CN112656878A - Traditional Chinese medicine beauty liquid for removing freckles and whitening skin and preparation method thereof - Google Patents
Traditional Chinese medicine beauty liquid for removing freckles and whitening skin and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/26—Aristolochiaceae (Birthwort family), e.g. heartleaf
- A61K36/268—Asarum (wild ginger)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
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- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention relates to a traditional Chinese medicine beauty fluid for removing freckles and whitening skin and a preparation method thereof. The freckle-removing and whitening traditional Chinese medicine beauty fluid is prepared from the following raw materials in parts by weight: 15-30 parts of codonopsis pilosula, 15-30 parts of sophora flavescens, 3-10 parts of salvia miltiorrhiza, 3-10 parts of almond, 3-10 parts of radix paeoniae alba, 3-10 parts of motherwort, 3-10 parts of rhizoma typhonii, 3-10 parts of asarum and 3-10 parts of poria cocos. The invention provides the traditional Chinese medicine beauty liquid which can realize more whitening, effectively prevent pigmentation, remove chloasma and realize multi-technology cross production and the preparation method thereof, improves the quality and the competitiveness of the products and occupies more cosmetic markets.
Description
Technical Field
The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine beauty fluid for removing freckles and whitening skin and a preparation method thereof.
Background
The whitening image of 'skin like snow and gel like fat' is always an ideal situation for the classic women in China, and is also an aim for cumin of all modern oriental women and even women all over the world. However, the current pigmentation skin diseases, especially chloasma, are relatively commonly found on female consumers, and the image and the quality of life of female patients are seriously influenced.
Chloasma is commonly called butterfly spot and cyasma, belongs to the category of blackish spots in traditional Chinese medicine, mainly occurs in adolescence or after pregnancy of women, and is considered to be mainly caused by 1. excessive melanin formation caused by excessive synthesis reaction of facial melanin 2. poor metabolism of skin microcirculation and hypometabolism of melanin carried in blood, so that excessive accumulation of the facial melanin is caused.
Western medicine chemical synthesis is mostly adopted in products related to chloasma in the market, and although the products take effect quickly, the products are easy to relapse and have higher risk. However, the traditional Chinese medicine has obvious effect on treating chloasma by adopting traditional Chinese medicines, is not easy to relapse, and has gained more and more extensive attention and research, so the research and development of traditional Chinese medicine cosmetics for removing chloasma to whiten skin, especially traditional Chinese medicine beauty liquid with convenient use form, has become a popular subject of research in recent years.
According to investigation, most of the preparation processes of the traditional Chinese medicine beauty liquid developed in the market at present only adopt the leaching technology, and the traditional Chinese medicine leaching technology has the following defects: because the traditional Chinese medicine plant cells have cell walls with compact structures, the release of effective components in cell cytoplasm is blocked, so that the content of the active components in the extract is low, the molecular weight of the obtained active components is generally large, the active components are not easy to break through skin barriers and be absorbed, the utilization efficiency is low, and the whitening and freckle removing effects are influenced.
The advantages of the existing emerging traditional Chinese medicine fermentation technology are as follows: after the traditional Chinese medicinal materials are fermented by microorganisms, the cell walls of plant cells can be decomposed by various extracellular enzymes such as cellulase and the like generated in the metabolic process of the microorganisms, so that the cells of the traditional Chinese medicinal materials are broken, more effective components are dissolved out, and the content of the effective components is greatly improved. Meanwhile, various enzymes generated by microbial metabolism can reduce the molecular weight of effective components, and remove various macromolecular impurities, so that the macromolecular impurities can be better absorbed and utilized by a human body, thereby better reducing the synthesis of melanin and improving the skin microcirculation.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine beauty liquid for removing freckles and whitening skin.
The freckle-removing and whitening traditional Chinese medicine beauty liquid is prepared from the following raw materials in parts by weight:
15-30 parts of codonopsis pilosula, 15-30 parts of sophora flavescens, 3-10 parts of salvia miltiorrhiza, 3-10 parts of almond, 3-10 parts of radix paeoniae alba, 3-10 parts of motherwort, 3-10 parts of rhizoma typhonii, 3-10 parts of asarum and 3-10 parts of poria cocos.
The active ingredients of the freckle-removing and whitening traditional Chinese medicine beautifying liquid are prepared by extracting the raw materials by using 10-30% ethanol, and then implanting probiotics groups for fermentation.
The probiotic flora comprises: lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae, acetobacter, lactobacillus, acetic acid bacteria and yeast.
The preparation method of the active ingredients of the freckle-removing and whitening traditional Chinese medicine beauty fluid comprises the following steps:
A. pulverizing the above materials into coarse powder, extracting with 10-30% ethanol, and filtering to obtain Chinese medicinal extractive solution;
B. diluting the Chinese medicinal leaching solution with water to 2-4 times of the volume, sterilizing, adding 2-10 weight parts of brown sugar and 2-10 weight parts of honey, cooling to 25-30 deg.C, adding mixed bacteria solution of lactobacillus, acetic acid bacteria and yeast, fermenting, and filtering to obtain fermentation broth a;
C. and adding mixed bacteria liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus into the fermentation liquid a for fermentation, filtering and sterilizing to obtain the active ingredient extracting solution.
The ethanol extraction in the step A comprises the following steps:
extracting the crushed Chinese medicinal materials with 10-30% ethanol solution 10-20 times of the total weight of the Chinese medicinal materials for 30-50 days; wherein, the first 1-5 days, stirring for 2-5 times per day, standing for the rest time without stirring, and filtering to obtain Chinese medicinal extractive solution.
The fermentation in the step B comprises the following steps: adding the diluted solution of brown sugar and honey, cooling to 25-30 deg.C, implanting 105-108cfu/mL mixed bacteria solution of lactobacillus, acetic acid bacteria and yeast at a ratio of 1-2:1-2:1-2, and fermenting at 25-30 deg.C for 20-50 days.
The fermentation in the step C comprises the following steps: taking the fermentation liquid a, adding mixed bacterial liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus with the concentration of 105-; wherein, stirring is needed in the fermentation process, and the stirring speed is 30-80 rpm/min.
The freckle-removing and whitening traditional Chinese medicine beauty lotion also comprises the following auxiliary materials:
the auxiliary material group A comprises the following components in percentage by weight: water, propylene glycol, sodium polyacrylate, 6-15:0.5-1.5: 0.06-0.2;
the auxiliary material B group comprises the following components in percentage by weight: water, hydroxyethyl cellulose, methyl hydroxybenzoate, sodium hyaluronate, sclerotium rolfsii gum, propylene glycol and trehalose, wherein the weight ratio of the water to the hydroxyethyl cellulose to the sodium hydroxybenzoate to the sclerotium rolfsii gum is 50-80:0.02-0.1:0.05-0.15:0.05-0.15:0.02-0.1:0.5-1.5: 0.2-0.8;
and the auxiliary material group C comprises the following components in percentage by weight: beta-glucan: dipotassium glycyrrhizinate: 0.2-0.8:0.05-0.15:0.2-0.8 of phenoxyethanol;
in the freckle-removing and whitening traditional Chinese medicine beautifying liquid, the weight ratio of an active component extracting solution to an auxiliary material A group, an auxiliary material B group and an auxiliary material C group is as follows:
active component extracting solution: auxiliary materials A group: and auxiliary materials B group: the auxiliary material C is 5-15:50-60:20-40: 0.5-1.5.
The preparation method of the freckle-removing and whitening traditional Chinese medicine beauty fluid comprises the following steps:
A. uniformly mixing all the components in the auxiliary material group A to obtain a solution a;
B. mixing the components in the auxiliary material group C and the active component extracting solution uniformly to obtain a solution C;
C. mixing the components in the auxiliary material B group, stirring for 20-60 minutes at 85-90 ℃, and then homogenizing for 2-5 minutes to obtain a solution B;
D. adding the solution a into the solution b, stirring uniformly, controlling the temperature at 40-45 ℃, adding the solution c into the solution, and mixing uniformly to obtain the composite material.
The invention integrates the fermentation technology on the basis of the extraction technology, makes up the defects of the extraction technology, provides the traditional Chinese medicine beauty liquid which can realize more whitening, effectively prevent pigmentation, remove chloasma and multi-technology cross production and the preparation method thereof, improves the quality and the competitiveness of the products and occupies more cosmetic markets.
Drawings
FIG. 1 is a top view and a side view of the inhibitory effect of melanin on the body surface of a zebra fish embryo;
FIG. 2 is a comparison chart of the experimental groups for melanin inhibition;
FIG. 3 is a graph showing the comparison of the germination rates of the respective experimental groups;
FIG. 4 is a comparison of the angiogenesis lengths in each experimental group.
Detailed Description
The invention is further illustrated by the following examples. It should be understood that the examples of the present invention are for illustrative purposes and not intended to limit the present invention. Simple modifications of the invention in accordance with its spirit fall within the scope of the claimed invention. Unless otherwise indicated, the percentages of the amount of ethanol in the present invention are volume percentages, and v/v represents the volume ratio of the solution.
EXAMPLE 1 tablet
The raw materials comprise: 15kg of codonopsis pilosula, 15kg of sophora flavescens, 3kg of salvia miltiorrhiza, 3kg of almond, 3kg of white paeony root, 3kg of motherwort, 3kg of rhizoma typhonii, 3kg of asarum and 3kg of poria cocos.
Secondly, the preparation method of the active ingredients comprises the following steps:
A. pulverizing the medicinal materials into coarse powder, and extracting the pulverized medicinal materials with 10% ethanol solution of which the weight is 10 times of the total weight of the medicinal materials for 30 days; wherein, the stirring is carried out for 2 times on the 1 st day, the rest time is kept still without stirring, and then the filtration is carried out to obtain the traditional Chinese medicine extract.
B. Diluting the traditional Chinese medicine leaching liquor with water to 2 times of the volume of the traditional Chinese medicine leaching liquor, sterilizing, then adding 2kg of brown sugar and 2kg of honey, cooling to 25 ℃, implanting 105cfu/mL of mixed bacterial liquid of lactic acid bacteria, acetic acid bacteria and saccharomycetes, wherein the ratio of the lactic acid bacteria, the acetic acid bacteria and the saccharomycetes is 1:1:1, keeping the temperature at 25 ℃, fermenting for 20 days, and filtering to obtain fermentation liquor a;
C. adding mixed bacterial liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus with the concentration of 105cfu/mL into the fermentation liquid a, wherein the ratio of the lactobacillus plantarum, the lactobacillus acidophilus, the lactobacillus casei, the lactobacillus rhamnosus, the bifidobacterium longum, the saccharomyces cerevisiae and the bacillus aceticus is 1:1:1:1:1, and fermenting for 40 days at the temperature of 25 ℃; wherein, stirring is needed in the fermentation process, and the stirring speed is 30 rpm/min; then filtering the fermentation liquor, and sterilizing to obtain the active ingredient extract.
Thirdly, auxiliary material formula:
the auxiliary material group A comprises the following components in percentage by weight: water, propylene glycol, sodium polyacrylate, 6:0.5: 0.06;
the auxiliary material B group comprises the following components in percentage by weight: water, hydroxyethyl cellulose, methyl hydroxybenzoate, sodium hyaluronate, sclerotium rolfsii gum, propylene glycol and trehalose, wherein the weight ratio of the water to the hydroxyethyl cellulose to the sodium hydroxybenzoate to the trehalose is 50:0.02:0.05:0.05:0.02:0.5: 0.2;
and the auxiliary material group C comprises the following components in percentage by weight: beta-glucan: dipotassium glycyrrhizinate: phenoxyethanol 0.2:0.05: 0.2;
in the freckle-removing and whitening traditional Chinese medicine beautifying liquid, the weight ratio of an active component extracting solution to an auxiliary material A group, an auxiliary material B group and an auxiliary material C group is as follows:
active component extracting solution: auxiliary materials A group: and auxiliary materials B group: and the auxiliary material C comprises 5:50:20: 0.5. .
Fourthly, the preparation method of the freckle-removing and whitening traditional Chinese medicine beauty fluid comprises the following steps:
A. uniformly mixing all the components in the auxiliary material group A to obtain a solution a;
B. mixing the components in the auxiliary material group C and the active component extracting solution uniformly to obtain a solution C;
C. mixing the components in the auxiliary material B group, stirring for 20 minutes at 85 ℃, and then homogenizing for 2 minutes to obtain a solution B;
D. and adding the solution a into the solution b, uniformly stirring, controlling the temperature at 40 ℃, adding the solution c into the solution b, and uniformly mixing to obtain the composite material.
Example 2
The raw materials comprise: 30kg of codonopsis pilosula, 30kg of sophora flavescens, 10kg of salvia miltiorrhiza, 10kg of almond, 10kg of white paeony root, 10kg of motherwort, 10kg of giant typhonium rhizome, 10kg of asarum and 10kg of poria cocos.
Secondly, the preparation method of the active ingredients comprises the following steps:
A. pulverizing the medicinal materials into coarse powder, and extracting the pulverized medicinal materials with 30% ethanol solution 20 times of the total weight of the medicinal materials for 50 days; wherein, the first 5 days are stirred for 5 times every day, the rest time is kept still without stirring, and then the traditional Chinese medicine extract is obtained by filtering.
B. Diluting the traditional Chinese medicine leaching liquor with water to 4 times of the volume of the traditional Chinese medicine leaching liquor, sterilizing, then adding 10kg of brown sugar and 10kg of honey, cooling to 30 ℃, implanting 108cfu/mL of mixed bacterial liquid of lactic acid bacteria, acetic acid bacteria and saccharomycetes, wherein the ratio of the lactic acid bacteria, the acetic acid bacteria and the saccharomycetes is 2:1:2, fermenting for 50 days at 25-30 ℃, and filtering to obtain fermentation liquor a;
C. taking the fermentation liquor a, adding mixed bacterial liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus with the concentration of 108cfu/mL, wherein the ratio of the lactobacillus plantarum, the lactobacillus acidophilus, the lactobacillus casei, the lactobacillus rhamnosus, the bifidobacterium longum, the saccharomyces cerevisiae and the bacillus aceticus is 2:1:2:2:1:2:2, and fermenting for 80 days at the temperature of 30 ℃; wherein, stirring is needed in the fermentation process, and the stirring speed is 80 rpm/min; then filtering the fermentation liquor, and sterilizing to obtain the active ingredient extract.
Thirdly, auxiliary material formula:
the auxiliary material group A comprises the following components in percentage by weight: water, propylene glycol, sodium polyacrylate, 15:1.5: 0.2;
the auxiliary material B group comprises the following components in percentage by weight: water, hydroxyethyl cellulose, methyl hydroxybenzoate, sodium hyaluronate, sclerotium rolfsii gum, propylene glycol and trehalose, wherein the weight ratio of the water to the hydroxyethyl cellulose to the sodium hydroxybenzoate to the trehalose is 80:0.1:0.15:0.15:0.1:1.5: 0.8;
and the auxiliary material group C comprises the following components in percentage by weight: beta-glucan: dipotassium glycyrrhizinate: phenoxyethanol 0.8:0.15: 0.8;
in the freckle-removing and whitening traditional Chinese medicine beautifying liquid, the weight ratio of an active component extracting solution to an auxiliary material A group, an auxiliary material B group and an auxiliary material C group is as follows:
active component extracting solution: auxiliary materials A group: and auxiliary materials B group: and the auxiliary material C comprises 15:60:40: 1.5.
Fourthly, the preparation method of the freckle-removing and whitening traditional Chinese medicine beauty fluid comprises the following steps:
A. uniformly mixing all the components in the auxiliary material group A to obtain a solution a;
B. mixing the components in the auxiliary material group C and the active component extracting solution uniformly to obtain a solution C;
C. mixing the components in the auxiliary material B group, stirring for 60 minutes at 90 ℃, and then homogenizing for 5 minutes to obtain a solution B;
D. and adding the solution a into the solution b, stirring uniformly, controlling the temperature at 45 ℃, adding the solution c into the solution b, and mixing uniformly to obtain the composite material.
Example 3
The raw materials comprise: 20kg of codonopsis pilosula, 20kg of sophora flavescens, 6kg of salvia miltiorrhiza, 6kg of almond, 6kg of white paeony root, 6kg of motherwort, 6kg of rhizoma typhonii, 6kg of asarum and 6kg of poria cocos.
Secondly, the preparation method of the active ingredients comprises the following steps:
A. pulverizing the medicinal materials into coarse powder, and extracting the pulverized medicinal materials with 20% ethanol solution 15 times of the total weight of the medicinal materials for 40 days; wherein, the first 3 days, the mixture is stirred for 3 times every day, the rest time is kept still without stirring, and then the mixture is filtered to obtain the traditional Chinese medicine extract.
B. Diluting the traditional Chinese medicine leaching liquor by adding water to 3 times of the volume of the traditional Chinese medicine leaching liquor, sterilizing, then adding 6 parts by weight of brown sugar and 6 parts by weight of honey, cooling to 28 ℃, implanting 106cfu/mL of mixed bacterial liquid of lactic acid bacteria, acetic acid bacteria and saccharomycetes, wherein the ratio of the lactic acid bacteria, the acetic acid bacteria and the saccharomycetes is 1:2:1, fermenting for 30 days at 28 ℃, and filtering to obtain fermentation liquor a;
C. adding a mixed bacterial liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus with the concentration of 106cfu/mL into the fermentation liquid a, wherein the ratio of the lactobacillus plantarum, the lactobacillus acidophilus, the lactobacillus casei, the lactobacillus rhamnosus, the bifidobacterium longum, the saccharomyces cerevisiae and the bacillus aceticus is 1:2:1:2:1, and fermenting for 60 days at the temperature of 28 ℃; wherein, stirring is needed in the fermentation process, and the stirring speed is 60 rpm/min; then filtering the fermentation liquor, and sterilizing to obtain the active ingredient extract.
Thirdly, auxiliary material formula:
the auxiliary material group A comprises the following components in percentage by weight: water, propylene glycol, sodium polyacrylate, 10:1: 0.1;
the auxiliary material B group comprises the following components in percentage by weight: water, hydroxyethyl cellulose, methyl hydroxybenzoate, sodium hyaluronate, sclerotium rolfsii gum, propylene glycol and trehalose, wherein the weight ratio of the water to the hydroxyethyl cellulose to the sodium hydroxybenzoate to the trehalose is 60:0.06:0.1:0.1:0.06:1: 0.5;
and the auxiliary material group C comprises the following components in percentage by weight: beta-glucan: dipotassium glycyrrhizinate: phenoxyethanol 0.5:0.1: 0.5;
in the freckle-removing and whitening traditional Chinese medicine beautifying liquid, the weight ratio of an active component extracting solution to an auxiliary material A group, an auxiliary material B group and an auxiliary material C group is as follows:
active component extracting solution: auxiliary materials A group: and auxiliary materials B group: and the auxiliary material C comprises 10:55:30: 1.
Fourthly, the preparation method of the freckle-removing and whitening traditional Chinese medicine beauty fluid comprises the following steps:
A. uniformly mixing all the components in the auxiliary material group A to obtain a solution a;
B. mixing the components in the auxiliary material group C and the active component extracting solution uniformly to obtain a solution C;
C. mixing the components in the auxiliary material B group, stirring for 40 minutes at 88 ℃, and then homogenizing for 4 minutes to obtain a solution B;
D. and adding the solution a into the solution b, uniformly stirring, controlling the temperature at 43 ℃, adding the solution c into the solution b, and uniformly mixing to obtain the composite material.
Example 4
The raw materials comprise: 25Kg of codonopsis pilosula, 18Kg of sophora flavescens, 7Kg of salvia miltiorrhiza, 5Kg of almond, 8Kg of white paeony root, 4Kg of motherwort, 5Kg of rhizoma typhonii, 8Kg of asarum and 8Kg of poria cocos.
Secondly, the preparation method of the active ingredients comprises the following steps:
A. pulverizing the medicinal materials into coarse powder, and extracting the pulverized medicinal materials with 15% ethanol solution 12 times of the total weight of the medicinal materials for 35 days; wherein, the first 4 days are stirred for 3 times every day, the rest time is kept still without stirring, and then the traditional Chinese medicine extract is obtained by filtering.
B. Diluting the traditional Chinese medicine leaching liquor with water to 2 times of the volume of the traditional Chinese medicine leaching liquor, sterilizing, then adding 3kg of brown sugar and 8kg of honey, cooling to 30 ℃, implanting 108cfu/mL of mixed bacterial liquid of lactic acid bacteria, acetic acid bacteria and saccharomycetes, wherein the ratio of the lactic acid bacteria, the acetic acid bacteria and the saccharomycetes is 1:1:2, fermenting for 40 days at the temperature of 30 ℃, and filtering to obtain fermentation liquor a;
C. adding mixed bacterial liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus with the concentration of 105cfu/mL into the fermentation liquid a, wherein the ratio of the lactobacillus plantarum, the lactobacillus acidophilus, the lactobacillus casei, the lactobacillus rhamnosus, the bifidobacterium longum, the saccharomyces cerevisiae and the bacillus aceticus is 2:2:2:2:1:1,
fermenting at 25 deg.C for 50 days; wherein, stirring is needed in the fermentation process, and the stirring speed is 40 rpm/min; then filtering the fermentation liquor, and sterilizing to obtain the active ingredient extract.
Thirdly, auxiliary material formula:
the auxiliary material group A comprises the following components in percentage by weight: water, propylene glycol, sodium polyacrylate, 12:0.8: 0.15;
the auxiliary material B group comprises the following components in percentage by weight: water, hydroxyethyl cellulose, methyl hydroxybenzoate, sodium hyaluronate, sclerotium rolfsii gum, propylene glycol and trehalose, wherein the weight ratio of the water to the hydroxyethyl cellulose to the sodium hydroxybenzoate to the trehalose is 70:0.03:0.08:0.14:0.07:1.2: 0.3;
and the auxiliary material group C comprises the following components in percentage by weight: beta-glucan: dipotassium glycyrrhizinate: phenoxyethanol 0.3:0.12: 0.7;
in the freckle-removing and whitening traditional Chinese medicine beautifying liquid, the weight ratio of an active component extracting solution to an auxiliary material A group, an auxiliary material B group and an auxiliary material C group is as follows:
active component extracting solution: auxiliary materials A group: and auxiliary materials B group: and the auxiliary material C comprises 13:52:35: 0.8.
Fourthly, the preparation method of the freckle-removing and whitening traditional Chinese medicine beauty fluid comprises the following steps:
A. uniformly mixing all the components in the auxiliary material group A to obtain a solution a;
B. mixing the components in the auxiliary material group C and the active component extracting solution uniformly to obtain a solution C;
C. mixing the components in the auxiliary material B group, stirring for 30 minutes at 86 ℃, and then homogenizing for 5 minutes to obtain a solution B;
D. and adding the solution a into the solution b, uniformly stirring, controlling the temperature at 41 ℃, adding the solution c into the solution b, and uniformly mixing to obtain the composite material.
Example 5
The raw materials comprise: 17kg of codonopsis pilosula, 22kg of sophora flavescens, 7kg of salvia miltiorrhiza, 8kg of almond, 3kg of white paeony root, 6kg of motherwort, 8kg of rhizoma typhonii, 4kg of asarum and 8kg of poria cocos.
Secondly, the preparation method of the active ingredients comprises the following steps:
A. pulverizing the above materials into coarse powder, extracting with 10-30% ethanol solution 10-20 times of the total weight of the materials for 45 days; wherein, the first 4 days are stirred for 3 times every day, the rest time is kept still without stirring, and then the traditional Chinese medicine extract is obtained by filtering.
B. Diluting the Chinese medicinal leaching liquor with water to 3 times of the volume of the Chinese medicinal leaching liquor, sterilizing, adding 7kg and 6kg of honey, cooling to 29 ℃, implanting 107cfu/mL of mixed bacteria liquid of lactic acid bacteria, acetic acid bacteria and yeast, wherein the ratio of the lactic acid bacteria, the acetic acid bacteria and the yeast is 1:2:2, fermenting at 28 ℃ for 43 days, and filtering to obtain fermentation liquor a;
C. taking the fermentation liquor a, adding mixed bacterial liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus with the concentration of 106cfu/mL, wherein the ratio of the lactobacillus plantarum, the lactobacillus acidophilus, the lactobacillus casei, the lactobacillus rhamnosus, the bifidobacterium longum, the saccharomyces cerevisiae and the bacillus aceticus is 1:1:1:2:1:2:2,
fermenting at 27 deg.C for 72 days; wherein, stirring is needed in the fermentation process, and the stirring speed is 67 rpm/min; then filtering the fermentation liquor, and sterilizing to obtain the active ingredient extract.
Thirdly, auxiliary material formula:
the auxiliary material group A comprises the following components in percentage by weight: water, propylene glycol, sodium polyacrylate, 6-15:0.5-1.5: 0.06-0.2;
the auxiliary material B group comprises the following components in percentage by weight: water, hydroxyethyl cellulose, methyl hydroxybenzoate, sodium hyaluronate, sclerotium rolfsii gum, propylene glycol and trehalose, wherein the weight ratio of the water to the hydroxyethyl cellulose to the sodium hydroxybenzoate to the sclerotium rolfsii gum is 50-80:0.02-0.1:0.05-0.15:0.05-0.15:0.02-0.1:0.5-1.5: 0.2-0.8;
and the auxiliary material group C comprises the following components in percentage by weight: beta-glucan: dipotassium glycyrrhizinate: 0.2-0.8:0.05-0.15:0.2-0.8 of phenoxyethanol;
in the freckle-removing and whitening traditional Chinese medicine beautifying liquid, the weight ratio of an active component extracting solution to an auxiliary material A group, an auxiliary material B group and an auxiliary material C group is as follows:
active component extracting solution: auxiliary materials A group: and auxiliary materials B group: and the auxiliary material C comprises 7:56:23: 0.9.
Fourthly, the preparation method of the freckle-removing and whitening traditional Chinese medicine beauty fluid comprises the following steps:
A. uniformly mixing all the components in the auxiliary material group A to obtain a solution a;
B. mixing the components in the auxiliary material group C and the active component extracting solution uniformly to obtain a solution C;
C. mixing the components in the auxiliary material B group, stirring for 25 minutes at 85 ℃, and then homogenizing for 4 minutes to obtain a solution B;
D. and adding the solution a into the solution b, uniformly stirring, controlling the temperature at 41 ℃, adding the solution c into the solution b, and uniformly mixing to obtain the composite material.
Example 6 whitening and microcirculation efficacy evaluation test
Design of experiment group
1. Normal control group: no medicine is added;
2. ginkgo biloba leaf extract group:
the used medicines are as follows: diluting folium Ginkgo extract to 21.25 mg/ml;
3. example 3 low concentration group of chinese medicine leach liquor:
the used medicines are as follows: taking the leaching liquor of the traditional Chinese medicine obtained in the step A of the preparation method of the active ingredients in the embodiment 3, and diluting the leaching liquor of the traditional Chinese medicine to 21.25 mg/ml;
4. example 3 low concentration group of active ingredient extract:
the used medicines are as follows: taking the active ingredient extract in step C of the active ingredient preparation method of example 3, diluting the active ingredient extract to 21.25 mg/ml;
5. example 3 high-low concentration group of Chinese medicine leaching liquor:
the used medicines are as follows: taking the Chinese medicinal leaching liquor obtained in the step A of the preparation method of the active ingredients in the embodiment 3, and diluting the Chinese medicinal leaching liquor to 42.50 mg/ml;
6. example 3 high concentration group of active ingredient extract:
the used medicines are as follows: the active ingredient extract obtained in step C of the preparation method of active ingredients of example 3 was diluted to 42.50 mg/ml.
The amounts of each group are shown in table 1 below:
table 1 dosage design table for each experimental group
Second, skin whitening efficacy verification experiment
(I) Experimental method
1. Collecting AB line wild type zebra fish 24hpf embryos, grouping 20 embryos in each group (the specific grouping scheme is shown in table 1), and adding 5ml of medicine and 5ml of nutrient solution corresponding to the experimental group into each group for culture;
2. on day 3, observing the development state of melanin on the body surface of the embryo, selecting representative 2-3 tails from each group for photographing and recording, and carrying out quantitative analysis;
3. on 4 th to 6 th days, analyzing and processing data;
(II) Experimental observations of the Processes and results
The observation result of the melanin of the zebra fish is shown in figure 1, and the statistics of the melanin plaque area of the zebra fish in figure 1 can show that:
example 3 the low concentration group of the leaching liquor of traditional Chinese medicine has the melanin content reduced by 10 +/-1 percent compared with the normal control group, and the two groups have obvious difference (P < 0.01); example 3 the low concentration group of the active ingredient extract had a significantly different melanin content (22 ± 1)%, compared to the normal control group (P < 0.01). The content of melanin in the ginkgo biloba extract group (21.25mg/ml) is reduced by (61 +/-1)%, compared with that in the normal control group, the two groups have very obvious difference (P < 0.001); example 3 the content of melanin in the high and low concentration groups of the leaching liquor of traditional Chinese medicine is reduced by 47 +/-1% compared with that of the normal control group, and the two groups have very significant difference (P < 0.001); example 3 the high concentration group of the active ingredient extract had a very significant difference in melanin content (P <0.001) compared to the normal control group (59 ± 1)%.
Data statistics are shown in Table 2 below
TABLE 2 melanin content (mean. + -. SD) of the experimental groups
P <0.01, the low concentration group of the leaching solution of the traditional Chinese medicine in example 3 and the low concentration group of the extracting solution of the active ingredient in example 3 are significantly different from the normal control group; p <0.001, the ginkgo biloba extract group, the high and low concentration group of the Chinese herb leaching solution in example 3, and the high concentration group of the active ingredient extracting solution in example 3 were all significantly different from the normal control group.
Third, microcirculation efficacy verification
1. On the first day, the AB line transgenic zebrafish 24hpf embryos were collected and grouped into 20 embryos per group, the specific grouping scheme is shown in table 3. After grouping, except a normal control group, modeling by using an angiogenesis inhibitor, constructing a zebra fish microcirculation injury model, and then adding 5ml of medicine and 5ml of nutrient solution corresponding to an experimental group into each group of embryos for culturing;
2. on the third day, the development state of the embryo microcirculation is observed, each group selects representative 2-3 tails for fluorescence microscopic imaging, and quantitative analysis is carried out;
3. and 4-6 days, analyzing and processing data.
(II) Experimental observations of the Processes and results
1. Microcirculation efficacy verification
(1) The third day, the rate of deformity and mortality was counted. The embryo development of the ginkgo biloba extract group (21.25mg/ml), the Chinese medicinal leaching liquor low-concentration group in example 3 and the zebra fish low-concentration group in the active ingredient extracting solution in example 3 are basically normal without obvious deformity, and the length and the budding rate of the new blood vessel are increased compared with those of a normal control group; example 3 the high and low concentration groups of Chinese medicine leaching liquor and the high concentration group of active ingredient extracting solution in example 3 have basically normal development of zebra fish embryos without obvious deformity, and the length and the budding rate of new blood vessels are increased compared with those of the normal control group.
And (5) counting the budding rate of the zebra fish blood vessels of each group. The ginkgo biloba leaf extract group (21.25mg/ml) has an increased budding rate (94 +/-2)%, which is significantly different from the normal control group (P <0.001), the low concentration group of the leaching solution of the traditional Chinese medicine in the example 3 has an increased budding rate (64 +/-1)%, which is significantly different from the normal control group (P < 0.001); example 3 the low concentration group of the active ingredient extract increased the sprouting rate of (75 ± 1)%, compared to the normal control group, and the difference between them was very significant (P < 0.001). Example 3 the germination rate of the high and low concentration groups of the leaching liquor of traditional Chinese medicine is increased by (80 +/-1)%, and the two groups have very significant difference (P < 0.001); example 3 the germination rate of the active ingredient extract solution high concentration group was increased (92 ± 1)%, compared with the normal control group, and there was a very significant difference (P < 0.001).
Specific experimental data are shown in Table 3 below
TABLE 3 germination percentage (mean. + -. SD) for each experimental group
P <0.001, in example 3, the low concentration group of the leaching solution of the traditional Chinese medicine and the low concentration group of the extracting solution of the active ingredients in example 3 were very different from the normal control group; p <0.001, the ginkgo biloba extract group, the high-low concentration group of the Chinese medicinal leaching liquor in example 3, and the high concentration group of the active ingredient extracting solution in example 3 were significantly different from the normal control group.
The comparison result of the sprouting rate of each experimental group is shown in fig. 3, and the sprouting rate of the low-concentration group of the traditional Chinese medicine leaching liquor in the example 3 in fig. 3 is (164 +/-1)%,. P <0.001 of the normal control group; example 3 the sprouting rate of the low concentration group of the active ingredient extract was (175 ± 1)%, # P <0.001 of the normal control group. The sprouting rate of the ginkgo leaf extract group is (194 +/-2)%, and P is less than 0.001 of the normal control group; example 3 the sprouting rate of the high and low concentration groups of the leaching liquor of the traditional Chinese medicine is (180 plus or minus 1)%,. P <0.001 of the normal control group; example 3 the sprouting rate of the high concentration group of the active ingredient extract was (192 ± 1)%, # P <0.001 of the normal control group.
(2) The comparison result of the new blood vessel length of each experimental group is shown in figure 4, the ginkgo biloba extract group has the new blood vessel length increased (98 +/-1)%, and the two groups have obvious difference (P < 0.001); example 3 the length of the new blood vessel of the low concentration group of the leaching liquor of the traditional Chinese medicine is increased by (56 +/-1)%, and the two groups have obvious difference (P < 0.01); example 3 the low concentration group of the active ingredient extract increased the neovascular length (70 ± 1)%, compared to the normal control group, and the two groups were significantly different (P < 0.01). Example 3 the length of the new blood vessel of the high and low concentration groups of the leaching liquor of the traditional Chinese medicine is increased by (80 +/-10)%, and the two groups have very significant difference (P < 0.001); example 3 the high concentration group of active ingredient extract increased the neovascular length (95 ± 10)%, compared to the normal control group, and the two groups were very different (P < 0.001). Specific experimental data are shown in Table 4 below
TABLE 4 neovascular Length (mean. + -. SD) for each experimental group
P <0.01, in example 3, the low concentration group of the leaching solution of the traditional Chinese medicine and the low concentration group of the extracting solution of the active ingredients in example 3 were significantly different from the normal control group; p <0.001, the ginkgo biloba extract group, the high-low concentration group of the Chinese medicinal leaching liquor in example 3, and the high concentration group of the active ingredient extracting solution in example 3 were significantly different from the normal control group.
The angiogenesis length results of the experimental groups are shown in fig. 4, and the angiogenesis length of the low concentration group of the leaching liquor of the traditional Chinese medicine in the example 3 is (156 +/-1)%, P is less than 0.01 of the normal control group; example 3 angiogenesis length of the active ingredient extract low concentration group was (170 ± 1)%,. ap <0.01) of the normal control group. The angiogenesis length of the ginkgo biloba extract group is (198 +/-1)%, P is less than 0.001 of the normal control group; example 3 the angiogenesis length of the high and low concentration group of the leaching liquor of the traditional Chinese medicine is (180 plus or minus 1)%, +. P <0.001 of the normal control group; example 3 angiogenesis length of the active ingredient extract high concentration group was (195 ± 1)%, # P <0.001 of the normal control group.
Fourth, discuss and conclude
Samples of the ginkgo biloba extract group, the low-concentration group of the traditional Chinese medicine leaching solution in example 3, the low-concentration group of the active ingredient extracting solution in example 3, the high-concentration group of the traditional Chinese medicine leaching solution in example 3 and the high-concentration group of the active ingredient extracting solution in example 3 are respectively added into a 24hpf zebra fish embryo culture environment until the embryo grows to 72hpf, the embryo grows to be basically normal without obvious deformity and the body surface melanin is reduced compared with a normal control group, wherein the effect of the ginkgo biloba extract group is significantly different from that of the low-concentration group of the traditional Chinese medicine leaching solution in example 3 and the low-concentration group of the active ingredient extracting solution in example 3, and the low-concentration group of the traditional Chinese medicine leaching solution in example 3 and the low-concentration group of the active ingredient extracting solution in example 3, after all the angiogenesis inhibitors are used for molding, the angiogenesis length and the sprouting rate of blood vessels are increased compared with those of a normal control group. Example 3 the zebra fish embryos of the high and low concentration groups of the chinese medicine leach liquor and the high concentration group of the active ingredient extract in example 3 have substantially normal development without significant deformity, and the body surface melanin is reduced compared to the normal control group, wherein the high and low concentration groups of the chinese medicine leach liquor and the high concentration group of the active ingredient extract in example 3 have significant difference from the ginkgo biloba extract group, the high and low concentration groups of the chinese medicine leach liquor and the high concentration group of the active ingredient extract in example 3 have significant difference, and the high concentration group of the active ingredient extract in example 3 has significantly reduced effect compared to the high and low concentration group of the chinese medicine leach liquor in example 3, and the angiogenesis inhibitor is used for molding, so that the angiogenesis length and the sprouting rate of blood vessels are increased compared to the normal control group, wherein the high and low concentration groups of the chinese medicine leach liquor and the active ingredient extract in example 3 have no significant difference from the ginkgo biloba extract group, example 3 the effect of the high concentration group of active ingredient extract was significantly different from that of the high and low concentration group of chinese herb medicine extract of example 3, and the effect of the high concentration group of active ingredient extract of example 3 was more significantly reduced than that of the high and low concentration group of chinese herb medicine extract of example 3.
In conclusion, the fermented traditional Chinese medicine beauty liquid can effectively inhibit the generation of melanin, effectively promote the regeneration of capillary vessels and has the potential of improving the microcirculation of the blood vessels. The skin-beautifying liquid can effectively inhibit the melanin of the zebra fish embryo when the concentration is 21.25mg/ml and 42.50mg/ml, promotes the regeneration of the capillary vessels of the zebra fish embryo and the repair of microcirculation, and has more obvious effect than the leaching liquor before fermentation with the same concentration respectively, wherein the effect is better when the concentration is 42.50mg/ml, and has no obvious difference with the effect of a ginkgo leaf extract group, which indicates that the effect of the fermented skin-beautifying liquid is obviously enhanced compared with that of the beauty liquid before fermentation, and the effect can be positively correlated with the concentration in a certain range, namely, the higher the concentration is in the certain range, the more obvious the whitening and freckle-removing effect and the microcirculation effect are, and the further development value and significance are achieved.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (9)
1. The traditional Chinese medicine beauty liquid is characterized by being prepared from the following raw materials in parts by weight:
15-30 parts of codonopsis pilosula, 15-30 parts of sophora flavescens, 3-10 parts of salvia miltiorrhiza, 3-10 parts of almond, 3-10 parts of radix paeoniae alba, 3-10 parts of motherwort, 3-10 parts of rhizoma typhonii, 3-10 parts of asarum and 3-10 parts of poria cocos.
2. The traditional Chinese medicine beauty liquid for removing freckles and whitening as claimed in claim 1, characterized in that the active ingredients are prepared by extracting each raw material with 10-30% ethanol, and then implanting probiotics groups for fermentation.
3. The traditional Chinese medicine beauty liquid for removing freckles and whitening skin according to claim 2, wherein the probiotic flora comprises: lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae, acetobacter, lactobacillus, acetic acid bacteria and yeast.
4. The traditional Chinese medicine beauty liquid for removing freckles and whitening skin, according to claim 1, is characterized in that the preparation method of the active ingredients comprises the following steps:
A. pulverizing the above materials into coarse powder, extracting with 10-30% ethanol, and filtering to obtain Chinese medicinal extractive solution;
B. diluting the Chinese medicinal leaching solution with water to 2-4 times of the volume, sterilizing, adding 2-10 weight parts of brown sugar and 2-10 weight parts of honey, cooling to 25-30 deg.C, adding mixed bacteria solution of lactobacillus, acetic acid bacteria and yeast, fermenting, and filtering to obtain fermentation broth a;
C. and adding mixed bacteria liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus into the fermentation liquid a for fermentation, filtering and sterilizing to obtain the active ingredient extracting solution.
5. The traditional Chinese medicine beauty liquid for removing freckles and whitening skin according to claim 4, which is characterized in that:
the ethanol extraction in the step A comprises the following steps:
extracting the crushed Chinese medicinal materials with 10-30% ethanol solution 10-20 times of the total weight of the Chinese medicinal materials for 30-50 days; wherein, the first 1-5 days, stirring for 2-5 times per day, standing for the rest time without stirring, and filtering to obtain Chinese medicinal extractive solution.
6. The traditional Chinese medicine beauty liquid for removing freckles and whitening skin according to claim 4, which is characterized in that:
the fermentation in the step B comprises the following steps: adding the diluted solution of brown sugar and honey, cooling to 25-30 deg.C, implanting 105-108cfu/mL mixed bacteria solution of lactobacillus, acetic acid bacteria and yeast at a ratio of 1-2:1-2:1-2, and fermenting at 25-30 deg.C for 20-50 days.
7. The traditional Chinese medicine beauty liquid for removing freckles and whitening skin according to claim 4, which is characterized in that:
the fermentation in the step C comprises the following steps: taking the fermentation liquid a, adding mixed bacterial liquid of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, bifidobacterium longum, saccharomyces cerevisiae and bacillus aceticus with the concentration of 105-; wherein, stirring is needed in the fermentation process, and the stirring speed is 30-80 rpm/min.
8. The traditional Chinese medicine beauty liquid for removing freckles and whitening skin, according to claim 4, is characterized by further comprising the following auxiliary materials:
the auxiliary material group A comprises the following components in percentage by weight: water, propylene glycol, sodium polyacrylate =6-15:0.5-1.5: 0.06-0.2;
the auxiliary material B group comprises the following components in percentage by weight: water, hydroxyethyl cellulose, methyl hydroxybenzoate, sodium hyaluronate, sclerotium rolfsii gum, propylene glycol and trehalose =50-80:0.02-0.1:0.05-0.15: 0.05-0.15:0.02-0.1:0.5-1.5: 0.2-0.8;
and the auxiliary material group C comprises the following components in percentage by weight: beta-glucan: dipotassium glycyrrhizinate: phenoxyethanol =0.2-0.8:0.05-0.15: 0.2-0.8;
in the freckle-removing and whitening traditional Chinese medicine beautifying liquid, the weight ratio of an active component extracting solution to an auxiliary material A group, an auxiliary material B group and an auxiliary material C group is as follows:
active component extracting solution: auxiliary materials A group: and auxiliary materials B group: and the auxiliary material C group =5-15:50-60:20-40: 0.5-1.5.
9. The traditional Chinese medicine beauty liquid for removing freckles and whitening skin, according to claim 8, is characterized in that the preparation method comprises the following steps:
A. uniformly mixing all the components in the auxiliary material group A to obtain a solution a;
B. mixing the components in the auxiliary material group C and the active component extracting solution uniformly to obtain a solution C;
C. mixing the components in the auxiliary material B group, stirring for 20-60 minutes at 85-90 ℃, and then homogenizing for 2-5 minutes to obtain a solution B;
D. adding the solution a into the solution b, stirring uniformly, controlling the temperature at 40-45 ℃, adding the solution c into the solution, and mixing uniformly to obtain the composite material.
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| CN113288847A (en) * | 2021-05-25 | 2021-08-24 | 上海应用技术大学 | Preparation method and application of rhizoma typhonii fermentation extracting solution |
| CN114159545A (en) * | 2021-12-29 | 2022-03-11 | 北京远胜达生物科技发展有限公司 | Whitening anti-wrinkle composition containing enzyme and application of composition in medicines |
| CN115634186A (en) * | 2022-11-04 | 2023-01-24 | 广州市暨源生物科技有限公司 | Plant combined fermentation product with yellow dispelling effect and preparation method and application thereof |
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| CN1046088C (en) * | 1992-12-19 | 1999-11-03 | 管遵信 | Chinese-medicine beauty liquor and producing process thereof |
| CN107468999A (en) * | 2017-08-25 | 2017-12-15 | 广州蛭肽素医药科技有限公司 | A kind of fermentation composition with beauty treatment and eliminating spot function and preparation method thereof |
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| CN113288847A (en) * | 2021-05-25 | 2021-08-24 | 上海应用技术大学 | Preparation method and application of rhizoma typhonii fermentation extracting solution |
| CN114159545A (en) * | 2021-12-29 | 2022-03-11 | 北京远胜达生物科技发展有限公司 | Whitening anti-wrinkle composition containing enzyme and application of composition in medicines |
| CN115634186A (en) * | 2022-11-04 | 2023-01-24 | 广州市暨源生物科技有限公司 | Plant combined fermentation product with yellow dispelling effect and preparation method and application thereof |
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Application publication date: 20210416 |