CN112715950A - Sustained-release probiotic capable of enhancing immunity and preparation method thereof - Google Patents
Sustained-release probiotic capable of enhancing immunity and preparation method thereof Download PDFInfo
- Publication number
- CN112715950A CN112715950A CN202011603121.7A CN202011603121A CN112715950A CN 112715950 A CN112715950 A CN 112715950A CN 202011603121 A CN202011603121 A CN 202011603121A CN 112715950 A CN112715950 A CN 112715950A
- Authority
- CN
- China
- Prior art keywords
- sustained
- enhancing immunity
- controlled release
- maltodextrin
- lactobacillus plantarum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 42
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 42
- 230000036039 immunity Effects 0.000 title claims abstract description 33
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 24
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 22
- 238000013268 sustained release Methods 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000012730 sustained-release form Substances 0.000 title abstract description 4
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 27
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 27
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 22
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 22
- 238000013270 controlled release Methods 0.000 claims abstract description 22
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims abstract description 22
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 22
- PMVSDNDAUGGCCE-TYYBGVCCSA-L Ferrous fumarate Chemical compound [Fe+2].[O-]C(=O)\C=C\C([O-])=O PMVSDNDAUGGCCE-TYYBGVCCSA-L 0.000 claims abstract description 19
- 229960000225 ferrous fumarate Drugs 0.000 claims abstract description 19
- 235000002332 ferrous fumarate Nutrition 0.000 claims abstract description 19
- 239000011773 ferrous fumarate Substances 0.000 claims abstract description 19
- 230000002459 sustained effect Effects 0.000 claims abstract description 18
- WVXRAFOPTSTNLL-NKWVEPMBSA-N 2',3'-dideoxyadenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO)O1 WVXRAFOPTSTNLL-NKWVEPMBSA-N 0.000 claims abstract description 17
- 240000003394 Malpighia glabra Species 0.000 claims abstract description 17
- 235000014837 Malpighia glabra Nutrition 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims abstract description 13
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims abstract description 13
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims abstract description 13
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000006187 pill Substances 0.000 claims abstract description 13
- 239000004925 Acrylic resin Substances 0.000 claims abstract description 11
- 229920000178 Acrylic resin Polymers 0.000 claims abstract description 11
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 11
- 235000019359 magnesium stearate Nutrition 0.000 claims abstract description 11
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 239000008188 pellet Substances 0.000 claims description 31
- 238000002156 mixing Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 13
- 238000005507 spraying Methods 0.000 claims description 12
- 239000002702 enteric coating Substances 0.000 claims description 8
- 238000009505 enteric coating Methods 0.000 claims description 8
- 238000007873 sieving Methods 0.000 claims description 7
- 230000032683 aging Effects 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 24
- 229910052742 iron Inorganic materials 0.000 description 12
- 239000010410 layer Substances 0.000 description 9
- 239000000203 mixture Substances 0.000 description 7
- 102000001554 Hemoglobins Human genes 0.000 description 5
- 108010054147 Hemoglobins Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000013589 supplement Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 208000015710 Iron-Deficiency Anemia Diseases 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000167854 Bourreria succulenta Species 0.000 description 2
- 206010022971 Iron Deficiencies Diseases 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 208000007502 anemia Diseases 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000019693 cherries Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000001872 metatarsal bone Anatomy 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P20/00—Coating of foodstuffs; Coatings therefor; Making laminated, multi-layered, stuffed or hollow foodstuffs
- A23P20/10—Coating with edible coatings, e.g. with oils or fats
- A23P20/15—Apparatus or processes for coating with liquid or semi-liquid products
- A23P20/18—Apparatus or processes for coating with liquid or semi-liquid products by spray-coating, fluidised-bed coating or coating by casting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses sustained and controlled release probiotics for enhancing immunity and a preparation method thereof, wherein the sustained and controlled release probiotics comprise the following raw materials in percentage by weight: 1-6% of lactobacillus plantarum SNK12, 5-20% of acerola powder, 1-6% of ferrous fumarate, 60-80% of maltodextrin, 0.01-0.05% of hydroxypropyl methylcellulose, 0.1-0.6% of pure water, 0.2-1.0% of acrylic resin, 0.01-0.04% of polyethylene glycol, 0.01-0.04% of magnesium stearate and 1.0-3.0% of ethanol. The sustained-release probiotic prepared by the invention can prevent the pill core from being consumed before entering the intestinal tract, and is easier to be quickly absorbed, thereby quickly improving the immunity of the human body.
Description
The technical field is as follows:
the invention relates to the technical field of probiotics, in particular to sustained and controlled release probiotics for enhancing immunity and a preparation method thereof.
Background art:
the term "immunity" is not strange to people, however, few people pay attention to it. Immunity is the body's own defense mechanism, and is the body's ability to recognize and destroy any foreign body (virus, bacteria, etc.) that invades from the outside, to treat aged, damaged, dead, denatured self cells, and to recognize and treat mutant and virally infected cells in the body. However, how to actually and effectively improve the capability is a topic that people pay attention to at present.
One of the methods for enhancing immunity in general is to supplement iron appropriately for the following reasons: iron is one of important essential trace elements of human body, and the human body needs to utilize the iron to generate hemoglobin in red blood cells and transport oxygen. If not enough iron is available, hemoglobin production is reduced, the number of red blood cells in blood is reduced, the volume of the red blood cells is reduced, and a human body cannot obtain enough oxygen, iron deficiency is a common cause of anemia, iron deficiency anemia is anemia caused by iron deficiency in the body and reduced hemoglobin (Hb) synthesis, and the iron deficiency anemia can cause immunity reduction and is easy to cause complications.
However, the existing iron supplement nutriment is usually lost and has limited utilization rate when entering the intestinal tract, and the iron supplement nutriment is relatively slow to supplement, so that the iron supplement nutriment which is easy to absorb, has high utilization rate and is safe to use needs to be developed on the basis.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
The invention content is as follows:
the invention aims to provide sustained and controlled release probiotics for enhancing immunity and a preparation method thereof, thereby overcoming the defects in the prior art.
In order to achieve the aim, the invention provides sustained and controlled release probiotics for enhancing immunity, which comprises the following raw materials in percentage by weight: 1-6% of lactobacillus plantarum SNK12, 5-20% of acerola powder, 1-6% of ferrous fumarate, 60-80% of maltodextrin, 0.01-0.05% of hydroxypropyl methylcellulose, 0.1-0.6% of pure water, 0.2-1.0% of acrylic resin, 0.01-0.04% of polyethylene glycol, 0.01-0.04% of magnesium stearate and 1.0-3.0% of ethanol.
Specifically, the sustained-release probiotic capable of enhancing immunity comprises the following raw materials in percentage by weight: 3.33% of lactobacillus plantarum SNK12, 15% of acerola powder, 2.48% of ferrous fumarate, 76.19% of maltodextrin, 0.02% of hydroxypropyl methylcellulose, 0.48% of pure water, 0.62% of acrylic resin, 0.03% of polyethylene glycol, 0.03% of magnesium stearate and 1.82% of ethanol.
The lactobacillus plantarum SNK12 is 3000CFU/g lactobacillus plantarum.
A preparation method of sustained and controlled release probiotics for enhancing immunity comprises the following steps:
(1) keeping 20% of maltodextrin for standby, mixing the rest maltodextrin with ferrous fumarate for 6 times, adding Lactobacillus plantarum SNK12, continuously mixing, adding acerola powder, and continuously mixing to obtain a mixture;
(2) preparing the mixed material into a soft mixed material by using 20% of reserved maltodextrin, extruding the soft mixed material into a long strip with the length of 0.5-0.8mm by using an extruder with the aperture of a sieving plate being 0.5mm, placing the long strip into a spheronizer to be spheronized, and ageing overnight at room temperature to obtain a pill core for later use;
(3) dispersing hydroxypropyl methylcellulose into pure water, stirring and dissolving until the solution is transparent, and sieving with a 120-mesh nylon sieve to obtain an isolation layer coating solution for later use;
(4) mixing acrylic resin, polyethylene glycol and magnesium stearate uniformly, adding into ethanol, and stirring uniformly to obtain enteric coating solution for later use;
(5) placing the pellet core prepared in the step (2) into a fluidized bed, spraying the isolating layer coating liquid prepared in the step (3) as a spray coating onto the outer surface of the pellet core, and taking out and drying the pellet core after the weight of the pellet core is increased by 0.5% to obtain an isolating pellet core for later use;
(6) placing the isolating pill core into a fluidized bed, spraying the enteric coating liquid prepared in the step (4) as a spray coating onto the outer surface of the isolating pill core, and taking out and drying after the weight of the isolating pill core is increased by 2.5% to obtain the probiotic micro-pellets;
(7) detecting the probiotic pellets and subpackaging into small packages to obtain the finished product of the controlled-release probiotic.
And (3) ageing the pellet core in the step (2) at room temperature for 24-36 h.
And (4) heating and preserving the temperature of the pure water to 40-45 ℃ when the hydroxypropyl methylcellulose in the step (3) is dispersed into the pure water.
And (7) subpackaging the probiotic pellets into small packages of 2 g/bag.
Compared with the prior art, one aspect of the invention has the following beneficial effects:
(1) the pellet core is added with the combination of probiotics, ferrous fumarate and acerola powder, so that the intestinal tract can be improved, the absorption of a human body to nutrients can be promoted, and meanwhile, the natural vitamin C rich in the acerola powder can form a compound with the ferrous fumarate, so that iron is more easily dissolved and quickly absorbed in the intestinal tract, the iron deficiency anemia is improved, and the immunity of the human body is improved;
(2) the invention optimizes the mixture ratio of various raw materials, and has the effect of enhancing immunity from the results of animal experiments, wherein the effects of supplementing iron and enhancing immunity are particularly obvious when the ratio of the lactobacillus plantarum SNK12 to the acerola cherry fruit powder to the ferrous fumarate is 3:10: 2;
(3) the invention prepares the isolating layer outside the pellet core and the enteric coating layer outside the isolating layer, can prevent the pellet core from being consumed before entering the intestinal tract, and can ensure the absorption rate of nutrients by starting to release the probiotics and the iron nutrient after the enteric coating layer and the isolating layer are dissolved after the probiotic pellets enter the intestinal tract, thereby quickly improving the immunity of a human body.
Description of the drawings:
FIG. 1: statistical table of the effects of the samples of examples 1-7 of the present invention administered to female mice continuously.
The specific implementation mode is as follows:
the following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Throughout the specification and claims, unless explicitly stated otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or component but not the exclusion of any other element or component.
Example 1:
a sustained and controlled release probiotic for enhancing immunity comprises the following raw materials by weight percent: 3.33% of lactobacillus plantarum SNK12, 15% of acerola powder, 2.48% of ferrous fumarate, 76.19% of maltodextrin, 0.02% of hydroxypropyl methylcellulose, 0.48% of pure water, 0.62% of acrylic resin, 0.03% of polyethylene glycol, 0.03% of magnesium stearate and 1.82% of ethanol, wherein the lactobacillus plantarum SNK12 is 3000CFU/g of lactobacillus plantarum.
A preparation method of sustained and controlled release probiotics for enhancing immunity comprises the following steps:
(1) keeping 20% of maltodextrin for standby, mixing the rest maltodextrin with ferrous fumarate for 6 times, adding Lactobacillus plantarum SNK12, continuously mixing, adding acerola powder, and continuously mixing to obtain a mixture;
(2) preparing the mixed material into a soft mixed material by using 20% of reserved maltodextrin, extruding the soft mixed material into a strip with the length of 0.5-0.8mm by using an extruder with the aperture of a sieving plate being 0.5mm, placing the strip into a spheronizer to be spheronized, and ageing the strip overnight for 30 hours at room temperature to obtain a pill core for later use;
(3) dispersing hydroxypropyl methylcellulose into pure water at 40 ℃, stirring and dissolving until the solution is transparent, and sieving with a 120-mesh nylon sieve to obtain an isolation layer coating solution for later use;
(4) mixing acrylic resin, polyethylene glycol and magnesium stearate uniformly, adding into ethanol, and stirring uniformly to obtain enteric coating solution for later use;
(5) placing the pellet core prepared in the step (2) into a fluidized bed, spraying the isolating layer coating liquid prepared in the step (3) as a spray coating onto the outer surface of the pellet core, and taking out and drying the pellet core after the weight of the pellet core is increased by 0.5% to obtain an isolating pellet core for later use;
(6) placing the isolating pill core into a fluidized bed, spraying the enteric coating liquid prepared in the step (4) as a spray coating onto the outer surface of the isolating pill core, and taking out and drying after the weight of the isolating pill core is increased by 2.5% to obtain the probiotic micro-pellets;
(7) detecting the probiotic pellets and subpackaging into small packages of 2 g/bag to obtain the finished product of the controlled-release probiotic. Example 2:
different from the embodiment 1, the sustained and controlled release probiotics for enhancing the immunity comprises the following raw materials in percentage by weight: 5.35% of lactobacillus plantarum SNK12, 20% of acerola powder, 3.52% of ferrous fumarate, 68.13% of maltodextrin, 0.02% of hydroxypropyl methylcellulose, 0.48% of pure water, 0.62% of acrylic resin, 0.03% of polyethylene glycol, 0.03% of magnesium stearate and 1.82% of ethanol, wherein 3000CFU/g of lactobacillus plantarum is selected as lactobacillus plantarum SNK 12.
Example 3:
different from the embodiment 1, the sustained and controlled release probiotics for enhancing the immunity comprises the following raw materials in percentage by weight: 1.5% of lactobacillus plantarum SNK12, 14% of acerola powder, 2.0% of ferrous fumarate, 79.48% of maltodextrin, 0.02% of hydroxypropyl methylcellulose, 0.48% of pure water, 0.64% of acrylic resin, 0.03% of polyethylene glycol, 0.03% of magnesium stearate and 1.82% of ethanol, wherein 3000CFU/g of lactobacillus plantarum is selected for lactobacillus plantarum SNK 12.
Example 4:
the probiotics for enhancing immunity comprises the following raw materials in percentage by weight: 4.33% of lactobacillus plantarum SNK12, 16% of acerola powder, 3.48% of ferrous fumarate and 76.19% of maltodextrin, wherein 3000CFU/g of lactobacillus plantarum is selected as lactobacillus plantarum SNK 12.
A preparation method of sustained and controlled release probiotics for enhancing immunity comprises the following steps:
(1) keeping 20% of maltodextrin for standby, mixing the rest maltodextrin with ferrous fumarate for 6 times, adding Lactobacillus plantarum SNK12, continuously mixing, adding acerola powder, and continuously mixing to obtain a mixture;
(2) preparing the mixed material into a soft mixed material by using 20% of reserved maltodextrin, extruding the soft mixed material into a strip with the length of 0.5-0.8mm by using an extruder with the aperture of a sieving plate being 0.5mm, placing the strip into a spheronizer to be spheronized, and ageing the strip overnight for 30 hours at room temperature to prepare the probiotic micro-pellets;
(3) detecting the probiotic pellets and subpackaging into small packages of 2 g/bag.
Example 5:
0.0666g of lactobacillus plantarum and 0.0496g of ferrous fumarate which are the same as those in the small package in the amount of 0.0666g of the sample in the example 2 g/bag are weighed as a sample reagent according to the mixture ratio of the example 1.
Example 6:
0.0496g of ferrous fumarate with the same amount as that of the ferrous fumarate in a small package in the example 2 g/bag is weighed as a sample reagent according to the mixture ratio of the example 1.
Example 7:
1.5238g of maltodextrin in the same amount as that in the small package of example 2 g/bag is weighed as a sample reagent according to the mixture ratio of example 1.
The test subjects were divided into 7 groups of 8 mice each of which was fed with the sample reagent of examples 1 to 7, and the administration effect was counted after 24 days of continuous feeding, and the phagocytic index (a), the degree of swelling of metatarsal edema (mm), the difference in lymphocyte proliferation ability OD, the median value of blood solubility (HC50), and the hemoglobin (Hb) index were evaluated, as shown in fig. 1.
The test results show that the samples prepared by the controlled-release probiotics and the preparation method thereof have obvious effects of supplementing iron and improving immunity, wherein the content of hemoglobin shows that the probiotics and acerola cherry can effectively promote the absorption of iron; the invention can effectively improve the immunity according to various indexes of the immunity.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (7)
1. A sustained and controlled release probiotic for enhancing immunity, characterized by: comprises the following raw materials in percentage by weight: 1-6% of lactobacillus plantarum SNK12, 5-20% of acerola powder, 1-6% of ferrous fumarate, 60-80% of maltodextrin, 0.01-0.05% of hydroxypropyl methylcellulose, 0.1-0.6% of pure water, 0.2-1.0% of acrylic resin, 0.01-0.04% of polyethylene glycol, 0.01-0.04% of magnesium stearate and 1.0-3.0% of ethanol.
2. The sustained and controlled release probiotic bacteria for enhancing immunity according to claim 1, characterized in that: comprises the following raw materials in percentage by weight: 3.33% of lactobacillus plantarum SNK12, 15% of acerola powder, 2.48% of ferrous fumarate, 76.19% of maltodextrin, 0.02% of hydroxypropyl methylcellulose, 0.48% of pure water, 0.62% of acrylic resin, 0.03% of polyethylene glycol, 0.03% of magnesium stearate and 1.82% of ethanol.
3. The sustained and controlled release probiotic bacteria for enhancing immunity according to claim 1, characterized in that: the lactobacillus plantarum SNK12 is 3000CFU/g lactobacillus plantarum.
4. A preparation method of sustained and controlled release probiotics for enhancing immunity is characterized by comprising the following steps: the method comprises the following steps:
(1) leaving 20% of maltodextrin for standby, mixing the rest maltodextrin with ferrous fumarate according to 6 times, adding lactobacillus plantarum SNK12 for continuous mixing, adding acerola powder for continuous mixing to obtain a mixed material;
(2) preparing the mixed material into a soft mixed material by using 20% of reserved maltodextrin, extruding the soft mixed material into a strip with the length of 0.5-0.8mm by using an extruder with the aperture of a sieving plate being 0.5mm, placing the strip into a rounding machine for rounding, and ageing overnight at room temperature to obtain a pill core for later use;
(3) dispersing hydroxypropyl methylcellulose into pure water, stirring and dissolving until the solution is transparent, and sieving with a 120-mesh nylon sieve to obtain an isolation layer coating solution for later use;
(4) mixing acrylic resin, polyethylene glycol and magnesium stearate uniformly, adding into ethanol, and stirring uniformly to obtain enteric coating solution for later use;
(5) placing the pellet core prepared in the step (2) into a fluidized bed, spraying the isolating layer coating liquid prepared in the step (3) as a spray coating onto the outer surface of the pellet core, and taking out and drying the pellet core after the weight of the pellet core is increased by 0.5% to obtain an isolating pellet core for later use;
(6) placing the isolating pill core into a fluidized bed, spraying the enteric coating liquid prepared in the step (4) as a spray coating onto the outer surface of the isolating pill core, and taking out and drying after the weight of the isolating pill core is increased by 2.5% to obtain the probiotic micro-pellets;
(7) detecting the probiotic pellets and subpackaging into small packages to obtain the finished product of the controlled-release probiotic.
5. The preparation method of the sustained and controlled release probiotics for enhancing immunity according to claim 4, is characterized in that: and (3) ageing the pellet core in the step (2) at room temperature for 24-36 h.
6. The preparation method of the sustained and controlled release probiotics for enhancing immunity according to claim 4, is characterized in that: and (4) heating and preserving the temperature of the pure water to 40-45 ℃ when the hydroxypropyl methylcellulose in the step (3) is dispersed into the pure water.
7. The preparation method of the sustained and controlled release probiotics for enhancing immunity according to claim 4, is characterized in that: and (7) subpackaging the probiotic pellets into small packages of 2 g/bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011603121.7A CN112715950A (en) | 2020-12-30 | 2020-12-30 | Sustained-release probiotic capable of enhancing immunity and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011603121.7A CN112715950A (en) | 2020-12-30 | 2020-12-30 | Sustained-release probiotic capable of enhancing immunity and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112715950A true CN112715950A (en) | 2021-04-30 |
Family
ID=75611680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011603121.7A Pending CN112715950A (en) | 2020-12-30 | 2020-12-30 | Sustained-release probiotic capable of enhancing immunity and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112715950A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102961338A (en) * | 2012-12-07 | 2013-03-13 | 青岛黄海制药有限责任公司 | Polyferose controlled-release pellet and preparation method thereof |
| CN109170472A (en) * | 2018-10-16 | 2019-01-11 | 鲲鱼健康药业江苏有限公司 | Direct drinking type acerola concentrate solid beverage |
| CN109329419A (en) * | 2018-08-28 | 2019-02-15 | 吉林标普生物科技有限公司 | A kind of functional probiotics solid beverage and preparation method thereof for being able to maintain that intestinal health and improving immunity |
| CN110882281A (en) * | 2019-11-30 | 2020-03-17 | 江苏艾兰得营养品有限公司 | Probiotic enteric-coated tablet and preparation method thereof |
-
2020
- 2020-12-30 CN CN202011603121.7A patent/CN112715950A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102961338A (en) * | 2012-12-07 | 2013-03-13 | 青岛黄海制药有限责任公司 | Polyferose controlled-release pellet and preparation method thereof |
| CN109329419A (en) * | 2018-08-28 | 2019-02-15 | 吉林标普生物科技有限公司 | A kind of functional probiotics solid beverage and preparation method thereof for being able to maintain that intestinal health and improving immunity |
| CN109170472A (en) * | 2018-10-16 | 2019-01-11 | 鲲鱼健康药业江苏有限公司 | Direct drinking type acerola concentrate solid beverage |
| CN110882281A (en) * | 2019-11-30 | 2020-03-17 | 江苏艾兰得营养品有限公司 | Probiotic enteric-coated tablet and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102038691B (en) | Composite preparation of vitamins and amino acids and preparation method thereof | |
| CN103431134A (en) | Tablet candy containing prebiotics and preparation method thereof | |
| CN102068454B (en) | Slow-release regulating agent for preventing rumen acidosis and preparation method thereof | |
| CN101491293A (en) | Natural feed additive and preparation method thereof | |
| CN109673847B (en) | Preparation process of intestinal slow-release acidifier | |
| CN108095122A (en) | Lutein ester blueberry haematococcus piece and preparation method thereof | |
| CN112715950A (en) | Sustained-release probiotic capable of enhancing immunity and preparation method thereof | |
| CN106902150A (en) | Co-Q10 purple perilla soft capsule prescription and preparation method | |
| TW202033205A (en) | Astaxanthin complex and the method thereof | |
| CN103271940B (en) | Compound metal ion replenisher and preparation method thereof | |
| CN106923018A (en) | A kind of fermented pig feed addictive and preparation method thereof | |
| CN103907785B (en) | A kind of atherosclerotic slaking breast piglet biological feedstuff and preparation method thereof | |
| CN107129430B (en) | Method for synthesizing medium-chain triglyceride of bulk drugs | |
| CN111213790A (en) | High-efficiency feed additive | |
| CN101874573A (en) | Calcium supplement pet dog food and preparation method thereof | |
| CN109043545A (en) | A kind of compound bio element nutritional preparation and preparation method thereof, application | |
| CN114317367A (en) | Probiotic composition, application of composition and auxiliary anti-alcohol preparation | |
| CN109699947A (en) | A kind of anti-oxidant egg piece and its processing method | |
| CN108741104B (en) | Easily-absorbed composite whey protein powder and preparation method thereof | |
| CN111423520A (en) | Processing technology of modified cereal starch with high digestion resistance | |
| CN102048027A (en) | Food yeast-containing nutritional composition for animals and preparation method thereof | |
| WO2022032804A1 (en) | Compound amino acid enteric-coated tablet for dogs and preparation method therefor | |
| CN102614143B (en) | High-stability vitamin C tablet and preparing process thereof | |
| CN110463904A (en) | A kind of wheat bran compound nutritional chewable tablets and its processing method | |
| CN104522681B (en) | A kind of vitamin A absorption accelerator and corresponding vitamin A supplement thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210430 |