CN112852904A - Treatment method of plant essential oil/hydrolat processing wastewater - Google Patents
Treatment method of plant essential oil/hydrolat processing wastewater Download PDFInfo
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- CN112852904A CN112852904A CN202110128111.0A CN202110128111A CN112852904A CN 112852904 A CN112852904 A CN 112852904A CN 202110128111 A CN202110128111 A CN 202110128111A CN 112852904 A CN112852904 A CN 112852904A
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- hydrolat
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention relates to the technical field of industrial wastewater treatment and resource utilization, and provides a method for treating plant essential oil/hydrolat processing wastewater. The invention develops a novel treatment method of plant essential oil/hydrolat processing wastewater, and creatively provides a method for preparing microbial polysaccharide by taking the plant essential oil/hydrolat processing wastewater as a fermentation raw material of polysaccharide-producing microorganisms. Through the metabolism of polysaccharide-producing microorganisms, organic substances in the plant essential oil/hydrolat processing wastewater can be decomposed, and fermentation products of the plant essential oil/hydrolat processing wastewater can be utilized by people, so that the utilization rate of wastewater resources is improved, and the plant essential oil/hydrolat processing wastewater is green and environment-friendly and has good economic and ecological benefits.
Description
Technical Field
The invention relates to the technical field of industrial wastewater treatment and resource utilization, in particular to a plant essential oil/hydrolat processing wastewater treatment method.
Background
Essential oils are volatile aromatic substances extracted from different parts of plants, such as flowers, leaves, roots, seeds, fruits, etc. Steam distillation is the most common essential oil extraction method used in most essential oil manufacturing industries. The liquid product obtained by steam distillation is divided into an upper layer and a lower layer, the upper layer is essential oil, the lower layer is hydrolat, and distillation residual liquid rich in water-soluble organic matters, namely plant essential oil/hydrolat processing wastewater, is remained in a distillation kettle after distillation. Plant essential oil/hydrolat belongs to one of plant extracts, and the treatment method of waste water in the production process of the plant extracts is mainly divided into a chemical method, a mass transfer method and a biological treatment method at present, wherein the most widely applied method is the biological treatment method, and the method is a process of oxidizing organic matters which are abundantly existed in the waste water into stable inorganic matters through the metabolism of microorganisms and then discharging or recycling the stable inorganic matters. The biological treatment method in the prior art has the problems of complex process, need of a specific device, need of adding a large amount of additives, easy odor emission, breeding of harmful bacteria and mosquitoes and flies, bad sanitary conditions and the like. For example, chinese patent publication No. CN106495411A discloses an ICBB process and an apparatus for wastewater treatment in the extraction process of ginkgo biloba extract, and the provided ICBB process for wastewater treatment in the extraction process of ginkgo biloba extract comprises the steps of homogenization treatment, DSIC coagulating sedimentation treatment, AOPs advanced oxidation treatment, DSBB biological bacteria treatment, anaerobic treatment, hydrolytic acidification treatment, aerobic treatment, BAF biological aerated filter treatment, DS terminal treatment, and the like. The process is complicated, a large amount of coagulant and alkalizer are required to be added, and the alkalinity or hardness of the discharged water body is possibly too high, so that the growth of organisms such as fish, shrimps and the like in the water is not facilitated. The plant essential oil/hydrolat processing wastewater has complex components, not only contains various aromatic compounds, but also contains a large amount of natural organic substances, such as: protein, saccharide, cellulose, hemicellulose and the like, and belongs to wastewater with high biodegradability. However, the plant essential oil/hydrolat processing wastewater is different from the plant extract wastewater in the conventional sense, and as the essential oil or hydrolat prepared by the steam distillation method is pure natural and has no additive, the residual components in the wastewater are almost harmless to the human body, and the research on the aspect of applying the plant essential oil/hydrolat processing wastewater to the microbial fermentation engineering is rarely carried out at present. Therefore, it is of great significance to develop a method for effectively treating essential oil/hydrolat processing wastewater and fully utilizing wastewater resources to prepare microbial fermentation products required by people.
Disclosure of Invention
The invention aims to overcome the defects and shortcomings of the prior art and provides a method for treating plant essential oil/hydrolat processing wastewater.
The above object of the present invention is achieved by the following technical solutions:
a method for treating plant essential oil/hydrolat processing wastewater is provided, which takes the plant essential oil/hydrolat processing wastewater as a fermentation raw material of polysaccharide-producing microorganism to prepare microbial polysaccharide.
The invention expands the treatment method of the plant essential oil/hydrolat processing wastewater, provides a novel method for carrying out microbial fermentation by utilizing the plant essential oil/hydrolat processing wastewater, decomposes organic substances in the plant essential oil/hydrolat processing wastewater through the metabolism of polysaccharide-producing microbes, and can be utilized by people after a fermentation product is separated from a water body, thereby improving the utilization rate of wastewater resources.
The method comprises the following steps:
s1, inoculating polysaccharide-producing microorganisms into plant essential oil/hydrolat processing wastewater, and carrying out aerobic fermentation;
s2, carrying out solid-liquid separation after fermentation to obtain a supernatant rich in microbial polysaccharides;
and S3, purifying the supernatant, standing for 1-12 h, and carrying out solid-liquid separation to obtain the microbial polysaccharide.
Preferably, the plant essential oil/hydrolat process wastewater is used as the sole fermentation feedstock for the polysaccharide producing microorganism.
Preferably, the plant essential oil/hydrolat processing wastewater is wastewater generated after one or more of rose, chamomile in Germany and tea tree in Australia are prepared into essential oil/hydrolat by a steam distillation method.
Preferably, the polysaccharide-producing microorganism of step S1 is xanthomonas campestris or sphingomonas paucimobilis.
Preferably, the inoculation amount of the polysaccharide-producing microorganism in the step S1 is 2.5-15%.
Preferably, the fermentation time of step S1 is 2-7 days, and the fermentation temperature is 25-35 ℃.
Preferably, the solid-liquid separation mode in step S2 is centrifugation or filtration.
Preferably, when the polysaccharide-producing microorganism is sphingomonas paucimobilis, after the fermentation is finished, the fermentation liquid is heated to 90-100 ℃, and is subjected to heat preservation for 15-30 min, and then solid-liquid separation is performed.
Preferably, in the step S3, the purification treatment is to add a solvent with a volume ratio of 1-4: 1 or adjusting the pH value of the supernatant to 1.0-3.0 to precipitate the microbial polysaccharide from the supernatant.
Preferably, when the polysaccharide-producing microorganism is sphingomonas paucimobilis, the purification treatment in step S3 is to adjust the pH of the supernatant to 10.0-11.0 for 10-15 min, then adjust the pH of the supernatant to 6.0-7.0, and add the supernatant in a volume ratio of 1-4: 1 of anhydrous ethanol.
Preferably, when the polysaccharide-producing microorganism is Sphingomonas paucimobilis, the purification treatment of step S3 is to adjust the pH of the supernatant to 10.0-11.0, treat for 10-15 min, and then adjust the pH of the supernatant to 1.0-3.0.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for treating plant essential oil/hydrolat processing wastewater, which creatively provides that the plant essential oil/hydrolat processing wastewater is used as a fermentation raw material of polysaccharide-producing microorganisms to prepare microbial polysaccharides. The metabolism of the polysaccharide-producing microorganism can decompose organic substances in plant essential oil/hydrolat processing wastewater, and a fermentation product of the polysaccharide-producing microorganism can be utilized by people, so that the utilization rate of wastewater resources is improved, and the polysaccharide-producing microorganism is green and environment-friendly and has good economic and ecological benefits.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
EXAMPLE 1 treatment of Rose essential oil/hydrolat processing wastewater
Inoculating xanthomonas campestris into rose essential oil/hydrolat processing wastewater with an inoculum size of 15%, carrying out aerobic fermentation at 25 ℃ for 7 days, filtering fermentation liquor after the fermentation is finished, carrying out solid-liquid separation to obtain supernatant, adding absolute ethyl alcohol into the supernatant, wherein the volume ratio of the supernatant to the absolute ethyl alcohol is 1:1, standing for 12 hours, and centrifuging to obtain a xanthan gum product, wherein the yield is 3.0 g/L.
Example 2 treatment of German Chamomile essential oil/hydrolat processing wastewater
Inoculating sphingosine paucimobilis into German chamomile essential oil/hydrolat processing wastewater with an inoculation amount of 10%, aerobically fermenting for 5 days at 35 ℃, heating fermentation liquor to 90 ℃ after fermentation is finished, preserving heat for 30 minutes, centrifuging, separating to obtain supernatant, adding absolute ethyl alcohol into the supernatant, keeping the volume ratio of the supernatant to the absolute ethyl alcohol at 1:2, standing for 4 hours, filtering to obtain a high-acyl gellan gum product, wherein the yield is 4.5 g/L.
Example 3 treatment of German Chamomile essential oil/hydrolat processing wastewater
Inoculating sphingomonas paucimobilis into German chamomile essential oil/hydrolat processing wastewater with an inoculation amount of 2.5%, carrying out aerobic fermentation for 3 days at 30 ℃, heating a fermentation liquid to 100 ℃ after the fermentation is finished, preserving heat for 15 minutes, filtering to obtain a supernatant, cooling the supernatant, adjusting the pH of the supernatant to 10.0 by using a sodium hydroxide aqueous solution, treating the supernatant at 80 ℃ for 10 minutes, adjusting the pH to 7.0 by using a hydrochloric acid aqueous solution, adding absolute ethyl alcohol with a volume ratio of 1:3, standing for 1 hour, and carrying out centrifugal solid-liquid separation to obtain a low-acyl gellan gum product with the yield of 2.5 g/L.
Example 4 treatment of Australian tea Tree essential oil/hydrolat processing wastewater
Inoculating Xanthomonas campestris to Australia tea tree essential oil/hydrolat processing wastewater with an inoculation amount of 5%, carrying out aerobic fermentation for 2 days at 30 ℃, carrying out centrifugal treatment on fermentation liquor after the fermentation is finished, carrying out solid-liquid separation to obtain supernatant, adding absolute ethyl alcohol into the supernatant, wherein the volume ratio of the supernatant to the absolute ethyl alcohol is 1:2, standing for 1 hour, and obtaining a xanthan gum product after centrifugal solid-liquid separation, wherein the yield is 1.2 g/L.
EXAMPLE 5 treatment of two essential oil/hydrolat process wastewaters
Inoculating xanthomonas campestris into mixed essential oil/hydrolat processing wastewater of Australian tea tree and German chamomile in an inoculation amount of 5%, carrying out aerobic fermentation for 4 days at 30 ℃, carrying out centrifugal treatment on fermentation liquor after the fermentation is finished, carrying out solid-liquid separation to obtain supernatant, adjusting the pH of the supernatant to 3.0 by using a hydrochloric acid aqueous solution, standing for 1 hour, and obtaining a xanthan gum product after centrifugal solid-liquid separation, wherein the yield is 1.5 g/L.
EXAMPLE 6 treatment of three essential oil/hydrolat process wastewaters
Inoculating sphingomonas paucimobilis into Australia tea tree, German chamomile and rose mixed essential oil/hydrolat processing wastewater with an inoculation amount of 10%, carrying out aerobic fermentation for 5 days at 30 ℃, heating fermentation liquor to 95 ℃ after the fermentation is finished, carrying out heat preservation for 30 minutes, centrifuging, separating to obtain supernatant, adjusting the pH of the supernatant to 1.0 by using a hydrochloric acid aqueous solution, standing for 4 hours, and filtering to obtain a high-acyl gellan gum product with the yield of 2.5 g/L.
Claims (10)
1. A method for treating plant essential oil/hydrolat processing wastewater is characterized in that the plant essential oil/hydrolat processing wastewater is used as a fermentation raw material of polysaccharide-producing microorganisms to prepare microbial polysaccharides.
2. Method according to claim 1, characterized in that it comprises the following steps:
s1, inoculating polysaccharide-producing microorganisms into plant essential oil/hydrolat processing wastewater, and carrying out aerobic fermentation;
s2, carrying out solid-liquid separation after fermentation to obtain a supernatant rich in microbial polysaccharides;
and S3, purifying the supernatant, standing for 1-12 h, and carrying out solid-liquid separation to obtain the microbial polysaccharide.
3. The method of claim 2, wherein plant essential oil/hydrolat process wastewater is used as the sole fermentation feedstock for the polysaccharide-producing microorganism.
4. The method as claimed in claim 2, wherein the plant essential oil/hydrolat processing wastewater is wastewater generated after preparing essential oil/hydrolat by steam distillation method from any one or more of flos Rosae Rugosae, Matricaria chamomilla and tea tree of Australia.
5. The method of claim 2, wherein the polysaccharide producing microorganism of step S1 is Xanthomonas campestris or Sphingomonas paucimobilis.
6. The method according to claim 2, wherein the polysaccharide-producing microorganism is inoculated in an amount of 2.5 to 15% in step S1.
7. The method according to claim 2, wherein the fermentation time of step S1 is 2-7 days, and the fermentation temperature is 25-35 ℃.
8. The method according to claim 2, wherein the solid-liquid separation in step S2 is centrifugation or filtration.
9. The method according to claim 2, wherein when the polysaccharide-producing microorganism is Sphingomonas paucimobilis, the fermentation broth is heated to 90-100 ℃ after the fermentation is finished, and the temperature is maintained for 15-30 min, and then solid-liquid separation is performed.
10. The method according to claim 2, wherein the purification treatment of step S3 is adding a solvent in a volume ratio of 1-4: 1 or adjusting the pH value of the supernatant to 1.0-3.0 to precipitate the microbial polysaccharide from the supernatant.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH069703A (en) * | 1992-06-23 | 1994-01-18 | Shimizu Corp | Method of microbiologically producing cellulose and method of treating wastewater or waste |
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2021
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Patent Citations (6)
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|---|---|---|---|---|
| JPH069703A (en) * | 1992-06-23 | 1994-01-18 | Shimizu Corp | Method of microbiologically producing cellulose and method of treating wastewater or waste |
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| US20110027838A1 (en) * | 2009-07-31 | 2011-02-03 | Harding Nancy E | Sphingomonas Strains Producing Greatly Increased Yield Of PHB-Deficient Sphingan (Diutan) |
| CN103031262A (en) * | 2012-12-14 | 2013-04-10 | 浙江大学 | Paenibacillus sp. strain and microbial exopolysaccharide flocculant generated by same as well as application of flocculant |
| WO2020086405A1 (en) * | 2018-10-22 | 2020-04-30 | Locus Ip Company, Llc | Customizable approaches to waste water treatment |
| CN109884220A (en) * | 2019-01-15 | 2019-06-14 | 浙江工业大学 | Method for analyzing aromatic compounds in natural plant hydrolat |
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