CN112845328A - Titanium sheet flushing device for cell biology research - Google Patents
Titanium sheet flushing device for cell biology research Download PDFInfo
- Publication number
- CN112845328A CN112845328A CN202110310855.4A CN202110310855A CN112845328A CN 112845328 A CN112845328 A CN 112845328A CN 202110310855 A CN202110310855 A CN 202110310855A CN 112845328 A CN112845328 A CN 112845328A
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- China
- Prior art keywords
- titanium sheet
- titanium
- water
- sheet placing
- bottom plate
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- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 title claims abstract description 95
- 239000010936 titanium Substances 0.000 title claims abstract description 95
- 229910052719 titanium Inorganic materials 0.000 title claims abstract description 95
- 238000011160 research Methods 0.000 title claims abstract description 33
- 238000011010 flushing procedure Methods 0.000 title claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 73
- 238000005192 partition Methods 0.000 claims abstract description 15
- 238000005406 washing Methods 0.000 claims description 27
- 239000000463 material Substances 0.000 claims description 7
- 238000003860 storage Methods 0.000 claims description 7
- 239000002071 nanotube Substances 0.000 abstract description 9
- 239000000126 substance Substances 0.000 abstract description 7
- 231100000433 cytotoxic Toxicity 0.000 abstract description 6
- 230000001472 cytotoxic effect Effects 0.000 abstract description 6
- 238000003491 array Methods 0.000 abstract description 3
- 230000007321 biological mechanism Effects 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 17
- 238000012986 modification Methods 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 210000000988 bone and bone Anatomy 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- DDFHBQSCUXNBSA-UHFFFAOYSA-N 5-(5-carboxythiophen-2-yl)thiophene-2-carboxylic acid Chemical compound S1C(C(=O)O)=CC=C1C1=CC=C(C(O)=O)S1 DDFHBQSCUXNBSA-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000010883 osseointegration Methods 0.000 description 1
- 230000009818 osteogenic differentiation Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 150000003608 titanium Chemical class 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
- B08B3/12—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration by sonic or ultrasonic vibrations
Abstract
The invention discloses a titanium sheet flushing device for cell biology research, which comprises a reservoir, titanium sheet placing racks and staggered plates, wherein n running water flushing gaps are sequentially and uniformly arranged on a bottom plate of the reservoir from left to right at intervals; the periphery of the lower surface of the bottom plate of the reservoir protrudes downwards to form a rectangular clamping groove; a plurality of water outlet holes are formed in the bottom plate of the titanium sheet placing frame, and n +1 partition plates are sequentially and uniformly arranged on the bottom plate of the titanium sheet placing frame from left to right at intervals; a titanium sheet placing groove is formed between every two adjacent partition plates, and n titanium sheet placing grooves are formed between n +1 partition plates; the rectangular clamping groove is sleeved outside the top end of the titanium sheet placing frame; n water flowing gaps are arranged on the staggered plates at intervals from left to right. The titanium sheet flushing device for cell biology research is suitable for long-term flowing water flushing of titanium sheets with prepared nanotube arrays, so that cytotoxic substances are removed, and the subsequent research on biological mechanisms is facilitated.
Description
Technical Field
The invention relates to a titanium sheet flushing device for cell biology research.
Background
Titanium is one of the most widely used metal materials for clinical implants, and is mainly used for manufacturing bone defect fillers caused by various reasons at present. Despite its low elastic modulus, high strength, fatigue resistance, corrosion resistance and good biocompatibility, titanium still faces the clinical problem of aseptic loosening caused by poor osseointegration after implantation of the endoprosthesis. To solve this problem, most of the current research on promoting the osteointegration of endophytes is directed to changing the material composition, pore structure, porosity and surface coating. In recent years, nano surface modification technology has been gradually developed, which simulates the microstructure niche where cells in bone tissues are located. Research shows that the nanotube array prepared on the surface of titanium metal can independently regulate and control osteogenic differentiation of Bone Mesenchymal Stem Cells (BMSCs) under the condition of not depending on chemical factors. The research result suggests that the nano surface modification technology can be used for guiding the improved design of optimizing the plant in clinic so as to help promote the osteointegration of the interface of the plant and the host bone.
However, the existing research has not yet elucidated that the mechanism by which the nano surface modification technology regulates the directional differentiation of BMSCs is not well designed from the mechanism layer. In order to study and clarify the mechanism, a nano surface modification technology is needed to carry out biological research on the differentiation mechanism of the BMSCs. The most widely and most proposed nano surface modification technology is to prepare titanium nanotube arrays on the surface of titanium metal, wherein the most common preparation method is an anodic oxidation method. The method is simple to operate and low in instrument requirement, and researchers can prepare the method for subsequent biological research without basic knowledge of materials in a biological laboratory.
However, the nanotube array prepared by the anodic oxidation method has a certain amount of residual cytotoxic substances, mainly ammonium fluoride in the anodic oxidation electrolyte. If the removal is carried out by chemical methods, other cytotoxic elements are very easily introduced. Therefore, soaking with organic solvent and washing with flowing water for a long time are effective. However, no specific device is available at present, which can provide the nanotube modified titanium sheet prepared in a biological laboratory for long-term flowing water washing so as to achieve the purposes of removing cytotoxic substances and smoothly performing subsequent cell biology experiments.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a titanium sheet flushing device for cell biology research, which is suitable for the long-term flowing water flushing of titanium sheets with prepared nanotube arrays, so that cytotoxic substances are removed, and the subsequent research on biological mechanisms is facilitated.
The technical scheme for realizing the purpose is as follows: a titanium sheet washing unit for cell biology research, includes cistern, titanium sheet rack and crisscross board, wherein:
the water storage tank is of a cuboid structure with an opening at the upper end, and n running water flushing gaps are sequentially and uniformly arranged on a bottom plate of the water storage tank from left to right at intervals; the periphery of the lower surface of the bottom plate of the water storage tank protrudes downwards to form a rectangular clamping groove;
the titanium sheet placing frame is of a cuboid structure with an opening at the upper end, a bottom plate of the titanium sheet placing frame is provided with a plurality of water outlet holes, and n +1 partition plates are sequentially and uniformly arranged on the bottom plate of the titanium sheet placing frame from left to right at intervals; a titanium sheet placing groove is formed between every two adjacent partition plates, and n titanium sheet placing grooves are formed between n +1 partition plates;
the rectangular clamping groove is sleeved outside the top end of the titanium sheet placing frame; the n titanium sheet placing grooves are correspondingly positioned right below the n running water washing gaps one by one;
n water flowing gaps are uniformly formed in the staggered plate from left to right at intervals; the staggered plates are arranged on the bottom plate of the reservoir, and the n running water gaps and the n running water flushing gaps are arranged in a staggered mode.
The titanium sheet flushing device for cell biology research is characterized in that the flowing water flushing gap, the titanium sheet placing groove and the flowing water gap are equal in length, and the flowing water flushing gap, the titanium sheet placing groove and the flowing water gap are equal in width.
The titanium sheet flushing device for cell biological research is characterized in that the length and width dimensions of the staggered plates are smaller than those of the inner cavity of the water reservoir.
The titanium sheet flushing device for cell biology research is characterized in that the height of the partition plate is 20 mm.
The titanium sheet washing device for cell biology research is characterized in that the wall thickness of the rectangular clamping groove is smaller than that of the water reservoir.
The titanium sheet washing device for cell biology research is characterized in that the water storage tank, the titanium sheet placing rack and the staggered plates are made of plastic materials respectively.
The titanium sheet flushing device for cell biology research has the following advantages:
(1) the structure is simple, the operation is easy, and the titanium sheet with the prepared nanotube array can be washed by running water for a long time in a biological laboratory so as to remove cytotoxic substances as much as possible;
(2) the running water flushing staggered plate is skillfully designed, and the width and the flow rate of flushing can be simply and manually controlled;
(3) the titanium sheet placing rack is suitable for the titanium sheets with different sizes to be washed by running water for a long time.
Drawings
FIG. 1 is an exploded view of a titanium plate washing device for cell biology research according to the present invention;
FIG. 2 is a half-sectional view of a titanium plate washing device for cell biology research according to the present invention;
fig. 3 is a top view of a water reservoir;
FIG. 4 is a top view of the titanium sheet placing frame;
FIG. 5 is a top view of an interleaved plate.
Detailed Description
In order that those skilled in the art will better understand the technical solution of the present invention, the following detailed description is given with reference to the accompanying drawings:
referring to fig. 1, 2, 3, 4 and 5, a titanium sheet washing apparatus for cell biology research, according to a preferred embodiment of the present invention, comprises a water reservoir 1, a titanium sheet placing frame 2 and an interleaving plate 3. And n is 10.
The reservoir 1 is a cuboid structure with an opening at the upper end and is made of plastic materials. 10 flowing water washing gaps 11 are uniformly arranged on a bottom plate of the reservoir 1 from left to right at intervals; the periphery of the lower surface of the bottom plate of the reservoir 1 protrudes downwards to form a rectangular clamping groove 12; the length of the inner cavity of the water reservoir 1 is 135mm, the width is 120mm, the height is 100mm, and the wall thickness of the water reservoir 1 is 10 mm. Every flowing water washes gap 11 long 100mm, wide 5mm, and every two adjacent flowing water washes gap 11 interval 5mm, and flowing water washes gap 11 respectively 20mm apart from the front and back lateral wall of cistern 1, and the flowing water of leftmost end washes gap 11 apart from the left side wall 10mm of cistern 1, and the flowing water of rightmost end washes gap 11 apart from the right side wall 10mm of cistern 1. The wall thickness of the rectangular clamping groove 12 is smaller than that of the water reservoir 1. The wall thickness of the rectangular clamping groove 12 is 5mm, and the height is 20 mm.
The titanium sheet placing rack 2 is of a cuboid structure with an opening at the upper end and is made of plastic materials. A plurality of water outlet holes 21 with the diameter of 3mm are formed in the bottom plate of the titanium sheet placing frame 2, so that the titanium sheet placing frame is beneficial to quick outflow of washing water. 11 partition plates 22 are uniformly arranged on the bottom plate of the titanium sheet placing frame 2 from left to right at intervals; one titanium sheet placing groove 23 is formed between every two adjacent partition plates 22, and 10 titanium sheet placing grooves 23 are formed between 11 partition plates. The titanium sheet placing rack 2 is 135mm long, 120mm wide and 120mm high, and the wall thickness of the titanium sheet placing rack 2 is 5 mm. The partition board 22 is 5mm wide and 20mm high; the titanium sheet placing groove 23 is 100mm long and 5mm wide. The distance between the titanium sheet placing groove 23 and the front and rear side walls of the titanium sheet placing rack 2 is 20mm respectively, and the distance between the titanium sheet placing groove 23 at the leftmost end and the left side wall of the titanium sheet placing rack 2 is 10 mm; the distance between the rightmost titanium sheet placing groove 23 and the right side wall of the titanium sheet placing rack 2 is 10 mm.
The rectangular clamping groove 12 is sleeved outside the top end of the titanium sheet placing frame 2; the 10 titanium sheet placing grooves 23 are correspondingly positioned right below the 10 running water washing gaps 11.
The length of the staggered plate 3 is 115mm, and the width is 110 mm; is made of plastic material. 10 water flowing gaps 31 are uniformly arranged on the staggered plate 3 from left to right at intervals; the water gap 31 is 100mm long and 5mm wide. The flow gaps 31 are 5mm from the front and rear side edges of the interleaved plate 3, the leftmost flow gap is 5mm from the left side edge of the interleaved plate 3, and the rightmost flow gap is 15mm from the right side edge of the interleaved plate 3.
The staggered plates 3 are placed on the bottom plate of the reservoir 1, and 10 running water gaps 31 and 10 running water washing gaps 11 are staggered.
The flowing water washing gap 11, the titanium sheet placing groove 23 and the flowing water gap 31 are equal in length, and the flowing water washing gap 11, the titanium sheet placing groove 23 and the flowing water gap 31 are equal in width. The size of the staggered plates 3 is smaller than the inner cavity of the reservoir 1, the reservoir 1 can be freely placed into the staggered plates 3, the structure of the flowing water gaps 31 of the staggered plates 3 is the same as that of the flowing water flushing gaps 11 of the reservoir 1, when the same gap structures are staggered, 5mm intervals among the flowing water gaps 31 cover part of the flowing water flushing gaps 11, and the width of the final flowing water flushing gaps 11 can be regulated to achieve the purpose of controlling the flow rate.
When the titanium sheet flushing device for cell biology research is used, the prepared titanium sheet with the nanotube array is washed by ultrasonic oscillation and then soaked in organic solvent alcohol overnight. On arranging the titanium piece in proper order perpendicularly in titanium piece standing groove 23 of titanium piece rack 2, 1 stands in the top of titanium piece rack 2 of cistern, places whole titanium piece washing unit in tap below, washes width and speed with the flowing water that crisscross board 3 control flow water washing gap 11. The purpose of washing the titanium sheet for a long time can be achieved by continuously injecting water, the titanium sheet can be taken out after a certain time, and the follow-up biological experiment can be carried out after ethylene oxide disinfection.
In conclusion, the titanium sheet flushing device for cell biology research can vertically facilitate the titanium sheet with the prepared nanotube array on the titanium sheet frame, and can achieve the purpose of flushing the titanium sheet for a long time by continuously injecting water above the titanium sheet flushing device, thereby facilitating the research of subsequent biological mechanisms.
It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as a limitation of the present invention, and that changes and modifications to the above described embodiments are within the scope of the claims of the present invention as long as they are within the spirit and scope of the present invention.
Claims (6)
1. The utility model provides a titanium piece washing unit for cell biology research which characterized in that includes cistern, titanium piece rack and crisscross board, wherein:
the water storage tank is of a cuboid structure with an opening at the upper end, and n running water flushing gaps are sequentially and uniformly arranged on a bottom plate of the water storage tank from left to right at intervals; the periphery of the lower surface of the bottom plate of the water storage tank protrudes downwards to form a rectangular clamping groove;
the titanium sheet placing frame is of a cuboid structure with an opening at the upper end, a bottom plate of the titanium sheet placing frame is provided with a plurality of water outlet holes, and n +1 partition plates are sequentially and uniformly arranged on the bottom plate of the titanium sheet placing frame from left to right at intervals; a titanium sheet placing groove is formed between every two adjacent partition plates, and n titanium sheet placing grooves are formed between n +1 partition plates;
the rectangular clamping groove is sleeved outside the top end of the titanium sheet placing frame; the n titanium sheet placing grooves are correspondingly positioned right below the n running water washing gaps one by one;
n water flowing gaps are uniformly formed in the staggered plate from left to right at intervals; the staggered plates are arranged on the bottom plate of the reservoir, and the n running water gaps and the n running water flushing gaps are arranged in a staggered mode.
2. The titanium plate washing device for cell biological research according to claim 1, wherein the flowing water washing slit, the titanium plate placing groove and the flowing water slit are equal in length, and the flowing water washing slit, the titanium plate placing groove and the flowing water slit are equal in width.
3. The titanium sheet washing apparatus for cell biology research according to claim 1, wherein the length and width dimensions of the interleaving plate are smaller than those of the inner cavity of the water reservoir.
4. The titanium sheet washing device for cell biological research as claimed in claim 1, wherein the height of the partition board is 20 mm.
5. The titanium sheet washing device for cell biological research according to claim 1, wherein the wall thickness of the rectangular clamping groove is smaller than that of the water reservoir.
6. The titanium sheet washing device for cell biology research according to claim 1, wherein the water reservoir, the titanium sheet placing frame and the interleaving plate are made of plastic materials respectively.
Priority Applications (1)
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CN202110310855.4A CN112845328A (en) | 2021-03-23 | 2021-03-23 | Titanium sheet flushing device for cell biology research |
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CN202110310855.4A CN112845328A (en) | 2021-03-23 | 2021-03-23 | Titanium sheet flushing device for cell biology research |
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CN112845328A true CN112845328A (en) | 2021-05-28 |
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CN202110310855.4A Pending CN112845328A (en) | 2021-03-23 | 2021-03-23 | Titanium sheet flushing device for cell biology research |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116159795A (en) * | 2023-03-14 | 2023-05-26 | 湖北东田微科技股份有限公司 | Optical lens cleaning system |
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