CN112300936A - Laboratory bench for cell culture - Google Patents
Laboratory bench for cell culture Download PDFInfo
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- CN112300936A CN112300936A CN202011231212.2A CN202011231212A CN112300936A CN 112300936 A CN112300936 A CN 112300936A CN 202011231212 A CN202011231212 A CN 202011231212A CN 112300936 A CN112300936 A CN 112300936A
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- disinfectant
- tank
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- 238000004113 cell culture Methods 0.000 title claims abstract description 60
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 83
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 53
- 239000000645 desinfectant Substances 0.000 claims abstract description 46
- 238000002474 experimental method Methods 0.000 claims abstract description 43
- 238000001802 infusion Methods 0.000 claims abstract description 31
- 230000005540 biological transmission Effects 0.000 claims abstract description 28
- 239000007921 spray Substances 0.000 claims abstract description 20
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 238000005192 partition Methods 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims description 24
- 238000010438 heat treatment Methods 0.000 claims description 22
- 238000012546 transfer Methods 0.000 claims description 18
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 13
- 238000001179 sorption measurement Methods 0.000 claims description 12
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 description 16
- 238000004140 cleaning Methods 0.000 description 7
- 238000005086 pumping Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 4
- 230000002070 germicidal effect Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000249 desinfective effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000002633 protecting effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
- F26B21/001—Drying-air generating units, e.g. movable, independent of drying enclosure
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/15—Biocide distribution means, e.g. nozzles, pumps, manifolds, fans, baffles, sprayers
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/283—Treatment of water, waste water, or sewage by sorption using coal, charred products, or inorganic mixtures containing them
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- General Chemical & Material Sciences (AREA)
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Abstract
The invention discloses a cell culture experiment table which comprises an experiment table, a partition plate, a water tank, a disinfectant tank and a second infusion pump, wherein a cell culture storage device and a placing frame are arranged at the upper end of the experiment table, the cell culture storage device is arranged on the rear side of the placing frame, a microscopic observation device is arranged on the right side of the placing frame, a supporting mechanism is arranged below the microscopic observation device, one end of a first spray pipe, far away from the first infusion pump, penetrates through the inner front side wall of the water tank and is arranged above a collecting pool, one end of a second spray pipe, far away from the second infusion pump, penetrates through the inner front side wall of a disinfection box and is arranged above the collecting pool, and first scales are arranged on the front sides of the disinfection box and the water. This laboratory bench for cell culture adds proper amount to the disinfect box with the disinfectant in the disinfectant jar in, to adding proper amount water in the disinfect box, drives the stirring leaf through pivot, transmission shaft and rotates to disinfectant and water intensive mixing stirring under the effect of motor, can be used to the disinfection use to the laboratory glassware.
Description
Technical Field
The invention relates to the technical field related to cell culture, in particular to a cell culture experiment table.
Background
The cell can take out the right amount from the incubator usually and carry out the experiment inspection at the culture process, observes the state condition of cell, because the cell is higher to aseptic requirement, all need the disinfection of disinfecting to the utensil that uses in the experiment to and the utensil after the use carries out corresponding disinfection and sterilization and handle, the test tube size that needs to use in addition in the experimentation is different, need corresponding solution to the stable storage of test tube, needs a cell culture to solve above-mentioned problem with the laboratory bench now.
Disclosure of Invention
The invention aims to provide a cell culture experiment table, which aims to solve the problems that instruments used in an experiment need to be sterilized and disinfected due to high requirements of cells on sterility, the instruments used after the instruments are used for corresponding sterilization and disinfection treatment, and in addition, test tubes used in the experiment process are different in size, and the problem that the stable storage of the test tubes needs to be correspondingly solved.
In order to achieve the purpose, the invention provides the following technical scheme: a cell culture experiment table comprises an experiment table, a clapboard, a water tank, a disinfectant tank and a second infusion pump,
the cell culture storage device is arranged at the rear side of the placing rack and comprises a culture medium, a heating element, a temperature sensor and a controller, the culture medium is arranged in the cell culture storage device, the heating element is arranged on the inner side wall of the cell culture storage device, the temperature sensor and the controller are arranged at the top end in the cell culture storage device, the temperature sensor, the controller and the heating element are electrically connected, the microscopic observation device is arranged on the right side of the placing rack, a supporting mechanism is arranged below the microscopic observation device, and the supporting mechanism and the microscopic observation device are arranged on the experiment table;
the partition plate is arranged at the upper end of the experiment table, the partition plate is arranged on the right side of the cell culture storage device, the drying box and the water pool are arranged between the partition plates, the drying box is arranged on the front side of the water pool, the water pool is arranged on the experiment table, and the water faucet is arranged on the left side of the water pool;
the device comprises a water tank, a disinfection box is arranged on the left side of the water tank, the disinfection box and the water tank are both arranged at the upper end of an experiment table, the disinfection box is arranged on the right side of a partition plate, a first infusion pump is arranged in the water tank and is connected with a first spray pipe, one end, far away from the first infusion pump, of the first spray pipe penetrates through the inner front side wall of the water tank and is arranged above a collecting pool, the collecting pool is arranged on the experiment table, and the collecting pool is arranged on the front side of the water tank;
the disinfectant tank is arranged on the disinfection box, the upper end of the disinfection box is provided with a motor, the motor is connected with a rotating shaft, the lower end of the rotating shaft penetrates through the upper end of the disinfection box and is connected with a transmission shaft, the transmission shaft is rotatably connected to the inner top end of the disinfection box, and the transmission shaft is provided with stirring blades;
the second transfer pump, the second transfer pump sets up in the disinfect box, and the second transfer pump is connected with the second spray tube, the one end that the second transfer pump was kept away from to the second spray tube runs through the inside preceding lateral wall setting of disinfect box in the top of collecting pit, and the front side of disinfect box and water tank all is provided with first scale.
Preferably, the experiment table is including holding tank, sterilamp, instrument drawer and through-hole, and is provided with the holding tank in the experiment table, the holding tank sets up in the left the place ahead in pond, and the holding tank in top and interior bottom all be provided with the sterilamp, be provided with the instrument drawer in the holding tank, and the bottom is provided with the through-hole in the instrument drawer.
Preferably, the placing frame comprises a first supporting plate, a rubber base, a second supporting plate, a placing hole and a rubber pad, the rubber base is arranged at the upper end of the first supporting plate, the upper end of the first supporting plate is connected with the second supporting plate, the placing hole is formed in the second supporting plate, and the rubber pad is arranged on the inner side wall of the placing hole.
Preferably, the second support plate is uniformly provided with the placing holes, the inner diameters of the placing holes in each group are different, and each placing hole corresponds to one rubber base.
Preferably, the supporting mechanism comprises a first slide rail, a first placing table, a second slide rail and a second placing table, the first slide rail is arranged at the upper end of the experiment table, the first placing table is arranged on the first slide rail, the second slide rail is arranged on the first placing table, the second placing table is arranged on the second slide rail, and the second placing table is perpendicular to the first placing table.
Preferably, the drying cabinet is including perforated plate, fan, heating element and connecting pipe, and is provided with the perforated plate in the drying cabinet, the drying cabinet inside wall is provided with the fan, and the back lateral wall is provided with heating element in the drying cabinet, and the drying cabinet rear side is linked together through connecting pipe and pond simultaneously.
Preferably, the disinfectant tank comprises a feed inlet, second scales and an infusion tube, the feed inlet is formed in the upper end of the disinfectant tank, the second scales are arranged on the front side of the disinfectant tank, and the infusion tube is arranged at the lower end of the disinfectant tank.
Preferably, the motor, the rotating shaft, the transmission shaft and the stirring blade form a rotating mechanism.
Preferably, the collecting pit is including disinfection liquid pond, catch basin, filter screen layer, active carbon adsorption layer, third transfer pump and transmission pipe, and is provided with disinfection liquid pond and catch basin in the collecting pit, and the disinfection liquid pond sets up in the catch basin left side simultaneously, the catch basin inside wall is provided with filter screen layer and active carbon adsorption layer, and the filter screen layer sets up in active carbon adsorption layer top, the bottom is provided with the third transfer pump in the catch basin, and the third transfer pump is connected with the transmission pipe, and the transmission pipe is kept away from third transfer pump one end simultaneously and is run through the catch basin and communicate with the disinfect box.
Compared with the prior art, the invention has the beneficial effects that: the experimental table for cell culture is provided with a plurality of experimental tables,
(1) according to the requirements of relevant experiments such as cell culture, detection and the like, a corresponding test tube can be selected to take out a proper amount of cell sap from the cell culture storage device for use, if cells need to be observed and detected, the cell sap can be dripped on the supporting mechanism, observation is carried out through a microscopic observation device, the first placing table slides on the first slide rail or the second placing table slides on the second slide rail for adjustment, and therefore the experiment personnel can conveniently and comprehensively detect and observe;
(2) under the auxiliary action of the second scale, adding a proper amount of disinfectant in the disinfectant tank into the disinfection box, adding a proper amount of water into the disinfection box, and driving the stirring blades to rotate to fully mix and stir the disinfectant and the water through the rotating shaft and the transmission shaft under the action of the motor, so that the disinfectant tank can be used for disinfecting experimental instruments;
(3) pumping disinfectant solution in a disinfection box under the action of a second liquid pumping pump, spraying the disinfectant solution out of the disinfection box through a second spray pipe to disinfect and clean the experimental apparatus, pumping water in a water tank under the action of a first liquid pumping pump, spraying the water out of the water tank through a first spray pipe to flush the surface of the experimental apparatus again, placing the apparatus after disinfection and cleaning on a porous plate, drying the apparatus through a fan and a heating element, placing the apparatus after disinfection, cleaning and drying into a tool drawer for storage, performing sterilization treatment again under the action of an ultraviolet sterilization lamp to improve the sanitary quality of the apparatus, pumping cleaning water in a water collecting tank into the disinfection box through a transmission pipe under the action of a third liquid pumping pump after being filtered through a filter screen layer and subjected to adsorption treatment by an active carbon adsorption layer, adding a proper amount of disinfectant, mixing and stirring to form disinfectant solution, and recycling the cleaning water;
(4) the first scales arranged on the disinfection box and the water tank are convenient for observing the capacities of disinfectant solution and water inside, and can be added in time, in addition, the placing rack is provided with a plurality of groups of placing holes with different inner diameter sizes for fixedly placing experimental test tubes with different sizes, the rubber pads enhance the placing stability of the test tubes, and simultaneously play a certain protection effect on the test tubes;
(5) be provided with temperature sensor in the cell culture strorage device, under temperature sensor's effect, when the temperature in the cell culture strorage device is less than the setting value, accessible controller control heating element heats in to the cell culture strorage device, keeps proper temperature in the cell culture strorage device, is fit for cell culture, avoids low temperature to influence the enzymatic activity, influences biological reaction rate to influence cell culture.
Drawings
FIG. 1 is a schematic view of a front cross-sectional structure of an experiment table according to the present invention;
FIG. 2 is a schematic structural diagram of a filter screen layer and an activated carbon adsorption layer according to the present invention;
FIG. 3 is a schematic view of an inner structure of the accommodating groove of the invention;
FIG. 4 is a schematic view of the internal structure of the sterilization case of the present invention;
FIG. 5 is a schematic top view of the experiment table of the present invention;
FIG. 6 is a schematic view of the structure of the rack of the present invention;
FIG. 7 is a schematic structural view of a support mechanism according to the present invention;
FIG. 8 is a schematic view of the cell culture and storage device according to the present invention.
In the figure: 1. laboratory table, 101, holding tank, 102, sterilamp, 103, tool drawer, 104, through hole, 2, cell culture storage device, 201, culture medium, 202, heating element, 203, temperature sensor, 204, controller, 3, rack, 301, first support plate, 302, rubber base, 303, second support plate, 304, placing hole, 305, rubber pad, 4, microscopic observation device, 5, support mechanism, 501, first slide rail, 502, first placing table, 503, second slide rail, 504, second placing table, 6, partition plate, 7, drying box, 701, perforated plate, 702, fan, 703, heating element, 704, connecting pipe, 8, water tank, 9, faucet, 10, water tank, 11, disinfection box, 12, first infusion pump, 13, first spray pipe, 14, disinfectant tank, 1401, feed inlet, 1402, second scale, 1403, 15, motor, 16. the device comprises a rotating shaft, 17, a transmission shaft, 18, a stirring blade, 19, a second infusion pump, 20, a second spray pipe, 21, first scales, 22, a collecting tank, 2201, a disinfectant tank, 2202, a water collecting tank, 2203, a filter screen layer, 2204, an active carbon adsorption layer, 2205, a third infusion pump, 2206 and a delivery pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-8, the present invention provides a technical solution: a cell culture experiment table, as shown in FIG. 1, FIG. 5, FIG. 6, FIG. 7 and FIG. 8, a cell culture storage device 2 and a rack 3 are arranged on the upper end of an experiment table 1, the cell culture storage device 2 is arranged on the rear side of the rack 3, the experiment table 1 comprises a holding tank 101, an ultraviolet germicidal lamp 102, a tool drawer 103 and a through hole 104, the holding tank 101 is arranged in the experiment table 1, the holding tank 101 is arranged on the left front side of a water tank 8, the ultraviolet germicidal lamp 102 is arranged on the top end and the bottom in the holding tank 101, the tool drawer 103 is arranged in the holding tank 101, the through hole 104 is arranged on the bottom in the tool drawer 103, under the action of the ultraviolet germicidal lamps 102 on the upper and lower sides, the instruments in the holding tank 101 can be sufficiently sterilized again, and the sanitation quality is improved, the cell culture storage device 2 comprises a culture medium 201, a heating element 202, a temperature sensor, the cell culture storage device 2 is internally provided with a culture medium 201, the inner side wall of the cell culture storage device 2 is provided with a heating element 202, the inner top end of the cell culture storage device 2 is provided with a temperature sensor 203 and a controller 204, the temperature sensor 203, the controller 204 and the heating element 202 are electrically connected, the placing rack 3 comprises a first support plate 301, a rubber base 302, a second support plate 303, placing holes 304 and a rubber pad 305, the upper end of the first support plate 301 is provided with the rubber base 302, the upper end of the first support plate 301 is connected with the second support plate 303, the second support plate 303 is provided with the placing holes 304, the inner side walls of the placing holes 304 are provided with the rubber pads 305, the placing holes 304 are provided with a plurality of groups, the placing holes 304 among each group have different inner diameters and can be used for fixing and placing test tubes with different sizes, the placing holes 304 are uniformly arranged on the second support plate 303, and the placing holes 304 among each, meanwhile, each placing hole 304 corresponds to one rubber base 302, the rubber base 302 supports and protects the bottom of a test tube, the rubber pads 305 arranged in the placing holes 304 enhance the stable placing and protecting effect on the test tube, the microscopic observation device 4 is arranged on the right side of the placing rack 3, the supporting mechanism 5 is arranged below the microscopic observation device 4, the supporting mechanism 5 and the microscopic observation device 4 are both arranged on the experiment table 1, the supporting mechanism 5 comprises a first slide rail 501, a first placing table 502, a second slide rail 503 and a second placing table 504, the first slide rail 501 is arranged at the upper end of the experiment table 1, the first placing table 502 is arranged on the first slide rail 501, the second slide rail 503 is arranged on the first placing table 502, the second placing table 504 is arranged on the second slide rail 503, and the second placing table 504 and the first placing table 502 are in a vertical relation, the first placing table 502 slides on the first slide rail 501 or the second placing table 503 slides on the second slide rail 504 for adjustment, the testing and observation of experimenters are facilitated more comprehensively.
As shown in fig. 1, 2, 3 and 5, a partition 6 is disposed at the upper end of the experiment table 1, the partition 6 is disposed at the right side of the cell culture storage device 2, a drying box 7 and a water tank 8 are disposed between the partitions 6, the drying box 7 is disposed at the front side of the water tank 8, the drying box 7 comprises a porous plate 701, a fan 702, a heating element 703 and a connecting pipe 704, a porous plate 701 is disposed in the drying box 7, a fan 702 is disposed on the inner side wall of the drying box 7, a heating element 703 is disposed on the inner rear side wall of the drying box 7, the rear side of the drying box 7 is communicated with the water tank 8 through the connecting pipe 704, the sterilized and cleaned instruments are dried under the action of the fan 702 and the heating element 703, the porous plate 701 facilitates the dripping of water stains on the instruments, the water stains are discharged into the water tank 8 through the connecting pipe 704, the water tank 8 is disposed on the, the left side of the water tank 10 is provided with a disinfection tank 11, the disinfection tank 11 and the water tank 10 are both arranged at the upper end of the experiment table 1, the disinfection tank 11 is arranged at the right side of the partition plate 6, a first infusion pump 12 is arranged in the water tank 10, the first infusion pump 12 is connected with a first spray pipe 13, one end, far away from the first infusion pump 12, of the first spray pipe 13 penetrates through the inner front side wall of the water tank 10 and is arranged above a collection pool 22, the collection pool 22 is arranged on the experiment table 1, the collection pool 22 is arranged at the front side of the water tank 10, the collection pool 22 comprises a disinfection liquid pool 2201, a water collection pool 2202, a filter screen layer 2203, an active carbon adsorption layer 2204, a third infusion pump 2205 and a transmission pipe 2206, the collection pool 22 is internally provided with the disinfection liquid pool 2201 and the water collection pool 2202, the disinfection liquid pool 2201 is arranged at the left side of the water collection pool 2202, the inner side wall of the collection pool 2202 is provided with the filter screen layer, the bottom in the water collecting pool 2202 is provided with a third infusion pump 2205, the third infusion pump 2205 is connected with a transmission pipe 2206, one end of the transmission pipe 2206, which is far away from the third infusion pump 2205, penetrates through the water collecting pool 2202 and is communicated with the disinfection box 11, the washing water is filtered and adsorbed under the action of the filter screen layer 2203 and the activated carbon adsorption layer 2204, and the washing water can be pumped into the disinfection box 11 through the third infusion pump 2205 to be used for mixing and preparing disinfectant solution.
As shown in fig. 1 and 4, a disinfectant tank 14 is disposed on a disinfection tank 11, a motor 15 is disposed at the upper end of the disinfection tank 11, the disinfectant tank 14 includes a feed port 1401, a second scale 1402 and an infusion tube 1403, the feed port 1401 is disposed at the upper end of the disinfectant tank 14, the second scale 1402 is disposed at the front side of the disinfectant tank 14, the infusion tube 1403 is disposed at the lower end of the disinfectant tank 14, under the auxiliary action of the second scale 1402, the disinfectant in the disinfectant tank 14 can be conveniently controlled to be added into the disinfection tank 11, the motor 15, a rotating shaft 16, a transmission shaft 17 and a stirring blade 18 constitute a rotating mechanism, the motor 15 drives the stirring blade 18 to rotate through the rotating shaft 6 and the transmission shaft 17 to sufficiently stir the disinfectant and the water in the disinfection tank 11, the motor 15 is connected with the rotating shaft 16, the lower end of the rotating shaft 16 penetrates through the upper end of the disinfection tank 11 to be connected with the transmission shaft, and the transmission shaft 17 is provided with a stirring blade 18, the second infusion pump 19 is arranged in the disinfection box 11, the second infusion pump 19 is connected with the second spray pipe 20, one end of the second spray pipe 20, which is far away from the second infusion pump 19, penetrates through the inner front side wall of the disinfection box 11 and is arranged above the collection pool 22, and the front sides of the disinfection box 11 and the water tank 10 are both provided with first scales 21.
The working principle is as follows: when the cell culture storage device 2 is used, the power supply is switched on, when cell culture detection is carried out, a proper amount of cell culture storage device 2 can be taken out through the test tube for carrying out corresponding cell experiments and detection, when cells need to be observed and detected, cell sap can be dripped on the supporting mechanism 5, observation is carried out through the microscopic observation device 4, the first placing platform 502 slides on the first slide rail 501 or the second placing platform 504 slides on the second slide rail 503 for adjustment, more comprehensive detection and observation of experimenters are facilitated, placing holes 304 with different inner diameter sizes are arranged on the placing rack 3 and used for fixedly placing test tubes with different sizes, the rubber pad 305 enhances the placing stability of the test tube and simultaneously plays a certain protection effect on the test tube, under the action of the temperature sensor 203, when the temperature in the cell culture storage device 2 is lower than a set value, the heating element 202 can be controlled by the controller 204 to heat the cell culture storage device 2, keeping a proper temperature in the cell culture storage device 2, being suitable for cell culture, avoiding low temperature from influencing enzyme activity and biological reaction rate, and further influencing cell culture, wherein the model of the temperature sensor 203 is CWDZ11, the experimental apparatus needs to be disinfected and cleaned after being used, the transfusion tube 1403 is opened, under the auxiliary action of the second scale 1402, the disinfectant in the disinfectant tank 14 is firstly added into a proper amount in the disinfection box 11, a proper amount of water is added into the disinfection box 11, the motor 15 drives the rotating shaft 16 to rotate, the rotating shaft 16 drives the transmission shaft 17 to rotate, the transmission shaft 17 drives the stirring blade 18 to mix and stir the disinfectant and the water, under the action of the second infusion pump 19, the disinfectant solution is sprayed out through the second spray tube 20 to disinfect and clean the apparatus, under the action of the first infusion pump 12, the water in the water tank 10 is sprayed out through the first spray tube 13 to wash the apparatus after disinfection and cleaning is placed on the porous, the water stain on the utensil is convenient to drip through the porous plate 701 after being dried by the fan 702 and the heating element 703, the water stain is drained into the water tank 8 through the connecting pipe 704, the disinfected, cleaned and dried utensil can be placed into the tool drawer 103 for storage, and is sterilized again under the action of the ultraviolet germicidal lamp 102, the sanitary quality of the utensil is improved, in addition, cleaning water in the water collecting tank 2202 can be filtered through the filter screen layer 2203 and is absorbed by the active carbon absorption layer 2204, and then is pumped into the disinfection box 11 through the transmission pipe 2206 under the action of the third liquid pumping pump 2205, and then a proper amount of disinfectant is added to be mixed and stirred into a disinfectant solution, and the cleaning water is reused.
The terms "central," "longitudinal," "lateral," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for simplicity of description only and are not intended to indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operative in a particular orientation, and are not to be considered limiting of the claimed invention.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (9)
1. The utility model provides a laboratory bench for cell culture, includes laboratory table (1), baffle (6), water tank (10), disinfectant jar (14) and second transfer pump (19), its characterized in that:
an experiment table (1), wherein a cell culture storage device (2) and a placing rack (3) are arranged at the upper end of the experiment table (1), the cell culture storage device (2) is arranged at the rear side of the placing rack (3), the cell culture storage device (2) comprises a culture medium (201), a heating element (202), a temperature sensor (203) and a controller (204), the culture medium (201) is arranged in the cell culture storage device (2), the heating element (202) is arranged on the inner side wall of the cell culture storage device (2), the temperature sensor (203) and the controller (204) are arranged at the top end in the cell culture storage device (2), meanwhile, the temperature sensor (203), the controller (204) and the heating element (202) are electrically connected, a microscopic observation device (4) is arranged at the right side of the placing rack (3), and a supporting mechanism (5) is arranged below the microscopic observation device (4), meanwhile, the supporting mechanism (5) and the microscopic observation device (4) are both arranged on the experiment table (1);
the cell culture storage device comprises partition plates (6), wherein the partition plates (6) are arranged at the upper end of an experiment table (1), the partition plates (6) are arranged on the right side of the cell culture storage device (2), drying boxes (7) and a water pool (8) are arranged between the partition plates (6), the drying boxes (7) are arranged on the front side of the water pool (8), the water pool (8) is arranged on the experiment table (1), and a water faucet (9) is arranged on the left side of the water pool (8); the water tank (10), the left side of water tank (10) is provided with disinfect box (11), and disinfect box (11) and water tank (10) all set up in experiment table (1) upper end, and disinfect box (11) set up the right side at baffle (6) simultaneously, be provided with first transfer pump (12) in water tank (10), and first transfer pump (12) are connected with first spray tube (13), the one end that first transfer pump (12) was kept away from in first spray tube (13) runs through the interior preceding lateral wall setting of water tank (10) above collecting pit (22), and collecting pit (22) set up on experiment table (1), and collecting pit (22) set up in water tank (10) front side simultaneously;
the disinfectant tank (14) is arranged on the disinfectant box (11), the upper end of the disinfectant box (11) is provided with a motor (15), the motor (15) is connected with a rotating shaft (16), the lower end of the rotating shaft (16) penetrates through the upper end of the disinfectant box (11) and is connected with a transmission shaft (17), the transmission shaft (17) is rotatably connected to the inner top end of the disinfectant box (11), and the transmission shaft (17) is provided with a stirring blade (18); the second transfer pump (19), second transfer pump (19) set up in disinfect box (11), and second transfer pump (19) are connected with second spray tube (20), the one end that second transfer pump (19) were kept away from in second spray tube (20) runs through in disinfect box (11) preceding lateral wall setting in the top of collecting pit (22), and the front side of disinfect box (11) and water tank (10) all is provided with first scale (21).
2. A cell culture laboratory table according to claim 1, wherein: experiment table (1) is including holding tank (101), sterilamp (102), instrument drawer (103) and through-hole (104), and is provided with holding tank (101) in experiment table (1), holding tank (101) set up the left place ahead in pond (8), and the top all is provided with sterilamp (102) in holding tank (101) and the interior bottom, be provided with instrument drawer (103) in holding tank (101), and the bottom is provided with through-hole (104) in instrument drawer (103).
3. A cell culture laboratory table according to claim 1, wherein: the placing frame (3) comprises a first support plate (301), a rubber base (302), a second support plate (303), placing holes (304) and rubber pads (305), the rubber base (302) is arranged at the upper end of the first support plate (301), the upper end of the first support plate (301) is connected with the second support plate (303), placing holes (304) are formed in the second support plate (303), and the rubber pads (305) are arranged on the inner side walls of the placing holes (304).
4. A cell culture laboratory table according to claim 3, wherein: the second support plate (303) is uniformly provided with placing holes (304), the inner diameters of the placing holes (304) in each group are different, and each placing hole (304) corresponds to one rubber base (302).
5. A cell culture laboratory table according to claim 1, wherein: the supporting mechanism (5) comprises a first sliding rail (501), a first placing table (502), a second sliding rail (503) and a second placing table (504), the first sliding rail (501) is arranged at the upper end of the experiment table (1), the first placing table (502) is arranged on the first sliding rail (501), the second sliding rail (503) is arranged on the first placing table (502), the second placing table (504) is arranged on the second sliding rail (503), and the second placing table (504) and the first placing table (502) are in a vertical relation.
6. A cell culture laboratory table according to claim 1, wherein: drying cabinet (7) is including perforated plate (701), fan (702), heating element (703) and connecting pipe (704), and is provided with perforated plate (701) in drying cabinet (7), drying cabinet (7) inside wall is provided with fan (702), and the rear side wall is provided with heating element (703) in drying cabinet (7), and drying cabinet (7) rear side is linked together through connecting pipe (704) and pond (8) simultaneously.
7. A cell culture laboratory table according to claim 1, wherein: disinfectant jar (14) is including charge door (1401), second scale (1402) and transfer line (1403), and disinfectant jar (14) upper end is provided with charge door (1401), disinfectant jar (14) front side is provided with second scale (1402), and disinfectant jar (14) lower extreme is provided with transfer line (1403).
8. A cell culture laboratory table according to claim 1, wherein: the motor (15), the rotating shaft (16), the transmission shaft (17) and the stirring blade (18) form a rotating mechanism.
9. A cell culture laboratory table according to claim 1, wherein: the collecting pool (22) comprises a disinfection liquid pool (2201), a water collecting pool (2202), a filter screen layer (2203), an active carbon adsorption layer (2204), a third infusion pump (2205) and a transmission pipe (2206), the disinfection liquid pool (2201) and the water collecting pool (2202) are arranged in the collecting pool (22), the disinfection liquid pool (2201) is arranged on the left side of the water collecting pool (2202), the filter screen layer (2203) and the active carbon adsorption layer (2204) are arranged on the inner side wall of the water collecting pool (2202), the filter screen layer (2203) is arranged above the active carbon adsorption layer (2204), the third infusion pump (2205) is arranged at the inner bottom of the water collecting pool (2202), the third infusion pump (2205) is connected with the transmission pipe (2206), and one end, far away from the third infusion pump (2205), of the transmission pipe (2206) penetrates through the water collecting pool (2202) and is communicated with the disinfection infusion tank (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011231212.2A CN112300936A (en) | 2020-12-15 | 2020-12-15 | Laboratory bench for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011231212.2A CN112300936A (en) | 2020-12-15 | 2020-12-15 | Laboratory bench for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112300936A true CN112300936A (en) | 2021-02-02 |
Family
ID=74325184
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011231212.2A Withdrawn CN112300936A (en) | 2020-12-15 | 2020-12-15 | Laboratory bench for cell culture |
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| Country | Link |
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| CN (1) | CN112300936A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113145198A (en) * | 2021-04-06 | 2021-07-23 | 贵州医科大学 | Experiment operation platform for molecular biology experiment |
| CN114276926A (en) * | 2021-12-08 | 2022-04-05 | 海南浙江大学研究院 | Hu sheep embryo in vitro incubator with protection casing |
-
2020
- 2020-12-15 CN CN202011231212.2A patent/CN112300936A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113145198A (en) * | 2021-04-06 | 2021-07-23 | 贵州医科大学 | Experiment operation platform for molecular biology experiment |
| CN114276926A (en) * | 2021-12-08 | 2022-04-05 | 海南浙江大学研究院 | Hu sheep embryo in vitro incubator with protection casing |
| CN114276926B (en) * | 2021-12-08 | 2023-11-03 | 浙江大学海南研究院 | Hu sheep embryo in vitro incubator with protection casing |
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Application publication date: 20210202 |
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