CN112816624A - Waterlogging stress test method - Google Patents
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- CN112816624A CN112816624A CN202011644452.5A CN202011644452A CN112816624A CN 112816624 A CN112816624 A CN 112816624A CN 202011644452 A CN202011644452 A CN 202011644452A CN 112816624 A CN112816624 A CN 112816624A
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Abstract
The invention relates to the technical field of agricultural information, in particular to acquisition and analysis of information in waterlogging stress tests. The method for acquiring and analyzing information in the waterlogging stress test comprises a waterlogging stress test barrel, specifically comprises an inner barrel for planting plants and an outer barrel for accommodating the inner barrel; a partition board which partitions the outer barrel into an upper layer and a lower layer is arranged in the outer barrel and close to the bottom of the outer barrel, a positioning hole for positioning the inner barrel is formed in the center of the partition board, and water outlets are formed in the upper layer bottom and the lower layer bottom of the outer barrel; according to the invention, the outer barrel is injected with water, so that the cultivation of plants in the cultivation barrel and waterlogging stress test are realized, and the influence of waterlogging stress on the plants is researched by acquiring the growth distribution condition of the root system and the secretion of the root system in waterlogging water, so that technical support is provided for screening waterlogging-resistant plant varieties.
Description
Technical Field
The invention relates to the technical field of agricultural information, in particular to acquisition and analysis of information in waterlogging stress tests.
Background
The waterlogging stress disaster is caused by the fact that water is accumulated on the ground of a farmland due to continuous larger rainfall, air in soil is lack, air permeability is poor, oxygen exchange between the soil and the atmosphere is difficult, production of crops (such as wheat, corn, peanut and rape) is seriously influenced, yield and quality are reduced, therefore, research on influence of waterlogging stress on plants and improvement on waterlogging resistance of the plants are necessary, but a related test device suitable for researching the waterlogging stress of the plants does not exist at the present stage, so that a waterlogging stress test method is urgently needed to be designed
Disclosure of Invention
The invention aims to provide a waterlogging stress method for a plant.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a waterlogging stress test method is characterized in that: comprises an inner barrel for planting plants and an outer barrel for accommodating the inner barrel;
a partition board which partitions the outer barrel into an upper layer and a lower layer is arranged in the outer barrel and close to the bottom of the outer barrel, a positioning hole for positioning the inner barrel is formed in the center of the partition board, and water outlets are formed in the upper layer bottom and the lower layer bottom of the outer barrel;
the bottom of the inner barrel is embedded into a positioning hole in the outer barrel and is sealed with the partition plate, and the upper layer space and the lower layer space of the outer barrel form two independent spaces;
when the inner barrel is accommodated in the outer barrel, the height of the opening part of the inner barrel is lower than that of the opening part of the outer barrel;
the test method comprises the following steps:
s1, filling the inner barrel with a nutrient medium, and planting plants for waterlogging stress test in the inner barrel;
s2, when waterlogging stress test is carried out on the planted plants, the inner barrel is placed in the outer barrel; and is positioned by a central positioning groove;
s3, injecting water into the outer barrel until the water submerges the surface layer of the inner barrel substrate by 5-10cm, and forming waterlogging stress on plants; waterlogging stress for 5-7 days;
s4, after the waterlogging stress time is up, opening a water outlet, and discharging water in the inner barrel and the outer barrel to relieve the waterlogging stress;
s5, after waterlogging stress is relieved, continuously culturing the plants to grow for 15-20 days;
s6, after the cultivation is finished, taking out the inner barrel, and opening the movable side wall of the inner barrel; and judging waterlogging stress tolerance of the plant through observed growth distribution and root length of the plant root system.
Preferably, a movable side wall is vertically arranged on the barrel wall of the inner barrel, and the movable side wall is detachably and hermetically connected to the barrel wall of the inner barrel; for realizing the opening and closing of the vertical opening of the barrel wall of the inner barrel
Preferably, the method comprises the following steps: the inner barrel is a circular truncated cone; or the inner barrel is a cylinder, and when the inner barrel is the cylinder, the positioning hole is a circular table hole with a wide upper part and a narrow lower part.
Preferably, a water outlet at the bottom of the outer barrel is provided with a flow meter.
Preferably, the wall of the outer barrel at the lower layer is made of transparent material.
Preferably, the movable side wall is of an arc plate structure with the cross sections at two ends in a convex shape, and the movable side wall is matched with the side groove structure of the fixed side wall in size, so that the movable side wall is tightly matched with the fixed side wall; and the movable side wall is clamped into the matched groove structure on the barrel bottom.
Preferably, the inner barrel is respectively provided with sensors for detecting oxygen content, temperature, humidity, pH value and conductivity in soil on layers with different heights.
Preferably, the height direction of the barrel body of the inner barrel and the outer barrel is provided with corresponding scales.
Preferably, the bottom of the inner barrel is tightly matched with the spacing layer on the outer barrel through a circular truncated cone groove, and the inner barrel is used for positioning and placing the inner barrel and plugging the circular truncated cone groove.
Preferably, a flow meter is installed at the bottom of the outer barrel; the flowmeter is arranged on a water outlet pipe at the bottom of the outer barrel.
Preferably, the bottom of the outer barrel is an inclined plane, and the bottom end of the outer barrel is close to the water outlet of the water outlet pipe.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the inner barrel with the water outlet hole at the bottom is arranged in the accommodating cavity of the outer barrel, and the inner barrel and the outer barrel are watered, so that the cultivation of plants in the cultivation barrel and the waterlogging stress test are realized, and the influence of waterlogging stress on the plants is researched; the detachable movable side wall is arranged in the height direction of the inner barrel, the vertical side edge of the inner barrel from top to bottom is used for opening the growth of the underground part of the plant, and the growth and development conditions of the root system and the culture medium from top to bottom can be directly observed, sampled and detected through the vertical side opening; the opening at the side edge is used for directly washing the root system, so that a relatively complete plant root system is obtained; the side-open inner barrel effectively ensures the integrity of the root system and improves the accuracy of measuring the root length, the root density and the root volume; compared with the traditional pot culture barrel, the pot culture barrel reduces the damage to the root system, and can more accurately reflect the influence of waterlogging stress on the activity of enzyme related to the root system.
2. The invention can calculate the water yield in the water outlet pipe by arranging the flowmeter, and can simulate the surface water seepage amount in field production by controlling the water flow speed, so that the waterlogging stress test is more similar to the waterlogging stress disaster in field production, the reliability of the plant waterlogging stress test is improved, and the design is more reasonable.
3. The device has the advantages of simple structure, convenient installation and operation, repeated utilization, economy, practicality, flexible use, reasonable and ingenious design and compact structure.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a top view of an embodiment of the present invention;
FIG. 3 is a front view of an embodiment of the present invention;
FIG. 4 is an enlarged view of a circular truncated cone groove mounting structure of an inner barrel and an outer barrel according to an embodiment of the present invention;
FIG. 5 is a schematic view of an inner barrel according to an embodiment of the present invention;
FIG. 6 is a schematic structural diagram of an outer tub according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of an exploded structure of an embodiment of the present invention;
FIG. 8 is a cross-sectional view of the inner barrel at a 40cm scale position in an embodiment of the present invention;
FIG. 9 is a schematic structural diagram of another embodiment of the present invention;
FIG. 10 is a front view of another embodiment of the present invention;
the reference numbers in the figures illustrate:
1. an inner barrel; 11. a fixed side wall; 12. a movable side wall; 13. a barrel bottom; 14. a water outlet hole; 2. an outer tub; 21. a barrel body; 22. a spacer layer; 221. a circular platform groove; 23. a water outlet pipe; 24. a bevel; 3. plant growing; 4. an Internet of things wireless sensor; 5. a flow meter; 6. and (4) switching on and off the valve.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
With the attached drawings 1-10, the invention provides a waterlogging stress test device for a plant.
In the first embodiment, with reference to fig. 1 and fig. 2, the plant cultivating device comprises an inner barrel 1 for planting plants to be waterlogging stress test, and an outer barrel 2 for accommodating the inner barrel 1;
the inner barrel 1 comprises a fixed side wall 11, a movable side wall 12 and a barrel bottom 13; the side edge of the fixed side wall 11 is provided with a vertical opening from the wall top to the barrel bottom 13, and the movable side wall 12 is detachably arranged on the vertical opening and is used for realizing the opening and closing of the vertical opening of the barrel wall of the inner barrel 1; a water outlet hole 14 is formed in the barrel bottom 13;
the inner barrel 1 is arranged in the outer barrel 2, and the top of the inner barrel 1 is lower than that of the outer barrel 2.
According to the invention, the inner barrel with the water outlet hole at the bottom is arranged in the accommodating cavity of the outer barrel, and water is injected into the inner barrel and the outer barrel, so that the cultivation of plants in the cultivation barrel and waterlogging stress test are realized, and the influence of waterlogging stress on the plants is researched; the detachable movable side wall is arranged in the height direction of the inner barrel, the vertical side edge of the inner barrel from top to bottom is used for opening the growth of the underground part of the plant, and the growth and development conditions of the root system and the culture medium from top to bottom can be directly observed, sampled and detected through the vertical side opening; the opening at the side edge is used for directly washing the root system, so that a relatively complete plant root system is obtained; the side-open inner barrel effectively ensures the integrity of the root system and improves the accuracy of measuring the root length, the root density and the root volume; compared with the traditional pot culture barrel, the pot culture barrel reduces the damage to the root system, and can more accurately reflect the influence of waterlogging stress on the activity of enzyme related to the root system.
Preferably, the inner barrel 1 and the outer barrel 2 are both of a barrel-shaped structure with an opening at the upper part; the fixed side wall 11 is fixedly arranged on the edge of the barrel bottom 13.
With reference to fig. 3, the outer barrel 2 is further provided with a spacing layer 22 for placing the inner barrel 1; the spacing layer 22 is also provided with a spacing layer 22 along the height direction of the outer barrel; the inner barrel 1 is arranged on the spacing layer 22.
Sensors for detecting oxygen content, temperature, humidity, pH value and conductivity in soil are respectively arranged on layers with different heights in the inner barrel 1; preferably, a plurality of internet of things wireless sensors 4 are uniformly arranged on layers with different heights on the inner barrel 1, and the change conditions of oxygen content, temperature, humidity, pH value and conductivity in soil can be collected in real time by installing the plurality of internet of things wireless sensors 4 at different soil depths of the inner barrel 1, so that a theoretical basis is provided for the growth and development states of the plants 3.
Corresponding scales are arranged in the height direction of the barrel bodies of the inner barrel 1 and the outer barrel 2, so that the growth distribution range of the root system of the plant 3 in the soil can be conveniently observed; the scales on the inner barrel 1 and the outer barrel 2 are correspondingly arranged, and the wireless sensor 4 of the internet of things is respectively positioned at corresponding positions in the inner barrel 1, so that the data related to soil layers with different depths where the 3 root systems of the plants are located can be acquired, and the growth and development conditions of the root systems of the plants 3 in the soil with different depths and the consumption conditions of soil nutrients can be conveniently known.
Referring to fig. 4, the bottom of the inner barrel 1 is tightly fitted with the spacing layer 22 on the outer barrel 2 through a circular platform groove 221 for positioning the inner barrel 1; the inner barrel 1 is tightly matched with the positioning groove on the spacing layer 33 through the barrel bottom 13, the inner side of the inner barrel 1 is communicated with the hollow cavity at the lower part of the spacing layer 22 of the outer barrel 2 through the water outlet hole 14, and the upper spaces of the inner barrel 1 and the spacing layer 22 of the outer barrel 2 form an annular cavity structure with an upper opening, so that two water paths of the device in the test process are formed.
Preferably, the bottom outer side edge chamfer structure of the inner barrel 1 is matched with the size of the circular truncated cone groove 221, the inner barrel 1 is directly placed in the circular truncated cone groove 221, the inner barrel 1 and the outer barrel 2 are positioned and placed, the inner barrel 1 and the outer barrel are conveniently and quickly assembled, the structure is simple, and the processing is convenient.
Preferably, the circular truncated cone groove 221 is an open groove-shaped structure; the spacer layer 22 is of a circular ring-shaped plate structure, the bottom end of the assembled inner barrel 1 is flush with the bottom end of the spacer layer 22, meanwhile, the scales of the height directions of the inner barrel 1 and the outer barrel are correspondingly arranged, the positioning assembly is convenient and fast, the whole structure is simpler, the structure is compact, the design is ingenious, and the processing is convenient.
With reference to fig. 5, the movable side wall 12 is an arc-plate structure; the left end and the right end of the movable side wall 12 are closely matched and connected with the opening of the fixed side wall 11, the movable side wall 12 is closely matched and connected with the barrel bottom 13, and the containing space of the barrel body is used for effectively cultivating the plant 3.
Preferably, the movable side wall 12 is an arc plate structure with convex cross sections at two ends, and is matched with the side groove structure of the fixed side wall 11 in size, so that the movable side wall 12 is tightly matched with the fixed side wall 11; and the movable side wall 12 is clamped into a matched groove structure on the barrel bottom 13; the movable side wall 12 can be quickly disassembled and assembled, and meanwhile, the leakage of silt and water is reduced through the bent waterway structure; simple and compact structure and reasonable design.
Referring to fig. 6, the outer tub 2 includes a tub 21 and a partition 22; the spacing layer 22 is arranged at intervals with the barrel bottom along the height direction of the barrel body 21; it should be noted that, by arranging the spacing layer, the suspended support of the inner barrel 1 can be realized, and the water level in the outer barrel can be conveniently controlled to be used for waterlogging stress tests on the inner barrel 1.
The spacing layer 22 is an annular plate structure.
Preferably, the inner barrel 1 and the outer barrel 2 are made of transparent materials, so that the test condition can be directly observed conveniently; during a specific test, the peripheries of the inner barrel 1 and the outer barrel 2 are wrapped with tin foil paper, so that the root system growing environment is kept dark; the inner barrel 1 and the outer barrel 2 are coaxially positioned, and the barrel opening of the inner barrel 1 is 15cm lower than the barrel opening of the outer barrel 2.
A water outlet pipe 23 is further arranged at the bottom of the outer barrel 2, a switch valve 6 is further arranged on the water outlet pipe 23, and the switch valve 6 is arranged for switching on and off the water outlet at the bottom of the outer barrel 2; waterlogging stress can be relieved through the switch valve 6; the switch valve 6 is matched with the flowmeter 5, so that the water flow speed can be adjusted, and the simulation of surface water leakage is realized; the water outlet pipe 23 is opened through the switch valve 6, so that the root exudates of wheat can be collected when waterlogging stress occurs, and sampling detection is performed; meanwhile, the water outlet pipe 23 is opened through the switch valve 6, so that impurities such as soil in the outer barrel 2 can be washed and flow out of the water outlet pipe 23.
The device has the advantages of simple structure, convenient installation and operation, repeated utilization, economy, practicality, flexible use, reasonable and ingenious design and compact structure.
With reference to fig. 7, the experimental method for studying waterlogging stress of plants comprises the following steps:
firstly, sowing plants 3 in an inner barrel 1, positioning and placing an Internet of things wireless sensor 4, placing the inner barrel 1 in an outer barrel 2, and shading the barrel body of the outer barrel 2;
step two, normally watering and culturing under the greenhouse condition;
thirdly, when the plant 3 reaches the growth stage required by the test scheme, waterlogging stress is carried out;
step four, after keeping the waterlogging stress state of the plant 3 for a plurality of days, taking out the inner barrel 1 to remove the waterlogging stress;
and fifthly, shading the barrel body of the inner barrel 1, culturing the plants for 3 days under normal conditions, and then sampling and investigating the underground parts of the plants and the culture medium thereof.
In the concrete implementation, because waterlogging stress is an important disaster affecting wheat production in the rice and wheat rotation area, the waterlogging stress affects the growth and development of wheat, inhibits physiological and biochemical metabolism and substance transfer of the wheat, further reduces yield and quality, provides a theoretical basis for the waterlogging-resistant cultivation of wheat in the rice and wheat rotation area, summarizes related researches of scholars at home and abroad on the aspect of wheat waterlogging resistance in recent years, and the consistent results show that breeding of waterlogging-resistant varieties, exercising of waterlogging stress in seedling stage, reasonable fertilization and spraying of exogenous regulating substances are one of the main technical measures for improving the wheat waterlogging resistance. Therefore, the wheat is taken as a test object, and has important significance in waterlogging stress research.
Preparing a transparent inner barrel 1 and an outer barrel 2 in advance, wherein scales are arranged on the outer side walls of the inner barrel 1 and the outer barrel 2 along the height direction, the inner barrel 1 is respectively provided with scales (0 cm, 5cm, 10cm, 15cm, 20cm, 25cm, 30cm, 35cm, 40cm, 45cm and 50cm) from top to bottom, the inner barrel 1 is placed on a circular platform groove 221 on a spacing layer 22 of the outer barrel 2, and the inner barrel 1 and the outer barrel 2 are tightly matched for positioning and placing the inner barrel 1; scales (0, 5cm, 10cm, 15cm, 20cm, 25cm, 30cm, 35cm, 40cm, 45cm and 50cm) on the outer barrel 2 are respectively arranged corresponding to the scales on the inner barrel 1 one by one, and the scales arranged corresponding to the inner barrel 1 and the outer barrel 2 one by one are used for realizing accurate control of waterlogging depth in the test.
With reference to fig. 8, the internet of things wireless sensors 4 are respectively arranged at different heights (10cm, 20cm, 30cm, 40cm and 50cm) in the inner barrel 1; 5 wireless sensors of the internet of things are uniformly arranged on horizontal position surfaces with different heights (10cm, 20cm, 30cm, 40cm and 50cm) in the inner barrel 1 respectively.
First, seeding is performed. Uniformly mixing fertilizer and soil, then loading the mixture into an inner barrel 1, then placing the inner barrel 1 into an outer barrel 2, and filling soil around the inner barrel 1, wherein the soil depth in the inner barrel 1 is consistent with that in the outer barrel 2; the body of the outer barrel 2 is wrapped by tinfoil paper; wheat seeds are sown into the inner barrel 1, 5 grains are sown in each barrel, and the watering is carried out normally.
Secondly, when the wheat has five leaves and one heart, dividing the test material into two groups, wherein one group is normally Cultured (CK), the other group is stressed by waterlogging stress (T), the water depth is kept to be 5cm, the stress lasts for 7 days, the side opening of the inner barrel 1 is opened, and the growth and development conditions of the whole root system, namely the distribution range of the root system in the soil (the distribution ratio of the root system in the shallow layer, the middle layer and the deep layer of the soil) are observed. The root system was then flushed with tap water, root length, root density and root volume were measured, and young roots were taken to test their stress-related enzyme activities (SOD, POT, CAT and MDA).
It should be noted that the wireless sensor 4 of the internet of things can monitor the change conditions of temperature, humidity, oxygen content, pH value, conductivity and the like in the culture medium in real time, check numerical information through the internet of things, store and analyze data, and provide theoretical support for the growth and development of plants in the inner barrel and waterlogging stress conditions.
In another embodiment, referring to fig. 9 and 10, unlike the above embodiment, a flowmeter 5 is installed at the bottom of the outer tub 2; the flowmeter 5 is arranged on a water outlet pipe 23 at the bottom of the outer barrel 2.
This application can calculate the water yield in outlet pipe 23 through setting up flowmeter 5, and accessible control water velocity and then the seepage volume of surface water in the simulation field production, makes the waterlogging of waterlogging coerce experiment more similar with the waterlogging coerce calamity in the field production, improves the experimental reliability of plant waterlogging coerce, and the design is more reasonable.
The water outlet pipe 23 is also provided with a switch valve 6, and the switch valve 6 is used for switching on and off the water outlet at the bottom of the outer barrel 2; waterlogging stress can be relieved through the switch valve 6; the switch valve 6 is matched with the flowmeter 5, so that the water flow speed can be adjusted, and the simulation of surface water leakage is realized; the water outlet pipe 23 is opened through the switch valve 6, so that the root exudates of wheat can be collected when waterlogging stress occurs, and sampling detection is performed; meanwhile, the water outlet pipe 23 is opened through the switch valve 6, so that impurities such as soil in the outer barrel 2 can be washed and flow out of the water outlet pipe 23.
In another embodiment, the bottom of the outer tub 2 is an inclined plane 24, and the bottom end of the outer tub is close to the water outlet of the water outlet pipe 23, and the height of the bottom of the inclined plane is higher than the height of the bottom of the inner side wall of the water outlet pipe 23, so that the retention of water can be effectively prevented, and the accuracy of the flow meter 5 is improved.
The test method for researching waterlogging stress of the plants in the embodiment comprises the following steps:
firstly, sowing plants 3 in an inner barrel 1, positioning and placing an Internet of things wireless sensor 4, placing the inner barrel 1 in an outer barrel 2, and shading the barrel body of the outer barrel 2;
step two, normally watering and culturing under the greenhouse condition;
thirdly, when the plant 3 reaches the growth stage required by the test scheme, waterlogging stress is carried out; periodically collecting waterlogging stress water samples through a switch valve 6 to detect the components of plant root exudates;
step four, after waterlogging stress treatment is finished, discharging water on the surface layer of soil in the inner barrel 1 through the switch valve 6 to relieve waterlogging stress;
and step five, after waterlogging stress is finished, regulating the water flow through a flowmeter 5 and a switch valve 6, simulating the surface water leakage amount of the field, culturing the plants for 3 days under normal conditions, and then sampling and researching the plants and the culture medium.
In specific implementation, firstly, seeding is carried out. Uniformly mixing fertilizer and soil, then loading the mixture into an inner barrel 1, then placing the inner barrel 1 into an outer barrel 2, and filling soil around the inner barrel 1, wherein the soil depth in the inner barrel 1 is consistent with that in the outer barrel 2; the body of the outer barrel 2 is wrapped by tinfoil paper; wheat seeds are sown into the inner barrel 1, 5 grains are sown in each pot, and normal watering is carried out.
Secondly, when the wheat has five leaves and one heart, dividing the test material into two groups, wherein one group is normally Cultured (CK), the other group is subjected to waterlogging stress (T), the waterlogging stress treatment of the plant 3 in the inner barrel 1 is realized by closing the switch valve 6, a watering can is used for watering, the water depth of the soil surface layer is 5cm, and the waterlogging stress treatment is carried out for 7 days. After waterlogging stress treatment is finished, the switch valve 6 is opened to discharge excessive water on the surface layer of the soil in the inner barrel 1, the flow of water is adjusted through the switch valve 6 and the flowmeter 5 to simulate the field surface leakage amount, plants are cultured in a natural state to recover the growth of the plants, and the underground parts of the plants are sampled and investigated after 20 days.
It should be noted that, in the cultivation of wheat, the root system of wheat can produce water-soluble secretions (such as soluble sugar, reducing sugar, free amino acid and secondary metabolites), and the water body of the plant root system under different waterlogging stress time (1 day, 3 days, 5 days and 7 days) is collected by opening the switch valve 6 for sampling research, so that the design is more reasonable.
Finally, acquiring the change conditions of oxygen content, temperature, humidity, pH value and conductivity in the soil in real time through the wireless sensor 4 of the Internet of things; after culturing for 20 days under normal conditions, the two test treatment groups are respectively taken out from the outer barrel 2, the side opening of the inner barrel 1 is opened, and the growth and development conditions of the whole root system, namely the distribution range of the root system in the soil (the distribution proportion of the root system in the shallow layer, the middle layer and the deep layer of the soil) are observed. And then washing the side opening of the inner barrel 1 with tap water to obtain a complete root system, measuring the root length, the root density and the root volume, and taking the young root to detect the activity (SOD, POT, CAT and MDA) of the enzyme relevant to the stress.
It should be noted that the detailed description of the invention is not included in the prior art, or can be directly obtained from the market, and the detailed connection mode can be widely applied in the field or daily life without creative efforts, and the detailed description is not repeated here.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (7)
1. A test method of the waterlogging stress of the plants comprises a test barrel of the waterlogging stress of the plants, and is characterized in that the test barrel comprises an inner barrel for planting the plants and an outer barrel for accommodating the inner barrel;
a partition board which partitions the outer barrel into an upper layer and a lower layer is arranged in the outer barrel close to the bottom, water seepage holes are distributed in the partition board, and a positioning hole for positioning the inner barrel is arranged in the center of the partition board;
the bottom of the inner barrel is embedded into a positioning hole in the outer barrel and is sealed with the partition plate, and the upper layer space and the lower layer space of the outer barrel form two independent spaces;
the bottom of the outer barrel is respectively provided with a water outlet; the bottom of the inner barrel is provided with a water seepage hole;
when the inner barrel is accommodated in the outer barrel, the height of the opening part of the inner barrel is lower than that of the opening part of the outer barrel.
The test method comprises the following steps:
s1, filling the inner barrel with a nutrient medium, and planting plants for waterlogging stress test in the inner barrel;
s2, when waterlogging stress test is carried out on the planted plants, the inner barrel is placed in the outer barrel; and is positioned by a central positioning groove;
s3, injecting water into the outer barrel until the water submerges the surface layer of the inner barrel substrate by 5-10cm, and forming waterlogging stress on plants; waterlogging stress for 5-7 days;
s4, after the waterlogging stress time is up, opening a water outlet, and discharging water in the inner barrel and the outer barrel to relieve the waterlogging stress;
s5, after waterlogging stress is relieved, continuously culturing the plants to grow for 15-20 days;
s6, after the cultivation is finished, taking out the inner barrel, and opening the movable side wall of the inner barrel; and judging waterlogging stress tolerance of the plant through observed growth distribution and root length of the plant root system.
2. The method for testing waterlogging stress of a plant according to claim 1, further comprising the steps of:
during S3 waterlogging stress, opening a lower water outlet flow meter valve; collecting flowing water, analyzing root secretion in the flowing water, and analyzing the growth condition of the root system during waterlogging.
3. The waterlogging stress test method as claimed in claim 1, wherein the inner barrel has a movable side wall along the vertical direction, and the movable side wall is detachably and hermetically connected to the inner barrel wall; the inner barrel is used for realizing the opening and closing of the vertical opening of the barrel wall of the inner barrel.
4. The waterlogging stress test method as claimed in claim 1, wherein: the inner barrel is a circular truncated cone; or the inner barrel is a cylinder, and when the inner barrel is the cylinder, the positioning hole is a circular table hole with a wide upper part and a narrow lower part.
5. The waterlogging stress test method as recited in claim 1, wherein each of the water outlets is provided with a flow valve.
6. The waterlogging stress test method as claimed in claim 4, wherein the lower wall of the outer barrel is made of transparent material.
7. The plant waterlogging stress test apparatus according to claim 1, wherein: the movable side wall is of an arc plate structure with convex cross sections at two ends and is matched with the side groove structure of the fixed side wall in size, so that the movable side wall is tightly matched with the fixed side wall; and the movable side wall is clamped into the matched groove structure on the barrel bottom.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040237402A1 (en) * | 2001-09-07 | 2004-12-02 | Rune Nielsen | Capilar controlled watering of plants |
| US20090199473A1 (en) * | 2008-02-13 | 2009-08-13 | Watson Jr William J | Horticultural Growth Medium |
| CN106105753A (en) * | 2016-08-08 | 2016-11-16 | 云南省农业科学院甘蔗研究所 | A kind of Caulis Sacchari sinensis barrel plant drought stress test method |
| CN206808169U (en) * | 2017-03-24 | 2017-12-29 | 浙江家乐蜜园艺科技有限公司 | A kind of Planting barrel of observable root growth |
| CN108142272A (en) * | 2018-03-01 | 2018-06-12 | 中国科学院遗传与发育生物学研究所 | For the interim water stress device and method of water planting potted plant crop |
| CN108713493A (en) * | 2018-07-30 | 2018-10-30 | 安徽农业大学 | For analyzing the water planting potting bucket for heating and being influenced on wheat root distribution |
| CN109349073A (en) * | 2018-12-18 | 2019-02-19 | 中国科学院沈阳应用生态研究所 | A kind of plant alternate irrigation simulation test device and method |
| CN109819818A (en) * | 2018-12-29 | 2019-05-31 | 北京市农林科学院 | Adversity-stressed Plant earth pillar casing and its application method and application |
| CN209749267U (en) * | 2019-04-16 | 2019-12-10 | 山东农业工程学院 | Detachable multifunctional plant root observation barrel |
-
2020
- 2020-12-31 CN CN202011644452.5A patent/CN112816624B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040237402A1 (en) * | 2001-09-07 | 2004-12-02 | Rune Nielsen | Capilar controlled watering of plants |
| US20090199473A1 (en) * | 2008-02-13 | 2009-08-13 | Watson Jr William J | Horticultural Growth Medium |
| CN106105753A (en) * | 2016-08-08 | 2016-11-16 | 云南省农业科学院甘蔗研究所 | A kind of Caulis Sacchari sinensis barrel plant drought stress test method |
| CN206808169U (en) * | 2017-03-24 | 2017-12-29 | 浙江家乐蜜园艺科技有限公司 | A kind of Planting barrel of observable root growth |
| CN108142272A (en) * | 2018-03-01 | 2018-06-12 | 中国科学院遗传与发育生物学研究所 | For the interim water stress device and method of water planting potted plant crop |
| CN108713493A (en) * | 2018-07-30 | 2018-10-30 | 安徽农业大学 | For analyzing the water planting potting bucket for heating and being influenced on wheat root distribution |
| CN109349073A (en) * | 2018-12-18 | 2019-02-19 | 中国科学院沈阳应用生态研究所 | A kind of plant alternate irrigation simulation test device and method |
| CN109819818A (en) * | 2018-12-29 | 2019-05-31 | 北京市农林科学院 | Adversity-stressed Plant earth pillar casing and its application method and application |
| CN209749267U (en) * | 2019-04-16 | 2019-12-10 | 山东农业工程学院 | Detachable multifunctional plant root observation barrel |
Non-Patent Citations (3)
| Title |
|---|
| 任珊露等: "采用微型渗漏计原位测定设施菜田有机肥氮素矿化特征", 《中国蔬菜》 * |
| 马尚宇，刘雅男等: "花后渍水时长对小麦根系形态和抗氧化酶活性及产量的影响", 《麦类作物学报》 * |
| 马尚宇，韩笑，王艳艳等: "花后渍水对小麦根系形态、生理及地上部干物质分配和产量的影响", 《南京农业大学学报》 * |
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