CN112760245B - Oil reservoir indigenous Dietzia and application thereof in oil exploitation - Google Patents
Oil reservoir indigenous Dietzia and application thereof in oil exploitation Download PDFInfo
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- CN112760245B CN112760245B CN201911075516.1A CN201911075516A CN112760245B CN 112760245 B CN112760245 B CN 112760245B CN 201911075516 A CN201911075516 A CN 201911075516A CN 112760245 B CN112760245 B CN 112760245B
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- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
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Abstract
The invention provides oil reservoir indigenous Dietzia bacteria and application thereof in oil exploitation. The invention firstly provides a deposit indigenous Dietzia (Dietzia cercidiphylli) with the preservation number of CGMCC No. 16105. The oil reservoir indigenous Dietzia can reduce the gas-liquid surface tension to 40.3mN/m and the oil-water interfacial tension to 6.7mN/m, form stable oil-in-water emulsion in a micro environment, and enable the oil washing efficiency of crude oil to reach 20.17%.
Description
Technical Field
The invention belongs to the technical field of oilfield microorganisms, and relates to oil reservoir indigenous Dietzia (Dietzia cercidiphylli) and application thereof in oil exploitation.
Background
Microbial oil recovery is one of tertiary oil recovery replacing technologies with great potential after water flooding, and has the characteristics of low cost, environmental friendliness, outstanding compound oil displacement mechanism and the like. The technical principle is mainly embodied in the following aspects:
(1) the microorganisms and metabolites thereof can change the physical properties of rocks, and effectively improve the porosity and permeability of an oil reservoir: a) organic acid in the metabolite can dissolve carbonate rock precipitate in pore throats, and the porosity and permeability of the rock are obviously increased; b) the attachment of microbial cells and exponential growth and propagation increase biomass, and can be used for plugging a macroporous throat and a high-permeability area and increasing swept volume; c) the extracellular macromolecular polymer can effectively improve the oil-water fluidity ratio in the displacement process, thereby enlarging swept volume and improving recovery efficiency; d) the metabolized organic alcohol, ketone and other solvents can dissolve the crude oil, improve the wettability of the rock, reduce the surface adhesion of the crude oil and increase the oil washing efficiency of water drive.
(2) The microorganism and the metabolite thereof improve the physical property and the quality of crude oil, reduce the viscosity of the crude oil, and enhance the fluidity of the crude oil: a) products such as biogas, organic acid, alcohol and the like are easily dissolved in the crude oil, so that a miscible effect is formed, the viscosity of the crude oil is reduced, the formation pressure is supplemented, and the development efficiency is improved. b) The anaerobic microorganism has the capability of degrading crude oil, and degrades large molecular heavy component alkane into small molecular light component, thereby improving the quality of the crude oil and reducing the viscosity of the crude oil. c) The organic solvent participates in the stabilization process, and the alcohol can be used as a cosurfactant to reduce the surface tension of gas and liquid and the interfacial tension of oil and water and promote the emulsification and viscosity reduction of crude oil.
The microbial oil recovery technology mainly comprises the steps of injecting exogenous bacteria after fermentation into a wellhead or adding an activating agent while injecting water to activate endogenous bacteria. The biochemical characteristics of the oil are utilized to directly (biodegradation) or indirectly (metabolite) act on the crude oil, and the physical and chemical properties of the crude oil are improved, so that the oil recovery rate is improved. At present, two technical modes of production increasing measures (blockage removal and profile control) and recovery increasing (single well stimulation and enhanced water drive) mainly exist in field application of the microbial oil recovery technology. However, the method is limited by the technical bottleneck problem of poor weather resistance of the oil reservoir (the harsh environment of the oil reservoir, such as temperature, pH value, mineralization and the like, greatly affect the physiological activity of the oil recovery functional bacteria), so that the field test effect is not ideal, and the technical popularization and application difficulty is high.
Disclosure of Invention
One object of the present invention is to provide a microorganism for oil exploitation.
According to a particular embodiment of the invention, the invention provides a strain of oil deposit indigenous Dietzia (Dietzia cercidiphylli), also designated ORF-0003, which has been deposited on a date of deposit: 13/7/2018; the preservation unit: china general microbiological culture Collection center (CGMCC); the address of the depository: the microbial research institute of the national academy of sciences No. 3, Xilu No.1, Beijing, Chaoyang, China, zip code: 100101; the preservation number is: CGMCC No. 16105; and (3) classification and naming: dietzia (Dietzia cercidiphylli) bacteria.
The invention also provides an oil reservoir indigenous Dietzia preparation which contains oil reservoir indigenous Dietzia with the preservation number of CGMCC No.16105 and is a solid or liquid bacterial preparation.
The oil reservoir indigenous Dietzia (Dietzia cercidiphylli) ORF-0003 (namely the strain with the preservation number of CGMCC No. 16105) has the characteristics of alkali resistance and mesophilic property, high mineralization resistance and better growth under low mineralization degree; has the capabilities of reducing gas/liquid surface tension, reducing oil/water interfacial tension, washing oil and improving petroleum recovery efficiency.
The invention also provides a biological oil-displacing agent, which is produced by fermenting the oil deposit indigenous Dietzia strain ORF-0003 or the oil deposit indigenous Dietzia strain preparation. Preferably, the biological oil-displacing agent is the oil deposit indigenous Dietzia bacteria or the oil deposit indigenous Dietzia bacteria preparation which is cultured in an LB culture medium to OD600Fermentation liquor with the fermentation time being more than or equal to 1.3.
The invention also provides a preparation method of the biological oil-displacing agent, which comprises the following steps: and (3) fermenting and culturing the oil reservoir indigenous Dietzia ORF-0003 or the oil reservoir indigenous Dietzia preparation in a culture medium to produce the biological oil-displacing agent. Preferably, the culture medium is LB culture solution, and is cultured to OD600≥1.3。
Therefore, the invention also provides the application of the oil reservoir indigenous Dietzia ORF-0003 or the oil reservoir indigenous Dietzia preparation or the biological oil displacement agent in oil exploitation.
The invention also provides application of the oil deposit indigenous Dietzia ORF-0003 or oil deposit indigenous Dietzia preparation or the biological oil displacement agent in oil displacement in an oil deposit rock core porous medium.
The invention also provides application of the oil reservoir indigenous Dietzia ORF-0003 or the oil reservoir indigenous Dietzia preparation or the biological oil displacement agent in reducing gas/liquid surface tension.
The invention also provides application of the oil deposit indigenous Dietzia ORF-0003 or the oil deposit indigenous Dietzia preparation or the biological oil displacement agent in reducing the interfacial tension of oil/water.
The invention also provides application of the oil deposit indigenous Dietzia ORF-0003 or the oil deposit indigenous Dietzia preparation or the biological oil displacement agent in emulsified crude oil.
The invention also provides application of the oil deposit indigenous Dietzia ORF-0003 or the oil deposit indigenous Dietzia preparation or the biological oil displacement agent in reducing the viscosity of crude oil.
In the application, the oil drop size of the crude oil after the crude oil is dispersed and emulsified by the oil deposit indigenous Dietzia cereidiphili ORF-0003 is preferably 8-22 μm.
The oil reservoir indigenous Dietzia cereidiphili ORF-0003 can reduce the gas-liquid surface tension to 40mN/m, reduce the oil-water interfacial tension to 6.7mN/m, and ensure that the oil washing efficiency reaches 20%.
In conclusion, the invention provides a strain of oil reservoir indigenous Dietzia (Dietzia cercidiphylli) ORF-0003, which has the characteristics of alkali resistance and mesophilic property, high mineralization resistance and better growth under low mineralization degree; has the capabilities of reducing gas/liquid surface tension, reducing oil/water interfacial tension, washing oil and improving petroleum recovery efficiency.
Drawings
FIG. 1 is a graph showing the growth curves of Dietzia cerealis ORF-0003 of indigenous Dietzia in example 2 of the present invention at different degrees of mineralization;
FIG. 2 is a graph showing the growth of Dietzia cereidiphilia ORF-0003 of indigenous Dietzia in example 3 of the present invention at different temperatures;
FIG. 3 is a graph showing the growth of Dietzia cereidiphilia ORF-0003 of indigenous Dietzia in example 4 of the present invention at different pH values;
FIG. 4 is a data graph showing the surface tension of the enriched solution of Dietzia cereidiphilia ORF-0003 and LB nutrient solution of indigenous Dietzia in oil reservoir as a function of time in example 5 of the present invention;
FIG. 5 is a data graph showing the interfacial tension of the enriched Dietzia cereidiphilia ORF-0003 liquid and LB nutrient solution of indigenous Dietzia in oil reservoir as a function of time in example 6 of the present invention;
FIG. 6 is a graph showing the oil washing effect of the enriched Dietzia cereuli ORF-0003 liquid and LB nutrient solution of indigenous Dietzia in oil reservoir of the present invention in example 7;
FIG. 7 is a graph showing the comparison of the oil droplet size of the enriched liquid of Dietzia cereidiphili ORF-0003 in example 8 of the present invention after crude oil emulsification with deionized water.
Microbial deposits for patent procedures:
the oil deposit indigenous Dietzia cereidiphylli ORF-0003 of the invention:
the preservation date is as follows: 13/07/2018;
the preservation unit: china general microbiological culture Collection center (CGMCC);
the address of the depository: the microbial research institute of the national academy of sciences No. 3, Xilu No.1, Beijing, Chaoyang, China, zip code: 100101;
the preservation number is: CGMCC No. 16105;
and (3) classification and naming: dietzia (Dietzia cercidiphylli) bacteria.
Detailed Description
The technical solutions of the present invention will be described in detail below in order to clearly understand the technical features, objects, and advantages of the present invention, but the present invention is not limited to the practical scope of the present invention.
Example 1 screening, isolation and cultivation of strains
Target reservoir depth 1420m, reservoir thickness 5m, reservoir permeability 450 × 10-3μm2The oil reservoir temperature is 50 ℃, the crude oil viscosity is 143mPa.s, the crude oil density is 0.8385g/cm3, the oil saturation is 73%, and the average freezing point is 24.3 ℃. Total microbial community DNA was extracted from target reservoir oil, water samples (using Invitrogen DNA extraction kit). Using 16S rDNA universal primer 27 f: 5'-AGAGTTTGATCCTGGCTCAG-3' (sequence shown as SEQ ID NO: 1), 1495 r: 5'-CTACGGCTACCTTGTTACGA-3' (sequence shown in SEQ ID NO: 2), and 16S rDNA PCR amplification experiments were performed. And (3) carrying out escherichia coli cloning and blue-white screening on the amplified PCR product (16S rDNA fragment), selecting bacterial colony points (blue) which are successfully cloned, sequencing, comparing sequencing results in a BLAST gene library, and judging the species relationship of the microorganisms. 96 indigenous strains were isolated from the oil-water sample, which contained Dietzia cereidiphili. And inoculating corresponding clone colonies for isolated culture according to the sequencing result. The culture mode adopts standard three-stage purification culture: solid colony culture in LB agar culture medium (10% tryptone, 5% yeast extract, 10% sodium chloride and 15% agar); ② 60mL LB liquid medium (ingredients: 1% peptone, 0.5% sodium chloride, 1% beef extract) liquid culture; ③ culturing 500mL of LB liquid to obtain the Dietzia cephriphili ORF-0003 of the oil reservoir origin Dietzia; dietzia cercidiphylli ORF-0003 belongs to the kingdom of bacteria, division of Actinomycetes, class of Actinomycetes, order of Corynebacteriales, family of Diaziaceae, genus Dietzia, and is a gram-positive, aerobic and facultative aerobic bacterium. The oil reservoir indigenous Dietzia Sercidiphylli ORF-0003 of the invention has been deposited. The preservation date is as follows: 13/07/2018; the preservation unit: china general microbiological culture Collection center (CGMCC); the address of the depository: the microbial research institute of the national academy of sciences No. 3, Xilu No.1, Beijing, Chaoyang, China, zip code: 100101; the preservation number is: CGMCC No. 16105; and (3) classification and naming: dietzia (Dietzia cercidiphylli) bacteria.
Example 2 evaluation experiment of mineralization degree and weather resistance
Preparation of the oil reservoir indigenous Dietzia bacteria Dietzia c of the inventionenrichment of ercidiphylli ORF-0003. 1. Spreading Dietzia cercidiphylli in LB agar medium (10% tryptone, 5% yeast extract, 10% sodium chloride, 15% agar), and standing at 30 deg.C for 24-48 hr; 2. inoculating single colony point into 60mL LB nutrient solution (10% tryptone, 5% yeast extract, 10% sodium chloride), culturing in a shaker at 30 deg.C and 150rpm (fully contacting with oxygen) for 16-18 hr, and culturing at OD600Not less than 1.3. Subsequently, 500. mu.L of the bacterial suspension was added to inorganic salt media having mineralization degrees of 8700g/L,11300g/L and 57300g/L, respectively, and cultured at 30 ℃ and 150rpm (sufficiently exposed to oxygen) for 28 hours. By OD600The detection of (2) shows the growth of the strain.
FIG. 1 shows the growth curves of Dietzia cereidiphilia ORF-0003 of the indigenous Dietzia genus in this example at different degrees of mineralization. This result demonstrates that the Dietzia cereidiphilia ORF-0003, the oil reservoir origin of the invention, is resistant to hypersalinity and grows better at low salinity.
Example 3 evaluation test of temperature weather resistance
The enrichment liquid of the oil deposit indigenous Dietzia Sercidiphylli ORF-0003 is prepared. 1. Spreading Dietzia cercidiphylli in LB agar medium (10% tryptone, 5% yeast extract, 10% sodium chloride, 15% agar), and standing at 30 deg.C for 24-48 hr; 2. inoculating single colony point into 60mL LB nutrient solution (10% tryptone, 5% yeast extract, 10% sodium chloride), culturing in a shaker at 30 deg.C and 150rpm (fully contacting with oxygen) for 16-18 hr, and culturing at OD600Not less than 1.3. Subsequently, 500. mu.L of the cell suspension was inoculated into 500mL of LB medium solution, and cultured for 36 hours in a shaker at 50 ℃, 60 ℃, 70 ℃ and 150rpm (sufficient contact with oxygen). By OD600The detection of (2) shows the growth of the strain.
FIG. 2 shows the growth curves of Dietzia cereidiphilia ORF-0003 of the indigenous Dietzia genus in this example at different temperatures. This result demonstrates that the Dietzia cereidiphilia ORF-0003, the origin of the oil reservoir, can grow in medium and high temperature oil reservoirs.
Example 4 evaluation test of pH weather resistance
The enrichment liquid of the oil deposit indigenous Dietzia Sercidiphylli ORF-0003 is prepared. 1. Spreading Dietzia cercidiphylli in LB agar medium (10% tryptone, 5% yeast extract, 10% sodium chloride, 15% agar), and standing at 30 deg.C for 24-48 hr; 2. inoculating single colony point into 60mL LB nutrient solution (10% tryptone, 5% yeast extract, 10% sodium chloride), culturing in a shaker at 30 deg.C and 150rpm (fully contacting with oxygen) for 16-18 hr, and culturing at OD600Not less than 1.3. Subsequently, 500. mu.L of the inoculum was inoculated into 500mL of LB nutrient solutions having pH values of 3, 5, 7, 9 and 11, respectively. The cells were incubated at 30 ℃ and 150rpm (sufficient oxygen exposure) for 36 hours on a shaker. By OD600The detection of (2) shows the growth of the strain.
FIG. 3 shows the growth curves of Dietzia cereidiphilia ORF-0003 of indigenous Dietzia in this example at different pH values. This result demonstrates that the Dietzia cereidiphilia ORF-0003, the indigenous source of the oil reservoir of the present invention, is capable of growing in alkaline oil reservoirs.
Example 5 surface tension evaluation test
Preparing enrichment liquid of oil reservoir indigenous Dietzia Sercidiphylli ORF-0003, adopting standard three-stage purification culture, 1. carrying out coating culture on Dietzia Sercidiphylli in an LB agar medium (components: 10% tryptone, 5% yeast extract, 10% sodium chloride and 15% agar), and carrying out standing culture at 30 ℃ for 24-48 hours; 2. inoculating single colony point into 60mL LB nutrient solution (10% tryptone, 5% yeast extract, 10% sodium chloride), culturing in a shaker at 30 deg.C and 150rpm (fully contacting with oxygen) for 16-18 hr, and culturing at OD600Not less than 1.3; 3. extracting 500 μ L of the bacterial liquid, inoculating into 500mL LB nutrient solution, culturing in a shaker at 30 deg.C and 150rpm (sufficient contact with oxygen)Nourishing for 16-18 hr, OD600Not less than 1.3, and obtaining the enrichment solution of Dietzia cercidiphylli ORF-0003.
The gas/liquid surface tension of the enriched liquid of Dietzia cereidiphilia ORF-0003 of the oil reservoir of the present invention was evaluated using a surface tension tester (model: DCAT 21; origin: Germany).
FIG. 4 shows the surface tension data of the enriched liquid of Dietzia cereidiphilia ORF-0003 and LB nutrient solution of Dietzia origin in this example as a function of time.
The results showed that the gas/liquid surface tension of the enriched liquid of the oil reservoir indigenous Dietzia cereidiphylli ORF-0003 of the invention was 40.3mN/m (as shown in FIG. 4). As a comparative test, the LB nutrient solution had a gas/liquid surface tension of 70.0mN/m (as shown in FIG. 4) similar to the effect of pure water (72.7 mN/m). This result demonstrates that the oil reservoir indigenous Dietzia cereidiphili ORF-0003 of the present invention can significantly reduce gas/liquid surface tension.
Example 6 evaluation experiment of interfacial tension
The change in the interfacial tension of oil droplets in the bacterial solution was evaluated using a rotary drop interfacial tension tester (model: TEXAS-500; origin: USA) (the interfacial tension leveled off within 120 minutes). Oil reservoir original Dietzia cereidiphylli ORF-0003 enriched solution was prepared as in example 2. The results showed that the crude oil/oil reservoir indigenous Dietzia cereidiphili ORF-0003 enriched liquid interfacial tension was 6.7mN/m (as shown in FIG. 2). As a comparative test, the crude oil/LB nutrient solution interfacial tension was 14.92 mN/m.
FIG. 5 shows the data of interfacial tension of enriched liquid and LB nutrient solution of Dietzia cereidiphilia ORF-0003 of indigenous Dietzia in this example as a function of time. The result proves that the Dietzia cephriphili ORF-0003 can obviously reduce the oil/water interfacial tension compared with the common oil production functional strains.
Example 7 evaluation test of oil washing Effect
The enrichment liquid of the Dietzia cereidiphilii ORF-0003 of the oil reservoir origin is prepared by adopting a standard three-level methodPurifying and culturing, 1. carrying out coating culture on Dietzia cercidiphylli in LB agar medium (components: 10% tryptone, 5% yeast extract, 10% sodium chloride and 15% agar), and standing and culturing at 30 ℃ for 24-48 hours; 2. inoculating single colony point into 60mL LB nutrient solution (10% tryptone, 5% yeast extract, 10% sodium chloride), culturing in a shaker at 30 deg.C and 150rpm (fully contacting with oxygen) for 16-18 hr, and culturing at OD600Not less than 1.3; 3. collecting 500 μ L of the bacterial liquid, inoculating into 500mL LB nutrient solution, culturing in a shaker at 30 deg.C and 150rpm (fully contacting with oxygen) for 16-18 hr, OD600Not less than 1.3, and obtaining the enrichment solution of Dietzia cercidiphylli ORF-0003.
16g of saturated oil sand (Jilin Ingtai Sael plot area, crude oil density 0.84g/cm3) And 50ml of the inoculum was placed in pear-shaped flasks and mixed overnight on a shaker at 50 deg.C (the inoculum was isolated from a 50 deg.C reservoir in Jilin) at 150 rpm. In addition, LB medium and pure water were used instead of the bacterial solution to make 2 controls. The next day, the tube was taken out from the 50 ℃ shaker and covered with a graduated oil-washing tube. Placing in a 50 ℃ oven, taking out after 8h, observing that 0.48ml of crude oil cultured by the bacterial liquid is separated out, and the crude oil separation amount of the LB culture medium control and the pure water control can be ignored.
FIG. 6 shows the oil washing effect of the enriched liquid of Dietzia cereidiphilia ORF-0003 and LB nutrient solution of Dietzia in this example.
By calculation, oil in saturated oil sands: when sand is 1:7, 16g of oil sand contains 2g of oil. The crude oil density is 0.84g/ml, which is equivalent to 2.38ml of crude oil, and the oil washing efficiency is 20% of 0.48/2.38.
Example 8 evaluation experiment of emulsification Effect
Pouring the oil deposit indigenous Dietzia cereidiphylli ORF-0003 bacterial liquid and crude oil into a glass measuring cylinder according to the volume ratio of 1:1(10mL:10mL), placing the glass measuring cylinder in a microwave cleaning instrument (model: CE-7200A; production area: China), and carrying out microwave oscillation for 15 minutes under the power of 80 watts to fully mix the oil deposit indigenous Dietzia cereidiphylli ORF-0003 bacterial liquid and the crude oil.
After oscillation, the liquid is divided into three levels: the upper layer is crude oil; the middle layer is an emulsifying layer; the lower layer is bacterial liquid. The emulsion stability (change in volume of the emulsion layer within 120 hours) was evaluated, and the emulsion effect of Dietzia cereidiphilia ORF-0003, which is an indigenous source of the oil reservoir, on crude oil was analyzed by observing the liquid in the emulsion layer under an electron microscope (model: Nikon Ti-S; origin: Japan) (evaluation of emulsion mode and emulsion particle size).
The experimental result shows that the emulsification effect of the oil reservoir indigenous Dietzia cereidiphylli ORF-0003 on the crude oil is stable, after standing for 60 hours, the emulsification layer of the crude oil/oil reservoir indigenous Dietzia cereidiphylli ORF-0003 tends to be stable, and does not change until the experiment is finished (120 hours), and the volume of the emulsification layer reaches 0.4 mL. As a comparison test, the oil and the water are completely separated after the emulsion layers in the two experiments of crude oil/water and crude oil/culture medium are kept still for 4 hours. The result proves that the oil reservoir indigenous Dietzia cereidiphili ORF-0003 has stronger crude oil emulsifying capacity compared with the common oil extraction strains.
Through microscopic observation of crude oil/oil reservoir indigenous Dietzia series Cercidiphili ORF-0003 emulsion layer liquid, the oil reservoir indigenous Dietzia series Cercidiphili ORF-0003 cells are tightly attached to the periphery of crude oil molecules to form a bridge with an aqueous solution, and the oil-in-water dispersion emulsification effect is formed on the crude oil. Meanwhile, the dispersed oil droplets were evaluated for particle size (see fig. 7) to be smaller and more uniform than those in oil-water two-phase solutions. This result shows that the oil reservoir indigenous Dietzia cereidiphili ORF-0003 has stronger dispersing and emulsifying capacity on crude oil.
Claims (13)
1. Oil reservoir indigenous Dietzia (S. di.) (Dietzia cercidiphylli) The preservation number is CGMCC number 16105.
2. An oil reservoir indigenous Dietzia preparation contains oil reservoir indigenous Dietzia with the preservation number of CGMCC number 16105, which is a solid or liquid bacterium preparation.
3. A biological oil-displacing agent which is the oil reservoir indigenous Dietzia bacteria of claim 1 or the oil reservoir indigenous Dietzia bacteria preparation of claim 2 cultured to OD in LB culture medium600Fermentation liquor with the fermentation time being more than or equal to 1.3.
4. A method of preparing the biological oil-displacing agent of claim 3, comprising: fermenting and culturing the oil reservoir indigenous dietzia bacteria of claim 1 or the oil reservoir indigenous dietzia bacteria preparation of claim 2 in a culture medium to produce a biological oil-displacing agent; wherein the culture medium is LB culture solution and is cultured to OD600≥1.3。
5. Use of the oil reservoir indigenous dietzia bacteria of claim 1, or the oil reservoir indigenous dietzia bacteria preparation of claim 2, or the biological oil displacement agent of claim 3 in oil recovery.
6. The application of the oil deposit indigenous dietzia bacteria of claim 1, the oil deposit indigenous dietzia bacteria preparation of claim 2 or the biological oil displacement agent of claim 3 in oil displacement in an oil deposit core porous medium.
7. Use of the oil reservoir indigenous dietzia bacteria of claim 1 or the oil reservoir indigenous dietzia bacteria preparation of claim 2 or the biological oil displacement agent of claim 3 for reducing gas/liquid surface tension.
8. The use according to claim 7, wherein the oil reservoir indigenous dietzia bacteria having a collection number of CGMCC number 16105 is capable of reducing gas-liquid surface tension to 40 mN/m.
9. Use of the oil reservoir indigenous dietzia bacteria of claim 1 or the oil reservoir indigenous dietzia bacteria preparation of claim 2 or the biological oil displacement agent of claim 3 to reduce the oil/water interfacial tension.
10. The use according to claim 9, wherein the oil pool indigenous dietzia bacteria having a collection number of CGMCC number 16105 is capable of reducing the oil-water interfacial tension to 6.7 mN/m.
11. Use of the oil reservoir indigenous dietzia bacteria of claim 1, or the oil reservoir indigenous dietzia bacteria preparation of claim 2, or the biological oil displacement agent of claim 3 in emulsifying crude oil.
12. The use as claimed in claim 11, wherein the oil droplets with the preservation number of CGMCC number 16105 and the oil drop size of 8-22 μm after the crude oil is dispersed and emulsified by the strain of Dietzia indigenous to the oil reservoir.
13. Use of the oil reservoir indigenous dietzia bacteria of claim 1, or the oil reservoir indigenous dietzia bacteria preparation of claim 2, or the biological oil displacement agent of claim 3 for reducing the viscosity of crude oil.
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| CN108060101A (en) * | 2018-01-09 | 2018-05-22 | 广州市第二中学 | Dietzia maris W02-3a and its application in denitrogenation |
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| Identification of hydrocarbon-oxidizing Dietzia bacteria from petroleum reservoirs based on phenotypic properties and analysis of the 16S rRNA and gyrB genes;T. N. Nazina等;《Microbiology》;20150609;第84卷;377-388 * |
| 驱油微生物对原油和沥青质的降解及模拟驱替效果研究;高卉;《中国博士论文全文数据库（电子期刊）工程科技I辑》;20190215;B019-44 * |
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