CN112772567A - Amblyseius pinolensis and method for feeding rootlet robinella chinensis prey thereof - Google Patents
Amblyseius pinolensis and method for feeding rootlet robinella chinensis prey thereof Download PDFInfo
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- CN112772567A CN112772567A CN202110016848.3A CN202110016848A CN112772567A CN 112772567 A CN112772567 A CN 112772567A CN 202110016848 A CN202110016848 A CN 202110016848A CN 112772567 A CN112772567 A CN 112772567A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a method for feeding siphonosteix pinicola and a prey robine root mite thereof, in particular to a method for feeding siphonosteix pinicola and a prey robine root mite thereof, which comprises the following steps: the method comprises the following steps: selecting a proper and transparent plastic feeding box and a box cover, and poking an annular small hole on the plastic feeding box cover by using a needle to ensure that sufficient oxygen exists in the box. Compared with the existing predatory mite feeding method, the feeding method has simple operation steps, greatly simplifies the existing feeding method of predatory mites and prey mites thereof, has low requirement on equipment, has low cost required by the equipment, and is easy to obtain the used feed and low in cost; the neoseiulus pinosus bred by the method has high reproduction rate and wide application range, can be used for scientific research and can also be used for preventing and controlling the root mites in the field or the root mites in the stored food such as indoor potatoes and the like.
Description
Technical Field
The invention relates to the technical field of feeding methods, in particular to a method for feeding siphonosteix pinosus and a prey root mite thereof.
Background
Robin root mite, also known as Roche root mite, is a harmful root mite of Arthropoda, Arachnida, Euglena, Dermatophagidae, or Rhizopus. In recent years, pest mites mainly comprising root mites of Robin mites widely harm agricultural products such as onions, Chinese chives and potatoes in China, so that the yield of the agricultural products is reduced, and even the agricultural products cannot be planted. Meanwhile, due to the use of a large amount of chemical agents such as insecticides and acaricides, the Robingen mites are resistant to the chemical agents on one hand; on the other hand, the soil and the environment are polluted in a large amount, so that not only is the ecological balance destroyed, but also the human health is seriously threatened, and therefore, finding an environmentally-friendly acaricidal mode becomes an important agricultural problem.
Amblyseius siphonorum belonging to the family Amblyseidae, genus Amblyseius, and capable of predating harmful mites on plants, such as microapods of Robinia; the ecological civilization construction is promoted, the agricultural non-point source pollution prevention and control are enhanced, and the prevention and control of the root mites of the Robin by utilizing the Amblyseius siphonapterus Ching becomes a key biological prevention and control and green prevention and control technology; the technology is green and environment-friendly in the control of the Robin root mites, and does not cause chemical pollution to soil and environment.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a method for feeding neoseiulus pinorubidus and the prey root mite thereof, which has the advantages that compared with the previous feeding method for predatory mites, the method for feeding neoseiulus pinorubidus has simple operation steps, greatly simplifies the existing feeding method for predatory mites and the prey mites thereof, has low requirements on equipment, has low cost, and is easy to obtain feed and low in cost; the neoseiulus pinosus bred by the method has high reproduction rate and wide application range, can be used for scientific research and can also be used for preventing and controlling the root mites in the field or the root mites in the stored food such as indoor potatoes and the like.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for feeding Amblyseius pinolensis and its prey root mite comprises the following steps:
the method comprises the following steps: selecting a proper and transparent plastic feeding box and a box cover, and pricking an annular small hole on the plastic feeding box cover by using a needle to ensure that sufficient oxygen exists in the box;
step two: cleaning potatoes with clear water, peeling, cutting into rectangular blocks with proper size, and placing the rectangular blocks into the plastic feeding box in the step one;
step three: putting prey root mites into a feeding box containing potatoes as feed;
step four: when the prey root mites on the feed reach 80-100 heads/g, the predatory mite Phytoseilus persimilis is inoculated, and the density is 80-100 heads/kg;
step five: placing a feeding box filled with feed, predatory mite pyemoseiulus siphonostericus and prey root mite into a phytotron for feeding, wherein the feeding conditions are as follows: the temperature is 20-25 ℃, the relative humidity is 70-80%, and the light-dark ratio is 12: 12 h;
step six: when the density of the predatory mite, the siphonosmus persicus, on each gram of feed reaches 120 heads/g, the predatory mite, the siphonosmus persicus can be used for laboratory internal scientific research, field control or low-temperature refrigeration for standby.
Preferably, the plastic rearing boxes used in the first step have a bottom diameter ranging from 30 to 70mm, a top diameter ranging from 30 to 70mm and a height ranging from 20 to 45mm, wherein the bottom diameter is 57mm, the top diameter is 70mm and the height is 45 mm.
Preferably, the potatoes used in the second step are used as feed for the prey root mites, and do not need to be replaced or added within a certain period of time.
Preferably, the root mites used in the third step are from leek, garlic root pseudostem and bulb parts collected in Pudingcounty, Anshun, Guizhou province.
Preferably, the amblyseius siphonapterus connected in the fourth step is from leek, garlic root pseudostem and bulb parts collected in Pudingcounty of Anshu city, Guizhou province.
Preferably, the predatory mite Physeiilus Diabrosus bred in the sixth step can be stored in a refrigerated container at a low temperature of-10 ℃ for standby.
Preferably, the predatory mite Physeiilus Diabrosus bred in the sixth step can be applied to scientific research in laboratories.
Preferably, the amblyseius pinosensis predatory mite bred in the sixth step can be applied to control of the root mites in the crop fields such as Chinese chives, scallion, garlic and the like.
Preferably, the amblyseius siphonaptonius predatory mite bred in the sixth step can be used for preventing and controlling the root mites of robins in stored grains such as potatoes, sweet potatoes and the like.
The invention has the beneficial effects that:
(1) the feeding device used in the method only comprises a plastic feeding box with the bottom diameter of 57mm, the top diameter of 70mm and the height of 45mm, compared with the existing predatory mite feeding method, the method has the advantages of low requirement on equipment, easiness in obtaining of the equipment and low price.
(2) The feed used by the method is the potato which is easy to obtain and has low cost.
(3) The method greatly improves the reproduction rate of the Phytoseilus nelumbinis.
(4) The amblyseius pinicola cultured by the method can be used for scientific research in laboratories, and can also be applied to the control of the root mites of the crops such as leeks, shallots, garlic and the like in the field or the control of the root mites of the crops such as potatoes and the like in storage.
In conclusion, compared with the existing predatory mite feeding method, the feeding method has simple operation steps, greatly simplifies the existing feeding method for the predatory mites and the prey mites thereof, has low requirement on equipment, has low cost required by the equipment, and has easily obtained and low cost of the used feed; the neoseiulus pinosus bred by the method has high reproduction rate and wide application range, can be used for scientific research and can also be used for preventing and controlling the root mites in the field or the root mites in the stored food such as indoor potatoes and the like.
Drawings
FIG. 1 is a perspective view of a plastic rearing box of Amblyseius pindus and its prey Rhizopus robinidae;
FIG. 2 is a perspective view of the plastic rearing box and the box cover in the rearing method of Amblyseius pindus pinicola and its prey, root mites.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1
The embodiment provides a method for feeding Phytoseilus nelumbinis and a prey Rhizopus robinii thereof, which comprises the following steps:
the method comprises the following steps: selecting a proper and transparent plastic feeding box and a box cover, and pricking an annular small hole on the plastic feeding box cover by using a needle to ensure that sufficient oxygen exists in the box;
step two: cleaning potatoes with clear water, peeling, cutting into rectangular blocks with proper size, and placing the rectangular blocks into the plastic feeding box in the step one;
step three: putting prey root mites into a feeding box containing potatoes as feed;
step four: when the prey root mites on the feed reach 80-100 heads/g, the predatory mite Phytoseilus persimilis is inoculated, and the density is 80-100 heads/kg;
step five: placing a feeding box filled with feed, predatory mite pyemoseiulus siphonostericus and prey root mite into a phytotron for feeding, wherein the feeding conditions are as follows: the temperature is 20-25 ℃, the relative humidity is 70-80%, and the light-dark ratio is 12: 12 h;
step six: when the density of the predatory mite, the siphonosmus persicus, on each gram of feed reaches 120 heads/g, the predatory mite, the siphonosmus persicus can be used for laboratory internal scientific research, field control or low-temperature refrigeration for standby.
In the invention, the plastic breeding box used in the first step has the diameter range of 30-70mm at the bottom, the diameter range of 30-70mm at the top and the height range of 20-45mm, wherein the diameter of the bottom is 57mm, the diameter of the top is 70mm and the height is 45 mm.
In the invention, the potatoes used in the step two are used as the feed of the prey root mites, and do not need to be replaced or added within a certain period of time.
In the invention, the root mites of the robine used in the third step are from the parts of leeks, garlic roots, pseudostems and bulbs collected from Pudingcounty of Anshun city, Guizhou province.
In the invention, the root mites of the robine used in the third step are from the parts of leeks, garlic roots, pseudostems and bulbs collected from Pudingcounty of Anshun city, Guizhou province.
In the implementation, the predatory mite Physeiilus Diabrosus bred in the sixth step can be stored in a refrigerated container at a low temperature of-10 ℃ for standby.
Example 2
The embodiment provides a method for feeding Phytoseilus nelumbinis and a prey Rhizopus robinii thereof, which comprises the following steps:
the method comprises the following steps: selecting a proper and transparent plastic feeding box and a box cover, and pricking an annular small hole on the plastic feeding box cover by using a needle to ensure that sufficient oxygen exists in the box;
step two: cleaning potatoes with clear water, peeling, cutting into rectangular blocks with proper size, and placing the rectangular blocks into the plastic feeding box in the step one;
step three: putting prey root mites into a feeding box containing potatoes as feed;
step four: when the prey root mites on the feed reach 80-100 heads/g, the predatory mite Phytoseilus persimilis is inoculated, and the density is 80-100 heads/kg;
step five: placing a feeding box filled with feed, predatory mite pyemoseiulus siphonostericus and prey root mite into a phytotron for feeding, wherein the feeding conditions are as follows: the temperature is 20-25 ℃, the relative humidity is 70-80%, and the light-dark ratio is 12: 12 h;
step six: when the density of the predatory mite, the siphonosmus persicus, on each gram of feed reaches 120 heads/g, the predatory mite, the siphonosmus persicus can be used for laboratory internal scientific research, field control or low-temperature refrigeration for standby.
In the invention, the plastic breeding box used in the first step has the diameter range of 30-70mm at the bottom, the diameter range of 30-70mm at the top and the height range of 20-45mm, wherein the diameter of the bottom is 57mm, the diameter of the top is 70mm and the height is 45 mm.
In the invention, the potatoes used in the step two are used as the feed of the prey root mites, and do not need to be replaced or added within a certain period of time.
In the invention, the root mites of the robine used in the third step are from the parts of leeks, garlic roots, pseudostems and bulbs collected from Pudingcounty of Anshun city, Guizhou province.
In the invention, the root mites of the robine used in the third step are from the parts of leeks, garlic roots, pseudostems and bulbs collected from Pudingcounty of Anshun city, Guizhou province.
In this embodiment, the predatory mite amblyseius pinoselium ensii raised in the sixth step can be applied to scientific research in laboratories.
Example 3
The embodiment provides a method for feeding Phytoseilus nelumbinis and a prey Rhizopus robinii thereof, which comprises the following steps:
the method comprises the following steps: selecting a proper and transparent plastic feeding box and a box cover, and pricking an annular small hole on the plastic feeding box cover by using a needle to ensure that sufficient oxygen exists in the box;
step two: cleaning potatoes with clear water, peeling, cutting into rectangular blocks with proper size, and placing the rectangular blocks into the plastic feeding box in the step one;
step three: putting prey root mites into a feeding box containing potatoes as feed;
step four: when the prey root mites on the feed reach 80-100 heads/g, the predatory mite Phytoseilus persimilis is inoculated, and the density is 80-100 heads/kg;
step five: placing a feeding box filled with feed, predatory mite pyemoseiulus siphonostericus and prey root mite into a phytotron for feeding, wherein the feeding conditions are as follows: the temperature is 20-25 ℃, the relative humidity is 70-80%, and the light-dark ratio is 12: 12 h;
step six: when the density of the predatory mite, the siphonosmus persicus, on each gram of feed reaches 120 heads/g, the predatory mite, the siphonosmus persicus can be used for laboratory internal scientific research, field control or low-temperature refrigeration for standby.
In the invention, the plastic breeding box used in the first step has the diameter range of 30-70mm at the bottom, the diameter range of 30-70mm at the top and the height range of 20-45mm, wherein the diameter of the bottom is 57mm, the diameter of the top is 70mm and the height is 45 mm.
In the invention, the potatoes used in the step two are used as the feed of the prey root mites, and do not need to be replaced or added within a certain period of time.
In the invention, the root mites of the robine used in the third step are from the parts of leeks, garlic roots, pseudostems and bulbs collected from Pudingcounty of Anshun city, Guizhou province.
In this embodiment, the amblyseius pinoselium persicaria predatory mite bred in the sixth step can be applied to the control of the root mites of the crops such as Chinese chives, shallots and garlic in the field.
Example 4
The embodiment provides a method for feeding Phytoseilus nelumbinis and a prey Rhizopus robinii thereof, which comprises the following steps:
the method comprises the following steps: selecting a proper and transparent plastic feeding box and a box cover, and pricking an annular small hole on the plastic feeding box cover by using a needle to ensure that sufficient oxygen exists in the box;
step two: cleaning potatoes with clear water, peeling, cutting into rectangular blocks with proper size, and placing the rectangular blocks into the plastic feeding box in the step one;
step three: putting prey root mites into a feeding box containing potatoes as feed;
step four: when the prey root mites on the feed reach 80-100 heads/g, the predatory mite Phytoseilus persimilis is inoculated, and the density is 80-100 heads/kg;
step five: placing a feeding box filled with feed, predatory mite pyemoseiulus siphonostericus and prey root mite into a phytotron for feeding, wherein the feeding conditions are as follows: the temperature is 20-25 ℃, the relative humidity is 70-80%, and the light-dark ratio is 12: 12 h;
step six: when the density of the predatory mite, the siphonosmus persicus, on each gram of feed reaches 120 heads/g, the predatory mite, the siphonosmus persicus can be used for laboratory internal scientific research, field control or low-temperature refrigeration for standby.
In the invention, the plastic breeding box used in the first step has the diameter range of 30-70mm at the bottom, the diameter range of 30-70mm at the top and the height range of 20-45mm, wherein the diameter of the bottom is 57mm, the diameter of the top is 70mm and the height is 45 mm.
In the invention, the potatoes used in the step two are used as the feed of the prey root mites, and do not need to be replaced or added within a certain period of time.
In the invention, the root mites of the robine used in the third step are from the parts of leeks, garlic roots, pseudostems and bulbs collected from Pudingcounty of Anshun city, Guizhou province.
In the invention, the root mites of the robine used in the third step are from the parts of leeks, garlic roots, pseudostems and bulbs collected from Pudingcounty of Anshun city, Guizhou province.
In this embodiment, the amblyseius pinkeyi predatory mite raised in the sixth step can be used for preventing and controlling robinid mites in stored grains such as potatoes.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent substitutions or changes according to the technical solution and the inventive concept of the present invention should be covered by the scope of the present invention.
Claims (9)
1. A method for feeding Amblyseius pinolensis and a prey root mite thereof is characterized by comprising the following steps:
the method comprises the following steps: selecting a proper and transparent plastic feeding box and a box cover, and poking an annular small hole on the plastic feeding box cover by using a needle to ensure that sufficient oxygen exists in the box;
step two: cleaning potatoes with clear water, peeling, cutting into rectangular blocks with proper size, and placing the rectangular blocks into the plastic feeding box in the step one;
step three: putting prey root mites into a feeding box containing potatoes as feed;
step four: when the prey root mites on the feed reach 80-100 heads/g, the predatory mite Phytoseilus persimilis is inoculated, and the density is 80-100 heads/kg;
step five: placing a feeding box filled with feed, predatory mite pyemoseiulus siphonostertanus and prey root mite into a phytotron for feeding, wherein the feeding conditions are as follows: the temperature is 20-25 ℃, the relative humidity is 70-80%, and the light-dark ratio is 12: 12 h;
step six: when the density of the predatory mite, namely the amblyseius pinosensis per gram of feed reaches 120 heads/g of 100-.
2. The method for rearing Amblyseius pinicola and the rhynchophorus sibiricus as claimed in claim 1, wherein the plastic rearing box used in the first step has a bottom diameter of 30-70mm, a top diameter of 30-70mm, a height of 20-45mm, wherein the bottom diameter is 57mm, the top diameter is 70mm, and the height is 45 mm.
3. The method for rearing Amblyseius siphonospius and its prey Rhizopus chinensis as claimed in claim 1, wherein the potato used in step two is used as feed for the prey Rhizopus chinensis without replacement or addition for a certain period of time.
4. The method for rearing Amblyseius siphonaptera and the prey root mite thereof as claimed in claim 1, wherein the root mite for Siblyseius siphonaptera used in the third step is selected from leek, garlic root pseudostem and bulb part collected in Pudingcounty, Anshun, Guizhou.
5. The method for rearing Amblyseius pindus dianus and the root mite of Robingen thereof as claimed in claim 1, wherein the Amblyseius pinkisii Linnaeus inserted in step four is selected from Allium tuberosum, Allium sativum root pseudostem, and bulb part collected in Pudingcounty of Anshu city, Guizhou.
6. The method for rearing Amblyseius pindus and its prey Rhizopus robinidae as claimed in claim 1, wherein the Amblyseius pindus predatory mite can be kept in a refrigerated container at a low temperature of-10 deg.C for use.
7. The method for rearing Amblyseius pindus and its prey Rhizopus robinidae as claimed in claim 1, wherein the Amblyseius pindus predatory mite bred in the sixth step can be applied to scientific research in laboratories.
8. The method for rearing Amblyseius pindus pinicola and the prey Robingen mite of the Amblyseius pinicola according to claim 1, wherein the Amblyseius pinicola reared in the sixth step can be applied to the control of the Robingen mites in the fields of crops such as Chinese chives, shallots, garlic and the like.
9. The method for rearing Amblyseius pindus pinicola and the prey Rhizopus rotundus thereof as claimed in claim 1, wherein the Amblyseius pinuscolor predatory mite reared in the sixth step can be used for controlling the Rhizopus pinbergii in stored food such as potato.
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