CN112725144A - Preservation device for fermentation cotton stalk hydrolysis sugar liquid oil production walnut endophytic bacteria HB1310 - Google Patents
Preservation device for fermentation cotton stalk hydrolysis sugar liquid oil production walnut endophytic bacteria HB1310 Download PDFInfo
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- CN112725144A CN112725144A CN202110086917.8A CN202110086917A CN112725144A CN 112725144 A CN112725144 A CN 112725144A CN 202110086917 A CN202110086917 A CN 202110086917A CN 112725144 A CN112725144 A CN 112725144A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention discloses a preservation device for endophytic bacteria HB1310 of walnut oil production by fermented cotton stalk hydrolyzed sugar liquor, which comprises a shell mechanism, a combination mechanism, a freezing mechanism, a connecting mechanism, a sealing mechanism and a storage mechanism; the combined mechanism is arranged on the shell mechanism, the freezing mechanism is arranged in the shell mechanism through the connecting mechanism, the sealing mechanism is fixedly arranged on the upper surface in the shell mechanism and is positioned right above the freezing mechanism, and the storage mechanism is arranged in the freezing mechanism; according to the invention, by arranging the combination mechanism, a plurality of devices can be combined, so that different strains can be conveniently classified and placed, and a large amount of space can be saved; through the arrangement of the box door and the connecting mechanism, the strain can be more conveniently put in and taken out by workers; through setting up sealing mechanism, can play sealed effect to freezing mechanism, and can guarantee to the clean of freezer inside to some extent.
Description
Technical Field
The invention relates to the technical field of microorganism preservation devices. In particular to a storage device for walnut endophytic bacteria HB1310 produced by fermenting cotton stalk hydrolysis sugar liquor.
Background
In genetic engineering, the constructed strain needs to be stored at low temperature or even ultralow temperature, and the cultured strain is placed in a test tube and then stored in a low-temperature environment so as to be in a dormant state; and the low temperature environment that is used for saving the bacterial in the current application is the thing of refrigerator class mostly, and is bulky, when preserving the test tube that is equipped with the bacterial with this kind of method, can not fix the test tube to when taking out or adorning the bacterial liquid, need handheld test tube or need solitary test-tube rack, it is very inconvenient.
Disclosure of Invention
Therefore, the invention aims to provide a preservation device for the bacteria HB1310 produced in the walnut by the fermentation cotton stalk hydrolysis sugar liquid, which can keep low temperature and can fix the preservation device.
In order to solve the technical problems, the invention provides the following technical scheme: the preservation device for the endophytic bacteria HB of the walnut for producing the oil by hydrolyzing the sugar liquor through the fermented cotton stalks comprises a shell mechanism, a combination mechanism, a freezing mechanism, a connecting mechanism, a sealing mechanism and a storage mechanism;
the combined mechanism is arranged on the shell mechanism, the freezing mechanism is arranged in the shell mechanism through the connecting mechanism, the sealing mechanism is fixedly arranged on the upper surface of the inner part of the shell mechanism and is positioned right above the freezing mechanism, and the storage mechanism is arranged in the freezing mechanism.
The preservation device for the endophytic bacteria HB in the walnut for producing the oil by the fermented cotton stalk hydrolyzed sugar solution comprises a shell mechanism, a shell mechanism and a cover mechanism, wherein the shell mechanism comprises a box body, a first radiating fin, a box door, a hinge, a first handle, a controller, abrasive paper and a first spring;
the box body is a square box body, first cooling fins penetrate through the inner parts of two side walls of the box body, and the number of the first cooling fins is four; the front side of the box body is connected with two box doors through hinge hinges;
the front surfaces of the two box doors are symmetrically provided with two first handles; the front surface of one box door is fixedly provided with a controller, and the front surface of the other box door is fixedly provided with abrasive paper;
the back of the interior of the box body is fixedly provided with first springs, and the number of the first springs is determined by actual needs.
The preservation device for the endophytic bacteria HB of the walnut for producing the oil by hydrolyzing the sugar liquor through the fermented cotton stalk comprises a sliding chute, a first sliding block, a mounting cavity, a second sliding block, a baffle plate, a shifting block, a mounting hole, a baffle block, a clamping block, a baffle ring, a second spring and a clamping hole;
the sliding grooves are inverted T-shaped, the sliding grooves are formed in the upper surface of the top wall of the box body, and the number of the sliding grooves is two; the first sliding blocks are fixedly arranged on the lower surface of the box body, the first sliding blocks are in an inverted T shape, the length of each first sliding block is larger than the width of the box body, the number of the first sliding blocks is two, the two first sliding blocks are matched with the sliding grooves, and the two first sliding blocks and the two sliding grooves are respectively positioned on the same axis;
mounting cavities are formed in two ends of the first sliding block, the height of the center of each mounting cavity is larger than that of the two sides of each mounting cavity, second sliding blocks are arranged inside the mounting cavities, the number of the second sliding blocks in each mounting cavity is two, blocking pieces are fixedly mounted on one sides of the two second sliding blocks corresponding to each other, and the blocking pieces are located inside narrow positions of the two sides of each mounting cavity; the front surfaces of the two second sliding blocks are fixedly provided with shifting blocks, the size of each shifting block is smaller than that of the second sliding block, the shifting blocks protrude out of the first sliding block, and the first sliding block is provided with a sliding hole only for the shifting blocks to slide;
mounting holes are formed in the positions, close to the top end, of the two sides of the box body, and a stop block is fixedly mounted at the outlet of each mounting hole; a clamping block is arranged inside the mounting hole, the clamping block is in an irregular inverted door shape, a retaining ring is fixedly mounted at one end of the clamping block, which is positioned inside the mounting hole, and the diameter of the retaining ring is larger than that formed between the clamping blocks; a second spring is fixedly arranged at the inner end of the mounting hole, and the other end of the second spring is fixedly connected with the clamping block;
the both sides of box have all been seted up the card hole, the card hole is located the box both sides respectively and is close to bottom department and the below of mounting hole.
The preservation device for the endophytic bacteria HB of the walnut for producing the oil by hydrolyzing the sugar liquor through the fermented cotton stalks comprises a freezing box, a heat insulation plate, a semiconductor refrigeration sheet, a heat insulation layer, a placing hole, a third spring, a heat insulation interlayer, a second handle and a temperature sensor;
the refrigerator is characterized in that the freezing box is arranged in the box body, the freezing box is a square box body with an opening at the top, and heat insulation plates are fixedly arranged on the inner surfaces of the freezing box; semiconductor refrigerating sheets are fixedly arranged on both sides of the heat insulation plate;
the heat preservation layers are fixedly mounted on the other sides of the semiconductor refrigeration sheets, the number of the heat preservation layers is two, a plurality of placing holes are formed in the two heat preservation layers, the top ends of the placing holes penetrate through the heat preservation layers, and a plurality of third springs are fixedly mounted on the lower surfaces of the interior of the placing holes; a heat-insulating interlayer is fixedly arranged between the two heat-insulating layers;
a second handle is fixedly arranged on the front surface of the freezing box; a temperature sensor is fixedly arranged at the joint of the inside of the heat preservation layer and the placing hole and connected with a controller.
The storage device for the endophytic bacteria HB of the walnut for producing the oil by hydrolyzing the sugar liquor through the fermented cotton stalk comprises a slide rail, a third slide block, a first limiting block, a second limiting block, a movable stop block, a pull rod, a fourth spring, a stop ring and a protection box;
sliding rails are fixedly mounted on two sides of the interior of the box body, the number of the sliding rails on each side of the box body is two, the sliding rails on the two sides are symmetrically arranged, third sliding blocks are fixedly mounted on two sides of the freezing box, and the third sliding blocks are slidably mounted in sliding grooves in the sliding rails;
the first limiting blocks are fixedly arranged at the positions, close to the back, of the two sides of the freezing box, and the first limiting blocks are also fixedly arranged at the positions, close to the front, of the right side of the freezing box; a second limiting block is fixedly arranged at the position, close to the front outlet, of the left side wall in the box body;
a pull rod penetrates through the right side wall of the box body and is symmetrical to the second limiting block, a movable stop block is fixedly mounted at one end of the pull rod, which is positioned in the box body, and the front surface of the movable stop block is a curved surface; a fourth spring is arranged on the part of the outer surface of the pull rod, which is positioned in the box body, and the fourth spring is positioned between the movable stop block and the box body; a resistance ring is fixedly arranged on the outer surface of the pull rod positioned outside the box body; a protective box is fixedly arranged on the outer surface of the box body, and the part of the pull rod, which is positioned outside the box body, is positioned inside the protective box;
under the natural state, the end of the movable stop block far away from the box body is located at the left position in the middle of the first limiting block.
The storage device for the endophytic bacteria HB of the walnut for producing the oil by the hydrolyzed sugar liquor of the fermented cotton stalk comprises a sealing mechanism, a sealing mechanism and a sealing ring, wherein the sealing mechanism comprises a pneumatic telescopic rod, a sealing cover and the sealing ring;
the pneumatic telescopic rod is fixedly arranged on the upper surface of the interior of the box body, the position of the pneumatic telescopic rod is determined by the freezing box, and the pneumatic telescopic rod is controlled by the controller; the bottom end of the pneumatic telescopic rod is fixedly provided with a sealing cover, the bottom end of the pneumatic telescopic rod is positioned in the center of the sealing cover, and the size of the bottom surface of the sealing cover is equal to that of the top surface of the freezer; the sealing ring is fixedly installed on the outer surface of the sealing cover, the bottom end of the sealing ring protrudes out of the sealing cover, and after the sealing cover covers the freezing box, the sealing ring is tightly attached to the outer surface of the freezing box.
The storage device for the endophytic bacteria HB of the walnut for producing the oil by the fermented cotton stalk hydrolyzed sugar solution comprises a pipe body, a pipe support, a pipe cover, a sealing gasket, an inoculating ring and a lifting ring;
the tube body is a transparent glass tube, the bottom of the tube body is conical, a tube support is arranged at the bottom of the tube body in a threaded mode, and the tube support is matched with the conical bottom of the tube body; the pipe body is placed on the fourth spring through the pipe bracket;
the top end of the pipe body is in threaded connection with a pipe cover, and a sealing gasket is arranged at the joint of the pipe cover and the pipe body; the bottom end of the pipe cover is fixedly provided with an inoculating ring, and the inoculating ring penetrates through the sealing gasket and extends to the bottom of the pipe body; the upper surface of the pipe cover is fixedly provided with a lifting ring.
The technical scheme of the invention achieves the following beneficial technical effects:
1. according to the invention, by arranging the combination mechanism, a plurality of devices can be combined, so that different strains can be conveniently classified and placed, and a large amount of space can be saved; when a plurality of devices are required to be combined, the first sliding block is scratched from one end of the sliding groove of the other device, and after the sliding is completely scratched, the shifting blocks at the two ends of the first sliding block fluctuate because the length of the first sliding block is greater than that of the sliding groove, so that the second sliding block is pushed to slide towards the two sides in the installation cavity until the blocking pieces slide out of the two sides of the installation cavity, the blocking pieces at the two ends slide out, and the blocking pieces at the two ends just fix the first sliding block on the upper surface of the other device; the fixture block on pulling both sides, rotatory one hundred eighty degrees back pine behind the card hole is pulled out to the one end that the fixture block is short, because the effect of second spring, the one end that the fixture block is short can insert in the card hole of another this device bottom, has further consolidated the connection stability of two this devices, and wherein the dog can prevent the long one end roll-off mounting hole of fixture block with keeping off the ring.
2. According to the invention, through arranging the box door and the connecting mechanism, the strain can be more conveniently put in and taken out by workers; when the strains are required to be put into or taken out of the freezer, the pull rod is pulled outwards slightly while the second handle is pulled after the box door is opened, so that the movable stop block exceeds the position of the first limit block, the freezing box can run in the slide rail through the third slide block, and then the freezing box is pulled to the outside of the box body, wherein the second limit block and the rebound movable stop block can prevent the freezer from sliding out of the freezer body, when the freezer slides out of the freezer body, the placing hole in the freezing box can be completely exposed to the outside, so that the worker can directly place the tube body filled with the strains into the placing hole, when the pipe body is placed in the placing hole, the freezing box is pushed into the box body, when the freezing box is about to completely enter the box body, the first limiting block on the right front side can extrude the curved surface of the movable stop block, after the freezer enters the box body, the movable stop block rebounds under the action of the spring to block the first limiting block.
3. According to the invention, the sealing mechanism is arranged, so that the sealing effect on the refrigerating mechanism can be achieved, and the cleanness of the interior of the refrigerating box can be guaranteed; after pushing the freezer into the box, back mounted's first spring can extrude the freezer in the box, and is motionless until the extrusion, at this moment the freezer just in time is located sealed lid under, promotes sealed lid through controller control pneumatic telescopic handle and descends, just in time with the perfect top of covering the freezer of sealed lid, the sealing ring hugs closely around the freezer, plays sealed effect.
4. According to the invention, the first spring is arranged, so that the damage to strains in the refrigerator caused by the fact that the refrigerator collides with the rear wall of the refrigerator body at an excessively high speed when the refrigerator enters the refrigerator body can be prevented.
5. According to the invention, through the arrangement of the third spring, the pipe body can be prevented from being damaged when the speed for placing the pipe body into the placing hole by a worker carelessly is too high.
6. According to the invention, through arranging the temperature sensor, a worker can know the temperature inside the placing hole through the display on the controller.
7. According to the invention, through arranging the first slide block, not only can a plurality of devices be stacked, but also the second slide block positioned at the lowest layer can be used as a base.
Drawings
FIG. 1 is a schematic cross-sectional view of the present invention;
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is a schematic bottom view of the first slider of the present invention;
FIG. 4 is an enlarged view of the structure at A in FIG. 1 according to the present invention;
FIG. 5 is an enlarged view of FIG. 1 at B according to the present invention;
FIG. 6 is a schematic top view of the freezer of the present invention;
FIG. 7 is a schematic view of a tube structure according to the present invention.
The reference numbers in the figures denote: 100-a housing mechanism; 200-a combination mechanism; 300-a freezing mechanism; 400-a connection mechanism; 500-a sealing mechanism; 600-a storage mechanism; 101-a box body; 102-a first heat sink; 103-a box door; 104-a hinge; 105-a first handle; 106-a controller; 107-abrasive paper; 108-a first spring; 201-a chute; 202-a first slider; 203-a mounting cavity; 204-a second slider; 205-a baffle plate; 206-a shifting block; 207-mounting holes; 208-a stop; 209-fixture block; 210-a baffle ring; 211-a second spring; 212-card hole; 301-freezer; 302-a heat insulation board; 303-semiconductor refrigerating sheet; 304-an insulating layer; 305-placing holes; 306-a third spring; 307-heat preservation interlayer; 308-a second handle; 309-temperature sensor; 401-a slide rail; 402-a third slider; 403-a first stopper; 404-a second stopper; 405-moving the stopper; 406-a pull rod; 407-a fourth spring; 408-a resistance ring; 409-protective box; 501-a pneumatic telescopic rod; 502-a sealing cover; 503-sealing ring; 601-a tube body; 602-a pipe bracket; 603-a tube cover; 604-a seal gasket; 605-an inoculating loop; 606-bails.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The preservation device for the endophytic bacteria HB1310 of the walnut for producing the oil by hydrolyzing the sugar liquor through the fermented cotton stalks is characterized by comprising a shell mechanism 100, a combination mechanism 200, a freezing mechanism 300, a connecting mechanism 400, a sealing mechanism 500 and a storage mechanism 600; the combination mechanism 200 is disposed on the housing mechanism 100, the freezing mechanism 300 is disposed inside the housing mechanism 100 through the connecting mechanism 400, the sealing mechanism 500 is fixedly mounted on the upper surface inside the housing mechanism 100, the sealing mechanism 500 is located right above the freezing mechanism 300, and the storage mechanism 600 is disposed inside the freezing mechanism 300.
As shown, the housing mechanism 100 includes a case 101, a first heat sink 102, a door 103, a hinge 104, a first handle 105, a controller 106, abrasive paper 107, a first spring 108; the box body 101 is a square box body, the inner parts of two side walls of the box body 101 are respectively provided with four first radiating fins 102 in a penetrating way; the front surface of the box body 101 is hinged with two box doors 103 through hinges 104, and the number of the box doors 103 is two; the front surfaces of the two box doors 103 are symmetrically provided with two first handles 105, and the number of the first handles 105 is two; the front surface of one box door 103 is fixedly provided with a controller 106, and the front surface of the other box door 103 is fixedly provided with abrasive paper 107; the back surface inside the box body 101 is fixedly provided with first springs 108, and the number of the first springs 108 is determined by actual needs; the combined mechanism 200 comprises a sliding groove 201, a first sliding block 202, a mounting cavity 203, a second sliding block 204, a blocking piece 205, a shifting block 206, a mounting hole 207, a blocking block 208, a blocking block 209, a blocking ring 210, a second spring 211 and a blocking hole 212; the sliding grooves 201 are inverted T-shaped, the sliding grooves 201 are formed in the upper surface of the top wall of the box body 101, and the number of the sliding grooves 201 is two; the first sliding blocks 202 are fixedly arranged on the lower surface of the box body 101, the first sliding blocks 202 are in an inverted T shape, the length of each first sliding block 202 is larger than the width of the box body 101, the number of the first sliding blocks 202 is two, the two first sliding blocks 202 are matched with the sliding grooves 201, and the two first sliding blocks 202 and the two sliding grooves 201 are respectively positioned on the same axis; mounting cavities 203 are formed in two ends of the first sliding block 202, the height of the center of each mounting cavity 203 is larger than that of the two sides of each mounting cavity 203, second sliding blocks 204 are arranged inside the mounting cavities 203, the number of the second sliding blocks 204 inside each mounting cavity 203 is two, blocking pieces 205 are fixedly arranged on one corresponding side of each second sliding block 204, and the blocking pieces are located inside narrow positions on two sides of each mounting cavity 203; the front surfaces of the two second sliding blocks 204 are fixedly provided with shifting blocks 206, the size of each shifting block 206 is smaller than that of the second sliding block 204, the shifting blocks 206 protrude out of the first sliding block 202, and the first sliding block 202 is provided with a sliding hole only for the shifting block 206 to slide; mounting holes 207 are formed in the positions, close to the top end, of the two sides of the box body 101, and a stop block 208 is fixedly mounted at the outlet of each mounting hole 207; a fixture block 209 is arranged in the mounting hole 207, the fixture block 209 is in an irregular inverted door shape, a stop ring 210 is fixedly mounted at one end of the fixture block 209 positioned in the mounting hole 207, and the diameter of the stop ring 210 is larger than that formed between the stop blocks 208; a second spring 211 is fixedly installed at the inner end of the installation hole 207, and the other end of the second spring 211 is fixedly connected with the latch 209; clamping holes 212 are formed in the two sides of the box body 101, and the clamping holes 212 are respectively located at the two sides of the box body 101 close to the bottom end and below the mounting holes; the freezing mechanism 300 comprises a freezing box 301, a heat insulation plate 302, a semiconductor refrigeration sheet 303, a heat insulation layer 304, a placing hole 305, a third spring 306, a heat insulation interlayer 307, a second handle 308 and a temperature sensor 309; the freezing box 301 is arranged in the box body 101, the freezing box 301 is a square box body with an opening at the top, and heat insulation plates 302 are fixedly arranged on the inner surfaces of the freezing box 301; the semiconductor refrigerating pieces 303 are fixedly arranged on the two sides of the heat insulation plate 302; the insulating layers 304 are fixedly mounted on the other sides of the semiconductor refrigeration sheets 303, the number of the insulating layers 304 is two, a plurality of placing holes 305 are formed in the two insulating layers 304, the top ends of the placing holes 305 penetrate through the insulating layers, and a plurality of third springs 306 are fixedly mounted on the lower surfaces of the inner portions of the placing holes 305; a heat-insulating interlayer 307 is fixedly arranged between the two heat-insulating layers 304; a second handle 308 is fixedly arranged on the front surface of the freezing box 301; a temperature sensor 309 is fixedly arranged at the connection part of the interior of the insulating layer 304 and the placing hole 305, and the temperature sensor 309 is connected with the controller 106; the connecting mechanism 400 comprises a sliding rail 401, a third sliding block 402, a first limiting block 403, a second limiting block 404, a movable stop 405, a pull rod 406, a fourth spring 407, a stop ring 408 and a protection box 409; sliding rails 401 are fixedly mounted on two sides of the interior of the box body 101, the number of the sliding rails 401 on each side of the box body 101 is two, the sliding rails 401 on the two sides are symmetrically arranged, third sliding blocks 402 are fixedly mounted on two sides of the freezing box 301, and the third sliding blocks 402 are slidably mounted in sliding grooves in the sliding rails 401; the first limiting blocks 403 are fixedly mounted at the positions, close to the back, of the two sides of the freezing box 301, and the first limiting blocks 403 are also fixedly mounted at the positions, close to the front, of the right side of the freezing box 301; a second limiting block 404 is fixedly arranged on the left side wall of the interior of the box body 101 close to the front outlet; a pull rod 406 penetrates through the right side wall of the box body 101 and is in symmetrical position with the second limiting block 404, a movable stopper 405 is fixedly installed at one end of the pull rod 406, which is positioned in the box body 101, and the front surface of the movable stopper 405 is a curved surface; a fourth spring 407 is arranged on the part of the outer surface of the pull rod 406, which is positioned in the box body 101, and the fourth spring 407 is positioned between the movable stopper 405 and the box body 101; a resistance ring 408 is fixedly arranged on the outer surface of the pull rod 406 positioned outside the box body 101; a protection box 409 is fixedly arranged on the outer surface of the box body 101, and the part of the pull rod 406, which is positioned outside the box body 101, is positioned inside the protection box 409; in a natural state, one end of the movable stopper 405 far away from the box body 101 is positioned at the left position in the middle of the first limiting block 403; the sealing mechanism 500 comprises a pneumatic telescopic rod 501, a sealing cover 502 and a sealing ring 503; the pneumatic telescopic rod 501 is fixedly arranged on the upper surface of the interior of the box body 101, the position of the pneumatic telescopic rod 501 is determined by the freezing box 301, and the pneumatic telescopic rod 501 is controlled by the controller 106; the bottom end of the pneumatic telescopic rod 501 is fixedly provided with a sealing cover 502, the bottom end of the pneumatic telescopic rod 501 is positioned at the center of the sealing cover 502, and the size of the bottom surface of the sealing cover 502 is equal to that of the top surface of the freezing box 301; the sealing ring 503 is fixedly arranged on the outer surface of the sealing cover 502, the bottom end of the sealing ring 503 protrudes out of the sealing cover 502, and after the sealing cover 502 is covered on the freezer 301, the sealing ring 503 is tightly attached to the outer surface of the freezer 301; the storage mechanism 600 comprises a pipe body 601, a pipe bracket 602, a pipe cover 603, a sealing gasket 604, an inoculating loop 605 and a lifting loop 606; the tube body 601 is a transparent glass tube, the bottom of the tube body is conical, a tube support 602 is installed at the bottom of the tube body 601 through threads, and the tube support 602 is matched with the conical bottom of the tube body 601; the tube body 601 is placed on the fourth spring 407 through a tube bracket; a pipe cover 603 is connected to the top end of the pipe body 601 in a threaded manner, and a sealing gasket 604 is arranged at the joint of the pipe cover 603 and the pipe body 601; an inoculating loop 605 is fixedly installed at the bottom end of the tube cover 603, and the inoculating loop 605 penetrates through the sealing gasket 604 and extends to the bottom of the tube body 601; a lifting ring 606 is fixedly mounted on the upper surface of the pipe cover 603.
The working principle is as follows: when a plurality of devices need to be combined, the first sliding block 202 is drawn in from one end of the sliding groove 201 of another device, and after the devices are completely drawn in, because the length of the first sliding block 202 is greater than that of the sliding groove 201, the shifting blocks 206 at two ends of the first sliding block 202 are shifted, so that the second sliding block 202 is pushed to slide towards two sides in the installation cavity 203 until the blocking pieces 205 slide out of two sides of the installation cavity 203, the blocking pieces 205 at two ends slide out, and the blocking pieces 205 at two ends just fix the first sliding block 202 on the upper surface of another device; the clamping blocks 209 on the two sides are pulled, the short end of the clamping block 209 is pulled out of the clamping hole 212 and then is rotated by one hundred eighty degrees and then is loosened, due to the action of the second spring 211, the short end of the clamping block 209 can be inserted into the clamping hole 212 at the bottom end of the other device, the connection stability of the two devices is further enhanced, and the clamping block 208 and the retaining ring 210 can prevent the long end of the clamping block 209 from sliding out of the mounting hole 207; when strains need to be put into or taken out of the freezer compartment 301, the second handle 308 is pulled and the pull rod 406 is pulled outwards slightly after the compartment door 103 is opened, so that the movable stopper 405 exceeds the position of the first stopper 403, the freezer compartment 301 can move in the slide rail 401 through the third slider 402, and the freezer compartment 301 is pulled out of the compartment 101, wherein the second stopper 404 and the rebounded movable stopper 405 can prevent the freezer compartment 301 from sliding out of the compartment 101, when the freezer compartment 301 slides down outside the compartment 101, the placing hole 305 in the freezer compartment 301 can be completely exposed, so that an operator can directly put the tube body 601 containing the strains into the placing hole 305, after putting the tube body 601 into the placing hole 305, push the freezer compartment 301 into the compartment 101, when the freezer compartment 301 is about to completely enter the compartment 101, the first stopper 403 on the right front side can extrude the curved surface of the movable stopper 405, after the freezer 301 enters the box body 101, the movable stopper 405 rebounds under the action of the fourth spring 407 to block the first stopper 403; after the freezing box 301 is pushed into the box body 101, the first spring 108 arranged on the back surface in the box body 101 can extrude the freezing box 301 until the freezing box 301 is extruded immovably, at the moment, the freezing box 301 is just positioned under the sealing cover 502, the controller 106 controls the pneumatic telescopic rod 501 to push the sealing cover 502 to descend, the sealing cover 502 is just covered on the top of the freezing box 301 perfectly, and the sealing ring 503 is tightly attached to the periphery of the freezing box 301 to play a role in sealing.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications are possible which remain within the scope of the appended claims.
Claims (7)
1. The preservation device for the endophytic bacteria HB1310 of the walnut produced by hydrolyzing the sugar liquor by the fermented cotton stalk is characterized by comprising a shell mechanism (100), a combination mechanism (200), a freezing mechanism (300), a connecting mechanism (400), a sealing mechanism (500) and a storage mechanism (600);
the combined mechanism (200) is arranged on the shell mechanism (100), the freezing mechanism (300) is arranged inside the shell mechanism (100) through the connecting mechanism (400), the sealing mechanism (500) is fixedly arranged on the upper surface inside the shell mechanism (100), the sealing mechanism (500) is positioned right above the freezing mechanism (300), and the storage mechanism (600) is arranged inside the freezing mechanism (300).
2. The preservation device for the endophytic bacteria HB1310 of the walnut produced by the hydrolyzed sugar solution of the fermented cotton stalk according to claim 1, characterized in that the shell mechanism (100) comprises a box body (101), a first cooling fin (102), a box door (103), a hinge (104), a first handle (105), a controller (106), abrasive paper (107) and a first spring (108);
the box body (101) is a square box body, first cooling fins (102) penetrate through the inner portions of two side walls of the box body (101), and the number of the first cooling fins (102) is four; the front surface of the box body (101) is hinged with two box doors (103) through hinges (104), and the number of the box doors (103) is two;
the front surfaces of the two box doors (103) are symmetrically provided with two first handles (105), and the number of the first handles (105) is two; the front surface of one box door (103) is fixedly provided with a controller (106), and the front surface of the other box door (103) is fixedly provided with abrasive paper (107);
the back surface of the interior of the box body (101) is fixedly provided with first springs (108), and the number of the first springs (108) is determined by actual needs.
3. The preservation device for the endophytic bacteria HB1310 of the walnut produced by the hydrolyzed sugar solution of the fermented cotton stalk as claimed in claim 1, wherein the combination mechanism (200) comprises a sliding groove (201), a first sliding block (202), a mounting cavity (203), a second sliding block (204), a blocking piece (205), a shifting block (206), a mounting hole (207), a blocking block (208), a blocking block (209), a blocking ring (210), a second spring (211) and a blocking hole (212);
the sliding grooves (201) are inverted T-shaped, the sliding grooves (201) are formed in the upper surface of the top wall of the box body (101), and the number of the sliding grooves (201) is two; the first sliding blocks (202) are fixedly mounted on the lower surface of the box body (101), the first sliding blocks (202) are in an inverted T shape, the length of each first sliding block (202) is larger than the width of the box body (101), the number of the first sliding blocks (202) is two, the two first sliding blocks (202) are matched with the sliding grooves (201), and the two first sliding blocks (202) and the two sliding grooves (201) are respectively located on the same axis;
mounting cavities (203) are formed in two ends of the first sliding block (202), the height of the center of each mounting cavity (203) is larger than that of the two sides of each mounting cavity, second sliding blocks (204) are arranged inside the mounting cavities (203), the number of the second sliding blocks (204) in each mounting cavity (203) is two, blocking pieces (205) are fixedly mounted on one corresponding side of each second sliding block (204), and the blocking pieces are located in the narrow positions of the two sides of each mounting cavity (203); the front surfaces of the two second sliding blocks (204) are fixedly provided with shifting blocks (206), the size of each shifting block (206) is smaller than that of each second sliding block (204), each shifting block (206) protrudes out of the corresponding first sliding block (202), and each first sliding block (202) is provided with a sliding hole only for the corresponding shifting block (206) to slide;
mounting holes (207) are formed in the positions, close to the top ends, of the two sides of the box body (101), and a stop block (208) is fixedly mounted at the outlet of each mounting hole (207); a clamping block (209) is arranged in the mounting hole (207), the clamping block (209) is in an irregular inverted door shape, a blocking ring (210) is fixedly mounted at one end, located in the mounting hole (207), of the clamping block (209), and the diameter of the blocking ring (210) is larger than that formed between the clamping blocks (208); a second spring (211) is fixedly installed at the inner end of the installation hole (207), and the other end of the second spring (211) is fixedly connected with a clamping block (209);
the clamping holes (212) are formed in the two sides of the box body (101), and the clamping holes (212) are respectively located in the two sides of the box body (101) and are close to the bottom end and below the mounting holes.
4. The preservation device for the endophytic bacteria HB1310 of the juglans sigillata produced by hydrolyzing the sugar liquor through the fermented cotton stalks according to claim 1, wherein the freezing mechanism (300) comprises a freezing box (301), a heat insulation plate (302), a semiconductor refrigerating sheet (303), a heat insulation layer (304), a placing hole (305), a third spring (306), a heat insulation interlayer (307), a second handle (308) and a temperature sensor (309);
the freezing box (301) is arranged inside the box body (101), the freezing box (301) is a square box body with an opening at the top, and heat insulation plates (302) are fixedly arranged on the inner surfaces of the freezing box (301); semiconductor refrigerating pieces (303) are fixedly arranged on two sides of the heat insulation plate (302);
insulating layers (304) are fixedly mounted on the other sides of the semiconductor refrigeration sheets (303), the number of the insulating layers (304) is two, a plurality of placing holes (305) are formed in the two insulating layers (304), the top ends of the placing holes (305) penetrate through the insulating layers, and a plurality of third springs (306) are fixedly mounted on the lower surfaces of the inside of the placing holes (305); a heat-insulating interlayer (307) is fixedly arranged between the two heat-insulating layers (304);
a second handle (308) is fixedly arranged on the front surface of the freezing box (301); and a temperature sensor (309) is fixedly mounted at the joint of the interior of the heat-insulating layer (304) and the placing hole (305), and the temperature sensor (309) is connected with the controller (106).
5. The preservation device for the endophytic bacteria HB1310 of the walnut produced by the fermentation cotton stalk hydrolysis sugar liquor according to claim 1, wherein the connecting mechanism (400) comprises a slide rail (401), a third slide block (402), a first limit block (403), a second limit block (404), a movable stop block (405), a pull rod (406), a fourth spring (407), a stop ring (408) and a protection box (409);
sliding rails (401) are fixedly mounted on two sides of the interior of the box body (101), the number of the sliding rails (401) on each side of the box body (101) is two, the sliding rails (401) on the two sides are symmetrically arranged, third sliding blocks (402) are fixedly mounted on two sides of the freezing box (301), and the third sliding blocks (402) are slidably mounted in sliding grooves in the sliding rails (401);
the two sides of the freezing box (301) close to the back are fixedly provided with first limiting blocks (403), and the right side of the freezing box (301) close to the front is also fixedly provided with the first limiting blocks (403); a second limiting block (404) is fixedly arranged on the left side wall of the interior of the box body (101) close to the front outlet;
a pull rod (406) penetrates through the right side wall of the box body (101) and is arranged in a symmetrical position with the second limiting block (404), a movable stopper (405) is fixedly installed at one end, located in the box body (101), of the pull rod (406), and the front face of the movable stopper (405) is a curved surface; a fourth spring (407) is arranged on the part of the outer surface of the pull rod (406) positioned in the box body (101), and the fourth spring (407) is positioned between the movable stop block (405) and the box body (101); a resistance ring (408) is fixedly arranged on the outer surface of the pull rod (406) positioned outside the box body (101); a protective box (409) is fixedly arranged on the outer surface of the box body (101), and the part of the pull rod (406) positioned outside the box body (101) is positioned inside the protective box (409);
in a natural state, one end of the movable stopper (405) far away from the box body (101) is located at the left position in the middle of the first limiting block (403).
6. The preservation device for the endophytic bacteria HB1310 of the walnut produced from the fermented cotton stalk hydrolyzed sugar liquor according to claim 1, wherein the sealing mechanism (500) comprises a pneumatic telescopic rod (501), a sealing cover (502) and a sealing ring (503);
the pneumatic telescopic rod (501) is fixedly arranged on the upper surface of the interior of the box body (101), the position of the pneumatic telescopic rod (501) is determined by the freezing box (301), and the pneumatic telescopic rod (501) is controlled by the controller (106); a sealing cover (502) is fixedly mounted at the bottom end of the pneumatic telescopic rod (501), the bottom end of the pneumatic telescopic rod (501) is positioned at the center of the sealing cover (502), and the size of the bottom surface of the sealing cover (502) is equal to that of the top surface of the freezing box (301); the sealing ring (503) is fixedly arranged on the outer surface of the sealing cover (502), the bottom end of the sealing ring (503) protrudes out of the sealing cover (502), and after the sealing cover (502) is covered on the freezing box (301), the sealing ring (503) is tightly attached to the outer surface of the freezing box (301).
7. The preservation device for the endophytic bacteria HB1310 of the walnut produced by the hydrolyzed sugar solution of the fermented cotton stalk according to claim 1, wherein the storage mechanism (600) comprises a pipe body (601), a pipe holder (602), a pipe cover (603), a sealing gasket (604), an inoculating loop (605) and a lifting loop (606);
the pipe body (601) is a transparent glass pipe, the bottom of the pipe body is conical, a pipe support (602) is installed at the bottom of the pipe body (601) in a threaded mode, and the pipe support (602) is matched with the conical bottom of the pipe body (601); the pipe body (601) is placed on the fourth spring (407) through a pipe bracket;
the top end of the pipe body (601) is in threaded connection with a pipe cover (603), and a sealing gasket (604) is arranged at the joint of the pipe cover (603) and the pipe body (601); an inoculating ring (605) is fixedly mounted at the bottom end of the pipe cover (603), and the inoculating ring (605) penetrates through the sealing gasket (604) and extends to the bottom of the pipe body (601); the upper surface of the pipe cover (603) is fixedly provided with a lifting ring (606).
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| CN202110086917.8A CN112725144B (en) | 2021-01-22 | 2021-01-22 | Preservation device for fermentation cotton stalk hydrolysis sugar liquid oil production walnut endophytic bacteria HB1310 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112680338A (en) * | 2021-01-25 | 2021-04-20 | 塔里木大学 | Inoculation and sampling device for walnut endophytic bacterium HB1310 fermented cotton stalk hydrolyzed sugar liquid oil production fermentation broth |
Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667038A (en) * | 2013-11-30 | 2014-03-26 | 成都欣华源科技有限责任公司 | Strain preservation tube |
| CN105861305A (en) * | 2016-04-21 | 2016-08-17 | 吉林建筑大学 | Soil microorganism molecular biology sample storage device |
| CN107571849A (en) * | 2017-08-23 | 2018-01-12 | 安徽特斯艾尔机电设备有限公司 | A kind of protector in automotive service equipement |
| CN208007571U (en) * | 2018-03-22 | 2018-10-26 | 上海银海圣生物科技有限公司 | A kind of serum stored frozen device |
| CN208531249U (en) * | 2018-08-09 | 2019-02-22 | 山东省滨州畜牧兽医研究院 | A kind of herding vaccine storage box |
| CN208586295U (en) * | 2018-05-18 | 2019-03-08 | 成都德通环境工程有限公司 | A kind of material box for dry fermentation storehouse |
| CN209039456U (en) * | 2018-07-30 | 2019-06-28 | 刘大伟 | A kind of field microbe sampling transport device |
| CN209602515U (en) * | 2018-12-29 | 2019-11-08 | 泌阳县大地菌业有限公司 | A kind of strain moisturizing culture case convenient for stacking |
| CN210259535U (en) * | 2019-07-12 | 2020-04-07 | 河北圣昊光电科技有限公司 | Pure water storage device with automatic sealing function |
| CN210853496U (en) * | 2019-09-25 | 2020-06-26 | 贵州省肿瘤医院 | Endocrinology spot check sample storage device |
| CN211618502U (en) * | 2019-12-31 | 2020-10-02 | 李玉花 | Clinical laboratory uses sample storage device |
| CN211861624U (en) * | 2019-12-12 | 2020-11-06 | 南京三生生物技术股份有限公司 | Freezing device for low-temperature storage of umbilical cord blood |
| CN211946979U (en) * | 2020-02-25 | 2020-11-17 | 宁波明舟生物科技有限公司 | Microorganism storage and preservation device |
| CN111972401A (en) * | 2020-09-03 | 2020-11-24 | 浙江省畜牧技术推广与种畜禽监测总站(浙江省农业机械试验鉴定推广总站) | Semen freezing and sterilizing box for storing Hu sheep semen |
| CN214654888U (en) * | 2021-03-02 | 2021-11-09 | 齐齐哈尔医学院 | Device for freezing and preserving strains |
| CN114246142A (en) * | 2020-09-25 | 2022-03-29 | 洪江市益农益民禽业有限公司 | Poultry is bred with combination formula cage device |
-
2021
- 2021-01-22 CN CN202110086917.8A patent/CN112725144B/en active Active
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667038A (en) * | 2013-11-30 | 2014-03-26 | 成都欣华源科技有限责任公司 | Strain preservation tube |
| CN105861305A (en) * | 2016-04-21 | 2016-08-17 | 吉林建筑大学 | Soil microorganism molecular biology sample storage device |
| CN107571849A (en) * | 2017-08-23 | 2018-01-12 | 安徽特斯艾尔机电设备有限公司 | A kind of protector in automotive service equipement |
| CN208007571U (en) * | 2018-03-22 | 2018-10-26 | 上海银海圣生物科技有限公司 | A kind of serum stored frozen device |
| CN208586295U (en) * | 2018-05-18 | 2019-03-08 | 成都德通环境工程有限公司 | A kind of material box for dry fermentation storehouse |
| CN209039456U (en) * | 2018-07-30 | 2019-06-28 | 刘大伟 | A kind of field microbe sampling transport device |
| CN208531249U (en) * | 2018-08-09 | 2019-02-22 | 山东省滨州畜牧兽医研究院 | A kind of herding vaccine storage box |
| CN209602515U (en) * | 2018-12-29 | 2019-11-08 | 泌阳县大地菌业有限公司 | A kind of strain moisturizing culture case convenient for stacking |
| CN210259535U (en) * | 2019-07-12 | 2020-04-07 | 河北圣昊光电科技有限公司 | Pure water storage device with automatic sealing function |
| CN210853496U (en) * | 2019-09-25 | 2020-06-26 | 贵州省肿瘤医院 | Endocrinology spot check sample storage device |
| CN211861624U (en) * | 2019-12-12 | 2020-11-06 | 南京三生生物技术股份有限公司 | Freezing device for low-temperature storage of umbilical cord blood |
| CN211618502U (en) * | 2019-12-31 | 2020-10-02 | 李玉花 | Clinical laboratory uses sample storage device |
| CN211946979U (en) * | 2020-02-25 | 2020-11-17 | 宁波明舟生物科技有限公司 | Microorganism storage and preservation device |
| CN111972401A (en) * | 2020-09-03 | 2020-11-24 | 浙江省畜牧技术推广与种畜禽监测总站(浙江省农业机械试验鉴定推广总站) | Semen freezing and sterilizing box for storing Hu sheep semen |
| CN114246142A (en) * | 2020-09-25 | 2022-03-29 | 洪江市益农益民禽业有限公司 | Poultry is bred with combination formula cage device |
| CN214654888U (en) * | 2021-03-02 | 2021-11-09 | 齐齐哈尔医学院 | Device for freezing and preserving strains |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112680338A (en) * | 2021-01-25 | 2021-04-20 | 塔里木大学 | Inoculation and sampling device for walnut endophytic bacterium HB1310 fermented cotton stalk hydrolyzed sugar liquid oil production fermentation broth |
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