CN112652360A - Digital PCR fluorescent signal acquisition method, device, equipment and storage medium - Google Patents

Digital PCR fluorescent signal acquisition method, device, equipment and storage medium Download PDF

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Publication number
CN112652360A
CN112652360A CN202011482833.8A CN202011482833A CN112652360A CN 112652360 A CN112652360 A CN 112652360A CN 202011482833 A CN202011482833 A CN 202011482833A CN 112652360 A CN112652360 A CN 112652360A
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China
Prior art keywords
chip
linear array
array camera
digital pcr
acquired
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CN202011482833.8A
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Chinese (zh)
Inventor
杨进
黄培
杨仁杰
胡杰
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Suzhou Dianan Biotechnology Co ltd
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Suzhou Dianan Biotechnology Co ltd
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Priority to CN202011482833.8A priority Critical patent/CN112652360A/en
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/60—Control of cameras or camera modules
    • H04N23/695—Control of camera direction for changing a field of view, e.g. pan, tilt or based on tracking of objects
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N25/00—Circuitry of solid-state image sensors [SSIS]; Control thereof
    • H04N25/70—SSIS architectures; Circuits associated therewith
    • H04N25/701—Line sensors
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image

Abstract

The embodiment of the invention discloses a method, a device, equipment and a storage medium for acquiring a digital PCR fluorescent signal. The method comprises the following steps: adjusting the imaging surface of the linear array camera to be parallel to the area of the line to be acquired on the chip; the linear array camera acquires an image of a line area to be acquired of the chip and controls the relative motion of the chip and the linear array camera; the image is data processed to obtain a digital PCR fluorescence signal. The embodiment of the invention solves the problem that the installation and debugging processes of the camera are complex and fussy in the prior art, and can carry out synchronous data processing while acquiring images.

Description

Digital PCR fluorescent signal acquisition method, device, equipment and storage medium
Technical Field
The embodiment of the invention relates to an information acquisition technology, in particular to a method, a device, equipment and a storage medium for acquiring a digital PCR fluorescent signal.
Background
The digital PCR is a new method for nucleic acid detection and quantitative analysis, can obtain higher detection precision, has small detection deviation, and can realize absolute quantification and detection of rare allele. Digital PCR generally involves several stages of droplet dispersion, amplification, signal acquisition and statistical analysis. Acquisition of digital PCR droplet fluorescence signals is part of this signal acquisition step.
An area-array camera is mostly adopted in the market at present, liquid drops in a square fluid chip groove are mostly photographed at one time, the camera and the chip are also moved relatively, images are photographed for multiple times, then an image is spliced, and then image data processing is carried out. After image acquisition, a series of image processing including preprocessing, denoising, liquid drop segmentation, liquid drop center calculation, size calculation, roundness calculation, impurity removal and the like are required, and the calculation workload is large.
Disclosure of Invention
The embodiment of the invention provides a method, a device, equipment and a storage medium for acquiring a digital PCR fluorescent signal, which are used for solving the defects of complex installation and debugging of a camera and complex image splicing and subsequent image processing after image acquisition in the prior art, realizing accurate and efficient image acquisition and processing and obtaining the digital PCR fluorescent signal.
In a first aspect, an embodiment of the present invention provides a digital PCR fluorescence signal acquisition method, including:
adjusting the imaging surface of the linear array camera to be parallel to the area of the line to be acquired on the chip;
the linear array camera acquires an image of the line area to be acquired and controls the relative motion of the chip and the linear array camera;
and performing data processing on the image to acquire a digital PCR fluorescent signal.
Optionally, adjusting that the imaging surface of the linear array camera is parallel to the region of the line to be acquired on the chip includes:
placing the chip on a bottom plate;
adjusting the linear array camera to rotate along a first direction so that an imaging surface of the linear array camera is parallel to a plane where a chip is located;
the first direction is parallel to the linear array arrangement direction of the linear array camera and is parallel to the plane of the bottom plate.
Optionally, the controlling the relative motion between the chip and the line camera includes:
controlling the chip and/or the linear array camera to translate relatively;
the linear array camera synchronously acquires an image of the chip once every time the linear array camera translates relatively; and repeating the process until all images of the chip are acquired.
Optionally, the processing the image to obtain the digital PCR fluorescence signal includes:
and after at least two images of the chip are collected, the computer reads the images once, and data processing is carried out on the obtained images to obtain digital PCR fluorescent signals.
In a second aspect, an embodiment of the present invention provides a digital PCR fluorescence signal acquiring apparatus, including:
the adjusting module is used for adjusting the imaging surface of the linear array camera to be parallel to the area of the line to be acquired on the chip;
the acquisition module is used for controlling the linear array camera to acquire the image of the chip;
the control module is used for controlling the relative motion of the chip and the linear array camera;
and the processing module is used for carrying out data processing on the image so as to obtain a digital PCR fluorescent signal.
Optionally, the adjusting module is specifically configured to:
placing the chip on a bottom plate;
adjusting the linear array camera to rotate along a first direction so that an imaging surface of the linear array camera is parallel to a line region to be acquired on a chip;
the first direction is parallel to the linear array arrangement direction of the linear array camera and is parallel to the plane of the bottom plate.
Optionally, the control module is specifically configured to:
and controlling the chip and/or the linear array camera to translate relatively.
Optionally, the processing module is specifically configured to:
and reading the image of the chip once by the computer every time at least two times of acquisition, and processing the acquired image to acquire a digital PCR fluorescent signal.
In a third aspect, an embodiment of the present invention provides a digital PCR fluorescence signal acquiring apparatus, including:
one or more processors;
storage means for storing one or more programs;
when executed by the one or more processors, cause the one or more processors to implement the digital PCR fluorescence signal acquisition method.
In a fourth aspect, the embodiment of the present invention further provides a computer-readable storage medium, on which a computer program is stored, where the computer program is executed by a processor to implement the digital PCR fluorescence signal acquiring method.
The method for acquiring the digital PCR fluorescent signal provided by the embodiment of the invention comprises the following steps: adjusting the imaging surface of the linear array camera to be parallel to the area of the line to be acquired on the chip; the linear array camera obtains an image of a line area to be acquired on a chip; controlling the relative motion of the chip and the linear array camera, and simultaneously acquiring an image of the chip by the linear array camera; the image is data processed to obtain a digital PCR fluorescence signal. The method comprises the steps of adjusting the imaging surface of a linear array camera to be parallel to a line area to be acquired on a chip, acquiring high-quality images by using the linear array camera, controlling the relative motion of the chip and the linear array camera, acquiring the images of the chip by using the linear array camera and synchronously processing the images, solving the problem that the camera installation and debugging process is complex and tedious in the prior art, synchronously processing the data during image acquisition, avoiding splicing and subsequent image processing work, and efficiently and accurately acquiring digital PCR fluorescent signals.
Drawings
To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, a brief description will be given below of the drawings required for the embodiments or the technical solutions in the prior art, and it is obvious that the drawings in the following description, although being some specific embodiments of the present invention, can be extended and extended to other structures and drawings by those skilled in the art according to the basic concepts of the device structure, the driving method and the manufacturing method disclosed and suggested by the various embodiments of the present invention, without making sure that these should be within the scope of the claims of the present invention.
FIG. 1 is a schematic flow chart of a method for acquiring a digital PCR fluorescent signal according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a scanning system of a line-array camera according to an embodiment of the present invention;
FIG. 3 is a schematic flow chart of a method for acquiring a digital PCR fluorescent signal according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a digital PCR fluorescent signal acquiring apparatus according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a digital PCR fluorescence signal acquiring apparatus according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described through embodiments with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the basic idea disclosed and suggested by the embodiments of the present invention, are within the scope of the present invention.
Example one
Fig. 1 is a schematic flow chart of a digital PCR fluorescent signal acquiring method according to an embodiment of the present invention, where the present embodiment is applicable to acquiring a digital PCR fluorescent signal through linear array camera image acquisition and analysis processing, and the method may be executed by a digital PCR fluorescent signal acquiring apparatus, and the apparatus may be implemented in a hardware and/or software manner. The method specifically comprises the following steps:
step 110, adjusting an imaging surface of the linear array camera to be parallel to a line area to be acquired on a chip;
fig. 2 is a schematic structural diagram of a scanning system of a line camera according to an embodiment of the present invention, in which a line camera 7 and a chip 9 are installed and installed in the scanning system of the line camera, the scanning system of the line camera further includes a bottom plate 1, a linear slide rail 2, a micro slider 3, a linear stepper motor 4, a slider 5, a cross beam 6 and a camera adjusting structure 8, the line camera 7 and the camera adjusting structure 8 are disposed on the cross beam 6, the cross beam 6 is disposed on the linear slide rail 2, the micro slider 3 and the slider 5 control the line camera to move along an X-axis direction, the linear stepper motor 4 can drive the line camera 7 to rotate at a fixed angle step by step, so as to implement accurate control, and meanwhile, the debugging of the line camera 7 can ensure that the line area to be acquired on the chip 9 is parallel only by fine-.
Specifically, the chip 9 may be a microfluidic chip, and is composed of components such as a channel and a reactor for micron-sized fluid, and the structure thereof greatly increases the area to volume ratio of the fluid environment, and utilizes the special performance related to the object indication to the maximum extent, thereby realizing the whole information collection on one chip.
Step 120, the linear array camera acquires an image of a line area to be acquired, and controls the relative motion of the chip and the linear array camera;
the linear array camera collects images which are two-dimensional images, the pixel resolution in the length direction of the images even reaches dozens of K pixels, the pixel resolution in the width direction is only several K pixels, the pixel quantity contained in one linear direction is more than that of the traditional area array camera, and the resolution of image collection on a chip is better guaranteed.
The relative movement of the chip and the linear array camera and the image acquisition are carried out synchronously, the relative movement of the chip 9 and the linear array camera 7 comprises the movement of the control chip 7, the movement of the linear array camera 9 is controlled or the simultaneous movement of the control chip 9 and the linear array camera 7 is controlled, and the relative movement can be flexibly selected according to actual conditions during specific implementation.
Preferably, only the chip 9 is moved at a constant speed, and the linear array camera 7 is used for continuous scanning, so as to achieve uniform detection of the whole chip surface.
Step 130, processing data of each image to obtain a digital PCR fluorescent signal;
the acquired image is transmitted to a computer connected with the linear array camera through a data line to be processed.
According to the technical scheme, the imaging surface of the linear array camera is parallel to the plane where the chip is located by adjusting, the relative movement of the chip and the linear array camera is controlled, the relative movement comprises the movement of the control chip, the linear array camera is controlled to move, the control chip and the linear array camera move simultaneously, the image of the chip is acquired after multiple times of relative movement every time, the image is read once through a computer and is synchronously processed, the complex and tedious problems of common camera installation and debugging in the prior art are solved, synchronous data processing can be performed while image acquisition is performed, splicing and subsequent image processing work are not needed, and digital PCR fluorescent signal acquisition is efficiently and accurately achieved.
Example two
With continued reference to fig. 1 and fig. 2, in this embodiment, on the basis of the foregoing embodiment, it is preferable to further optimize the adjustment of the imaging plane of the line camera 7 and the region of the line to be acquired on the chip 9 in a horizontal manner, that is, to place the chip 9 on the bottom plate 1, and adjust the line camera 7 to rotate in the first direction, so that the imaging plane of the line camera 7 is parallel to the region to be acquired on the chip 1;
wherein, the first direction rotation is along the X axle direction shown in fig. 2 promptly, is on a parallel with the linear array arrangement direction of linear array camera, and is on a parallel with the rotation of bottom plate 1 place plane, compares traditional area array camera and need carry out X, Y two direction adjustments of axle, guarantees that camera and whole chip plane are parallel, and the loaded down with trivial details process of camera installation and debugging is reduced to very big degree.
According to the technical scheme, the chip is horizontally placed on the bottom plate, the linear array camera can adjust the imaging surface of the linear array camera to be parallel to the area of the line to be acquired on the chip only by adjusting the rotation of the first direction, the problem that the plane of the camera is parallel to the plane of the chip only by adjusting two axial rotation angles in the installation of the traditional planar array camera is solved, and the effect of efficiently and quickly realizing the parallel of the imaging surface of the linear array camera and the plane of the chip is achieved.
EXAMPLE III
Fig. 3 is a schematic flow chart of a digital PCR fluorescent signal acquisition method according to a third embodiment of the present invention, where the third embodiment is applicable to a case where a digital PCR fluorescent signal is acquired by a linear array camera and a chip moving relatively, the method of the third embodiment includes:
step 210, adjusting an imaging surface of the linear array camera to be parallel to a line area to be acquired on a chip;
step 220, the linear array camera acquires an image of a line area to be acquired, and simultaneously the chip and/or the linear array camera is controlled to move horizontally relatively; the linear array camera synchronously acquires an image of the chip once every time the linear array camera translates relatively; repeating the above process until all images of the chip are obtained;
the linear array camera carries out progressive continuous scanning in the process of acquiring images so as to uniformly detect the whole surface of the linear array camera.
And step 230, reading the image of the chip once by the computer every time the image of the chip is acquired at least twice, and processing the acquired image to acquire a digital PCR fluorescent signal.
And when the images of the chip are acquired at least twice, the images are synchronously transmitted to a computer for image processing, and all the images are all recorded in one picture.
The technical scheme of this embodiment, through image acquisition and data processing get synchronous going on, solved prior art area array camera image acquisition back image need splice the aftertreatment, have the problem in concatenation gap, reached the strong high-efficient convenient effect of flexibility.
Example four
Fig. 4 is a schematic structural diagram of an apparatus for acquiring a digital PCR fluorescence signal according to an embodiment of the present invention, the apparatus includes:
the adjusting module 310 is used for adjusting the imaging surface of the linear array camera to be parallel to the area of the line to be acquired of the chip;
the acquisition module 320 is used for controlling the linear array camera to acquire an image of a line area to be acquired;
the control module 330 is used for controlling the relative motion of the chip and the line camera;
and the processing module 340 is configured to perform data processing on the image to obtain a digital PCR fluorescence signal.
According to the embodiment of the invention, the imaging surface of the linear array camera is adjusted to be parallel to the line area to be acquired on the chip, the relative movement of the chip and the linear array camera is controlled, the image of the chip is synchronously acquired, the acquired image is subjected to image processing, and the digital PCR fluorescent signal is acquired, so that the accuracy and the high efficiency of acquiring the digital PCR fluorescent signal are realized, and the problems that the camera in the prior art is complicated to install and debug and needs to acquire the image and finish unified image processing are solved.
Optionally, the adjusting module 310 is specifically configured to:
placing the chip on a bottom plate; adjusting the linear array camera to rotate along a first direction so that an imaging surface of the linear array camera is parallel to a line region to be acquired on a chip; the first direction is parallel to the linear array arrangement direction of the linear array camera and is parallel to the plane of the bottom plate.
Optionally, the control module 330 is specifically configured to:
controlling the chip and/or the linear array camera to move horizontally relatively;
optionally, the processing module 340 is specifically configured to:
and reading the image of the chip once by the computer every time at least two times of acquisition, and processing the acquired image to acquire a digital PCR fluorescent signal.
The digital PCR fluorescent signal acquisition device provided by the embodiment of the invention can execute the digital PCR fluorescent signal acquisition method provided by any embodiment of the invention, and has corresponding functional modules and beneficial effects of the execution method.
EXAMPLE five
Fig. 5 is a schematic structural diagram of a digital PCR fluorescence signal acquiring apparatus according to a fifth embodiment of the present invention, as shown in fig. 5, the apparatus includes a processor 40, a memory 41, an input device 42, and an output device 43; the number of processors 40 in the device may be one or more, and one processor 40 is taken as an example in fig. 5; the processor 40, the memory 41, the input device 42 and the output device 43 in the apparatus may be connected by a bus or other means, which is exemplified in fig. 5.
The memory 41 serves as a computer-readable storage medium for storing software programs, computer-executable programs, and modules, such as modules corresponding to a digital PCR fluorescence signal acquisition method in the embodiments of the present invention (for example, the adjusting module 310, the acquiring module 320, the control module 330, and the processing module 340 in a digital PCR fluorescence signal acquisition apparatus). The processor 40 executes various functional applications and data processing of the device by executing software programs, instructions and modules stored in the memory 41, so as to realize one of the above-mentioned digital PCR fluorescence signal acquisition methods.
The memory 41 may mainly include a storage program area and a storage data area, wherein the storage program area may store an operating system, an application program required for at least one function; the storage data area may store data created according to the use of the terminal, and the like. Further, the memory 41 may include high speed random access memory, and may also include non-volatile memory, such as at least one magnetic disk storage device, flash memory device, or other non-volatile solid state storage device. In some examples, memory 41 may further include memory located remotely from processor 40, which may be connected to the device over a network. Examples of such networks include, but are not limited to, the internet, intranets, local area networks, mobile communication networks, and combinations thereof.
The input device 42 is operable to receive input numeric or character information and to generate key signal inputs relating to user settings and function controls of the apparatus/device. The output device 43 may include a display device such as a display screen.
Example six:
an embodiment of the present invention further provides a storage medium containing computer-executable instructions, which when executed by a computer processor, perform a search method, the method including:
adjusting the imaging surface of the linear array camera to be parallel to the area of the line to be acquired on the chip;
the linear array camera acquires an image of a line area to be acquired and controls the relative motion of the chip and the linear array camera;
the image is data processed to obtain a digital PCR fluorescence signal.
Of course, the storage medium provided by the embodiment of the present invention contains computer-executable instructions, and the computer-executable instructions are not limited to the method operations described above, and may also perform related operations in the search method provided by any embodiment of the present invention.
From the above description of the embodiments, it is obvious for those skilled in the art that the present invention can be implemented by software and necessary general hardware, and certainly, can also be implemented by hardware, but the former is a better embodiment in many cases. Based on such understanding, the technical solutions of the present invention may be embodied in the form of a software product, which can be stored in a computer-readable storage medium, such as a floppy disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a FLASH Memory (FLASH), a hard disk or an optical disk of a computer, and includes several instructions for enabling a computer device (which may be a personal computer, a server, or a network device) to execute the methods according to the embodiments of the present invention.
It should be noted that, in the embodiment of the above search apparatus, each included unit and module are merely divided according to functional logic, but are not limited to the above division as long as the corresponding functions can be implemented; in addition, specific names of the functional units are only for convenience of distinguishing from each other, and are not used for limiting the protection scope of the present invention.
It is to be noted that the foregoing is only illustrative of the preferred embodiments of the present invention and the technical principles employed. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious modifications, rearrangements, combinations and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail by the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims (10)

1. A digital PCR fluorescent signal acquisition method is characterized by comprising the following steps:
adjusting the imaging surface of the linear array camera to be parallel to the area of the line to be acquired on the chip;
the linear array camera acquires an image of the line area to be acquired and controls the relative motion of the chip and the linear array camera;
and performing data processing on the image to acquire a digital PCR fluorescent signal.
2. The method for acquiring the digital PCR fluorescent signal according to claim 1, wherein the adjusting that the imaging surface of the line camera is parallel to the region of the line to be acquired on the chip comprises:
placing the chip on a bottom plate;
adjusting the linear array camera to rotate along a first direction so that an imaging surface of the linear array camera is parallel to a line region to be acquired on a chip;
the first direction is parallel to the linear array arrangement direction of the linear array camera and is parallel to the plane of the bottom plate.
3. The method for acquiring digital PCR fluorescent signals according to claim 1, wherein the controlling the relative movement of the chip and the line camera comprises:
controlling the chip and/or the linear array camera to translate relatively;
the linear array camera synchronously acquires an image of the chip once every time the linear array camera translates relatively;
and repeating the process until all images of the chip are acquired.
4. The method for acquiring digital PCR fluorescent signals according to claim 1, wherein the data processing the image to acquire digital PCR fluorescent signals comprises:
and after at least two images of the chip are collected, the computer reads the images once, and data processing is carried out on the obtained images to obtain digital PCR fluorescent signals.
5. A digital PCR fluorescence signal acquisition apparatus, comprising:
the adjusting module is used for adjusting the imaging surface of the linear array camera to be parallel to the area of the line to be acquired on the chip;
the acquisition module is used for controlling the linear array camera to acquire the image of the line area to be acquired;
the control module is used for controlling the relative motion of the chip and the linear array camera;
and the processing module is used for carrying out data processing on the image so as to obtain a digital PCR fluorescent signal.
6. The digital PCR fluorescent signal acquisition device according to claim 5, wherein the adjusting module is specifically configured to:
placing the chip on a bottom plate;
adjusting the linear array camera to rotate along a first direction so that an imaging surface of the linear array camera is parallel to a line region to be acquired on a chip;
the first direction is parallel to the linear array arrangement direction of the linear array camera and is parallel to the plane of the bottom plate.
7. The digital PCR fluorescent signal acquisition device according to claim 5, wherein the control module is specifically configured to:
and controlling the chip and/or the linear array camera to translate relatively.
8. The digital PCR fluorescent signal acquisition device according to claim 5, wherein the processing module is specifically configured to:
and after at least two images of the chip are collected, the computer reads the images once, and data processing is carried out on the obtained images to obtain digital PCR fluorescent signals.
9. A digital PCR fluorescence signal acquisition apparatus, comprising:
one or more processors;
storage means for storing one or more programs;
when executed by the one or more processors, cause the one or more processors to implement the digital PCR fluorescence signal acquisition method of any one of claims 1-4.
10. A computer-readable storage medium, on which a computer program is stored, which, when being executed by a processor, implements the digital PCR fluorescence signal acquisition method according to any one of claims 1 to 4.
CN202011482833.8A 2020-12-15 2020-12-15 Digital PCR fluorescent signal acquisition method, device, equipment and storage medium Pending CN112652360A (en)

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Citations (5)

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CN101751659A (en) * 2009-12-24 2010-06-23 北京优纳科技有限公司 Large-volume rapid image splicing method
US20110115897A1 (en) * 2008-10-24 2011-05-19 Aperio Technologies, Inc. Whole Slide Fluorescence Scanner
CN102274045A (en) * 2011-05-27 2011-12-14 华南理工大学 Parallel real-time medical ultrasonic wide-scene imaging method
CN107254511A (en) * 2017-04-27 2017-10-17 臻准生物科技（上海）有限公司 A kind of digital pcr chip signal read method
CN111598771A (en) * 2020-01-15 2020-08-28 电子科技大学 PCB (printed Circuit Board) defect detection system and method based on CCD (Charge coupled device) camera

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110115897A1 (en) * 2008-10-24 2011-05-19 Aperio Technologies, Inc. Whole Slide Fluorescence Scanner
CN101751659A (en) * 2009-12-24 2010-06-23 北京优纳科技有限公司 Large-volume rapid image splicing method
CN102274045A (en) * 2011-05-27 2011-12-14 华南理工大学 Parallel real-time medical ultrasonic wide-scene imaging method
CN107254511A (en) * 2017-04-27 2017-10-17 臻准生物科技（上海）有限公司 A kind of digital pcr chip signal read method
CN111598771A (en) * 2020-01-15 2020-08-28 电子科技大学 PCB (printed Circuit Board) defect detection system and method based on CCD (Charge coupled device) camera

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