CN112673104B - 新酯酶及其用途 - Google Patents

新酯酶及其用途 Download PDF

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Publication number
CN112673104B
CN112673104B CN201980057623.3A CN201980057623A CN112673104B CN 112673104 B CN112673104 B CN 112673104B CN 201980057623 A CN201980057623 A CN 201980057623A CN 112673104 B CN112673104 B CN 112673104B
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China
Prior art keywords
esterase
polyester
seq
amino acid
esterases
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CN201980057623.3A
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English (en)
Chinese (zh)
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CN112673104A (zh
Inventor
I·安德烈
V·图尼尔
A·吉勒
S·迪奎斯内
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Carbios SA
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Carbios SA
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201980057623.3A 2018-07-27 2019-07-26 新酯酶及其用途 Active CN112673104B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18306019.3 2018-07-27
EP18306019 2018-07-27
PCT/EP2019/070288 WO2020021117A1 (en) 2018-07-27 2019-07-26 Novel esterases and uses thereof

Publications (2)

Publication Number Publication Date
CN112673104A CN112673104A (zh) 2021-04-16
CN112673104B true CN112673104B (zh) 2025-02-25

Family

ID=63144938

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980057623.3A Active CN112673104B (zh) 2018-07-27 2019-07-26 新酯酶及其用途

Country Status (6)

Country Link
US (1) US11692181B2 (cg-RX-API-DMAC7.html)
EP (1) EP3830270A1 (cg-RX-API-DMAC7.html)
JP (1) JP7633149B2 (cg-RX-API-DMAC7.html)
KR (1) KR20210042122A (cg-RX-API-DMAC7.html)
CN (1) CN112673104B (cg-RX-API-DMAC7.html)
WO (1) WO2020021117A1 (cg-RX-API-DMAC7.html)

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CA2987842C (en) 2015-06-12 2024-01-02 Carbios Biodegradable polyester composition and uses thereof
PL3458508T3 (pl) 2016-05-19 2026-02-23 Carbios Sposób degradowania produktów z tworzyw sztucznych
CN116286728B (zh) 2016-07-12 2026-04-24 卡比奥斯公司 新酯酶及其用途
CN115927269A (zh) 2016-12-16 2023-04-07 卡比奥斯公司 增加塑料降解的蛋白酶
MX2020002290A (es) 2017-08-31 2020-07-13 Carbios Composicion liquida que comprende entidades biologicas y usos de la misma.
CA3084107A1 (en) 2017-12-21 2019-06-27 Carbios Novel proteases and uses thereof
EP4424823B1 (en) 2018-07-27 2025-12-03 Carbios Novel esterases and uses thereof
CN112639101B (zh) 2018-07-27 2025-03-18 卡比奥斯公司 新酯酶及其用途
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
US12492308B2 (en) 2019-03-28 2025-12-09 Carbios Multicomponent thermoplastic product
CN114096664B (zh) 2019-07-11 2025-03-07 卡比奥斯公司 新型酯酶及其用途
EP3997220B1 (en) 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
EP3838976B1 (en) 2019-12-19 2025-02-12 Carbios Process for degrading plastic products
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
JP2023546501A (ja) 2020-10-27 2023-11-02 キャルビオス 新規エステラーゼ及びその使用
DK181658B1 (en) * 2020-11-12 2024-09-11 Petroleo Brasileiro Sa Petrobras PROCESS FOR THE PRODUCTION OF GLYCOLIC ACID OR γ-HYDROXYBUTYRIC ACID INTEGRATED WITH THE BIOTECHNOLOGICAL DEPOLYMERISATION OF POLYETYLENE TEREPHTHALATE OR POLY(BUTYLENE SUCCINATE).
US20240228731A1 (en) 2021-05-20 2024-07-11 Carbios Process for degrading a plastic product comprising at least one polyester
CN118994721A (zh) 2021-05-20 2024-11-22 卡比奥斯公司 降解包含至少一种聚酯的塑料产品的方法
CN113698742B (zh) * 2021-08-31 2022-11-22 江南大学 一种聚对苯二甲酸乙二醇酯的改性方法及其在酶解过程中的应用
CN118355115A (zh) * 2021-11-16 2024-07-16 卡比奥斯公司 酯酶及其用途
WO2023088910A1 (en) * 2021-11-16 2023-05-25 Carbios Use of esterases
EP4433582A2 (en) * 2021-11-16 2024-09-25 Carbios Esterases and uses thereof
TW202411323A (zh) 2022-06-13 2024-03-16 法商卡爾畢歐斯公司 純化單乙二醇之方法
CN115197927B (zh) * 2022-08-09 2024-06-21 武汉新华扬生物股份有限公司 一种降解生物基塑料的复合酶制剂及其应用
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
CN115287274B (zh) * 2022-10-08 2023-01-06 中国农业科学院生物技术研究所 角质酶iccg的突变体及其应用
US20240286178A1 (en) 2023-01-09 2024-08-29 Protein Evolution Inc Systems and methods for enzymatic degradation of polymers, including copolymers, with a variety of enzymes
US20240352064A1 (en) 2023-03-21 2024-10-24 Protein Evolution Inc. Methods and compositions for the separation of particles from a fluid
KR20260011740A (ko) 2023-05-15 2026-01-23 까르비오 신규 에스테라제 및 이의 용도
KR20260011167A (ko) 2023-05-15 2026-01-22 까르비오 신규 에스테라제 및 이의 용도
CN117448297B (zh) * 2023-09-19 2024-12-03 源天生物科技(天津)有限公司 PET降解酶PET-mh183/208突变体及其编码基因、工程菌和应用
CN117187212B (zh) * 2023-09-19 2025-02-11 源天生物科技(天津)有限公司 PET降解酶PET-mh突变体及其编码基因、重组质粒、工程菌和应用
CN117264927B (zh) * 2023-09-19 2024-11-29 源天生物科技(天津)有限公司 PET降解酶PET-mh29/124/183突变体及其编码基因、工程菌和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
WO2015135757A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa Pet-esterasen und deren verwendung

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FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
TR201900782T4 (tr) 2012-11-20 2019-02-21 Carbios Plastik ürünlerin geri dönüştürülmesine yönelik yöntem.
US20160280881A1 (en) 2013-11-05 2016-09-29 Carbios A method for degrading a plastic
ES2924896T3 (es) 2014-05-16 2022-10-11 Carbios Procedimiento para reciclar artículos de plástico mixto con PET
EP3778883A1 (en) 2014-10-21 2021-02-17 Carbios Polypeptide having a polyester degrading activity and uses thereof
US10626242B2 (en) 2014-12-19 2020-04-21 Carbios Plastic compound and preparation process
CN107532153A (zh) 2015-03-13 2018-01-02 卡比奥斯公司 具有聚酯降解活性的新多肽及其用途
CA2987842C (en) 2015-06-12 2024-01-02 Carbios Biodegradable polyester composition and uses thereof
EP3394264A1 (en) 2015-12-21 2018-10-31 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
PL3458508T3 (pl) 2016-05-19 2026-02-23 Carbios Sposób degradowania produktów z tworzyw sztucznych
CN114891768B (zh) * 2016-07-12 2024-09-24 卡比奥斯公司 新酯酶及其用途
CN116286728B (zh) * 2016-07-12 2026-04-24 卡比奥斯公司 新酯酶及其用途
CN115927269A (zh) 2016-12-16 2023-04-07 卡比奥斯公司 增加塑料降解的蛋白酶
MX2020002290A (es) 2017-08-31 2020-07-13 Carbios Composicion liquida que comprende entidades biologicas y usos de la misma.
KR20230131950A (ko) 2017-08-31 2023-09-14 카르비오리스 효소를 포함하는 생분해성 폴리에스테르 물품
CA3084107A1 (en) 2017-12-21 2019-06-27 Carbios Novel proteases and uses thereof
CN112639101B (zh) 2018-07-27 2025-03-18 卡比奥斯公司 新酯酶及其用途
EP4424823B1 (en) 2018-07-27 2025-12-03 Carbios Novel esterases and uses thereof

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
WO2015135757A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa Pet-esterasen und deren verwendung

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Publication number Publication date
EP3830270A1 (en) 2021-06-09
JP2021531032A (ja) 2021-11-18
US11692181B2 (en) 2023-07-04
KR20210042122A (ko) 2021-04-16
US20210171921A1 (en) 2021-06-10
JP7633149B2 (ja) 2025-02-19
WO2020021117A1 (en) 2020-01-30
CN112673104A (zh) 2021-04-16

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