CN112760254A - Method for preventing and treating tomato bacterial wilt - Google Patents
Method for preventing and treating tomato bacterial wilt Download PDFInfo
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- CN112760254A CN112760254A CN202110081582.0A CN202110081582A CN112760254A CN 112760254 A CN112760254 A CN 112760254A CN 202110081582 A CN202110081582 A CN 202110081582A CN 112760254 A CN112760254 A CN 112760254A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/25—Paenibacillus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention relates to the field of agricultural microorganisms, and discloses a method for preventing and treating tomato bacterial wilt, which comprises the steps of performing root irrigation treatment on tomatoes by using a microbial preparation; the microbial preparation comprises an adsorbent and Paenibacillus jimila (B.jimmatis) (R)Paenibacillus jamilae) W51, wherein the total viable bacteria concentration of Paenibacillus jimmalaensis W51 is 1 x 108~1×109CFU/mL. Has better effect on preventing and controlling the tomato bacterial wilt, and the prevention effect reaches 79.35 percent.
Description
Description of the cases
The invention is a divisional application with application date of 2019, 9 and 29, application number of 2019109339644 and the name of Paenibacillus jimmara, a microbial preparation thereof, a preparation method and application thereof.
Technical Field
The invention relates to the field of agricultural microorganisms, in particular to a method for preventing and treating tomato bacterial wilt.
Background
The tomato is one of the most important vegetable planting crops in the production and planting system of China, the production scale of the tomato is continuously enlarged in recent years, the first disease causing the yield reduction of the tomato is tomato bacterial wilt in the cultivation and planting process, and the pathogenic bacteria is solanaceous Lawsonia (L) in the solanaceaeRalstonia solanacearum). The bacterial wilt can invade and hide from the skin holes or wounds of the roots and stem bases of the tomatoes by means of rainwater, irrigation water, underground insect pests or operating tools, and the like, quickly propagate in the vascular bundles when the conditions are proper, and expand upwards along the conduit to invade adjacent parenchyma cell tissues, so that the conduit is blocked, and the plants cannot obtain the corresponding water and nutrition supply and wither and die. Because the disease is extremely high in infectivity, pathogenic bacteria mainly complete overwintering and infection in soil, and the pathogenic mode of the pathogenic bacteria in a plant vascular bundle makes the traditional prevention and control means untreatable, so that the disease is called as 'plant cancer'. The area of south of the Yangtze river, China, particularly south China, is a serious disaster area of the diseaseOnce the disease is difficult to control, the tomato plants are often withered and die in a large area, the yield of the heavily diseased field is seriously reduced, even the tomato plants are completely harvested, and the disease seriously restricts the development of the tomato industry and the improvement of the economic benefit.
At present, a plurality of reports on the control of tomato bacterial wilt, such as pesticide irrigation, crop rotation, resistance breeding and the like, are provided, but stable and effective control measures are not provided. In recent years, more and more researchers begin to search for breakthrough in biological control, such as pseudomonas, bacillus, fungi, phage and the like, and since bacillus is an antagonistic bacterium of various pathogenic bacteria, the bacillus can prevent and control crop diseases and is generally considered to be an environment-friendly, economic and effective disease control way. However, although the current biological control preparations used at home and abroad have made a certain research progress on the control of tomato bacterial wilt, most biological control preparations are still in the experimental stage, and have many problems in practical application, such as unsatisfactory field control effect, short control time, low colonization ability of biological control bacteria, large environmental influence factors and the like.
Thus, suitable carrier materials are needed to enhance their adaptability and effectiveness. Clay minerals such as illite and the like are the main minerals constituting claystone and soil, and the large specific surface area, porous structure and strong adsorption property of clay particles have attracted increasing interest to researchers and researchers. Illite is a common clay mineral, and mainly comprises illite, and also contains a small amount of kaolinite, montmorillonite, chlorite, pyrophyllite and the like. Illite crystal structure belongs to 2: dioctahedral type with 1-type unit layers containing a large amount of metal cations such as Fe2+、Mg2+、Si2+Etc. can be combined with peptidoglycan on the surface of bacterial cell walls. In addition, the characteristics of large specific surface area of the illite crystal also enable the illite crystal to have good electrostatic adsorption performance, the characteristics enable the illite to gather bacterial thalli in water to form granular substances rich in the thalli, and most bacilli can form a bacterial membrane when being enriched, so that the thalli have higher survival rate in a poor environment than single thalli. In production application, the survival rate of the biocontrol bacteria around the plant root system can be directlyThe biocontrol effect is influenced, so that the thallus adsorbed by the illite has higher survival rate compared with single thallus, thereby having higher biocontrol effect.
The method has the advantages that the illite is utilized to adsorb the biocontrol bacteria to prepare the microbial preparation, and the microbial preparation is used for preventing and treating the tomato bacterial wilt, so that the survival rate of the biocontrol bacteria in the natural environment is expected to be improved, the colonization capability of the biocontrol bacteria around the tomato root system is improved, and the tomato disease influence caused by the bacterial wilt infection is prevented or alleviated. Therefore, the microbial preparation has considerable production and application prospects.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the problems in the prior art, the invention provides a method for preventing and treating tomato bacterial wilt, which has a higher prevention function on the tomato bacterial wilt.
The technical scheme is as follows: the invention provides Paenibacillus jimila, which is classified and namedPaenibacillus jamilaeW51, deposited in China general microbiological culture Collection center in 26 months and 10 months in 2018, wherein the deposit is No. 3 of Xilu-Beichen province in the rising area of Beijing, and the collection number of the strain is CGMCC No.16639 at the institute of microbiology of China academy of sciences.
The invention also provides application of Paenibacillus jimila in preventing and treating tomato bacterial wilt.
The present invention also provides a microbial preparation comprising an adsorbent and Paenibacillus jimiraensis W51 as set forth in claim 1; wherein the total viable bacteria concentration of Paenibacillus jimmara W51 is 1 x 108 ~1×109 CFU/mL。
Preferably, the adsorbent is illite powder.
Preferably, the concentration of the illite powder in the microbial preparation is 4%.
Preferably, the particle size of the illite powder is less than 2 μm, and the natural whiteness is greater than 85%.
The invention also provides a preparation method of the microbial preparation, which comprises the following steps: s1: inoculating seed bacterial liquid of Paenibacillus jimila W51 into PDB fermentation culture medium in a volume ratio of 1:100 for fermentation culture; the conditions of fermentation culture are as follows: carrying out shaking culture at the temperature of 32 ℃ and the rpm of 200-220 for 18-32 h; s2: centrifuging at 4000-6000 rpm for 25-30 min, and taking the precipitate and re-suspending with sterilized water to make the OD value range of the prepared bacterial suspension at 600 nm be 0.8-1.0; s3: and adding 4% of sterilized illite powder into the bacterial suspension, and carrying out oscillatory adsorption at 32 ℃ and 200-220 rpm for 0.5-1.0 h to obtain the microbial preparation.
Further, in S1, a seed bacterial liquid of paenibacillus jimmalaensis W51 was prepared as follows: inoculating Paenibacillus jimila W51 strain into YPGB culture solution at 32 deg.C, shaking at 200 rpm for 12 h, sampling in a clean bench every 2 h, measuring its OD value at 600 nm, and culturing when OD value is 0.8 to obtain seed bacterial liquid.
The invention also provides application of the microbial preparation in preventing and treating tomato bacterial wilt.
The invention also provides a method for preventing and treating tomato bacterial wilt, which uses a microbial preparation to perform root irrigation treatment on tomatoes.
Has the advantages that: the invention is a microbial preparation specially developed for tomato bacterial wilt, and because the microbial preparation is a biological preparation, a series of problems caused by the use of chemical pesticides are completely avoided, and the microbial preparation is not easy to generate resistance; the method is safe to the environment, pollution-free and residue-free; strong specificity, high activity, low production cost and simple preparation process, thereby being beneficial to the pollution-free production of tomatoes; the illite powder is a cheap natural clay mineral resource, so that farmers can not use or reduce the dosage of other chemical pesticides, thereby not only saving the expenditure of the farmers, but also reducing the residue of chemical agents.
The greenhouse experiment structure shows that: the Paenibacillus jimila W51 (CGMCC No. 16639) microbial preparation can obviously reduce the occurrence of tomato bacterial wilt. The root irrigation treatment is carried out on the microbial preparation prepared by the Paenibacillus jimela W51 and the illite powder, and the control effect on the tomato bacterial wilt can reach 79.35 percent.
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FIG. 1 is a photograph showing the effect of the microbial preparation of embodiment 6 on the control of tomato bacterial wilt; wherein A is the disease condition of tomato bacterial wilt in a blank control group, and B is the disease condition of tomato bacterial wilt in a microbial preparation group.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
Embodiment 1:
the embodiment provides Paenibacillus jimila W51, and the screening method of the strain is as follows:
paenibacillus jimirabilis W51 (CGMCC number 16639) is separated from tomato rhizosphere soil in wharf town of Huaian city, Jiangsu province. Cutting tomato root into 1cm segments, soaking in 1% sodium hypochlorite solution for 5 min, soaking in 75% alcohol for 90 s, and washing with sterile water for 3 times (applying 50 μ L of the last washing solution onto YGPA solid culture medium for culture, and considering surface sterilization and sterility if aseptic growth occurs after 48 h). 5 g of the sterilized sample was placed in a mortar, and 45 ml of a sterile 0.85% NaCl solution was added, and the tissue was macerated, ground, and diluted with a sterile 0.85% NaCl solution in a gradient manner. Get 10-1、10 -2 、10 -350 μ L of each of the three dilutions was applied to YGPA solid plate medium and incubated at 32 ℃ for 36 h. The largest single colony was selected and inoculated onto fresh YGPA solid plate medium, and the purified strain was obtained by repeated transfer. The purified strain was stored in an ultra-low temperature refrigerator at-80 ℃ with 50% glycerol.
The identification method comprises the following steps: the strain is identified by morphological characteristics, physiological and biochemical experiments and 16S rDNA sequence analysis
Morphological characteristics: gram-positive bacteria, rod-shaped, spore-producing, round colony, milky white, glossy and smooth surface.
16S rRNA Gene amplification and sequence analysis Paenibacillus jimmalaensis W51 was cultured in YGPB medium at 32 ℃ to log phase, centrifuged at 13000R/min for 10 min to collect the cells, DNA of strain W51 was extracted according to the protocol of bacterial DNA extraction kit (Promega, USA), and the extracted DNA product was used as a template for amplification by 27F and 1492R primers. The primer sequence is as follows:
27F：AGAGTTTGATCCTGGCTCAG
1492R：GGTTACCTTGTTACGACTT
A16S rDNA gene fragment was amplified from the genomic DNA. The PCR product is sent to Shanghai Meiji biological medicine science and technology limited company for sequencing. The sequencing results were as follows:
CGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCAATTCCGACTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGACCGGCTTTTCTAGGATTGGCTCCAcctCGcggcTTCGCTTCCCGTTGTACCGGCCATTGTAGTACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTGCTTAGAGTGCCCAGCTTGACCTGCTGGCAACTAAGCATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGAAAGGTCTATCTCTAGACCGGTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATACTCCACTGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATGTGTTAACTTCGGCACCAAGGGTATCGAAACCCCTAACACCTAGCATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGCCCAGAGAGTCGCCTTCGCCACTGGTGTTCCTCCACATCTCTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTCAAGCTCTCCAGTTTCCAGTGCGACCCGAAGTTGAGCCTCGGGATTAAACACCAGACTTAAAGAGCCGCCTGCGCGCGCTTTACGCCCAATAATTCCGGACAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTTCTCAGGTACCGTCACTCttgTAGCAGTTACTCTACAAGACGTTCTTCCCTGGCAACAGAGCTTTACGATCCGAAAACCTTCATCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATGCGCCGCAGGCCCATCCACAAGTGACAGATTGCTCCGCCTTTCCTCCTTCTCCCATGCAGGAAAAGGATGTATCGGGTATTAGCTACCGTTTCCGGTAGTTATCCCTGTCTTGTGGGCAGGTTGCCTACGTGTTACTCA
homology comparison of the determined 16S rRNA gene sequences by BLAST software, results are shown in Table 1, strain W51 and Paenibacillus jimirabilis reported in GenBankP. jamilae(KCTC 13919) has a closest relationship and homology of 99.65%, and therefore, the strain was identified as Paenibacillus jimmalaensis: (C.jimmatis.) (Paenibacillus jamilae）。
TABLE 1W 51 sequencing alignment results
| Bacterial strains | Most similar strains and accession numbers | Degree of similarity (%) |
| W51 | Paenibacillus jamilae KCTC13919 | 99.65 |
The Paenibacillus jimirabilis W51 is classified and namedPaenibacillus jamilaeAnd the strain is preserved in China general microbiological culture Collection center (CGMCC) in 26 months and 10 months in 2018, the preservation address is No. 3 of the institute of microbiology of the national academy of sciences, No. 3 of the Ministry of Naja, Beijing, and the preservation number is CGMCC number 16639.
Embodiment 2: determination of effective adsorption efficiency of illite adsorbent on W51
The Paenibacillus jiemarginians W51 (CGMCC number 16639) strain is inoculated into YPGB culture solution at 32 ℃, after shaking culture at 200 rpm for 12 h, the OD value of the Paenibacillus jiemarginians at 600 nm is measured by sampling in an ultraclean bench every 2 h, when the OD value is 0.8, the culture is finished, and the strain solution is used as seed strain solution. Mixing the W51 seed bacterial liquid in a ratio of 1:100 into PDB liquid culture medium, 32 ℃, 200 rpm oscillation culture 32 h after, 4000 rpm centrifugation 30min, abandon the supernatant, precipitate with sterile water heavy suspension and make its 600 nm absorbance value of 0.8. The adsorption effect of illite at various ratios on W51 cells was measured by sucrose density gradient method. Adding 4% illite powder (the granularity is less than 2 mu m, the natural whiteness is greater than 85%, and the particle size is provided by a key laboratory of probiotic preparation in Jiangsu province) into W51 bacterial suspension, carrying out shaking culture at 32 ℃ and 200 rpm in a gas bath shaker for 0.5 h, taking the mixed solution, slowly adding 60% of sucrose solution to the bottom of the mixed solution, standing for 2 h, precipitating thalli adsorbed on the illite to the bottom of the sucrose solution due to the fact that the thalli are large in mass, keeping the thalli not adsorbed on the upper layer of the sucrose solution, sucking the upper layer liquid to dilute and coat a flat plate, counting the number of single colonies after culturing for 12 h at 32 ℃, and calculating the adsorption rate C as follows:
wherein: m- -single colony number of the mixture solution on the upper layer of the sucrose solution after adding the adsorbent
M- -number of single colonies of bacterial suspension on the upper layer of sucrose solution without adding adsorbent
The adsorption rate of W51 was calculated to be 90.39% for 4% illite.
Embodiment 3: preparation of Paenibacillus jimila W51 microbial preparation
The Paenibacillus jiemarginians W51 (CGMCC number 16639) strain is inoculated into YPGB culture solution at 32 ℃, after shaking culture at 200 rpm for 12 h, the OD value of the Paenibacillus jiemarginians at 600 nm is measured by sampling in an ultraclean bench every 2 h, when the OD value is 0.8, the culture is finished, and the strain solution is used as seed strain solution. Inoculating seed bacteria liquid into PDB fermentation culture liquid at volume ratio of 1:100, fermenting at 32 deg.C and 210 rpm for 28 h, centrifuging at 4500 rpm for 30min, diluting the precipitate with sterile water to make bacterial suspension with OD value of 600 nm of 0.8, and adding sterilized illite powder (with particle size of 4%<2μm, natural whiteness>85% provided by key laboratory of probiotic preparation in Jiangsu province), culturing at 32 deg.C and 210 rpm for 0.5 hr under shaking to obtain microbial preparation with total viable bacteria concentration of 1 × 108 ~1×109 CFU/mL。
Embodiment 4: preparation of Paenibacillus jimila W51 microbial preparation
Inoculating Paenibacillus jimmalaensis W51 (CGMCC number 16639) strain into YPGB culture solution at 32 deg.C, shaking at 220 rpm for 10 h, sampling every 2 h in an ultraclean bench, measuring OD value at 600 nm, and culturing when OD value is 0.8 to obtain seed bacterial liquid. Inoculating seed bacteria liquid into PDB fermentation culture liquid at a volume ratio of 1:100, fermenting at 32 deg.C for 32 hr at 200 rpm, centrifuging at 5500 rpm for 20 min, diluting the precipitate with sterile water to obtain bacterial suspension with OD value of 600 nm of 1.0, and adding sterilized illite powder (with particle size of 4%<2μm, natural whiteness>85%), 32 ℃ and 200 rpm shaking culture for 1.0 h to obtain the microbial preparation, wherein the total viable bacteria concentration in the finished microbial preparation is 1 multiplied by 108 ~1×109 CFU/mL。
Embodiment 5: greenhouse test Paenibacillus jimela W51 microbial preparation control effect on tomato bacterial wilt (2018.04-2018.12)
A place: huaiyin institute of Industrial and scientific colleges greenhouse
Greenhouse pot experiment: the method comprises the steps of washing tomato seeds (the variety is Shanghai cooperative 903) twice with sterile water, washing the tomato seeds with 75% alcohol for two minutes, sterilizing the tomato seeds, washing the tomato seeds with the sterile water for three times repeatedly, culturing the sterilized tomato seeds in a culture dish to germinate, selecting the germinated seeds after 72 hours, sowing the germinated seeds into a hole basin, selecting seedlings with consistent growth vigor when the tomato seedlings grow to 5-6 main leaves, transferring the seedlings into a large basin for culturing, dividing experiments into a blank control treatment group and a microbial preparation treatment group, wherein each treatment group consists of 36 tomato seedlings, and repeating the experiments for three times. After 30 days, 15 mL of W51 microbial preparation was poured into the roots of the tomato seedlings in the microbial preparation treatment group, 15 mL of sterile water was poured into the blank control group, and bacterial wilt germs (concentration of 1X 10) were inoculated one week later7 CFU/mL, inoculum size 30 mL), greenhouse test conditions: and (5) naturally illuminating at 35 ℃. 7 d later, the disease condition of the tomato bacterial wilt is continuously observed and investigated for 15 days.
According to the tomato bacterial wilt resistance identification technical regulation (NY/T1858.4-2010), the research on the onset of the tomato bacterial wilt is combined with the actual situation of the experiment, and the analysis test data is recorded.
Grade 0, no symptoms;
grade 1, 1 leaf withered;
grade 2, 2-3 leaves withered;
grade 3, except 2-3 leaves at the bottom, the other leaves wither;
4, withering the whole leaf;
the disease level of each strain is recorded and investigated, and a disease index is calculated.
Disease Index (DI) = ∑ (disease progression × number of diseased plants)/(highest disease progression × total number of plants) × 100%
Biocontrol effect = (control group disease severity-treatment group disease severity)/control group disease severity × 100%
Note: the disease index and control effect of different treatments were calculated and differential significance analysis and multiple comparisons were performed using IBM SPSS Statistics 23 data analysis software.
The disease onset in the control and experimental groups after 15 days of microbial preparation treatment is shown in Table 1.
TABLE 1 biocontrol effect of microbial agents on tomato bacterial wilt
The result shows that the W51 microbial agent has better biological control effect on the tomato bacterial wilt under the greenhouse condition. The tomato seedlings treated by the W51 microbial preparation have the bacterial wilt disease index of 17.62 percent, and the biocontrol efficiency is 79.35 percent relative to 85.33 percent of the control group.
Embodiment 6: greenhouse test Paenibacillus jimela W51 microbial preparation control effect on tomato bacterial wilt (2019.02-2019.07)
A place: huaiyin institute of Industrial and scientific colleges greenhouse
Greenhouse pot experiment: the method comprises the steps of washing tomato seeds (the variety is Shanghai cooperative 903) twice with sterile water, washing the tomato seeds with 75% alcohol for two minutes, sterilizing the tomato seeds, washing the tomato seeds with the sterile water for three times repeatedly, culturing the sterilized tomato seeds in a culture dish to germinate, selecting the germinated seeds after 72 hours, sowing the germinated seeds into a hole basin, selecting seedlings with consistent growth vigor when the tomato seedlings grow to 5-6 main leaves, transferring the seedlings into a large basin for culturing, dividing experiments into a blank control treatment group and a microbial preparation treatment group, wherein each treatment group consists of 24 tomato seedlings, and repeating the experiments for three times. After 45 days, 10 mL of W51 microbial agent is filled into the roots of the tomato seedlings of the microbial agent treatment group, 10 mL of sterilized water is filled into the blank control group, and after one weekInoculating bacterial wilt bacteria (concentration of 1X 10) 7CFU/mL, inoculum size 20 mL), greenhouse test conditions: and (5) naturally illuminating at 35 ℃. After 5 d, the disease of the tomato bacterial wilt is continuously observed and investigated for 15 days.
According to the tomato bacterial wilt resistance identification technical regulation (NY/T1858.4-2010), the research on the onset of the tomato bacterial wilt is combined with the actual situation of the experiment, and the analysis test data is recorded.
Grade 0, no symptoms;
grade 1, 1 leaf withered;
grade 2, 2-3 leaves withered;
grade 3, except 2-3 leaves at the bottom, the other leaves wither;
4, withering the whole leaf;
the disease level of each strain is recorded and investigated, and a disease index is calculated.
Disease Index (DI) = ∑ (disease progression × number of diseased plants)/(highest disease progression × total number of plants) × 100%
Biocontrol effect = (control group disease severity-treatment group disease severity)/control group disease severity × 100%
Note: the disease index and control effect of different treatments were calculated and differential significance analysis and multiple comparisons were performed using IBM SPSS Statistics 23 data analysis software.
The disease of the control group and the experimental group after 15 days of the microbial preparation treatment is shown in fig. 1 and table 2.
TABLE 2 biocontrol effect of microbial agents on tomato bacterial wilt
The result shows that the W51 microbial agent has better biological control effect on the tomato bacterial wilt under the greenhouse condition. The tomato seedlings treated by the W51 microbial preparation have the bacterial wilt disease index of 15.83 percent, and the biocontrol efficiency is 78.58 percent relative to 73.90 percent of a control group.
In conclusion, the microbial preparation adopted by the invention has good effect, can effectively prevent and treat the tomato bacterial wilt, and has wide market application prospect.
The above embodiments are merely illustrative of the technical concepts and features of the present invention, and the purpose of the embodiments is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Sequence listing
<110> Huaiyin institute of Industrial and research
<120> method for controlling tomato bacterial wilt
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1304
<212> DNA
<213> Paenibacillus jie Mira (2 Ambystoma laterale x Ambystoma jeffersonanum)
<400> 1
cgggcggtgt gtacaagacc cgggaacgta ttcaccgcgg catgctgatc cgcgattact 60
agcaattccg acttcatgta ggcgagttgc agcctacaat ccgaactgag accggctttt 120
ctaggattgg ctccacctcg cggcttcgct tcccgttgta ccggccattg tagtacgtgt 180
gtagcccagg tcataagggg catgatgatt tgacgtcatc cccaccttcc tccggtttgt 240
caccggcagt ctgcttagag tgcccagctt gacctgctgg caactaagca taagggttgc 300
gctcgttgcg ggacttaacc caacatctca cgacacgagc tgacgacaac catgcaccac 360
ctgtctcctc tgtcccgaag gaaaggtcta tctctagacc ggtcagaggg atgtcaagac 420
ctggtaaggt tcttcgcgtt gcttcgaatt aaaccacata ctccactgct tgtgcgggtc 480
cccgtcaatt cctttgagtt tcagtcttgc gaccgtactc cccaggcgga atgcttaatg 540
tgttaacttc ggcaccaagg gtatcgaaac ccctaacacc tagcattcat cgtttacggc 600
gtggactacc agggtatcta atcctgtttg ctccccacgc tttcgcgcct cagcgtcagt 660
tacagcccag agagtcgcct tcgccactgg tgttcctcca catctctacg catttcaccg 720
ctacacgtgg aattccactc tcctcttctg cactcaagct ctccagtttc cagtgcgacc 780
cgaagttgag cctcgggatt aaacaccaga cttaaagagc cgcctgcgcg cgctttacgc 840
ccaataattc cggacaacgc ttgcccccta cgtattaccg cggctgctgg cacgtagtta 900
gccggggctt tcttctcagg taccgtcact cttgtagcag ttactctaca agacgttctt 960
ccctggcaac agagctttac gatccgaaaa ccttcatcac tcacgcggcg ttgctccgtc 1020
aggctttcgc ccattgcgga agattcccta ctgctgcctc ccgtaggagt ctgggccgtg 1080
tctcagtccc agtgtggccg atcaccctct caggtcggct acgcatcgtc gccttggtag 1140
gcctttaccc caccaactag ctaatgcgcc gcaggcccat ccacaagtga cagattgctc 1200
cgcctttcct ccttctccca tgcaggaaaa ggatgtatcg ggtattagct accgtttccg 1260
gtagttatcc ctgtcttgtg ggcaggttgc ctacgtgtta ctca 1304
Claims (5)
1. A method for preventing and treating tomato bacterial wilt is characterized in that a microbial preparation is used for carrying out root irrigation treatment on tomatoes; the microbial preparation comprises an adsorbent and Paenibacillus jimila (B.jimmatis) (R)Paenibacillus jamilae) W51, wherein the total viable bacteria concentration of Paenibacillus jimmalaensis W51 is 1 x 108 ~1×109 CFU/mL。
2. The method for controlling tomato bacterial wilt according to claim 1, wherein said Paenibacillus jie is deposited in China general microbiological culture Collection center (CGMCC) at 26.10.2018 with the deposition address of No. 3 of West Lu 1 of the Korean district in Beijing and the microbial research institute of China academy of sciences with the strain deposition number of CGMCC No. 16639.
3. The method for controlling tomato bacterial wilt according to claim 1, wherein the adsorbent is illite powder.
4. The method for controlling tomato bacterial wilt according to claim 1, wherein the concentration of the illite powder in the microbial preparation is 4%.
5. The method for controlling tomato bacterial wilt according to any one of claims 1 to 4, wherein the particle size of the illite powder is <2 μm, and the natural whiteness is > 85%.
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| CN109251879A (en) * | 2018-11-22 | 2019-01-22 | 青岛中达农业科技有限公司 | A kind of Jie meter La series bacillus fermentation process in high density |
| CN109266589A (en) * | 2018-11-22 | 2019-01-25 | 青岛中达农业科技有限公司 | Jie meter La series bacillus and its application of one plant of inhibition anthrax-bacilus and sickle-like bacteria |
| CN109868236A (en) * | 2019-01-31 | 2019-06-11 | 华南农业大学 | One plant of Jie meter La series bacillus, its tunning and preparation method and application |
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| CN105475363B (en) * | 2015-12-01 | 2018-01-16 | 中国农业科学院农业资源与农业区划研究所 | A kind of composite bacteria agent for promoting cucumber growth and its application |
| CN108410753B (en) * | 2018-02-06 | 2021-04-13 | 安徽师范大学 | Composite microbial inoculum for degrading straw, preparation method thereof and method for degrading straw |
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| CN109251879A (en) * | 2018-11-22 | 2019-01-22 | 青岛中达农业科技有限公司 | A kind of Jie meter La series bacillus fermentation process in high density |
| CN109266589A (en) * | 2018-11-22 | 2019-01-25 | 青岛中达农业科技有限公司 | Jie meter La series bacillus and its application of one plant of inhibition anthrax-bacilus and sickle-like bacteria |
| CN109868236A (en) * | 2019-01-31 | 2019-06-11 | 华南农业大学 | One plant of Jie meter La series bacillus, its tunning and preparation method and application |
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| CN114375989A (en) * | 2021-12-29 | 2022-04-22 | 淮阴工学院 | Application of Paenibacillus jimila W51 in prevention and treatment of strawberry and peach postharvest diseases and application method |
| CN114375989B (en) * | 2021-12-29 | 2024-03-26 | 淮阴工学院 | Application of Paenibacillus jemi W51 in prevention and treatment of postharvest diseases of strawberries and peaches and application method |
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| CN112825864B (en) | 2021-10-01 |
| CN112662591A (en) | 2021-04-16 |
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| CN112646752A (en) | 2021-04-13 |
| CN110669688A (en) | 2020-01-10 |
| CN112825864A (en) | 2021-05-25 |
| CN112640916B (en) | 2021-07-27 |
| CN110669688B (en) | 2021-07-02 |
| CN112760254B (en) | 2022-06-03 |
| CN112640916A (en) | 2021-04-13 |
| CN112646752B (en) | 2022-09-27 |
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