CN112779138A - Three-dimensional pipeline closed type edible microalgae culture device and use method thereof - Google Patents

Three-dimensional pipeline closed type edible microalgae culture device and use method thereof Download PDF

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Publication number
CN112779138A
CN112779138A CN202110268811.XA CN202110268811A CN112779138A CN 112779138 A CN112779138 A CN 112779138A CN 202110268811 A CN202110268811 A CN 202110268811A CN 112779138 A CN112779138 A CN 112779138A
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pipe
negative pressure
dimensional
communicated
spiral sealing
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罗光宏
陈天仁
周安善
刘海燕
杨生辉
王丽娟
王丹霞
崔岩
吴松宏
曹海
李海涛
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GANSU KAIYUAN BIOTECHNOLOGY DEVELOPMENT CENTER
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    • C12M41/26Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH

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Abstract

The invention discloses a three-dimensional pipeline closed type edible microalgae culture device and a use method thereof, wherein the three-dimensional pipeline closed type edible microalgae culture device comprises the following steps: a three-dimensional sealed culture device; the cooling spraying device is arranged above the three-dimensional sealed culture device; the vacuum positive and negative pressure matching unit is respectively communicated with the exhaust pipe and the three-dimensional sealed culture device through hoses; the intelligent numerical control instrument box is electrically connected with the three-dimensional sealed culture device; the spirulina harvester is communicated with the three-dimensional sealed culture device through a hose; the culture solution storage tank is communicated with the three-dimensional sealed culture device through a hose. The invention has the technical effects of small occupied area, no culture water pollution and quick and uniform water circulation in the device.

Description

Three-dimensional pipeline closed type edible microalgae culture device and use method thereof
Technical Field
The invention relates to the technical field of microalgae cultivation, in particular to a three-dimensional pipeline closed type edible microalgae cultivation device and a using method thereof.
Background
The algae plant is a kind of relatively primitive and old low-grade autotrophic organism, has simple structure, no root, stem and leaf differentiation, is mostly a single-cell, colony or multi-cell thallus, has large size difference, contains photosynthetic pigment and can perform photosynthesis. About 3 thousands of species of the plant are distributed in fresh water or seawater, and the phenomenon of red tide, water bloom and the like can be caused by excessive propagation of some species. At present, the existing spirulina cultivation mainly adopts greenhouse pool cultivation, the circulation of the spirulina liquid mainly adopts plate-type instantaneous stirring of the spirulina liquid for circulation, and then adopts an open spirulina cultivation mode in lakes and sea areas. The collection of spirulina is mainly carried out by manual washing, filtering, dripping and scraping, and the steps are complicated, time-consuming and labor-consuming. And the prior open type culture pond for the spirulina in the greenhouse has the technical problems of large occupied area, culture water pollution, slow and uneven water circulation in the device and difficult control of various indexes in the device.
Therefore, how to provide a three-dimensional pipeline closed type edible microalgae cultivation device with small floor area, no cultivation water pollution, fast and uniform water circulation inside the device and easily controlled indexes inside the device and a using method thereof is one of the technical problems to be solved in the field.
Disclosure of Invention
In view of the above, the invention provides a three-dimensional pipeline closed type edible microalgae cultivation device and a use method thereof.
In order to solve the technical problems, the invention adopts the following technical scheme:
a three-dimensional pipeline closed type edible microalgae cultivation device comprises:
the three-dimensional sealed culture device comprises a three-dimensional spiral sealed pipe, a negative pressure air suction pipe, an exhaust pipe, a communicating pipe and a negative pressure liquid return pipe; the three-dimensional spiral sealing pipe is of a frustum structure; the negative pressure air suction pipe is communicated with the upper end opening of the three-dimensional spiral sealing pipe; the exhaust pipe is vertically arranged on the inner side of the three-dimensional spiral sealing pipe, and one end of the exhaust pipe is communicated with the lower port of the three-dimensional spiral sealing pipe; the exhaust pipe is communicated with one end of the communicating pipe through a tee; the other end of the communicating pipe is communicated with the negative pressure air suction pipe; the negative pressure liquid return pipe is horizontally arranged above the communicating pipe and is communicated with the middle part of the communicating pipe;
the cooling spraying device is arranged above the three-dimensional spiral sealing pipe;
the vacuum positive and negative pressure matching unit is respectively communicated with the exhaust pipe and the three-dimensional spiral sealing pipe through hoses;
the intelligent numerical control instrument box is electrically connected with the three-dimensional spiral sealing pipe;
the spirulina harvester is communicated with the three-dimensional spiral sealing pipe through the hose;
and the culture solution storage tank is communicated with the three-dimensional spiral sealing pipe through the hose.
Preferably, the three-dimensional spiral sealing pipe is formed by sequentially communicating a plurality of transparent pipes, a plurality of connecting pipe beads and a plurality of bent pipes thereof.
The beneficial effect of this technical scheme is: the transparent tube can promote photosynthesis of cultured algae and can be connected with one or more transparent tubes to meet actual requirements.
Preferably, a negative pressure electromagnetic valve is arranged at the top end of the exhaust pipe; correspondingly, the vacuum positive and negative pressure matching unit is provided with an air suction port; the air suction port is communicated with the negative pressure electromagnetic valve through the hose.
Preferably, a positive pressure interface is arranged on one side of the lower end pipe of the three-dimensional spiral sealing pipe; correspondingly, an exhaust port is arranged on the vacuum positive and negative pressure matching unit; the exhaust port is communicated with the positive pressure interface through the hose.
The beneficial effect of this technical scheme is: the vacuum positive-negative pressure press-fit sleeve machine set pressurizes and fills gas into the positive pressure interface and sucks gas out of the negative pressure electromagnetic valve under negative pressure, so that gas flow in the three-dimensional spiral sealing pipe circulates from bottom to top.
Preferably, a liquid tank interface is arranged on the other side of the lower end pipe of the three-dimensional spiral sealing pipe and is communicated with the culture solution storage tank through the hose.
Preferably, a plurality of algae collecting ports are arranged at the bottom of the three-dimensional spiral sealing pipe, and each algae collecting port is communicated with the spiral algae collecting machine through the hose.
Preferably, the pipe wall outside of three-dimensional spiral sealed tube is provided with a plurality of apparatus probes, and is corresponding, be provided with a plurality of control display element on the intelligent numerical control instrument case, every apparatus probe one-to-one with every on the intelligent numerical control instrument case control display element electricity is connected.
Preferably, the plurality of determinator probes comprise chlorophyll fluorescence dynamics parameter determinator probe, pH value determinator probe, light intensity determinator probe, solution alkalinity determinator probe and temperature automatic controller probe; the control display units comprise a chlorophyll fluorescence kinetic parameter tester, a pH value tester, a light intensity tester, a pH value tester and a temperature automatic controller; the chlorophyll fluorescence kinetic parameter measuring instrument probe is electrically connected with the chlorophyll fluorescence kinetic parameter measuring instrument, the pH value measuring instrument probe is electrically connected with the pH value measuring instrument, the light intensity measuring instrument probe is electrically connected with the light intensity measuring instrument, the soluble alkalinity measuring instrument probe is electrically connected with the soluble alkalinity measuring instrument, and the temperature automatic controller probe is electrically connected with the temperature automatic controller.
Preferably, intelligence numerical control instrument box still is equipped with control the total electric switch of intelligence numerical control instrument box, monitoring voltmeter, the monitoring of intelligence numerical control instrument box voltage the ammeter, the control of intelligence numerical control instrument box electric current are a plurality of the electric control switch A, the control of apparatus probe are a plurality of control display element's electric control switch B.
The beneficial effect of this technical scheme is: the cultivation condition of algae in the device can be accurately monitored, so that scientific management and cultivation are facilitated.
Preferably, two sides of the connection position of the communicating pipe and the negative pressure liquid return pipe are respectively provided with a one-way valve.
The beneficial effect of this technical scheme is: the two one-way valves can be regulated and controlled according to actual conditions so as to control the flow direction of the airflow.
Preferably, the top end of the negative pressure air suction pipe is provided with a negative pressure air suction cylinder.
Preferably, one end of the negative pressure liquid return pipe is provided with an electromagnetic valve for discharging gas, and the other end of the negative pressure liquid return pipe is a sealing end.
The beneficial effect of this technical scheme is: the gas in the device can be exhausted through the negative pressure suction cylinder and the electromagnetic valve.
The invention also provides a use method of the three-dimensional pipeline closed type edible microalgae cultivation device, which comprises the following steps:
s1, firstly, inputting the culture solution in the culture solution storage tank into the three-dimensional spiral sealing pipe to provide nutrition for the growth of microalgae;
s2, pressurizing and filling gas into the positive pressure interface by using the vacuum positive-negative pressure press fit sleeve unit, and sucking gas out of the negative pressure solenoid valve under negative pressure, so that gas flow inside the three-dimensional spiral sealing pipe circulates from bottom to top;
s3, regulating and controlling the temperature of the three-dimensional sealed culture device through the cooling spray device;
s4, monitoring each index of the culture solution in the three-dimensional spiral sealing pipe by using the intelligent numerical control instrument box;
and S5, finally, connecting the concentrated algae liquid in the pipeline with a harvesting machine through a hose to automatically concentrate and clean the algae mud, so as to obtain a qualified fresh algae mud product.
Compared with the prior art, the invention has the following technical effects:
the three-dimensional spiral sealing pipe is of a frustum-shaped structure, so that the occupied area is effectively reduced; the device is integrally sealed, so that a suitable environment is effectively provided for the growth of algae; the culture device is communicated with the vacuum positive and negative pressure matching set unit, so that the water circulation in the culture device is faster and more uniform; and a plurality of measuring instrument probes are arranged on the culture device and are electrically connected with the intelligent numerical control instrument box, so that the culture condition of algae in the device can be accurately monitored, and scientific management and cultivation are facilitated.
Drawings
FIG. 1 is a schematic view of the overall structure of a three-dimensional pipeline closed type edible microalgae cultivation device according to the present invention;
FIG. 2 is a schematic structural view of a three-dimensional spiral sealing pipe of the three-dimensional pipeline closed type edible microalgae cultivation device of the present invention;
FIG. 3 is a schematic structural view of a vacuum positive-negative pressure matching unit of the three-dimensional pipeline closed type edible microalgae cultivation device of the present invention;
FIG. 4 is a schematic structural view of an intelligent numerical control instrument box of the three-dimensional pipeline closed type edible microalgae cultivation device.
In the figure: 1. a three-dimensional sealed culture device; 11. a three-dimensional spiral sealing tube; 111. a transparent tube; 112. connecting tube beads; 113. bending the pipe; 12. a negative pressure suction pipe; 13. an exhaust pipe; 14. a communicating pipe; 15. a negative pressure liquid return pipe; 16. a tee joint; 17. a negative pressure solenoid valve; 18. a positive pressure interface; 19. a liquid tank interface; 2. a cooling spray device; 21. a meter probe; 22. a hose; 3. a vacuum positive and negative pressure matching machine set; 31. an air suction port; 32. an exhaust port; 4. an intelligent numerical control instrument box; 5. a spirulina harvester; 6. and (5) a culture solution storage tank.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1-4, a three-dimensional pipeline closed type edible microalgae cultivation device comprises:
the three-dimensional sealed culture device 1 comprises a three-dimensional spiral sealed pipe 11, a negative pressure air suction pipe 12, an exhaust pipe 13, a communicating pipe 14 and a negative pressure liquid return pipe 15; the three-dimensional spiral sealing pipe 11 is a cone frustum structure and is a spiral body formed by sequentially communicating a plurality of transparent pipes, a plurality of connecting pipe beads and a plurality of bent pipes thereof; the outer side of the pipe wall of the three-dimensional spiral sealing pipe 11 is provided with a plurality of measuring instrument probes 21, and the plurality of measuring instrument probes 21 comprise chlorophyll fluorescence kinetic parameter measuring instrument probes, pH value measuring instrument probes, light intensity measuring instrument probes, solution alkalinity measuring instrument probes and temperature automatic control instrument probes; a positive pressure port 18 is arranged on one side of the lower end pipe of the three-dimensional spiral sealing pipe 11, and a liquid tank port 19 is arranged on the other side; the bottom of the three-dimensional spiral sealing pipe 11 is provided with a plurality of algae collecting openings 20; the negative pressure air suction pipe 12 is communicated with the upper end opening of the three-dimensional spiral sealing pipe 11; the exhaust pipe 13 is vertically arranged on the inner side of the three-dimensional spiral sealing pipe 11, and one end of the exhaust pipe 13 is communicated with the lower port of the three-dimensional spiral sealing pipe 11; the exhaust pipe 13 is communicated with one end of the communicating pipe 14 through a tee joint 16; the other end of the communicating pipe 14 is communicated with the negative pressure suction pipe 12; the negative pressure liquid return pipe 15 is horizontally arranged above the communicating pipe 14 and communicated with the middle part of the communicating pipe 14, and two sides of the joint of the communicating pipe 14 and the negative pressure liquid return pipe 15 are respectively provided with a one-way valve;
the cooling spray device 2 is arranged above the three-dimensional spiral sealing pipe 11, and the cooling spray device 2 is provided with a plurality of spray heads;
the vacuum positive and negative pressure matching machine set 3 is provided with an air suction port 31 and an air exhaust port 32, the air suction port 31 is communicated with the negative pressure electromagnetic valve 17 through a hose 22, and the air exhaust port 32 is communicated with the positive pressure interface 18 through the hose 22;
the intelligent numerical control instrument box 4 is provided with a plurality of control display units, probes of each tester are electrically connected with the control display units on the intelligent numerical control instrument box in a one-to-one correspondence mode, and the control display units comprise a chlorophyll fluorescence kinetic parameter tester, a pH value tester, a light intensity tester, a dissolved alkalinity tester and a temperature automatic controller; the probe of the chlorophyll fluorescence kinetic parameter measuring instrument is electrically connected with the chlorophyll fluorescence kinetic parameter measuring instrument, the probe of the pH value measuring instrument is electrically connected with the pH value measuring instrument, the probe of the light intensity measuring instrument is electrically connected with the light intensity measuring instrument, the probe of the soluble alkalinity measuring instrument is electrically connected with the soluble alkalinity measuring instrument, and the probe of the temperature automatic controller is electrically connected with the temperature automatic controller. The intelligent numerical control instrument box further comprises a total electric switch for controlling the intelligent numerical control instrument box, a voltmeter for monitoring the voltage of the intelligent numerical control instrument box, an ammeter for monitoring the current of the intelligent numerical control instrument box, an electric control switch A for controlling the plurality of measuring instrument probes, and an electric control switch B for controlling the plurality of control display units.
A spirulina harvesting machine 5, wherein the spirulina harvesting machine 5 is communicated with any spirulina harvesting port 20 through a hose 22;
the culture solution storage tank 6 is communicated with the liquid tank interface 19 through a hose 22;
in this embodiment, a negative pressure suction cylinder is provided at the top end of the negative pressure suction pipe 12.
In this embodiment, one end of the negative pressure liquid return pipe 15 is provided with an electromagnetic valve for exhausting gas, and the other end is a sealing end.
In this embodiment, a water pump is provided on the hose 22 connecting the culture solution tank 6 and the three-dimensional spiral sealing tube 11.
In some embodiments, there is also provided a use method of the stereoscopic pipeline closed type edible microalgae cultivation device, which comprises the following steps:
s1, firstly, the culture solution in the culture solution storage tank 6 is input into the three-dimensional spiral sealing pipe 11;
s2, pressurizing the positive pressure interface 18 by using the vacuum positive and negative pressure matching unit 3, filling gas, and sucking the gas out of the negative pressure solenoid valve 17 by negative pressure, so that the whole gas flow of the three-dimensional spiral sealing pipe 11 circulates from bottom to top;
s3, regulating and controlling the temperature of the three-dimensional sealed culture device 1 through the cooling and spraying device 2;
s4, monitoring various indexes of the culture solution in the three-dimensional spiral sealing pipe 11 by using the intelligent numerical control instrument box 4;
and S5, finally dredging the qualified product into the culture solution storage tank 6.
The working principle is as follows: the culture solution is input into the three-dimensional spiral sealing pipe, and the transparent pipe enables more light sources to enter the culture device to promote the growth of algae; then, the vacuum positive-negative pressure fit assembly is used for inflating the lower end of the three-dimensional spiral sealing pipe and sucking air from the upper end of the three-dimensional spiral sealing pipe, so that the whole airflow of the culture device is circulated from bottom to top, and the growth of algae is further promoted; then the temperature of the three-dimensional sealed culture device is regulated and controlled through the cooling and spraying device, and all indexes of culture solution in the three-dimensional spiral sealing pipe are monitored by matching with an intelligent numerical control instrument box, so that scientific and efficient culture is achieved.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the technical scope of the present invention, so that any minor modifications, equivalent changes and modifications made to the above embodiment according to the technical spirit of the present invention are within the technical scope of the present invention.

Claims (10)

1. A three-dimensional pipeline closed type edible microalgae culture device is characterized by comprising:
the three-dimensional sealed culture device (1) comprises a three-dimensional spiral sealed pipe (11), a negative pressure air suction pipe (12), an exhaust pipe (13), a communicating pipe (14) and a negative pressure liquid return pipe (15); the three-dimensional spiral sealing pipe (11) is of a frustum structure; the negative pressure air suction pipe (12) is communicated with the upper end opening of the three-dimensional spiral sealing pipe (11); the exhaust pipe (13) is vertically arranged on the inner side of the three-dimensional spiral sealing pipe (11), and one end of the exhaust pipe (13) is communicated with the lower port of the three-dimensional spiral sealing pipe (11); the exhaust pipe (13) is communicated with one end of the communicating pipe (14) through a tee joint (16); the other end of the communicating pipe (14) is communicated with the negative pressure air suction pipe (12); the negative pressure liquid return pipe (15) is horizontally arranged above the communicating pipe (14) and is communicated with the middle part of the communicating pipe (14);
the cooling spray device (2) is arranged above the three-dimensional spiral sealing pipe (11);
the vacuum positive and negative pressure matching unit (3), the vacuum positive and negative pressure matching unit (3) is respectively communicated with the exhaust pipe (13) and the three-dimensional spiral sealing pipe (11) through a hose (22);
the intelligent numerical control instrument box (4), the intelligent numerical control instrument box (4) is electrically connected with the three-dimensional spiral sealing pipe (11);
the spirulina harvester (5), the spirulina harvester (5) is communicated with the three-dimensional spiral sealing pipe (11) through the hose (22);
the culture solution storage tank (6) is communicated with the three-dimensional spiral sealing pipe (11) through the hose (22).
2. The device for cultivating edible microalgae in a three-dimensional pipeline closed type as claimed in claim 1, wherein the three-dimensional spiral sealing pipe (11) is formed by communicating a plurality of transparent pipes (111), a plurality of connecting pipe beads (112) and a plurality of bent pipes (113).
3. The stereoscopic pipeline closed type edible microalgae cultivation device as claimed in claim 1, wherein a negative pressure solenoid valve (17) is arranged at the top end of the exhaust pipe (13); correspondingly, an air suction port (31) is arranged on the vacuum positive and negative pressure matching unit (3); the suction port (31) is communicated with the negative pressure electromagnetic valve (17) through the hose (22).
4. The stereoscopic pipeline closed type edible microalgae cultivation device as claimed in claim 1, wherein one side of the lower end pipe of the stereoscopic spiral sealing pipe (11) is provided with a positive pressure interface (18); correspondingly, an exhaust port (32) is arranged on the vacuum positive and negative pressure matching unit (3); the exhaust port (32) is communicated with the positive pressure interface (18) through the hose (22).
5. The device for cultivating edible microalgae in a three-dimensional pipeline closed type as claimed in claim 1, wherein a liquid tank port (19) is disposed on the other side of the lower end pipe of the three-dimensional spiral sealing pipe (11), and the liquid tank port (19) is communicated with the culture solution storage tank (6) through a hose (22).
6. The stereoscopic pipeline closed type edible microalgae cultivation device as claimed in claim 1, wherein a plurality of algae collecting ports (20) are arranged at the bottom of the stereoscopic spiral sealing pipe (11), and each algae collecting port (20) is communicated with the spirulina collecting machine through the hose.
7. The stereoscopic pipeline closed type edible microalgae cultivation device as claimed in claim 1, wherein a plurality of determinator probes (21) are arranged on the outer side of the pipe wall of the stereoscopic spiral sealing pipe (11); correspondingly, a plurality of control display units are arranged on the intelligent numerical control instrument box (4); each determinator probe (21) is electrically connected with a corresponding control display unit on the intelligent numerical control instrument box (4).
8. The stereoscopic pipeline closed type edible microalgae cultivation device as claimed in claim 1, wherein two sides of the connection between the communicating pipe (14) and the negative pressure liquid return pipe (15) are respectively provided with a one-way valve.
9. The stereoscopic pipeline closed type edible microalgae cultivation device as claimed in claim 1, wherein one end of the negative pressure liquid return pipe (15) is provided with an electromagnetic valve for exhausting gas, and the other end is a sealed end.
10. A method for using the three-dimensional pipeline closed type edible microalgae cultivation device based on the claims 1-10, which is characterized by comprising the following steps:
s1, firstly, inputting the culture solution in the culture solution storage tank into the three-dimensional spiral sealing pipe to provide nutrition for the growth of microalgae;
s2, pressurizing and filling gas into the positive pressure interface by using the vacuum positive-negative pressure press fit sleeve unit, and sucking gas out of the negative pressure solenoid valve under negative pressure, so that gas flow inside the three-dimensional spiral sealing pipe circulates from bottom to top;
s3, regulating and controlling the temperature of the three-dimensional sealed culture device through the cooling spray device;
s4, monitoring each index of the culture solution in the three-dimensional spiral sealing pipe by using the intelligent numerical control instrument box;
and S5, finally, connecting the concentrated algae liquid in the pipeline with a harvesting machine through a hose to automatically concentrate and clean the algae mud, so as to obtain a qualified fresh algae mud product.
CN202110268811.XA 2021-03-12 2021-03-12 Three-dimensional pipeline closed type edible microalgae culture device and use method thereof Pending CN112779138A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110564586A (en) * 2019-09-18 2019-12-13 南昌绿锦茶疗食品有限公司 Pipeline negative pressure type photobioreactor
CN112358947A (en) * 2020-11-16 2021-02-12 河西学院 A pipelining device for producing eat little algae freshly
CN112391272A (en) * 2020-10-27 2021-02-23 北海市绿牌生物科技有限公司 Microalgae culture system and using method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110564586A (en) * 2019-09-18 2019-12-13 南昌绿锦茶疗食品有限公司 Pipeline negative pressure type photobioreactor
CN112391272A (en) * 2020-10-27 2021-02-23 北海市绿牌生物科技有限公司 Microalgae culture system and using method
CN112358947A (en) * 2020-11-16 2021-02-12 河西学院 A pipelining device for producing eat little algae freshly

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