CN112391272A - Microalgae culture system and using method - Google Patents
Microalgae culture system and using method Download PDFInfo
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- CN112391272A CN112391272A CN202011166742.3A CN202011166742A CN112391272A CN 112391272 A CN112391272 A CN 112391272A CN 202011166742 A CN202011166742 A CN 202011166742A CN 112391272 A CN112391272 A CN 112391272A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention discloses a microalgae culture system and a using method thereof, comprising the following steps: the hollow ring pipes of each layer are communicated through connecting pipes, and the control valve, the mixing pipe, the pressure release valve, the siphon pipe, the conduction valve, the drainage valve and the pipeline are all made of transparent materials such as transparent glass or transparent plastic; the control valve is opened, microalgae and nutrient solution are input into the hollow ring pipe on each layer, then the control valve is closed, the whole system is in a closed-loop system due to the fact that the hollow ring pipe is closed, even at high temperature, moisture in the hollow ring pipe obtains heat energy and does not cause evaporation of the moisture, the problem of moisture evaporation when the open equipment is used for cultivating the microalgae is solved, and the problem of loss of nutritional ingredients of the microalgae after high-temperature drying is further solved.
Description
Technical Field
The invention relates to a microalgae cultivation system and a using method thereof, belonging to the technical field of algae cultivation equipment.
Background
Microalgae is a rare algae organism with a 35 hundred million year life history and is a natural food; . The microalgae is the most abundant and comprehensive organism with the most abundant and comprehensive nutritional ingredients in the nature, is rich in high-quality protein, fatty acid of gamma-linolenic acid, carotenoid, vitamins and various trace elements such as iron, iodine, selenium, zinc and the like, has comprehensive and balanced nutrition and extremely high disease prevention and health care values, and is the most excellent food resource and future food for human in the future;
in the microalgae cultured by using open equipment, the moisture in the growing environment of the microalgae is difficult to control to evaporate in the open mode, and when water is deficient and dried at high temperature, the nutritional ingredients of the algae powder of the microalgae are greatly lost, such as the denaturation of protein, the oxidation of pigments, the nutrient loss caused by the Maillard reaction of saccharides and amines, the reduction of carotenoid and phycocyanin by more than twenty percent, and the almost complete loss of other heat-sensitive biological enzyme active substances.
Disclosure of Invention
In order to solve the technical problem, the invention provides a microalgae cultivation system.
The invention also provides a using method of the microalgae culture system.
The invention is realized by the following technical scheme.
The invention provides a microalgae cultivation system, which comprises: the device comprises a plurality of layers of hollow circular pipes which are not closed for microalgae growth, wherein the hollow circular pipes of each layer are communicated through a connecting pipe, a control valve is arranged on each layer of hollow circular pipe, and microalgae, nutrient solution, high-pressure gas and the like can be flushed into the hollow circular pipes of each layer through the control valves;
one end of the mixing pipe is communicated with the hollow ring pipe at the top layer, the other end of the mixing pipe is closed, and a pressure release valve is arranged on the mixing pipe and used for releasing oxygen pressure generated by the microalgae;
the siphon pipe is characterized by also comprising an siphon pipe, wherein one end of the siphon pipe is communicated with the mixing pipe, the other end of the siphon pipe is communicated with the hollow ring pipe of the bottom layer provided with the control valve, and the middle part of the siphon pipe is provided with a conduction valve;
the drainage valve is arranged on the hollow ring pipe at the bottom layer, and the pressure release valve and the drainage valve are all opened when the multi-layer hollow ring pipe is required to be cleaned; the pipelines are made of transparent materials such as transparent glass or transparent plastics.
Still include the support frame, the support frame comprises montant, horizontal pole, the vertical pole that is a plurality of, through vertical pole fixed connection between two montants are vertical, through horizontal pole fixed connection between two montants are horizontal, be fixed with the shelves pole to hollow ring pipe support on the montant.
The vertical rods are fixedly connected with the longitudinal rods, the vertical rods are fixedly connected with the transverse rods, and the vertical rods are fixedly connected with the stop rods in a welding mode, so that the connection firmness is improved.
The stop lever is vertically fixed on the vertical rod.
The two vertical rods are arranged in a relatively inclined mode, and the vertical rods are arranged in the ratio of the vertical projection length to the horizontal projection length: .
The use method of the microalgae cultivation system comprises the following steps:
opening a control valve, infusing microalgae and nutrient solution into each layer of hollow ring pipe, and then closing the control valve, wherein the hollow ring pipe is closed, so that the whole system is in a closed loop system;
opening a control valve, and flushing high-pressure carbon dioxide gas into the control valve to enable the algae liquid mixture to move from the hollow ring pipe at the bottom layer to the hollow ring pipe at the top layer upwards, and then enabling the microalgae attached to the inner wall of the hollow ring pipe to fall off when the gas is flushed in the mixing pipe;
and step three, opening the conduction valve instantly, enabling the algae liquid mixture to flow through the siphon pipe instantly from the mixing pipe by means of self gravity to reach the hollow ring pipe located at the accessory of the control valve, and enabling the microalgae to be fully mixed and contacted with the carbon dioxide gas in the nutrient solution during recycling due to the fact that the hollow ring pipe is provided with a gap by the high-pressure carbon dioxide gas flushed into the mixing pipe by the control valve.
The invention has the beneficial effects that: the control valve is opened, microalgae and nutrient solution are input into the hollow ring pipe on each layer, then the control valve is closed, the whole system is in a closed-loop system due to the fact that the hollow ring pipe is closed, even at high temperature, moisture in the hollow ring pipe obtains heat energy and does not cause evaporation of the moisture, the problem of moisture evaporation when the open equipment is used for cultivating the microalgae is solved, and the problem of loss of nutritional ingredients of the microalgae after high-temperature drying is further solved.
Drawings
FIG. 1 is a schematic left front view of the present invention;
FIG. 2 is a schematic view of the right rear perspective structure of the present invention;
in the figure: 1-hollow ring pipe; 2-connecting pipe; 3-a control valve; 4-a mixing tube; 5-a pressure relief valve; 6-an siphon tube; 7-a conduction valve; 8-a drain valve; 91-vertical rod; 92-a cross-bar; 93-a longitudinal bar; 94-gear lever. Detailed Description
The technical solution of the present invention is further described below, but the scope of the claimed invention is not limited to the described.
See fig. 1-2.
The invention relates to a microalgae cultivation system, which comprises: the device comprises a plurality of layers of hollow circular pipes 1 which are not closed for microalgae growth, wherein the hollow circular pipes 1 on each layer are communicated through connecting pipes 2, a control valve 3 is arranged on each layer of hollow circular pipe 1, and microalgae, nutrient solution, high-pressure gas and the like can be flushed into the hollow circular pipe 1 on each layer through the control valve 3;
the device also comprises a mixing pipe 4, one end of the mixing pipe 4 is communicated with the hollow ring pipe 1 at the top layer, the other end of the mixing pipe is closed, and a pressure release valve 5 is arranged on the mixing pipe 4 and used for releasing the oxygen pressure generated by the microalgae;
the device also comprises an siphon pipe 6, one end of the siphon pipe 6 is communicated with the mixing pipe 4, the other end of the siphon pipe is communicated with the hollow ring pipe 1 at the bottom layer of the installation control valve 3, and a conduction valve 7 is arranged in the middle of the siphon pipe 6;
the drainage valve 8 is arranged on the hollow ring pipe 1 at the bottom layer, and the pressure release valve 5 and the drainage valve 8 are all opened when the multi-layer hollow ring pipe 1 needs to be cleaned; the pipelines are made of transparent materials such as transparent glass or transparent plastics.
The control valve 3 is opened, microalgae and nutrient solution are input into the hollow circular pipe 1 of each layer, then the control valve 3 is closed, the whole system is in a closed loop system due to the closed hollow circular pipe 1, even at high temperature, moisture in the hollow circular pipe 1 obtains heat energy and does not evaporate, the problem of moisture evaporation when open equipment is used for cultivating microalgae is solved, and the problem of loss of nutritional ingredients of the microalgae after high-temperature drying is solved; opening the control valve 3, supplementing nutrient solution to the microalgae in the hollow ring pipe 1, simultaneously flushing high-pressure carbon dioxide gas into the control valve 3, making the algae-laden liquid mixture move from the hollow ring pipe 1 at the bottom layer to the hollow ring pipe 1 at the top layer, then at the mixing pipe 4, when the gas is flushed, the microalgae attached to the inner wall of the hollow ring pipe 1 can fall off, then opening the conduction valve 7 instantly, making the algae-laden liquid mixture flow through the siphon pipe 6 instantly from the mixing pipe 4 by means of self gravity to reach the hollow ring pipe 1 at the accessory of the control valve 3, because the hollow ring pipe 1 is generated with a gap by the high-pressure carbon dioxide gas flushed by the control valve 3, making the siphon pipe 4 generate siphon, and making the microalgae and the carbon dioxide gas in the nutrient solution fully mixed and contacted during recirculation.
The drainage valve 8 is an electric valve convenient to control or a manual valve with stable control reliability.
The pressure release valve 5 is an electric valve convenient to control or a manual valve with stable control reliability.
The control valve 3 is an electric valve convenient to control or a manual valve with stable control reliability.
The conduction valve is an electric valve convenient to control or a manual valve stable in control reliability.
Still include the support frame, the support frame comprises montant 91, horizontal pole 92, the vertical pole 93 that are a plurality of, and two montants 91 are vertical between through vertical pole 93 fixed connection, through horizontal pole 92 fixed connection between two montant 91 are horizontal, be fixed with the shelves pole 94 to hollow ring pipe 1 support on the montant 91.
The vertical rod 91 is fixedly connected with the longitudinal rod 93, the vertical rod 91 is fixedly connected with the transverse rod 92, and the vertical rod 91 is fixedly connected with the stop rod 94 in a welding mode, so that the connection firmness is improved.
The stop rod 94 is vertically fixed on the vertical rod 91.
Two montants 91 between the relative slope setting, montant 91 is 1000 at the ratio of vertical direction projection length and horizontal direction projection length: 3.
the use method of the microalgae cultivation system comprises the following steps:
opening a control valve 3, infusing microalgae and nutrient solution into a hollow ring pipe 1 of each layer, and then closing the control valve 3, wherein the hollow ring pipe 1 is closed, so that the whole system is in a closed loop system;
opening a control valve 3, and flushing high-pressure carbon dioxide gas into the control valve 3 to enable the algae liquid mixture to move from the hollow circular pipe 1 at the bottom layer to the hollow circular pipe 1 at the top layer upwards, and then enabling the microalgae attached to the inner wall of the hollow circular pipe 1 to fall off when the gas is flushed in a mixing pipe 4;
and step three, opening the conduction valve 7 instantly, enabling the algae liquid mixture to flow through the siphon pipe 6 instantly from the mixing pipe 4 by means of self gravity to reach the hollow ring pipe 1 located at the accessory of the control valve 3, and enabling the microalgae to be fully mixed and contacted with the carbon dioxide gas in the nutrient solution during recycling as the hollow ring pipe 1 generates gaps by the high-pressure carbon dioxide gas flushed into the mixing pipe by the control valve 3.
Claims (10)
1. A microalgae cultivation system, comprising: the device comprises a plurality of layers of hollow circular pipes (1) which are not closed and used for microalgae growth, wherein the hollow circular pipes (1) on each layer are communicated through connecting pipes (2), a control valve (3) is arranged on each layer of hollow circular pipe (1), and microalgae, nutrient solution, high-pressure gas and the like can be flushed into the hollow circular pipes (1) on each layer through the control valves (3);
the device is characterized by further comprising a mixing pipe (4), wherein one end of the mixing pipe (4) is communicated with the hollow ring pipe (1) at the top layer, the other end of the mixing pipe is closed, and a pressure release valve (5) is arranged on the mixing pipe (4) and used for releasing the oxygen pressure generated by the microalgae;
the device also comprises an siphon pipe (6), one end of the siphon pipe (6) is communicated with the mixing pipe (4), the other end of the siphon pipe is communicated with the hollow ring pipe (1) of the bottom layer of the control valve (3), and a conducting valve (7) is arranged in the middle of the siphon pipe (6);
the drainage valve (8) is arranged on the hollow ring pipe (1) at the bottom layer, and the pressure release valve (5) and the drainage valve (8) are all opened when the multi-layer hollow ring pipe (1) needs to be cleaned; the pipelines are all made of light-transmitting materials.
2. The microalgae cultivation system of claim 1, wherein: the drainage valve (8) is an electric valve or a manual valve.
3. The microalgae cultivation system of claim 1, wherein: the pressure release valve (5) is an electric valve or a manual valve.
4. The microalgae cultivation system of claim 1, wherein: the control valve (3) is an electric valve or a manual valve.
5. The microalgae cultivation system of claim 1, wherein: the conduction valve (7) is an electric valve or a manual valve.
6. The microalgae cultivation system of claim 1, wherein: still include the support frame, the support frame comprises montant (91), horizontal pole (92), vertical pole (93) that are a plurality of, through vertical pole (93) fixed connection between two montants (91) are vertical, through horizontal pole (92) fixed connection between two montants (91) are horizontal, be fixed with shelves pole (94) that support hollow ring pipe (1) on montant (91).
7. The microalgae cultivation system of claim 6, wherein: the vertical rod (91) is fixedly connected with the longitudinal rod (93), the vertical rod (91) is fixedly connected with the transverse rod (92), and the vertical rod (91) is fixedly connected with the stop rod (94).
8. The microalgae cultivation system of claim 6, wherein: the stop lever (94) is vertically fixed on the vertical rod (91).
9. The microalgae cultivation system of claim 6, wherein: the two vertical rods (91) are arranged in a relatively inclined mode, and the ratio of the vertical length to the horizontal length of the vertical rods (91) is 1000: 3.
10. a method of using the microalgae cultivation system of any of claims 1 to 6, comprising the steps of:
opening a control valve (3), infusing microalgae and nutrient solution into a hollow ring pipe (1) of each layer, and then closing the control valve (3), wherein the hollow ring pipe (1) is closed, so that the whole system is in a closed loop system;
opening the control valve (3), flushing high-pressure carbon dioxide gas into the control valve (3) to enable the algae liquid mixture to move from the hollow circular pipe (1) at the bottom layer to the hollow circular pipe (1) at the top layer upwards, and then enabling the microalgae attached to the inner wall of the hollow circular pipe (1) to fall off when the gas is flushed in the mixing pipe (4);
and step three, opening the conducting valve (7) instantly, enabling the algae liquid mixture to flow through the siphon pipe (6) from the mixing pipe (4) instantly by means of self gravity to reach the hollow ring pipe (1) located at the accessory of the control valve (3), and enabling the mixing pipe (4) to generate siphon due to the fact that the hollow ring pipe (1) is provided with a gap by high-pressure carbon dioxide gas flushed by the control valve (3), and enabling the microalgae to be fully mixed and contacted with the carbon dioxide gas in the nutrient solution during recycling.
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Application publication date: 20210223 |