CN1127139A - Preparation of heterogenic animal high immune serum against duck pest and viral hepatitis - Google Patents
Preparation of heterogenic animal high immune serum against duck pest and viral hepatitis Download PDFInfo
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- CN1127139A CN1127139A CN95112185A CN95112185A CN1127139A CN 1127139 A CN1127139 A CN 1127139A CN 95112185 A CN95112185 A CN 95112185A CN 95112185 A CN95112185 A CN 95112185A CN 1127139 A CN1127139 A CN 1127139A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invented method consists of preparation of duck plague virus seed, preparation of virus seed of duck viral hepatitis, preparation of first immunologic antigen, preparation of second immunologic antigen and preparation of heterogenic animal serum. The high immune serum made by said method has obvious curative effect for preventing and curing duck plague and duck viral hepatitis the preventing rate is 95% and cure rate is 87%.
Description
The present invention relates to a kind of preparation method of animal serum, be specially the preparation method of the allos animal serum of a kind of anti-duck pestilence and viral hepatitis.
Duck pestilence and duck viral hepatitis all are due to the anxious deadly infectious disease that is caused by virus, involve a wide range of knowledge, sickness rate height, mortality rate be all more than 90%, bring great economic loss for the unit or the individual that raise duck fowl, simultaneously, because the duck fowl that disease is died is dealt with improperly, often cause stretching of virus, produce bigger disastrous effect.At present, do not find a kind of specific medicament that is exclusively used in treatment duck pestilence and duck viral hepatitis in the society as yet, this is that duck poultry raising unit or individual feel pain in the neck most.
Task of the present invention is to provide a kind of preparation method that is exclusively used in the specific medicament of prevention and treatment duck pestilence and duck viral hepatitis, the preparation method of the allos animal serum of promptly anti-duck pestilence and viral hepatitis.
The preparation method of the allos animal serum of anti-duck pestilence of the present invention and duck viral hepatitis comprises: the producing of the producing of duck pestilence kind poison, duck viral hepatitis kind poison, exempt from antigenicly to produce, two exempt from antigenicly to produce, the producing of allos animal serum, and its concrete preparation method is:
Producing of a, duck pestilence kind poison:
1, under aseptic condition, gets because of suffering from the duck internal organs (comprising the heart, liver, spleen, lung, brain) that duck pestilence is died of illness and died, put into tissue mashing machine and smash to pieces;
2, be 100 to calculate by gross weight, getting and accounting for total amount be the duck tissue smashed to pieces of the quilt of 10-20% with accounting for gross weight is that the concentration of 80-90% is that 0.8% normal saline mixes formation line and staff control suspension;
3, line and staff control's suspension is put into centrifuge, after under 4000 rev/mins of conditions centrifugal 20-40 minute, get its supernatant;
4, thereon in the clear liquid, promptly form duck pestilence kind poison after the proportioning that adds 2000 unit penicillins and 2000 unit streptomycins by every milliliter of supernatant adds penicillin and streptomycin, and put it in the refrigerator and under-4 ℃ of conditions, be in store for;
Producing of b, duck viral hepatitis kind poison
Under aseptic condition, get because of suffering from the duck internal organs (comprising the heart, liver, spleen, lung, brain) of duck viral hepatitis death, take promptly to obtain duck viral hepatitis kind poison, and put it in the refrigerator and under-4 ℃ of conditions, be in store for the identical method of the described method of a.
C, one exempts from antigenic producing
1, the duck pestilence kind poison of method described in a being produced, put into concentration and be 100 times of 0.8% normal saline dilutions, form duck pestilence kind poison diluent, duck pestilence kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 9-12 age in days, be chosen at the duck embryo that has feature death such as hemorrhage, edema between 48-120 hour after the inoculation, get its allantoic fluid, in every milliliter of allantoic fluid, add and promptly form one behind the streptomycin of the toximycin of 2000 units and 2000 units and exempt from duck pestilence antigen, and put it in the refrigerator and under-4 ℃ of conditions, be in store for.
2, it is 100 times of 0.8% normal saline dilutions that the duck viral hepatitis kind poison that method described in a is produced is put into concentration, form duck viral hepatitis kind poison diluent, duck viral hepatitis kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 9-12 age in days, be chosen at after the inoculation have between 48-120 hour hemorrhage, the duck embryo of feature death such as edema, get its allantoic fluid, in its every milliliter allantoic fluid, add and promptly form one behind the streptomycin of the penicillin of 2000 units and 2000 units and exempt from duck viral hepatitis antigen, and put it in the refrigerator and under-4 ℃ of conditions, be in store for.
D, two exempts from antigenic producing
Getting one exempts from the antigenic preparation method behind inoculation duck pestilence kind poison dead duck idiosome and puts into tissue mashing machine and smash to pieces, by gross weight is 100 calculating, getting and accounting for gross weight be the duck idiosome tissue smashed to pieces of the quilt of 10-20% with accounting for gross weight is that the concentration of 80-90% is that 0.8% normal saline mixes, form line and staff control's suspension, line and staff control's suspension is put into centrifuge, after under 4000 rev/mins of conditions centrifugal 20-40 minute, get its supernatant, the proportioning adding penicillin and the streptomycin that add 2000 unit penicillins and 2000 unit streptomycins thereon in the clear liquid by every milliliter of supernatant, and add formalin solution by 0.4% proportioning of gross weight and make inactivated vaccine, promptly become two and exempt from duck pestilence antigen, and at room temperature preserve standby.Take one to exempt from the antigenic preparation method to poison the duck idiosome of dying with poison and can obtain two with same way and exempt from duck viral hepatitis antigen, and at room temperature preserve standby through inoculation duck viral hepatitis kind.
Producing of e, allos animal serum
1, allos animal bigeminy hyper-immune serum produces
The healthy anosis allos animal of choosing is (as horse, cattle, pig, sheep, rabbit etc.), by selected allos the weight of animals per 10 kilograms at its cervical region, abdominal part both sides multiple spot (5-10 point) subcutaneous injection one is exempted from duck pestilence antigen and one and is exempted from each 1 milliliter of duck viral hepatitis antigen, carrying out two after 7 days exempts from, allos animal after promptly exempting to one, inject 1 milliliter two at its subcutaneous multiple spot (5-10 point) and exempt from duck pestilence antigen and 1 milliliter two and exempt from duck viral hepatitis antigen for per 10 kilograms by its body weight, carrying out three again after 7 days exempts from, allos animal after promptly exempting to two, by its body weight per 10 kilograms at neck, 3.5 milliliter one of abdominal part multiple spot (5-10 point) injection exempted from duck pestilence antigen and 3.5 milliliter one and exempted from duck viral hepatitis antigen.Three exempt from the injection back every blood sampling in 5 days once, carrying out antibody titer detects, after 30 days when antibody titer 1: 16-1: during 128 scopes, this allos animal is taken a blood sample under aseptic condition, the blood that to be gathered is put into sterile chamber and is carried out drawing its serum after the natural separation, after in its serum, adding 2000 unit penicillins and 2000 unit streptomycins by every milliliter of serum, promptly become the bigeminy hyper-immune serum behind the 1/10000 amount adding thimerosal by its gross weight again, to its hyper-immune serum packing, seal, and place under one 18 ℃ of conditions and preserve.
2, the single hyper-immune serum of allos animal produces
Go up single injection duck pestilence antigen or duck viral hepatitis antigen the anosis allos animal of health (as horse, cattle, pig, sheep, rabbit etc.), other method is fully identical with allos animal bigeminy hyper-immune serum preparation method, can produce the hyper-immune serum of simple function respectively, promptly anti-duck pestilence allos animal hyper-immune serum or anti-duck viral hepatitis allos animal hyper-immune serum.
Below will be described further the preparation method of the allos animal hyper-immune serum of anti-duck pestilence of the present invention and duck viral hepatitis according to embodiment:
Embodiment 1
Under aseptic condition, get because of suffering from the die of illness duck internal organs (comprising the heart, liver, spleen, lung, brain) of dying of duck pestilence and put into tissue mashing machine and smash to pieces, get the duck tissue that 10% the quilt that accounts for gross weight is smashed to pieces, 90% concentration that accounts for gross weight is 0.8% normal saline mixing, form line and staff control's suspension, and put it in the centrifuge under 4000 rev/mins of conditions centrifugal 20 minutes, get its supernatant, promptly form duck pestilence kind poison after in supernatant, adding 2000 unit penicillins and 2000 unit streptomycins by every milliliter of supernatant.Under aseptic condition, get because of adopting said method can obtain duck viral hepatitis kind poison behind the internal organs of suffering from the dead duck of duck viral hepatitis.It is 100 times of 0.8% normal saline dilutions that duck pestilence kind poison or duck viral hepatitis kind poison are put into concentration, form duck pestilence or duck viral hepatitis kind poison diluent, in the duck embryo allantois that is inoculated in 9 ages in days with the duck pestilence or the duck viral hepatitis diluent of 0.1 milliliter of dosage, be chosen at the duck embryo of feature death such as 48 hours hemorrhage, edema after the inoculation, get its allantoic fluid, in every milliliter of allantoic fluid, add the penicillin of 2000 units and the streptomycin of 2000 units, promptly form one and exempt from duck pestilence antigen or duck viral hepatitis antigen.Getting behind inoculation duck pestilence kind poison or the duck viral hepatitis kind poison dead duck idiosome puts into tissue mashing machine and smashs to pieces, getting and accounting for total amount be the duck idiosome tissue smashed to pieces of 10% quilt with accounting for total amount is that 90% concentration is that 0.8% normal saline mixes, form line and staff control's suspension, and put it in the centrifuge, after under 4000 rev/mins of conditions centrifugal 20 minutes, get its supernatant, add 2000 unit penicillins and 2000 unit streptomycins by every milliliter in the clear liquid thereon, and add formalin solution by 0.4% proportioning of total amount and make inactivated vaccine, promptly become two and exempt from duck pestilence or duck viral hepatitis antigen.The healthy anosis pig of choosing, exempt from each 1 milliliter of duck pestilence and duck viral hepatitis antigen at 5 subcutaneous injections one in its abdominal part both sides for per 10 kilograms by its body weight, after 7 days to its by body weight per 10 kilograms subcutaneous 5 inject two exempt from duck pestilence and two exempt from duck viral hepatitis antigen each 1 milliliter, again after 7 days to its body weight per 10 kilograms subcutaneous 5 the injection one exempt from duck pestilence antigen and one exempt from duck viral hepatitis antigen each 3.5 milliliters, the injection back is once carried out antibody titer every blood sampling in 5 days and is detected, after 30 days when antibody titer during at 1: 16, this allos animal is taken a blood sample under aseptic condition, the blood that to be gathered is put into and is drawn its serum after sterile chamber is carried out natural separation, adds behind 2000 unit penicillins and the 2000 unit streptomycins by every milliliter of serum to be bigeminy hyper-immune serum of the present invention after 1/10000 amount by its gross weight adds thimerosal in serum again.
Embodiment 2
Under aseptic condition, get because of suffering from the die of illness duck internal organs (comprising the heart, liver, spleen, lung, brain) of dying of duck pestilence and put into tissue mashing machine and smash to pieces, get the duck tissue that 15% the quilt that accounts for gross weight is smashed to pieces, 85% concentration that accounts for gross weight is 0.8% normal saline mixing, form line and staff control's suspension, and put it in the centrifuge under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, promptly form duck pestilence kind poison after in supernatant, adding 2000 unit penicillins and 2000 unit streptomycins by every milliliter of supernatant.Under aseptic condition, get because of adopting said method can obtain duck viral hepatitis kind poison behind the internal organs of suffering from the dead duck of duck viral hepatitis.It is 100 times of 0.8% normal saline dilutions that duck pestilence kind poison or duck viral hepatitis kind poison are put into concentration, form duck pestilence or duck viral hepatitis kind poison diluent, in the duck embryo allantois that is inoculated in 11 ages in days with the duck pestilence or the duck viral hepatitis diluent of 0.1 milliliter of dosage, be chosen at the duck embryo of feature death such as 80 hours hemorrhage, edema after the inoculation, get its allantoic fluid, in every milliliter of allantoic fluid, add the penicillin of 2000 units and the streptomycin of 2000 units, promptly form one and exempt from duck pestilence or duck viral hepatitis antigen.Getting inoculation duck pestilence kind poison or duck viral hepatitis kind poisons the duck idiosome of dying with poison and puts into tissue mashing machine and smash to pieces, getting and accounting for total amount be the duck idiosome tissue smashed to pieces of 15% quilt with accounting for total amount is that 85% concentration is that 0.8% normal saline mixes, form line and staff control's suspension, and put it in the centrifuge, after under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, add 2000 unit penicillins and 2000 unit streptomycins by every milliliter in the clear liquid thereon, and add formalin solution by 0.4% proportioning of total amount and make inactivated vaccine, promptly become two and exempt from duck pestilence or duck viral hepatitis antigen.The healthy anosis cattle of choosing, exempt from each 1 milliliter of duck pestilence and duck viral hepatitis antigen at 8 subcutaneous injections one in its cervical region both sides for per 10 kilograms by its body weight, after 7 days to its by body weight per 10 kilograms subcutaneous 8 inject two exempt from duck pestilence and two exempt from duck viral hepatitis antigen each 1 milliliter, again after 7 days to its body weight per 10 kilograms subcutaneous 5 the injection one exempt from duck pestilence antigen and one exempt from duck viral hepatitis antigen each 3.5 milliliters, the injection back is once carried out antibody titer every blood sampling in 5 days and is detected, after 30 days when antibody titer during at 1: 64, this allos animal is taken a blood sample under aseptic condition, the blood that to be gathered is put into and is drawn its serum after sterile chamber is carried out natural separation, adds behind 2000 unit penicillins and the 2000 unit streptomycins by every milliliter of serum to be bigeminy hyper-immune serum of the present invention after 1/10000 amount by its gross weight adds thimerosal in serum again.
Embodiment 3
Under aseptic condition, get because of suffering from the die of illness duck internal organs (comprising the heart, liver, spleen, lung, brain) of dying of duck pestilence and put into tissue mashing machine and smash to pieces, get the duck tissue that 20% the quilt that accounts for gross weight is smashed to pieces, 80% concentration that accounts for gross weight is 0.8% normal saline mixing, form line and staff control's suspension, and put it in the centrifuge under 4000 rev/mins of conditions centrifugal 40 minutes, get its supernatant, promptly form duck pestilence kind poison after in supernatant, adding 2000 unit penicillins and 2000 unit streptomycins by every milliliter of supernatant.Under aseptic condition, get because of adopting said method can obtain duck viral hepatitis kind poison behind the internal organs of suffering from the dead duck of duck viral hepatitis.It is 100 times of 0.8% normal saline dilutions that duck pestilence kind poison or duck viral hepatitis kind poison are put into concentration, form duck pestilence or duck viral hepatitis kind poison diluent, in the duck embryo allantois that is inoculated in 12 ages in days with the duck pestilence or the duck viral hepatitis diluent of 0.1 milliliter of dosage, be chosen at the duck embryo of feature death such as 48 hours hemorrhage, edema after the inoculation, get its allantoic fluid, in every milliliter of allantoic fluid, add the penicillin of 2000 units and the streptomycin of 2000 units, promptly form one and exempt from duck pestilence or duck viral hepatitis antigen.Getting inoculation duck pestilence kind poison or duck viral hepatitis kind poisons the duck idiosome of dying with poison and puts into tissue mashing machine and smash to pieces, getting and accounting for total amount be the duck idiosome tissue smashed to pieces of 20% quilt with accounting for total amount is that 80% concentration is that 0.8% normal saline mixes, form line and staff control's suspension, and it puts into centrifuge, after under 4000 rev/mins of conditions centrifugal 40 minutes, get its supernatant, add 2000 unit penicillins and 2000 unit streptomycins by every milliliter in the clear liquid thereon, and add formalin solution by 0.4% proportioning of total amount and make inactivated vaccine, promptly become two and exempt from duck pestilence or duck viral hepatitis antigen.The healthy anosis sheep of choosing, exempt from each 1 milliliter of duck pestilence and duck viral hepatitis antigen at 10 subcutaneous injections one in its abdominal part both sides for per 10 kilograms by its body weight, after 7 days to its by body weight per 10 kilograms subcutaneous 10 inject two exempt from duck pestilence and two exempt from duck viral hepatitis antigen each 1 milliliter, again after 7 days to its body weight per 10 kilograms subcutaneous 10 the injection one exempt from duck pestilence antigen and one exempt from duck viral hepatitis antigen each 3.5 milliliters, the injection back is once carried out antibody titer every blood sampling in 5 days and is detected, after 30 days when antibody titer during at 1: 128, this allos animal is taken a blood sample under aseptic condition, the blood that to be gathered is put into and is drawn its serum after sterile chamber is carried out natural separation, adds behind 2000 unit penicillins and the 2000 unit streptomycins by every milliliter of serum to be bigeminy hyper-immune serum of the present invention after 1/10000 amount by its gross weight adds thimerosal in serum again.
Embodiment 2 is most preferred embodiment of the present invention.
The using method of the allos animal hyper-immune serum of anti-duck pestilence of the present invention and duck viral hepatitis is:
1, as preventing duck pestilence and duck viral hepatitis simultaneously, every Corium Anas domestica is injected 0.5-1 milliliter bigeminy hyper-immune serum down;
2, as single prevention duck pestilence or duck viral hepatitis, every Corium Anas domestica is injected the anti-duck pestilence hyper-immune serum or the duck viral hepatitis hyper-immune serum of 0.25-0.5 milliliter down;
3, as treating duck pestilence and duck viral hepatitis simultaneously, every Corium Anas domestica is injected 1-3 milliliter bigeminy hyper-immune serum down;
4, as single therapy duck pestilence or duck viral hepatitis, every Corium Anas domestica is injected the anti-duck pestilence hyper-immune serum or the duck viral hepatitis hyper-immune serum of 0.5-1.5 milliliter down.
Allos animal height with anti-duck plague of the present invention and duck virus hepatitis is exempted from blood The hyper-immune serum that clear preparation method makes is for prevention and treatment duck plague and duck viral Hepatitis has significant curative effect, and its prevention rate is 95%, and cure rate 87% can have The realization task of the present invention of effect is for society provides a kind of special-purpose prevention and treatment duck plague Preparation method with the specific medicament of duck virus hepatitis.
Claims (2)
1, the preparation method of the allos animal hyper-immune serum of a kind of anti-duck pestilence and duck viral hepatitis, comprise that the producing of the producing of duck pestilence kind poison, duck viral hepatitis kind poison, exempt from antigenicly to produce, two exempt from antigenic producing, producing of allos animal serum is characterized in that:
Producing of a, duck pestilence kind poison:
(1) under aseptic condition, gets because of suffering from the duck internal organs (comprising the heart, liver, spleen, lung, brain) that duck pestilence is died of illness and died, put into tissue mashing machine and smash to pieces;
(2) be 100 to calculate by gross weight, getting and accounting for total amount be the duck tissue smashed to pieces of the quilt of 10-20% with accounting for gross weight is that the concentration of 80-90% is that 0.8% normal saline mixes formation line and staff control suspension;
(3) line and staff control's suspension is put into centrifuge, after under 4000 rev/mins of conditions centrifugal 20-40 minute, get its supernatant;
(4) thereon in the clear liquid, promptly form duck pestilence kind poison after the proportioning that adds 2000 unit penicillins and 2000 unit streptomycins by every milliliter of supernatant adds penicillin and streptomycin, and put it in the refrigerator and under-4 ℃ of conditions, be in store for;
Producing of b, duck viral hepatitis kind poison
Under aseptic condition, get because of suffering from the duck internal organs (comprising the heart, liver, spleen, lung, brain) of duck viral hepatitis death, take promptly to obtain duck viral hepatitis kind poison, and put it in the refrigerator and under-4 ℃ of conditions, be in store for the identical method of the described method of a;
C, one exempts from antigenic producing
(1) the duck pestilence kind poison of method described in a being produced, put into concentration and be 100 times of 0.8% normal saline dilutions, form duck pestilence kind poison diluent, duck pestilence kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 9-12 age in days, be chosen at the duck embryo that has feature death such as hemorrhage, edema between 48-120 hour after the inoculation, get its allantoic fluid, in every milliliter of allantoic fluid, add and promptly form one behind the streptomycin of the toximycin of 2000 units and 2000 units and exempt from duck pestilence antigen, and put it in the refrigerator and under-4 ℃ of conditions, be in store for;
(2) it is 100 times of 0.8% normal saline dilutions that the duck viral hepatitis kind poison of method described in a being produced is put into concentration, form duck viral hepatitis kind poison diluent, duck viral hepatitis kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 9-12 age in days, be chosen at after the inoculation have between 48-120 hour hemorrhage, the duck embryo of feature death such as edema, get its allantoic fluid, in its every milliliter allantoic fluid, add and promptly form one behind the streptomycin of the penicillin of 2000 units and 2000 units and exempt from duck viral hepatitis antigen, and put it in the refrigerator and under-4 ℃ of conditions, be in store for;
D, two exempts from antigenic producing
Getting one exempts from the antigenic preparation method behind inoculation duck pestilence kind poison dead duck idiosome and puts into tissue mashing machine and smash to pieces, by gross weight is 100 calculating, getting and accounting for gross weight be the duck idiosome tissue smashed to pieces of the quilt of 10-20% with accounting for gross weight is that the concentration of 80-90% is that 0.8% normal saline mixes, form line and staff control's suspension, line and staff control's suspension is put into centrifuge, after under 4000 rev/mins of conditions centrifugal 20-40 minute, get its supernatant, the proportioning adding penicillin and the streptomycin that add 2000 unit penicillins and 2000 unit streptomycins thereon in the clear liquid by every milliliter of supernatant, and add formalin solution by 0.4% proportioning of gross weight and make inactivated vaccine, promptly become two and exempt from duck pestilence antigen, and at room temperature preserve standby.Take one to exempt from the antigenic preparation method to poison the duck idiosome of dying with poison and can obtain two with same way and exempt from duck viral hepatitis antigen, and at room temperature preserve standby through inoculation duck viral hepatitis kind;
Producing of e, allos animal serum
Producing of allos animal bigeminy hyper-immune serum
The healthy anosis allos animal of choosing is (as horse, cattle, pig, sheep, rabbit etc.), by selected allos the weight of animals per 10 kilograms at its cervical region, abdominal part both sides multiple spot (5-10 point) subcutaneous injection one is exempted from duck pestilence antigen and one and is exempted from each 1 milliliter of duck viral hepatitis antigen, carrying out two after 7 days exempts from, allos animal after promptly exempting to one, inject 1 milliliter two at its subcutaneous multiple spot (5-10 point) and exempt from duck pestilence antigen and 1 milliliter two and exempt from duck viral hepatitis antigen for per 10 kilograms by its body weight, carrying out three again after 7 days exempts from, allos animal after promptly exempting to two, by its body weight per 10 kilograms at neck, 3.5 milliliter one of abdominal part multiple spot (5-10 point) injection exempted from duck pestilence antigen and 3.5 milliliter one and exempted from duck viral hepatitis antigen.Three exempt from the injection back every blood sampling in 5 days once, carrying out antibody titer detects, after 30 days when antibody titer 1: 16-1: during 128 scopes, this allos animal is taken a blood sample under aseptic condition, the blood that to be gathered is put into sterile chamber and is carried out drawing its serum after the natural separation, after in its serum, adding 2000 unit penicillins and 2000 unit streptomycins by every milliliter of serum, promptly become the bigeminy hyper-immune serum behind the 1/10000 amount adding thimerosal by its gross weight again, to its hyper-immune serum packing, seal, and place under-18 ℃ of conditions and preserve.
2, the preparation method of anti-duck pestilence according to claim 1 and duck viral hepatitis allos animal hyper-immune serum, it is characterized in that single injection duck pestilence antigen or duck viral hepatitis antigen on the anosis allos animal of health, can produce the hyper-immune serum of simple function respectively, promptly anti-duck pestilence allos animal hyper-immune serum or anti-duck viral hepatitis allos animal hyper-immune serum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95112185A CN1072508C (en) | 1995-10-30 | 1995-10-30 | Preparation of heterogenic animal high immune serum against duck pest and viral hepatitis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95112185A CN1072508C (en) | 1995-10-30 | 1995-10-30 | Preparation of heterogenic animal high immune serum against duck pest and viral hepatitis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1127139A true CN1127139A (en) | 1996-07-24 |
| CN1072508C CN1072508C (en) | 2001-10-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95112185A Expired - Fee Related CN1072508C (en) | 1995-10-30 | 1995-10-30 | Preparation of heterogenic animal high immune serum against duck pest and viral hepatitis |
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| CN (1) | CN1072508C (en) |
Cited By (9)
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| CN101961488A (en) * | 2010-10-20 | 2011-02-02 | 郑州后羿制药有限公司 | Method for preparing serum for resisting Muscovy duck parvovrius |
| CN102228482A (en) * | 2011-06-28 | 2011-11-02 | 重庆市畜牧科学院 | Propolis injection for preventing duck hemorrhagic oophoritis and preparation method thereof |
| CN102228481A (en) * | 2011-06-28 | 2011-11-02 | 重庆市畜牧科学院 | Propolis injection for preventing type I duck virus hepatitis and preparation method thereof |
| CN102258539A (en) * | 2011-06-28 | 2011-11-30 | 重庆市畜牧科学院 | Bee glue injection for preventing duck plague and preparation method thereof |
| CN102716485A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Dual hyper-immune egg yolk antibody injection for duck virus hepatitis and duck plague and preparation method thereof |
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| CN103018436A (en) * | 2012-12-14 | 2013-04-03 | 山东滨州沃华生物工程有限公司 | Method for preparing avian infectious bronchitis positive serum from domestic rabbit |
| CN104926939A (en) * | 2014-11-28 | 2015-09-23 | 四川农业大学 | Preparation methods for duck hepatitis virus immunogen and hyperimmune serum and application of duck hepatitis virus hyperimmune serum |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4762714A (en) * | 1986-04-08 | 1988-08-09 | Miles Laboratories, Inc. | Preparation of retrovirus-free immunoglobulins |
| GB9105899D0 (en) * | 1991-03-20 | 1991-05-08 | Wellcome Found | Therapeutic nucleosides |
| RU2035918C1 (en) * | 1991-10-23 | 1995-05-27 | Всероссийский государственный научно-исследовательский институт контроля, стандартизации и сертификации ветеринарных препаратов | Method for producing duck virus hepatitis vaccine |
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1995
- 1995-10-30 CN CN95112185A patent/CN1072508C/en not_active Expired - Fee Related
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| CN101961488A (en) * | 2010-10-20 | 2011-02-02 | 郑州后羿制药有限公司 | Method for preparing serum for resisting Muscovy duck parvovrius |
| CN101961488B (en) * | 2010-10-20 | 2013-09-11 | 郑州后羿制药有限公司 | Method for preparing serum for resisting Muscovy duck parvovrius |
| CN102228482A (en) * | 2011-06-28 | 2011-11-02 | 重庆市畜牧科学院 | Propolis injection for preventing duck hemorrhagic oophoritis and preparation method thereof |
| CN102228481A (en) * | 2011-06-28 | 2011-11-02 | 重庆市畜牧科学院 | Propolis injection for preventing type I duck virus hepatitis and preparation method thereof |
| CN102258539A (en) * | 2011-06-28 | 2011-11-30 | 重庆市畜牧科学院 | Bee glue injection for preventing duck plague and preparation method thereof |
| CN102228481B (en) * | 2011-06-28 | 2013-03-13 | 重庆市畜牧科学院 | Propolis injection for preventing type I duck virus hepatitis and preparation method thereof |
| CN102716485A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Dual hyper-immune egg yolk antibody injection for duck virus hepatitis and duck plague and preparation method thereof |
| CN102716483A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Duck plague yolk antibody freeze-dried powder and preparation method thereof |
| CN102772799A (en) * | 2012-05-31 | 2012-11-14 | 郑州后羿制药有限公司 | Duplex egg yolk antibody freeze-drying powder for duck virus hepatitis and duck plague and preparation method thereof |
| CN103018436A (en) * | 2012-12-14 | 2013-04-03 | 山东滨州沃华生物工程有限公司 | Method for preparing avian infectious bronchitis positive serum from domestic rabbit |
| CN104926939A (en) * | 2014-11-28 | 2015-09-23 | 四川农业大学 | Preparation methods for duck hepatitis virus immunogen and hyperimmune serum and application of duck hepatitis virus hyperimmune serum |
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