CN112858019A - Test device and test method for repairing air pressure fracture by MICP technology - Google Patents

Test device and test method for repairing air pressure fracture by MICP technology Download PDF

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CN112858019A
CN112858019A CN202110029017.XA CN202110029017A CN112858019A CN 112858019 A CN112858019 A CN 112858019A CN 202110029017 A CN202110029017 A CN 202110029017A CN 112858019 A CN112858019 A CN 112858019A
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test
ball valve
pressure
way ball
air pressure
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CN112858019B (en
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许龙
鲁绪江
杨翔宇
黄艳凤
刘侃侃
查甫生
储诚富
朱方华
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Hefei University of Technology
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    • G01N3/00—Investigating strength properties of solid materials by application of mechanical stress
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    • G01N3/12—Pressure testing
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    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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Abstract

The invention provides a test device and a test method for repairing an air pressure fracture by a MICP technology, belongs to the technical field of geotechnical engineering, and can be used for air pressure fracture, microorganism grouting and repairing effect evaluation. The main body device comprises a test box main body, a sample layer, a coarse sand layer, a permeable stone, a grid measuring screen, a test box door, a sealing gasket, a through hole B, a through hole A and the like. In the test process, the development and development conditions of soil body shallow layer cracks and the evaluation of the microorganism repairing effect are monitored by controlling different dry densities, different injection pressures and different bacteria liquid concentrations of the sample layer and by the grid measuring screen and the volume of the sample layer drained water at certain intervals.

Description

Test device and test method for repairing air pressure fracture by MICP technology
Technical Field
The invention relates to the technical field of microorganism and geotechnical engineering, in particular to a test device and a method for repairing a pneumatic fracture by using a MICP technology.
Background
Since the innovation is open, the construction of various infrastructures in China is different day by day, such as urban rail transit, expressways, high-speed railways, ports, airports and the like, and the scale and the difficulty of the infrastructures are in international unprecedented levels, so that great challenges are provided for the foundation treatment technology. The existing better foundation treatment technology is an air pressure splitting vacuum preloading method for treating soft foundation, which is mainly applied to highways, high-speed railways and the like with extremely high deformation requirements, ensures the stability of embankment filling process, and ensures that the load does not exceed the bearing capacity of the embankment in the embankment construction process and the subsequent foundation consolidation process. But simultaneously, the crack generated by the air pressure splitting can generate hidden trouble to the stability of the engineering foundation, so that the crack needs to be repaired, and the engineering stability of the foundation is further improved.
The traditional repairing technology generally comprises the steps of grouting cement concrete, organic matters and the like for the crack or pressurizing. The relevant grouting raw materials can pollute the surrounding soil, and the stability in the pressurizing process can not be ensured. The MICP technology can solve the problems from various aspects. The microorganism induced calcium carbonate precipitation (MICP) technology refers to the technology that specific microorganisms (such as urease bacteria, denitrifying bacteria and the like) are injected into soil and provide rich calcium source and nitrogen source nutrients for the microorganisms, so that calcite type crystals are rapidly precipitated. Compared with the traditional method, the method has better environmental protection and durability.
Therefore, in order to solve the problem of clay foundation repair, a MICP technology repair air pressure fracture test device and a method are developed. The test device and the test method have important engineering practical significance and theoretical research value for expanding and applying the MICP technical range.
Disclosure of Invention
The invention aims to solve the problems and invents a testing device and a method for repairing an air pressure fracture by using the MICP technology.
The purpose of the invention can be realized by the following technical scheme.
The invention provides a test device for repairing an air pressure fracture by an MICP technology, which comprises a test box main body, a test box door and an auxiliary device, wherein the test box main body is provided with a test box opening; the cross section of the test box main body is rectangular, a permeable stone is embedded in the center of the bottom of the test box main body, a through hole B is reserved and communicated with the permeable stone, and through holes A are uniformly distributed in the top of the test box main body; the test box door is closed during testing, so that the inner cavity of the test box main body is sealed;
in the test box body, a sample layer consisting of a sample and a coarse sand layer consisting of coarse sand are respectively arranged from bottom to top, wherein the layer thickness of the sample layer is H1, the layer thickness of the coarse sand layer is H2, H is equal to or less than H which is the height of an inner cavity of the test box body and is H1+ H2;
the auxiliary device comprises a high-pressure gas cylinder, a pipeline, a pressure reducing valve A, a gas buffer tank, a pressure reducing valve B, a pressure sensor, a switch, a liquid pressure volume controller, a three-way ball valve A, an organic solution isolation chamber, a three-way ball valve B, a three-way ball valve C and a water receiving container; one end of the pipeline is connected with a high-pressure gas bottle, the other end of the pipeline is connected with one branch channel of the three-way ball valve B through a pressure reducing valve A, a gas buffer tank, a pressure reducing valve B, a pressure sensor and a switch, the other branch channel of the three-way ball valve B is connected with one branch channel of the three-way ball valve A through an organic solution isolation chamber, and a main channel of the three-way ball valve B is connected with one branch channel of the three-way ball valve C; the other channel of the three-way ball valve C is a water outlet and is communicated with a water receiving container, and the main channel of the three-way ball valve C is connected with the through hole B; the other branch channel of the three-way ball valve A is communicated with the through hole A, and the main channel of the three-way ball valve A is connected with the liquid pressure volume controller.
Preferably, the material of the test box door is a transparent organic glass plate, and a square grid is engraved on the transparent organic glass plate at a position corresponding to the inner cavity of the test box main body, namely, a grid measuring screen is formed at a position corresponding to the inner cavity of the test box main body on the test box door.
Preferably, a sealing gasket is arranged at the contact part of the test box door and the test box main body.
Preferably, the pore diameter of the permeable stone (19) is 50-500 μm.
Preferably, the coarse sand layer is composed of gravel with the grain diameter of 1 mm-3 mm.
The invention also provides a test method of the test device for repairing the air pressure splitting crack by the MICP technology according to any one of the claims 1-5, firstly, dividing the sample to be repaired into m types according to dry density, N types according to injection air pressure and x types according to bacteria liquid concentration, namely obtaining N types of preset samples with different dry densities, different injection air pressures and different bacteria liquid concentrations, wherein N is mxnxx; then carrying out a water injection saturation test, an air pressure splitting test, a microorganism grouting test and a repairing effect test on the N samples one by one, synchronously monitoring and recording the development condition of the cracks in the soil body and the microorganism repairing effect, and quantitatively evaluating the repairing effect;
any one of the predetermined samples in N is taken as a sample i, i is 1, 2, 3.. N, wherein the dry density of the sample i is ρdThe injection pressure is PjThe concentration of the bacterial liquid is Cc, d is 1, 2.. m, and j is 1, 2.. n; x is 1, 2.. x;
the procedure for testing sample i was as follows:
step 1, preparing a predetermined sample
Calculating the mass m of soil body required by filling the test box main bodysUniformly filling a soil body into the test box main body by a shakeout method to form a sample layer with the thickness of h 1; weighing a certain mass of coarse sand, and then uniformly filling the coarse sand on the upper part of the sample layer by a shakeout method to form a coarse sand layer with the thickness of h 2;
step 2, water flooding saturation test
Setting the water injection pressure as PZ(ii) a Will testThe box door is closed, the three-way ball valve A switch is opened, the liquid pressure volume controller is communicated with the through hole A, and the water injection pressure P is controlledZInjecting water; opening the three-way ball valve C to communicate the water outlet with the water receiving container; when the water injection amount of the liquid pressure volume controller in unit time is consistent with the water outlet amount of the sample i through the water outlet of one channel of the three-way ball valve C, the sample saturation is finished; closing the three-way ball valve A and the three-way ball valve C, and simultaneously calculating the saturated permeability coefficient K of the sample layer;
Figure BDA0002889092930000041
wherein the content of the first and second substances,
q is the water injection amount of the liquid pressure volume controller in unit time;
a is the cross-sectional area of the sample layer;
i is hydraulic gradient when water is injected for saturation, and I is 1/10PZ；
Step 3, air pressure splitting test
Step 3.1, opening a valve of the high-pressure gas cylinder, adjusting the pressure reducing valve A to enable gas to enter a gas buffer tank through a pipeline, and adjusting the gas pressure to injection gas pressure P through the pressure reducing valve B and the pressure sensorj；
Step 3.2, keeping the through hole A communicated with the outside; opening the switch, the three-way ball valve B and the three-way ball valve C to enable air pressure to enter the test box main body through the through hole B, and starting to perform an air pressure splitting test; monitoring the development and development states of soil body shallow cracks through a grid measuring screen at intervals of T1 in the test process, and measuring the volume of water discharged from the through hole A;
step 3.3, after the air pressure splitting test is carried out for T2 hours, checking whether a set termination condition is met, if the termination condition is met, ending the test, closing the three-way ball valve B and the three-way ball valve C, and entering the step 4; if the termination condition is not met, returning to the step 3.1 to continue the test;
the test termination conditions are as follows: the crack does not develop any more, and the volume of the discharged water is stable and unchanged;
step 4, microorganism grouting test
Step 4.1, preparing culture solution and microbial solution
The preparation process of the culture solution is as follows: weighing 20g of yeast extract, 10g of ammonium sulfate and 15.7482g of Tris by using an electronic balance, pouring the yeast extract, the ammonium sulfate and the Tris into a clean dry beaker together, then adding 1L of deionized water, fully stirring by using a glass rod, putting the beaker into an autoclave for sterilization treatment, after the sterilization is finished, putting the beaker under an ultraviolet lamp for irradiation for one hour, and cooling the beaker to room temperature to finish the preparation of a culture solution;
the preparation process of the microbial liquid comprises the following steps: pouring 200-250mL of culture solution into the conical flask after being sterilized under high pressure and cooled to room temperature on an aseptic test operating platform, extracting microbial strains by using a liquid transfer gun, injecting the microbial strains into the conical flask, mixing the microbial strains evenly, sealing the conical flask by using gauze, and then putting the conical flask into a constant-temperature oscillator for two-day bacterial liquid culture; after two days, adding a culture solution into the original bacterium solution for dilution to obtain a microbial bacterium solution with the set bacterium solution concentration Cc, and completing preparation of the microbial bacterium solution;
step 4.2, preparing the cementing liquid
22.2g of calcium chloride and 12g of urea are weighed by an electronic balance, poured into a clean dry beaker together, added with 200mL of deionized water and fully stirred by a glass rod, and the preparation of the cementing liquid is finished;
step 4.3, opening switches of the three-way ball valve B, the three-way ball valve A and the three-way ball valve C, enabling the liquid pressure volume controller to be communicated with the through hole B through the three-way ball valve A, the organic solution isolation chamber, the three-way ball valve B and the three-way ball valve C, and starting to perform a microorganism grouting test;
step 4.4, setting the pressure of the injected bacteria as Py; injecting the bacterial liquid into the organic solution isolation chamber, controlling the injection pressure Py through the liquid pressure volume controller to inject the bacterial liquid into the sample layer (17) from the through hole B, injecting the cementing liquid into the organic solution isolation chamber after 18 hours, controlling the injection pressure Py through the liquid pressure volume controller to inject the cementing liquid into the sample layer from the through hole B, and maintaining for 48 hours;
step 5, repair effect evaluation test
Step 5.1, opening the valve of the high-pressure gas cylinder, and adjusting the pressure reducing valve A to enable the gas to flowEnters a gas buffer tank through a pipeline, and the pressure is adjusted to the injection pressure P through a pressure reducing valve B and a pressure sensorj；
Step 5.2, keeping the through hole A communicated with the outside; opening the switch, the three-way ball valve B and the three-way ball valve C to enable air pressure to enter the test box main body, starting an air pressure splitting test, monitoring the development and development states of soil body shallow cracks through the grid measuring screen at intervals of T1 in the test process, and measuring the volume of water discharged from the through hole A;
step 5.3, after the air pressure splitting test is carried out for T2 hours, checking whether a set termination condition is met, if the termination condition is met, ending the test, closing the three-way ball valve B and the three-way ball valve C, and entering step 5.4; if the termination condition is not met, defining the repair failure, recording the state of the crack, and finishing the test;
the test termination conditions are as follows: the crack does not develop any more, and the volume of the discharged water is stable and unchanged;
step 5.4, the water injection saturation test in the step 2 is carried out on the sample layer subjected to the air pressure splitting again, and the saturation permeability coefficient K of the sample layer subjected to the splitting is obtained1And calculating the repairing effect constant R,
Figure BDA0002889092930000061
and 6, checking whether a predetermined sample is still left in the m multiplied by n multiplied by x predetermined samples and not tested, if so, returning to the step 1 to test the next predetermined sample, and if not, finishing the test.
Preferably, said water injection pressure PZ5 kPa-30 kPa, and the pressure Py for injecting the bacteria is 30 kPa-50 kPa.
Preferably, the strain in the microbial liquid is bacillus pasteurii.
Preferably, the deionized water has a pH of 9.02.
Compared with the prior art, the invention has the beneficial effects that:
1. by measuring the volume of the water discharged from the sample during the air pressure splitting process, the volume can be used as an index for judging the development of cracks in the air pressure splitting test of the sample.
2. The test box door can engrave square grid to form grid measuring screen, which is convenient for directly observing the development of soil body shallow layer crack in the air pressure splitting process.
3. The test device can control different dry densities, different injection pressures and microbial liquid concentrations, and observe the development condition of the sample layer crack and the microbial remediation effect.
Drawings
Fig. 1 is a schematic view of the overall structure in the embodiment of the present invention.
Figure 2 is a side view of a test chamber of the present invention.
Fig. 3 is a right side view of fig. 2.
FIG. 4 is a flow chart of an assay method of the present invention.
In the figure: 1. a high pressure gas cylinder; 2. a pipeline; 3. a pressure reducing valve A; 4. a gas buffer tank; 5. a pressure reducing valve B; 6. a pressure sensor; 7. a switch; 8. a liquid pressure volume controller; 9. a three-way ball valve A; 10. an organic solution isolation chamber; 11. a three-way ball valve B; 12. a three-way ball valve C; 13. a water receiving container; 14. a test box main body; 15. a coarse sand layer; 16. a through hole A; 17. a sample layer; 18. a through hole B; 19. a permeable stone; 20. a test box door; 21. a grid measuring screen; 22. and a gasket.
Detailed Description
Embodiments of the present invention are described in detail below with reference to fig. 1-3.
As can be seen from fig. 1 to 3, the test device for repairing a crack by air pressure cleavage using the MICP technique according to the embodiment of the present invention includes a test chamber main body 14, a test chamber door 20, and an auxiliary device. The cross section of the test box main body 14 is rectangular, a permeable stone 19 is embedded in the center of the bottom of the test box main body 14, a through hole B18 is reserved, the through hole B18 is communicated with the permeable stone 19, and through holes A16 are uniformly distributed on the top of the test box main body 14. The test box door 20 is closed during the test, so as to seal the inner cavity of the test box main body 14.
In this embodiment, the diameter of the porous stone 19 is 50 μm to 500. mu.m. The contact part of the test box door 20 and the test box main body 14 is provided with a sealing gasket 22. The test box door 20 is made of transparent organic glass plate, square grids are engraved on the transparent organic glass plate at the position corresponding to the inner cavity of the test box main body 14, namely, a grid measuring screen 21 is formed at the position, corresponding to the inner cavity of the test box main body 14, of the test box door 20. The specific structure of the grid measuring screen 21 is shown in fig. 3.
In the test box main body 14, a sample layer 17 composed of a sample and a coarse sand layer 15 composed of coarse sand are respectively arranged from bottom to top, wherein the layer thickness of the sample layer 17 is H1, the layer thickness of the coarse sand layer 15 is H2, H is equal to or less than H which is H1+ H2, and H is the height of the inner cavity of the test box main body 14. In this embodiment, the coarse sand layer (15) is composed of gravel having a grain size of 1mm to 3 mm.
The auxiliary device comprises a high-pressure gas bottle 1, a pipeline 2, a pressure reducing valve A3, a gas buffer tank 4, a pressure reducing valve B5, a pressure sensor 6, a switch 7, a liquid pressure volume controller 8, a three-way ball valve A9, an organic solution isolation chamber 10, a three-way ball valve B11, a three-way ball valve C12 and a water receiving container 13. One end of the pipeline 2 is connected with a high-pressure gas bottle 1, the other end of the pipeline is connected with one channel of a three-way ball valve B11 through a pressure reducing valve A3, a gas buffer tank 4, a pressure reducing valve B5, a pressure sensor 6 and a switch 7, the other channel of the three-way ball valve B11 is connected with one channel of the three-way ball valve A9 through an organic solution isolation chamber 10, and a main channel of the three-way ball valve B11 is connected with one channel of the three-way ball valve C12. The other branch passage of the three-way ball valve C12 is a water outlet and is communicated with a water receiving container 13, and the main passage of the three-way ball valve C12 is connected with the through hole B18. The other branch channel of the three-way ball valve A9 is communicated with a through hole A16, and the main channel of the three-way ball valve A9 is connected with the liquid pressure volume controller 8.
Specifically, in this example, the thickness of the sample layer 17 was 350mm, and the thickness of the coarse sand layer 15 was 50 mm. The test box main body 14 and the test box door 20 are made of cast plexiglass, wherein the test box main body 14 is 540mm long, 440mm wide and 70mm thick. The porous stone 19 is stainless steel porous stone with diameter of 25 + -0.5 mm, thickness of 0.3mm, pore size of 55 μm, and error of less than 5.0 μm. The pressure measuring range of the pressure sensor 6 is 0.001-10 MPa. The internal dimensions of the test box body 14 are: 500mm long, 400mm wide and 30mm thick. The sample test chamber door 20 is made of organic glass plate, and the size is: 540mm long, 440mm wide and 20mm thick. The size of the grid measuring screen 21 is as follows: 500mm long and 400mm wide, and is divided into 2000 squares of the same size by the grid.
In this embodiment, the high pressure gas cylinder 1 can provide an injection pressure of 0 to 10 MPa. The ranges of the pressure reducing valve A3 and the pressure reducing valve B5 are both 0.1-10 MPa. The pressure of the gas buffer tank 4 is 10 MPa. The range of the pressure sensor 6 is 10MPa, and the accuracy is 0.1 kPa.
The pressure reducing valve A3, the gas buffer tank 4, the pressure reducing valve B5, the pressure sensor 6, the switch 7, the three-way ball valve A9, the three-way ball valve B11 and the three-way ball valve C12 are all made of 316L low-carbon stainless steel.
The invention also provides a test method for repairing the air pressure splitting crack by the M-working CP technology, which comprises the steps of firstly dividing the samples to be repaired into M types according to dry density, N types according to injection air pressure and x types according to bacteria liquid concentration to obtain N types of preset samples with different dry densities, different injection air pressures and different bacteria liquid concentrations, wherein N is mxnxx; then carrying out a water injection saturation test, an air pressure splitting test, a microorganism grouting test and a repairing effect test on the N samples one by one, synchronously monitoring and recording the development condition of the cracks in the soil body and the microorganism repairing effect, and quantitatively evaluating the repairing effect;
any one of the predetermined samples in N is taken as a sample i, i is 1, 2, 3.. N, wherein the dry density of the sample i is ρdThe injection pressure is PjThe concentration of the bacterial liquid is Cc, d is 1, 2.. m, and j is 1, 2.. n; x is 1, 2.. x;
FIG. 4 is a flow chart of an assay method of the present invention. As can be seen from FIG. 4, the procedure for testing sample i is as follows:
step 1, preparing a predetermined sample
Calculating the mass m of soil mass required by the filling test box main body 14sUniformly filling a soil body into the test box main body 14 by a shakeout method to form a sample layer 17 with the layer thickness of h 1; weighing a certain mass of coarse sand, and then uniformly filling the coarse sand on the upper part of the sample layer 17 by a shakeout method to form a coarse sand layer 15 with the thickness of h2。
Step 2, water flooding saturation test
Setting the water injection pressure as PZ(ii) a The test box door 20 is closed, the three-way ball valve A9 switch is opened, the liquid pressure volume controller 8 is communicated with the through hole A16, and the water injection pressure P is controlledZInjecting water; opening the three-way ball valve C12 to communicate the water outlet with the water container; when the water injection amount of the liquid pressure volume controller 8 in unit time is consistent with the water outlet amount of the sample i through the water outlet of one channel of the three-way ball valve C12, the sample saturation is finished; closing the three-way ball valve A9 and the three-way ball valve C12, and simultaneously calculating the saturation permeability coefficient K of the sample layer 17;
Figure BDA0002889092930000101
wherein the content of the first and second substances,
q is the water injection amount of the liquid pressure volume controller in unit time;
a is the cross-sectional area of the sample layer;
i is hydraulic gradient when water is injected for saturation, and I is 1/10PZ。
In the present embodiment, the water injection pressure PZ5kPa to 30 kPa.
Step 3, air pressure splitting test
Step 3.1, opening a valve of the high-pressure gas bottle 1, adjusting a pressure reducing valve A3 to enable gas to enter a gas buffer tank 4 through a pipeline 2, and adjusting the gas pressure to injection gas pressure P through a pressure reducing valve B5 and a pressure sensor 6j；
Step 3.2, keeping the through hole A16 communicated with the outside; opening the switch 7, the three-way ball valve B11 and the three-way ball valve C12, enabling air pressure to enter the test box body 14 through the through hole B18, and starting to perform an air pressure splitting test; in the test process, the development and development states of soil body shallow cracks are monitored through the grid measuring screen 21 every T1 time, and the volume of water discharged from the through holes A16 is measured;
step 3.3, after the air pressure splitting test is carried out for T2 hours, checking whether a set termination condition is met, if the termination condition is met, ending the test, closing the three-way ball valve B11 and the three-way ball valve C12, and entering the step 4; if the termination condition is not met, returning to the step 3.1 to continue the test;
the test termination conditions are as follows: the crack does not develop any more, and the volume of the discharged water is stable and unchanged;
step 4, microorganism grouting test
Step 4.1, preparing culture solution and microbial solution
The preparation process of the culture solution is as follows: weighing 20g of yeast extract, 10g of ammonium sulfate and 15.7482g of Tris by using an electronic balance, pouring the yeast extract, the ammonium sulfate and the Tris into a clean dry beaker together, then adding 1L of deionized water, fully stirring by using a glass rod, putting the beaker into an autoclave for sterilization treatment, after the sterilization is finished, putting the beaker under an ultraviolet lamp for irradiation for one hour, and cooling the beaker to room temperature to finish the preparation of a culture solution;
the preparation process of the microbial liquid comprises the following steps: pouring 200-250mL of culture solution into the conical flask after being sterilized under high pressure and cooled to room temperature on an aseptic test operating platform, extracting microbial strains by using a liquid transfer gun, injecting the microbial strains into the conical flask, mixing the microbial strains evenly, sealing the conical flask by using gauze, and then putting the conical flask into a constant-temperature oscillator for two-day bacterial liquid culture; after two days, adding a culture solution into the original bacterium solution for dilution to obtain a microbial bacterium solution with the set bacterium solution concentration Cc, and completing preparation of the microbial bacterium solution;
step 4.2, preparing the cementing liquid
22.2g of calcium chloride and 12g of urea are weighed by an electronic balance, poured into a clean dry beaker together, added with 200mL of deionized water and fully stirred by a glass rod, and the preparation of the cementing liquid is finished;
step 4.3, opening switches of a three-way ball valve B11, a three-way ball valve A9 and a three-way ball valve C12 to enable the liquid pressure volume controller 8 to be communicated with a through hole B18 through the three-way ball valve A9, the organic solution isolation chamber 10, the three-way ball valve B11 and the three-way ball valve C12, and starting a microorganism grouting test;
step 4.4, setting the pressure of the injected bacteria as Py; injecting the bacterial liquid into the organic solution isolation chamber 10, controlling the bacterial injection pressure Py through the liquid pressure volume controller 8 to inject the bacterial liquid into the sample layer 17 from the through hole B18, injecting the cementing liquid into the organic solution isolation chamber 10 after 18 hours, controlling the bacterial injection pressure Py through the liquid pressure volume controller 8 to inject the cementing liquid into the sample layer 17 from the through hole B18, and maintaining for 48 hours.
In this embodiment, the strain in the microbial liquid is bacillus pasteurii, the pH of the deionized water is 9.02, and the injection pressure Py is 30kPa to 50 kPa.
Step 5, repair effect evaluation test
Step 5.1, opening a valve of the high-pressure gas bottle 1, adjusting a pressure reducing valve A3 to enable gas to enter a gas buffer tank 4 through a pipeline 2, and adjusting the gas pressure to injection gas pressure P through a pressure reducing valve B5 and a pressure sensor 6j；
Step 5.2, keeping the through hole A16 communicated with the outside; opening the switch 7, the three-way ball valve B11 and the three-way ball valve C12 to enable air pressure to enter the test box main body 14, starting an air pressure splitting test, monitoring the development and development state of soil body shallow cracks through the grid measuring screen 21 every T1 time in the test process, and measuring the volume of water discharged from the through hole A16;
step 5.3, after the air pressure splitting test is carried out for T2 hours, checking whether a set termination condition is met, if the termination condition is met, ending the test, closing the three-way ball valve B11 and the three-way ball valve C12, and entering step 5.4; if the termination condition is not met, defining the repair failure, recording the state of the crack, and finishing the test;
the test termination conditions are as follows: the crack does not develop any more, and the volume of the discharged water is stable and unchanged;
step 5.4, the water injection saturation test in the step 2 is carried out on the sample layer subjected to the air pressure splitting again, and the saturation permeability coefficient K of the sample layer subjected to the splitting is obtained1And calculating a repairing effect constant R.
Figure BDA0002889092930000121
And 6, checking whether a predetermined sample is still left in the m multiplied by n multiplied by x predetermined samples and not tested, if so, returning to the step 1 to test the next predetermined sample, and if not, finishing the test.
In this example, T1 was 2 hours and T2 was 24 hours.
In this embodiment, m is 3, N is 9, x is 5, and N is 135, that is, 135 predetermined samples are obtained. Wherein, the 3 dry densities are: 1.6g/cm3、1.7g/cm3、1.8g/cm3. The 9 injection pressures are: 20kPa, 40kPa, 60kPa, 80kPa, 100kPa, 120kPa, 140kPa, 160kPa, 180 kPa; the concentration of 5 bacterial solutions is: 1.2mol/L, 1.4mol/L, 1.6mol/L, 1.8mol/L and 2.0 mol/L.
In this embodiment, the repairing effect is evaluated according to the repairing effect constant R as follows:
when R is more than or equal to 0 and less than or equal to 20 percent, the repairing effect is good;
when R is more than 20 and less than or equal to 40 percent, the repairing effect is better;
when R is more than 40 and less than or equal to 60 percent, the repairing effect is poor;
when R is more than 60 and less than or equal to 80 percent, the repairing effect is poor;
when R is more than 80 and less than or equal to 100 percent, the repairing effect is extremely poor.
The invention respectively carries out air pressure splitting, microorganism grouting test and repairing effect evaluation test on 135 kinds of preset samples one by one, records the test results, provides enough samples for repairing air pressure splitting cracks by the MICP technology, and can complete the formulation and optimization of repairing schemes by means of the samples.

Claims (9)

1. A test device for repairing an air pressure fracture by an MICP technology is characterized by comprising a test box main body (14), a test box door (20) and an auxiliary device; the cross section of the test box main body (14) is rectangular, a permeable stone (19) is embedded in the center of the bottom of the test box main body (14), a through hole B (18) is reserved, the through hole B (18) is communicated with the permeable stone (19), and through holes A (16) are uniformly distributed in the top of the test box main body (14); the test box door (20) is closed during testing, so that the inner cavity of the test box main body (14) is sealed;
in the test box main body (14), a sample layer (17) formed by a sample and a coarse sand layer (15) formed by coarse sand are respectively arranged from bottom to top, wherein the layer thickness of the sample layer (17) is H1, the layer thickness of the coarse sand layer (15) is H2, H1+ H2 is more than or equal to H, and H is the height of an inner cavity of the test box main body (14);
the auxiliary device comprises a high-pressure gas bottle (1), a pipeline (2), a pressure reducing valve A (3), a gas buffer tank (4), a pressure reducing valve B (5), a pressure sensor (6), a switch (7), a liquid pressure volume controller (8), a three-way ball valve A (9), an organic solution isolation chamber (10), a three-way ball valve B (11), a three-way ball valve C (12) and a water receiving container (13); one end of the pipeline (2) is connected with the high-pressure gas bottle (1), the other end of the pipeline is connected with one channel of the three-way ball valve B (11) through the pressure reducing valve A (3), the gas buffer tank (4), the pressure reducing valve B (5), the pressure sensor (6) and the switch (7), the other channel of the three-way ball valve B (11) is connected with one channel of the three-way ball valve A (9) through the organic solution isolation chamber (10), and the main channel of the three-way ball valve B (11) is connected with one channel of the three-way ball valve C (12); the other branch channel of the three-way ball valve C (12) is a water outlet and is communicated with a water receiving container (13), and the main channel of the three-way ball valve C (12) is connected with the through hole B (18); the other branch channel of the three-way ball valve A (9) is communicated with the through hole A (16), and the main channel of the three-way ball valve A (9) is connected with the liquid pressure volume controller (8).
2. The test device for repairing the air pressure crack by the MICP technology as claimed in claim 1, wherein the test chamber door (20) is made of transparent organic glass plate, square grids are engraved on the transparent organic glass plate at the position corresponding to the inner cavity of the test chamber body (14), namely, a grid measuring screen (21) is formed on the test chamber door (20) at the position corresponding to the inner cavity of the test chamber body (14).
3. The MICP technical repairing test device for air pressure splitting crack according to claim 1, wherein the contact part of the test box door (20) and the test box main body (14) is provided with a sealing gasket (22).
4. The MICP technical testing device for repairing the air pressure splitting crack according to claim 1, wherein the pore diameter of the permeable stone (19) is 50 μm to 500 μm.
5. The MICP technical repair pneumatic fracture test device according to claim 1, wherein the coarse sand layer (15) is composed of gravel with a grain size of 1 mm-3 mm.
6. A test method of a test device for repairing air pressure splitting cracks by MICP technology according to any one of claims 1-5, characterized in that, firstly, samples to be repaired are divided into m types according to dry density, N types according to injection air pressure and x types according to bacteria liquid concentration, thus obtaining N types of preset samples with different dry density, different injection air pressure and different bacteria liquid concentration, wherein N is m x N x; then carrying out a water injection saturation test, an air pressure splitting test, a microorganism grouting test and a repairing effect test on the N samples one by one, synchronously monitoring and recording the development condition of the cracks in the soil body and the microorganism repairing effect, and quantitatively evaluating the repairing effect;
any one of the predetermined samples in N is designated as a sample i, i is 1, 2, 3 … N, where the dry density of the sample i is ρdThe injection pressure is PjThe concentration of the bacterial liquid is Cc, d is 1, 2 … m, j is 1, 2 … n; c is 1, 2 … x;
the procedure for testing sample i was as follows:
step 1, preparing a predetermined sample
Calculating the mass m of the soil body required by the filling test box main body (14)sUniformly filling a soil body into the test box main body (14) by a shakeout method to form a sample layer (17) with the layer thickness of h 1; weighing a certain mass of coarse sand, and then uniformly filling the coarse sand on the upper part of a sample layer (17) by a shakeout method to form a coarse sand layer (15) with the layer thickness of h2；
Step 2, water flooding saturation test
Setting the water injection pressure as PZ(ii) a The test box door (20) is closed, the three-way ball valve A (9) is opened to communicate the liquid pressure volume controller (8) with the through hole A (16) and control the water injection pressure PZInjecting water; opening the three-way ball valve C (12) to communicate the water outlet with the water container; as the liquid pressure volume is controlled per unit timeWhen the water injection amount of the preparation device (8) is consistent with the water outlet amount of the sample i through a water outlet of one channel of the three-way ball valve C (12), the sample saturation is finished; closing the three-way ball valve A (9) and the three-way ball valve C (12), and simultaneously calculating the saturated permeability coefficient K of the sample layer (17);
Figure FDA0002889092920000031
wherein the content of the first and second substances,
q is the water injection amount of the liquid pressure volume controller in unit time;
a is the cross-sectional area of the sample layer;
i is the hydraulic gradient when the water is saturated,
Figure FDA0002889092920000032
step 3, air pressure splitting test
Step 3.1, opening a valve of the high-pressure gas bottle (1), adjusting a pressure reducing valve A (3) to enable gas to enter a gas buffer tank (4) through a pipeline (2), and adjusting the gas pressure to injection gas pressure P through a pressure reducing valve B (5) and a pressure sensor (6)j；
Step 3.2, keeping the through hole A (16) communicated with the outside; opening a switch (7), a three-way ball valve B (11) and a three-way ball valve C (12) to enable air pressure to enter a test box body (14) through a through hole B (18), and starting an air pressure splitting test; in the test process, the development and development states of soil body shallow cracks are monitored through the grid measuring screen (21) every T1 time, and the volume of water discharged from the through hole A (16) is measured;
step 3.3, after the air pressure splitting test is carried out for T2 hours, checking whether a set termination condition is met, if the termination condition is met, ending the test, closing the three-way ball valve B (11) and the three-way ball valve C (12), and entering step 4; if the termination condition is not met, returning to the step 3.1 to continue the test;
the test termination conditions are as follows: the crack does not develop any more, and the volume of the discharged water is stable and unchanged;
step 4, microorganism grouting test
Step 4.1, preparing culture solution and microbial solution
The preparation process of the culture solution is as follows: weighing 20g of yeast extract, 10g of ammonium sulfate and 15.7482g of Tris by using an electronic balance, pouring the yeast extract, the ammonium sulfate and the Tris into a clean dry beaker together, then adding 1L of deionized water, fully stirring by using a glass rod, putting the beaker into an autoclave for sterilization treatment, after the sterilization is finished, putting the beaker under an ultraviolet lamp for irradiation for one hour, and cooling the beaker to room temperature to finish the preparation of a culture solution;
the preparation process of the microbial liquid comprises the following steps: pouring 200-250mL of culture solution into the conical flask after being sterilized under high pressure and cooled to room temperature on an aseptic test operating platform, extracting microbial strains by using a liquid transfer gun, injecting the microbial strains into the conical flask, mixing the microbial strains evenly, sealing the conical flask by using gauze, and then putting the conical flask into a constant-temperature oscillator for two-day bacterial liquid culture; after two days, adding a culture solution into the original bacterium solution for dilution to obtain a microbial bacterium solution with the set bacterium solution concentration Cc, and completing preparation of the microbial bacterium solution;
step 4.2, preparing the cementing liquid
22.2g of calcium chloride and 12g of urea are weighed by an electronic balance, poured into a clean dry beaker together, added with 200mL of deionized water and fully stirred by a glass rod, and the preparation of the cementing liquid is finished;
step 4.3, opening switches of a three-way ball valve B (11), a three-way ball valve A (9) and a three-way ball valve C (12) to enable a liquid pressure volume controller (8) to be communicated with a through hole B (18) through the three-way ball valve A (9), an organic solution isolation chamber (10), the three-way ball valve B (11) and the three-way ball valve C (12), and starting a microorganism grouting test;
step 4.4, setting the pressure of the injected bacteria as Py; injecting the bacterial liquid into the organic solution isolation chamber (10), controlling the bacterial injection pressure Py through the liquid pressure volume controller (8) to inject the bacterial liquid into the sample layer (17) from the through hole B (18), injecting the cementing liquid into the organic solution isolation chamber (10) after 18 hours, controlling the bacterial injection pressure Py through the liquid pressure volume controller (8) to inject the cementing liquid into the sample layer (17) from the through hole B (18), and maintaining for 48 hours;
step 5, repair effect evaluation test
Step 5.1, opening a valve of the high-pressure gas bottle (1), and adjusting a pressure reducing valve A (3) to enable gas to pass throughThe pipeline (2) enters a gas buffer tank (4), and the pressure is adjusted to injection pressure P by a pressure reducing valve B (5) and a pressure sensor (6)j；
Step 5.2, keeping the through hole A (16) communicated with the outside; opening a switch (7), a three-way ball valve B (11) and a three-way ball valve C (12) to enable air pressure to enter a test box main body (14) to start an air pressure splitting test, monitoring the development and development state of soil body shallow cracks through a grid measuring screen (21) every T1 time in the test process, and measuring the volume of water discharged from a through hole A (16);
step 5.3, after the air pressure splitting test is carried out for T2 hours, checking whether a set termination condition is met, if the termination condition is met, ending the test, closing the three-way ball valve B (11) and the three-way ball valve C (12) and entering the step 5.4; if the termination condition is not met, defining the repair failure, recording the state of the crack, and finishing the test;
the test termination conditions are as follows: the crack does not develop any more, and the volume of the discharged water is stable and unchanged;
step 5.4, the water injection saturation test in the step 2 is carried out on the sample layer subjected to the air pressure splitting again, and the saturation permeability coefficient K of the sample layer subjected to the splitting is obtained1And calculating the repairing effect constant R,
Figure FDA0002889092920000051
and 6, checking whether a predetermined sample is still left in the m multiplied by n multiplied by x predetermined samples and not tested, if so, returning to the step 1 to test the next predetermined sample, and if not, finishing the test.
7. The testing method of the testing device for repairing the air pressure fracture by the MICP technology according to claim 6, wherein the water injection pressure PZ5 kPa-30 kPa, and the pressure Py for injecting the bacteria is 30 kPa-50 kPa.
8. The test method of the test device for repairing the air pressure fracture by the MICP technology according to claim 6, wherein the strain in the microbial fluid is Bacillus pasteurianus.
9. The testing method of the testing apparatus for repairing a pneumatic fracture by MICP technology according to claim 6, wherein the pH value of the deionized water is 9.02.
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