CN112725180B - Liver cell culture device - Google Patents
Liver cell culture device Download PDFInfo
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- CN112725180B CN112725180B CN202110225128.8A CN202110225128A CN112725180B CN 112725180 B CN112725180 B CN 112725180B CN 202110225128 A CN202110225128 A CN 202110225128A CN 112725180 B CN112725180 B CN 112725180B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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Abstract
The invention discloses a liver cell culture device, wherein a box cover comprises two parts which are spliced in the middle of a square box body and can slide to two sides respectively, a driving structure at the lower part of the square box body comprises rotating shafts which are rotatably assembled on the square box body, a crank is assembled on each rotating shaft in a rotation stopping way, the crank and the box cover parts at the same side form a crank slide block mechanism through connecting rods, a supporting plate parallel to the bottom of the box body is arranged in the square box body in a lifting way, cam structures for driving the supporting plate to lift are assembled in the square box body above the two rotating shafts in a rotation stopping way respectively, and a driving structure for driving the two rotating shafts to rotate is fixed on the square box body. According to the invention, the two parts of the driving box cover are driven to slide to two sides through the rotating shaft respectively, and simultaneously the cam structure is driven to rotate, so that the supporting plate moves upwards, researchers do not need to move the culture dish, the risk of potential turning over of the culture dish due to the need of moving the culture dish when taking liver cell culture samples is reduced, and the time is saved because the culture dish does not need to be moved first.
Description
Technical Field
The invention relates to a liver cell culture device, and belongs to the field of bioengineering.
Background
The liver is an important organ of human body, its function is mainly metabolism function, in which there are mainly vitamin, hormone metabolism and water metabolism, in addition, the liver has an important function, namely detoxification function, and toxic substances produced in both exogenesis and in vivo can be discharged out of body through liver metabolism non-toxic substances, so that it is known that the liver is critical to human body, and various liver diseases have a great influence on the health of liver, many are fatal, and in order to develop liver disease therapeutic medicine better, it is necessary to culture liver cells in vitro. Liver cells need to be cultivated in a culture dish, the culture dish is generally placed in a culture box, and because the culture dish needs to be placed in the place of the culture box, when a researcher needs to take the culture dish, the culture box needs to be opened first, then the culture dish needs to be taken out and placed in other places, then a sample needs to be taken out, the culture dish needs to be moved in the process, the risk of turning over the culture dish is increased, and the moving process can waste time.
Disclosure of Invention
The invention aims to provide a liver cell culture device which solves the problems that in the prior art, when taking a liver cell culture sample, a culture dish is at risk of turning over, and time is wasted.
In order to achieve the above purpose, the present invention adopts the following technical scheme: the liver cell culture device comprises a square box body with an opening at the upper end, a box cover is assembled on the opening at the upper end of the square box body in a sliding way, the box cover comprises two parts which are spliced in the middle of the square box body and can slide to two sides respectively, two driving structures are arranged at the lower part of the square box body, each driving structure comprises a rotating shaft which is rotationally assembled on the square box body, a crank is rotationally assembled on each rotating shaft, the crank and the box cover part at the same side form a crank slide block mechanism through a connecting rod, a supporting plate parallel to the box bottom is arranged in the square box body in a lifting way, cam structures which are respectively rotationally assembled on the two rotating shafts and drive the supporting plate to rise when the two parts of the box cover are separated to two sides and drive the supporting plate to fall when the two parts of the box cover are spliced, the square box on be fixed with the pivoted drive structure of drive two pivots, two drive structure constitute the same and bilateral symmetry, be provided with synchronous rotating structure in two pivots, synchronous rotating structure including the respectively just change the assembly at two epaxial gears of structure the same and intermeshing, two parts amalgamations of case lid when two slider-crank mechanisms are in a extreme position, the length of crank is R, the length of connecting rod is R, the longest footpath of cam is L, when the layer board is in the highest position, the lower surface of layer board is h with the distance between the slider sliding surface, when two slider-crank are in another extreme position, the contained angle between crank and the horizontal plane is θ, the contained angle between the longest footpath of cam and the horizontal plane is β simultaneously, then: (r+r) Sin θ=lsin β+h, whereby θ can be derived, and the angle between the longest diameter of the crank and the cam is: and (beta-theta), wherein beta is smaller than or equal to 90 degrees, if the diameter of the culture dish is D, the height of the culture dish and the thickness of the supporting plate are H, and when the upper end of the culture dish moves to the sliding surface of the sliding block, the included angle between the longest diameter of the cam and the horizontal plane is: δ, lsin β -Lsin δ=h-H, so that δ can be obtained, and if the included angle between the straight lines where the crank is located when the crank is located at two limit positions is η, the rotated angle α when the crank is located in the buckled state with respect to the two portions of the case cover is: η - (beta-delta), so that the opening length s between two parts of the box cover when the upper end of the culture dish moves to the sliding surface of the sliding block is obtained, D is smaller than or equal to s, a lower limit block in limit fit with the lower surface of the supporting plate is arranged on the inner wall of the box body, the distance between the working surface, which is used for being in contact with the supporting plate, of the two rotating shafts of the crank is larger than or equal to Lsin delta, the Lsin delta is larger than or equal to the length r of the crank, and the two rotating shafts are driven to rotate by a rotating handle connected to one rotating shaft.
The end part of the cam structure, which is contacted with the supporting plate, is provided with a roller which is assembled in a rotating way, and the roller is matched with the supporting plate in a rolling way.
The cam structures on the two rotating shafts are in one-to-one correspondence, limiting grooves with bottoms matched with the wheel faces of the rollers are formed in the lower surface of the supporting plate, and the two cam structures in one-to-one correspondence are matched with one limiting groove.
One rotating shaft is provided with two cam structures.
Two cranks are arranged on any rotating shaft, and the cranks are all positioned on the outer side of the square box body.
The two sides of the supporting plate extend to the two side walls of the square box body respectively and are matched with the side walls of the square box body in a sliding guide manner.
One part of the box cover is provided with an upper notch which is concaved downwards from the upper surface at the position of being matched with the other part of the box cover, and the other part of the box cover is provided with a lower notch which is matched with the upper notch to enable the two parts of the box cover to be buckled.
The box cover is in sliding fit with the square box body through convex strips which are arranged at two ends outside the square box body and are in sliding fit with the outer end face of the square box body, and the corresponding connecting rods are hinged with the box cover through hinges between the connecting rods and the convex strips.
According to the invention, the two parts of the box cover are driven to slide to two sides through the rotating shaft, and simultaneously the cam structure is driven to rotate, so that the supporting plate moves upwards, the culture dish placed on the supporting plate can be lifted, a researcher can conveniently sample without moving the culture dish, the risk of potential turning over of the culture dish due to the need of moving the culture dish when taking a liver cell culture sample is reduced, and the time is saved without moving the culture dish first.
The synchronous rotating structure is arranged on the two rotating shafts and comprises gears which are assembled on the two rotating shafts in a stopping way respectively, have the same structure and are meshed with each other, the synchronous rotating structure can ensure that the two rotating shafts synchronously rotate, and the two rotating shafts can be driven by driving one rotating shaft at the same time.
The end part of the cam structure, which is contacted with the supporting plate, is provided with the roller which is assembled in a rotating way, the roller is matched with the supporting plate in a rolling way, and the existence of the roller can reduce the friction force between the cam structure and the supporting plate, so that the relative movement between the cam structure and the supporting plate is easier.
The two cam structures on the two rotating shafts are in one-to-one correspondence, the lower surface of the supporting plate is provided with the limiting grooves with bottoms matched with the wheel surfaces of the rollers, the two cam structures in one-to-one correspondence are matched with the limiting groove, and the limiting grooves can be used for realizing the positioning between the supporting plate and the cam structures and guaranteeing the stability of the supporting plate in the moving process.
The two sides of the supporting plate respectively extend to the two side walls of the square box body and are in sliding guide fit with the side walls of the square box body, so that the stability of the supporting plate in the moving process is further ensured.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is a top view of FIG. 1;
FIG. 3 is an enlarged view of a portion of FIG. 1 at A;
FIG. 4 is a schematic diagram of an embodiment of the present invention;
fig. 5 is a dimensional relationship diagram of an embodiment of the present invention.
Detailed Description
In the embodiment of the liver cell culture apparatus shown in fig. 1 to 4, the square box body 1 is provided with a box cover 2 at the upper end opening, the box cover 2 is plate-shaped and divided into two parts, the two parts of the box cover 2 are spliced at the middle part of the square box body 1, and the two parts of the box cover 2 are in sliding fit with the upper end opening part of the square box body 1, so that the two parts of the box cover 2 can slide to two sides, because the square box body 1 is square and has a length direction and a width direction, one direction is a side, the other direction is an end, and the two parts are randomly selected before the definition of the side and the end, and the definition is unchanged. When the two parts of the box cover 2 slide to two sides, the upper end opening of the square box body is opened, and the upper end opening of the square box body after the two parts of the box cover 2 are relatively slid and folded and spliced is closed. The two ends of the box cover 2 outside the square box body 1 are convexly provided with raised strips 3, the two raised strips 3 are convexly arranged on the lower surface of the box cover 2, the two raised strips 3 are in sliding fit with the outer end faces of the square box body 1, sliding strips 4 are respectively convexly arranged on the two outer end faces of the square box body 1, and the sliding strips 4 are respectively in sliding buckling with the corresponding matched grooves formed in the raised strips. Two rotating shafts 5 are rotatably assembled at the lower part of the square box body 1, the two rotating shafts 5 are arranged in parallel, and two ends of the two rotating shafts 5 extend out from two end faces of the square box body 1 respectively. Two ends of any rotating shaft 5 extending out of the square box body 1 are respectively provided with a crank 6 in a rotation stopping way, and two cranks 6 on one rotating shaft respectively form a crank slide block mechanism with the part of the box cover 2 on the side of the rotating shaft 5 through a connecting rod 7, so that the two parts of the box cover 2 can be separated or spliced by rotating the rotating shaft, and the opening of the upper end of the square box body 1 is closed and opened. A supporting plate 8 is arranged in the square box body 1, and the supporting plate 8 is parallel to the bottom plate of the square box body 1. The two sides of the supporting plate 8 extend to the two side walls of the square box body 1 respectively and are in sliding guide fit with the side walls of the square box body 1, and the two sides are matched and matched in a sliding manner through the sliding grooves and the sliding strips, which is a conventional guide matching mode and is not described in detail herein. Two cam structures 9 are respectively arranged on any rotating shaft 5 in the square box body 1 in a rotation stopping way, rollers 10 are rotatably arranged on the free ends of the cam structures 9, two rollers 10 are arranged corresponding to one cam structure 9, and the two rollers 10 are respectively arranged on two sides of the cam structure 9 and are assembled on the cam structure by one shaft. The four cam structures are in contact with the lower surface of the supporting plate 8, the four cam structures drive the supporting plate to ascend or descend along with the rotation of the two rotating shafts 5, the supporting plate 8 drives the supporting plate to descend after the two parts of the box cover are separated to two sides, and the two parts of the box cover are combined.
The crank block structure and the cam structure which are connected with the two rotating shafts 5 are bilaterally symmetrical, the two rotating shafts 5 are provided with synchronous rotating structures, the synchronous rotating structures are gears 11 which are assembled on the two rotating shafts 5 in a stopping way respectively and have the same structure and are meshed with each other, so that the rotation of the two rotating shafts can be realized by driving one rotating shaft, and the rotating handle 12 is connected with the rotating shaft 5. The rotating shaft 5 can be driven to rotate by rotating the handle, so that the two parts of the box cover are driven to open and close, and the supporting plate is lifted and lowered. The synchronous rotating structure is positioned outside the crank on the corresponding side.
The positions of the cam structures on the two rotating shafts 5 are in one-to-one correspondence, the lower surface of the supporting plate 8 is provided with limiting grooves 15 with bottoms matched with the wheel faces of the rollers, and the two cam structures in one-to-one correspondence are matched with one limiting groove 15.
At one part of the case cover 2, there is an upper notch 13 recessed from the upper surface at the point of engagement with the other part, and the other part of the case cover 2 has a lower notch 14 at which the upper notch engages to allow the two parts of the case cover to be snapped together.
The corresponding connecting rod 7 is hinged on the raised strip 3, and the two parts of the box cover can be driven to open and close by the raised strip 3.
When the two crank block mechanisms are in one limit position, two parts of the box cover are spliced, as shown in fig. 5: the length of the crank is R, the length of the connecting rod is R, the longest diameter of the cam is L, when the supporting plate is at the highest position, the distance between the lower surface of the supporting plate and the sliding surface of the sliding block is h, when the two crank sliding blocks are at the other limit position, the included angle between the crank and the horizontal plane is theta, and meanwhile, the included angle between the longest diameter of the cam and the horizontal plane is beta, then: (r+r) Sin θ=lsin β+h, whereby θ can be derived, and the angle between the longest diameter of the crank and the cam is: and (beta-theta), wherein beta is smaller than or equal to 90 degrees, if the diameter of the culture dish is D, the height of the culture dish and the thickness of the supporting plate are H, and when the upper end of the culture dish moves to the sliding surface of the sliding block, the included angle between the longest diameter of the cam and the horizontal plane is: δ, lsin β -Lsin δ=h-H, so that δ can be obtained, and if the included angle between the straight lines where the crank is located when the crank is located at two limit positions is η, the rotated angle α when the crank is located in the buckled state with respect to the two portions of the case cover is: η - (beta-delta), so that the opening length s between two parts of the box cover when the upper end of the culture dish moves to the sliding surface of the sliding block can be obtained, D is smaller than or equal to s, a lower limiting block 16 in limit fit with the lower surface of the supporting plate is arranged on the inner wall of the box body 1, and the distance between the working surface of the lower limiting block 16, which is used for being in contact with the supporting plate, and the plane where the two crank rotating shafts are located is larger than or equal to Lsin delta. Under the condition that the required culture dish size and the height of the culture dish which is required to extend out of the box body are determined, the size of the crank sliding block structure is required first, the rotation center of the crank is required to be determined in the first step, the rotation center of the crank is only required to be located in the box body, and the sliding plane of the sliding block and the upper limit position of the supporting plate can be determined after the rotation center is determined. Then determining the longest diameter L of the cam, where the longest diameter L of the cam refers to the distance from the cam rotation center to the furthest point on the cam working surface from the cam rotation center, where L should be greater than the sum of the height of the culture dish and the thickness H of the support plate and the size of the culture dish that needs to extend out of the box, where β is equal to 90 degrees, after determining L, β is obtained, since the opening distance of the two parts of the box cover needs to be greater than the diameter of the culture dish before the upper end of the culture dish reaches the box cover, the culture dish cannot touch the box cover, and movement cannot be performed because of this, where the formula Lsin β -Lsin δ=h, so that δ is the included angle between the longest diameter of the cam and the horizontal plane when the upper end of the culture dish moves to the sliding surface of the slider, where S is equal to or greater than 90 degrees, in order to ensure that the opening diameter of the culture dish is not equal to or greater than 90 degrees, and in order to ensure that the opening of the box cover is not sufficient, the cam cannot touch the box cover, since the culture dish cannot touch the box cover, and movement cannot be performed, and since the formula Lsin β -Lsin δ=h, and δ=h, where the working distance between the upper end of the culture dish and the upper end of the culture dish is not equal to the opening to the slide is equal to or greater than the required to the required length of the plane. And in an embodiment the stroke of the slide is chosen to be equal to the diameter of the culture dish, whereby the dimensions of the crank slide structure can be determined, the determination of which is a routine choice for a person skilled in the art. From the formula (r+r) Sin θ=lsin β+h, θ can be derived, thereby accurately deriving the angle between the longest diameters of the crank and the cam: the relation between the crank and the cam can be derived from (beta-theta), and the whole power structure is determined. And the size of the culture dish and the height of the culture dish extending out of the box body are accurately ensured.
The driving structure of this embodiment is bilaterally symmetrical and is provided with a synchronous rotation structure, so that the purpose of driving two rotating shafts can be achieved by driving one rotating shaft to rotate, and in other embodiments, a separate driving mode can be adopted.
The rotating shaft of the embodiment is driven to rotate by the rotating handle, and the manual rotation can adjust the rotating speed according to actual conditions.
Claims (8)
1. A liver cell culture device, includes upper end open-ended square box, its characterized in that: the upper end opening of square box on sliding fit have the case lid, the case lid includes in square box middle amalgamation and can drive the supporting plate and rise to both sides gliding two parts respectively, square box's lower part be provided with two actuating structure, arbitrary actuating structure all including rotating the pivot of assembly on square box, the pivot on end turn and be equipped with the crank, crank and homonymy's case lid part pass through the connecting rod and constitute slider crank mechanism, square box in go up and down to be provided with the layer board that is on a parallel with the bottom of the case, end turn respectively in square box in two pivots and be equipped with the cam structure that drives the supporting plate and rise when two parts of case lid are to both sides separation, the two parts of case lid merge and drive the supporting plate and descend, square box on be fixed with drive two pivot pivoted actuating structure, two actuating structure constitute the same and bilateral symmetry, be provided with synchronous rotating structure on the two pivots, synchronous rotating structure including end turn respectively and be equipped with the same and intermeshing's of structure on two pivots, slider crank mechanism is in the diameter of a limit position, the slider is the diameter of two end of a crank, the slider is the diameter of a slider is the diameter of two, the diameter of the slider is the same, the diameter of the slider is the diameter of the two end of the slider, the diameter of the slider is the diameter of the slider, and the diameter of the slider is the length of the two, and the diameter of the slider is the length of the diameter of the slider, and the length of the slider is the diameter of the length of the slider, and the length is the diameter of the length of the slider is: (r+r) Sin θ=lsin β+h, whereby θ can be derived, and the angle between the longest diameter of the crank and the cam is: and (beta-theta), wherein beta is smaller than or equal to 90 degrees, if the diameter of the culture dish is D, the height of the culture dish and the thickness of the supporting plate are H, and when the upper end of the culture dish moves to the sliding surface of the sliding block, the included angle between the longest diameter of the cam and the horizontal plane is: δ, lsin β -Lsin δ=h-H, so that δ can be obtained, and if the included angle between the straight lines where the crank is located when the crank is located at two limit positions is η, the rotated angle α when the crank is located in the buckled state with respect to the two portions of the case cover is: η - (beta-delta), so that the opening length s between two parts of the box cover when the upper end of the culture dish moves to the sliding surface of the sliding block is obtained, D is smaller than or equal to s, a lower limit block in limit fit with the lower surface of the supporting plate is arranged on the inner wall of the box body, the distance between the working surface, which is used for being in contact with the supporting plate, of the two rotating shafts of the crank is larger than or equal to Lsin delta, the Lsin delta is larger than or equal to the length r of the crank, and the two rotating shafts are driven to rotate by a rotating handle connected to one rotating shaft.
2. The liver cell culture device of claim 1, wherein: the end part of the cam structure, which is contacted with the supporting plate, is provided with a roller which is assembled in a rotating way, and the roller is matched with the supporting plate in a rolling way.
3. The liver cell culture device of claim 2, wherein: the cam structures on the two rotating shafts are in one-to-one correspondence, limiting grooves with bottoms matched with the wheel faces of the rollers are formed in the lower surface of the supporting plate, and the two cam structures in one-to-one correspondence are matched with one limiting groove.
4. A liver cell culture device according to claim 3, wherein: one rotating shaft is provided with two cam structures.
5. The liver cell culture device of claim 4 wherein: two cranks are arranged on any rotating shaft, and the cranks are all positioned on the outer side of the square box body.
6. The liver cell culture device of claim 5 wherein: the two sides of the supporting plate extend to the two side walls of the square box body respectively and are matched with the side walls of the square box body in a sliding guide manner.
7. The liver cell culture device of claim 6 wherein: one part of the box cover is provided with an upper notch which is concaved downwards from the upper surface at the position of being matched with the other part of the box cover, and the other part of the box cover is provided with a lower notch which is matched with the upper notch to enable the two parts of the box cover to be buckled.
8. The liver cell culture device of claim 7 wherein: the box cover is in sliding fit with the square box body through convex strips which are arranged at two ends outside the square box body and are in sliding fit with the outer end face of the square box body, and the corresponding connecting rods are hinged with the box cover through hinges between the connecting rods and the convex strips.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110225128.8A CN112725180B (en) | 2021-03-01 | 2021-03-01 | Liver cell culture device |
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|---|---|---|---|
| CN202110225128.8A CN112725180B (en) | 2021-03-01 | 2021-03-01 | Liver cell culture device |
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| CN112725180B true CN112725180B (en) | 2023-06-06 |
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