CN110082506A - A kind of test reaction unit and test reaction method - Google Patents
A kind of test reaction unit and test reaction method Download PDFInfo
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- CN110082506A CN110082506A CN201910418502.9A CN201910418502A CN110082506A CN 110082506 A CN110082506 A CN 110082506A CN 201910418502 A CN201910418502 A CN 201910418502A CN 110082506 A CN110082506 A CN 110082506A
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Abstract
The invention discloses a kind of test reaction units, regulate and control lower microorganism grouting and reinforcing sand body for organic substrate, the support device for being placed with sand body is provided in reaction vessel, the top of reaction vessel offers solution grouting port and bacterium solution grouting port, channel is provided in reaction vessel, the top in channel is connected to solution grouting port and bacterium solution grouting port, its bottom is located at the top of sand body, the sample tap and exhaust outlet being connected to channel are offered on reaction vessel, slip casting uniform device is provided in reaction vessel, hold-up vessel is connected to by connecting pipe with solution grouting port, first pump housing is provided on connecting pipe, the bottom end of reaction vessel is provided with leakage fluid dram, the first water check valve is provided on leakage fluid dram, the both ends of by-pass line are connected to the both ends of reaction vessel respectively, the bottom end connectivity part of by-pass line and reaction vessel setting the Two water check valves.It can be improved the waterproofness of sampling non-destructive, sample uniformity and device.The invention also discloses a kind of test reaction methods.
Description
Technical field
The present invention relates to sand body reinforcement experiment technical field, in particular to a kind of test reaction unit and test reaction side
Method.
Background technique
Current microorganism grouting reinforcement technique (MICP) obtains carbonate using Bacillus pasteurii hydrolysis urea mostly
Ion, by generating precipitation of calcium carbonate with the calcium binding of external source, thus cementing sand grains, and then reach improvement sand body
The purpose of physical and mechanical property.But it is found in the long-term experiment process of argumentation, effect is reinforced in precipitation of calcium carbonate acquired in the technology
Fruit is also unsatisfactory, is mainly manifested in that calcium carbonate crystal conversion ratio is high, binder soil granule strength is insufficient, corrosion resistance and water-fast
Several aspects such as property is poor.For this purpose, organic substrate appropriate is added, by the crystal form, brilliant looks and the crystallite dimension that regulate and control crystalline solid
Etc. features, can improve conscientiously there is currently many drawbacks and microorganism reinforce aspect advanced problems.
Researchers at home and abroad experimental provision employed in microorganism grouting and reinforcing sand body mostly has following total
Same point: 1. its microorganism grouting and reinforcing sand body device mainly use immersion way to reinforce；2. reaction vessel is generally organic glass
Glass column；3. reaction unit is connected and fixed using form of flanges.Though above-mentioned experimental method achieves certain breakthrough to a certain extent,
But still there are many deficiencies.For example the high temperature limit of Organic glass column is mostly at 80 DEG C or so, thus reaction unit is in reality
It can not thoroughly be sterilized before testing, be easy to cause inside container and generate complicated bacterium colony, interference experiment result；Flange connections simultaneously
Usually since bolt does not fasten and generation device leak gas leak phenomenon, and bolt tension damages flange bolt mouth cracking even
It is bad；Secondly, reaction under soaking conditions can not fluid flow state under real simulation natural conditions, easily cause sand in plane
Uneven reinforcing on upper and vertical；In addition, device inside microorganism is generated due to oxygen consumption after experiment reaction a period of time
The gases such as carbon dioxide excessively will cause air pressure inside raising, while the oxygen wretched insufficiency for causing Institute of Micro-biology to need, Jin Erying
Ring consolidation effect；Finally, current sand bracing means mostly by the wire netting timing sampling of pre-buried cylinder sample size or
By the way that a large amount of experimental comparison group is arranged, device sampling is removed after reaction wait test, iron hoop sampling mode is easily broken at this time
The original structure of bad sand body, and the setting of multiple control groups sampling can make extend at double experimental period.
Therefore, a kind of test reaction unit how is provided, to improve sampling non-destructive, sample uniformity and device itself
Waterproofness is a technical problem that technical personnel in the field need to solve at present.
Summary of the invention
The object of the present invention is to provide a kind of test reaction units, to improve sampling non-destructive, sample uniformity and device
The waterproofness of itself；It is a further object of the present invention to provide a kind of test reaction methods based on above-mentioned test reaction unit.
In order to solve the above technical problems, the present invention provides following scheme:
A kind of test reaction unit, microorganism grouting and reinforcing sand body under regulating and controlling for organic substrate, including hold-up vessel,
First pump housing, second pump housing, connecting pipe, reaction vessel and by-pass line, wherein
Support device is provided in the reaction vessel, the support device offers leak hole, puts in the support device
It is equipped with sand body,
The top of the reaction vessel offers solution grouting port and bacterium solution grouting port, is provided in the reaction vessel logical
Road, the top in the channel are connected to the solution grouting port and the bacterium solution grouting port, and bottom is located at the sand body
Top, the channel include the first vertical section being sequentially communicated, tilting section and the second vertical section,
The sample tap and exhaust outlet being connected to the channel are offered on the reaction vessel,
It is provided with slip casting uniform device in the reaction vessel and between second vertical section and the sand body,
The hold-up vessel is connected to by the connecting pipe with the solution grouting port, is provided on the connecting pipe
First pump housing,
The bottom end of the reaction vessel is provided with leakage fluid dram, and the first water check valve is provided on the leakage fluid dram,
The top of the by-pass line is connected to the reaction vessel, and communicating position is located under the slip casting uniform device
Side, the bottom end of the by-pass line is connected to the bottom end of the reaction vessel and connectivity part is provided with the second water check valve.
Preferably, geotextiles are equipped on above-mentioned sand body, offer in the geotextiles and is reserved for what soil sampler passed through
Hole.
Preferably, above-mentioned support device is protection support lattice disk.
Preferably, above-mentioned first pump housing is peristaltic pump, and second pump housing is pressure pump.
Preferably, the tilt angle of above-mentioned tilting section with respect to the horizontal plane is 10 °.
Preferably, above-mentioned reaction vessel is made of upper cover and lower case, the upper cover and the lower case
Body is by the screw socket connection that is screwed on, and the junction of the two is between the slip casting uniform device and the sand body.
Preferably, the glass handle for rotating the sand body is provided on above-mentioned reaction vessel.
Preferably, damping device is provided with below above-mentioned reaction vessel.
The present invention also provides a kind of test reaction methods, regulate and control lower microorganism grouting and reinforcing sand body for organic substrate,
Based on test reaction unit described in above-mentioned any one, comprising:
It will be configured and undergo the reaction solution mixed injection institute including culture medium, calcium chloride and urea of disinfecting action
It states in hold-up vessel and saves,
First pump housing is opened when slip casting, after the reaction solution flows through connecting pipe, by solution grouting port, flows into institute
Channel is stated, meanwhile, during injecting the reaction solution, by the good fixed concentration of shaken cultivation in sterile working room
Bacillus pasteurii bacterium solution injects the channel, the Bacillus pasteurii bacterium solution reacts molten with described by bacterium solution grouting port
After liquid is sufficiently mixed at the connection turning of first vertical section and the tilting section, the ramp along the tilting section is flow to
The slip casting uniform device makes mixed reaction solution uniformly splash down the inside circumference in the reaction vessel；
When being reacted or being sampled using immersion type, the reaction solution in the reaction vessel is pumped by second pump housing
In the by-pass line, second water check valve is simultaneously closed off；
When using circulating reaction, then will second water check valve opening, by second pump housing by the reaction
Reaction solution in container is sucked in the by-pass line and is recycled, and flow to the sandy soils top again, make reaction solution according to
The flowing of clockwise direction iterative cycles, when the sand body vertically reinforces uneven, can make second pump housing reversed
It pumps, flows reaction solution according to counter clockwise direction；
When carrying out solution sampling, first water check valve is opened, when carrying out sand sampling, by soil sampler from institute
Sample tap is stated to protrude into.
Preferably, above-mentioned test reaction method further includes before starting test,
After cleaning the test reaction unit, the sand body is sprinkled into the reaction vessel, then the test
Each section of reaction unit is put into autoclave the 20min that persistently sterilizes under the conditions of 121 DEG C, then completes in sterile working room
Device splicing and assembling, and by the pre-configured reaction solution including culture medium, calcium chloride and urea, pour into the storage
It is stand-by in container.
Reaction unit is tested provided by aforementioned present invention, regulates and controls lower microorganism grouting and reinforcing sand for organic substrate
Body, including hold-up vessel, first pump housing, second pump housing, connecting pipe, reaction vessel and by-pass line, wherein
Support device is provided in the reaction vessel, the support device offers leak hole, puts in the support device
It is equipped with sand body,
The top of the reaction vessel offers solution grouting port and bacterium solution grouting port, is provided in the reaction vessel logical
Road, the top in the channel are connected to the solution grouting port and the bacterium solution grouting port, and bottom is located at the sand body
Top, the channel include the first vertical section being sequentially communicated, tilting section and the second vertical section,
The sample tap and exhaust outlet being connected to the channel are offered on the reaction vessel,
It is provided with slip casting uniform device in the reaction vessel and between second vertical section and the sand body,
The hold-up vessel is connected to by the connecting pipe with the solution grouting port, is provided on the connecting pipe
First pump housing,
The bottom end of the reaction vessel is provided with leakage fluid dram, and the first water check valve is provided on the leakage fluid dram,
The top of the by-pass line is connected to the reaction vessel, and communicating position is located under the slip casting uniform device
Side, the bottom end of the by-pass line is connected to the bottom end of the reaction vessel and connectivity part is provided with the second water check valve.
In use, will be configured and the reaction solution including culture medium, calcium chloride and urea of disinfecting action is undergone to mix
It closes to inject in the hold-up vessel and save,
First pump housing is opened when slip casting, after the reaction solution flows through connecting pipe, by solution grouting port, flows into institute
Channel is stated, meanwhile, during injecting the reaction solution, by the good fixed concentration of shaken cultivation in sterile working room
Bacillus pasteurii bacterium solution injects the channel, the Bacillus pasteurii bacterium solution reacts molten with described by bacterium solution grouting port
After liquid is sufficiently mixed at the connection turning of first vertical section and the tilting section, the ramp along the tilting section is flow to
The slip casting uniform device makes mixed reaction solution uniformly splash down the inside circumference in the reaction vessel；
When being reacted or being sampled using immersion type, the reaction solution in the reaction vessel is pumped by second pump housing
In the by-pass line, second water check valve is simultaneously closed off；
When using circulating reaction, then will second water check valve opening, by second pump housing by the reaction
Reaction solution in container is sucked in the by-pass line and is recycled, and flow to the sandy soils top again, make reaction solution according to
The flowing of clockwise direction iterative cycles, when the sand body vertically reinforces uneven, can make second pump housing reversed
It pumps, flows reaction solution according to counter clockwise direction；
When carrying out solution sampling, first water check valve is opened, when carrying out sand sampling, by soil sampler from institute
Sample tap is stated to protrude into.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of test reaction unit provided by the embodiment of the present invention；
Fig. 2 is the structural schematic diagram figure of geotextiles provided by the embodiment of the present invention；
Fig. 3 is the structural schematic diagram figure of protection support lattice disk provided by the embodiment of the present invention；
Fig. 4 is the structural schematic diagram of slip casting uniform device provided by the embodiment of the present invention.
In figure 1 above -4:
Hold-up vessel 1, first pump housing 2, connecting pipe 3, sand body 4, protection support lattice disk 5, by-pass line 6, geotextiles 7,
Slip casting uniform device 8, the first vertical section 9, tilting section 10, the second vertical section 11, channel 12, soil sampler 13, sample tap 14, exhaust
Mouth 15, second pump housing 16, glass handle 17, the first water check valve 18, the second water check valve 19, leakage fluid dram 20, preformed hole 21, grid hole
22。
Specific embodiment
In order to make those skilled in the art more fully understand the present invention program, with reference to the accompanying drawing and it is embodied
The present invention is described in further detail for mode.
Please refer to figs. 1 to 4, and Fig. 1 is the structural schematic diagram of test reaction unit provided by the embodiment of the present invention；Fig. 2
For the structural schematic diagram figure of geotextiles provided by the embodiment of the present invention；Fig. 3 is protection support lattice provided by the embodiment of the present invention
The structural schematic diagram figure of disk；Fig. 4 is the structural schematic diagram of slip casting uniform device provided by the embodiment of the present invention.
Test reaction unit provided in an embodiment of the present invention regulates and controls lower microorganism grouting and reinforcing sand for organic substrate
Body, including hold-up vessel 1, first pump housing 2, second pump housing 16, connecting pipe 3, reaction vessel and by-pass line 6, wherein
Support device is provided in reaction vessel, support device can be protection support lattice disk 5, and support device offers leakage
Hole, i.e. grid hole 22 are placed with sand body 4 in support device,
The top of reaction vessel offers solution grouting port and bacterium solution grouting port, and channel 12 is provided in reaction vessel, leads to
The top in road 12 is connected to solution grouting port and bacterium solution grouting port, and bottom is located at the top of sand body 4, and channel 12 includes successively
The first vertical section 9, tilting section 10 and the second vertical section 11 of connection,
The sample tap 14 and exhaust outlet 15 being connected to channel 12 are offered on reaction vessel,
It is provided with slip casting uniform device 8 in reaction vessel and between the second vertical section 11 and sand body 4,
Hold-up vessel 1 is connected to by connecting pipe 3 with solution grouting port, and first pump housing 2 is provided on connecting pipe 3,
The bottom end of reaction vessel is provided with leakage fluid dram 20, and the first water check valve 18 is provided on leakage fluid dram 20,
The top of by-pass line 6 is connected to reaction vessel, and communicating position is located at the lower section of slip casting uniform device 8, bypass pipe
The bottom end in road 6 is connected to the bottom end of reaction vessel and connectivity part is provided with the second water check valve 19.
In use, will be configured and the reaction solution including culture medium, calcium chloride and urea of disinfecting action is undergone to mix
It closes and is saved in injection hold-up vessel 1,
First pump housing 2 is opened when slip casting, after reaction solution flows through connecting pipe 3, by solution grouting port, flow channel
12, meanwhile, during injecting reaction solution, by Pasteur's gemma of the good fixed concentration of shaken cultivation in sterile working room
Bacillus bacterium solution in the first vertical section 9 and is inclined by bacterium solution grouting port, injection channel 12, Bacillus pasteurii bacterium solution and reaction solution
After the connection turning of oblique section 10 is sufficiently mixed, it is flow to slip casting uniform device 8 along the ramp of tilting section 10, is made mixed anti-
Liquid is answered uniformly to splash down the inside circumference in reaction vessel；
When reacting or sampling using immersion type, the reaction solution in reaction vessel is pumped by bypass pipe by second pump housing 16
In road 6, the second water check valve 19 is simultaneously closed off；
When using circulating reaction, then the second water check valve 19 is opened, it will be in reaction vessel by second pump housing 16
It is recycled in reaction solution sucking by-pass line 6, flow to sand body top again, make reaction solution according to following repeatedly clockwise
Circulation is dynamic, when sand body 4 vertically reinforces uneven, second pump housing 16 can be made reversely to pump, make reaction solution according to the inverse time
The flowing of needle direction；
When carrying out solution sampling, the first water check valve 18 is opened, when carrying out sand sampling, by soil sampler 13 from taking
Sample mouth 14 protrudes into.
Specifically, being equipped with geotextiles 7 on sand body 4, the preformed hole passed through for soil sampler 13 is offered in geotextiles 7
21, it is referred to as geotextiles and reserves cylindrical hole.
Wherein, first pump housing 2 is peristaltic pump, and second pump housing 16 is pressure pump.The inclination of tilting section 10 with respect to the horizontal plane
Angle is 10 °.Slip casting uniform device 8 is disk.
Wherein, reaction vessel is made of upper cover and lower case, and upper cover and lower case pass through the screw socket that is screwed on
Connection, and the junction of the two is between slip casting uniform device 8 and sand body 4.
Wherein, the glass handle 17 for rotating sand body 4 is provided on reaction vessel.It is provided with below reaction vessel
Damping device.
The embodiment of the present invention also provides a kind of test reaction method, regulates and controls lower microorganism grouting and reinforcing sand for organic substrate
The soil body, based on test reaction unit described in above-mentioned any one embodiment, comprising:
By it is configured and undergo disinfecting action including culture medium, calcium chloride and urea reaction solution mixed injection storage
It deposits and is saved in container 1,
First pump housing 2 is opened when slip casting, after reaction solution flows through connecting pipe 3, by solution grouting port, flow channel
12, meanwhile, during injecting reaction solution, by Pasteur's gemma of the good fixed concentration of shaken cultivation in sterile working room
Bacillus bacterium solution in the first vertical section 9 and is inclined by bacterium solution grouting port, injection channel 12, Bacillus pasteurii bacterium solution and reaction solution
After the connection turning of oblique section 10 is sufficiently mixed, it is flow to slip casting uniform device 8 along the ramp of tilting section 10, is made mixed anti-
Liquid is answered uniformly to splash down the inside circumference in reaction vessel；
When reacting or sampling using immersion type, the reaction solution in reaction vessel is pumped by bypass pipe by second pump housing 16
In road 6, the second water check valve 19 is simultaneously closed off；
When using circulating reaction, then the second water check valve 19 is opened, it will be in reaction vessel by second pump housing 16
It is recycled in reaction solution sucking by-pass line 6, flow to sandy soils top again, make reaction solution according to following repeatedly clockwise
Circulation is dynamic, when sand body 4 vertically reinforces uneven, second pump housing 16 can be made reversely to pump, make reaction solution according to the inverse time
The flowing of needle direction；
When carrying out solution sampling, the first water check valve 18 is opened, when carrying out sand sampling, by soil sampler 13 from taking
Sample mouth 14 protrudes into.
In order to advanced optimize above scheme, above-mentioned test reaction method further includes before starting test,
After cleaning test reaction unit, sand body 4 is sprinkled into reaction vessel, then each section of test reaction unit
The 20min that persistently sterilizes under the conditions of 121 DEG C is put into autoclave, then finishing device splicing and assembling in sterile working room,
And by the pre-configured reaction solution including culture medium, calcium chloride and urea, pour into stand-by in hold-up vessel 1.
Test reaction unit provided in an embodiment of the present invention is to make device sample more convenient, sample and reinforcing more
Uniformly, enhancement device waterproofness and guarantee to react aseptic condition, micro- life under a set of completely new organic substrate regulation thus designed
The experimental rig of object grouting and reinforcing sand body, this has extremely important scientific meaning and ginseng for sand cure test from now on
Examine value.
Test reaction unit provided in an embodiment of the present invention is a kind of easy to operate, the low organic group of manufacture, maintenance cost
Matter regulates and controls the experimental rig of lower microorganism grouting and reinforcing sand body.Test reaction unit provided in an embodiment of the present invention, which can improve, to be taken
Sample non-destructive, sample uniformity and device waterproofness, and guarantee the aseptic technique of reaction, and then improve the solidification of sand body
Effect.
Test reaction unit provided in an embodiment of the present invention mainly includes three basic structures, is slip casting structure respectively, anti-
Answer structure and drain loop structure.
Slip casting structure division, by hold-up vessel 1, peristaltic pump, peristaltic pump tube, bacterium solution slip casting lid, bacterium solution grouting port, solution
Grouting port, sampling lid, exhaust outlet 15, gas vent, slip casting homogenizer 8, slip casting homogenizer center disk, on the screw socket etc. that is screwed on it is more
A part composition, wherein peristaltic pump i.e. first pump housing 2, peristaltic pump tube, that is, connecting pipe 3, bacterium solution grouting port lid are fastened on bacterium solution
On grouting port, sample tap lid is fastened on sample tap 14, and gas vent is provided in exhaust outlet 15, in slip casting homogenizer center disk
It sets in slip casting homogenizer 8.
Reaction structure part contains down the screw socket that is screwed on, geotextiles 7, geotextiles and reserves cylindrical hole, sand body 4, glass handle
Multiple components such as hand 17, protection support lattice disk 5 and grid hole 22, wherein geotextiles reserve cylindrical hole, that is, preformed hole 21, protection support lattice
Grid hole 22 is offered on disk 5, the screw socket that is above the screwed on screw socket that is screwed on under is connect.
Drain loop structure part then contains leakage fluid dram 20, the first water check valve 18, the second water check valve 19, by-pass line
6, the component parts such as pressure pump.Pressure pump i.e. second pump housing 16.
It is infused in use, the reaction solutions such as culture medium, calcium chloride and urea configured and that undergo disinfecting action are mixed
Enter and saved in hold-up vessel 1, when slip casting opens peristaltic pump, after reaction solution flows through peristaltic pump tube, by solution grouting port, stream
Enter slip casting inside configuration；During injecting the reaction solutions such as culture medium, bacterium solution slip casting lid is opened, in sterile working room
By the Bacillus pasteurii bacterium solution of the good fixed concentration of shaken cultivation by bacterium solution grouting port, slip casting inside configuration is finally injected,
After being sufficiently mixed the reaction solutions such as itself and culture medium at the main body section turning in channel, the ramp for being 10 ° along gradient
It is flow to the slip casting homogenizer center disk position on slip casting homogenizer, splashes down mixed reaction solution uniformly four inside container
Reach uniform on sand reinforcing in-plane in week, the screw socket that is screwed on plays the work for being linked together slip casting structure and reaction structure
With.The whole height of entire slip casting structure is 372mm, transverse width 786mm.
In reaction structure, is highly covered for 4 top of sand body of 150mm by geotextiles 7, prevent reaction solution from splashing
4 surface of sand body is washed away during falling, so that leading to sand surface irregularity.
And preformed hole 54 is then reserved in geotextiles 7, facilitate soil sampler 13 to be downwardly into sand body 4 from sample tap 14
Portion's original state sampling.
Uniformly it is covered with the grid hole 122 of 10 × 10mm on the protection support lattice disk 5 of reaction structure lower end, primarily serves two aspects
Effect: first is that the sand body 4 to top is played a supporting role, sand body is prevented to be squashed；It on the other hand is to flow through in sand body 4
The reaction solution free seepage in portion is into drain loop structure.
Glass handle 17 is then that sand body 4 is constantly conveniently rotated in sampling process, is downwardly into sand using soil sampler 13
In the soil body 4, regular discontinuity sampling.The screw socket that is screwed at reaction structure position up and down is by the slip casting structure on top and the drain of lower part
For loop structure organic linking at an entirety, the whole height of reaction structure is 250mm, and main body container diameter is 300mm, glass
Handle width is 70mm.
In drain loop structure, right end is discharge portion, and 20 diameter of leakage fluid dram is 20mm, and inclination angle is 60 °, and length is
90mm.It is provided with the first water check valve 18 in the middle part of leakage fluid dram 20, drain is facilitated to sample and change liquid.
Damping device is provided with below reaction vessel, damping device can be rubber pad, and rubber pad plays support, damping
Effect keeps whole device body to be stabilized on experiment porch.
The left end of drain loop structure is cyclic part, is equipped with the second sealing in the critical part of cyclic part and discharge portion
Valve 19, when being reacted or being sampled using immersion type, is reacted right end using pressure pump for controlling circulating for reaction solution
Liquid is pumped into the loop structure of left end, simultaneously closes off the second water check valve 19；When using circulating reaction, then by the second water check valve
19 open, and flow to right end sandy soils top again in reaction solution suction pressure pump line by pressure pump, by controlling pressure
The purge rate of pump flows reaction solution according to clockwise direction iterative cycles；But when sand body 4 is vertically reinforced unevenly
When, pressure pump can be made to generate opposite effect power, flow reaction solution according to counter clockwise direction, guarantee sand body 4 perpendicular with this
Upward uniform consolidation, the whole height of cyclic part is 425mm, diameter 120mm in drain loop structure, and drainage structure
Whole height is 110mm, diameter 300mm.
Test reaction unit provided in an embodiment of the present invention is PC material, can according to the size of actually required sand column sample and
Quantity carrys out the internal diameter and height of determining device.Organic substrate provided by the present invention regulates and controls lower microorganism grouting and reinforcing sand body
Reaction unit is tested, is had the advantage that
1, device high temperature resistant and corrosion resistance are preferable.PC material used by the present apparatus is resistant to 200 DEG C or so high temperature, can protect
Card device is sufficiently sterilized in autoclave using preceding, while PC material is avoided that and weak erosive solution reaction.With silicon boride glass
Most of materials such as glass are compared, and the overall weight of reaction unit made of PC material is lighter, easy to carry, and cost compared with
It is low；
2, the waterproofness of device is preferable.Whole device is divided into slip casting structure, reaction structure and drain loop structure etc. three
Part is mutually spliced by the screw socket that is screwed between each structure, and such setting method can avoid occurring to use in conventional method
Bolt bolt mouth wear phenomenon as caused by being used for a long time on gas leakage drainage and ring flange, has caused by ring flange
Preferable splicing globality；
3, the defect for reinforcing occurred reaction solution static state in traditional sand body bracing means using immersion type is overcome, and
Using the seepage state of capillary water in sand body under circulating reinforcing energy real simulation natural conditions, and the device is closing the
When two water check valves 19, immersion type reinforcing can be equally carried out；
4, device can guarantee the uniformity of sand soil stabilization in use, be mainly reflected in uniform plane reinforce and
Two aspects of vertical uniform consolidation.In terms of uniform plane reinforcing, the reaction solutions such as culture medium, calcium chloride, urea pass through solution
Inside grouting port injection device, at the same time, bacterium solution is also poured into slip casting structure by bacterium solution grouting port, and two kinds of solution are being infused first
The turning of slurry main structure uniformly mixes, and then flow to slip casting homogenizer center disk position along the ramp that gradient is 10 °,
Mixed reaction solution will splash down around from center disk at this time, and uniformly flow to geotextiles 7 upper surface and slowly to
Lower fair current, thus can the uniform consolidation of basic guarantee solution in the plane；In terms of vertical uniform consolidation, pressure pump is mainly utilized
The cycle rate for adjusting reaction solution, when vertically water penetration is poor for sand body 4, adjustable pressure pump generates it reversely
Pressure, enables reaction solution counterclockwise flow, i.e. reaction solution from bottom to top reversely reinforces sand body 4.This measure is also engineering reality
Border provides certain thinking in reinforcing, and ensures whole reinforcing uniformity from plane and vertical two angles；
5, whole device aeration condition is preferable.Since the living necessities of Bacillus pasteurii in experiment reaction process are a certain amount of
Oxygen the gases such as removing carbon dioxide, exhaust outlet 15 thus set are able to satisfy this demand side by side.When container air pressure inside is big
When external pressure, the gases such as carbon dioxide generated inside container can be discharged in time；When container interal supplying oxygen deficiency, to
After internal waste gas discharge, outside air can enter inside container, oxygen needed for providing Bacillus pasteurii vital movement, thus
Bacterial activity is kept, finally ensures consolidation effect；
6, present apparatus sampling is convenient, and can guarantee in-situ sampling, can reduce experimental period.When carrying out solution sampling, beat
The first water check valve 18 is opened, the first water check valve 18 is closed after to be sampled；When carrying out the sampling of sand column, pressure pump is opened, it will be right
End reaction solution is evacuated in the loop structure of left end, is then turned off the second water check valve 19 and pressure pump, is then opened sampling lid, is utilized
Undisturbed soil soil sampler 13 is directed at geotextiles and reserves cylindrical hole, is vertically quickly downwardly into, to 13 nib contacts of soil sampler to bottom
Protection support lattice disk 5 when, stop downwards cut simultaneously soil sampler 13 is lifted, sand is squeezed out, close sampling lid, according to reality
Required sand pillar height degree is cut.Similarly, when lower sub-sampling, using 17 revolving reaction structure of glass handle, alignment is another
One geotextiles reserve cylindrical hole, cut downwards and take out, until completing all sampling operations.If measure the item is more, can increase
One entire sand column is divided into several after sampling, is accordingly detected, such sampling mode both ensure that sand column by sand body height
Original state, the drawbacks of overcoming the mode of conventionally employed pre-buried iron hoop net, and capable of periodically being sampled in same device, avoid because
A large amount of control groups are set and extend experimental period.
In conclusion test reaction unit provided in an embodiment of the present invention production is simple, cost is relatively low, strong applicability, can
Reuse, sampling is convenient, it is uniform to reinforce, ventilates preferably, and waterproofness, heat-resisting quantity and corrosion resistance are preferable, can real simulation
Reinforcing state under natural conditions, can reduce significantly experimental period, and then guarantee good sand consolidation effect.
The test reaction unit that organic substrate provided in an embodiment of the present invention regulates and controls lower microorganism grouting and reinforcing sand body makes
Specific step is as follows:
1, after cleaning reaction unit, sand body 4 is sprinkled into reaction structure, then device each section is put into autoclave
Persistently sterilize 20min under the conditions of 121 DEG C, then finishing device splicing and assembling in sterile working room, will be pre-configured
The reaction solutions such as culture medium, calcium chloride, pour into stand-by in the reaction solutions such as culture medium hold-up vessel 1, close the first water check valve 18,
Open the second water check valve 19；
2, peristaltic pump is opened, the reaction solutions such as injection culture medium open simultaneously bacterium solution slip casting lid, inject Bacillus pasteurii
Bacterium solution is uniformly injected into sand body to reaction mixture, then opens pressure pump, flows reaction solution according to clockwise direction；
3, the first water check valve 18 is every other day opened, pressure pump is closed, after liquid in device is drained, closes the first sealing
Valve 18, according to above-mentioned steps 1~2 again slip casting, drain；
4, right end reaction solution is pumped into the loop structure of left end before sampling, sampling lid is opened when sampling, by soil sampler
13 alignment geotextiles reserve cylindrical hole, are inserted perpendicularly into sand inside soil body to 5 top of protection support lattice disk, then take out sample, squeeze
It is depressed on platform and sand column is cut according to required size, finally sample is tested and analyzed；
5, it is periodically sampled according to requirement of experiment, after rotary glass handle, prepares sand column sample according to step 4；
6, reaction solution is discharged after reaction for experiment, and the sand sample after taking out remaining reinforce cleans each section component,
New round experiment is carried out according to step 1~5.
Organic substrate provided by the invention regulates and controls the test reaction unit of lower microorganism grouting and reinforcing sand body, be pass through to
The nutrient solutions such as organic substrates, bacterium solution, culture medium, urea and the calcium sources such as bovine serum albumin(BSA) are inculcated in sand body, utilize generation
The hole of sand body is filled and is gelled by calcarinate body, so as to improve its physical and mechanical property, is finally reached sand
The purpose that body is reinforced.
Experiment can be reacted according to specific requirements slip casting, and such as every other day slip casting is primary, and be discharged once by leakage fluid dram,
(parameters such as actual device size, slurries injection rate, sand height, sand partial size can be according to practical need by i.e. " one day one row of a note "
To determine), after sand body reaches slip casting reaction time, periodically take out solidification sand column sample, finally wait react at the end of arrange
Reaction solution to the greatest extent, and remove experimental provision component and be cleaned and dried, for future use.
Test reaction unit provided in an embodiment of the present invention is when in use:
Sand weight has been weighed in advance, quality error places geotextiles within the scope of ± 5g, on 5 top of protection support lattice disk,
Sand is sprinkled into geotextiles top, shows to repave one layer of geotextiles 7 in sand, each section device assembly is placed in autoclave and is gone out
Bacterium, finishing device connects in sterile working room after sterilizing.
The device that connects is taken out, by the culture medium of configured 5.52L, the urea of 2.76L, 0.5mol/L, 2.76L,
The CaCl2 mixing of the bovine serum albumin(BSA) that mass fraction is 0.15%, 2.76L, 0.5mol/L is added in hold-up vessel 1, setting
Initial pH is 7.3, then turns on peristaltic pump, makes inside mixed liquor inflow device, opens simultaneously bacterium solution grouting port, and injection is initial
OD600=1.0, volume 0.345L Bacillus pasteurii bacterium solution, close peristaltic pump after evenly mixing, cover slip casting lid.Then
Pressure pump is opened, reaction solution uniform slip casting along clockwise direction is made.
Daily one note of a row takes solution example to detect relevant ions concentration, opens sampling every three days every two hours
Mouth 14, rotary glass handle 17 enable the soil sampler 13 that diameter is 50mm be directed at geotextiles preformed hole, downwards vertically incision rapidly,
It reaches and is proposed upwards after formulating depth, sample is squeezed to test platform, cut sand column with native knife is cut, obtain desired height
Sand sample is detected for indices.
After experiment, the separation of each section component is cleaned, it is dry, it is used to the later period.
Used herein a specific example illustrates the principle and implementation of the invention, and above embodiments are said
It is bright to be merely used to help understand method and its core concept of the invention.It should be pointed out that for the ordinary skill of the art
, without departing from the principle of the present invention, can be with several improvements and modifications are made to the present invention for personnel, these improvement
It is also fallen within the protection scope of the claims of the present invention with modification.
Claims (10)
1. a kind of test reaction unit regulates and controls lower microorganism grouting and reinforcing sand body for organic substrate, which is characterized in that including
Hold-up vessel, first pump housing, second pump housing, connecting pipe, reaction vessel and by-pass line, wherein
Support device is provided in the reaction vessel, the support device offers leak hole, is placed in the support device
Sand body,
The top of the reaction vessel offers solution grouting port and bacterium solution grouting port, is provided with channel in the reaction vessel,
The top in the channel is connected to the solution grouting port and the bacterium solution grouting port, and bottom is located at the upper of the sand body
Side, the channel include the first vertical section being sequentially communicated, tilting section and the second vertical section,
The sample tap and exhaust outlet being connected to the channel are offered on the reaction vessel,
It is provided with slip casting uniform device in the reaction vessel and between second vertical section and the sand body,
The hold-up vessel is connected to by the connecting pipe with the solution grouting port, is provided on the connecting pipe described
First pump housing,
The bottom end of the reaction vessel is provided with leakage fluid dram, and the first water check valve is provided on the leakage fluid dram,
The top of the by-pass line is connected to the reaction vessel, and communicating position is located at the lower section of the slip casting uniform device,
The bottom end of the by-pass line is connected to the bottom end of the reaction vessel and connectivity part is provided with the second water check valve.
2. test reaction unit according to claim 1, which is characterized in that be equipped with geotextiles on the sand body, institute
State the preformed hole for offering in geotextiles and passing through for soil sampler.
3. test reaction unit according to claim 1, which is characterized in that the support device is protection support lattice disk.
4. test reaction unit according to claim 1, which is characterized in that first pump housing is peristaltic pump, described the
Two pump housings are pressure pump.
5. test reaction unit according to claim 1, which is characterized in that the inclination of the tilting section with respect to the horizontal plane
Angle is 10 °.
6. test reaction unit according to claim 1, which is characterized in that the slip casting uniform device is disk.
7. test reaction unit according to claim 1, which is characterized in that the reaction vessel is by upper cover and lower part
Shell composition, the upper cover is connected with the lower case by the screw socket that is screwed on, and the junction of the two is located at the note
It starches between uniform device and the sand body.
8. test reaction unit according to claim 1, which is characterized in that be provided on the reaction vessel for rotating
The glass handle of the sand body.
9. a kind of test reaction method regulates and controls lower microorganism grouting and reinforcing sand body for organic substrate, which is characterized in that be based on
Test reaction unit described in the claims 1-9 any one, comprising:
It will be configured and undergo storage described in the reaction solution mixed injection including culture medium, calcium chloride and urea of disinfecting action
It deposits in container and saves,
First pump housing is opened when slip casting, after the reaction solution flows through connecting pipe, by solution grouting port, is flowed into described logical
Road, meanwhile, during injecting the reaction solution, by the Pasteur of the good fixed concentration of shaken cultivation in sterile working room
Bacillus bacterium solution injects the channel by bacterium solution grouting port, and the Bacillus pasteurii bacterium solution and the reaction solution exist
After the connection turning of first vertical section and the tilting section is sufficiently mixed, it is flow to along the ramp of the tilting section described
Slip casting uniform device makes mixed reaction solution uniformly splash down the inside circumference in the reaction vessel；
It, will be described in the reaction solution suction in the reaction vessel by second pump housing when being reacted or being sampled using immersion type
In by-pass line, second water check valve is simultaneously closed off；
When using circulating reaction, then will second water check valve opening, by second pump housing by the reaction vessel
In reaction solution suck in the by-pass line and recycled, flow to the sandy soils top again, make reaction solution according to up time
The flowing of needle direction iterative cycles, when the sand body vertically reinforces uneven, can be such that second pump housing reversely pumps,
Flow reaction solution according to counter clockwise direction；
When carrying out solution sampling, opens first water check valve and take soil sampler from described when carrying out sand sampling
Sample mouth protrudes into.
10. test reaction method according to claim 9, which is characterized in that further include before starting test,
After cleaning the test reaction unit, the sand body is sprinkled into the reaction vessel, then the test is reacted
Each section of device is put into autoclave the 20min that persistently sterilizes under the conditions of 121 DEG C, then the finishing device in sterile working room
Splicing and assembling, and by the pre-configured reaction solution including culture medium, calcium chloride and urea, pour into the hold-up vessel
It is interior stand-by.
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| CN112858019A (en) * | 2021-01-08 | 2021-05-28 | 合肥工业大学 | Test device and test method for repairing air pressure fracture by MICP technology |
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Application publication date: 20190802 |