CN102228482A - Propolis injection for preventing duck hemorrhagic oophoritis and preparation method thereof - Google Patents
Propolis injection for preventing duck hemorrhagic oophoritis and preparation method thereof Download PDFInfo
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- CN102228482A CN102228482A CN2011101771313A CN201110177131A CN102228482A CN 102228482 A CN102228482 A CN 102228482A CN 2011101771313 A CN2011101771313 A CN 2011101771313A CN 201110177131 A CN201110177131 A CN 201110177131A CN 102228482 A CN102228482 A CN 102228482A
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Abstract
The invention relates to the field of animal prevention medicine, particularly to a preparation method for an injection for preventing animal epidemic diseases from occurring, wherein the propolis injection for preventing duck hemorrhagic oophoritis is composed of a propolis aqueous solution, a duck embryo homogenated tissue inoculated with duck flavivirus-Tembusu virus and dead by infection, and an inactivation solution; the preparation method comprises the step of adding the inactivation solution in the mixed solution of the duck embryo homogenated tissue and propolis to obtain the propolis injection for preventing duck hemorrhagic oophoritis; the propolis injection for preventing duck hemorrhagic oophoritis prepared by the preparation method of the invention has a remarkable effect to prevent duck hemorrhagic oophoritis (duck Tembusu virus disease); the protection ratio is increased to 90-100%, the protection period is prolonged to 6-12 months, the action time is shortened to 5 days, and only once injection can achieve an expected effect.
Description
Technical field
The present invention relates to a kind of zooprophylazis field of medicaments, be specially a kind of preparation method of preventing the injection of animal epidemic generation.
Background technology
Duck hemorrhagic oophoritis (duckling virosis) is that (Tembusu virus TMUV), causes the acute infectious disease of kind of duck and laying ducks by the Flavivirus tembusu virus, with the burst characteristics that sharply drop to of laying eggs, because of this disease mainly causes follicle degeneration, distortion, theca folliculi hyperemia, hemorrhage is called this disease duck hemorrhagic oophoritis temporarily, involve a wide range of knowledge, sickness rate height, mortality rate 5%-30%, sick duck heating, spirit is not good enough, the grass green dysentery, astasia, both legs paralysis, backward or the side stretch; Pathological changes is mainly seen in ovary, shows as ovarian dysgenesis, follicle degeneration, distortion, and theca folliculi hyperemia, hemorrhage, uterus and fallopian tube mucosa no abnormality seen, [Cao Zhenzhen opens and deposits Huang Yu etc. the visible yolk peritonitis report of some cases.The preliminary study of duck hemorrhagic oophoritis.China veterinary magazine (the 46th volume) the 12nd phase 3-7 in 2010].Bring great economic loss for the unit or the individual that raise duck fowl, simultaneously,, often cause stretching of virus, produce bigger disastrous effect owing to the duck fowl that disease is died is dealt with improperly.At present, do not find a kind of active drug that efficiently is exclusively used in prevention duck tembusu virus eqpidemic disease in the society as yet, this is that duck poultry raising unit or individual feel pain in the neck most.
The purpose of this invention is to provide a kind of safety good, have no side effect, protective rate height, protection period are long, produce short, easy to use anti-duck tembusu virus injection and preparation method of action time.
Summary of the invention
One of purpose of the present invention is to provide a kind of prevention duck hemorrhagic oophoritis (duckling Tobamovirus-tembusu virus (Tembusu virus, TMUV) disease, hereinafter to be referred as " duck hemorrhagic oophoritis or duck tembusu virus disease ") the propolis injection, this injection has preventive effect preferably to duck hemorrhagic oophoritis.
For achieving the above object, technical scheme of the present invention is:
The propolis injection of described prevention duck hemorrhagic oophoritis is made up of propolis solution, dead duck embryo homogenate tissue and the deactivation liquid of inoculation duckling Tobamovirus-tembusu virus and infection back, the weight ratio of described propolis solution, duck embryo homogenate tissue is 8-9:1-2, and described propolis solution is that volume fraction is 0.1-2%; Described inactivator is penicillin, streptomycin and formalin solution, every milliliter contains the penicillin of 2000 units and the streptomycin of 2000 units in the propolis injection of prevention duck hemorrhagic oophoritis, and described formalin volume fraction in the propolis injection of prevention duck hemorrhagic oophoritis is 0.4%.
Two of purpose of the present invention is to provide a kind of preparation method of preventing the propolis injection of duck hemorrhagic oophoritis, and this method is simple to operate, is applicable to large-scale production.
For achieving the above object, technical scheme of the present invention is:
The preparation method of the propolis injection of described prevention duck hemorrhagic oophoritis, specifically may further comprise the steps: a will inoculate duckling Tobamovirus-tembusu virus (Tembusu virus, TMUV) plant the malicious dead back duck embryo that also infects and make duck embryo homogenate tissue, with propolis and aqueous medium mix homogeneously, get the homogenate of duck embryo and organize the propolis mixed liquor, it is the propolis solution of 0.1-2% that the homogenate of described duck embryo organizes in the propolis mixed liquor propolis and aqueous medium to form volumetric concentration, and the volume ratio of described propolis solution and duck embryo homogenate tissue is 8-9:1-2;
B organizes in the homogenate of step a gained duck embryo and adds deactivation liquid in the propolis mixed liquor, make in the propolis injection of described prevention duck hemorrhagic oophoritis every milliliter to contain 2000 unit penicillins and 2000 unit streptomycins, and volume fraction is 0.4% formalin solution, 37-39 ℃ of deactivation must prevent the propolis injection of duck hemorrhagic oophoritis.
Further, the preparation method of the propolis injection of described prevention duck hemorrhagic oophoritis, described duckling Tobamovirus-tembusu virus (Tembusu virus, TMUV) plant poison and be specially:
A gets under aseptic condition because of suffering from the duck internal organs of duck hemorrhagic oophoritis death, puts into tissue mashing machine and smashs to pieces;
B gets and accounts for total amount be the duck tissue smashed to pieces of the quilt of 10-20% with accounting for gross weight is that the concentration of 80-90% is that the normal saline of 0.8 % mixes, and forms line and staff control's suspension;
C puts into centrifuge with line and staff control's suspension, gets its supernatant after centrifugal 20-40 minute;
In the clear liquid, the proportioning that adds 2000 unit penicillins and 2000 unit streptomycins by every milliliter of supernatant adds penicillin and streptomycin to d thereon, duckling virosis kind poison.
Further, the preparation method of the propolis injection of described prevention duck hemorrhagic oophoritis, dead duck internal organs described in the step a be in ovary, the heart, liver, spleen, lung and the brain any one or more.
Further, the preparation method of the propolis injection of described prevention duck hemorrhagic oophoritis, (Tembusu virus, TMUV) plant the dosage that poison is inoculated in the duck embryo allantois of 9-12 age in days is 0.1mL to described duckling Tobamovirus-tembusu virus.
Further, described prevention duck hemorrhagic oophoritis propolis injection contains 2000 unit penicillins and 2000 unit streptomycins for every milliliter, and adds formalin solution by 0.4% proportioning of total amount, 37-39 ℃ of deactivation 24 hours.
The propolis injection of the prevention duck hemorrhagic oophoritis made from preparation method of the present invention has remarkable result for prevention duck hemorrhagic oophoritis (duck tembusu virus disease); protective rate is brought up to 90-100%; protection period extends to 6-12 month; produce and shorten to 5 days action time, only need a shot to produce a desired effect.
The specific embodiment
Embodiment 1
Under aseptic condition, get and put into tissue mashing machine because of the duck internal organs (comprising the heart, liver, spleen, lung, brain and ovary) of suffering from the death of duck hemorrhagic oophoritis and smash to pieces, get the duck tissue that 10% the quilt that accounts for gross weight is smashed to pieces, 90% concentration that accounts for gross weight is that the normal saline of 0.8 % mixes, form line and staff control's suspension, and put it in the centrifuge under 4 000 rev/mins of conditions centrifugal 20 minutes, get its supernatant, promptly form duck hemorrhagic oophoritis kind poison after in supernatant, adding 2 000 unit penicillins and 2 000 unit streptomycins by every milliliter of supernatant.With duck hemorrhagic oophoritis kind poison put into concentration be 0.8% normal saline dilution l00 doubly, form duckling Tobamovirus-tembusu virus (Tembusu virus, TMUV) plant malicious diluent, duck Tan Busu kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 9 ages in days, be chosen at after the inoculation 48 hours hemorrhage, the duck embryo of feature death such as edema, putting into tissue mashing machine smashs to pieces, get duck homogenate tissue, the duck homogenate tissue of getting 10 parts in weight portion mixes with 90 parts propolis solution, the propolis final concentration reaches 0.1%, form line and staff control's suspension, and put it in the centrifuge, after under 4000 rev/mins of conditions centrifugal 20 minutes, get its supernatant, be that the propolis mixed liquor is organized in the homogenate of duck embryo, organize adding deactivation liquid in the propolis mixed liquor in the homogenate of gained duck embryo, make in the propolis injection of prevention duck hemorrhagic oophoritis every milliliter to contain 2000 unit penicillins and 2000 unit streptomycins, and volume fraction is 0.4% formalin solution, 37-39 ℃ of deactivation must prevent the propolis injection of duck hemorrhagic oophoritis (duck tembusu virus disease).
Embodiment 2
Under aseptic condition, get and put into tissue mashing machine because of the duck internal organs (comprising the heart, liver, spleen, lung, brain and ovary) of suffering from the death of duck hemorrhagic oophoritis and smash to pieces, get the duck tissue that 15% the quilt that accounts for gross weight is smashed to pieces, 85% concentration that accounts for gross weight is 0.8% normal saline mixing, form line and staff control's suspension, and put it in the centrifuge under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, promptly form duck viral oophoritis kind poison after in supernatant, adding 2 000 unit penicillins and 2 000 unit streptomycins by every milliliter of supernatant.Duck viral oophoritis kind poison is put into 100 times of the normal saline dilutions that concentration is 0.8 %, form duck hemorrhagic oophoritis kind poison diluent, duck hemorrhagic oophoritis diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 11 ages in days, be chosen at after the inoculation 80 hours hemorrhage, the duck embryo of feature death such as edema is put into tissue mashing machine and is smashed to pieces, get duck homogenate tissue, the duck homogenate tissue of getting 15 parts in weight portion mixes with 85 parts propolis solution, the propolis final concentration reaches 2%, form line and staff control's suspension, and put it in the centrifuge, after under 4 000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, be that the propolis mixed liquor is organized in the homogenate of duck embryo, organize adding deactivation liquid in the propolis mixed liquor in the homogenate of gained duck embryo, make in the propolis injection of prevention duck hemorrhagic oophoritis every milliliter to contain 2000 unit penicillins and 2000 unit streptomycins, and volume fraction is 0.4% formalin solution, 37-39 ℃ of deactivation must prevent the propolis injection of duck hemorrhagic oophoritis (duck tembusu virus disease).
Embodiment 3
Under aseptic condition, get and put into tissue mashing machine because of the duck internal organs (comprising the heart, liver, spleen, lung, brain and ovary) of suffering from the death of duck hemorrhagic oophoritis and smash to pieces, get the duck tissue that 20% the quilt that accounts for gross weight is smashed to pieces, 80% concentration that accounts for gross weight is 0.8% normal saline mixing, form line and staff control's suspension, and put it in the centrifuge under 4 000 rev/mins of conditions centrifugal 20 minutes, get its supernatant, promptly form duckling virosis kind poison after in supernatant, adding 2000 unit penicillins and 2000 unit streptomycins by every milliliter of supernatant.It is 100 times of 0.8% normal saline dilutions that duckling virosis kind poison is put into concentration, form duckling virosis kind poison diluent, duckling virosis kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 12 ages in days, be chosen at after the inoculation 48 hours hemorrhage, the duck embryo of feature death such as edema is put into tissue mashing machine and is smashed to pieces, get duck homogenate tissue, the duck homogenate tissue of getting 20 parts in weight portion mixes with 80 parts of propolis solutions, the propolis final concentration reaches 1%, form line and staff control's suspension, and it puts into centrifuge, after under 4 000 rev/mins of conditions centrifugal 40 minutes, get its supernatant, be that the propolis mixed liquor is organized in the homogenate of duck embryo, organize in the propolis mixed liquor in the homogenate of gained duck embryo to add deactivation liquid, make in the propolis injection of prevention duck hemorrhagic oophoritis every milliliter to contain 2000 unit penicillins and 2000 unit streptomycins, and volume fraction is 0.4% formalin solution, 37-39 ℃ of deactivation must prevent the propolis injection of duck hemorrhagic oophoritis (duck tembusu virus disease).
Embodiment 4
Under aseptic condition, get and put into tissue mashing machine because of the duck internal organs (comprising the heart, liver, spleen, lung, brain and ovary) of suffering from the death of duck hemorrhagic oophoritis and smash to pieces, get the duck tissue that 15% the quilt that accounts for gross weight is smashed to pieces, 85% concentration that accounts for gross weight is 0.8% normal saline mixing, form line and staff control's suspension, and put it in the centrifuge under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, promptly form duck hemorrhagic oophoritis kind poison after in supernatant, adding 2000 unit penicillins and 2 000 unit streptomycins by every milliliter of supernatant.It is 100 times of 0.8% normal saline dilutions that duck hemorrhagic oophoritis kind poison is put into concentration, form duck hemorrhagic oophoritis kind poison diluent, duck hemorrhagic oophoritis kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 11 ages in days, be chosen at after the inoculation 80 hours hemorrhage, the duck embryo of feature death such as edema is put into tissue mashing machine and is smashed to pieces, get duck homogenate tissue, the duck homogenate tissue of getting 15 parts in weight portion mixes with 85 parts of aqueous mediums, form line and staff control's suspension, and put it in the centrifuge, after under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, be that the propolis mixed liquor is organized in the homogenate of duck embryo, organize adding deactivation liquid in the propolis mixed liquor in the homogenate of gained duck embryo, make in the propolis injection of prevention duck hemorrhagic oophoritis every milliliter to contain 2000 unit penicillins and 2000 unit streptomycins, and volume fraction is 0.4% formalin solution, and 37-39 ℃ of deactivation must prevent the propolis injection of duck hemorrhagic oophoritis (duck tembusu virus disease).
Embodiment 5
Under aseptic condition, get and put into tissue mashing machine because of the duck internal organs (comprising the heart, liver, spleen, lung, brain, ovary) of suffering from the death of duck hemorrhagic oophoritis and smash to pieces, get the duck tissue that 15% the quilt that accounts for gross weight is smashed to pieces, 85% concentration that accounts for gross weight is 0.8% normal saline mixing, form line and staff control's suspension, and put it in the centrifuge under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, promptly form duckling virosis kind poison after in supernatant, adding 2 000 unit penicillins and 2 000 unit streptomycins by every milliliter of supernatant.Duckling virus is planted poison, and to put into concentration be 100 times of 0.8% normal saline dilutions, form duckling virosis kind poison diluent, duckling virosis kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 11 ages in days, be chosen at after the inoculation 80 hours hemorrhage, the duck embryo of feature death such as edema is put into tissue mashing machine and is smashed to pieces, get duck homogenate tissue, the duck homogenate tissue of getting 15 parts in weight portion mixes with 85 parts of propolis solutions, the propolis final concentration reaches 3%, form line and staff control's suspension, and put it in the centrifuge, after under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, be that the propolis mixed liquor is organized in the homogenate of duck embryo, organize in the propolis mixed liquor in the homogenate of gained duck embryo to add deactivation liquid, make in the propolis injection of prevention duck hemorrhagic oophoritis every milliliter to contain 2000 unit penicillins and 2000 unit streptomycins, and volume fraction is 0.4% formalin solution, 37-39 ℃ of deactivation must prevent the propolis injection of duck hemorrhagic oophoritis (duck tembusu virus disease).
Embodiment 6
Under aseptic condition, get and put into tissue mashing machine because of the duck internal organs (comprising the heart, liver, spleen, lung, brain, ovary) of suffering from the death of duck hemorrhagic oophoritis and smash to pieces, get the duck tissue that 15% the quilt that accounts for gross weight is smashed to pieces, 85% concentration that accounts for gross weight is 0.8% normal saline mixing, form line and staff control's suspension, and put it in the centrifuge under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, promptly form duckling virosis kind poison after in supernatant, adding 2000 unit penicillins and 2 000 unit streptomycins by every milliliter of supernatant.Duckling virus is planted poison, and to put into concentration be 100 times of 0.8% normal saline dilutions, form duckling virosis kind poison diluent, duckling virosis kind poison diluent with 0.1 milliliter of dosage is inoculated in the duck embryo allantois of 11 ages in days, be chosen at after the inoculation 80 hours hemorrhage, the duck embryo of feature death such as edema is put into tissue mashing machine and is smashed to pieces, get duck homogenate tissue, the duck homogenate tissue of getting 15 parts in weight portion mixes with 85 parts of white oil emulsions, white oil emulsion final concentration reaches 2%, form line and staff control's suspension, and put it in the centrifuge, after under 4000 rev/mins of conditions centrifugal 30 minutes, get its supernatant, be that the propolis mixed liquor is organized in the homogenate of duck embryo, organize in the propolis mixed liquor in the homogenate of gained duck embryo to add deactivation liquid, make in the propolis injection of prevention duck hemorrhagic oophoritis every milliliter to contain 2000 unit penicillins and 2000 unit streptomycins, and volume fraction is 0.4% formalin solution, 37-39 ℃ of deactivation must prevent the propolis injection of duck hemorrhagic oophoritis (duck tembusu virus disease).
The detection of embodiment 7 duck hemorrhagic oophoritis
Get homogenate tissue among the embodiment 1-6 and extract test kit with TRIzol Reagent RNA and carry out the extraction of viral RNA, the method for using RT-PCR carry out the detection of duck virus [Wan Chun and, Shi Shaohua, Cheng Longfei, Chen Hongmei, Fu Guanghua, Peng Chunxiang, Lin Fang, Lin Jiansheng, Huang Yu.The foundation of duck hemorrhagic oophoritis virus RT-PCR detection method.Fujian agriculture journal 2011,1:10-12], testing result is all positive.
Embodiment 8 aseptic detections
The injection of getting embodiment 1-6 preparation is inoculated in broth medium and blood agar plate respectively, through 37 ℃ of cultivations 24 hours, observes according to a conventional method, and assorted bacterium detects negative.
Embodiment 9 safety tests
Select for use in the meat-type duck of non-immune 11 ages in days, 10 every group, to press the injection of embodiment 1-6 preparation and divide, 6 groups and the blank group of normal saline amount to 7 groups; Every injection 1mL, isolated rearing 15 days, do every day body temperature, breathing, spirit, appetite, feces, etc. clinic observation, after injecting above-mentioned vaccine, the inoculation group does not all cause the vaccine morbidity with dead, and tangible pathological change is not seen in the injection site, have no adverse reaction, spirit, appetite is good.
Embodiment 10 antibody tests
Select for use in the meat-type duck of non-immune 11 ages in days, 10 every group, to press the injection of embodiment 1-6 preparation and divide, 6 groups and the blank group of normal saline amount to 7 groups;
Detection method: indirect ELISA detection method.
The result sees table 1 for details.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (5)
1. prevent the propolis injection of duck hemorrhagic oophoritis, it is characterized in that, the propolis injection of described prevention duck hemorrhagic oophoritis is made up of propolis solution, dead duck embryo homogenate tissue and the deactivation liquid of inoculation duckling Tobamovirus-tembusu virus and infection back, the weight ratio of described propolis solution, duck embryo homogenate tissue is 8-9:1-2, and described propolis solution is that volume fraction is 0.1-2%; Described inactivator is penicillin, streptomycin and formalin solution, every milliliter contains the penicillin of 2000 units and the streptomycin of 2000 units in the propolis injection of prevention duck hemorrhagic oophoritis, and described formalin volume fraction in the propolis injection of prevention duck hemorrhagic oophoritis is 0.4%.
2. the preparation method of the propolis injection of the described prevention of claim 1 duck hemorrhagic oophoritis is characterized in that, specifically may further comprise the steps:
A will inoculate duckling Tobamovirus-tembusu virus kind poison and infect dead back duck embryo and make duck embryo homogenate tissue, with propolis and aqueous medium mix homogeneously, get the homogenate of duck embryo and organize the propolis mixed liquor, it is the propolis solution of 0.1-2% that the homogenate of described duck embryo organizes in the propolis mixed liquor propolis and aqueous medium to form volumetric concentration, and the volume ratio of described propolis solution and duck embryo homogenate tissue is 8-9:1-2;
bOrganize adding deactivation liquid in the propolis mixed liquor in the homogenate of step a gained duck embryo, make in the propolis injection of described prevention duck hemorrhagic oophoritis every milliliter to contain 2000 unit penicillins and 2000 unit streptomycins, and volume fraction is 0.4% formalin solution, 37-39 ℃ of deactivation must prevent the propolis injection of duck hemorrhagic oophoritis.
3. the preparation method of the propolis injection of prevention duck hemorrhagic oophoritis according to claim 2 is characterized in that, the sick poison of planting of described duckling virus-tembusu virus is specially:
A gets under aseptic condition because of suffering from the duck internal organs of duck hemorrhagic oophoritis death, puts into tissue mashing machine and smashs to pieces;
B gets and accounts for total amount be the duck tissue smashed to pieces of the quilt of 10-20% with accounting for gross weight is that the concentration of 80-90% is that the normal saline of 0.8 % mixes, and forms line and staff control's suspension;
C puts into centrifuge with line and staff control's suspension, gets its supernatant after centrifugal 20-40 minute;
In the clear liquid, the proportioning that adds 2000 unit penicillins and 2000 unit streptomycins by every milliliter of supernatant adds penicillin and streptomycin to d thereon, the sick poison of planting of duckling virus-tembusu virus.
4. the preparation method of the propolis injection of prevention according to claim 2 duck hemorrhagic oophoritis is characterized in that, dead duck internal organs described in the step a be in the heart, liver, spleen, lung, ovary and the brain any one or more.
5. the preparation method of the propolis injection of prevention according to claim 3 duck hemorrhagic oophoritis is characterized in that: the dosage that described duckling virus-tembusu virus kind poison is inoculated in the duck embryo allantois of 9-12 age in days is 0.1mL.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102488893A (en) * | 2011-12-28 | 2012-06-13 | 瑞普（保定）生物药业有限公司 | Duck hemorrhagic oophoritis inactivated vaccine production method by using cell line and product thereof |
| CN102977194A (en) * | 2012-11-22 | 2013-03-20 | 青岛宝依特生物制药有限公司 | Duck tembusu virus (DTMUV) E protein gene and application thereof |
| CN109295010A (en) * | 2018-10-10 | 2019-02-01 | 扬州大学 | A method of tembusu virus efficient amplification is carried out using chicken liver cell |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127139A (en) * | 1995-10-30 | 1996-07-24 | 鹿忠孝 | Preparation of heterogenic animal high immune serum against duck pest and viral hepatitis |
| CN101475639A (en) * | 2008-08-19 | 2009-07-08 | 福州大北农生物技术有限公司 | Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof |
-
2011
- 2011-06-28 CN CN2011101771313A patent/CN102228482B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127139A (en) * | 1995-10-30 | 1996-07-24 | 鹿忠孝 | Preparation of heterogenic animal high immune serum against duck pest and viral hepatitis |
| CN101475639A (en) * | 2008-08-19 | 2009-07-08 | 福州大北农生物技术有限公司 | Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《中国家禽》 20061231 杨永刚等 鸭传染性浆膜炎蜂胶灭活苗的研制及免疫效果试验 第28卷, 第9期 * |
| 《畜牧兽医科技信息》 20041231 孙刚等 鸭大肠杆菌多价蜂胶灭活苗 , 第2期 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102488893A (en) * | 2011-12-28 | 2012-06-13 | 瑞普（保定）生物药业有限公司 | Duck hemorrhagic oophoritis inactivated vaccine production method by using cell line and product thereof |
| CN102488893B (en) * | 2011-12-28 | 2013-07-17 | 瑞普（保定）生物药业有限公司 | Duck hemorrhagic oophoritis inactivated vaccine production method by using cell line and product thereof |
| CN102977194A (en) * | 2012-11-22 | 2013-03-20 | 青岛宝依特生物制药有限公司 | Duck tembusu virus (DTMUV) E protein gene and application thereof |
| CN109295010A (en) * | 2018-10-10 | 2019-02-01 | 扬州大学 | A method of tembusu virus efficient amplification is carried out using chicken liver cell |
| CN109295010B (en) * | 2018-10-10 | 2021-04-02 | 扬州大学 | Method for efficiently amplifying tembusu virus by using chicken hepatocytes |
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| CN102228482B (en) | 2012-12-12 |
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