;
the wave selection amplifying unit is arranged on a transmission light path of the initial light source signal and is used for obtaining a wave selection amplifying light signal according to the initial light source signal;
the polarization adjusting unit is arranged on a transmission light path of the wave selection amplifying optical signal and is used for adjusting the wave selection amplifying optical signal and focusing the wave selection amplifying optical signal into a beam of light to obtain an adjusting optical signal;
the beam splitter is arranged on the transmission optical path of the adjusting optical signal and is used for splitting the adjusting optical signal into a first optical signal transmitted along the transmission optical path of the adjusting optical signal and a second optical signal vertical to the transmission direction of the first optical signal;
the first focusing unit is arranged on a transmission light path of the first optical signal and used for focusing the first optical signal on the magnetic sample to be detected; the first optical signal irradiates the magnetic sample to be detected, and the magnetic sample to be detected is excited by the first optical signal to generate an excitation light signal and a fluorescence signal which carry sample information; the excitation light signal and the fluorescence signal return to the beam splitter in a direction opposite to a transmission optical path of the first light signal; the beam splitter is further used for deflecting the excitation light signals and the fluorescence signals returned to the beam splitter by 90 degrees so that the transmission directions of the excitation light signals and the fluorescence signals coincide with the transmission direction of the second light signals;
the magnetic sample container is used for containing a magnetic sample to be detected and providing a non-uniform magnetic field for the magnetic sample to be detected;
the second focusing unit is arranged on a transmission light path of the second optical signal and is used for obtaining a first filtered optical signal according to the exciting light signal at the beam splitter, obtaining a filtered fluorescent signal according to the fluorescent signal at the beam splitter and obtaining a second filtered optical signal according to the second optical signal;
the conversion unit is arranged on a transmission light path of the first filtered light signal, the filtered fluorescence signal and the second filtered light signal, and is used for converting and analyzing the polarized light type in the first filtered light signal to obtain a first detection light signal, converting and analyzing the polarized light type in the filtered fluorescence signal to obtain a detection fluorescence signal, and converting and analyzing the polarized light type in the second filtered light signal to obtain a second detection light signal;
the preprocessing unit is arranged on a transmission light path of the first detection light signal, the detection fluorescent signal and the second detection light signal and is used for obtaining an atomic reaction mechanism of the magnetic sample to be detected according to the first detection light signal, the detection fluorescent signal and the second detection light signal;
the conversion unit includes:
the quarter-wave plate is positioned on the light path of the deflected first filtered light signal, the deflected filtered fluorescent signal and the second filtered light signal and positioned between the fifth lens and the preprocessing unit, and is used for converting the polarized light type in the first filtered light signal to obtain a first converted light signal, converting the polarized light type in the deflected filtered fluorescent signal to obtain a converted fluorescent signal and converting the polarized light type in the second filtered light signal to obtain a second converted light signal;
the second half-wave plate is positioned on the light path of the first conversion light signal, the conversion fluorescent signal and the second conversion light signal and positioned between the quarter-wave plate and the preprocessing unit, and the second half-wave plate is used for analyzing the first conversion light signal to obtain a first detection light signal, analyzing the conversion fluorescent signal to obtain a detection fluorescent signal and analyzing the second conversion light signal to obtain a second detection light signal;
the magnetic sample container comprises a non-uniform magnetic field generator, a magnetic sample container control console, a sample stage and a power supply; the non-uniform magnetic field generator is a magnet or an alternating current coil; the magnetic sample container console is used to adjust the power supply size and the size of the non-uniform magnetic field in the sample stage space.
2. The confocal spin detection magnetic system according to claim 1, wherein the wave selection amplifying unit comprises:
the first filter is positioned on a transmission light path of the initial light source signal and is used for filtering the initial light source signal to obtain a filtered light signal;
the first half-wave plate is positioned on a transmission light path of the filtering light signal and used for polarizing the filtering light signal to obtain a polarized light signal;
the electro-optical modulator is positioned on a transmission light path of the polarized light signal and is used for carrying out pulse modulation on the polarized light signal to obtain a pulse light signal;
the first beam expander and the second beam expander are positioned on a transmission light path of the pulse light signals and used for collimating and expanding the pulse light signals to obtain wave-selecting amplified light signals.
3. The confocal spin detection magnetic system according to claim 1, wherein the polarization adjustment unit comprises:
the spatial polarizer is positioned on a transmission light path of the wave selection amplified optical signal and is used for screening the wave selection amplified optical signal with a set wavelength to obtain a screened optical signal;
the polarization analyzer is positioned on a transmission light path of the screening light signal and is used for monitoring and adjusting the screening light signal according to a set angle, intensity, phase and pulse width to obtain an adjusted light signal;
and the cylindrical mirror is positioned on the transmission light path of the adjusting light signal and is used for carrying out line focusing on the adjusting light signal to obtain an adjusting light signal.
4. The magnetic spin confocal detection system of claim 1, wherein the first focusing unit further comprises:
the first lens is positioned on a transmission light path of the first optical signal and used for carrying out beam collimation and focusing on the first optical signal;
the first excitation galvanometer is positioned on a transmission light path of the first optical signal and used for deflecting the first optical signal by 90 degrees to obtain a deflected first optical signal;
the scanning mirror is positioned on a transmission light path of the deflected first optical signal and is used for performing rapid line scanning on the deflected first optical signal to obtain a scanned first optical signal;
the tube mirror is positioned on a transmission light path of the scanned first optical signal;
and the objective lens is positioned on the transmission optical path of the scanned first optical signal.
5. The spin confocal detection magnetic system of claim 1, wherein the second focusing unit further comprises:
the second lens is positioned on the transmission optical path of the second optical signal;
a third lens, located on a transmission optical path of the second optical signal, combined with the second lens for enlarging a beam diameter;
the slit is positioned on a transmission light path of the second optical signal and is used for carrying out stray light filtration on the exciting light signal to obtain a first filtered optical signal, carrying out stray light filtration on the fluorescent signal to obtain the filtered fluorescent signal and carrying out stray light filtration on the second optical signal to obtain a second filtered optical signal;
a fourth lens, located on a transmission light path of the first filtered light signal, the filtered fluorescent signal and the second filtered light signal, for collimating and focusing the first filtered light signal, the filtered fluorescent signal and the second filtered light signal;
the second excitation galvanometer is positioned on a transmission light path of the first filtered light signal, the filtered fluorescent signal and the second filtered light signal and is used for deflecting the first filtered light signal by 90 degrees to obtain a deflected first filtered light signal, deflecting the filtered fluorescent signal by 90 degrees to obtain a deflected filtered fluorescent signal and deflecting the second filtered light signal by 90 degrees to obtain a deflected second filtered light signal;
and the fifth lens is positioned on a transmission light path of the deflected first filtered light signal, the deflected filtered fluorescent signal and the deflected second filtered light signal and is used for collimating and focusing the deflected first filtered light signal, the deflected filtered fluorescent signal and the deflected second filtered light signal.
6. The spin confocal detection magnetic system of claim 5, wherein the second focusing unit further comprises:
and the second filter is positioned between the fourth lens and the second excitation galvanometer and is used for filtering the first filtered light signal, the filtered fluorescent signal and the second filtered light signal.
7. The magnetic spin confocal detection system of claim 1, wherein the preprocessing unit comprises:
and the spectrometer is used for obtaining a composition spectrogram of the magnetic sample to be detected according to the first detection optical signal, the detection fluorescent signal and the second detection optical signal, and the composition spectrogram is used for representing an atomic reaction mechanism of the magnetic sample to be detected.
8. The confocal spin detection magnetic system of claim 1, wherein the preprocessing unit comprises:
and the detector is used for obtaining a particle trajectory diagram of the magnetic sample to be detected according to the first detection optical signal, the detection fluorescent signal and the second detection optical signal, and the particle trajectory diagram is used for representing an atomic reaction mechanism of the magnetic sample to be detected.
9. A spin confocal magnetic detection method applied to the spin confocal magnetic detection system according to any one of claims 1 to 8, the magnetic detection method comprising:
transmitting an initial light source signal through a transmission source;
receiving the initial light source signal through a wave selection amplifying unit, and obtaining a wave selection amplifying signal according to the initial light source signal;
adjusting the wave selection amplification signal through a polarization adjusting unit, and focusing the wave selection amplification signal into a beam of light to obtain an adjusting light signal;
dividing the adjusting optical signal into a first optical signal transmitted along a transmission optical path of the adjusting optical signal and a second optical signal vertical to the transmission direction of the first optical signal by a beam splitter;
focusing the first optical signal on the magnetic sample to be detected through a first focusing unit; the first optical signal irradiates the magnetic sample to be detected, and the magnetic sample to be detected is excited by the first optical signal to generate an excitation optical signal and a fluorescence signal which carry sample information; the excitation light signal and the fluorescence signal return to the beam splitter along the direction opposite to the transmission light path of the first light signal; the beam splitter is further used for deflecting the excitation light signals and the fluorescence signals returned to the beam splitter by 90 degrees so that the transmission directions of the excitation light signals and the fluorescence signals coincide with the transmission direction of the second light signals;
the method comprises the following steps of (1) containing a magnetic sample to be detected through a magnetic sample container and providing a non-uniform magnetic field for the magnetic sample to be detected;
obtaining a first filtered light signal according to the excitation light signal at the beam splitter, obtaining a filtered fluorescence signal according to the fluorescence signal, and obtaining a second filtered light signal according to the second light signal by a second focusing unit;
converting the polarized light type in the first filtered light signal and analyzing the polarized light type to obtain a first detection light signal, converting the polarized light type in the filtered fluorescence signal and analyzing the polarized light type to obtain a detection fluorescence signal, and converting the polarized light type in the second filtered light signal and analyzing the polarized light type to obtain a second detection light signal by a conversion unit;
obtaining an atomic reaction mechanism of the magnetic sample to be detected according to the first detection optical signal, the detection fluorescent signal and the second detection optical signal through a preprocessing unit;
the conversion unit includes:
the quarter-wave plate is positioned on the light path of the deflected first filtered light signal, the deflected filtered fluorescent signal and the second filtered light signal and positioned between the fifth lens and the preprocessing unit, and is used for converting the polarized light type in the first filtered light signal to obtain a first converted light signal, converting the polarized light type in the deflected filtered fluorescent signal to obtain a converted fluorescent signal and converting the polarized light type in the second filtered light signal to obtain a second converted light signal;
the second half-wave plate is positioned on the light path of the first conversion light signal, the conversion fluorescent signal and the second conversion light signal and positioned between the quarter-wave plate and the preprocessing unit, and the second half-wave plate is used for analyzing the first conversion light signal to obtain a first detection light signal, analyzing the conversion fluorescent signal to obtain a detection fluorescent signal and analyzing the second conversion light signal to obtain a second detection light signal;
the magnetic sample container comprises a non-uniform magnetic field generator, a magnetic sample container control platform, a sample platform and a power supply; the non-uniform magnetic field generator is a magnet or an alternating current coil; the magnetic sample container console is used to adjust the power supply size and the size of the non-uniform magnetic field in the sample stage space.
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