CN112694975A - Kit for predicting lung injury clinical progress after breast tumor radiotherapy - Google Patents
Kit for predicting lung injury clinical progress after breast tumor radiotherapy Download PDFInfo
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- CN112694975A CN112694975A CN202110135377.8A CN202110135377A CN112694975A CN 112694975 A CN112694975 A CN 112694975A CN 202110135377 A CN202110135377 A CN 202110135377A CN 112694975 A CN112694975 A CN 112694975A
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention discloses a kit for predicting the clinical progress of lung injury after breast tumor radiotherapy, which comprises a kit body, wherein a simulation part for simulating lung tumor tissues, a reaction part for receiving radioactive rays and performing cell extinction, an acquisition part for acquiring the lung tissues, cells and blood after reaction and performing reagent reaction on the lung tissues, cells and blood, and an inspection part for forming an inspection result according to a reagent reaction result are arranged in the kit body; the simulation part collects tumor cells, normal lung tissues and blood of a patient in the body of the patient, and forms a three-dimensional culture environment simulating the in-vivo environment of a part of the patient participating in radiation in a biological culture medium; the reaction part is a part which is placed under the radioactive rays and receives the radiation therapy of the biological culture medium; the collecting part is a part for carrying out reagent experiments on the simulation part after receiving the radiotherapy; the testing part is a part which is formed by the simulation part and presents the test result after the collection part carries out reagent reaction; the invention is an analog prediction, and has higher precision and high inspection efficiency.
Description
Technical Field
The invention relates to the field of tumor treatment, in particular to a kit for predicting the clinical progress of lung injury after breast tumor radiotherapy.
Background
Tumor radiotherapy is a method for treating malignant tumors by using radiation, such as alpha, beta and gamma rays generated by radioactive isotopes and x rays, electron beams, proton beams and the like generated by various x-ray therapeutic machines or accelerators, so far, radiotherapy is still one of the important means for local treatment of malignant tumors, about 70 percent of cancer patients need radiotherapy in the process of treating cancer, and about 40 percent of cancer patients can achieve radical treatment through radiotherapy. The existing radiation therapy means mainly comprise conformal radiation therapy, intensity modulated radiation therapy, afterloading radiation therapy, particle implantation and the like, and different means can be selected according to different tumors and treatment purposes.
The radiotherapy of tumor itself has toxic and side effect to cancer patients, because the radiation has the same effect to cancer cells, normal tissues and organs, and has no selectivity, so doctors can select operation, radiotherapy, chemotherapy and comprehensive treatment according to the disease condition, so as to achieve the effect of radical treatment of cancer and preservation of body functions. Therefore, radiotherapy is generally combined with surgery, chemotherapy and traditional Chinese medicine comprehensive treatment, and the cure rate is high. In general, radiotherapy is directed at surface cancer cells, cannot achieve the effect of radical treatment, but has better inhibiting and cooperating treatment effects. Because of this feature, it is necessary to perform predictive analysis of the effect that can be achieved by radiation therapy before radiation therapy, and to judge the clinical treatment progress that can be achieved after radiation therapy. For patients with both lung injury and tumor, radiation therapy cannot be directly applied.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a kit for predicting the clinical progress of lung injury after breast tumor radiotherapy.
In order to achieve the purpose, the invention adopts the following technical scheme:
the kit for predicting the clinical progress of lung injury after breast tumor radiotherapy comprises a box body, wherein a simulation part for simulating lung tumor tissue, a reaction part for receiving radioactive rays and eliminating cells, an acquisition part for acquiring the lung tissue, the cells and the blood after reaction and performing reagent reaction on the lung tissue, the cells and the blood, and an inspection part for forming an inspection result according to a reagent reaction result are arranged in the box body; the simulation part collects tumor cells, normal lung tissues and blood of a patient in the body of the patient, and forms a three-dimensional culture environment simulating the in-vivo environment of a part of the patient participating in radiation in a biological culture medium; the reaction part is a part which is placed under the radioactive rays and receives the radiation therapy of the biological culture medium; the collecting part is a part for carrying out reagent experiments on the simulation part after receiving the radiotherapy; the test section is a section formed by the sample section and showing a test result after the sample section has reacted with the reagent.
Preferably, the cell objects to be examined in the collection portion include lymphocytes, dendritic cells, NK cells, macrophages, neutrophils, eosinophils, basophils, mast cells, erythrocytes, and platelets.
Preferably, the top of the box body is clamped with a box cover; the reagent box comprises a box body and a plurality of reagent cavities, wherein the box body comprises a bearing groove and the reagent cavities arranged below the bearing groove; and the bottom of the reagent cavity is provided with a thimble corresponding to the isolating membrane in a sliding way.
Preferably, the bottom of the box body is provided with a second through hole communicated with the reagent cavity, and the thimble is connected in the second through hole in a sliding manner; the bottom of the thimble is fixedly connected with a base, two rubber pads which are symmetrically arranged are clamped between the base and the bottom wall of the box body, and the rubber pads have viscosity; the initial position of the thimble is positioned below the isolating film; the rubber mat is in an elliptical ring shape, and one end of the rubber mat is fixedly connected with a ring-shaped pull ring.
Preferably, a third through hole is formed in the box cover, and two light-transmitting plates which are arranged in parallel are fixed in the third through hole; the inner wall of the box cover is provided with corrugated teeth matched with the box body in a clamping manner.
Preferably, the working method of the box body comprises the following steps: s1, completing the preparation of a biological culture medium in the bearing groove, and then completing the culture of the tumor cells and lung tissues sampled from the patient on the culture medium to form a simulation part; s2 performing radiotherapy on the simulation part by using radioactive rays through the transparent plate, and eliminating part of cells to death to form a reaction part; s3, inverting the box body, positioning the box cover at the bottom, taking out the rubber mat, puncturing the isolation membrane by the thimble, introducing the reagent in the reagent cavity into the containing groove, and reacting the cells with the reagent to form a collection part to be tested; s4, observing and sampling the cells in the bearing groove which completes the reaction, and determining the actual condition of the lung tissue after the reaction, thereby performing predictive analysis on the entity radiotherapy.
Preferably, the cell culture method comprises the following steps: s1 taking out the kit from-80 ℃, quickly inserting the kit into ice, after 5 minutes, adding the target DNA when the fungus block melts, slightly and uniformly mixing the target DNA with the bottom of an EP tube by hand, and standing the mixture in ice for 25 minutes; s2 is heat-shocked in 42 ℃ water bath for 45 seconds, quickly put back on ice and kept stand for 2 minutes; s3 adding 0.9 mL of one of a room temperature SOC culture medium and an LB culture medium into the centrifuge tube; s4 is revived for 60 minutes at 37 ℃ and 225rpm, or revived for 90 minutes at 30 ℃ and 225 rpm; s5 was centrifuged at 5000rpm for one minute to harvest the bacteria, 100. mu.l of the supernatant was left, and the resuspended pellet was gently pipetted and plated onto the corresponding antibiotic-containing medium.
The invention has the beneficial effects that: according to the invention, firstly, the box body of the kit is divided into four parts, the three-dimensional culture of cells is firstly realized, and the internal tissues of a human body are simulated, so that the simulation part is subjected to radiotherapy and then inspection analysis, the simulation type prediction of radiotherapy is realized, the prediction result is in line with the reality, and the accuracy is higher; the invention provides a kit structure, realizes the functions, integrates culture, reaction and inspection into a whole, and has high inspection efficiency.
Drawings
FIG. 1 is a schematic diagram of the operation of the kit according to the present invention;
FIG. 2 is a schematic structural diagram of the kit of the present invention.
Reference numbers in the figures: the reagent box comprises a box body 1, a containing groove 11, a reagent cavity 12, a first through hole 13, a second through hole 14, a box cover 2, a third through hole 21, a light-transmitting plate 3, an isolating membrane 4, a base 5, a thimble 51, a rubber ring 6 and a pull ring 61.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1 and 2, a kit for predicting clinical progress of lung injury after radiation therapy of breast tumor includes a kit body 1, a simulation part for simulating lung tumor tissue, a reaction part for receiving radiation and eliminating cells, a collection part for collecting lung tissue, cells and blood after reaction and performing reagent reaction on the lung tissue, cells and blood, and a test part for forming test result according to the reagent reaction result, wherein the kit body 1 is provided with a simulation part for simulating lung tumor tissue, a reaction part for receiving radiation and eliminating cells, a collection part for collecting lung tissue, cells and blood after reaction and; the simulation part collects tumor cells, normal lung tissues and blood of a patient in the body of the patient, and forms a three-dimensional culture environment simulating the in-vivo environment of a part of the patient participating in radiation in a biological culture medium; the reaction part is a part which is placed under the radioactive rays and receives the radiation therapy of the biological culture medium; the collecting part is a part for carrying out reagent experiments on the simulation part after receiving the radiotherapy; the test section is a section formed by the sample section and showing a test result after the sample section has reacted with the reagent.
In this example, the cell objects to be examined in the collection portion include lymphocytes, dendritic cells, NK cells, macrophages, neutrophils, eosinophils, basophils, mast cells, erythrocytes, and platelets.
In the embodiment, the top of the box body 1 is clamped with a box cover 2; the box body 1 comprises a bearing groove 11 and a reagent cavity 12 arranged below the bearing groove 11, a plurality of first through holes 13 are communicated between the bearing groove 11 and the reagent cavity 12, and an isolating membrane 4 for isolating the bearing groove 11 from the reagent cavity 12 is fixed in each first through hole 13; a thimble 51 corresponding to the isolation film 4 is slidably mounted on the bottom of the reagent chamber 12.
In this embodiment, the bottom of the box body 1 is provided with a second through hole 14 communicated with the reagent cavity 12, and the thimble 51 is slidably connected in the second through hole 14; the bottom of the thimble 51 is fixedly connected with a base 5, two rubber pads 6 which are symmetrically arranged are clamped between the base 5 and the bottom wall of the box body 1, and the rubber pads 6 have viscosity; the initial position of the thimble 51 is positioned below the isolation film 4; the rubber cushion 6 is in an elliptical ring shape, and one end of the rubber cushion 6 is fixedly connected with a ring-shaped pull ring 61.
In this embodiment, the box cover 2 is provided with a third through hole 21, and two light-transmitting plates 3 arranged in parallel are fixed in the third through hole 21; the inner wall of the box cover 2 is provided with corrugated teeth which are matched with the box body 1 in a clamping way.
In this embodiment, the working method of the cartridge 1 includes the following steps: s1, completing the preparation of a biological culture medium in the loading groove 11, and then completing the culture of tumor cells and lung tissues sampled from a patient on the culture medium to form a simulation part; s2, the radioactive ray is transmitted through the translucent plate 3 to perform radiotherapy on the simulation part, and part of the cells are eliminated to form a reaction part; s3, inverting the box body 1, positioning the box cover 2 at the bottom, taking out the rubber mat 6, pricking the isolating membrane 4 by the thimble 51, introducing the reagent in the reagent cavity 12 into the containing groove 11, and reacting the cells with the reagent to form a collection part to be tested; s4, observing and sampling the cells in the bearing groove 11 which completes the reaction, and determining the actual condition of the lung tissue after the reaction, thereby performing the predictive analysis on the entity radiotherapy.
In this embodiment, the cell culture method comprises the following steps: s1 taking out the kit from-80 ℃, quickly inserting the kit into ice, after 5 minutes, adding the target DNA when the fungus block melts, slightly and uniformly mixing the target DNA with the bottom of an EP tube by hand, and standing the mixture in ice for 25 minutes; s2 is heat-shocked in 42 ℃ water bath for 45 seconds, quickly put back on ice and kept stand for 2 minutes; s3 adding 0.9 mL of one of a room temperature SOC culture medium and an LB culture medium into the centrifuge tube; s4 is revived for 60 minutes at 37 ℃ and 225rpm, or revived for 90 minutes at 30 ℃ and 225 rpm; s5 was centrifuged at 5000rpm for one minute to harvest the bacteria, 100. mu.l of the supernatant was left, and the resuspended pellet was gently pipetted and plated onto the corresponding antibiotic-containing medium.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. The kit for predicting the clinical progress of lung injury after breast tumor radiotherapy is characterized by comprising a box body (1), wherein a simulation part for simulating lung tumor tissues, a reaction part for receiving radioactive rays and eliminating cells, an acquisition part for acquiring the lung tissues, the cells and the blood after reaction and performing reagent reaction on the lung tissues, the cells and the blood, and a test part for forming a test result according to the reagent reaction result are arranged in the box body (1); the simulation part collects tumor cells, normal lung tissues and blood of a patient in the body of the patient, and forms a three-dimensional culture environment simulating the in-vivo environment of a part of the patient participating in radiation in a biological culture medium; the reaction part is a part which is placed under the radioactive rays and receives the radiation therapy of the biological culture medium; the collecting part is a part for carrying out reagent experiments on the simulation part after receiving the radiotherapy; the test section is a section formed by the sample section and showing a test result after the sample section has reacted with the reagent.
2. The kit for predicting the clinical progression of lung injury after radiation therapy of breast tumors according to claim 1, wherein the cellular objects examined in the collection portion comprise lymphocytes, dendritic cells, NK cells, macrophages, neutrophils, eosinophils, basophils, mast cells, erythrocytes, platelets.
3. The kit for predicting the clinical progress of lung injury after radiation therapy of breast tumor as claimed in claim 1, wherein the top of the box body (1) is clamped with a box cover (2); the kit body (1) comprises a bearing groove (11) and a reagent cavity (12) arranged below the bearing groove (11), a plurality of first through holes (13) are communicated between the bearing groove (11) and the reagent cavity (12), and an isolating membrane (4) for isolating the bearing groove (11) from the reagent cavity (12) is fixed in each first through hole (13); and a thimble (51) corresponding to the isolating membrane (4) is slidably mounted at the bottom of the reagent cavity (12).
4. The kit for predicting the clinical progress of lung injury after radiation therapy of breast tumor as claimed in claim 3, wherein the bottom of the box body (1) is provided with a second through hole (14) communicated with the reagent cavity (12), the thimble (51) is slidably connected in the second through hole (14); the bottom of the thimble (51) is fixedly connected with a base (5), two rubber pads (6) which are symmetrically arranged are clamped between the base (5) and the bottom wall of the box body (1), and the rubber pads (6) have viscosity; the initial position of the thimble (51) is positioned below the isolating film (4); the rubber mat (6) is in an elliptical ring shape, and one end of the rubber mat (6) is fixedly connected with a ring-shaped pull ring (61).
5. The kit for predicting the clinical progress of lung injury after radiation therapy of breast tumor according to claim 4, wherein the box cover (2) is provided with a third through hole (21), and two light-transmitting plates (3) arranged in parallel are fixed in the third through hole (21); the inner wall of the box cover (2) is provided with corrugated teeth matched with the box body (1) in a clamping manner.
6. The kit for predicting the clinical progression of lung injury after radiation therapy of breast tumors according to claim 5, characterized in that the working method of said cartridge (1) comprises the following steps: s1, completing the preparation of a biological culture medium in the loading groove (11), and then completing the culture of tumor cells and lung tissues sampled from a patient on the culture medium to form a simulation part; s2, the simulation part is irradiated by the radioactive ray through the transparent plate (3) to eliminate the dead cells and form the reaction part; s3, inverting the box body (1), positioning the box cover (2) at the bottom, taking out the rubber mat (6), pricking the isolating membrane (4) by the thimble (51), introducing the reagent in the reagent cavity (12) into the containing groove (11), and reacting the cells with the reagent to form a collection part to be tested; s4, observing and sampling the cells in the bearing groove (11) which completes the reaction, and determining the actual condition of the lung tissue after the reaction, thereby performing the prediction analysis on the entity radiotherapy.
7. The kit for predicting the clinical progression of lung injury after radiation therapy of breast tumors according to claim 1, wherein the cell culture method comprises the following steps:
s1 taking out the kit from-80 ℃, quickly inserting the kit into ice, after 5 minutes, adding the target DNA when the fungus block melts, slightly and uniformly mixing the target DNA with the bottom of an EP tube by hand, and standing the mixture in ice for 25 minutes; s2 is heat-shocked in 42 ℃ water bath for 45 seconds, quickly put back on ice and kept stand for 2 minutes; s3 adding 0.9 mL of one of a room temperature SOC culture medium and an LB culture medium into the centrifuge tube; s4 is revived for 60 minutes at 37 ℃ and 225rpm, or revived for 90 minutes at 30 ℃ and 225 rpm; s5 was centrifuged at 5000rpm for one minute to harvest the bacteria, 100. mu.l of the supernatant was left, and the resuspended pellet was gently pipetted and plated onto the corresponding antibiotic-containing medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110135377.8A CN112694975A (en) | 2021-02-01 | 2021-02-01 | Kit for predicting lung injury clinical progress after breast tumor radiotherapy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110135377.8A CN112694975A (en) | 2021-02-01 | 2021-02-01 | Kit for predicting lung injury clinical progress after breast tumor radiotherapy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112694975A true CN112694975A (en) | 2021-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110135377.8A Withdrawn CN112694975A (en) | 2021-02-01 | 2021-02-01 | Kit for predicting lung injury clinical progress after breast tumor radiotherapy |
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| Country | Link |
|---|---|
| CN (1) | CN112694975A (en) |
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2021
- 2021-02-01 CN CN202110135377.8A patent/CN112694975A/en not_active Withdrawn
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Application publication date: 20210423 |