CN112798346B - Experiment method and system for gas-liquid co-sampling in biogas fermentation batch experiment - Google Patents
Experiment method and system for gas-liquid co-sampling in biogas fermentation batch experiment Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 115
- 230000004151 fermentation Effects 0.000 title claims abstract description 110
- 238000005070 sampling Methods 0.000 title claims abstract description 89
- 239000007788 liquid Substances 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000002474 experimental method Methods 0.000 title claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 238000012360 testing method Methods 0.000 claims abstract description 22
- 238000007599 discharging Methods 0.000 claims abstract description 8
- 230000000149 penetrating effect Effects 0.000 claims abstract description 3
- 238000001514 detection method Methods 0.000 claims description 30
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 19
- 239000000741 silica gel Substances 0.000 claims description 19
- 229910002027 silica gel Inorganic materials 0.000 claims description 19
- 230000005540 biological transmission Effects 0.000 claims description 17
- 238000004458 analytical method Methods 0.000 claims description 16
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 12
- 230000004927 fusion Effects 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 5
- 238000001125 extrusion Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 230000002452 interceptive effect Effects 0.000 claims description 3
- 230000008054 signal transmission Effects 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 238000013461 design Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000012544 monitoring process Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 241001411320 Eriogonum inflatum Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D21/00—Measuring or testing not otherwise provided for
- G01D21/02—Measuring two or more variables by means not covered by a single other subclass
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2226—Sampling from a closed space, e.g. food package, head space
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N2001/1031—Sampling from special places
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1418—Depression, aspiration
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The invention belongs to the technical field of biogas fermentation test, and discloses an experimental method and an experimental system for gas-liquid co-sampling in a biogas fermentation batch test, wherein a water collecting bottle is connected with a gas collecting and water discharging bottle through a first conduit, and a first rubber plug is plugged at the upper side of the gas collecting and water discharging bottle; the gas-collecting drainage bottle is connected with the balloon through a second conduit, and the balloon is connected with the fermentation bottle through an exhaust conduit; the upper side of the fermentation bottle is plugged with a second rubber plug, two through holes are formed in the middle of the second rubber plug, and a sampling conduit and an exhaust conduit are respectively arranged in the two through holes in a penetrating mode. The invention solves the problems of air leakage, uneven gas sampling and difficult liquid sampling of the fermentation system, and can sample both gas and liquid through the sampling port pipe, thereby not only simplifying the sampling, but also ensuring the reliability of the sampled product. In the whole liquid and gas taking process, reliable gas samples and liquid samples can be obtained without opening a fermentation bottle, so that the stability of a fermentation system is greatly improved, and meanwhile, the method is convenient and concise, and the cost is greatly saved.
Description
Technical Field
The invention belongs to the technical field of biogas fermentation testing, and particularly relates to an experimental method and an experimental system for gas-liquid co-sampling in a biogas fermentation batch test.
Background
At present, the full-automatic methane potential testing system of the device for fermenting the marsh gas batch in the market, such as the full-automatic methane potential testing system of the Swedish Bipu, has high degree of automation, can monitor the change of the gas content, but has low detection accuracy of the gas content, and high selling price, the market selling price of a set of equipment is about 15-20 ten thousand, and in addition, the system can not monitor the change of relevant parameters of fermentation liquid such as PH, VFAs and the like in the fermentation process. The conventional experimental device for anaerobic fermentation of the batch biogas is mainly a water and gas collecting method, and the water and gas collecting method is mainly divided into a direct gas collecting method and an indirect gas collecting method, namely a triple gas collecting and water discharging method.
The direct gas collecting method is to extrude the water in the graduated cylinder which is inverted in the water tank out of the collector through the exhaust pipe. Although the gas yield of the reaction apparatus can be visually seen, it is very inconvenient to equip each apparatus with a water tank and fix the measuring cylinder filled with water to the water tank. Since batch fermentations typically have many reactors, such devices are very inconvenient to operate in practice. The most common method at the present stage is a triple gas collection drainage method.
The triple gas collection drainage method is connected in series by three bottles as shown in fig. 6. Wherein A is a fermentation reaction bottle, B is gas collection and water drainage, and C is a water collection bottle. And (3) carrying out anaerobic fermentation on the bottle A to generate biogas. As the air pressure in the a bottle and the tubing connected to the B bottle increases, the water in B is squeezed into the C bottle, and the gas production in a is measured indirectly by measuring the volume of liquid in C. But the triple gas collecting and draining device has three problems, firstly, the device leaks air. Second, the gas distribution within the system is non-uniform, resulting in non-representative gas samples being obtained. Thirdly, obtaining a fermentation broth sample is difficult under the condition that the fermentation system gas is not disturbed.
There are various methods for dealing with the air leakage problem, such as using rubber plugs, replacing reaction bottles with better tightness, silica gel gaskets, etc., so that the possibility of air leakage is greatly reduced to a certain extent, but the time and the labor are wasted. Because the sampling conduit is long and narrow, the gas flowing into the sampling conduit is poor, the gas sample directly obtained from the sampling conduit cannot well represent the gas condition inside the fermentation system, and the representativeness of the obtained gas sample is ensured by shortening the length of the sampling conduit or by repeatedly pumping the sampling needle back and forth, but the consistency of the obtained gas sample and the gas environment of the fermentation system is still difficult to ensure. Currently, laboratory methods for taking liquid samples from fermentation flasks are generally both open sampling and syringe sampling. Because anaerobic fermentation is a relatively anaerobic fermentation device, the open sampling method of fermentation liquid in the anaerobic fermentation device not only can enable the device to enter air, but also can influence the gas environment of the fermentation device, and further, inconvenience is brought to gas component monitoring. In the syringe sampling method, since the syringe needle is thin, it is difficult to extract a liquid sample from a fermentation liquid containing a large amount of impurities.
Through the above analysis, the problems and defects existing in the prior art are as follows:
(1) The existing automatic fermentation device is very expensive, and the change of physicochemical properties of fermentation liquid in the fermentation process cannot be monitored.
(2) The existing sealing mode greatly reduces the possibility of air leakage to a certain extent, but is time-consuming and labor-consuming.
(3) The open sampling method not only can enable the device to enter air, but also can influence the gas environment of the fermentation device, and is more inconvenient for monitoring the gas components.
(4) The bottle stopper is easy to leak by sampling the syringe and inserting the needle for many times, and in addition, the liquid sample is difficult to extract from the fermentation liquid with many impurities because the syringe needle is thin.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides an experimental method and an experimental system for gas-liquid co-sampling in a biogas fermentation batch experiment.
The invention is realized in such a way that an experiment system for gas-liquid co-sampling in a biogas fermentation batch experiment is provided with a water collecting bottle;
the water collecting bottle is connected with the gas collecting and water discharging bottle through a first conduit, and a first rubber plug is plugged at the upper side of the gas collecting and water discharging bottle;
the gas-collecting and water-draining bottle is connected with a balloon through a second conduit, and the balloon is connected with the fermentation bottle through a gas-draining conduit;
the upper side of the fermentation bottle is plugged with a second rubber plug, two through holes are formed in the middle of the second rubber plug, and a sampling conduit and an exhaust conduit are respectively arranged in the two through holes in a penetrating manner;
the fermentation bottle is filled with fermentation substrate and inoculum, the gas-collecting and water-draining bottle is filled with water, and the gas-collecting bottle is an open empty bottle.
Further, the sampling catheter is connected with the exhaust catheter through an air duct, and a second switch is arranged in the middle of the air duct.
Further, the first rubber plug and the second rubber plug are fixed with data wires in a sealing mode through the through holes, and the data wires are flat data wires and can be tightly attached to the data wires through the rubber plug holes.
Furthermore, the outer end plug of the sampling catheter is provided with an ear-shaped silica gel plug, the outer part of the ear-shaped silica gel plug is provided with an overhanging annular ear, the middle of the ear-shaped silica gel plug is provided with two jacks for inserting glass tubes, and a table-shaped plug body is arranged below the ear-shaped silica gel plug.
Further, an air pressure sensor is fixed at the lower end of the second rubber plug.
Further, the fermentation bottle is inside to be provided with temperature sensor and pH sensor, temperature sensor and pH sensor set up in the sampling pipe lower extreme outside.
Further, the experimental system for gas-liquid co-sampling in the biogas fermentation batch experiment further comprises:
the temperature detection module is connected with the central control module and detects the temperature inside the fermentation bottle through a temperature sensor;
the pH detection module is connected with the central control module and is used for detecting the pH value in the fermentation bottle through the pH sensor;
the air pressure detection module is connected with the central control module and detects the air pressure inside the fermentation bottle through the air pressure sensor;
the central control module is respectively connected with the temperature detection module, the gas detection module, the air pressure detection module, the data transmission module, the fermentation temperature control module, the data comprehensive analysis module, the alarm module and the display module, and coordinates the normal operation of each module.
Further, the data transmission module is used for interactive transmission of data through signal transmission equipment;
the cloud service module is connected with the data transmission module and processes the whole system data by utilizing a big data technology through a cloud server;
the fermentation temperature control module is used for changing the temperature of the fermentation bottle through a temperature regulator; the data comprehensive analysis module processes the whole system data through a data processing program;
the alarm module is used for alarming through alarm equipment; the display module is used for displaying corresponding data through the display screen.
Further, the data comprehensive analysis module processes the whole system data through a data processing program, and the specific process is as follows:
establishing a data set by using data corresponding to a temperature detection module, a gas pressure detection module, a data transmission module, a fermentation temperature control module, a data comprehensive analysis module, an alarm module and a display module;
classifying the data in the data set, and extracting corresponding target features;
and obtaining the characteristic quantity of the fusion target through a fusion algorithm, and carrying out target classification and identification.
Another object of the present invention is to provide an experimental method for gas-liquid co-sampling in a biogas fermentation batch test, which includes:
step one, when fermentation is carried out, a sampling conduit is closed, methane is generated in a fermentation bottle, so that the internal air pressure of a system is increased, the gas enters a gas collecting and draining bottle along the conduit, and water in the gas collecting and draining bottle is drained into an open empty bottle;
when liquid samples are taken, the first switch is closed, the communication of gas in the sampling catheter and the exhaust catheter is blocked, the second switch is closed, the gas guide tube is blocked, the sampling catheter is opened, and the liquid in the system is discharged by extruding the balloon;
step three, as the sampling port guide pipe stretches into the liquid level, the liquid in the guide pipe plays a role of liquid seal, and external gas can be effectively prevented from entering the system;
step four, extruding the balloon to increase the air pressure of the fermentation bottle, and stably flowing out the liquid along the sampling conduit from the sampling conduit;
and fifthly, when the gas sample is taken, closing the sampling conduit, closing the second switch, opening the first switch to enable the gas in the conduit to be communicated, at the moment, communicating the sampling conduit with the exhaust conduit, closing the second switch, releasing the balloon through continuous extrusion, enabling the gas in the fermentation system to be fully and uniformly mixed, and taking the gas sample from the sampling conduit by using the sampling needle.
By combining all the technical schemes, the invention has the advantages and positive effects that:
the invention solves the problems of air leakage, uneven gas sampling and difficult liquid sampling of the fermentation system, innovatively designs the sampling conduit, and the sampling conduit can not only sample gas but also liquid, thereby not only ensuring the sampling to be simpler, but also ensuring the reliability of the sampled product. In the whole liquid and gas taking process, reliable gas samples and liquid samples can be obtained without opening a fermentation bottle, and the stability of a fermentation system is greatly improved. Through automatic pressure sensor and temperature sensor, the inside fermentation condition of real-time supervision fermentation system has made things convenient for fermentation system stability monitoring and stability prediction, and this design is convenient succinct simultaneously, has greatly saved the cost of batch fermentation.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an experimental system for gas-liquid co-sampling in a biogas fermentation batch experiment according to an embodiment of the invention.
Fig. 2 is a schematic structural diagram of a fermentation bottle according to an embodiment of the present invention.
Fig. 3 is a schematic structural view of an ear-type silica gel plug according to an embodiment of the present invention.
Fig. 4 is a flowchart of an experimental method for gas-liquid co-sampling in a biogas fermentation batch test provided by an embodiment of the invention.
Fig. 5 is a schematic diagram of a control system structure in an experimental system for gas-liquid co-sampling in a biogas fermentation batch test according to an embodiment of the invention.
Fig. 6 is a schematic structural diagram of a triple gas-collecting and water-draining device in the prior art according to an embodiment of the present invention.
In the figure: 1. a water collecting bottle; 2. a first conduit; 3. a second conduit; 4. a first rubber stopper; 5. air collecting and draining bottle; 6. a balloon; 7. a sampling catheter; 8. a fermentation bottle; 9. a first switch; 10. an exhaust duct; 11. an air duct; 12. a second switch; 13. an air pressure sensor; 14. a temperature sensor, a pH sensor; 15. a second rubber stopper; 16. ear-shaped silica gel plug; 17. a temperature detection module; 18. a pH detection module; 19. an air pressure detection module; 20. a display module; 21. a central control module; 22. a data transmission module; 23. a cloud service module; 24. a fermentation temperature control module; 25. a data comprehensive analysis module; 26. and an alarm module.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Aiming at the problems in the prior art, the invention provides an experimental method and an experimental system for gas-liquid co-sampling in a biogas fermentation batch test, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1 to 2, a water collecting bottle 1 in an experiment system for gas-liquid co-sampling in a biogas fermentation batch experiment provided by the embodiment of the invention is connected with a gas collecting and draining bottle 5 through a first conduit 2, and a first rubber plug 4 is plugged at the upper side of the gas collecting and draining bottle 5; the gas collecting and draining bottle 5 is connected with a balloon 6 through a second conduit 3, and the balloon 6 is connected with a fermentation bottle 8 through a draining conduit 10; the upper side of the fermentation bottle 8 is plugged with a second rubber plug 15, two through holes are formed in the middle of the second rubber plug 15, and a sampling conduit 7 and an exhaust conduit 10 are respectively penetrated in the two through holes; during fermentation, fermentation substrate and inoculum are added to the fermentation flask 8 to perform biogas fermentation. The gas collecting and draining bottle is filled with water, and the gas collecting bottle is an open empty bottle. The sampling conduit 7 is connected with the exhaust conduit 10 through an air duct 11, and a second switch 12 is arranged in the middle of the air duct 11. The first rubber stopper 4 and the second rubber stopper 15 are hermetically fixed with data lines through the through holes. The lower end of the second rubber plug 15 is fixed with an air pressure sensor 13. The fermentation bottle 8 is internally provided with a temperature sensor and a pH sensor 14, and the temperature sensor and the pH sensor are arranged outside the lower end of the sampling conduit 7.
As shown in fig. 3, the outer end of the sampling catheter 7 is plugged with an ear-shaped silica gel plug 16, the outside of the ear-shaped silica gel plug 16 is provided with an overhanging annular ear, the middle of the ear-shaped silica gel plug is provided with two insertion holes for inserting glass tubes, and a table-shaped plug body is arranged below the ear-shaped silica gel plug. The ear-shaped silica gel plug has double guarantee for letting the device have better airtight design, first, the outside annular ear that has overhanging of ear-shaped silica gel plug, annular ear be equivalent to with the gas leakage prevention gasket, compress tightly through the bottle plug, greatly got rid of the gas leakage possibility. Second, this design adopts the silica gel material, utilizes the good characteristic of silica gel pliability, and the cock body size slightly is greater than the bottleneck, and jack size slightly is less than glass tube diameter. After the glass tube is inserted, the bench type plug body is tightly attached to the bottle mouth through extrusion, so that the air tightness of the system is further improved.
As shown in fig. 4, the experimental system for gas-liquid co-sampling in the biogas fermentation batch test provided by the embodiment of the invention further includes:
the temperature detection module 17 is connected with the central control module and detects the temperature inside the fermentation bottle through a temperature sensor;
the pH detection module 18 is connected with the central control module and is used for detecting the pH value in the fermentation bottle through a pH sensor;
the air pressure detection module 19 is connected with the central control module and detects the air pressure inside the fermentation bottle through an air pressure sensor;
the central control module 21 is respectively connected with the temperature detection module, the gas detection module, the air pressure detection module, the data transmission module, the fermentation temperature control module, the data comprehensive analysis module, the alarm module and the display module, and coordinates the normal operation of each module.
The data transmission module 22 is used for the interactive transmission of data through signal transmission equipment;
the cloud service module 23 is connected with the data transmission module and processes the whole system data by utilizing a big data technology through a cloud server;
a fermentation temperature control module 24 for changing the temperature of the fermentation bottle by a temperature regulator; the data comprehensive analysis module processes the whole system data through a data processing program;
an alarm module 25 for alarming by an alarm device; the display module is used for displaying corresponding data through the display screen.
Further, the data comprehensive analysis module processes the whole system data through a data processing program, and the specific process is as follows:
establishing a data set by using data corresponding to a temperature detection module, a gas pressure detection module, a data transmission module, a fermentation temperature control module, a data comprehensive analysis module, an alarm module and a display module;
classifying the data in the data set, and extracting corresponding target features;
and obtaining the characteristic quantity of the fusion target through a fusion algorithm, and carrying out target classification and identification.
The data comprehensive analysis module provided by the embodiment of the invention processes the whole system data through a data processing program, wherein the specific process comprises the following steps:
establishing a data set by using data corresponding to a temperature detection module, a gas pressure detection module, a data transmission module, a fermentation temperature control module, a data comprehensive analysis module, an alarm module and a display module;
classifying the data in the data set, and extracting corresponding target features;
and obtaining the characteristic quantity of the fusion target through a fusion algorithm, and carrying out target classification and identification.
As shown in fig. 5, the experimental method for gas-liquid co-sampling in the biogas fermentation batch test provided by the embodiment of the invention comprises the following steps:
s101, when fermentation is carried out, a sampling conduit is closed, methane is generated in a fermentation bottle, so that the internal air pressure of a system is increased, the gas enters a gas collecting and draining bottle along the conduit, and water in the gas collecting and draining bottle is drained into an open empty bottle;
s102, when liquid samples are taken, closing a first switch, blocking the communication of gas in a sampling catheter and an exhaust catheter, closing a second switch, blocking an air duct, opening the sampling catheter, and discharging the liquid in the system by extruding a balloon;
s103, as the sampling port guide pipe stretches into the liquid level, the liquid in the guide pipe plays a role of liquid seal, and external gas can be effectively prevented from entering the system;
s104, extruding the balloon to increase the air pressure of the fermentation bottle, and stably flowing out the liquid from the sampling catheter along the sampling catheter;
s105, when taking a gas sample, closing the sampling conduit, closing the second switch, opening the first switch to enable gas in the conduit to be communicated, at the moment, communicating the sampling conduit with the exhaust conduit, closing the second switch, releasing the balloon through continuous extrusion, enabling gas in the fermentation system to be fully and uniformly mixed, and taking the gas sample from the sampling conduit by using the sampling needle.
While the invention has been described with respect to what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but on the contrary, is intended to cover various modifications, equivalents, and alternatives falling within the spirit and scope of the invention.
Claims (7)
1. The experimental method for the gas-liquid co-sampling of the biogas fermentation batch test is characterized in that an experimental system for realizing the experimental method for the gas-liquid co-sampling of the biogas fermentation batch test is provided with:
a water collecting bottle;
the water collecting bottle is connected with the gas collecting and water discharging bottle through a first conduit, and a first rubber plug is plugged at the upper side of the gas collecting and water discharging bottle;
the gas-collecting drainage bottle is connected with the balloon through a second conduit, the balloon is connected with the fermentation bottle through an exhaust conduit, and the first switch is arranged at the joint of the second conduit and the balloon;
the upper side of the fermentation bottle is plugged with a second rubber plug, two through holes are formed in the middle of the second rubber plug, and a sampling conduit and an exhaust conduit are respectively arranged in the two through holes in a penetrating manner;
the fermentation bottle is filled with fermentation substrate and inoculum, the gas-collecting and water-draining bottle is filled with water, and the water-collecting bottle is an open empty bottle;
the sampling catheter is connected with the exhaust catheter through an air duct, and a second switch is arranged in the middle of the air duct;
the first rubber plug and the second rubber plug are fixed with a data line through the through hole in a sealing way;
the experimental method for the gas-liquid co-sampling in the biogas fermentation batch test comprises the following steps:
step one, when fermentation is carried out, a sampling conduit is closed, methane is generated in a fermentation bottle, so that the internal air pressure of a system is increased, the gas enters a gas collecting and draining bottle along the conduit, and water in the gas collecting and draining bottle is drained into an open empty bottle;
when liquid samples are taken, the first switch is closed, the communication of gas in the sampling catheter and the exhaust catheter is blocked, the second switch is closed, the gas guide tube is blocked, the sampling catheter is opened, and the liquid in the system is discharged by extruding the balloon;
step three, as the sampling port conduit extends below the liquid level, the liquid in the conduit plays a role of self-sealing and is used for preventing external gas from entering the system;
step four, extruding the balloon to increase the air pressure of the fermentation bottle, and stably flowing out the liquid along the sampling conduit from the sampling conduit;
and fifthly, when the gas sample is taken, closing the sampling conduit, closing the second switch, opening the first switch to enable the gas in the conduit to be communicated, at the moment, communicating the sampling conduit with the exhaust conduit, closing the second switch, releasing the balloon through continuous extrusion, enabling the gas in the fermentation system to be fully and uniformly mixed, and taking the gas sample from the sampling conduit by using the sampling needle.
2. The experimental method for co-sampling of gas and liquid in a biogas fermentation batch test according to claim 1, wherein the outer end of the sampling conduit is plugged with an ear-shaped silica gel plug, the outer part of the ear-shaped silica gel plug is provided with an overhanging annular ear, the middle of the ear-shaped silica gel plug is provided with two jacks for inserting glass tubes, and a table-shaped plug body is arranged below the ear-shaped silica gel plug.
3. The experimental method for gas-liquid co-sampling in a biogas fermentation batch test according to claim 1, wherein the lower end of the second rubber plug is fixedly provided with a gas pressure sensor.
4. The experimental method for co-sampling of biogas fermentation batch test gas and liquid according to claim 1, wherein a temperature sensor and a pH sensor are arranged inside the fermentation bottle, and the temperature sensor and the pH sensor are arranged outside the lower end of the sampling conduit.
5. The experimental method for gas-liquid co-sampling in a biogas fermentation batch test according to claim 1, wherein the experimental system for gas-liquid co-sampling in a biogas fermentation batch test further comprises:
the temperature detection module is connected with the central control module and detects the temperature inside the fermentation bottle through a temperature sensor;
the pH detection module is connected with the central control module and is used for detecting the pH value in the fermentation bottle through the pH sensor;
the air pressure detection module is connected with the central control module and detects the air pressure inside the fermentation bottle through the air pressure sensor;
the central control module is respectively connected with the temperature detection module, the gas detection module, the air pressure detection module, the data transmission module, the fermentation temperature control module, the data comprehensive analysis module, the alarm module and the display module, and coordinates the normal operation of each module.
6. The experimental method for gas-liquid co-sampling in a biogas fermentation batch test of claim 5, wherein the data transmission module is used for interactive transmission of data through a signal transmission device;
the cloud service module is connected with the data transmission module and processes the whole system data by utilizing a big data technology through a cloud server;
the fermentation temperature control module changes the temperature of the fermentation bottle through a temperature regulator; the data comprehensive analysis module processes the whole system data through a data processing program;
the alarm module is used for alarming through alarm equipment; the display module is used for displaying corresponding data through the display screen.
7. The experimental method for co-sampling gas and liquid in a biogas fermentation batch test according to claim 6, wherein the data comprehensive analysis module processes the whole system data through a data processing program, and the specific process is as follows:
establishing a data set by using data corresponding to a temperature detection module, a gas pressure detection module, a data transmission module, a fermentation temperature control module, a data comprehensive analysis module, an alarm module and a display module;
classifying the data in the data set, and extracting corresponding target features;
and obtaining the characteristic quantity of the fusion target through a fusion algorithm, and carrying out target classification and identification.
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