CN112826766A - Ganoderma mouth wash and preparation method thereof - Google Patents
Ganoderma mouth wash and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/86—Polyethers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/30—Characterized by the absence of a particular group of ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the technical field of daily chemical articles, and particularly relates to a ganoderma lucidum mouthwash and a preparation method thereof. The feed comprises the following raw materials in parts by weight: 100-200 parts of ganoderma lucidum extract, 35-45 parts of erythritol, 0.1-0.2 part of stevioside, 4070.4-0.6 part of poloxamer, 5-10 parts of fresh flower absolute and 745-860 parts of purified water. The ganoderma lucidum mouthwash does not contain ethanol or chemical synthetic drugs, is high in safety, can avoid side effects caused by mistaken taking, and is suitable for long-term use; can inhibit the proliferation of harmful bacteria in oral cavity and regulate the immune environment of oral cavity, and can assist in repairing recurrent oral ulcer; can avoid tongue fur and tooth staining, has high effective active ingredient, and triterpene and sterol concentration of not less than 1mg/ml, can maintain oral cavity environment pH neutral, and inhibit tooth decalcification and dental plaque formation; fresh breath, cool mouthfeel and no residual medicinal flavor; the preparation method is simple, and the production process is environment-friendly and pollution-free.
Description
Technical Field
The invention belongs to the technical field of daily chemical articles, and particularly relates to a ganoderma lucidum mouthwash and a preparation method thereof.
Background
The conventional tooth brushing can only remove 40 to 50 percent of dental plaque and 30 percent of adjacent dental plaque, and has no effect on removing the dental plaque at the gingival margin, between the teeth and on the surface of the soft tissue of the oral cavity. The gargle can be deep in various parts of the oral cavity of a person, including the gingival edge and the slits between teeth which are difficult to contact with the toothbrush, and is a good measure for assisting in controlling dental plaque. The mouthwash becomes an indispensable oral cleaning product in modern life, has the advantage of convenient use compared with toothpaste, and is the best choice for guaranteeing oral hygiene for old people with teeth falling off and patients with odontopathy who cannot tolerate tooth brushing. In recent years, with the increasing health consciousness of people, the requirement for the mouthwash is higher and higher, and the auxiliary prevention of oral diseases besides the tooth cleaning is also needed.
However, most of the traditional mouth wash is added with alcohol components to play a role in cleaning and sterilizing, but the traditional mouth wash has strong stimulation and is difficult to accept by people. A great deal of research has shown that alcohol has many adverse effects such as irritation to mucous membrane, aggravation of oral ulcer, carcinogenesis, toxicity and influence on the structure of the appliance.
At present, chemical antibacterial agents such as cetylpyridinium chloride, chlorhexidine, benzalkonium chloride, triclosan and the like are mostly used in the alcohol-free mouth wash, and the chemical antibacterial agents have local side effects and are easy to generate hidden troubles such as drug resistance, tooth staining or dry mouth after long-term use. In the prior art, a plurality of mouthwash schemes with natural product extracts are added, but because of the reasons of low and unstable active ingredients or low bacteriostatic activity of bacteriostatic ingredients, the bacteriostatic effect is not ideal, the stability is poor, and most of the mouthwash is compounded with chemical antibacterial agents and chemical preservatives.
In recent years, with the development of the modern technology of natural product extracts, the method for researching and utilizing the active ingredients of the natural product extracts is well developed, and the method provides good technical support for assisting in preventing oral diseases by utilizing the active ingredients of the natural product extracts in the mouth wash. However, the natural product extract is brown or black, bitter and astringent in taste and has a very strong traditional Chinese medicine taste, so that a certain traditional Chinese medicine taste is still remained in the oral cavity after the natural product extract is used as the mouth wash, and the tongue fur and the teeth are dyed by the color of the extract, so that the natural product extract is resistant to a user and cannot be popularized and applied, and can only be used as an auxiliary product for oral treatment.
Meanwhile, in terms of safety, some mouthwashes are used without long-term eating of traditional natural products such as antrodia camphorata and the like, and have certain potential toxicity; the sweetener such as sorbitol and xylitol is used, and although not causing dental caries, the sweetener can cause diarrhea and digestive disorder if being swallowed excessively by mistake; the flavoring agent such as menthol or chemical synthetic perfume has potential irritation and anaphylaxis.
Disclosure of Invention
The invention aims to overcome the defects of the existing mouthwash, and provides the ganoderma lucidum mouthwash which does not contain alcohol components, does not contain chemical antibacterial agents, is extracted from natural products, has no toxicity, can inhibit the proliferation of harmful bacteria in the oral cavity, adjust the immune environment of the oral cavity, can assist or replace to repair recurrent oral ulcer, can avoid tongue fur and tooth staining, is safe and reliable, and is suitable for long-term use, and the preparation method thereof.
The technical scheme adopted by the invention is as follows: a ganoderma mouth wash is characterized in that: the feed comprises the following raw materials in parts by weight: 100-200 parts of ganoderma lucidum extract, 35-45 parts of erythritol, 0.1-0.2 part of stevioside, 4070.4-0.6 part of poloxamer, 5-10 parts of fresh flower absolute and 845 parts of purified water 760-containing material.
The invention creatively applies the ganoderma lucidum into the mouthwash, and utilizes the active ingredients of the ganoderma lucidum to inhibit the proliferation of harmful bacteria in the oral cavity and regulate the immune environment of the oral cavity.
Ganoderma (Ganoderma lucidum (Leys. ex Fr.) Karst.) is one of Ganoderma (China pharmacopoeia 2020 edition) of Polyporaceae, has sweet taste and mild property, and has main active ingredients of Ganoderma triterpene and ganoderan, and Ganoderma triterpene has effects of regulating immunity, resisting inflammation, resisting allergy and inhibiting bacteria.
With the development of modern natural product separation and detection technology, the efficacy of active ingredients of ganoderma lucidum serving as a long-history, safe and effective traditional Chinese medicine is verified in many ways. The ganoderma triterpene, one of the active components in ganoderma, has been reported to have significant bacteriostatic action (Wangwangwu et al, "extraction and separation of ganoderma acid component and bacteriostatic activity research [ J ]", university of Beijing university of science and technology, 2002 (22): 125-. Meanwhile, the Ganoderma lucidum also has the efficacy of regulating the immune environment of the oral cavity of a human body, and can assist or alternatively repair recurrent oral ulcer (Liangguojia, etc., clinical observation and discussion of Ganoderma lucidum capsule for treating recurrent aphthous ulcer, Chinese medical guidance, 2011, 9 (14): 295-296; Xie L, et al.
The proportion of erythritol and stevioside in the final ganoderma lucidum mouthwash and the proportion of erythritol and stevioside have obvious influence on the taste masking effect of the ganoderma lucidum extract in the ganoderma lucidum mouthwash, and through a large number of experiments and analyses, 35-45 parts of erythritol and 0.1-0.2 part of stevioside have optimal taste masking effect on the bitterness of the ganoderma lucidum extract and the sensory taste effect of the final ganoderma lucidum mouthwash.
The fresh flower absolute oil has obvious influence on the taste masking effect of the lucid ganoderma extract liquid in the lucid ganoderma mouthwash in the final proportion of the lucid ganoderma mouthwash and the proportion of the fresh flower absolute oil and the poloxamer 407, and through a large number of experiments and analyses, 5-10 parts of the fresh flower absolute oil and 4070.4-0.6 part of the poloxamer have the optimal taste masking effect on the traditional Chinese medicine taste of the lucid ganoderma extract liquid and the optimal sensory and sensory effects of the lucid ganoderma mouthwash.
Since Ganoderma lucidum contains a large amount of melanin, part of the melanin and its derivatives are soluble in ethanol and insoluble in water, and easily adhere to solid surface (CN201910799760.2, a method for extracting and separating pigment from Ganoderma sinensis waste), the color of conventional Ganoderma lucidum extract can stain tongue fur and teeth, and can not be used in mouth wash. The invention ensures the effective content of the ganoderma lucidum mouthwash by the following creative preparation method of the ganoderma lucidum extracting solution, and overcomes the defect that ganoderma lucidum can stain tongue fur and teeth.
The preparation method of the ganoderma lucidum extracting solution comprises the following steps:
1. crushing dried lucid ganoderma sporocarp with the water content of less than or equal to 6%, sieving with a 20-mesh sieve to obtain lucid ganoderma particles, adding 75% ethanol with the mass 10 times that of the lucid ganoderma particles into the lucid ganoderma particles, extracting for 2 hours at 80 ℃, filtering with a 200-mesh sieve to obtain filtrate, adding 75% ethanol with the mass 10 times that of the lucid ganoderma particles into filter residues again, extracting for 2 hours at 80 ℃, filtering with a 200-mesh sieve, combining the two extraction filtrates, concentrating, drying, crushing, and sieving with a 100-mesh sieve to obtain lucid ganoderma extract coarse powder;
2. adding 95% ethanol 8 times the weight of Ganoderma extract coarse powder into Ganoderma extract coarse powder, dissolving, adding 10% dipotassium hydrogen phosphate aqueous solution 20 times the weight of Ganoderma extract coarse powder, extracting under shaking for 10min, standing for 2 hr, and collecting the lower phase to obtain Ganoderma extract.
In the preparation method of the lucid ganoderma extract, the content of effective components in the obtained lucid ganoderma extract coarse powder is high, the quality is good, and the effects of inhibiting bacteria, cleaning and regulating the oral immune environment of the final product mouthwash can be achieved through scientific design of ethanol concentration, material-liquid ratio, extraction time and extraction times in the step 1.
Meanwhile, in the step 2 of the preparation method of the lucid ganoderma extract, coarse powder of the lucid ganoderma extract is dissolved by ethanol so as to enable melanin and the like in the coarse powder of the lucid ganoderma extract to be dissolved out again, then dipotassium hydrogen phosphate solution is used for carrying out two-aqueous phase extraction, the lucid ganoderma extract obtained by purification is used as a raw material, water-insoluble melanin components and most traditional Chinese medicine flavor components in the lucid ganoderma alcohol extract are removed, tongue fur and tooth staining can be avoided, and meanwhile residual traditional Chinese medicine flavor is light. Since the aqueous solution of dipotassium hydrogen phosphate is weakly alkaline: free potassium ions and the ganoderma triterpenic acid which is insoluble in water are combined into a salt, so that the solubility of the ganoderma triterpenes in the ganoderma extract is greatly improved, and the antibacterial activity of the ganoderma mouthwash is effectively improved; the phosphate is a pH buffer factor naturally existing in the oral cavity of a human body, and the pH of the ganoderma lucidum mouthwash is higher than the oral environment of the human body due to the buffer effect of the ganoderma lucidum extract and the dipotassium hydrogen phosphate supplemented by the ganoderma lucidum mouthwash, so that the acidic environment generated by fermenting food residues by harmful bacteria in the oral cavity is effectively inhibited, the pH neutrality of the oral environment is maintained, and the decalcification of teeth and the formation of dental plaque are inhibited.
The Ganoderma lucidum fruiting body is a dry fruiting body of Ganoderma lucidum ganodermatam lucidum (Leys. ex Fr.) Karst in Polyporaceae recorded in the 2020 edition of pharmacopoeia of the people's republic of China, and the content of triterpenes and sterols is not less than 0.50% in terms of oleanolic acid.
The method for detecting the ganoderma triterpene in the ganoderma mouthwash is a method for detecting the triterpene and the sterol under the ganoderma lucidum item recorded in the 2020 edition of pharmacopoeia of the people's republic of China, and the concentration of the triterpene and the sterol in the ganoderma mouthwash is not less than 1mg/ml calculated by oleanolic acid.
The preparation method of the flower absolute oil comprises the following steps: in the preparation method of the medium volatile absolute oil in the method for extracting and separating components of the osmanthus fragrans or the jasmine flower (CN201310684930.9), raw materials include but are not limited to the osmanthus fragrans, the jasmine flower or the rose, and the like, and the osmanthus fragrans and the jasmine flower are preferred.
Furthermore, the flower absolute oil is a mixture of one or more flower absolute oils.
The fresh flower absolute oil is the fresh flower absolute oil of the osmanthus fragrans or the jasmine or the mixture of the two fresh flower absolute oils.
The mixing ratio of the flower absolute oil of the sweet osmanthus flower or the jasmine flower is 1-3: 3-1.
The preparation method of the ganoderma lucidum mouthwash provided by the invention comprises the following steps:
1. weighing fresh flower absolute oil and poloxamer 407 according to the formula proportion, and stirring to form uniform oily solution S1;
2. weighing erythritol, stevioside and lucid ganoderma extract according to the proportion of the formula, and stirring to form a uniform aqueous solution S2;
3. slowly dropwise adding the S1 into the S2 under the stirring state, uniformly mixing, adding purified water, and uniformly mixing and stirring;
4. stopping stirring, standing at 4 deg.C for 24 hr, filtering with 0.22 μm organic membrane for sterilization, and packaging to obtain Ganoderma collutory.
According to the preparation method of the ganoderma lucidum mouthwash, the steps 3 and 4 have obvious influence on the sensory taste effect and stability of the ganoderma lucidum mouthwash, and through a large number of experiments and analyses, S1 is slowly dripped into S2 in a stirring state, the mixture is uniformly mixed, purified water is added, and the preparation method of uniformly mixing and stirring is optimal for the sensory taste effect and stability of the final ganoderma lucidum mouthwash.
Compared with the prior art, the invention has the advantages and beneficial effects that:
1. the ganoderma lucidum mouthwash does not contain ethanol or chemically synthesized medicinal components: no potential adverse effects such as mucous membrane stimulation, aggravation of oral ulcer, carcinogenicity, toxicity, influence on the structure of the appliance, drug resistance, tooth coloring or dry mouth and the like exist, and the ganoderma triterpene, the flower absolute oil, the erythritol and the stevioside disclosed by the invention have high safety: ganoderma lucidum is listed as a test point for both medicine and food at present, sweet osmanthus flower, jasmine flower, rose flower and the like are traditional edible spice and essence raw materials, and erythritol and stevioside are listed as food additives; the raw materials are swallowed by mistake in daily use, so that adverse effects on human bodies are not caused, and meanwhile, the raw materials cannot be utilized by microorganisms in oral cavities and digestive tracts, so that the oral liquid is safe and reliable and is suitable for long-term use.
2. The main active component of the ganoderma lucidum mouthwash is ganoderma lucidum triterpene, and the ganoderma lucidum mouthwash has an obvious antibacterial effect: the antibacterial agent has a good inhibiting effect on streptococcus mutans, lactobacillus acidophilus and porphyromonas gingivalis, has no adverse effects of other natural products, such as low and unstable active ingredient content, low antibacterial activity of antibacterial ingredients or unsatisfactory antibacterial effect, does not need to additionally add a preservative, and is natural, organic and environment-friendly. Meanwhile, the lucid ganoderma mouthwash can effectively relieve local inflammatory reaction, promote tissue repair and effectively shorten the healing time of dental ulcer.
3. The ganoderma mouth wash disclosed by the invention is prepared by taking a ganoderma extract obtained by extracting and purifying with ethanol and a dipotassium hydrogen phosphate solution as a raw material, removing a melanin component insoluble in water and most traditional Chinese medicine flavor components in a ganoderma alcohol extract, avoiding tongue fur and tooth staining, and simultaneously leaving a relatively light traditional Chinese medicine flavor. Since the aqueous solution of dipotassium hydrogen phosphate is weakly alkaline: free potassium ions and ganoderma triterpenic acid which is insoluble in water are combined into salt, so that the solubility of ganoderma triterpenes in ganoderma extract is greatly improved, the concentration of triterpenes and sterols in ganoderma mouthwash is not less than 1mg/ml calculated by oleanolic acid, and the antibacterial activity of ganoderma mouthwash is effectively improved; the phosphate is a pH buffer factor naturally existing in the oral cavity of a human body, and the pH of the ganoderma lucidum mouthwash is higher than the oral environment of the human body due to the buffer effect of the ganoderma lucidum extract and the dipotassium hydrogen phosphate supplemented by the ganoderma lucidum mouthwash, so that the acidic environment generated by fermenting food residues by harmful bacteria in the oral cavity is effectively inhibited, the pH neutrality of the oral environment is maintained, and the decalcification of teeth and the formation of dental plaque are inhibited.
4. The lucid ganoderma mouth wash disclosed by the invention can obviously inhibit the traditional Chinese medicine flavor of lucid ganoderma extract by using fresh flower absolute oil: fresh flowers have obvious clean oil fragrance, long duration and fresh breath; the use of erythritol and stevioside can obviously inhibit the bitterness of the ganoderma lucidum extract: the erythritol has a cool feeling, so that the mouth wash is cool and comfortable in taste, does not contain irritants, and has no residual medicinal odor in nasal cavities and oral cavities after the mouth wash is spitted out.
5. The preparation method of the ganoderma mouth wash is simple, and the production process is environment-friendly and pollution-free.
Drawings
FIG. 1 is a graph showing the experimental results of the effect of Ganoderma lucidum mouthwash on the healing time of canker sore
Detailed Description
The present invention will be described in detail below with reference to examples and drawings to facilitate understanding of the present invention by those skilled in the art. It should be particularly noted that the examples are given solely for the purpose of illustration and are not to be construed as limitations on the scope of the invention, as those skilled in the art will be able to make insubstantial modifications and variations of the invention in light of the above teachings.
A ganoderma mouth wash comprises the following raw materials in parts by weight: 100-200 parts of ganoderma lucidum extract, 35-45 parts of erythritol, 0.1-0.2 part of stevioside, 4070.4-0.6 part of poloxamer, 5-10 parts of fresh flower absolute and 745-860 parts of purified water. The concentration of triterpene and sterol in Ganoderma collutory is not less than 1mg/ml calculated by oleanolic acid. The raw materials include, but are not limited to, osmanthus fragrans, jasmine or rose, and the like, preferably the flower absolute oil of osmanthus fragrans and jasmine, or the mixture of the two flower absolute oils. The mixing ratio of the flower absolute oil of the sweet osmanthus flower or the jasmine flower is 1-3: 3-1.
The preparation method of the ganoderma lucidum extract comprises the following steps: crushing dried lucid ganoderma sporocarp with the water content of less than or equal to 6%, sieving with a 20-mesh sieve to obtain lucid ganoderma particles, adding 75% ethanol with the mass 10 times that of the lucid ganoderma particles into the lucid ganoderma particles, extracting for 2 hours at 80 ℃, filtering with a 200-mesh sieve to obtain filtrate, adding 75% ethanol with the mass 10 times that of the lucid ganoderma particles into filter residues again, extracting for 2 hours at 80 ℃, filtering with a 200-mesh sieve, combining the two extraction filtrates, concentrating, drying, crushing, and sieving with a 100-mesh sieve to obtain lucid ganoderma extract coarse powder; adding 95% ethanol 8 times the weight of Ganoderma extract coarse powder into Ganoderma extract coarse powder, dissolving, adding 10% dipotassium hydrogen phosphate aqueous solution 20 times the weight of Ganoderma extract coarse powder, extracting under shaking for 10min, standing for 2 hr, and collecting the lower phase to obtain Ganoderma extract.
A preparation method of ganoderma mouth wash comprises the following steps: 1. weighing fresh flower absolute oil and poloxamer 407 according to the formula proportion, and stirring to form uniform oily solution S1;
2. weighing erythritol, stevioside and lucid ganoderma extract according to the proportion of the formula, and stirring to form a uniform aqueous solution S2;
3. slowly dropwise adding the S1 into the S2 under the stirring state, uniformly mixing, adding purified water, and uniformly mixing and stirring;
4. stopping stirring, standing at 4 deg.C for 24 hr, filtering with 0.22 μm organic membrane for sterilization, and packaging to obtain Ganoderma collutory.
Example 1
A ganoderma mouth wash comprises the following raw materials in parts by weight: 100-200 parts of ganoderma lucidum extract, 35-45 parts of erythritol, 0.1-0.2 part of stevioside, 4070.4-0.6 part of poloxamer, 5-10 parts of fresh flower absolute and 745-860 parts of purified water.
Wherein: the preparation method of the ganoderma lucidum extract comprises the following steps:
1. crushing dried (the water content is less than or equal to 6%) ganoderma lucidum fruit bodies, sieving the crushed dried (the water content is less than or equal to 6%) ganoderma lucidum fruit bodies by a sieve of 20 meshes to obtain 10kg ganoderma lucidum particles, adding 100kg 75% ethanol into the ganoderma lucidum particles, extracting the mixture for 2 hours at 80 ℃, filtering the mixture by a filter screen of 200 meshes to obtain filtrate, adding 100kg 75% ethanol into filter residues again, extracting the mixture for 2 hours at 80 ℃, filtering the mixture by a filter screen of 200 meshes, combining the two extracted filtrates, concentrating, drying, crushing, and sieving the filtrate by a sieve of 100 meshes;
2. dissolving 500g Ganoderma extract coarse powder in 4kg 95% ethanol, adding 10kg 10% dipotassium hydrogen phosphate aqueous solution, extracting under shaking for 10min, standing for 2 hr, and collecting the lower phase to obtain about 5kg Ganoderma extract. A preparation method of ganoderma mouth wash comprises the following steps:
1. weighing 100g of sweet osmanthus flower absolute oil and 4076 g of poloxamer, and stirring to form uniform oily solution S1; 2. weighing 450g of erythritol, 2g of stevioside and 2000g of lucid ganoderma extract, and stirring to form a uniform aqueous solution S2;
3. slowly dripping S1 into S2 at a stirring speed of 300r/min under the stirring state, uniformly mixing, adding about 7450g of purified water, uniformly mixing and stirring at a stirring speed of 300 r/min;
4. stopping stirring, standing at 4 deg.C for 24 hr, filtering with 0.22 μm organic membrane for sterilization, and packaging with 20ml soft-packaged aluminum foil bag to obtain Ganoderma collutory.
Example 2
The preparation method of the ganoderma lucidum extract is the same as that of example 1.
A preparation method of ganoderma mouth wash comprises the following steps:
1. weighing 75g of jasmine flower absolute oil and 4075 g of poloxamer, and stirring to form a uniform oily solution S1; 2. weighing 400g of erythritol, 1.5g of stevioside and 1500g of the ganoderma lucidum extract in the example 1, and stirring to form a uniform aqueous solution S2;
3. slowly dripping S1 into S2 at a stirring speed of 200r/min under the stirring state, uniformly mixing at a speed of 1.3g/min, adding about 8000g of purified water, uniformly mixing and stirring at a stirring speed of 300 r/min;
4. stopping stirring, standing at 4 deg.C for 24 hr, filtering with 0.22 μm organic membrane for sterilization, and packaging with 20ml soft-packaged aluminum foil bag to obtain Ganoderma collutory.
Example 3
The preparation method of the ganoderma lucidum extract is the same as that of example 1.
A preparation method of ganoderma mouth wash comprises the following steps:
1. weighing sweet osmanthus: 3, jasmine flower: 1, stirring 50g of mixed fresh flower absolute oil and 4074 g of poloxamer to form uniform oily solution S1;
2. weighing 350g of erythritol, 1.0g of stevioside and 1000g of the lucid ganoderma extract in the embodiment 1, and stirring to form a uniform aqueous solution S2;
3. under the stirring state, the stirring speed is 150r/min, S1 is slowly dripped into S2 at the speed of 1g/min, the mixture is uniformly mixed, 8600g of purified water is added, the mixture is uniformly mixed and stirred, and the stirring speed is 300 r/min; 4. stopping stirring, standing at 4 deg.C for 24 hr, filtering with 0.22 μm organic membrane for sterilization, and packaging with 20ml soft-packaged aluminum foil bag to obtain Ganoderma collutory.
The ganoderma mouth rinses of examples 1, 2 and 3 above were tested:
1. detection of concentration of ganoderma triterpene in ganoderma mouth wash
The ganoderma mouth rinses of examples 1, 2 and 3 were tested using the method for testing triterpenes and sterols from ganoderma lucidum as recorded in the 2020 edition of the pharmacopoeia of the people's republic of China, and the test results are shown in Table 1
TABLE 1 triterpene and sterol content of Ganoderma mouth rinses
2. Antiseptic challenge for ganoderma lucidum mouthwash
Taking the experimental groups of examples 1, 2 and 3 and the control group of 0.5 percent p-hydroxyacetophenone, respectively adding mixed bacterial (staphylococcus aureus liquid, salmonella liquid and escherichia coli liquid in logarithmic phase are mixed according to the volume ratio of 1: 1: 1) and fungal (candida albicans) bacterial suspensions to make the final bacterial content of each tested sample be 1-5 × 106cfu/g bacteria and 1-5X 105cfu/g fungi. Then, the mixture was mixed well and cultured at 28 ℃. The total bacterial count and total fungal count were determined on days 0, 7, 14 and 28 of inoculation according to the experimental methods recommended by the cosmetics and fragrance flavor association (CTFA) to determine the preservative efficacy of the ganoderma mouth rinse. The judgment standard is as follows: when each sample was inoculated once, the amount of viable bacteria was reduced to not more than 0.1% of the initial concentration on day 14, and then gradually decreased, and aseptically grown on day 28. The test is passed; otherwise the test is not passed.
TABLE 2 antiseptic challenge test results for Ganoderma mouth rinses
From the data in table 2, it can be seen that the ganoderma lucidum mouthwash can pass the antiseptic challenge excellently, the antiseptic ability of the ganoderma lucidum mouthwash is basically equivalent to that of a pure chemical antiseptic, and the ganoderma lucidum mouthwash has good stability.
3. Bacteriostatic action of ganoderma lucidum mouthwash
Grouping experiments:
negative control group: agar plates without liquid medicine; positive control group: the drug concentrations are respectively: 1. 1/2, 1/4, 1/8, 1/16 times of the concentration of a certain brand of mouthwash (ingredients: thymol, eucalyptol, methyl salicylate, menthol) on medicated agar plates; experimental groups: the drug concentrations are respectively: 1. 1/2, 1/4, 1/8, 1/16 times the concentration of the medicated agar plates of the ganoderma mouth rinses of examples 1, 2 and 3.
And (3) MIC value determination:
the in vitro antibacterial activity of the ganoderma mouth wash on 3 strains of clinically common pathogenic bacteria dominant bacteria in the oral cavity is determined by adopting an Agar dilution method (Agar dilution method), and is expressed by Minimum Inhibitory Concentration (MIC). Taking experimental bacterial liquid (bacterial concentration: 1.5X 10)8/mL), spotted on the surface of agar plates containing different concentrations of medicine, and cultured for 48h in an anaerobic way (80% N)2+10％CO2+10％H2) Thereafter, MIC values were read.
And (4) analyzing results:
in the negative control group (blank group), all the experimental bacteria grew well, and the MIC values of the drug aqueous solutions of the positive control group and the experimental group can be included in the result analysis, which is shown in table 3.
TABLE 3 MIC values (times) of Ganoderma mouth rinses against 3 oral bacteria
As can be seen from Table 3, the MIC values of the ganoderma lucidum mouthwash against 3 oral bacteria are all smaller than those of the positive control group, i.e., the inhibition effect of the ganoderma lucidum mouthwash against Streptococcus mutans, Lactobacillus acidophilus and Porphyromonas gingivalis is stronger than that of the mouthwash common in the market. The minimum MIC value of the ganoderma lucidum mouthwash to 3 strains of pathogenic bacteria dominant bacteria common in oral clinic can reach 1/8 concentration, namely the ganoderma lucidum mouthwash still has a certain inhibition effect on streptococcus mutans and lactobacillus acidophilus after being diluted to 8 times, and still has a certain inhibition effect on porphyromonas gingivalis after being diluted to 4 times.
4, treatment effect of ganoderma lucidum mouthwash on rat oral ulcer
Establishing a dental ulcer model:
anesthetizing a rat with 2% sodium pentobarbital, burning NaOH crystals at mucous membranes of bilateral cheeks of the rat close to oral corners for 60s, washing with normal saline, and observing after 30 min:
experimental grouping and course of treatment:
52 rats were randomly divided into ganoderma mouth wash experimental groups (examples 1, 2 and 3), control group and blank group (no ulcer), 10 per group, and the remaining 2 model groups. After the modeling was successful for 24h, observations were made at 24, 48, 72 and 96h and at the end of healing, respectively, during which oral mucosal applications were performed daily. The experimental groups respectively adsorb 0.5mL of corresponding liquid medicine with cotton swab, and the control group is administered 0.5mL of 0.9% sodium chloride solution, and applied on the oral ulcer part of rat for 5 min. Blank and model groups were left untreated. 2 rats were harvested at 72h per group, the remaining rats were sacrificed at the time of substantial healing of the ulcer, and the mucosa at the oral ulcer was harvested and the healing time of each group was recorded.
And (4) analyzing results:
the healing time of the oral ulcer of the groups of examples 1, 2 and 3 is obviously shortened compared with that of the oral ulcer of the control group (P < 0.01); the difference in the healing time of canker sores between groups 1 and 2 was not statistically significant (P > 0.05). See fig. 1.
As can be seen from figure 1, the ganoderma lucidum mouthwash has an obvious effect of shortening the healing time of the dental ulcer and the influence of the ganoderma lucidum mouthwash on the tissue repair change of the dental ulcer of a rat:
the hematoxylin-eosin staining (HE staining) results show: after 72 hours of administration, the superficial epithelium and the superficial lamina propria of the focal region of the groups of examples 1 and 2 are necrosed, the tissue edema is obvious, inflammatory cells such as phagocyte, lymphocyte and the like are infiltrated in a large quantity, and the new granulation tissue is rich in blood vessels and is formed; example 3 group of lamina propria essentially necrosed, with extensive infiltration of inflammatory cells; necrosis of epithelium and lamina propria of ulcer area of control group, a large amount of infiltration of inflammatory cells, formation of new granulation tissue and abundant capillary vessels, degeneration and necrosis of muscle cells of muscular layer; the oral mucosa of the rats in the blank group is normal mucosa without ulcer, the epithelium and the lamina propria are continuous and have certain thickness, and tissue necrosis, cell edema, inflammatory cell infiltration and the like do not exist; in the ulcer area of the model group, partial epithelial lysis and continuous disappearance can be seen, edema of a connective tissue layer and massive infiltration of neutrophils, monocytes and macrophages can be seen. The granulation tissues of the groups of examples 1, 2 and 3 grew well after 72h of administration, and the healing degree was not obviously different under a light microscope; the local inflammatory symptoms of the ulcer of the control group are obvious, and the healing is poor.
Through a large number of experiments and analysis, when the formula of the ganoderma mouth wash consists of 100-200 parts of ganoderma extract, 35-45 parts of erythritol, 0.1-0.2 part of stevioside, 4070.4-0.6 part of poloxamer, 5-10 parts of fresh flower absolute oil and 745-860 parts of purified water, all raw materials in the formula play a role together, so that the ganoderma mouth wash has a good inhibition effect on streptococcus mutans, lactobacillus acidophilus and porphyromonas gingivalis, can effectively reduce local inflammatory reaction and promote tissue repair, and can effectively shorten the healing time of oral ulcer.
Claims (7)
1. The ganoderma lucidum mouthwash is characterized by comprising the following raw materials in parts by weight: 100-200 parts of ganoderma lucidum extract, 35-45 parts of erythritol, 0.1-0.2 part of stevioside, 4070.4-0.6 part of poloxamer, 5-10 parts of fresh flower absolute and 745-860 parts of purified water.
2. The ganoderma lucidum mouthwash according to claim 1, wherein the concentration of triterpenes and sterols in the ganoderma lucidum mouthwash is not less than 1mg/ml calculated as oleanolic acid.
3. The ganoderma lucidum mouthwash of claim 1, wherein the flower absolute is a mixture of one or more flower absolute.
4. The ganoderma lucidum mouthwash according to claim 3, wherein the flower absolute oil is a flower absolute oil of osmanthus fragrans or jasmine flowers, or a mixture of two flower absolute oils.
5. The ganoderma lucidum mouthwash according to claim 4, wherein the mixing ratio of the flower absolute oil of osmanthus fragrans or jasmine is 1-3: 3-1.
6. The ganoderma lucidum mouthwash according to claim 1, wherein the ganoderma lucidum extract is prepared by a method comprising:
6.1 crushing dried lucid ganoderma sporocarp with the water content less than or equal to 6 percent, sieving the crushed lucid ganoderma sporocarp with a 20-mesh sieve to obtain lucid ganoderma particles, adding 75 percent ethanol with the mass 10 times of that of the lucid ganoderma particles into the lucid ganoderma particles, extracting the lucid ganoderma particles for 2 hours at 80 ℃, filtering the lucid ganoderma particles by a 200-mesh sieve to obtain filtrate, adding 75 percent ethanol with the mass 10 times of that of the lucid ganoderma particles into filter residues again, extracting the lucid ganoderma particles for 2 hours at 80 ℃, filtering the lucid ganoderma particles by a 200-mesh sieve, combining the two extracted filtrates, concentrating, drying, crushing, and sieving the lucid ganoderma particles;
6.2 adding 95% ethanol 8 times the mass of the coarse powder of the ganoderma extract into the coarse powder of the ganoderma extract, dissolving, adding 10% dipotassium hydrogen phosphate aqueous solution 20 times the mass of the coarse powder of the ganoderma extract, shaking for extraction for 10min, standing for 2h, and taking the lower phase to obtain the ganoderma extract.
7. A method of preparing the ganoderma lucidum mouthwash of claim 1, wherein:
7.1. weighing fresh flower absolute oil and poloxamer 407 according to the formula proportion, and stirring to form uniform oily solution S1;
7.2. weighing erythritol, stevioside and lucid ganoderma extract according to the proportion of the formula, and stirring to form a uniform aqueous solution S2;
7.3. slowly dropwise adding the S1 into the S2 under the stirring state, uniformly mixing, adding purified water, and uniformly mixing and stirring;
7.4. stopping stirring, standing at 4 deg.C for 24 hr, filtering with 0.22 μm organic membrane for sterilization, and packaging to obtain Ganoderma collutory.
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| CN104188889A (en) * | 2014-08-27 | 2014-12-10 | 仙芝科技(福建)股份有限公司 | Lucid ganoderma toothpaste |
| CN104490742A (en) * | 2014-11-25 | 2015-04-08 | 张家港保税区冠祥贸易有限公司 | Cavity health care cleaning fluid and producing method thereof |
| CN110075134A (en) * | 2019-04-26 | 2019-08-02 | 四川省农业科学院土壤肥料研究所 | A kind of extracting method of ganodenic acid acid |
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| CN103695175A (en) * | 2013-12-13 | 2014-04-02 | 仙芝科技(福建)股份有限公司 | Method for extracting and separating components of osmanthus flower or arabian jasmine flower |
| CN104188889A (en) * | 2014-08-27 | 2014-12-10 | 仙芝科技(福建)股份有限公司 | Lucid ganoderma toothpaste |
| CN104490742A (en) * | 2014-11-25 | 2015-04-08 | 张家港保税区冠祥贸易有限公司 | Cavity health care cleaning fluid and producing method thereof |
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