CN112794876B - Garlic polypeptide extraction system and process - Google Patents
Garlic polypeptide extraction system and process Download PDFInfo
- Publication number
- CN112794876B CN112794876B CN202110174065.8A CN202110174065A CN112794876B CN 112794876 B CN112794876 B CN 112794876B CN 202110174065 A CN202110174065 A CN 202110174065A CN 112794876 B CN112794876 B CN 112794876B
- Authority
- CN
- China
- Prior art keywords
- liquid nitrogen
- garlic
- ultra
- sample
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Abstract
The invention discloses an extraction system of garlic polypeptide, which relates to the technical field of extraction of garlic polypeptide and comprises a system bracket, wherein a liquid nitrogen freezing device is arranged on the system bracket, a powder making mechanism is arranged on one side of the liquid nitrogen freezing device, a PBS (phosphate buffer solution) device is arranged on one side of the powder making mechanism, an ultra-membrane filtering device is arranged on one side of the PBS device, a two-dimensional electrophoresis separating device is arranged on one side of the ultra-membrane filtering device, and a mass spectrometer is arranged on one side of the two-dimensional electrophoresis separating device; according to the invention, the ultra-membrane filter tube is connected to the guide tube of the injector in a screwing manner through the threads, so that a sample can be injected into the ultra-membrane filter tube, low-temperature cold air of liquid nitrogen in the liquid nitrogen tank is conveyed into the storage cabinet through the conveying pipe, and the low temperature in the storage cabinet meets the sample storage requirement, thereby being beneficial to improving the storage time of the sample.
Description
Technical Field
The invention belongs to the technical field of extraction of garlic polypeptide, and particularly relates to a system and a process for extracting garlic polypeptide.
Background
The whole plant of garlic has strong spicy garlic odor, the garlic bulb, garlic leaves and flower bolt can be eaten as vegetables, not only can be used as flavoring, but also can be used as medicine, and are famous plants for both food and medicine, the polypeptide in the garlic can be used for sterilization, and has the anti-tumor effect.
However, the adoption of the extraction method still has the following defects: 1. the installation of the ultrafiltration membrane tube is complex, a large amount of time is consumed for one-time filtration operation, and the efficiency is low; 2. after the sample is separated by the dielectrophoresis, the sample cannot be subjected to mass spectrum separation in time, so that the activity of the sample is reduced at normal temperature, the sample is deteriorated, and the extraction precision is reduced.
Disclosure of Invention
In view of this, the present invention aims to provide a system and a process for extracting garlic polypeptide, so as to effectively solve the problems that a lot of time is consumed for performing a filtering operation, and mass spectrometry cannot be performed on a sample in time, so that the activity of the sample is reduced at normal temperature, and the sample is deteriorated.
In order to achieve the purpose, the invention provides the following technical scheme: a garlic polypeptide extraction system comprises a system bracket, wherein a liquid nitrogen freezing device is mounted on the system bracket, a powder making mechanism is mounted on one side of the liquid nitrogen freezing device, a PBS (phosphate buffer solution) device is mounted on one side of the powder making mechanism, an ultra-membrane filtering device is mounted on one side of the PBS device, a two-dimensional electrophoresis separation device is mounted on one side of the ultra-membrane filtering device, and a mass spectrometer is mounted on one side of the two-dimensional electrophoresis separation device;
the ultra-membrane filtering device comprises a telescopic assembly, one end of the telescopic assembly penetrates through the system support, a piston is installed at the end of the telescopic assembly, a fixed plate is installed at the position, close to the bottom of the system support, below the telescopic assembly, an injector is installed at the bottom of the fixed plate, a guide pipe is installed at the end of the injector, and the end of the guide pipe is connected with an ultra-membrane filtering pipe.
Preferably, install cold storage plant between dielectrophoresis separator and the mass spectrograph, cold storage plant is including storing the cabinet, and stores the front surface mounting of cabinet and have the emergency exit, store one side of cabinet and install the liquid nitrogen container, and the top of liquid nitrogen container installs sealed lid, the conveyer pipe is installed to the tip of sealed lid.
Preferably, the heat insulation board is arranged inside the refrigerating device, and four metal boxes are arranged inside the refrigerating device.
Preferably, both ends of the metal box are rectangular openings, and the metal box is made of aluminum.
Preferably, liquid nitrogen is filled in the liquid nitrogen tank, the liquid nitrogen tank is made of stainless steel metal, and the liquid nitrogen tank is connected with the sealing cover in a screwing mode through threads.
Preferably, the internal thread pipe is installed on the top of ultra-membrane filter tube, the outer wall of pipe is provided with the external screw thread, the pipe closes with the internal thread pipe soon through the screw thread and is connected to realize being connected of ultra-membrane filter tube and syringe.
The extraction process of the garlic polypeptide based on the system comprises the following steps:
q1. taking out fresh Bulbus Allii from soil, peeling, and making into square block-shaped Bulbus Allii with square mold;
q2, putting the block garlic into a liquid nitrogen freezing device for freezing;
q3. taking out the frozen Bulbus Allii, placing the Bulbus Allii into powder making mechanism, and crushing Bulbus Allii into powder with the punch block of the powder making mechanism;
pouring garlic powder into a glass container, pouring pH. PBS solution in a PBS solution device into the glass container, and dissolving the garlic powder into the PBS solution;
q5. sucking with syringe; and filtering the garlic powder solvent by using an ultra-membrane filtering device to obtain a sample.
And Q6, performing two-dimensional electrophoresis separation on the sample by using a two-dimensional electrophoresis separation device, and after the two-dimensional electrophoresis separation of the sample, putting the sample into a refrigeration device for refrigeration and preparing the mass spectrometer.
And Q7, performing mass spectrum separation on the sample by adopting a mass spectrometer, and then extracting the polypeptide in the garlic.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the invention, the ultra-membrane filter tube is connected to the guide tube of the injector in a screwing manner through the threads, the piston is pushed by the telescopic assembly, and a sample in the injector is extruded into the ultra-membrane filter tube, so that the sample can be injected into the ultra-membrane filter tube, the ultra-membrane filter tube is convenient to disassemble and assemble, the sample filtering efficiency is ensured, and a worker can simply operate the filtering device.
(2) According to the invention, low-temperature cold air of liquid nitrogen in the liquid nitrogen tank is conveyed into the storage cabinet through the conveying pipe, the storage cabinet forms a refrigerated cabinet under the low-temperature effect, a sample is stored in the storage cabinet, the low temperature in the storage cabinet meets the sample storage requirement, the sample storage time is favorably prolonged, and a worker has sufficient time to prepare a mass spectrometer and then perform mass spectrum separation on the sample.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the refrigerator of the present invention;
FIG. 3 is a schematic structural view of an ultrafiltration membrane filtration device according to the present invention;
FIG. 4 is an enlarged view of portion A of FIG. 3;
FIG. 5 is an enlarged view of the portion B of FIG. 3;
in the figure: 1-a system support; 2-liquid nitrogen freezing device; 3-a powder making mechanism; 4-PBS solution device; 5-an ultra-membrane filtration device; 6-a dielectrophoresis separation device; 7-a refrigeration device; 71-a safety door; 72-a storage cabinet; 8-mass spectrometry; 9-liquid nitrogen tank; 10-sealing cover; 11-a delivery pipe; 12-a metal box; 13-heat insulation board; 14-an ultra-membrane filtration tube; 15-a telescoping assembly; 16-a syringe; 17-fixing the plate; 18-a piston; 19-an internally threaded tube; 20-conduit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides the following technical solutions: an extraction system of garlic polypeptide comprises a system bracket 1, wherein a liquid nitrogen freezing device 2 is installed on the system bracket 1, a powder making mechanism 3 is installed on one side of the liquid nitrogen freezing device 2, a PBS (phosphate buffer solution) device 4 is installed on one side of the powder making mechanism 3, an ultra-membrane filtering device 5 is installed on one side of the PBS device 4, a two-dimensional electrophoresis separating device 6 is installed on one side of the ultra-membrane filtering device 5, and a mass spectrometer 8 is installed on one side of the two-dimensional electrophoresis separating device 6;
the device comprises a liquid nitrogen freezing device 2, a powder making mechanism 3, a PBS solution device 4, an ultra-membrane filtering device 5, a two-dimensional electrophoresis separating device 6 and a mass spectrometer 8 which are all fixed on a system bracket 1, wherein the liquid nitrogen freezing device 2 is a liquid nitrogen containing cylinder, liquid nitrogen is filled in the liquid nitrogen containing cylinder, the top of the liquid nitrogen containing cylinder is sealed by a metal cover so as to keep the temperature of the liquid nitrogen, the powder making mechanism 3 drives an extrusion block through a cylinder, the extrusion block smashes the frozen garlic, and a neutral PBS solution is filled in the PBS solution device 4 and is used for dissolving garlic powder so as to obtain a garlic powder solvent;
the ultra-membrane filtering device 5 comprises a telescopic assembly 15, the telescopic assembly 15 can adopt an air cylinder or an electric telescopic rod, one end of the telescopic assembly 15 penetrates through the system support 1, a piston 18 is installed at the end part of the telescopic assembly 15, a fixing plate 17 is installed below the telescopic assembly 15 and close to the bottom of the system support 1, an injector 16 is installed at the bottom of the fixing plate 17, a guide pipe 20 is installed at the end part of the injector 16, the end part of the guide pipe 20 is connected with an ultra-membrane filtering pipe 14, a glass container is placed below the guide pipe 20 of the injector 16, the telescopic assembly 15 is used for driving the piston 18, garlic powder solvent in the glass container is sucked into the injector 16, then an internal threaded pipe 19 at the top end of the ultra-membrane filtering pipe 14 is connected onto the guide pipe 20 in a screwing manner through threads, the ultra-membrane filtering device 5 pushes the piston 18 through the telescopic assembly 15 so as to extrude garlic solvent in the injector 16, and presses the garlic solvent into the ultra-membrane filtering pipe 14 through pressure, the filtered garlic solvent flows out from the end of the ultra-membrane filter tube 14 to obtain a solvent, and a glass container is used for containing the sample solvent.
Preferably, a refrigerating device 7 is installed between the two-dimensional electrophoresis separation device 6 and the mass spectrometer 8, the refrigerating device 7 comprises a storage cabinet 72, a safety door 71 is installed on the front surface of the storage cabinet 72, a liquid nitrogen tank 9 is installed on one side of the storage cabinet 72, a sealing cover 10 is installed at the top of the liquid nitrogen tank 9, a conveying pipe 11 is installed at the end part of the sealing cover 10, and the two-dimensional electrophoresis separation device 6 separates proteins in a sample solvent, so that mass spectrometry separation is facilitated.
Preferably, refrigerating plant 7 internally mounted has heated board 13, and refrigerating plant 7's internally mounted has four metal box 12, and heated board 13 preserves the temperature of storing in the cabinet 72, guarantees that a stable temperature is used for cold-stored sample solution, and cold-stored sample in-process, the staff sets for the procedure on the mass spectrograph, then takes out cold-stored sample, and after ten minutes of stillness, carries out mass spectrum separation.
Preferably, the two ends of the metal box 12 are rectangular openings, and the metal box 12 is made of aluminum.
Preferably, liquid nitrogen is filled in the liquid nitrogen tank 9, the liquid nitrogen tank 9 is made of stainless steel metal, and the liquid nitrogen tank 9 is connected with the sealing cover 10 in a screwing mode through threads.
Preferably, the top end of the ultra-membrane filtration tube 14 is provided with an internal threaded tube 19, the outer wall of the conduit 20 is provided with external threads, and the conduit 20 is screwed with the internal threaded tube 19 to realize the connection between the ultra-membrane filtration tube 14 and the injector 16.
Aiming at the system provided by the invention, the invention also provides an extraction process of garlic polypeptide, which comprises the following steps:
q1. taking out fresh Bulbus Allii from soil, peeling, and making into square block-shaped Bulbus Allii with square mold;
q2, putting the block garlic into a liquid nitrogen refrigerating device 2 for freezing;
q3. taking out the frozen Bulbus Allii, placing the Bulbus Allii into the powder making mechanism 3, and crushing Bulbus Allii into powder with the punch block of the powder making mechanism 3;
pouring garlic powder into a glass container, pouring the PBS solution with the pH value of 7.0 in the PBS solution device 4 into the glass container, and dissolving the garlic powder into the PBS solution;
q5. aspirating with syringe 16; and filtering the garlic powder solvent by using an ultra-membrane filtering device 5 to obtain a sample.
And Q6, performing two-dimensional electrophoresis separation on the sample by using the two-dimensional electrophoresis separation device 6, and after the two-dimensional electrophoresis separation of the sample, putting the sample into a refrigerating device 7 for refrigeration and preparing a mass spectrometer 8.
And Q7, performing mass spectrum separation on the sample by adopting a mass spectrometer 8, and then extracting the polypeptide in the garlic.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. An extraction system of garlic polypeptide comprises a system support (1), wherein a liquid nitrogen freezing device (2) is installed on the system support (1), a powder making mechanism (3) is installed on one side of the liquid nitrogen freezing device (2), a PBS (phosphate buffer solution) device (4) is installed on one side of the powder making mechanism (3), an ultra-membrane filtering device (5) is installed on one side of the PBS solution device (4), a two-dimensional electrophoresis separating device (6) is installed on one side of the ultra-membrane filtering device (5), and a mass spectrometer (8) is installed on one side of the two-dimensional electrophoresis separating device (6);
the method is characterized in that: the ultrafiltration membrane filtering device (5) comprises a telescopic assembly (15), one end of the telescopic assembly (15) penetrates through the system support (1), a piston (18) is installed at the end part of the telescopic assembly (15), a fixing plate (17) is installed at the position, close to the bottom of the system support (1), below the telescopic assembly (15), an injector (16) is installed at the bottom of the fixing plate (17), a guide pipe (20) is installed at the end part of the injector (16), and the end part of the guide pipe (20) is connected with an ultrafiltration membrane filtering pipe (14);
internal thread pipe (19) are installed on the top of ultra-membrane filter tube (14), the outer wall of pipe (20) is provided with the external screw thread, pipe (20) close with internal thread pipe (19) soon through the screw thread and are connected to realize being connected of ultra-membrane filter tube (14) and syringe (16).
2. The extraction system of garlic polypeptide according to claim 1, wherein: install cold storage plant (7) between dielectrophoresis separator (6) and mass spectrometer (8), cold storage plant (7) are including storing cabinet (72), and the preceding surface mounting who stores cabinet (72) has emergency exit (71), liquid nitrogen container (9) are installed to one side of storing cabinet (72), and sealed lid (10) are installed at the top of liquid nitrogen container (9), conveyer pipe (11) are installed to the tip of sealed lid (10).
3. The extraction system of garlic polypeptide according to claim 2, wherein: the refrigerator is characterized in that the heat insulation plate (13) is arranged inside the refrigerator (7), and four metal boxes (12) are arranged inside the refrigerator (7).
4. The extraction system of garlic polypeptide according to claim 3, wherein: the two ends of the metal box (12) are rectangular openings, and the metal box (12) is made of aluminum.
5. The extraction system of garlic polypeptide according to claim 2, wherein: the liquid nitrogen tank (9) is internally provided with liquid nitrogen, the liquid nitrogen tank (9) is made of stainless steel metal, and the liquid nitrogen tank (9) is connected with the sealing cover (10) in a screwing mode through threads.
6. A garlic polypeptide extraction process, which is applicable to the garlic polypeptide extraction system of any one of claims 1-5, and comprises the following steps:
q1. taking out fresh Bulbus Allii from soil, peeling, and making into square block-shaped Bulbus Allii with square mold;
q2, putting the block garlic into a liquid nitrogen freezing device (2) for freezing;
q3. taking out the frozen Bulbus Allii, placing the Bulbus Allii into the powder making mechanism (3), and crushing Bulbus Allii into powder with the punch block of the powder making mechanism (3);
pouring garlic powder into a glass container, pouring the PBS solution with the pH value of 7.0 in the PBS solution device (4) into the glass container, and dissolving the garlic powder into the PBS solution;
q5. sucking garlic powder solvent with injector (16), and filtering with ultrafiltration membrane filter (5) to obtain sample;
and Q6, performing two-dimensional electrophoresis separation on the sample by using a two-dimensional electrophoresis separation device (6), and after the two-dimensional electrophoresis separation of the sample, putting the sample into a refrigeration device (7) for refrigeration and preparing a mass spectrometer (8);
and Q7, performing mass spectrum separation on the sample by adopting a mass spectrometer (8), and then extracting the polypeptide in the garlic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110174065.8A CN112794876B (en) | 2021-02-06 | 2021-02-06 | Garlic polypeptide extraction system and process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110174065.8A CN112794876B (en) | 2021-02-06 | 2021-02-06 | Garlic polypeptide extraction system and process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112794876A CN112794876A (en) | 2021-05-14 |
| CN112794876B true CN112794876B (en) | 2022-08-05 |
Family
ID=75814905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110174065.8A Active CN112794876B (en) | 2021-02-06 | 2021-02-06 | Garlic polypeptide extraction system and process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112794876B (en) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3290968B2 (en) * | 1998-04-30 | 2002-06-10 | フロンティア・ラボ株式会社 | Sample concentrator |
| CN101403561A (en) * | 2008-11-07 | 2009-04-08 | 中国林业科学研究院林产化学工业研究所 | Method and apparatus for atomizing, freezing and drying for powder production by using low-temperature low-dew point gas |
| CN107698664A (en) * | 2014-09-05 | 2018-02-16 | 马恒标 | The obtained polypeptide of antitumor activity is extracted in garlic |
| CN208791527U (en) * | 2018-06-19 | 2019-04-26 | 昆明医科大学 | Protein Extraction Filter column |
| US20200148996A1 (en) * | 2018-11-09 | 2020-05-14 | Covaris, Inc. | Cell lysis and biomolecule disassociation system and method for mass spectrometry or other analysis |
| CN109797184A (en) * | 2019-03-29 | 2019-05-24 | 集美大学 | A kind of crocodile peptide and its production method |
| CN111500438A (en) * | 2020-06-10 | 2020-08-07 | 扬州润明智能装备股份有限公司 | Vinasse polypeptide extraction equipment and extraction method |
-
2021
- 2021-02-06 CN CN202110174065.8A patent/CN112794876B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112794876A (en) | 2021-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10507407B2 (en) | Methods to reduce chlorophyll co-extraction through extraction of select moieties essential oils and aromatic isolates | |
| US20200080777A1 (en) | Herb essential oil processing equipment | |
| CN112794876B (en) | Garlic polypeptide extraction system and process | |
| CN202537964U (en) | Vacuum pumping refreshing feeding bottle | |
| CN103445137A (en) | Production technology of rose paste | |
| CN205358014U (en) | Portable fruit vegetables vacuum precooling machine | |
| CN203011058U (en) | Refrigerator with vacuum fresh-keeping device | |
| CN217755338U (en) | Conveying structure of food instant freezer | |
| CN102374724B (en) | Refrigerating plant with freeze-drying function | |
| CN203777388U (en) | Container for freezing and drying human platelet | |
| CN206496564U (en) | The fresh-keeping refrigerating plant of truffle | |
| CN211298339U (en) | Low-temperature dry distillation equipment for drying processing and collecting volatile liquid of fresh amomum tsao-ko | |
| CN109566713A (en) | A kind of the composite fresh-keeping device and composite fresh-keeping method of chilled chicken | |
| CN101718485B (en) | Method for drying or concentrating at near freezing temperature and device thereof | |
| CN105532641A (en) | Placenta preserving fluid and use method thereof | |
| CN201575661U (en) | Drying or concentrating device | |
| CN202361730U (en) | Refrigeration device having freeze-drying function | |
| CN209720472U (en) | A kind of potato storage energy-saving fresh-keeping storage | |
| CN208199156U (en) | Citrus vacuum freshness retaining transports combination box | |
| CN208620686U (en) | Quick cooler for the pre-cooling of fruits and vegetables prepared food | |
| CN216048725U (en) | Low temperature freeze-drying equipment for biological medicine experiments | |
| CN220032554U (en) | Big cherry commodity circulation preservation box | |
| CN220926697U (en) | Fish oil extraction element of fish offal | |
| CN206440069U (en) | A kind of food product refrigeration vacuum freezing equipment | |
| CN206056056U (en) | 40 DEG C of low-temp. refrigerators of single compression refrigeration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |