CN112725249B - Growth-promoting compound microbial agent and application thereof - Google Patents
Growth-promoting compound microbial agent and application thereof Download PDFInfo
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Abstract
The invention relates to a growth-promoting compound microbial agent and application thereof, wherein the microbial agent comprises Bacillus belgii YZS-M03, small strange bacteria BQB-B04 and Bacillus altivelis ZLB-C01. The component strains of the growth-promoting compound microbial agent have strong phosphate-solubilizing potassium-solubilizing nitrogen-fixing effects and can be colonized in plants. The growth-promoting compound microbial agent can improve the chlorophyll content of plants, improve the activity of plant enzymes and promote the growth and development of the plants.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and relates to a growth-promoting compound microbial agent and application thereof.
Background
The problems of weak permeability, poor water and fertilizer retention, poor soil fertility and the like in the current soil seriously affect the sustainable development of agriculture. Therefore, improving soil, increasing soil productivity, improving soil ecological environment, and increasing crop yield and quality have become the key points of current agricultural work. Microorganisms play a very important role in the input and output of substances and energy in soil, and are an important link in a substance circulation chain. It can activate organic and inorganic nutrients in soil, decompose organic matter, release nutrients and increase the effectiveness of nutrients. During the propagation and metabolism of the microorganisms, the microorganisms can degrade residual fertilizers, organic pesticides, heavy metals and other pollutants in the soil, decompose, convert, fix and transfer the pollutants in the physicochemical reaction of the microorganisms, and decompose the pollutants into low-harmful or even harmless substances, thereby reducing the degree of soil pollution. In addition, microorganisms in the soil, such as antibiotic microorganisms, can secrete antibiotics to inhibit the propagation of pathogenic microorganisms, so that the harm of soil-borne microorganisms in the soil to crops can be prevented, reduced and the quality and yield of the crops can be improved. The microbial agent is a solid microbial agent preparation which is obtained by industrially producing microbes to obtain solid bacterial powder and then adsorbing the solid bacterial powder by a certain carrier; the microbial agent liquid preparation is prepared by mixing single microbial fermentation liquor or multiple microbial fermentation liquor obtained after industrial fermentation according to a certain proportion.
Disclosure of Invention
The invention aims to provide a growth-promoting compound microbial agent and application thereof. The component strains of the growth-promoting compound microbial agent have strong phosphate-solubilizing potassium-solubilizing nitrogen-fixing effects and can be colonized in plants. The growth-promoting compound microbial agent can improve the chlorophyll content of plants, improve the activity of plant enzymes and promote the growth and development of the plants.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a growth-promoting compound microbial agent, which comprises Bacillus bleekeri YZS-M03, Microstranguria cruzi BQB-B04 and Bacillus altissima ZLB-C01.
The mass ratio of solid bacterial powder or fermentation liquor of the Bacillus belgii YZS-M03, the small strange bacteria BQB-B04 and the Bacillus altivelis ZLB-C01 in the growth-promoting compound microbial agent is (3-5): (3-6): (3-8).
The Bacillus belgii YZS-M03 was classified and named Bacillus belgii: (Bacillus velezensis) The microbial culture has been preserved in China general microbiological culture Collection center (CGMCC) at 16 months 10 and 2020, and the preservation address is as follows: china Beijing, the preservation number is: CGMCC No. 20900.
The aforementioned small stranguria Clahsira BQB-B04 is named as small stranguria Clahsira (Clahsira)Advenella kashmirensis) The microbial culture has been preserved in China general microbiological culture Collection center at 2 months and 1 day 2021, with the preservation address: china Beijing, the preservation number is: CGMCC No. 21796.
The Bacillus altitudinis ZLB-C01 classified and named as Bacillus altitudinis (A)Bacillus altitudinis) The microbial culture has been preserved in China general microbiological culture Collection center (CGMCC) at 16 months 10 and 2020, and the preservation address is as follows: china Beijing, the preservation number is: CGMCC No. 20898.
The total viable bacteria concentration in the growth-promoting compound microbial agent is (5-8) multiplied by 108cfu/ml or (2-3). times.109cfu/g。
The growth-promoting compound microbial agent is a liquid preparation or a solid preparation.
The preparation method of the growth-promoting compound microbial agent solid preparation comprises the following steps: mixing solid fungus powder of Bacillus beiLeisi YZS-M03, small stranguria Kloshimi BQB-B04 and Bacillus altissilis ZLB-C01 according to mass ratio to prepare solid mixed fungus powder; and mixing a carrier and the solid mixed bacterium powder in a mass ratio of 100: 2-5 to obtain the growth-promoting compound microbial agent solid preparation, wherein the carrier is bran.
The preparation method of the growth-promoting compound microbial agent liquid preparation comprises the following steps: the bacillus beiLeisi YZS-M03, the small stranguria Kloshimi BQB-B04 and the bacillus altitudinis ZLB-C01 are obtained by mixing fermentation liquor according to the mass ratio of (3-5) to (3-6) to (3-8).
The invention provides application of the growth-promoting compound microbial agent in promoting plant growth.
The invention has the beneficial effects that:
the component strains of the growth-promoting compound microbial agent have strong phosphate-solubilizing potassium-solubilizing nitrogen-fixing effects and can be colonized in plants. The growth-promoting compound microbial agent can improve the chlorophyll content of plants, improve the activity of plant enzymes, and has an important promotion effect on the regulation of the growth and development of plants. The growth-promoting compound microbial agent is inoculated to plant seedlings through root irrigation, so that the chlorophyll content of leaves can be obviously improved, the contents of Catalase (CAT), Peroxidase (POD) and superoxide dismutase (SOD) are improved, and compared with a control, the difference is obvious; can promote the growth and development of plants and obviously improve the plant height. Therefore, the invention provides a compound microbial agent with strong growth promoting function for plants.
Drawings
FIG. 1 shows the change of SPAD value of rape leaves after being treated by the growth-promoting compound microbial agent liquid preparation 1.
FIG. 2 shows CAT activity changes of rape leaves after the growth-promoting compound microbial agent liquid preparation 1 is treated.
FIG. 3 shows the change of POD activity of rape leaves after the treatment of the growth-promoting complex microbial inoculant liquid preparation 1.
FIG. 4 shows the change of SOD activity of rape leaves after the treatment of the growth-promoting compound microbial inoculum liquid preparation 1.
FIG. 5 shows the effect of the growth-promoting complex microbial inoculum liquid preparation 1 on the growth of rape.
FIG. 6 shows the change of SPAD value of Shanghai green leaf after treatment with the growth-promoting complex microbial inoculum liquid preparation 2
FIG. 7 change in CAT activity of the leaves of Haematococcus japonicus after treatment with the growth-promoting complex microbial inoculant liquid preparation 2.
Fig. 8 change in POD activity of shanghai green leaves after treatment with growth-promoting complex microbial inoculant liquid preparation 2.
FIG. 9 shows the change of SOD activity of leaves of Shanghai green after treatment with the growth-promoting complex microbial inoculum liquid preparation 2.
FIG. 10 effect of growth promoting complex microbial inoculant liquid preparation 2 on growth of Shanghai green.
Detailed Description
The present invention is further illustrated by the following examples.
Strain material:
(1) bacillus belgii YZS-M03, which is classified and named as Bacillus belgii ((R))Bacillus velezensis) The microbial inoculum is preserved in China general microbiological culture Collection center at 16 months 10 and 2020, and the preservation number is as follows: CGMCC No.20900, address No. 3 of Xilu No. 1 of Beijing area facing Yang district. In the present invention, the bacillus beilesensis YZS-M03 is an endophytic bacterium isolated and purified from a plant, and the colony characteristics and cell morphology of the bacillus beilesensis YZS-M03 are as follows: after culturing on an NA plate for 24 hours, the formed colony is light yellow, matt, round, free of mobility, gram-positive, spore-containing and capsular-free; the colony diameter is 4-5 mm. The physiological and biochemical characteristics of the Bacillus belgii YZS-M03 are as follows: the catalase reaction is negative, the V.P test is negative, the MR test is negative, the glucose acidogenesis test is negative, the citrate test is negative, the nitrate reduction is positive, the starch hydrolysis is positive, the indole test is negative, the malonic acid test is negative, the H production is negative2Positive in S test. The 16S rDNA sequence of the Bacillus belgii is compared with the sequence in a GenBank database, and the result shows that YZS-M03 and the sequence in the GenBank database are differentBacillus velezensisOn the same branch, the 16S rDNA sequence andBacillus velezensisthe similarity of (MT 509947.1) reaches 99.86%. Combined with colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, the bacillus belgii is identified as bacillus (B)Bacillus velezensis）。
(2) Small stranguria keshmania BQB-B04, which is classified and named as small stranguria keshmania (A)Advenella kashmirensis），Has been preserved in China general microbiological culture Collection center (CGMCC) at 2021, 2 months and 1 days, and the preservation numbers are: CGMCC No.21796, address No. 3 of Xilu No. 1 of Beijing area facing Yang. In the invention, the small stranguria keliensis BQB-B04 is an endophytic bacterium obtained by separating and purifying from a plant, and the colony characteristics and the thallus form of the small stranguria keliensis BQB-B04 are as follows: after culturing on an NA plate for 24 hours, the formed colony is yellow-white, matt, round, slightly convex on the surface, opaque, neat in edge, gram-positive, spore-free and capsular; the diameter of the colony is 2-3 mm.
The physiological and biochemical characteristics of the endogenous phosphorus-dissolving potassium-dissolving nitrogen-fixing small strange bacteria BQB-B04 are as follows: catalase reaction is negative, V.P test is negative, MR test is positive, glucose acidogenesis test is negative, citrate test is positive, nitrate reduction is positive, starch hydrolysis is negative, indole test is negative, malonic acid test is positive, H production is positive2Positive in S test. The 16S rDNA sequence of the endogenous phosphorus-dissolving potassium-dissolving nitrogen-fixing small strange bacteria is compared with the sequence in a GenBank database, and the result shows that BQB-B04 and the sequence in the GenBank database are comparedAdvenella kashmirensisOn the same branch, the 16S rDNA sequence andAdvenella kashmirensis(KU 043380.1) was found to be 98.52% similar. Combined with colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, the strain is identified as small strange bacteria of the Klishmi: (Advenella kashmirensis）。
(3) Bacillus altitudinis ZLB-C01, and its classification name is Bacillus altitudinis (A), (B), (C01), and (B), and C) and B), and (B) a)Bacillus altitudinis) The microbial inoculum is preserved in China general microbiological culture Collection center at 16 months 10 and 2020, and the preservation number is as follows: CGMCC No.20898, address No. 3 of Xilu No. 1 of Beijing, Chaoyang. In the invention, the Geobacillus altivelis ZLB-C01 is an endophytic bacterium obtained by separating and purifying from a plant, and the colony characteristics and the thallus morphology of the Geobacillus altivelis ZLB-C01 are as follows: after 24h of culture on an NA plate, the formed colony is milky white, matt, round, free of fluidity, gram-positive and spore(ii) no capsule; the colony diameter is 4-5 mm. The physiological and biochemical characteristics of the Geobacillus altivelis ZLB-C01 are as follows: catalase reaction is negative, V.P test is positive, MR test is positive, glucose acidogenesis test is negative, citrate test is negative, nitrate reduction is positive, starch hydrolysis is negative, indole test is negative, malonic acid test is negative, H production is negative2Positive in S test. The 16S rDNA sequence of the Geobacillus altitudinis is compared with the sequence in a GenBank database, and the result shows that the ZLB-C01 and the GenBank databaseBacillus altitudinisOn the same branch, the 16S rDNA sequence andBacillus altitudinisthe similarity of (MW 474842.1) reaches 99.86%. Combining colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, and identifying the bacillus altitudinis: (Bacillus altitudinis）。
The invention provides a growth-promoting compound microbial agent, which comprises Bacillus bleekeri YZS-M03, small strange bacteria BQB-B04 and Bacillus altivelis ZLB-C01; the growth promoting compound microbial agent comprises Bacillus beijerinckii YZS-M03, Microstranguria keishii BQB-B04 and Bacillus altissimus ZLB-C01 in a mass ratio of (3-5) to (3-6) to (3-8); the total viable bacteria concentration in the growth-promoting compound microbial agent is (5-8) multiplied by 108cfu/ml or (2-3). times.109cfu/g。
In the present invention, the growth-promoting complex microbial agent is preferably a liquid preparation or a solid preparation. When the growth-promoting compound microbial agent is a solid preparation, the growth-promoting compound microbial agent comprises a carrier. In the invention, the carrier is bran. The total mass ratio of the bran to the Bacillus beiLeisi YZS-M03, the small stranguria Klishii BQB-B04 and the Bacillus altivelis ZLB-C01 is preferably 100 (2-5). In the invention, when the growth-promoting compound microbial agent is a solid preparation, solid bacterial powder is prepared by respectively carrying out freeze drying or spray drying on fermentation liquor of the bacillus beijerinckii YZS-M03, the small strange bacteria BQB-B04 and the bacillus plateau ZLB-C01, and then mixing the solid bacterial powder in proportion. In the invention, when the growth-promoting compound microbial agent is a liquid preparation, the growth-promoting compound microbial agent is obtained by mixing fermented fermentation liquor of Bacillus belgii YZS-M03, Microstranguria cruzi BQB-B04 and Bacillus altivelis ZLB-C01 in a mass ratio of (3-5) to (3-6) to (3-8).
In the present invention, the preparation method of the solid bacterial powder preferably comprises the following steps: activating the strain, then carrying out continuous two-stage seed liquid culture, inoculating into a fermentation culture medium for fermentation and concentration to obtain concentrated bacterial liquid, mixing the concentrated bacterial liquid of the small strange bacterium of the kashmir BQB-B04 with a protective agent (skimmed milk powder), and then carrying out freeze drying to obtain solid bacterial powder; and mixing the fermentation liquor of the bacillus beilesensis YZS-M03 and the bacillus altitudinis ZLB-C01 with light calcium carbonate, and then carrying out spray drying to obtain the solid bacterial powder.
In the invention, a strain is inoculated in an NB culture medium and cultured for 15-25 h to obtain a primary seed solution; the culture temperature is preferably 25-35 ℃, and the culture rotation speed is preferably 150-350 r.min-1。
After the first-stage seed liquid is obtained, the first-stage seed liquid is transferred to an NB liquid culture medium to be cultured for 10-20 h to obtain a second-stage seed liquid. In the invention, the inoculation amount of the primary seed liquid transfer is preferably 0.5-1.0% (V/V), the culture is preferably carried out in a fermentation tank, sterile air is introduced in the culture process, the DO (DO 40) is regulated by manually regulating the rotation speed and the air flow of the fermentation tank, the culture temperature is 25-35 ℃, and the rotation speed of the culture is 200-350 r.min-1The culture time is preferably 10-20 h. In the present invention, the OD of the secondary seed liquid600Preferably 0.6 to 1.0.
After the secondary seed liquid is obtained, the secondary seed liquid is inoculated into a fermentation culture medium for fermentation culture to obtain fermentation liquor. In the invention, the inoculation amount of the secondary seed liquid is 0.5-1.0% (V/V), and the tank pressure in the fermentation culture process is maintained at 0.05 MPa; sterile air is introduced in the culture process, the fermentation tank is aerated to manually adjust the rotating speed and the air flow rate to adjust DO (DO 40%), the culture temperature is 25-35 ℃, and the culture rotating speed is 200-350 r.min-1What is, what isThe culture time is preferably 15-35 h. In the present invention, the OD of the secondary seed liquid600Preferably 0.8 to 1.5.
After the zymocyte liquid is obtained, the zymocyte liquid is concentrated to obtain concentrated bacteria liquid. In the invention, the concentration multiple of the concentrated bacterial liquid is 5-20 times; in the concentration method, the fermentation bacteria liquid of the small stranguria keshmania BQB-B04 is subjected to high-speed centrifugation to obtain concentrated bacteria liquid; and the fermentation bacteria liquid of the Bacillus belgii YZS-M03 and the Bacillus altitudinis ZLB-C01 is filtered by a ceramic membrane to obtain concentrated bacteria liquid. The invention has no special limitation on the specific parameter setting of the concentration equipment, and the concentration of the multiple can be realized by adopting the conventional parameter setting.
After the zymophyte liquid is obtained, centrifuging the zymophyte liquid of the small strange bacterium of the kashmir BQB-B04 through a high-speed centrifuge to obtain concentrated bacteria liquid, adding a protective agent (skimmed milk powder) into the concentrated bacteria liquid, and then performing vacuum freeze drying to obtain solid bacteria powder, wherein the mass ratio of the concentrated liquid to the protective agent (skimmed milk powder) is 10:1, the vacuum freeze drying process is controlled to be performed under the condition that the concentrated liquid is cooled to-40 ℃ for 3-5 h, the concentrated liquid is vacuumized, hot water is introduced for controlling the temperature to-20 ℃, and the concentrated liquid is sublimated to remove water. And filtering the fermentation bacteria liquid of the Bacillus belgii YZS-M03 and the Bacillus altitudinis ZLB-C01 by a ceramic membrane to obtain concentrated bacteria liquid, adding light calcium carbonate into the concentrated bacteria liquid, and then performing spray drying to obtain solid bacteria powder, wherein the mass ratio of the concentrated liquid to the light calcium carbonate is 100: 2-5, the spray drying process is controlled by controlling the material inlet temperature to be 150-200 ℃, the material outlet temperature to be 80-85 ℃, and the material drying time in the tower to be 15-30S.
EXAMPLE 1 preparation of growth-promoting Complex microbial Agents
(1) Preparing bacterial liquid and solid bacterial powder of small strange bacteria BQB-B04 of the Kloshnel:
preparation of first-grade seed solution of Microstranguria keishii BQB-B04
Activating the strain BQB-B04 of small strange bacterium Klishimil, picking out single colony, inoculating in 150ml NB culture medium (sterilized at 121 deg.C for 30 min), sterilizing at 28 deg.C for 180 r.min-1Shaking and culturing for 20h under the condition to obtainFirst-stage seed liquid.
Preparation of second-grade seed solution of small stranguria Klishmi BQB-B04
Inoculating the first-grade seed liquid into a sterile seed tank, wherein the inoculation amount is 0.5-1.0% (V/V); the culture conditions were: adjusting DO (DO 40%) by manually adjusting the rotating speed and the air flow rate through ventilation, wherein the culture temperature is 25-35 ℃, and the rotating speed is 200-350 r.min-1The tank pressure is 0.05 MPa; the culture time is 10-15 h. OD of second-stage seed liquid600The value is 0.6-0.8, and the strain activity is strongest at the moment.
Preparation of fermentation liquor of small strangles closhmania BQB-B04:
inoculating the secondary seed liquid of the small strange bacteria BQB-B04 of the Klishmi into a sterile fermentation tank according to the inoculation amount of 0.5-1.0% (V/V), wherein the fermentation medium comprises the following components (per liter): 25-50 g of yeast extract and 12-20 g of maltose syrup. The culture conditions were: adjusting DO (DO 40%) by manually adjusting the rotating speed and the air flow rate through ventilation, wherein the culture temperature is 25-35 ℃, and the rotating speed is 200-350 r.min-1The tank pressure is 0.05 MPa; the culture time is 15-20 h, the pH is raised to 7.5-8.0 and the OD is increased600And (5) stopping fermentation when the value is 0.8-1.0 to obtain mature fermentation bacterial liquid.
Preparation of small strange bacteria BQB-B04 keshimei powder:
centrifuging the obtained fermented mature bacterial liquid by a high-speed centrifuge, adding a protective agent (skimmed milk powder), uniformly mixing, wherein the mass ratio of the concentrated bacterial liquid to the protective agent (skimmed milk powder) is 10:1 to obtain a solid-liquid mixture, and carrying out vacuum freeze drying under the following conditions: cooling for 3-5 h to-40 ℃ for 3-5 h, vacuumizing, introducing hot water to control the temperature to-20 ℃, sublimating, and removing water. Obtaining solid bacterium powder of the small strange bacteria BQB-B04 of the Kloshner, wherein the water content of the bacterium powder is less than 5% (W/W), and the viable count is 2-5 multiplied by 1011cfu/g。
(2) Preparing a bacterial liquid and solid bacterial powder of Bacillus belgii YZS-M03:
activating Bacillus beiLeisi YZS-M03 strain, picking single colony, inoculating in 150ml NB culture medium (121 deg.C, sterilizing for 30 min), 28 deg.C, 180 r.min-1Performing shake culture for 20h under the condition to obtain first-class seedsAnd (4) liquid.
B, preparing a B.beilesiensis YZS-M03 secondary seed solution:
inoculating the first-grade seed liquid into a sterile seed tank, wherein the inoculation amount is 0.5-1.0% (V/V); the culture conditions were: adjusting DO (DO 40%) by manually adjusting the rotating speed and the air flow rate through ventilation, wherein the culture temperature is 30-35 ℃, and the rotating speed is 200-350 r.min-1The tank pressure is 0.05 MPa; the culture time is 15-20 h. OD of second-stage seed liquid600The value is 0.6-0.8, and the strain activity is strongest at the moment.
Preparation of Bacillus bleekeri YZS-M03 fermentation broth:
inoculating the secondary seed liquid of Bacillus beiLeisi YZS-M03 into a sterile fermentation tank according to the inoculation amount of 0.5-1.0% (V/V), wherein the fermentation medium comprises the following components (per liter): yeast extract extraction: 10-20 g, maltose syrup: 25-35 g, magnesium chloride: 0.2-0.5 g, calcium chloride: 0.1-0.4 g, manganese chloride: 0.1-0.4 g, dipotassium hydrogen phosphate: 1.0-2.5 g. The culture conditions are as follows: adjusting DO (DO 40%) by manually adjusting the rotating speed and the air flow rate through ventilation, wherein the culture temperature is 30-35 ℃, and the rotating speed is 200-350 r.min-1The tank pressure is 0.05 MPa; the culture time is 30-35 h, the pH is raised to 7.5-8.0 and the OD is increased600The value is 1.2-1.5, and when the spores are mature for the time of putting into the tank by sampling microscopic examination, the fermentation is stopped to obtain the fermented mature bacterial liquid.
Preparation of Bacillus belgii YZS-M03 bacterial powder:
filtering the obtained mature fermentation bacterial liquid by a ceramic membrane, adding light calcium carbonate, uniformly mixing, wherein the mass ratio of the concentrated bacterial liquid to the light calcium carbonate is 100: 2-5 to obtain a solid-liquid mixture, and performing spray drying under the conditions as follows: the inlet temperature of the material is 150-200 ℃, the outlet temperature is 80-85 ℃, and the drying time of the material in the tower is 15-30S. Obtaining the Bacillus beilesiensis YZS-M03 solid bacterial powder, wherein the water content of the bacterial powder is less than 5 percent (W/W), and the viable count is 2-5 multiplied by 1011cfu/g。
The preparation of the Bacillus altitudinis ZLB-C01 bacterial liquid and the solid bacterial powder is the same as above.
The Bacillus bleekeri YZS-M03, the small stranguria Klishmi BQB-B04 and the Bacillus altitudinis ZLBUniformly mixing solid fungus powder of-C01 in a mass ratio of 1:1:1 to obtain solid mixed fungus powder, and mixing the bran and the solid mixed fungus powder in a mass ratio of 100:2 to obtain a solid preparation 1 of the growth-promoting compound microorganism fungus agent, wherein the total viable bacteria concentration is 2 x 109cfu/g。
Uniformly mixing the solid fungus powder of the Bacillus beiLeisi YZS-M03, the small stranguria Kloshimi BQB-B04 and the Bacillus altitudinis ZLB-C01 according to the mass ratio of 5:6:8 to obtain solid mixed fungus powder, and mixing the bran and the solid mixed fungus powder according to the mass ratio of 100:5 to obtain a solid preparation 2 of the growth-promoting compound microbial agent, wherein the total viable bacteria concentration is 5 multiplied by 109cfu/g。
Uniformly mixing the solid bacterial powder of the Bacillus belgii YZS-M03, the small strange bacteria BQB-B04 and the Bacillus altivelis ZLB-C01 in a mass ratio of 4:5:6 to obtain solid mixed bacterial powder, and mixing the bran and the solid mixed bacterial powder in a mass ratio of 100:3 to obtain a solid preparation 3 of the growth-promoting compound microbial agent, wherein the total viable bacteria concentration is 3 multiplied by 109cfu/g。
Mixing mature fermentation bacteria solutions of the Bacillus beiLeisi YZS-M03, the small stranguria Kloshimi BQB-B04 and the Bacillus altitudinis ZLB-C01 according to the mass ratio of 1:1:1 to obtain the growth promoting compound microbial agent liquid preparation 1, wherein the total viable bacteria concentration is 5 multiplied by 108cfu/ml。
Mixing fermented mature bacteria liquid of the Bacillus beiLeisi YZS-M03, the small stranguria Kloshimi BQB-B04 and the Bacillus altitudinis ZLB-C01 according to the mass ratio of 5:6:8 to obtain the growth promoting compound microbial agent liquid preparation 2, wherein the total viable bacteria concentration is 8 multiplied by 108cfu/ml。
Mixing fermented mature bacteria liquid of the Bacillus beiLeisi YZS-M03, the small strange bacteria BQB-B04 and the Bacillus altivelis ZLB-C01 according to the mass ratio of 4:5:6 to obtain a growth promoting compound microbial agent liquid preparation 3, wherein the total viable bacteria concentration is 6 multiplied by 108cfu/ml。
Example 2 Effect of growth-promoting Complex microbial preparation liquid preparation 1 on leaves of Brassica campestris
Growth promoting composite prepared in example 1The microbial agent liquid preparation 1 and single strain fermented mature bacterial liquid of Bacillus beiLeisi YZS-M03, Microstranguria Kleishenii BQB-B04 and Bacillus altivelis ZLB-C01 are respectively prepared into viable bacteria content of 1 × 10 with sterile water8 cfu/mL bacterial suspension, through the root irrigation inoculated rape seedlings (the inoculation amount is 20 mL/plant), each treatment 10, 3 times of repetition, with clear water as the control. And (3) measuring the content of chlorophyll after 15 days of inoculation treatment, collecting 10 pieces of plant leaves, and fully mixing the 10 pieces of plant leaves to obtain a mixed sample for measuring indexes such as Catalase (CAT), Peroxidase (POD) and superoxide dismutase (SOD).
A SPAD-502 chlorophyll rapid determination instrument (Minolta, Japan) is adopted to select 10 plant leaves, the SPAD values are respectively determined at the leaf base, the leaf and the leaf tip, the average value of each leaf is calculated, and 3 times of repetition are carried out. The relative leaf chlorophyll values are expressed as SPAD values.
The activity of Catalase (CAT) in plant leaves is measured by adopting an ultraviolet spectrophotometry, the activity of Peroxidase (POD) in the plant leaves is measured by referring to a guaiacol method, and the activity of superoxide dismutase (SOD) in the plant leaves is measured by referring to a Nitrobluetetrazolium (NBT) photoreduction method.
Influence of growth-promoting compound microbial agent on chlorophyll content of rape leaves
When the growth-promoting compound microbial agent is injected and inoculated for 15 days, the SPAD value of the rape leaves is higher than that of the clear water control treatment and the single strain mature zymocyte liquid treatment of the Bacillus beiLensis YZBS-M03, the Michelia crassipes BQB-B04 and the Bacillus altitudinii ZLB-C01, and the SPAD value of the rape leaves applied with the growth-promoting compound microbial agent is obviously different from that of the clear water control. The chlorophyll content of the rape leaves treated by the endophytic bacteria is increased, and the chlorophyll plays an important role in light energy absorption, transmission and conversion, so that the increase of the chlorophyll content can improve the photosynthesis rate of plants, thereby promoting the growth of the plants, as shown in figure 1.
Influence of growth-promoting compound microbial agent on Catalase (CAT) activity of rape leaves
As can be seen from FIG. 2, the growth-promoting complex microbial inoculant is used for treating rape leavesCatalase (CAT) activity produces obvious influence, and the catalase activity of the rape leaves after the growth promoting compound microbial agent treatment is higher than that of the rape leaves after the clear water control treatment and the single strain fermentation mature bacteria liquid treatment of the Bacillus beiLeisi YZS-M03, the Michelle Michella vulgaris BQB-B04 and the Bacillus altitudinii ZLB-C01, and the difference is obvious compared with the control treatment. After being treated by the compound microbial agent and the endophytic bacteria, the CAT activity in the rape body can be rapidly increased and maintained at a higher level. Catalase (CAT) is an important enzymatic defense system in plants, and can scavenge H2O2It is an important antioxidant enzyme in plants.
Effect of growth-promoting compound microbial agent on Peroxidase (POD) activity of rape leaves
When the growth-promoting compound microbial agent is inoculated for 15 days, the activities of the Peroxidase (POD) of the rape leaves are higher than those of the rape leaves subjected to the clear water control treatment and the single-strain mature zymophyte liquid treatment of the bacillus beiLeisi YZS-M03, the strange bacteria BQB-B04 and the bacillus altitudinis ZLB-C01, and the activities of the Peroxidase (POD) of the rape leaves subjected to the growth-promoting compound microbial agent are obviously different from those of the clear water control. It is shown that the growth-promoting complex microbial agent can increase the Peroxidase (POD) activity of plant leaves, thereby promoting the growth of plants, see FIG. 3.
Influence of growth-promoting compound microbial agent on activity of superoxide dismutase (SOD) of rape leaves
When the growth-promoting compound microbial agent is injected and inoculated for 15 days, the superoxide dismutase (SOD) activity of the rape leaves is higher than that of the clear water control treatment and the single strain mature zymocyte liquid treatment of the Bacillus beiLeisi YZS-M03, the Michelia crassipes BQB-B04 and the Bacillus altitudinis ZLB-C01, and the difference of the superoxide dismutase (SOD) activity of the rape leaves applied with the growth-promoting compound microbial agent is obvious compared with that of the clear water control. It is shown that the application of the growth-promoting compound microbial agent can improve the activity of superoxide dismutase (SOD) of plant leaves, thereby promoting the growth of plants, as shown in figure 4.
Influence of growth-promoting compound microbial agent on high growth of rape plants
After the growth-promoting microbial agent is used for treating, root irrigation and inoculation to treat rape seedlings, the high growth of the rape seedlings can be obviously promoted, the contrast treatment with clear water and the treatment with single-strain solid bacteria powder of Bacillus beiLensis YZBS-M03, Microstranguria Klishii BQB-B04 and Bacillus altitudinis ZLB-C01 are adopted, the difference is obvious (figure 5), and the effect of inoculation of the growth-promoting microbial agent on promoting the growth and development of plants is shown.
Example 3 Effect of growth-promoting Complex microbial preparation liquid preparation 2 on Shanghai green seedlings
The growth-promoting complex microbial preparation liquid preparation 2 prepared in example 1 and single strain fermentation mature bacterial liquids of Bacillus beiLeisi YZS-M03, Microstranguria Kloshenii BQB-B04 and Bacillus altitudinis ZLB-C01 were respectively prepared into a viable bacteria content of 1 × 10 with sterile water8 cfu/mL bacterial suspension, through the root irrigation inoculation of Shanghai green seedlings (the inoculation amount is 20 mL/plant), each treatment 10 plants, 3 times of repetition, with clear water as the control. Inoculating treatment for 15d to measure chlorophyll content, collecting 10 pieces of folium Gynurae Divaricatae, mixing well to obtain a mixed sample, and measuring indexes such as Catalase (CAT), Peroxidase (POD) and superoxide dismutase (SOD).
Selecting 10 Shanghai Qinghai leaves by using a SPAD-502 chlorophyll rapid determinator (Minolta, Japan), respectively measuring SPAD values at the leaf base, the leaf and the leaf tip, calculating the average value of each leaf, and repeating for 3 times. The relative leaf chlorophyll values are expressed as SPAD values.
The activity of Catalase (CAT) in plant leaves is measured by adopting an ultraviolet spectrophotometry, the activity of Peroxidase (POD) in the plant leaves is measured by referring to a guaiacol method, and the activity of superoxide dismutase (SOD) in the plant leaves is measured by referring to a Nitrobluetetrazolium (NBT) photoreduction method.
Influence of growth-promoting compound microbial agent on chlorophyll content of Shanghai green leaves
When the growth-promoting compound microbial agent is injected and inoculated for 15 days, the SPAD value of the Shanghai green leaves is higher than that of the Shanghai green leaves subjected to clear water control treatment and single strain mature fermentation liquid treatment by applying Bacillus beijerinckii YZS-M03, Schimmermatoclem closterium BQB-B04 and Bacillus altissima ZLB-C01, and the SPAD value of the Shanghai green leaves subjected to the growth-promoting compound microbial agent is remarkably different from that of the clear water control. The chlorophyll content of the Shanghai green leaves treated by the endophytic bacteria is increased, and the chlorophyll plays an important role in light energy absorption, transmission and conversion, so that the increase of the chlorophyll content can improve the photosynthesis rate of plants, thereby promoting the growth of the plants, as shown in figure 6.
Influence of growth-promoting compound microbial agent on Catalase (CAT) activity of Shanghai Qing leaves
As can be seen from FIG. 7, the Catalase (CAT) activity of the Shanghai green leaves is significantly affected by the treatment of the endophytic bacteria, and the catalase activity of the Shanghai green leaves after the treatment of the growth-promoting compound microbial agent is higher than that of the control treatment of clear water and the single strain mature fermentation liquid treatment of Bacillus beijerinckii YZS-M03, Microstranguria klishii BQB-B04 and Bacillus gibsonii ZLB-C01, which is significantly different from the control treatment. After being treated by the compound microbial agent and the endophytic bacteria, the CAT activity in the Shanghai Qing can be rapidly increased and maintained at a higher level. Catalase (CAT) is an important enzymatic defense system in plants, and can scavenge H2O2It is an important antioxidant enzyme in plants.
Influence of growth-promoting compound microbial agent on Peroxidase (POD) activity of Shanghai green leaves
When the growth-promoting compound microbial agent is inoculated for 15 days, the Peroxidase (POD) activity of the leaves of the Shanghai green is higher than that of the leaves of the Shanghai green after being treated by clear water control and treated by single strain mature fermentation liquid of Bacillus beiLeydis YZS-M03, Michelia crassifolia BQB-B04 and Bacillus altivelis ZLB-C01, and the Peroxidase (POD) activity of the leaves of the Shanghai green after being treated by the growth-promoting compound microbial agent is obviously different from that of the leaves of the clear water control. It is shown that the growth-promoting complex microbial agent can increase the Peroxidase (POD) activity of plant leaves, thereby promoting plant growth, see FIG. 8.
Influence of growth-promoting compound microbial agent on activity of Shanghai Qingqing leaf superoxide dismutase (SOD)
When the growth-promoting compound microbial agent is injected and inoculated for 15 days, the activity of the superoxide dismutase (SOD) of the Shanghai green leaves is higher than that of the clear water control treatment and the single strain mature fermentation liquid treatment of the Bacillus beiLeisi YZS-M03, the small strange bacteria BQB-B04 and the Bacillus altitudinis ZLB-C01, and the activity of the superoxide dismutase (SOD) of the Shanghai green leaves applied with the growth-promoting compound microbial agent is obviously different from that of the clear water control. It is shown that the application of the growth-promoting compound microbial agent can improve the activity of superoxide dismutase (SOD) of plant leaves, thereby promoting the growth of plants, as shown in figure 9.
Influence of growth-promoting compound microbial agent on high growth of Shanghai green plants
After the Shanghai green seedlings are treated by the growth-promoting microbial agent, root irrigation and inoculation, the growth of the plant height of the Shanghai green seedlings can be obviously promoted, the difference is obvious when the growth-promoting microbial agent is used for contrast treatment with clear water and single strain mature fermentation liquor treatment of Bacillus beijerinckii YZS-M03, Clshmiella stranguria BQB-B04 and Bacillus altissilis ZLB-C01 is applied (figure 10), and the effect of the inoculation growth-promoting microbial agent on plant growth and development is shown.
Example 4 Effect of growth promoting Complex microbial inoculant 1 on growth of strawberry
Growth-promoting complex microbial inoculant solid preparation 1 prepared in example 1 (after activation, diluted to 1 × 10 with sterile water)8cfu/mL liquid preparation for later use) and mature bacterial liquid fermented by single strains of Bacillus beiLensis YZS-M03, Michelia crassipes BQB-B04 and Bacillus altitudinis ZLB-C01, which are respectively prepared into a liquid with viable bacteria content of 1 × 10 by using sterile water8 cfu/mL of bacterial suspension. Selecting strawberry seedlings transplanted in a greenhouse for about 1 month, respectively applying a growth-promoting compound microbial agent solid preparation 1 and single strain fermentation mature bacterial liquid of Bacillus beiLeisi YZS-M03, Closimir Michthy BQB-B04 and Bacillus altitudinis ZLB-C01 to irrigate roots and inoculate the strawberry seedlings (the inoculation amount is 20 mL/strain), repeating for 3 times every 30 strains, and irrigating roots and applying the bacterial liquid again after 30 days (the inoculation amount is 20 mL/strain). The results were investigated after 60 days, using clear water treated strawberries as a control. The test result shows that compared with a clear water control group, the solid preparation 1 inoculated with the growth-promoting compound microbial agent, the Bacillus belvesii YZS-M03, the small strange bacteria BQB-B04 and the high-ground bacillus are inoculatedThe strawberries treated by the single strain fermented mature bacterial liquid of the strain ZLB-C01 have strong growth vigor and large and dark green leaves; the number of buds and blossoms is little compared with that of the control, and the number of new branches is little; the fruit setting number is obviously higher than that of the control, and the fruit setting is early and large. Compared with the clear water control treatment, the treatment of applying the compound microbial inoculant 1 has the advantages that the fruit number is increased by 20.19%, the sugar content of the strawberries is increased by 0.8, and the increase is 6.96%.
TABLE 1 Effect of Complex microbial Agents on strawberry growth
Example 5 Effect of growth promoting Complex microbial inoculum liquid microbial inoculum 3 on cucumber growth
Selecting cucumber seedlings with uniform growth vigor, four leaves and one core, 20m each in a greenhouse2The cucumber seedlings are respectively used as a test group and a control group, and the number of the cucumber seedlings in each group is the same. The growth-promoting complex microbial preparation liquid preparation 3 prepared in example 1 and single strain fermentation mature bacterial liquids of Bacillus beiLeisi YZS-M03, Microstranguria Kloshenii BQB-B04 and Bacillus altitudinis ZLB-C01 were respectively prepared into a viable bacteria content of 1 × 10 with sterile water8 cfu/mL bacterial suspension is diluted by 30 times and sprayed on leaf surfaces, and inoculated cucumber seedlings (with the inoculation amount of 20 mL/plant) are applied by root irrigation after 15 days and treated by clear water as a control. When the harvest is started, the cucumber yield, the morbidity and the like are counted for 15 consecutive days. Test results show that compared with a clear water control group, cucumbers treated by the compound microbial agent 3 and single strain fermented mature bacteria liquid of bacillus beijerinckii YZS-M03, small strange bacteria BQB-B04 and bacillus altissimus ZLB-C01 grow strongly and have dark green leaves; the cucumber shape is uniform, and the yield is obviously higher than that of the control. Compared with clear water control treatment, the compound microbial agent treatment has the yield increased by 16.09%; the incidence of the diseases is reduced.
TABLE 2 Effect of Complex microbial Agents on cucumber growth
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
SEQUENCE LISTING
<110> Minjiang academy
<120> growth-promoting compound microbial agent and application thereof
<130>
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 1398
<212> DNA
<213> Bacillus velezensis
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ggattggctt aacctcgcgg tttcgctgcc ctttgttctg tccattgtag cacgtgtgta 240
gcccaggtca taaggggcat gatgatttga cgtcatcccc accttcctcc ggtttgtcac 300
cggcagtcac cttagagtgc ccaactgaat gctggcaact aagatcaagg gttgcgctcg 360
ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc accacctgtc 420
actctgcccc cgaaggggac gtcctatctc taggattgtc agaggatgtc aagacctggt 480
aaggttcttc gcgttgcttc gaattaaacc acatgctcca ccgcttgtgc gggcccccgt 540
caattccttt gagtttcagt cttgcgaccg tactccccag gcggagtgct taatgcgtta 600
gctgcagcac taaggggcgg aaacccccta acacttagca ctcatcgttt acggcgtgga 660
ctaccagggt atctaatcct gttcgctccc cacgctttcg ctcctcagcg tcagttacag 720
accagagagt cgccttcgcc actggtgttc ctccacatct ctacgcattt caccgctaca 780
cgtggaattc cactctcctc ttctgcactc aagttcccca gttttccaat gaccctcccc 840
ggttgagccg ggggctttca catcagactt aagaaaccgc ctgcgagccc tttacgccca 900
ataattccgg acaacgcttg ccacctacgt attaccgcgg ctgctggcac gtagttagcc 960
gtggctttct ggttaggtac cgtcaaggtg ccgccctatt tgaacggcac ttgttcttcc 1020
ctaacaacag agctttacga tccgaaaacc ttcatcactc acgcggcgtt gctccgtcag 1080
actttcgtcc attgcggaag attccctact gctgcctccc gtaggagtct gggccgtgtc 1140
tcagtcccag tgtggccgat caccctctca ggtcggctac gcatcgtcgc cttggtgagc 1200
cgttacctca ccaactagct aatgcgccgc gggtccatct gtaagtggta gccgaagcca 1260
ccttttatgt ctgaaccatg cggttcagac aaccatccgg tattagcccc ggtttcccgg 1320
agttatccca gtcttacagg caggttaccc acgtgttact cacccgtccg ccgctaacat 1380
cagggagcaa gctcccat 1398
<210> 2
<211> 886
<212> DNA
<213> Advenella kashmirensis
<400> 2
ggcggaggca cggggcgagc tcgcgagaag agtgagcctg gctcagagtt tgatcctggc 60
tcgagtttga tcgaggggtg agtttgtatc ggaacgtgcc cagtagcggg ggataactac 120
gcgaaagcgt ggctaatacc gcatacgccc tacgggggaa agggggggat cttaggacct 180
ctcactattg gagcggccga tatcggatta gctagttggt ggggtaaagg cctaccaagg 240
cgacgatccg tagctggttt gagaggacga ccagccacac tgggactgag acacggccca 300
gactcctacg ggaggcagca gtggggaatt ttggacaatg ggggaaaccc tgatccagcc 360
atcccgcgtg tgcgatgaag gccttcgggt tgtaaagcac ttttgtcagg gaagaaaagg 420
tttcggataa tacctggaac tgatgacggt acctgaagaa taagcaccgg ctaactacgt 480
gccagcagcc gcggtaatac gtagggtgca agcgttaatc ggaattactg ggcgtaaagc 540
gtgcgcaggc ggttcggaaa gaaagatgtg aaatcccagg gctcaacctt ggaactgcat 600
ttttaactac cggactagag tatgtcagag gggggtggaa ttccacgtgt agcagtgaaa 660
tgcgtagata tgtggaggaa caccgatggc gaaggcagcc ccctgggata atactgacgc 720
tcatgcacga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccctaaa 780
cgatgtcaac tagctgttgg ggcccttcgg ggcttagtag cgcagctaac gcgtgaagtt 840
tgacccgccc tggggggagt acggatcgca agatttaaaa aactca 886
<210> 3
<211> 1434
<212> DNA
<213> Bacillus altitudinis
<400> 3
ctatactgca gtcgagcgga cagaagggag cttgctcccg gatgttagcg gcggacgggt 60
gagtaacacg tgggtaacct gcctgtaaga ctgggataac tccgggaaac cggagctaat 120
accggatagt tccttgaacc gcatggttca aggatgaaag acggtttcgg ctgtcactta 180
cagatggacc cgcggcgcat tagctagttg gtgaggtaac ggctcaccaa ggcgacgatg 240
cgtagccgac ctgagagggt gatcggccac actgggactg agacacggcc cagactccta 300
cgggaggcag cagtagggaa tcttccgcaa tggacgaaag tctgacggag caacgccgcg 360
tgagtgatga aggttttcgg atcgtaaagc tctgttgtta gggaagaaca agtgcaagag 420
taactgcttg caccttgacg gtacctaacc agaaagccac ggctaactac gtgccagcag 480
ccgcggtaat acgtaggtgg caagcgttgt ccggaattat tgggcgtaaa gggctcgcag 540
gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc attggaaact 600
gggaaacttg agtgcagaag aggagagtgg aattccacgg tgtagcggtg aaatgcgtag 660
agatgtggag gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggag 720
cgaaagcgtg gggagcgaac aggattagat accctggtag tccacgccgt aaacgatgag 780
tgctaagtgt tagggggttt ccgcccctta gtgctgcagc taacgcatta agcactccgc 840
ctggggagta cggtcgcaag actgaaactc aaaggaattg acgggggccc gcacaagcgg 900
tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatcctct 960
gacaacccta gagatagggc tttcccttcg gggacagagt gacaggtggt gcatggttgt 1020
cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttgatctt 1080
agttgccagc attcagttgg gcactctaag gtgactgccg gtgacaaacc ggaggaaggt 1140
ggggatgacg tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatgga 1200
cagaacaaag ggctgcgaga ccgcaaggtt tagccaatcc cacaaatctg ttctcagttc 1260
ggatcgcagt ctgcaactcg actgcgtgaa gctggaatcg ctagtaatcg cggatcagca 1320
tgccgcggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca cgagagtttg 1380
caacacccga agtcggtgag gtaaccttta tggagccagc cgccgaaggg ggac 1434
Claims (5)
1. The growth-promoting compound microbial agent is characterized in that the microbial agent comprises bacillus belgii (Bacillus belgii) ((R))Bacillus velezensis) YZS-M03, small stranguria Clashmania (C.) (Advenella kashmirensis) BQB-B04 and Bacillus altitudinis (Bacillus altitudinis) (B-04)Bacillus altitudinis) ZLB-C01; said Bacillus belgii: (Bacillus velezensis) YZS-M03, which has been deposited in China general microbiological culture Collection center at 16 months 10 and 2020, with the deposit numbers: CGMCC No. 20900; said keshmir smallStrange fungus (Advenella kashmirensis) BQB-B04 was deposited in China general microbiological culture Collection center (CGMCC) at 2021, No. 01, and the deposited numbers are: CGMCC No. 21796; the Bacillus altitudinis (A), (B), (CBacillus altitudinis) ZLB-C01 has been deposited in China general microbiological culture Collection center (CGMCC) at 10 and 16 months 2020, with the deposit numbers: CGMCC No. 20898; the concentration of total viable bacteria in the microbial inoculum is (5-8) multiplied by 108cfu/ml or (2-3). times.109cfu/g; the mass ratio of solid bacterial powder or fermentation liquor of the Bacillus beiLeisi YZS-M03, the small stranguria Klishii BQB-B04 and the Bacillus altivelis ZLB-C01 in the microbial inoculum is (3-5): (3-6): (3-8).
2. The method for preparing the growth-promoting complex microbial inoculant according to claim 1, wherein the method comprises preparing a solid preparation of the growth-promoting complex microbial inoculant or a liquid preparation of the growth-promoting complex microbial inoculant.
3. The method for preparing a solid preparation according to claim 2, comprising the steps of: mixing solid bacterial powder of Bacillus lesiensis YZS-M03, Microstranguria keliensis BQB-B04 and Bacillus altissimus ZLB-C01 to prepare solid mixed bacterial powder, and mixing a carrier and the solid mixed bacterial powder according to the mass ratio of 100: 2-5 to obtain a growth-promoting compound microbial agent solid preparation; the carrier comprises bran.
4. The method for preparing a liquid formulation according to claim 2, comprising the steps of: mixing fermentation liquor of Bacillus beiLeisi YZS-M03, small strange bacteria BQB-B04 and Bacillus altissimus ZLB-C01 in proportion.
5. The use of the growth-promoting complex microbial inoculant according to claim 1 for promoting plant growth.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635908A (en) * | 2016-12-29 | 2017-05-10 | 长安大学 | Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent |
| CN110291059A (en) * | 2017-01-12 | 2019-09-27 | 喜施倍全球股份有限公司 | Microbe soil reinforcing agent |
| CN111139206A (en) * | 2020-03-09 | 2020-05-12 | 闽江学院 | Growth-promoting compound endophytic bacteria agent and application thereof |
| CN111235064A (en) * | 2020-03-09 | 2020-06-05 | 闽江学院 | Agricultural compound microbial agent and application thereof |
| CN112342169A (en) * | 2020-11-24 | 2021-02-09 | 中国农业科学院农业资源与农业区划研究所 | Bacillus altitudinis and application thereof in prevention and control of cigar fermentation mildew |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635908A (en) * | 2016-12-29 | 2017-05-10 | 长安大学 | Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent |
| CN110291059A (en) * | 2017-01-12 | 2019-09-27 | 喜施倍全球股份有限公司 | Microbe soil reinforcing agent |
| CN111139206A (en) * | 2020-03-09 | 2020-05-12 | 闽江学院 | Growth-promoting compound endophytic bacteria agent and application thereof |
| CN111235064A (en) * | 2020-03-09 | 2020-06-05 | 闽江学院 | Agricultural compound microbial agent and application thereof |
| CN112342169A (en) * | 2020-11-24 | 2021-02-09 | 中国农业科学院农业资源与农业区划研究所 | Bacillus altitudinis and application thereof in prevention and control of cigar fermentation mildew |
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