CN112725382A - Method for producing hydrogen by using salvaged and collected water bloom blue algae - Google Patents
Method for producing hydrogen by using salvaged and collected water bloom blue algae Download PDFInfo
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- CN112725382A CN112725382A CN202011269703.6A CN202011269703A CN112725382A CN 112725382 A CN112725382 A CN 112725382A CN 202011269703 A CN202011269703 A CN 202011269703A CN 112725382 A CN112725382 A CN 112725382A
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- 239000001257 hydrogen Substances 0.000 title claims abstract description 43
- 229910052739 hydrogen Inorganic materials 0.000 title claims abstract description 43
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 29
- 241000195493 Cryptophyta Species 0.000 title abstract description 22
- 241000192710 Microcystis aeruginosa Species 0.000 title abstract description 19
- 108010020056 Hydrogenase Proteins 0.000 claims abstract description 6
- 241000192700 Cyanobacteria Species 0.000 claims description 41
- 241000192701 Microcystis Species 0.000 claims description 7
- 230000010355 oscillation Effects 0.000 claims description 4
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 abstract description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 4
- 239000001301 oxygen Substances 0.000 abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 229910052786 argon Inorganic materials 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract description 2
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 239000003337 fertilizer Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 241001061264 Astragalus Species 0.000 description 1
- 235000010110 Astragalus glycyphyllos Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229910000863 Ferronickel Inorganic materials 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 235000006533 astragalus Nutrition 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for producing hydrogen by using salvaged and collected water bloom blue algae, which comprises the steps of directly packaging the collected water bloom blue algae in a closed container, and oscillating for 6-48 h at the speed of 60-120 rpm/min to produce hydrogen. The method for producing hydrogen does not need to remove the influence of oxygen on the activity of the hydrogenase by argon and other modes; the water bloom blue algae does not need to be collected by centrifugation and other modes; the hydrogen production condition is simple, the post-treatment problem of the water bloom blue algae salvaged ashore is solved, and clean energy can be produced, so that the aim of changing waste into valuables is fulfilled.
Description
Technical Field
The invention belongs to the technical field of environmental protection and new energy, and particularly relates to a method for producing hydrogen by using salvaged and collected water-blooming cyanobacteria.
Background
Due to the influence of the non-treatment discharge of industrial wastewater and domestic sewage, the use of chemical fertilizers and pesticides and other human factors, the eutrophication phenomenon of the water body is increasingly serious, and large-area outbreak of cyanobacterial bloom is caused. The outbreak of the cyanobacterial bloom not only seriously pollutes drinking water and disturbs the daily life of people, but also leads the water body to lose the original efficacies of ecology, entertainment, sightseeing and the like. Therefore, the treatment of the blue algae in water bloom has been paid attention by governments all over the world and has been generally concerned by people.
The salvaging and collecting are still the main methods for removing the bloom-forming cyanobacteria in the water body at present, and are widely used in various large lakes in China, including Yunnan lake in Yunnan, Taihu lake in Jiangsu and nested lake in Anhui. In the peak season of bloom-forming cyanobacteria outbreak, the amount of the bloom-forming cyanobacteria salvaged and collected in the Taihu lake every day can reach more than thousand tons. How to timely and effectively carry out innocent treatment and resource utilization on the salvaged water-blooming cyanobacteria on the shore is the key for successfully salvaging and treating the lake water body by the water-blooming cyanobacteria. If the salvaged water bloom blue algae can not be effectively treated in a harmless way and recycled, the water body and the surrounding ecological environment can be affected due to rotting, smelling and algal toxin release; the nitrogen, phosphorus and algae toxins generated by decomposition can flow into the lake again through surface runoff or leaching due to rain wash, thereby causing secondary pollution of the water body.
At present, scholars at home and abroad develop resource utilization research on the water-blooming cyanobacteria salvaged ashore. For example, Japanese scholars produce algae organic fertilizer by using bloom-forming cyanobacteria, and the fertilizer efficiency of the algae organic fertilizer is superior to that of common chemical fertilizers. Wherein, the contents of nitrogen, phosphorus and potassium are all higher than plant organic fertilizers such as bean cakes, milk vetch and the like, and heavy metal elements harmful to crops and human bodies are not contained, so that the soil is not polluted after the fertilizer is used. Chinese scholars produce biogas by using bloom-forming cyanobacteria as an anaerobic fermentation substrate. Chinese patent CN 1970776A (algae biogas energy fermentation method and products thereof) and Chinese patent CN 101337758B (water bloom blue algae anaerobic fermentation method) propose to obtain biogas, biogas slurry, biogas residues and other products by using an anaerobic fermentation method, thereby realizing the purpose of resource utilization. How to more effectively treat and recycle the water bloom blue algae salvaged ashore still remains a problem to be solved urgently.
Disclosure of Invention
The invention aims to effectively solve the problems of treatment and resource utilization of the bloom-forming cyanobacteria, and provides a method for producing hydrogen by using salvaged and collected bloom-forming cyanobacteria.
The purpose of the invention is realized by the following technical scheme:
a method for producing hydrogen by using salvaged and collected water bloom blue algae is characterized in that the collected water bloom blue algae is directly packaged in a closed container and vibrated at the speed of 60-120 rpm/min for 6-48 h to produce hydrogen.
Preferably, the water bloom blue algae is obtained by fishing and collecting water bloom blue algae growing in nature.
Preferably, the bloom-forming cyanobacteria comprises microcystis, and the microcystis bloom-forming cyanobacteria contains ferronickel hydrogenase and has the potential of producing hydrogen.
Preferably, the microcystis is an aggregate, further preferably, the diameter of the aggregate is 30-100 μm, and 2-10 layers of cells on the periphery of the aggregate are dead cells. Microcystis aggregation is a strategy to resist environmental stress conditions, and the appearance of dead cells in aggregates indicates that bloom-forming cyanobacteria begins to consume oxygen more rapidly. Thus, the appearance of aggregates of dead cells is an important indicator of the entry of bloom-forming cyanobacteria into anaerobic environments and the production of hydrogen.
The oscillation is carried out under the conditions of illumination, light and dark period alternation or darkness, and further preferably, the oscillation is carried out under the conditions of darkness and the oscillation time is 48 hours. Under the dark condition, the water bloom blue algae in the sealed container can be quickly induced to enter an anaerobic environment through the respiration of cells, hydrogenase is activated, and hydrogen is produced.
Preferably, hydrogen is produced by shaking at a rate of 120rpm/min for 48h, most preferably at a rate of 120rpm/min under dark conditions for 48 h.
Compared with the prior art, the invention has the following advantages:
the invention not only achieves the efficiency of hydrogen production but also achieves a better level (up to 3L H)2Above/g Chl a) and does not require any treatment, including high temperature and pressure and additives, nor complex production equipment. Therefore, the invention has simple hydrogen production condition and low cost, and shows the distinct creativity.
The collected water bloom blue algae is directly packaged in a closed container to vibrate to produce hydrogen, the hydrogen is clean energy with high development value, the influence of oxygen on the activity of hydrogenase is not required to be removed in the whole hydrogen production process in the modes of argon injection and the like, and the water bloom blue algae is not required to be collected in the modes of centrifugation and the like; the condition for inducing the hydrogen production is simple, and the efficiency of producing the hydrogen by the bloom-forming cyanobacteria treated under the dark condition is highest. Therefore, the technical scheme of the invention is a simple, convenient, quick, economic and efficient method for producing hydrogen by utilizing the salvaged and collected water bloom blue algae.
Drawings
FIG. 1 is water-blooming cyanobacteria (left) and its aggregates (right) under natural growth conditions;
FIG. 2 is water bloom cyanobacteria under laboratory culture conditions;
FIG. 3 is a comparison graph of natural accumulated bloom-forming cyanobacteria and laboratory dispersed bloom-forming cyanobacteria under dark conditions;
wherein: (a) the accumulated amount of hydrogen in 48 hours; (b) the oxygen content above the closed container in 48 hours; (c) hydrogenase activity was found within 48 hours.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments.
The water-blooming cyanobacteria adopted in the following examples is the water-blooming cyanobacteria which is outbreaked in the red sand bay of the Wuxi Taihu lake in 2019 in 8-10 months, the dominant algal species is microcystis, and FIG. 1 shows the water-blooming cyanobacteria (left side) and the aggregates thereof (right side);
FIG. 2 shows bloom-forming cyanobacteria under laboratory culture conditions.
Example 1
Directly putting the water-blooming cyanobacteria (100mg Chl a) fished ashore into a 500mL closed transparent container, and placing the container in illumination (40 mu mol phosns m)-2s-1) Oscillating (120rpm/min) under the condition to induce hydrogen production, and detecting the yield of the produced hydrogen every 6 hours.
200 μ l of gas was withdrawn from above in the closed vessel with a micro-syringe and a sample of the withdrawn gas was injected into a gas phase system equipped with a thermal conductivity detector (GC, 7890A, Agilent, USA) for hydrogen detection.
And (3) detection results: the hydrogen accumulation amount reaches 167 mu mol H at 48 hours2Per 100mg Chl a, as shown in FIG. 3.
Example 2
An experiment was conducted in the same manner as in example 1 except that the cyanobacteria bloom (100mg Chl a) was directly charged into a 500mL closed vessel and then alternately treated with a light-dark cycle (12/12 hours).
And (3) detection results: the hydrogen accumulation at 48 hours was 9568. mu. mol H2Per 100mg Chl a, as shown in FIG. 3.
Example 3
An experiment was conducted in the same manner as in example 1 except that the cyanobacteria bloom (100mg Chl a) was directly charged into a 500mL closed vessel and then treated in a completely dark condition.
And (3) detection results: the hydrogen accumulation at 48 hours was 13766. mu. mol H2Per 100mg Chl a, as shown in FIG. 3.
Example 3 works best with the maximum hydrogen production obtained by dark conditions, about 82 times the hydrogen production of light conditions and 1.5 times the hydrogen production of alternating light and dark conditions.
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (10)
1. The method for producing hydrogen by using the salvaged and collected bloom-forming cyanobacteria is characterized in that the collected bloom-forming cyanobacteria is directly packaged in a closed container and vibrated at the speed of 60-120 rpm/min for 6-48 h to produce hydrogen.
2. The method for producing hydrogen by using the salvaged and collected water-blooming cyanobacteria as claimed in claim 1, wherein the water-blooming cyanobacteria is obtained by salvaging and collecting water-blooming cyanobacteria growing in nature.
3. The method for producing hydrogen by using the salvaged and collected water-blooming cyanobacteria as claimed in claim 1, wherein the water-blooming cyanobacteria comprises microcystis.
4. The method for producing hydrogen by using the salvaged and collected water-blooming cyanobacteria as claimed in claim 3, wherein the microcystis is an aggregate.
5. The method for producing hydrogen by using the salvaged and collected water-blooming cyanobacteria as claimed in claim 4, wherein the diameter of the aggregate is 30-100 μm.
6. The method for producing hydrogen by using the salvaged and collected water-blooming cyanobacteria as claimed in claim 4, wherein 2-10 layers of cells on the periphery of the aggregate are dead cells.
7. The method for producing hydrogen by using the salvaged and collected cyanobacteria bloom as claimed in claim 1, wherein the cyanobacteria bloom contains hydrogenase.
8. The method for producing hydrogen by using the salvaged and collected water-blooming cyanobacteria as claimed in claim 1, wherein the shaking is performed under light, alternating light and dark periods or dark conditions.
9. The method for producing hydrogen by using the salvaged and collected water-blooming cyanobacteria as claimed in claim 8, wherein the shaking is performed under dark conditions.
10. The method for producing hydrogen by using the salvaged and collected water-blooming cyanobacteria as claimed in claim 9, wherein the oscillation time is 48 h.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011269703.6A CN112725382A (en) | 2020-11-13 | 2020-11-13 | Method for producing hydrogen by using salvaged and collected water bloom blue algae |
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| CN202011269703.6A CN112725382A (en) | 2020-11-13 | 2020-11-13 | Method for producing hydrogen by using salvaged and collected water bloom blue algae |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070269874A1 (en) * | 2003-10-01 | 2007-11-22 | Midwest Research Institute | Multi-Stage Microbial System for Continous Hydrogen Production |
| CN102618585A (en) * | 2012-03-28 | 2012-08-01 | 上海师范大学 | Method for producing hydrogen according to anaerobic fermentation of bloom-forming cyanobacteria |
| CN107267395A (en) * | 2017-07-13 | 2017-10-20 | 浙江大学 | A kind of method that micro-algae culture medium and culture microalgae produce hydrogen |
| CN110213964A (en) * | 2016-11-22 | 2019-09-06 | 纽泰克温图斯公司 | It is prepared using the individuation cell biological of closing minicell culture systems |
-
2020
- 2020-11-13 CN CN202011269703.6A patent/CN112725382A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070269874A1 (en) * | 2003-10-01 | 2007-11-22 | Midwest Research Institute | Multi-Stage Microbial System for Continous Hydrogen Production |
| CN102618585A (en) * | 2012-03-28 | 2012-08-01 | 上海师范大学 | Method for producing hydrogen according to anaerobic fermentation of bloom-forming cyanobacteria |
| CN110213964A (en) * | 2016-11-22 | 2019-09-06 | 纽泰克温图斯公司 | It is prepared using the individuation cell biological of closing minicell culture systems |
| CN107267395A (en) * | 2017-07-13 | 2017-10-20 | 浙江大学 | A kind of method that micro-algae culture medium and culture microalgae produce hydrogen |
Non-Patent Citations (2)
| Title |
|---|
| LANZHEN WEI ET AL.: "A simple approach for the efficient production of hydrogen from Taihu Lake Microcystis spp. blooms", 《BIORESOURCE TECHNOLOGY》 * |
| 任文伟等: "《蓝藻水华生态防治新理论与新技术》", 31 December 2012, 上海科技教育出版社 * |
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Application publication date: 20210430 |