CN112807355A

CN112807355A - A natural nontoxic deodorant liquid for preventing and treating loempe and foot odor, and its preparation method

Info

Publication number: CN112807355A
Application number: CN201911130604.7A
Authority: CN
Inventors: 李天华
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-11-18
Filing date: 2019-11-18
Publication date: 2021-05-18

Abstract

The invention relates to a natural nontoxic deodorant liquid for preventing and treating beriberi and foot odor and a preparation method thereof, wherein the deodorant liquid comprises the following components: 18 to 99 percent of clove and 82 to 1 percent of pepper, and the preparation steps are as follows: pulverizing fructus Zanthoxyli Diversifoliae into coarse powder to obtain fructus Zanthoxyli Diversifoliae powder, extracting with proper extraction method, decolorizing with proper decolorizing method to remove pigment, and making into natural nontoxic deodorant solution for treating loempe and foot odor. The decolorized deodorant liquid has little pollution to insoles and socks. The clove and the pepper are all natural edible materials, have no toxic or side effect and have high user acceptance. The deodorant liquid is sprayed on insoles and socks for preventing and treating beriberi and foot odor, the insoles can be placed in the shoes for a long time to play the roles of deodorization and bacteriostasis, the deodorant liquid has good treatment effect on the foot odor, the effective rate reaches 100%, and the deodorant liquid is simple, convenient, easy, nontoxic, safe and reliable in effect.

Description

A natural nontoxic deodorant liquid for preventing and treating loempe and foot odor, and its preparation method

[ technical field ] A method for producing a semiconductor device

The invention relates to a deodorant liquid for treating beriberi and foot odor and a preparation method thereof, in particular to a deodorant liquid for treating the bunge pricklyash peel and a preparation method thereof.

[ technical background ] A method for producing a semiconductor device

The foot odor is caused by the fact that sweat glands are abundant in the sole center, and sweat is easy to sweat when walking or exercising, and the sweat contains water, salt, lactic acid and urea. In a humid environment, bacteria and fungi on the feet multiply and decompose keratin, together with urea and lactic acid in the perspiration, thus emitting an odor. If the air permeability of the shoe is poor, the more concentrated the odor is, the more intense the odor is.

Beriberi, also causes foot odor. People with beriberi generally have symptoms of sweating, foot odor, foot itching and the like, and serious symptoms of skin falling, red swelling, blisters, cracks, fester and the like appear between the toe seams of patients.

The root of foot odor is foot skin which has much sweat and odor, but can develop into serious dermatophytosis after a long time.

The beriberi, commonly known as athlete's foot, is medically called tinea pedis and is caused by fungal infection, and is a very common, extremely infectious and easily relapsed foot dermatosis. Fungi invading the stratum corneum of humans or animals are called dermatophytes, among which are the genera trichophyton, microsporum and floccosum. Trichophyton rubrum has become the leading causative agent of tinea pedis in our country at present, because it is resistant and difficult to control.

According to the different symptoms, the disease can be divided into blister type, erosion type and scale keratosis type. The blister type symptoms are mainly small blisters, which are well developed on the soles and sides of the toes, accompanied by circlewise desquamation with obvious itching. Internally rubbing and eroding type takes the interdigital part as the main disease part, and the local part becomes white, erosive and fluid when scratching, and can cause complications such as erysipelas and the like due to the secondary bacterial infection caused by scratching. The scaly keratotic type is more common, and people who often have the scaly keratotic type are mostly of the scaly keratotic type, and are often characterized by local dryness, chapping and bleeding in winter.

Foot odor is an obvious symptom of beriberi, if beriberi is treated untimely, itching is easy to occur due to the beriberi, and fungal infection such as tinea manuum, tinea corporis, tinea capitis and the like is easy to generate at other parts of a body after scratching. More seriously, scratching causes local bacterial infection, which may become lymphangitis, cellulitis.

At present, more medicaments for treating beriberi in a pharmacy of a hospital are available, such as western medicine cream, western medicine spray, traditional Chinese medicine foot soaking powder, traditional Chinese medicine ointment and the like. The western medicine cream and the western medicine spray are medicines which need to be used under the guidance of doctors, some medicines have larger irritation and obvious curative effect, but the symptoms are not treated, the diseases are easy to recur after the medicines are stopped, and the medicine parts are itching, red and swollen, burning and pain caused by individual adverse reactions. The traditional Chinese medicine foot bath powder is troublesome to apply, needs to be dissolved by hot water firstly and then soaked for a long time, is complex in process, and has obvious curative effect on some foot bath powder added with salicylic acid by accelerating the metabolism of skin cells and accelerating the shedding of stratum corneum cells so as to integrally remove bacterial colonies. The traditional Chinese medicine ointment has good curative effect, but has heavy oiliness, particularly has dark color, and is easy to pollute socks, insoles and shoes.

When the medicines are used, the main using method is to apply, spray or soak the medicines, the main action part is the foot, but actually, the main cause of the beriberi is fungi, even if the fungi on the foot are killed or inhibited, the fungi exist in the used socks, insoles and shoes all the time, so the beriberi is easy to relapse.

[ summary of the invention ]

The invention aims to overcome the defects and prepare the deodorant liquid for treating beriberi and foot odor, wherein the deodorant liquid is sprayed on insoles, socks and feet, and the materials used are clove and pepper which are all edible materials, have no toxic or side effect and are all natural substances. The special method is adopted for decolorization treatment, so that the effective components can be retained, ineffective pigments can be removed, socks and insoles are not polluted, the substances for removing odor and inhibiting bacteria are sprayed on the insoles, and the insoles are placed in the shoes for a long time to play the roles of removing odor and inhibiting bacteria.

The materials of the invention comprise clove, pepper and other materials. Flos Caryophylli is the dried bud of flos Caryophylli of Myrtaceae. Picked up when the flower buds turn red from green, dried in the sun. Contains volatile oil, which contains eugenol, acetyl eugenol, caryophyllene, methyl n-pentanone, methyl n-heptanone, vanillin, and oleanolic acid, tannin, fatty oil and wax. The fructus Zanthoxyli is dry mature peel (fructus Zanthoxyli) and seed (semen Zanthoxyli) of Rutaceae plant green pepper (pericarpium Zanthoxyli, green fructus Zanthoxyli, and fructus Zanthoxyli) or fructus Zanthoxyli (pericarpium Zanthoxyli, fructus Capsici, fructus Carthami tinctorii, and radix Campylotropis Hirtella) as raw materials. Harvesting mature fruits in autumn, removing impurities and drying in the sun. The fructus Zanthoxyli fruit contains volatile oil containing geraniol, limonene, cumic alcohol, etc., and the fruit contains sterol, unsaturated organic acid, etc. The other materials can be propolis, radix Puerariae, folium Artemisiae Argyi, herba Taraxaci, etc.

Supercritical carbon dioxide fluid extraction (SFE) is performed by utilizing the relationship between the dissolving capacity and density of a supercritical fluid, i.e., by utilizing the influence of pressure and temperature on the dissolving capacity of a supercritical fluid. The supercritical extraction is easy to operate, the temperature is low, and the heat-sensitive substances, the extracts and the raffinate thereof are protected from being polluted by organic solvents. Under the supercritical state, the carbon dioxide has strong penetrating power to solid substances and strong extraction performance to aromatic compounds, so the supercritical extraction technology has wide application in the extraction of effective components of natural products and essences and spices. Drying the two substances at 50 ℃ to ensure that the water content is less than 7%, crushing the two substances into coarse powder by a crusher, and performing carbon dioxide fluid extraction, wherein the apparatus is produced by Jiangsu Nantong Huaan supercritical extraction limited company, the entrainer is ethanol with the content of 3.5%, the extraction pressure is 20MPa, the extraction temperature is 30 ℃, the separation pressure is 8MPa, and the separation temperature is 55 ℃, so that an extraction product is obtained. Keeping the temperature of the extraction product at 5 ℃ for 100min, centrifuging at 6000r/min for 20min to remove the impurities precipitated at the bottom, and vacuumizing the supernatant in a vacuum box at 45 ℃ for 8h to obtain the clove pepper extract.

The supercritical carbon dioxide fluid extraction and the impregnation method can be adopted, the required equipment is simple and easy to operate, the supercritical carbon dioxide fluid extraction and the impregnation method are used for crushing the supercritical carbon dioxide fluid and the impregnation method, the solvent is added for impregnation, and the stirring is carried out constantly, generally four to six days are needed, but the heating is not needed, the energy consumption is saved, and the filtration is carried out, so that the extract is obtained.

The extraction method can also adopt percolation method, which is a method of placing moderately pulverized medicinal materials in a percolation cylinder, continuously adding solvent from the upper part, and leaching out medicinal materials in the process of flowing downwards when the solvent permeates through the medicinal material layer. The method does not require heating, and the percolate is the extract.

The extraction method can also adopt steam distillation method, which is extraction method comprising co-distilling plant material containing volatile components with water to distill volatile components with steam, and condensing to collect volatile components.

The supercritical extract liquid or the impregnation liquid or the percolation liquid obtained by the method has dark color and brownish black color, and if the supercritical extract liquid or the impregnation liquid or the percolation liquid is directly sprayed on insoles and socks, the insoles, the socks and the shoes are easily polluted, and the acceptance degree in use is not high, so that a good decoloring method is necessary to remove pigments and retain effective components.

The decolorization method can be alumina column chromatography. The alumina adsorbent is white spherical porous particle with homogeneous granularity, smooth surface, high mechanical strength, no swelling, no cracking and no toxicity, no smell, no bad smell, no water and organic solvent, and is prepared with high purity alumina through scientific compounding, catalytic fine machining, and may be used in decolorizing, etc.

The decolorizing method can also adopt activated carbon column chromatography. The activated carbon has a developed pore structure, a large specific surface area and abundant surface chemical groups, and has strong specific adsorption capacity and good decolorization effect.

The decolorizing method can also adopt silica gel column chromatography. Silica gel used for column chromatography can be divided into normal phase silica gel and reverse phase silica gel, and normal phase silica gel column chromatography packing is normal phase silica gel, which is suitable for separating compounds with relatively small polarity, so that organic solvents with relatively small polarity are usually selected as eluents.

The three methods can well remove pigment and retain effective components.

The application method of the deodorant solution comprises spraying the deodorant solution onto the front and back surfaces of the insole at night, 4-7mL per insole, standing at room temperature for one night, placing the insole into a shoe for use the next day, spraying 1mL per sock, 0.5mL per left foot and right foot, changing and washing the socks once a day, changing and washing the insole once a day for three days, and 2 weeks is a treatment course. For the standard of curative effect, Zhao Xiu Hua and Zhang Wanshan preparation and clinical application of beriberi and smelly foot cleaner (Zhang Jia Kou Yi Xue Xuan, 2004 (24): 34-35): the desquamation and the small blister completely disappear, no itch exists, no relapse occurs, and the foot odor disappears, so that the treatment is realized; the scurf and the small blister basically disappear, the itching does not exist, and the foot odor disappears, so the effect is obvious; the scurf removal and small blister are reduced, the pruritus is relieved, the subjective symptoms are improved, the foot odor is not obvious, and the effect is achieved; no effect is obtained.

The deodorant liquid is sprayed on the insole for treating beriberi and foot odor, and the insole can be placed in the shoe for a long time to exert the functions of deodorization and bacteriostasis for a long time. The deodorant has good treatment effect on foot odor, the effective rate reaches 100%, and the deodorant is simple, convenient, easy, nontoxic, safe and reliable in treatment effect. The used materials are clove and pepper which are edible materials, have no toxic or side effect, are all natural substances and are easy to accept by users.

Drawings

FIG. 1 HPLC chromatogram of eugenol control.

FIG. 2 HPLC chromatogram of deodorant solution (method 1).

FIG. 3 HPLC chromatogram of deodorant solution (2 method).

FIG. 4 HPLC chromatogram of deodorant solution (3 method).

FIG. 5 HPLC chromatogram of deodorant solution (4 method).

[ detailed description ] embodiments

Example (b): 1

Taking 900 g of clove and 100 g of pepper, drying until the water content is 7 wt%, and then crushing to 50 meshes to obtain the powder of the clove and the pepper; adding flos Caryophylli powder into carbon dioxide supercritical extraction device (supercritical extraction of Huaan from south China, Jiangsu, 3.5%), extracting with ethanol (3.5%) under 20MPa at 30 deg.C and 8MPa at 55 deg.C to obtain extract. Keeping the temperature of the extraction product at 5 ℃ for 100min, centrifuging at 6000r/min for 20min to remove the impurities precipitated at the bottom, and vacuumizing the supernatant in a vacuum box at 45 ℃ for 8h to obtain the clove pepper extract. Dissolving the clove pepper extract to 2L by using propylene glycol, adding the clove pepper extract into a chromatographic column filled with 1KG alumina, passing through the column, decoloring to obtain decolored liquid, and washing the column by using a proper amount of propylene glycol to obtain 2L of decolored liquid, namely deodorizing liquid (method 1).

Example (b): 2

The content determination method of the deodorant liquid (1 method) is as follows:

measuring by high performance liquid chromatography.

Octadecylsilane chemically bonded silica is used as a filler in chromatographic conditions and system applicability tests; acetonitrile is taken as a mobile phase A, water is taken as a mobile phase B, and isocratic elution is carried out at A: B of 45: 55; the detection wavelength was 215 nm. The number of theoretical plates should not be less than 3000 calculated according to eugenol peak.

Preparation of control solution eugenol control is precisely weighed and added with methanol to prepare mixed solution containing 0.48mg per 1ml, and the mixed solution is shaken up to obtain the eugenol control.

Preparing a test solution, namely putting 1mL of deodorant bacteriostatic solution into a 100mL measuring flask, adding propylene glycol to 100mL, and shaking up to obtain the test solution.

The determination method comprises precisely sucking 20 μ L of reference solution and 20 μ L of test solution, injecting into liquid chromatograph, and determining.

HPLC chromatogram of eugenol reference substance is shown in figure 1: 0.48 mg/mL. Eugenol peak area A is 28089046

The HPLC chromatogram of the deodorant solution (1 method) is shown in figure 2: diluting 100 times, and the eugenol peak area A is 18437456.

The deodorant liquid (1 method) contains eugenol as follows: 18437456/28089046 × 0.48mg/mL × 100 × 31.51 mg/mL.

Example (b): 3

Spraying deodorant solution (1 method) on the front and back surfaces of insole at night, 5mL per insole, standing at room temperature for one night, placing the insole in shoes for use the next day, spraying 1mL on each sock, 0.5mL on left and right feet, changing and washing socks once a day, changing and washing the insole once every three days, and 2 weeks is a treatment course. The curative effect standard is as follows: the desquamation and the small blister completely disappear, no itch exists, no relapse occurs, and the foot odor disappears, so that the treatment is realized; the scurf and the small blister basically disappear, the itching does not exist, and the foot odor disappears, so the effect is obvious; the scurf removal and small blister are reduced, the pruritus is relieved, the subjective symptoms are improved, the foot odor is not obvious, and the effect is achieved; no effect is obtained. (Zhao Xiu Hua, Zhang Wan shan Jia Qi Jing, Jia Kou Xue Jing, 2004 (24): 34-35.)

The treatment groups were 37, 29 men and 8 women.

The total cases of the deodorant liquid (1 method) group are 37 cases, 27 cases are cured, 6 cases are obviously effective, 4 cases are effective, 0 case is ineffective, and the total effective rate is 100%.

The deodorant liquid is used for treating beriberi and foot odor, and is simple, convenient, feasible, nontoxic, safe and reliable in curative effect.

Example (b): 4

Taking 180 g of clove and 820 g of pepper, and crushing to 40 meshes to obtain the powder of the clove and the pepper; adding flos Caryophylli powder into isopropanol 3L, soaking at room temperature for four days, and filtering to obtain filtrate 1.9L. Adding the filtrate into a chromatographic column filled with 0.5KG of active carbon (dry column packing), passing through the column, decolorizing to obtain decolorized solution, washing the column with appropriate amount of isopropanol, adding into the previous decolorized solution to obtain 2L of decolorized solution, and shaking up to obtain deodorizing solution (2 method).

Example (b): 5

The content determination method of the deodorization bacteriostat liquid (2 method) is as follows:

measuring by high performance liquid chromatography.

Preparing a test solution, namely putting 1mL of deodorant bacteriostatic solution into a 100mL measuring flask, adding isopropanol to 100mL, and shaking up to obtain the test solution.

The concentration of the eugenol control product is 0.48mg/mL, and the peak area A is 28089046.

The HPLC chromatogram of the deodorant solution (2 method) is shown in figure 3: adding 1mL of deodorant solution (2 method) into a 20mL measuring flask, adding isopropanol scale, and shaking up to dilute 20 times. Eugenol peak area a is 9218220.

The deodorant liquid (2 method) contains eugenol as follows: 9218220/28089046 × 0.48mg/mL × 100 ═ 15.75 mg/mL.

Example (b): 6

Spraying deodorant solution (2 method) on the front and back surfaces of insole at night, placing 7mL of each insole at room temperature for one night, placing the insole in shoes for use the next day, spraying 1mL of deodorant solution on each sock, spraying 0.5mL of deodorant solution on the left and right feet, changing and washing the socks once every day, changing and washing the insole once every three days, and 2 weeks is a treatment course. The curative effect standard is as follows: the desquamation and the small blister completely disappear, no itch exists, no relapse occurs, and the foot odor disappears, so that the treatment is realized; the scurf and the small blister basically disappear, the itching does not exist, and the foot odor disappears, so the effect is obvious; the scurf removal and small blister are reduced, the pruritus is relieved, the subjective symptoms are improved, the foot odor is not obvious, and the effect is achieved; no effect is obtained. (Zhao Xiu Hua, Zhang Wan shan Jia Qi Jing, Jia Kou Xue Jing, 2004 (24): 34-35.)

Treatment groups were 43, 33 men and 10 women.

The total cases of the deodorant liquid (2 methods) group are 43 cases, 33 cases are cured, 6 cases are obviously effective, 4 cases are effective, 0 case is ineffective, and the total effective rate is 100%.

The deodorant solution can be sprayed on insole and socks for treating tinea pedis and foot odor, and has no adverse reaction such as burn, pruritus, red swelling, burning sensation, and pain.

Example (b): 7

990 g of clove and 10 g of pepper are taken and crushed to 50 meshes to obtain the powder of the clove and pepper; adding flos Caryophylli powder into 50% ethanol 1.2L, stirring at room temperature for 24 hr to fully absorb solvent, filling the flos Caryophylli powder fully absorbing solvent into percolation barrel, percolating with 50% ethanol at percolation speed of 0.5-0.6 mL/min, and collecting percolate 2L. Adding the filtrate into chromatographic column containing 0.4KG silica gel (dry column packing), passing through the column, decolorizing to obtain decolorized solution, washing the column with appropriate amount of 50% ethanol, mixing with the previous decolorized solution to obtain 2L decolorized solution, and shaking to obtain deodorizing solution (3 method).

Example (b): 8

The content determination method of the deodorization bacteriostat liquid (3 method) is as follows:

measuring by high performance liquid chromatography.

Preparing a test solution, namely putting 1mL of deodorant bacteriostatic solution into a 100mL measuring flask, adding 50% ethanol to 100mL, and shaking up to obtain the test solution.

The HPLC chromatogram of the deodorant solution (method 3) is as follows: taking 1mL of deodorant solution (3 method) to a 100mL measuring flask, adding 50% ethanol to the scale, shaking up, and diluting by 10 times. Eugenol peak area a is 35776598.

The HPLC chromatogram of the deodorant solution (3 method) is shown in figure 4.

The deodorant liquid (3 method) contains eugenol as follows: 35776598/28089046 × 0.48mg/mL × 100 ═ 61.14 mg/mL.

Example (b): 9

Spraying deodorant solution (3 method) on the front and back surfaces of insole at night, 4mL per insole, standing at room temperature for one night, placing the insole in shoes for use the next day, spraying 1mL on each sock, 0.5mL on left and right feet, changing and washing socks once a day, changing and washing the insole once every three days, and 2 weeks is a treatment course. The curative effect standard is as follows: the desquamation and the small blister completely disappear, no itch exists, no relapse occurs, and the foot odor disappears, so that the treatment is realized; the scurf and the small blister basically disappear, the itching does not exist, and the foot odor disappears, so the effect is obvious; the scurf removal and small blister are reduced, the pruritus is relieved, the subjective symptoms are improved, the foot odor is not obvious, and the effect is achieved; no effect is obtained. (Zhao Xiu Hua, Zhang Wan shan Jia Qi Jing, Jia Kou Xue Jing, 2004 (24): 34-35.)

Treatment groups were 39, 33 men and 6 women.

The total cases of the deodorant liquid (3 methods) group are 39 cases, 32 cases are cured, 4 cases are obviously effective, 3 cases are effective, 0 case is ineffective, and the total effective rate is 100%.

The deodorizing liquid is sprayed on the insole for treating beriberi and foot odor, the used materials are clove and pepper which are edible materials, the deodorizing liquid has no toxic or side effect, and is all natural substances, so that the deodorizing liquid is easy to accept by a user.

Example (b): 10

Taking 900 g of clove and 100 g of pepper, and crushing to 40 meshes to obtain the powder of the clove and the pepper; placing the ground clove bud powder into a distillation flask, adding 2.5L of water, soaking for 1 hour, connecting a condenser tube, starting distillation, distilling with soft fire for 3 hours, collecting an oil-water mixture, transferring the oil-water mixture into a separating funnel, standing for one night, and separating and taking an oil layer after the oil-water mixture is layered. Adding Tween-8010 mL into flos Caryophylli and fructus Zanthoxyli, stirring, adding 70% 7 alcohol to 2L, and shaking to obtain deodorant solution (method 4).

Example (b): 11

The content determination method of the deodorizing bacteriostat (4 method) is as follows:

measuring by high performance liquid chromatography.

Preparing a test solution, namely putting 1mL of deodorant bacteriostatic solution into a 100mL measuring flask, adding methanol to 100mL, and shaking up to obtain the test solution.

The HPLC chromatogram of the deodorant solution (4 method) is as follows: taking 1mL of deodorant solution (4 method) to a 100mL measuring flask, adding 70% ethanol to the scale, shaking up, and diluting by 10 times. Eugenol peak area a is 35010944.

HPLC chromatogram of deodorant solution (4 method) is shown in figure 5.

The deodorizing liquid (4 method) contains eugenol as follows: 35010944/28089046 × 0.48mg/mL × 100 ═ 59.83 mg/mL.

Example (b): 12

Spraying deodorant solution (4 method) on the front and back surfaces of insole at night, 4mL per insole, standing at room temperature for one night, placing the insole in shoes for use the next day, spraying 1mL on each sock, 0.5mL on left and right feet, changing socks once every day, changing insole once every three days, and 2 weeks as a treatment course. The curative effect standard is as follows: the desquamation and the small blister completely disappear, no itch exists, no relapse occurs, and the foot odor disappears, so that the treatment is realized; the scurf and the small blister basically disappear, the itching does not exist, and the foot odor disappears, so the effect is obvious; the scurf removal and small blister are reduced, the pruritus is relieved, the subjective symptoms are improved, the foot odor is not obvious, and the effect is achieved; no effect is obtained. (Zhao Xiu Hua, Zhang Wan shan Jia Qi Jing, Jia Kou Xue Jing, 2004 (24): 34-35.)

The treatment groups were 29, 25 men and 4 women.

The total cases of the deodorant liquid (4 methods) group are 29 cases, 22 cases are cured, 4 cases are obviously effective, 3 cases are effective, 0 case is ineffective, and the total effective rate is 100%.

The deodorant liquid is sprayed on the insole for treating beriberi and foot odor, and the insole can be placed in the shoe for a long time to exert the functions of deodorization and bacteriostasis for a long time.

Example (b): 13

Trichophyton rubrum (Trichophyton rubrum) is one of the main pathogenic bacteria of dermatophytosis, and the bacteriostatic action of the deodorant liquid on the Trichophyton rubrum is investigated. The experimental lyophilized strain of Trichophyton rubrum is purchased from Shanghai Huseis Biotechnology Limited and refrigerated in a refrigerator at 4 ℃ to be used as the strain to be detected. Preparation of bacterial suspension: inoculating the tested strain trichophyton rubrum on a Saburgh Laoguo glucose agar culture medium, carrying out continuous passage for 2 times, taking a single bacterial colony with the bacterial colony diameter larger than 1mm, dissolving the single bacterial colony in 10mL of sample diluent, and adjusting the turbidity of the bacterial suspension to (1-5 multiplied by 10) by using a turbidity analyzer⁶CFU/mL, diluting the bacterial suspension by 100 times with a liquid culture medium, wherein the concentration of the bacterial liquid is 1-5 multiplied by 10⁴CFU/mL. Preparation of drug sensitive test plate: taking a 96-well culture plate, adding the deodorization solution into the culture plate, wherein the maximum volume of each well is 4mL, adding 1mL of deodorization solution and 1mL of liquid broth culture medium into No. 1 well of No. 1 well, uniformly mixing, taking 1mL of No. 1 well by using a pipette, adding into No. 2 well, adding 1mL of liquid broth culture medium, uniformly mixing, and continuously diluting by 2 times by using the liquid broth culture medium by analogy, thereby preparing ten liquid medicines with different concentrations of 1: 1, 1: 2, 1: 4, 1: 8, 1: 16, 1: 32, 1: 64, 1: 128, 1: 256 and 1: 512, which respectively contain 0.5mL/mL, 0.25mL/mL, 0.125mL/mL, 0.0625mL/mL, 0.0312mL/mL, 0.0156mL/mL, 0.0078mL/mL, 0.0039mL, 0.0019mL/mL, and 0.00098mL of deodorization solution. Inoculation of the bacterial suspension: taking out the prepared bacterial suspension, and sequentially adding 50uL of trichophyton rubrum bacterial liquid into 12 holes according to the arrangement of the cell culture plates, wherein the concentration of the bacterial liquid is 1-5 multiplied by 10 respectively⁴CFU/mL. Determination of Minimum Inhibitory Concentration (MIC): covering two groups of prepared drug sensitive flat plates, placing the drug sensitive flat plates in a constant temperature box at 28 ℃ for culturing, taking out the drug sensitive flat plates after 3-5 days to observe the growth condition of the tested strains on the cell culture plate, wherein if cotton floc appears in culture holes, the growth of fungi is shown, and the liquid medicine has no bacteriostatic action; if the liquid in the culture hole is clear, no fungus grows, and the liquid medicine has the bacteriostatic action. The negative sign indicates the growth of a completely sterile colony, the positive sign indicates that the diameter of the growing colony is less than 0.5cm, the positive sign indicates that the diameter of the growing bacterium block is between 0.5 and 1.0cm, the positive sign indicates that the diameter of the growing bacterium block is more than 1cm, and the lowest drug concentration of the deodorant solution test tube without the growth of fungi is used as the most deodorant solution for the seed bacteriumLow inhibitory concentration (MIC) value.

Therefore, the four deodorant liquids have good bacteriostatic action on trichophyton rubrum. When the deodorant solution (method 1) and the deodorant solution (method 2) are diluted to 1/8 of the original concentration, good bacteriostatic action still exists, and when the deodorant solution (method 3) and the deodorant solution (method 4) are diluted to 1/16 of the original concentration, good bacteriostatic action still exists.

The above embodiments are merely exemplary embodiments of the present invention, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art, and the contents of the changes still fall within the scope of the present invention.

Claims

1. A deodorant liquid for preventing and treating beriberi and foot odor is characterized in that: flos Caryophylli and fructus Zanthoxyli are extracted by proper extraction method, and decolorized by proper decolorization method to remove pigment, and the deodorant solution for preventing and treating loempe and foot odor is prepared.

2. The deodorant liquid according to claim 1, wherein: the flos Caryophylli and fructus Zanthoxyli can be extracted by supercritical extraction, soaking, percolation, and steam distillation.

3. The deodorant liquid according to claim 1, wherein: the solvent used in the extraction process can be carbon dioxide supercritical fluid, isopropanol, ethanol, and water.

4. The deodorant liquid according to claim 1, wherein: the clove and the pepper are deep in color after being extracted, and need to be decolored by adopting a proper decoloring method so as to avoid polluting insoles and socks.

5. The deodorant liquid according to claim 1, wherein: the decoloring method may be an alumina method, an activated carbon method, a silica gel method, or the like.

6. The deodorant liquid according to claim 1, wherein: the deodorant solution is sprayed on insole, and the insole can be placed in shoes for use, or directly sprayed on socks and feet.

7. The deodorant liquid according to claim 1, wherein: the content of eugenol is 10mg/mL-100 mg/mL.

8. The deodorant liquid according to claim 1, wherein: the ratio of clove to pepper is 99: 1 to 18: 82.