CN112679664A - Seaweed biological water-retaining agent and preparation method thereof - Google Patents
Seaweed biological water-retaining agent and preparation method thereof Download PDFInfo
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Abstract
The invention provides a seaweed biological water-retaining agent, which comprises the following raw materials in parts by weight: 30.0 to 60.0 portions of algal polysaccharide extract, 50.0 to 70.0 portions of sodium acrylate, 0.02 to 0.04 portion of N, N-methylene bisacrylamide, 0.2 to 0.3 portion of potassium persulfate and 5.0 to 10.0 portions of water. The seaweed polysaccharide extract and the monomer are polymerized to form the seaweed biological water-retaining agent, the product is safe and pollution-free, can absorb water with the weight of 300-fold and 1100-fold, can achieve the water-saving effect of agriculture and forestry, has the characteristic of biodegradability, and solves the technical problems of water or nutrient loss of soil-crops and the like.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a seaweed biological water-retaining agent and a preparation method thereof.
Background
Unreasonable reclamation and grazing in human production activities have great influence on soil, and natural vegetation of original land is seriously damaged, so that the desertification of the soil tends to be serious. Research shows that the soil added with the agricultural and forestry water-retaining agent in a certain proportion can improve the granular structure of the soil, improve the water retention and air permeability of the soil, reduce the day and night temperature difference change of the soil, adjust the dryness and humidity of the soil, reduce the irrigation times and achieve the aims of improving poor soil and resisting drought and harvest.
Since the 80 th century, developed countries such as the United states, Japan, England, France, Germany, etc. invested a lot of funds to develop and research the high water-absorbing water-retaining agent, and then successively invested in industrial production. The research on the super absorbent water retention agent in China starts from the beginning of the 80 th 20 th century, and dozens of super absorbent resin production enterprises in China at present. In recent years, the super absorbent water retention agent at home and abroad has various types, is rapidly developed and is widely applied, such as polyacrylic resin, polyacrylate-vinyl alcohol resin and the like. The resin has water absorption and retention performance and soil improvement capacity, but has the defects of high cost, insufficient water absorption and retention capacity, difficult biodegradation and the like.
Disclosure of Invention
In view of the above, the technical problem to be solved by the present invention is to provide a seaweed biological water retaining agent, which has good water absorption and retention functions, low cost and degradability.
The invention provides a seaweed biological water-retaining agent, which comprises the following raw materials in parts by weight:
30.0 to 60.0 portions of algal polysaccharide extract, 50.0 to 70.0 portions of sodium acrylate, 0.02 to 0.04 portion of N, N-methylene bisacrylamide, 0.2 to 0.3 portion of potassium persulfate and 5.0 to 10.0 portions of water.
Preferably, the sodium acrylate is obtained by partially neutralizing acrylic acid with sodium carbonate, and the neutralization degree is 65-75%.
Preferably, the preparation method of the algal polysaccharide extract comprises the following steps:
a) cleaning and crushing seaweed to obtain a seaweed substrate;
b) mixing the sodium citrate-protease complex with a seaweed substrate, and carrying out enzymolysis to obtain an enzymolysis product;
c) mixing and extracting sodium carbonate and an enzymolysis product to obtain a primary extract;
d) mixing the primary extract with citric acid, boric acid, sodium acetate and sodium tripolyphosphate, and extracting to obtain seaweed extract;
e) mixing alginate lyase and fucoidin lyase with the seaweed extract, and carrying out enzymolysis to obtain a crude seaweed polysaccharide extract;
f) ultrafiltering the crude extract to obtain algal polysaccharide extract.
Preferably, the seaweed is one or more of brown algae, red algae and green algae; the seaweed is naturally wild or artificially cultured.
Preferably, the seaweed is one or more dry seaweed of brown seaweed; preferably, the seaweed is kelp.
Preferably, the enzymolysis temperature in the step b) is 55-65 ℃; the enzymolysis time is 2-3 h;
the extraction temperature in the step c) is 75-85 ℃; the extraction time is 2-4 h;
the extraction temperature in the step d) is 55-65 ℃; the extraction time is 1-2 h, and the temperature is cooled to 40-50 ℃ after extraction;
the enzymolysis temperature of the step e) is 40-50 ℃; the enzymolysis time is 1-3 h;
and e) specifically, transferring the crude algal polysaccharide extract obtained in the step e) into ultrafiltration equipment, carrying out concentration operation, and removing macromolecular impurity components in a system for later use.
Preferably, the mass ratio of the sodium citrate-protease complex to the seaweed substrate is (10-40): (1-20): (100-300).
The mass ratio of the alginate lyase to the fucoidin lyase to the seaweed extract is (10-30): (5-20): (100-1000).
Preferably, the mass ratio of the extracting solution to the citric acid, the boric acid, the sodium acetate and the sodium tripolyphosphate is (100-): (1-20): (1-30): (5-40): (1-20).
The invention provides a preparation method of the seaweed biological water-retaining agent in any one of the technical schemes, which comprises the following steps:
adding sodium acrylate with neutralization degree of 65-75%, N-methylene bisacrylamide, potassium persulfate and water into the seaweed polysaccharide extract obtained in the seaweed activating step, fully mixing, heating, stirring, drying and crushing to obtain the seaweed biological water-retaining agent.
Preferably, the heating temperature is 65-80 ℃, and the heating time is 2-4 h.
Compared with the prior art, the invention provides a seaweed biological water-retaining agent which comprises the following raw materials in parts by weight: 30.0 to 60.0 portions of algal polysaccharide extract, 50.0 to 70.0 portions of sodium acrylate, 0.02 to 0.04 portion of N, N-methylene bisacrylamide, 0.2 to 0.3 portion of potassium persulfate and 5.0 to 10.0 portions of water. The seaweed polysaccharide extract and the monomer are polymerized to form the seaweed biological water-retaining agent, the product is safe and pollution-free, can absorb water with the weight of 300-fold and 1100-fold, can achieve the water-saving effect of agriculture and forestry, has the characteristic of biodegradability, and solves the technical problems of water or nutrient loss of soil-crops and the like.
Drawings
FIG. 1 is a graph showing the change of water absorption capacity of the seaweed biological water retaining agent in example 4 of the present invention with time.
Detailed Description
The invention provides a seaweed biological water-retaining agent and a preparation method thereof, and a person skilled in the art can use the contents in the text for reference and properly improve the process parameters for realization. It is expressly intended that all such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the scope of the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The invention provides a seaweed biological water-retaining agent, which comprises the following raw materials in parts by weight:
30.0 to 60.0 portions of algal polysaccharide extract, 50.0 to 70.0 portions of sodium acrylate, 0.02 to 0.04 portion of N, N-methylene bisacrylamide, 0.2 to 0.3 portion of potassium persulfate and 5.0 to 10.0 portions of water.
In the present invention, the above-mentioned raw materials are used in parts by weight, and the parts by weight are equivalent to mass% when the weight is 100.
The seaweed biological water retention agent provided by the invention comprises 30.0-60.0 parts by weight of seaweed polysaccharide extract; preferably 34.0 to 57.0 parts by weight; more preferably 37.0 to 53.0 parts by weight; most preferably 40.0 to 50.0 parts by weight.
According to the invention, the preparation method of the algal polysaccharide extract comprises the following steps:
a) cleaning and crushing seaweed to obtain a seaweed substrate;
b) mixing the sodium citrate-protease complex with a seaweed substrate, and carrying out enzymolysis to obtain an enzymolysis product;
c) mixing and extracting sodium carbonate and an enzymolysis product to obtain a primary extract;
d) mixing the extracting solution with citric acid, boric acid, sodium acetate and sodium tripolyphosphate, and extracting to obtain seaweed extracting solution;
e) mixing alginate lyase and fucoidin lyase with the seaweed extract, and carrying out enzymolysis to obtain a crude seaweed polysaccharide extract;
f) ultrafiltering the crude extract to obtain algal polysaccharide extract.
The preparation method of the seaweed polysaccharide extract firstly cleans and crushes seaweed to obtain a seaweed substrate.
The seaweed is one or more of brown algae, red algae and green algae; the seaweed is naturally wild or artificially cultured. Preferably, the seaweed is one or more dry seaweed of brown seaweed; more preferably, the seaweed is kelp.
The cleaning of the invention is to wash off the salt and impurities on the surface by using clean water. The present invention is not limited to the specific manner of cleaning, which is well known to those skilled in the art.
After cleaning, the raw materials are crushed, and the crushing is preferably to 60-100 meshes.
And mixing the sodium citrate-protease complex with the seaweed substrate, and carrying out enzymolysis to obtain an enzymolysis product.
The invention preferably adds the sodium citrate-protease complex to the seaweed substrate, extracts and enzymatically hydrolyzes.
Wherein the enzymolysis temperature is preferably 55-65 ℃; more preferably 55 to 63 ℃; the enzymolysis time is preferably 2-3 h; more preferably 2 to 2.8 hours.
The mass ratio of the sodium citrate-protease complex to the seaweed substrate is preferably (10-40): (1-20): (100-300).
Mixing and extracting sodium carbonate and an enzymolysis product to obtain a primary extract.
Sodium carbonate was added to the seaweed substrate and extraction was continued.
The mass ratio of the sodium carbonate to the enzymolysis product is preferably (1-20): (100-500).
The extraction temperature is preferably 75-85 ℃; more preferably 75 to 83 ℃; most preferably 75-81 ℃; the extraction time is preferably 2-4 h; more preferably 2.5-2.5 h; most preferably 3 hours.
Mixing the extract with citric acid, boric acid, sodium acetate and sodium tripolyphosphate, and extracting to obtain Sargassum extract.
Namely, the extracted solution is cooled to 55-60 ℃, the citric acid is added for extraction after heat preservation, the heating is stopped after the boric acid, the sodium acetate and the sodium tripolyphosphate are continuously added, and the natural cooling is carried out after the stirring is continuously carried out until the system is uniform.
Wherein the extraction temperature is preferably 55-65 ℃; the extraction time is preferably 1-2 h, and the temperature is cooled to 40-50 ℃ after extraction;
the mass ratio of the extracting solution to the citric acid, the boric acid, the sodium acetate and the sodium tripolyphosphate is preferably (100-) -400: (1-20): (1-30): (5-40): (1-20).
Mixing alginate lyase and fucoidin lyase with the seaweed extract, and carrying out enzymolysis to obtain the crude seaweed polysaccharide extract.
Transferring alginate lyase and fucoidin lyase into the seaweed extract, performing enzymolysis treatment, cooling to room temperature after enzymolysis, performing high-speed centrifugation, and filtering to obtain seaweed polysaccharide crude extract.
According to the invention, the enzymolysis temperature is preferably 40-50 ℃; more preferably 42 ℃ to 48 ℃; most preferably from 45 ℃ to 48 ℃. The enzymolysis time is preferably 1-3 h; more preferably 1.5-2.5 h; most preferably 2 h.
The mass ratio of the alginate lyase, the fucoidin lyase and the seaweed extract is preferably (10-30): (5-20): (100-1000).
The alginate lyase and the fucoidin lyase are provided for the thunder force balance agriculture development limited company.
Ultrafiltering the crude extract to obtain algal polysaccharide extract.
The ultrafiltration is specifically to transfer the crude algal polysaccharide extract into ultrafiltration equipment, carry out concentration operation, remove macromolecular impurity components in a system and reserve.
The invention prepares the algal polysaccharide extract by adopting the new technology of temperature difference wall breaking and double enzymolysis. The bioactive substances extracted by the technology have high content, and almost all the nutrients and functional components of the seaweed are reserved; in addition, the water retention agent has strong specificity and the characteristics of safety, greenness and environmental protection, wherein the content of alginic acid is 13.2 percent (37.6 percent of dry basis), so that the water retention property is better.
The seaweed biological water retention agent provided by the invention comprises 50.0-70.0 parts by weight of sodium acrylate, preferably 55.0-65.0 parts by weight of sodium acrylate, wherein the sodium acrylate is obtained by partially neutralizing acrylic acid with sodium carbonate, and the neutralization degree is preferably 65-75%.
The seaweed biological water retention agent provided by the invention comprises 0.02-0.04 parts by weight of N, N-methylene bisacrylamide; preferably comprises 0.022 to 0.036 parts by weight of N, N-methylenebisacrylamide; more preferably, it is contained in an amount of 0.024 to 0.030 parts by weight.
The seaweed biological water retention agent provided by the invention comprises 0.2-0.3 parts by weight of potassium persulfate; preferably from 0.21 to 0.24 parts by weight of potassium persulfate.
The seaweed biological water retention agent provided by the invention comprises 5.0-10.0 parts by weight of water; preferably, it includes 7.0 to 9.0 parts by weight.
The seaweed polysaccharide extract and the organic monomer are polymerized to form the seaweed biological water-retaining agent, so that the seaweed biological water-retaining agent has good water absorption and retention functions, and the problem of soil-crop drought is effectively solved.
The seaweed biological water-retaining agent is an agricultural and forestry water-retaining agent with uniform particles, biodegradability and high water absorption performance, and the water absorption capacity can reach 1118.32 g.g at most-1。
The product is safe and pollution-free, can absorb water with the weight of 300-1100 times, can achieve the water-saving effect of agriculture and forestry, has the characteristic of biodegradability, and solves the technical problems of water or nutrient loss of soil-crops and the like.
The invention provides a preparation method of the seaweed biological water-retaining agent in any one of the technical schemes, which comprises the following steps:
adding sodium acrylate with neutralization degree of 65-75%, N-methylene bisacrylamide, potassium persulfate and water into the seaweed polysaccharide extract obtained in the seaweed activating step, fully mixing, heating, stirring, drying and crushing to obtain the seaweed biological water-retaining agent.
The sources and preparation methods of the algal polysaccharide extract, sodium acrylate, N-methylene bisacrylamide, potassium persulfate and water are clearly described above, and are not described again.
The seaweed biological water-retaining agent with uniform particles and stable performance is prepared by taking seaweed polysaccharide extracts subjected to double enzymolysis as raw materials, heating sodium acrylate, N-methylene bisacrylamide, potassium persulfate and water in the seaweed polysaccharide extracts, stirring at a constant speed, mixing, drying and crushing.
Wherein the heating temperature is preferably 65-80 ℃, more preferably 68-78 ℃, and the heating time is preferably 2-4 h; more preferably 2.5 to 3.5 hours.
The method can realize the recycling of the seaweed resources, and the activated seaweed residues are used for producing other products in a workshop, thereby changing waste into valuable. The product has simple preparation process, easy operation and low cost, is beneficial to industrial production in workshops, and solves the problem that the technical achievement is easy to convert and the chain is broken and can not be applied industrially. The product is golden yellow particles, has the characteristics of high water absorption performance, strong reusability, high temperature resistance, high pressure resistance, freezing resistance, greenness, no pollution and the like, and has extremely wide application range, such as drought resistance, sand fixation, soil improvement and the like; in particular to improve the soil mass crack structure and the like.
The invention provides a seaweed biological water-retaining agent, which comprises the following raw materials in parts by weight: 30.0 to 60.0 portions of algal polysaccharide extract, 50.0 to 70.0 portions of sodium acrylate, 0.02 to 0.04 portion of N, N-methylene bisacrylamide, 0.2 to 0.3 portion of potassium persulfate and 5.0 to 10.0 portions of water. The seaweed polysaccharide extract and the monomer are polymerized to form the seaweed biological water-retaining agent, the product is safe and pollution-free, can absorb water with the weight of 300-fold and 1100-fold, can achieve the water-saving effect of agriculture and forestry, has the characteristic of biodegradability, and solves the technical problems of water or nutrient loss of soil-crops and the like.
In order to further illustrate the invention, the following will describe the seaweed biological water retention agent and the preparation method thereof in detail with reference to the examples.
Example 1
Preparing a raw material seaweed polysaccharide extract:
1) 150g of dried kelp meeting the requirements is selected, cleaned and coarsely crushed for later use.
2) Adding 20g of sodium citrate and 6g of protease, extracting and carrying out enzymolysis reaction for 2 hours at the temperature of 55 ℃.
3) To step 2) was further added 8g of sodium carbonate and the reaction was carried out at 75 ℃ for 3 hours.
4) 10g of citric acid is continuously added into the step 3), and the reaction is kept for 1 hour. Subsequently, 10g of boric acid, 5g of sodium acetate and 4g of sodium tripolyphosphate were added in this order, and the mixture was stirred and cooled naturally to 45 ℃.
5) Adding 300 ml of SE enzyme and 150 ml of FE enzyme into the step 4), carrying out enzymolysis for 2 hours at 45 ℃, and naturally cooling to normal temperature.
6) And (3) transferring the liquid obtained in the step 5) to a centrifuging, filtering and concentrating process, concentrating by 10 times, and discharging the solution to obtain the target extracting solution.
The algal polysaccharide extract prepared by the method is prepared by weighing 350 ml of sodium acrylate 450 ml with neutralization degree of 65%, 0.21 g of N, N-methylene bisacrylamide and 2.35 g of potassium persulfate, adding 50 ml of water, heating to 72 ℃, uniformly stirring, mixing for 2.5 hours, drying and crushing to obtain the algal biological water-retaining agent with uniform particles and stable performance, wherein the water absorption multiple of the product is as high as 800.
Example 2
Preparing a raw material seaweed polysaccharide extract:
1) selecting 300g of dry gulfweed which meets the requirement, cleaning, and coarsely crushing for later use.
2) Adding 45g of sodium citrate and 15g of protease, extracting and carrying out enzymolysis reaction for 2 hours at the temperature of 60 ℃.
3) To step 2) was further added 16g of sodium carbonate and the reaction was carried out at 75 ℃ for 3 hours.
4) 20g of citric acid is continuously added into the step 3), and the reaction is kept for 1 hour. Subsequently, 20g of boric acid, 12g of sodium acetate and 10g of sodium tripolyphosphate were added in this order, and the mixture was stirred and cooled naturally to 45 ℃.
5) Adding 600 ml of SE enzyme and 300 ml of FE enzyme into the step 4), carrying out enzymolysis for 2 hours at 45 ℃, and naturally cooling to normal temperature.
6) And (3) transferring the liquid obtained in the step 5) to a centrifuging, filtering and concentrating process, concentrating by 10 times, and discharging the solution to obtain the target extracting solution.
The algal polysaccharide extract prepared by the method is prepared by weighing 500 ml of sodium acrylate 650 ml with neutralization degree of 75%, 0.35 g of N, N-methylene bisacrylamide and 2.50 g of potassium persulfate, adding 90 ml of water, heating to 78 ℃, uniformly stirring, mixing for 3 hours, drying and crushing, and the prepared algal biological water-retaining agent with uniform particles and stable performance has water absorption multiple up to 1000.
Example 3
Preparing a raw material seaweed polysaccharide extract:
1) 1000g of dry kelp meeting the requirements is selected, cleaned and coarsely crushed for later use.
2) Adding 150g of sodium citrate and 60g of protease, extracting and carrying out enzymolysis reaction for 2 hours at the temperature of 60 ℃.
3) To step 2) was further added 70g of sodium carbonate and the reaction was carried out at 75 ℃ for 3 hours.
4) 70g of citric acid is continuously added into the step 3), and the reaction is kept for 1 hour. Subsequently, 90g of boric acid, 35g of sodium acetate and 25g of sodium tripolyphosphate were added in this order, and the mixture was stirred and cooled naturally to 45 ℃.
5) Adding 1500 ml of SE enzyme and 1000 ml of FE enzyme into the step 4), carrying out enzymolysis for 2 hours at 45 ℃, and naturally cooling to normal temperature.
6) And (3) transferring the liquid obtained in the step 5) to a centrifuging, filtering and concentrating process, concentrating by 10 times, and discharging the solution to obtain the target extracting solution.
2000 ml of algal polysaccharide extract prepared by the method is prepared, 1800 ml of sodium acrylate with the neutralization degree of 70%, 0.85 g of N, N-methylene bisacrylamide and 7.50 g of potassium persulfate are added with 300 ml of water, then the temperature is raised to 75 ℃, the mixture is stirred at a constant speed and is mixed for 3 hours, and the mixture is dried and crushed to prepare the algal biological water-retaining agent with uniform particles and stable performance, wherein the water absorption multiple of the product is as high as 1100.
Example 4 Effect verification of the seaweed biological water-retaining agent of the invention
1. Test design and determination
3 parts of seaweed biological water-retaining agent (example 1) were weighed out in 1g portions and placed in 2L of distilled water, respectively, for a measurement time of 12 hours. And (3) taking out every 1 hour, placing the water-retaining agent on a screen, draining excessive water, measuring the weight of the water-retaining agent, and calculating the water absorption multiplying power according to the formula (1) until the water absorption multiplying power is kept unchanged and the water-retaining agent is saturated.
Water absorption rate (mass of water-retaining agent after water absorption-original mass of water-retaining agent)/mass of dry water-retaining agent
2. Test results and analysis
The change of the water absorption capacity of the seaweed biological water retaining agent with time is shown in table 1. As can be seen from Table 1, the organic water-retaining agent is basically in a saturated state after standing in distilled water for 8 hours, the water absorption rate is basically kept unchanged along with the passage of time, and the water absorption capacity can reach 1118.32 g.g at most-1Compared with the original mass of the water-retaining agent of 1g, the weight is increased by over thousand times.
The change trend of the average water absorption capacity of the seaweed biological water retaining agent along with time is shown in figure 1. The trend of the curve in the figure shows that the water absorption rate of the water-retaining agent in the first 4 hours is relatively high, the quality of the water-retaining agent is basically kept unchanged after eight hours, and the water absorption multiple can reach thousand times. Therefore, the seaweed biological water retention agent has good water absorption performance.
TABLE 1 Water absorption Capacity as a function of time
Comparative example 1
Adding 50 ml of water into 80 ml of seaweed powder solution, 250 ml of sodium acrylate with the neutralization degree of 60%, 0.20 g of N, N-methylene bisacrylamide and 0.80 g of potassium persulfate, heating to 70 ℃, stirring at a constant speed, mixing for 2 hours, drying and crushing to obtain the seaweed powder biological water-retaining agent with rough particles, wherein the water absorption multiple of the product is 200.
Comparative example 2
100 ml of seaweed polysaccharide extract, 250 ml of sodium acrylate with the neutralization degree of 65%, 0.12 g of N, N-methylene bisacrylamide and 1.10 g of potassium persulfate are taken, 40 ml of water is added, then the temperature is raised to 72 ℃, the mixture is stirred at a constant speed for 2.5 hours, and the mixture is dried and crushed to prepare the seaweed biological water-retaining agent with the water absorption multiple of 300.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The seaweed biological water-retaining agent is characterized by comprising the following raw materials in parts by weight:
30.0 to 60.0 portions of algal polysaccharide extract, 50.0 to 70.0 portions of sodium acrylate, 0.02 to 0.04 portion of N, N-methylene bisacrylamide, 0.2 to 0.3 portion of potassium persulfate and 5.0 to 10.0 portions of water.
2. The seaweed biological water retention agent as claimed in claim 1, wherein the sodium acrylate is obtained by partially neutralizing acrylic acid with sodium carbonate, and the neutralization degree is 65-75%.
3. The seaweed biological water-retaining agent as claimed in claim 1, wherein the preparation method of the seaweed polysaccharide extract comprises the following steps:
a) cleaning and crushing seaweed to obtain a seaweed substrate;
b) mixing the sodium citrate-protease complex with a seaweed substrate, and carrying out enzymolysis to obtain an enzymolysis product;
c) mixing and extracting sodium carbonate and an enzymolysis product to obtain a primary extract;
d) mixing the primary extract with citric acid, boric acid, sodium acetate and sodium tripolyphosphate, and extracting to obtain seaweed extract;
e) mixing alginate lyase and fucoidin lyase with the seaweed extract, and carrying out enzymolysis to obtain a crude seaweed polysaccharide extract;
f) ultrafiltering the crude extract to obtain algal polysaccharide extract.
4. The seaweed biological water retaining agent as claimed in claim 3, wherein the seaweed is one or more of brown algae, red algae and green algae; the seaweed is naturally wild or artificially cultured.
5. The seaweed biological water retaining agent as claimed in claim 4, wherein the seaweed is one or more dry seaweed of brown algae; preferably, the seaweed is kelp.
6. The seaweed biological water-retaining agent as claimed in claim 3, wherein the enzymolysis temperature in step b) is 55-65 ℃; the enzymolysis time is 2-3 h;
the extraction temperature in the step c) is 75-85 ℃; the extraction time is 2-4 h;
the extraction temperature in the step d) is 55-65 ℃; the extraction time is 1-2 h, and the temperature is cooled to 40-50 ℃ after extraction;
the enzymolysis temperature of the step e) is 40-50 ℃; the enzymolysis time is 1-3 h;
and e) specifically, transferring the crude algal polysaccharide extract obtained in the step e) into ultrafiltration equipment, carrying out concentration operation, and removing macromolecular impurity components in a system for later use.
7. The seaweed biological water retaining agent as claimed in claim 3, wherein the mass ratio of the sodium citrate-protease complex to the seaweed substrate is (10-40): (1-20): (100-);
the mass ratio of the alginate lyase to the fucoidin lyase to the seaweed extract is (10-30): (5-20): (100-1000).
8. The seaweed biological water retention agent as claimed in claim 3, wherein the mass ratio of the extracting solution to the citric acid, the boric acid, the sodium acetate and the sodium tripolyphosphate is (100- & 400): (1-20): (1-30): (5-40): (1-20).
9. A preparation method of the seaweed biological water retention agent as described in any one of claims 1 to 8, characterized by comprising the following steps:
adding sodium acrylate with neutralization degree of 65-75%, N-methylene bisacrylamide, potassium persulfate and water into the seaweed polysaccharide extract obtained in the seaweed activating step, fully mixing, heating, stirring, drying and crushing to obtain the seaweed biological water-retaining agent.
10. The method according to claim 9, wherein the heating temperature is 65 to 80 ℃ and the heating time is 2 to 4 hours.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113773846A (en) * | 2021-09-09 | 2021-12-10 | 昆明理工大学 | Method for preparing agricultural water-retaining agent by using waste green algae |
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| CN112679664B (en) | 2022-07-19 |
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